GitHub - mleiwe/LinearUnmixing: MATLAB codes to linearly unmix 4d images (xyz and colour) (original) (raw)

MATLAB codes to linearly unmix 4d images (xyz and colour)

Requirements

mnl_Load4Dimage - available here, https://github.com/mleiwe/QDyeFinder/tree/main/SubcodesBioformats toolbox -

Functions

There are three versions of the same program

sf_unmixing_20xG_3HyD_220823ref.m - Unmixing the data obtained by Leica SP8 with 20X multi-immersion objectives, Glycerine immersion and 3 HyD detectors.

sf_unmixing_20xG_220821ref.m - Unmixing the data obtained by Leica SP8 with 20X multi-immersion objectives, Glycerine immersion and a single HyD detector.

sf_unmixing_63x_220823ref.m - Unmixing the data obtained by Leica SP8 with 63X oil-immersion objectives, oil immersion and a single HyD detector.

The structure of these codes is the same, but the parameters (ref405, ref488 and ref552) used as the references are different for each imaging condition.

How to run these codes

Type sf_unmixing_20xG_3HyD_220823ref("filename") in the command window