Kazys Sadauskas | Lithuanian University of Educational Science (original) (raw)
Papers by Kazys Sadauskas
Sustainability, 2020
Due to unique chemical properties, rare earth elements (REEs) are increasingly used in versatile ... more Due to unique chemical properties, rare earth elements (REEs) are increasingly used in versatile technological applications. They are considered emerging environmental contaminants, since they become mobile instead of being bound in rocks. At present, the information on REE effects to aquatic biota is scarce and contradictory. This study aims to explore the ecotoxicity of 11 lanthanides (La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Er, and Lu) and yttrium (Y) to charophyte algae Nitellopsis obtusa and microcrustaceans Thamnocephalus platyurus. Median lethal concentrations (LC50) were assessed in characean cells at 8, 12, 16, 20, and 24 days of exposure, and 24-h LC50s were determined in shrimps. According to the EU−Directive 93/67/EEC hazard classification scheme and 24-day LC50 values generated for N. obtusa, REE effects were assigned from “harmful” to “very toxic” (Gd), while 24-h LC50s for T. platyurus were classified as “harmful” or “toxic” (based on nominal concentrations) and as “toxic...
Botanica Lithuanica, 2015
The effects of 5 s–24 h exposure to CuO nanosuspension (nCuO) and CuSO4 on mortality of Nitellops... more The effects of 5 s–24 h exposure to CuO nanosuspension (nCuO) and CuSO4 on mortality of Nitellopsis obtusa cells within 48 days were investigated. Based on LED50 kinetics, a toxicological endpoint of lethal exposure duration that induces 50% cell mortality, it was shown that mortality response of 10-min treated cells (by 3.2 and 24–79.9 mg Cu/l, respectively CuSO4 and nCuO) did not differ after 12th day rewash in control medium. Larger amount of total Cu (in mg/g cell DM) was measured in charophyte cells exposed to nCuO (79.9 mg Cu/l) than to ionic Cu (3.2 mg Cu/l). The lower nCuO-induced mortality than that of Cu2+ during earlier rewash period could be related to the sorption of nanoparticles on the cell wall, which was confirmed by scanning electron microscope images and energy dispersive X-ray spectrum data. Effect threshold, the shortest exposure duration that induces cell mortality significantly different from the control during a post-exposure period, comprised 30 s in 24 mg C...
Environmental Science and Pollution Research, 2016
Cu accumulation in the internodal cell of charophyte Nitellopsis obtusa or its compartments was i... more Cu accumulation in the internodal cell of charophyte Nitellopsis obtusa or its compartments was investigated after 3-h-exposure to lethal effective concentrations (8-day LC50) of CuO nanoparticle (nCuO) suspension or CuSO4 solution, i.e. 100 mg/L nCuO or 3.18 mg Cu/L as CuSO4. In both cases, the major part of Cu accumulated in the cell walls. The presence of CuO NPs in the cell wall and within the cell was visualized by scanning electron microscope images as well as confirmed by energy dispersive X-ray spectrum data. Although a threefold higher intracellular concentration of Cu was found after treatment with nCuO suspension, 3.18 mg Cu/L as CuSO4 induced fast and substantial depolarization of cell membrane potential contrary to that of 100 mg/L nCuO. A delayed effect of nCuO on the survival of the cells was also observed. This suggests that internally accumulated Cu was far less active and further supports the hypothesis of delayed toxicity of internalized nCuO NPs to charophyte cells.
Science of The Total Environment, 2014
The replacement of autochthonous tree species by invasive ones in coastal zones of freshwater bod... more The replacement of autochthonous tree species by invasive ones in coastal zones of freshwater bodies induces additional alteration of hydrochemical and microbiological characteristics due to decomposition of fallen leaves of non-indigenous species, which can lead to ecotoxic response of the littoral biota. Leaves of invasive to Lithuania boxelder maple (Acer negundo) and autochthonous black alder (Alnus glutinosa) lost more than half of biomass and released stable amount of DOC (60-70 mg/L) throughout 90-day mesocosm experiment under aerobic conditions. This, along with the relatively small BOD7 values detected after some variation within the first month confirms effective biodegradation by fungi and bacteria. The ambient water was more enriched with different forms of N and P by decomposing boxelder maple than by alder leaves. During the first month, both leachates were more toxic to charophyte (Nitellopsis obtusa) at mortality and membrane depolarization levels, while later to two crustacean species. Biomarker response, H(+)-ATPase activity in membrane preparations from N. obtusa, was stronger for A. negundo. Generally, boxelder maple leaf leachates were more toxic to tested hydrobionts and this coincides with previous study on leaves of the same pair of tree species conducted under microaerobic conditions (Krevš et al., 2013).
Environmental Toxicology, 2004
A microscale bioassay based on 50% inhibition of K ϩ , Mg 2ϩ-ATPase activity in a microsomal frac... more A microscale bioassay based on 50% inhibition of K ϩ , Mg 2ϩ-ATPase activity in a microsomal fraction isolated from Nitellopsis obtusa cells was developed. Compared to that for a plasma membrane fraction purified in a sucrose gradient, the preparation procedure for a microsomal fraction is less time consuming and the yield is substantially higher. Characteristics of the microsomal preparation proved to be similar to those of the highly purified plasma membrane preparation (Manusadž ianas et al., 2002), at least for heavy metals. Sensitivity to CuSO 4 of the frozen (Ϫ8°C) microsomal fraction [49 Ϯ 17 (SD) M; n ϭ 8] did not significantly differ from that of the freshly isolated one (52 Ϯ 30, n ϭ 8), at least for 40 days. Toxicity of leachate water from Kairiai (northern Lithuania) solid waste landfill was assessed by taking samples from various points including temporary reservoirs and analyzing them immediately after spillage (summer 2002) and after storage for almost 2 years at 4°C-6°C. Two tests with the macrophytic alga Nitellopsis obtusa (Charatox, 45-min EC 50 of resting potential depolarization, and ATPase assay, IC 50 of membrane ATPase activity) and one test with the crustacean Daphnia magna (Daphtoxkit F™, 48-h 50% immobilization) tests were used. In general, all three tests showed successively decreasing values of landfill leachate toxicity with an increasing degree of dilution with surface waters. The possibility of employing preserved algal preparations on demand in test batteries seems to be promising, especially in emergencies.
Environmental Pollution, 2013
Throughout 90-day biodegradation under microaerobic conditions, invasive to Lithuania species box... more Throughout 90-day biodegradation under microaerobic conditions, invasive to Lithuania species boxelder maple (Acer negundo) leaves lost 1.5-fold more biomass than that of autochthonous black alder (Alnus glutinosa), releasing higher contents of N(tot), ammonium and generating higher BOD(7). Boxelder maple leaf leachates were characterized by higher total bacterial numbers and colony numbers of heterotrophic and cellulose-decomposing bacteria than those of black alder. The higher toxicity of A. negundo aqueous extracts and leachates to charophyte cell (Nitellopsis obtusa), the inhabitant of clean lakes, were manifested at mortality and membrane depolarization levels, while the effect on H(+)-ATPase activity in membrane preparations from the same algae was stronger in case of A. glutinosa. Duckweed (Lemna minor), a bioindicator of eutrophic waters, was more sensitive to leaf leachates of A. glutinosa. Fallen leaves and leaf litter leachates from invasive and native species of trees, which enter water body, affect differently microbial biodestruction and aquatic vegetation in freshwater systems.
Desalination, 2010
ABSTRACT Several toxicological indices currently proposed (inter)nationally to aid decision-makin... more ABSTRACT Several toxicological indices currently proposed (inter)nationally to aid decision-making tasks in effluent control were compared. To evaluate effluent ranking differences between the indices an inversion distance as a similarity measure was proposed. The major factor influencing effluent ranking were found to be a composition of test-battery. Differences in mathematical operator of the indices such as, e.g. arithmetic mean (used in averaging indices) or maximum one (used in an approach of the most sensitive test in a battery), affect the ranking similarity noticeably lesser than the modification of the percentage effect level of the measurement endpoints. Effluent and/or river flow parameters incorporated into the indices substantially modify ranking results of the effluent set. For the analysis, the toxicological information on 23 effluents from municipal wastewater treatment plants operating in cities and small towns of Lithuania as well as test-battery data on industrial effluents published elsewhere were used. It seems that the general statements achieved in the current study can be applied to any set of effluents.
Aquatic Toxicology, 2003
The toxicity of industrial and urban wastewater (WW) samples collected in Lithuania and Estonia w... more The toxicity of industrial and urban wastewater (WW) samples collected in Lithuania and Estonia was evaluated by using a suite of biological tests comprising the Algaltoxkit F with Selenastrum capricornutum, the Charatox with Nitellopsis obtusa, Daphtoxkit F with Daphnia magna, Thamnotoxkit F with Thamnocephalus platyurus, Protoxkit F with Tetrahymena thermophila and the Microtox with Vibrio fischeri. The Charatox and Thamnotoxkit F tests showed highest relative sensitivity, responding to 80-90% of samples, respectively, and both expressed good discrimination capacity between samples. Principal Component and pairwise correlation analysis allowed to select test-battery consisting of Charatox, Thamnotoxkit and Microtox. The WW toxicity was evaluated by means of cumulative indices such as average toxicity (AvTx) and two indices derived from the PEEP-index (Environ. Toxicol. Water Qual. 8 (1993) 115). In addition to these integrated evaluations of test-battery response, WW toxicity was evaluated according to the most sensitive test (MST) in the battery. The linear regression analysis between cumulative toxicity indices and chemical-based indices (derived from comparison of WW chemical concentrations and their respective maximum allowable concentration) revealed positive linear relationships (r(2)=0.7-0.8), while toxicity evaluation based on the MST was less positively related with chemical analysis data (r(2)=0.5-0.6). Although better coincidence between the toxicity and chemical-based assessments was achieved when information from all tests in the battery was assembled, the prediction of toxicity from chemical data was still limited. In search of suitable test-battery for the screening of certain type of WWs, a preliminary study comprising excessive suite of tests might be useful.
Environmental Toxicology and Chemistry, 2011
Toxicity effects induced by nanosuspensions of CuO (<50 nm; Sigma-Aldrich) on macrophytic algae c... more Toxicity effects induced by nanosuspensions of CuO (<50 nm; Sigma-Aldrich) on macrophytic algae cells of Nitellopsis obtusa (96-h median lethal concentration [LC50]), microphytic algae Chlorella (30-min median inhibitory concentration [IC50]), shrimp Thamnocephalus platyurus (24-h LC50), and rotifer Brachionus calyciflorus (24-h LC50) were investigated. No substantial differences between the effects of nonsonicated and sonicated nCuO suspensions were observed. The particle size distribution analysis accomplished by the laser diffraction technique at suspension concentration from 3 to 100 mg/L revealed rapid (within 5 min) reagglomeration of the particles after the sonication. The observed adverse effects on N. obtusa cells may be attributed to nanoparticles per se, but not to ionic Cu, because neither chemical analysis nor biological testing (algae survival in the supernatants of suspensions) confirmed the presence of cupric ions in toxic amounts. Contrary to ionic Cu form, nCuO delayed the initial phase of N. obtusa cell membrane depolarization. Lethality tests with rewash demonstrated that the least used 5-min exposure in 100 mg/L nCuO sonicated suspension induced 70% mortality in charophyte cells after 8 d, whereas the rewash after a short exposure to a noticeably toxic concentration of Cu 2þ prevented cell mortality. The obtained data suggested the possible influence of a thick charophyte cell wall on the dynamics of nanotoxicity effects.
Environmental Toxicology, 2002
The responses of the freshwater macroalga Nitellopsis obtusa to heavy metal (HM) salts of Hg, Cd,... more The responses of the freshwater macroalga Nitellopsis obtusa to heavy metal (HM) salts of Hg, Cd, Co, Cu, Cr, and Ni were assessed at different levels: whole-cell mortality (96-h LC(50)), in vivo cell membrane (45-min depolarization of resting potential, EC(50)), and enzyme in plasma membrane preparations (K+, Mg2+-specific H+-ATPase inhibition, IC(50)). To measure ATPase activity, a novel procedure for isolation of plasma membrane-enriched vesicles from charophyte cells was developed. The short-term ATPase inhibition assay (IC(50) from 6.0 x 10(-7) to 4.6 x 10(-4) M) was slightly more sensitive than the cell mortality test (LC(50) from 1.1 x 10(-6) to 2.6 x 10(-3) M), and the electrophysiological test with the end point of 45-min depolarization of resting potential was characterized by less sensitivity for HMs (EC(50) from 1.1 x 10(-4) to 2.2 x 10(-2) M). The variability of IC(50) values assessed for HMs in the ATPase assays was close to that of LC(50) values in the mortality tests (CVs from 33.5 to 83.5 and from 12.4% to 57.7%, respectively), whereas the EC(50) values in the electrophysiological tests were characterized by CVs generally below 30%. All three end points identified two separate HM groups according to their toxicity to N. obtusa: Co, Ni, and Cr comprised a group of less toxic metals, whereas Hg, Cu, and Cd comprised a group of more toxic metals. However, the adverse effects within each group were discriminated differently. For example, the maximum difference between the highest and lowest LC(50) for the group of less toxic metals in the long-term mortality test was approximately 60% of the response range, whereas the corresponding difference in IC(50) values in the ATPase assay was 30%. In contrast, the LC(50) values of the more toxic metals occupied only 10% of the response range, whereas the IC(50) values were spread over 70%. Further investigation should be done of the underlying mechanism or mechanisms responsible for the observed differences in the dynamic range of a particular end point of the groups of toxicants of varying strength.
PeerJ
Invasive plant species tend to migrate from their native habitats under favourable climatic condi... more Invasive plant species tend to migrate from their native habitats under favourable climatic conditions; therefore, trophic and other relationships in ecosystems are changing. To investigate the effect of natural organic matter derived from native Alnus glutinosa tree species and from invasive in Lithuania Acer negundo tree species on copper toxicity in Lemna minor, we analysed the dynamics of Cu binding in aqueous leaf litter extracts (LLE) and plant accumulation, morphophysiological parameters, and antioxidative response. The results revealed that A. glutinosa LLE contained polyphenols (49 mg pyrogallol acid equivalent (PAE)/g DM) and tannins (7.5 mg PAE/g DM), while A. negundo LLE contained only polyphenols (23 mg PAE/g DM). The ability of LLE to bind Cu increased rapidly over 1.5–3 h to 61% and 49% of the total Cu concentration (6.0 ± 0.9 mg/L), respectively for A. glutinosa (AG) and A. negundo (AN), then remained relatively stable until 48 h. At the same time, L. minor accumulat...
Aquatic Toxicology, 2003
The toxicity of industrial and urban wastewater (WW) samples collected in Lithuania and Estonia w... more The toxicity of industrial and urban wastewater (WW) samples collected in Lithuania and Estonia was evaluated by using a suite of biological tests comprising the Algaltoxkit F with Selenastrum capricornutum, the Charatox with Nitellopsis obtusa, Daphtoxkit F with Daphnia magna, Thamnotoxkit F with Thamnocephalus platyurus, Protoxkit F with Tetrahymena thermophila and the Microtox with Vibrio fischeri. The Charatox and Thamnotoxkit F tests showed highest relative sensitivity, responding to 80-90% of samples, respectively, and both expressed good discrimination capacity between samples. Principal Component and pairwise correlation analysis allowed to select test-battery consisting of Charatox, Thamnotoxkit and Microtox. The WW toxicity was evaluated by means of cumulative indices such as average toxicity (AvTx) and two indices derived from the PEEP-index (Environ. Toxicol. Water Qual. 8 (1993) 115). In addition to these integrated evaluations of test-battery response, WW toxicity was evaluated according to the most sensitive test (MST) in the battery. The linear regression analysis between cumulative toxicity indices and chemical-based indices (derived from comparison of WW chemical concentrations and their respective maximum allowable concentration) revealed positive linear relationships (r(2)=0.7-0.8), while toxicity evaluation based on the MST was less positively related with chemical analysis data (r(2)=0.5-0.6). Although better coincidence between the toxicity and chemical-based assessments was achieved when information from all tests in the battery was assembled, the prediction of toxicity from chemical data was still limited. In search of suitable test-battery for the screening of certain type of WWs, a preliminary study comprising excessive suite of tests might be useful.
Zhurnal vyssheĭ nervnoĭ deiatelnosti imeni I P Pavlova
New Microbiotests for Routine Toxicity Screening and Biomonitoring, 2000
Science of The Total Environment, 2014
The replacement of autochthonous tree species by invasive ones in coastal zones of freshwater bod... more The replacement of autochthonous tree species by invasive ones in coastal zones of freshwater bodies induces additional alteration of hydrochemical and microbiological characteristics due to decomposition of fallen leaves of non-indigenous species, which can lead to ecotoxic response of the littoral biota. Leaves of invasive to Lithuania boxelder maple (Acer negundo) and autochthonous black alder (Alnus glutinosa) lost more than half of biomass and released stable amount of DOC (60-70 mg/L) throughout 90-day mesocosm experiment under aerobic conditions. This, along with the relatively small BOD 7 values detected after some variation within the first month confirms effective biodegradation by fungi and bacteria. The ambient water was more enriched with different forms of N and P by decomposing boxelder maple than by alder leaves. During the first month, both leachates were more toxic to charophyte (Nitellopsis obtusa) at mortality and membrane depolarization levels, while later to two crustacean species. Biomarker response, H + -ATPase activity in membrane preparations from N. obtusa, was stronger for A. negundo. Generally, boxelder maple leaf leachates were more toxic to tested hydrobionts and this coincides with previous study on leaves of the same pair of tree species conducted under microaerobic conditions .
Environmental Toxicology and Chemistry, 2012
Toxicity effects induced by nanosuspensions of CuO (<50 nm; Sigma-Aldrich) on macrophytic algae c... more Toxicity effects induced by nanosuspensions of CuO (<50 nm; Sigma-Aldrich) on macrophytic algae cells of Nitellopsis obtusa (96-h median lethal concentration [LC50]), microphytic algae Chlorella (30-min median inhibitory concentration [IC50]),
Environmental Toxicology, 2002
Environmental Pollution, 2013
Throughout 90-day biodegradation under microaerobic conditions, invasive to Lithuania species box... more Throughout 90-day biodegradation under microaerobic conditions, invasive to Lithuania species boxelder maple (Acer negundo) leaves lost 1.5-fold more biomass than that of autochthonous black alder (Alnus glutinosa), releasing higher contents of N tot , ammonium and generating higher BOD 7 . Boxelder maple leaf leachates were characterized by higher total bacterial numbers and colony numbers of heterotrophic and cellulose-decomposing bacteria than those of black alder. The higher toxicity of A. negundo aqueous extracts and leachates to charophyte cell (Nitellopsis obtusa), the inhabitant of clean lakes, were manifested at mortality and membrane depolarization levels, while the effect on H þ -ATPase activity in membrane preparations from the same algae was stronger in case of A. glutinosa. Duckweed (Lemna minor), a bioindicator of eutrophic waters, was more sensitive to leaf leachates of A. glutinosa. Fallen leaves and leaf litter leachates from invasive and native species of trees, which enter water body, affect differently microbial biodestruction and aquatic vegetation in freshwater systems.
Desalination, 2010
ABSTRACT Several toxicological indices currently proposed (inter)nationally to aid decision-makin... more ABSTRACT Several toxicological indices currently proposed (inter)nationally to aid decision-making tasks in effluent control were compared. To evaluate effluent ranking differences between the indices an inversion distance as a similarity measure was proposed. The major factor influencing effluent ranking were found to be a composition of test-battery. Differences in mathematical operator of the indices such as, e.g. arithmetic mean (used in averaging indices) or maximum one (used in an approach of the most sensitive test in a battery), affect the ranking similarity noticeably lesser than the modification of the percentage effect level of the measurement endpoints. Effluent and/or river flow parameters incorporated into the indices substantially modify ranking results of the effluent set. For the analysis, the toxicological information on 23 effluents from municipal wastewater treatment plants operating in cities and small towns of Lithuania as well as test-battery data on industrial effluents published elsewhere were used. It seems that the general statements achieved in the current study can be applied to any set of effluents.
Aquatic Toxicology, 2003
The toxicity of industrial and urban wastewater (WW) samples collected in Lithuania and Estonia w... more The toxicity of industrial and urban wastewater (WW) samples collected in Lithuania and Estonia was evaluated by using a suite of biological tests comprising the Algaltoxkit F with Selenastrum capricornutum, the Charatox with Nitellopsis obtusa, Daphtoxkit F with Daphnia magna, Thamnotoxkit F with Thamnocephalus platyurus, Protoxkit F with Tetrahymena thermophila and the Microtox with Vibrio fischeri. The Charatox and Thamnotoxkit F tests showed highest relative sensitivity, responding to 80-90% of samples, respectively, and both expressed good discrimination capacity between samples. Principal Component and pairwise correlation analysis allowed to select test-battery consisting of Charatox, Thamnotoxkit and Microtox. The WW toxicity was evaluated by means of cumulative indices such as average toxicity (AvTx) and two indices derived from the PEEP-index (Environ. Toxicol. Water Qual. 8 (1993) 115). In addition to these integrated evaluations of test-battery response, WW toxicity was evaluated according to the most sensitive test (MST) in the battery. The linear regression analysis between cumulative toxicity indices and chemical-based indices (derived from comparison of WW chemical concentrations and their respective maximum allowable concentration) revealed positive linear relationships (r(2)=0.7-0.8), while toxicity evaluation based on the MST was less positively related with chemical analysis data (r(2)=0.5-0.6). Although better coincidence between the toxicity and chemical-based assessments was achieved when information from all tests in the battery was assembled, the prediction of toxicity from chemical data was still limited. In search of suitable test-battery for the screening of certain type of WWs, a preliminary study comprising excessive suite of tests might be useful.
Sustainability, 2020
Due to unique chemical properties, rare earth elements (REEs) are increasingly used in versatile ... more Due to unique chemical properties, rare earth elements (REEs) are increasingly used in versatile technological applications. They are considered emerging environmental contaminants, since they become mobile instead of being bound in rocks. At present, the information on REE effects to aquatic biota is scarce and contradictory. This study aims to explore the ecotoxicity of 11 lanthanides (La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Er, and Lu) and yttrium (Y) to charophyte algae Nitellopsis obtusa and microcrustaceans Thamnocephalus platyurus. Median lethal concentrations (LC50) were assessed in characean cells at 8, 12, 16, 20, and 24 days of exposure, and 24-h LC50s were determined in shrimps. According to the EU−Directive 93/67/EEC hazard classification scheme and 24-day LC50 values generated for N. obtusa, REE effects were assigned from “harmful” to “very toxic” (Gd), while 24-h LC50s for T. platyurus were classified as “harmful” or “toxic” (based on nominal concentrations) and as “toxic...
Botanica Lithuanica, 2015
The effects of 5 s–24 h exposure to CuO nanosuspension (nCuO) and CuSO4 on mortality of Nitellops... more The effects of 5 s–24 h exposure to CuO nanosuspension (nCuO) and CuSO4 on mortality of Nitellopsis obtusa cells within 48 days were investigated. Based on LED50 kinetics, a toxicological endpoint of lethal exposure duration that induces 50% cell mortality, it was shown that mortality response of 10-min treated cells (by 3.2 and 24–79.9 mg Cu/l, respectively CuSO4 and nCuO) did not differ after 12th day rewash in control medium. Larger amount of total Cu (in mg/g cell DM) was measured in charophyte cells exposed to nCuO (79.9 mg Cu/l) than to ionic Cu (3.2 mg Cu/l). The lower nCuO-induced mortality than that of Cu2+ during earlier rewash period could be related to the sorption of nanoparticles on the cell wall, which was confirmed by scanning electron microscope images and energy dispersive X-ray spectrum data. Effect threshold, the shortest exposure duration that induces cell mortality significantly different from the control during a post-exposure period, comprised 30 s in 24 mg C...
Environmental Science and Pollution Research, 2016
Cu accumulation in the internodal cell of charophyte Nitellopsis obtusa or its compartments was i... more Cu accumulation in the internodal cell of charophyte Nitellopsis obtusa or its compartments was investigated after 3-h-exposure to lethal effective concentrations (8-day LC50) of CuO nanoparticle (nCuO) suspension or CuSO4 solution, i.e. 100 mg/L nCuO or 3.18 mg Cu/L as CuSO4. In both cases, the major part of Cu accumulated in the cell walls. The presence of CuO NPs in the cell wall and within the cell was visualized by scanning electron microscope images as well as confirmed by energy dispersive X-ray spectrum data. Although a threefold higher intracellular concentration of Cu was found after treatment with nCuO suspension, 3.18 mg Cu/L as CuSO4 induced fast and substantial depolarization of cell membrane potential contrary to that of 100 mg/L nCuO. A delayed effect of nCuO on the survival of the cells was also observed. This suggests that internally accumulated Cu was far less active and further supports the hypothesis of delayed toxicity of internalized nCuO NPs to charophyte cells.
Science of The Total Environment, 2014
The replacement of autochthonous tree species by invasive ones in coastal zones of freshwater bod... more The replacement of autochthonous tree species by invasive ones in coastal zones of freshwater bodies induces additional alteration of hydrochemical and microbiological characteristics due to decomposition of fallen leaves of non-indigenous species, which can lead to ecotoxic response of the littoral biota. Leaves of invasive to Lithuania boxelder maple (Acer negundo) and autochthonous black alder (Alnus glutinosa) lost more than half of biomass and released stable amount of DOC (60-70 mg/L) throughout 90-day mesocosm experiment under aerobic conditions. This, along with the relatively small BOD7 values detected after some variation within the first month confirms effective biodegradation by fungi and bacteria. The ambient water was more enriched with different forms of N and P by decomposing boxelder maple than by alder leaves. During the first month, both leachates were more toxic to charophyte (Nitellopsis obtusa) at mortality and membrane depolarization levels, while later to two crustacean species. Biomarker response, H(+)-ATPase activity in membrane preparations from N. obtusa, was stronger for A. negundo. Generally, boxelder maple leaf leachates were more toxic to tested hydrobionts and this coincides with previous study on leaves of the same pair of tree species conducted under microaerobic conditions (Krevš et al., 2013).
Environmental Toxicology, 2004
A microscale bioassay based on 50% inhibition of K ϩ , Mg 2ϩ-ATPase activity in a microsomal frac... more A microscale bioassay based on 50% inhibition of K ϩ , Mg 2ϩ-ATPase activity in a microsomal fraction isolated from Nitellopsis obtusa cells was developed. Compared to that for a plasma membrane fraction purified in a sucrose gradient, the preparation procedure for a microsomal fraction is less time consuming and the yield is substantially higher. Characteristics of the microsomal preparation proved to be similar to those of the highly purified plasma membrane preparation (Manusadž ianas et al., 2002), at least for heavy metals. Sensitivity to CuSO 4 of the frozen (Ϫ8°C) microsomal fraction [49 Ϯ 17 (SD) M; n ϭ 8] did not significantly differ from that of the freshly isolated one (52 Ϯ 30, n ϭ 8), at least for 40 days. Toxicity of leachate water from Kairiai (northern Lithuania) solid waste landfill was assessed by taking samples from various points including temporary reservoirs and analyzing them immediately after spillage (summer 2002) and after storage for almost 2 years at 4°C-6°C. Two tests with the macrophytic alga Nitellopsis obtusa (Charatox, 45-min EC 50 of resting potential depolarization, and ATPase assay, IC 50 of membrane ATPase activity) and one test with the crustacean Daphnia magna (Daphtoxkit F™, 48-h 50% immobilization) tests were used. In general, all three tests showed successively decreasing values of landfill leachate toxicity with an increasing degree of dilution with surface waters. The possibility of employing preserved algal preparations on demand in test batteries seems to be promising, especially in emergencies.
Environmental Pollution, 2013
Throughout 90-day biodegradation under microaerobic conditions, invasive to Lithuania species box... more Throughout 90-day biodegradation under microaerobic conditions, invasive to Lithuania species boxelder maple (Acer negundo) leaves lost 1.5-fold more biomass than that of autochthonous black alder (Alnus glutinosa), releasing higher contents of N(tot), ammonium and generating higher BOD(7). Boxelder maple leaf leachates were characterized by higher total bacterial numbers and colony numbers of heterotrophic and cellulose-decomposing bacteria than those of black alder. The higher toxicity of A. negundo aqueous extracts and leachates to charophyte cell (Nitellopsis obtusa), the inhabitant of clean lakes, were manifested at mortality and membrane depolarization levels, while the effect on H(+)-ATPase activity in membrane preparations from the same algae was stronger in case of A. glutinosa. Duckweed (Lemna minor), a bioindicator of eutrophic waters, was more sensitive to leaf leachates of A. glutinosa. Fallen leaves and leaf litter leachates from invasive and native species of trees, which enter water body, affect differently microbial biodestruction and aquatic vegetation in freshwater systems.
Desalination, 2010
ABSTRACT Several toxicological indices currently proposed (inter)nationally to aid decision-makin... more ABSTRACT Several toxicological indices currently proposed (inter)nationally to aid decision-making tasks in effluent control were compared. To evaluate effluent ranking differences between the indices an inversion distance as a similarity measure was proposed. The major factor influencing effluent ranking were found to be a composition of test-battery. Differences in mathematical operator of the indices such as, e.g. arithmetic mean (used in averaging indices) or maximum one (used in an approach of the most sensitive test in a battery), affect the ranking similarity noticeably lesser than the modification of the percentage effect level of the measurement endpoints. Effluent and/or river flow parameters incorporated into the indices substantially modify ranking results of the effluent set. For the analysis, the toxicological information on 23 effluents from municipal wastewater treatment plants operating in cities and small towns of Lithuania as well as test-battery data on industrial effluents published elsewhere were used. It seems that the general statements achieved in the current study can be applied to any set of effluents.
Aquatic Toxicology, 2003
The toxicity of industrial and urban wastewater (WW) samples collected in Lithuania and Estonia w... more The toxicity of industrial and urban wastewater (WW) samples collected in Lithuania and Estonia was evaluated by using a suite of biological tests comprising the Algaltoxkit F with Selenastrum capricornutum, the Charatox with Nitellopsis obtusa, Daphtoxkit F with Daphnia magna, Thamnotoxkit F with Thamnocephalus platyurus, Protoxkit F with Tetrahymena thermophila and the Microtox with Vibrio fischeri. The Charatox and Thamnotoxkit F tests showed highest relative sensitivity, responding to 80-90% of samples, respectively, and both expressed good discrimination capacity between samples. Principal Component and pairwise correlation analysis allowed to select test-battery consisting of Charatox, Thamnotoxkit and Microtox. The WW toxicity was evaluated by means of cumulative indices such as average toxicity (AvTx) and two indices derived from the PEEP-index (Environ. Toxicol. Water Qual. 8 (1993) 115). In addition to these integrated evaluations of test-battery response, WW toxicity was evaluated according to the most sensitive test (MST) in the battery. The linear regression analysis between cumulative toxicity indices and chemical-based indices (derived from comparison of WW chemical concentrations and their respective maximum allowable concentration) revealed positive linear relationships (r(2)=0.7-0.8), while toxicity evaluation based on the MST was less positively related with chemical analysis data (r(2)=0.5-0.6). Although better coincidence between the toxicity and chemical-based assessments was achieved when information from all tests in the battery was assembled, the prediction of toxicity from chemical data was still limited. In search of suitable test-battery for the screening of certain type of WWs, a preliminary study comprising excessive suite of tests might be useful.
Environmental Toxicology and Chemistry, 2011
Toxicity effects induced by nanosuspensions of CuO (<50 nm; Sigma-Aldrich) on macrophytic algae c... more Toxicity effects induced by nanosuspensions of CuO (<50 nm; Sigma-Aldrich) on macrophytic algae cells of Nitellopsis obtusa (96-h median lethal concentration [LC50]), microphytic algae Chlorella (30-min median inhibitory concentration [IC50]), shrimp Thamnocephalus platyurus (24-h LC50), and rotifer Brachionus calyciflorus (24-h LC50) were investigated. No substantial differences between the effects of nonsonicated and sonicated nCuO suspensions were observed. The particle size distribution analysis accomplished by the laser diffraction technique at suspension concentration from 3 to 100 mg/L revealed rapid (within 5 min) reagglomeration of the particles after the sonication. The observed adverse effects on N. obtusa cells may be attributed to nanoparticles per se, but not to ionic Cu, because neither chemical analysis nor biological testing (algae survival in the supernatants of suspensions) confirmed the presence of cupric ions in toxic amounts. Contrary to ionic Cu form, nCuO delayed the initial phase of N. obtusa cell membrane depolarization. Lethality tests with rewash demonstrated that the least used 5-min exposure in 100 mg/L nCuO sonicated suspension induced 70% mortality in charophyte cells after 8 d, whereas the rewash after a short exposure to a noticeably toxic concentration of Cu 2þ prevented cell mortality. The obtained data suggested the possible influence of a thick charophyte cell wall on the dynamics of nanotoxicity effects.
Environmental Toxicology, 2002
The responses of the freshwater macroalga Nitellopsis obtusa to heavy metal (HM) salts of Hg, Cd,... more The responses of the freshwater macroalga Nitellopsis obtusa to heavy metal (HM) salts of Hg, Cd, Co, Cu, Cr, and Ni were assessed at different levels: whole-cell mortality (96-h LC(50)), in vivo cell membrane (45-min depolarization of resting potential, EC(50)), and enzyme in plasma membrane preparations (K+, Mg2+-specific H+-ATPase inhibition, IC(50)). To measure ATPase activity, a novel procedure for isolation of plasma membrane-enriched vesicles from charophyte cells was developed. The short-term ATPase inhibition assay (IC(50) from 6.0 x 10(-7) to 4.6 x 10(-4) M) was slightly more sensitive than the cell mortality test (LC(50) from 1.1 x 10(-6) to 2.6 x 10(-3) M), and the electrophysiological test with the end point of 45-min depolarization of resting potential was characterized by less sensitivity for HMs (EC(50) from 1.1 x 10(-4) to 2.2 x 10(-2) M). The variability of IC(50) values assessed for HMs in the ATPase assays was close to that of LC(50) values in the mortality tests (CVs from 33.5 to 83.5 and from 12.4% to 57.7%, respectively), whereas the EC(50) values in the electrophysiological tests were characterized by CVs generally below 30%. All three end points identified two separate HM groups according to their toxicity to N. obtusa: Co, Ni, and Cr comprised a group of less toxic metals, whereas Hg, Cu, and Cd comprised a group of more toxic metals. However, the adverse effects within each group were discriminated differently. For example, the maximum difference between the highest and lowest LC(50) for the group of less toxic metals in the long-term mortality test was approximately 60% of the response range, whereas the corresponding difference in IC(50) values in the ATPase assay was 30%. In contrast, the LC(50) values of the more toxic metals occupied only 10% of the response range, whereas the IC(50) values were spread over 70%. Further investigation should be done of the underlying mechanism or mechanisms responsible for the observed differences in the dynamic range of a particular end point of the groups of toxicants of varying strength.
PeerJ
Invasive plant species tend to migrate from their native habitats under favourable climatic condi... more Invasive plant species tend to migrate from their native habitats under favourable climatic conditions; therefore, trophic and other relationships in ecosystems are changing. To investigate the effect of natural organic matter derived from native Alnus glutinosa tree species and from invasive in Lithuania Acer negundo tree species on copper toxicity in Lemna minor, we analysed the dynamics of Cu binding in aqueous leaf litter extracts (LLE) and plant accumulation, morphophysiological parameters, and antioxidative response. The results revealed that A. glutinosa LLE contained polyphenols (49 mg pyrogallol acid equivalent (PAE)/g DM) and tannins (7.5 mg PAE/g DM), while A. negundo LLE contained only polyphenols (23 mg PAE/g DM). The ability of LLE to bind Cu increased rapidly over 1.5–3 h to 61% and 49% of the total Cu concentration (6.0 ± 0.9 mg/L), respectively for A. glutinosa (AG) and A. negundo (AN), then remained relatively stable until 48 h. At the same time, L. minor accumulat...
Aquatic Toxicology, 2003
The toxicity of industrial and urban wastewater (WW) samples collected in Lithuania and Estonia w... more The toxicity of industrial and urban wastewater (WW) samples collected in Lithuania and Estonia was evaluated by using a suite of biological tests comprising the Algaltoxkit F with Selenastrum capricornutum, the Charatox with Nitellopsis obtusa, Daphtoxkit F with Daphnia magna, Thamnotoxkit F with Thamnocephalus platyurus, Protoxkit F with Tetrahymena thermophila and the Microtox with Vibrio fischeri. The Charatox and Thamnotoxkit F tests showed highest relative sensitivity, responding to 80-90% of samples, respectively, and both expressed good discrimination capacity between samples. Principal Component and pairwise correlation analysis allowed to select test-battery consisting of Charatox, Thamnotoxkit and Microtox. The WW toxicity was evaluated by means of cumulative indices such as average toxicity (AvTx) and two indices derived from the PEEP-index (Environ. Toxicol. Water Qual. 8 (1993) 115). In addition to these integrated evaluations of test-battery response, WW toxicity was evaluated according to the most sensitive test (MST) in the battery. The linear regression analysis between cumulative toxicity indices and chemical-based indices (derived from comparison of WW chemical concentrations and their respective maximum allowable concentration) revealed positive linear relationships (r(2)=0.7-0.8), while toxicity evaluation based on the MST was less positively related with chemical analysis data (r(2)=0.5-0.6). Although better coincidence between the toxicity and chemical-based assessments was achieved when information from all tests in the battery was assembled, the prediction of toxicity from chemical data was still limited. In search of suitable test-battery for the screening of certain type of WWs, a preliminary study comprising excessive suite of tests might be useful.
Zhurnal vyssheĭ nervnoĭ deiatelnosti imeni I P Pavlova
New Microbiotests for Routine Toxicity Screening and Biomonitoring, 2000
Science of The Total Environment, 2014
The replacement of autochthonous tree species by invasive ones in coastal zones of freshwater bod... more The replacement of autochthonous tree species by invasive ones in coastal zones of freshwater bodies induces additional alteration of hydrochemical and microbiological characteristics due to decomposition of fallen leaves of non-indigenous species, which can lead to ecotoxic response of the littoral biota. Leaves of invasive to Lithuania boxelder maple (Acer negundo) and autochthonous black alder (Alnus glutinosa) lost more than half of biomass and released stable amount of DOC (60-70 mg/L) throughout 90-day mesocosm experiment under aerobic conditions. This, along with the relatively small BOD 7 values detected after some variation within the first month confirms effective biodegradation by fungi and bacteria. The ambient water was more enriched with different forms of N and P by decomposing boxelder maple than by alder leaves. During the first month, both leachates were more toxic to charophyte (Nitellopsis obtusa) at mortality and membrane depolarization levels, while later to two crustacean species. Biomarker response, H + -ATPase activity in membrane preparations from N. obtusa, was stronger for A. negundo. Generally, boxelder maple leaf leachates were more toxic to tested hydrobionts and this coincides with previous study on leaves of the same pair of tree species conducted under microaerobic conditions .
Environmental Toxicology and Chemistry, 2012
Toxicity effects induced by nanosuspensions of CuO (<50 nm; Sigma-Aldrich) on macrophytic algae c... more Toxicity effects induced by nanosuspensions of CuO (<50 nm; Sigma-Aldrich) on macrophytic algae cells of Nitellopsis obtusa (96-h median lethal concentration [LC50]), microphytic algae Chlorella (30-min median inhibitory concentration [IC50]),
Environmental Toxicology, 2002
Environmental Pollution, 2013
Throughout 90-day biodegradation under microaerobic conditions, invasive to Lithuania species box... more Throughout 90-day biodegradation under microaerobic conditions, invasive to Lithuania species boxelder maple (Acer negundo) leaves lost 1.5-fold more biomass than that of autochthonous black alder (Alnus glutinosa), releasing higher contents of N tot , ammonium and generating higher BOD 7 . Boxelder maple leaf leachates were characterized by higher total bacterial numbers and colony numbers of heterotrophic and cellulose-decomposing bacteria than those of black alder. The higher toxicity of A. negundo aqueous extracts and leachates to charophyte cell (Nitellopsis obtusa), the inhabitant of clean lakes, were manifested at mortality and membrane depolarization levels, while the effect on H þ -ATPase activity in membrane preparations from the same algae was stronger in case of A. glutinosa. Duckweed (Lemna minor), a bioindicator of eutrophic waters, was more sensitive to leaf leachates of A. glutinosa. Fallen leaves and leaf litter leachates from invasive and native species of trees, which enter water body, affect differently microbial biodestruction and aquatic vegetation in freshwater systems.
Desalination, 2010
ABSTRACT Several toxicological indices currently proposed (inter)nationally to aid decision-makin... more ABSTRACT Several toxicological indices currently proposed (inter)nationally to aid decision-making tasks in effluent control were compared. To evaluate effluent ranking differences between the indices an inversion distance as a similarity measure was proposed. The major factor influencing effluent ranking were found to be a composition of test-battery. Differences in mathematical operator of the indices such as, e.g. arithmetic mean (used in averaging indices) or maximum one (used in an approach of the most sensitive test in a battery), affect the ranking similarity noticeably lesser than the modification of the percentage effect level of the measurement endpoints. Effluent and/or river flow parameters incorporated into the indices substantially modify ranking results of the effluent set. For the analysis, the toxicological information on 23 effluents from municipal wastewater treatment plants operating in cities and small towns of Lithuania as well as test-battery data on industrial effluents published elsewhere were used. It seems that the general statements achieved in the current study can be applied to any set of effluents.
Aquatic Toxicology, 2003
The toxicity of industrial and urban wastewater (WW) samples collected in Lithuania and Estonia w... more The toxicity of industrial and urban wastewater (WW) samples collected in Lithuania and Estonia was evaluated by using a suite of biological tests comprising the Algaltoxkit F with Selenastrum capricornutum, the Charatox with Nitellopsis obtusa, Daphtoxkit F with Daphnia magna, Thamnotoxkit F with Thamnocephalus platyurus, Protoxkit F with Tetrahymena thermophila and the Microtox with Vibrio fischeri. The Charatox and Thamnotoxkit F tests showed highest relative sensitivity, responding to 80-90% of samples, respectively, and both expressed good discrimination capacity between samples. Principal Component and pairwise correlation analysis allowed to select test-battery consisting of Charatox, Thamnotoxkit and Microtox. The WW toxicity was evaluated by means of cumulative indices such as average toxicity (AvTx) and two indices derived from the PEEP-index (Environ. Toxicol. Water Qual. 8 (1993) 115). In addition to these integrated evaluations of test-battery response, WW toxicity was evaluated according to the most sensitive test (MST) in the battery. The linear regression analysis between cumulative toxicity indices and chemical-based indices (derived from comparison of WW chemical concentrations and their respective maximum allowable concentration) revealed positive linear relationships (r(2)=0.7-0.8), while toxicity evaluation based on the MST was less positively related with chemical analysis data (r(2)=0.5-0.6). Although better coincidence between the toxicity and chemical-based assessments was achieved when information from all tests in the battery was assembled, the prediction of toxicity from chemical data was still limited. In search of suitable test-battery for the screening of certain type of WWs, a preliminary study comprising excessive suite of tests might be useful.