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Research paper thumbnail of Frequency of significant virulence genes in gastric biopsies of Helicobacter pylori-positive patients with gastritis

AMB Express

Helicobacter pylori is one of the most common bacteria affecting human societies worldwide, and i... more Helicobacter pylori is one of the most common bacteria affecting human societies worldwide, and is mainly associated with gastrointestinal complications due to different virulence factors. This study aimed to investigate some virulence genes of H. pylori in gastric biopsies of patients with gastritis in Sari city, North of Iran. Informed consent forms were obtained from the studied patients, and those who needed endoscopy were included in the study. To evaluate the prevalence of cagA, iceA1, iceA2, vacA, dupA, and oipA genes, gastric biopsies with positive or negative rapid urease test were collected from 50 patients (25 in each group) with gastro-duodenal diseases. The bacterial DNAs were extracted by a specific kit, and the presence of the genes was analyzed by PCR using specific primers. Eighteen (72%) biopsies from 25 H. pylori-positive samples were cagA-positive, while 17 (68%) biopsies contained the vacA gene, and 11 (44%) samples had both vacA and cagA genes. However, 16 (64%...

Research paper thumbnail of Effects of Estrogen and Progesterone on Behavioral Impairment and Neuronal Death in Ovariectomized Rats Induced by Methamphetamine

Journal of Mazandaran University of Medical Sciences, 2020

Background and purpose: Methamphetamine (METH) is one of the most powerful drugs that leads to ma... more Background and purpose: Methamphetamine (METH) is one of the most powerful drugs that leads to many cognitive and behavioral side effects such as anxiety. On the other hand, studies have shown that ovarian hormones such as estrogen and progesterone have neuroprotective effects on a wide range of cognitive and behavioral disorders. The aim of this study was to investigate the role of estrogen and progesterone on anxiety-like behaviors, body temperature, brain edema, and neuronal death induced by neurotoxic regimen of methamphetamine. Materials and methods: This study was performed in 48 ovariectomized rats divided into six groups including: control, METH (6mg / kg), vehicle (sesame oil), METH + estrogen (1mg / kg), METH + progesterone (8mg / kg), and METH + estrogen + progesterone. Body temperature and anxiety-like behaviors were investigated, then, the animals were killed and brain tissues were harvested to evaluate brain edema and neuronal death in hippocampal CA1. Results: Body te...

Research paper thumbnail of Effects of Clozapine and Risperidone Antipsychotic Drugs on the Expression of CACNA1C and Behavioral Changes in Rat ‘Ketamine Model of Schizophrenia

Research paper thumbnail of Procedure and Patient-Related Risk Factors for Post-ERCP Pancreatitis Associated with Prophylactic Pancreatic Stent and Rectal Indomethacin

Background: Pancreatitis is one of the most crucial complications following endoscopic retrograde... more Background: Pancreatitis is one of the most crucial complications following endoscopic retrograde cholangiopancreatography (ERCP). The purpose of the current study was to investigate a potential procedure and patient-related risk factors for post-ERCP pancreatitis (PEP) in two groups: prophylactic pancreatic stent and rectal indomethacin.Methods: Two different prophylactic modalities were planned and complications were assessed based on defined inclusion criteria. In this study, the patients were evaluated for the procedure and patient-related risk factors in post-ERCP pancreatitis in the recipient groups of the prophylactic pancreatic stent or rectal indomethacin.Results: Pancreatitis was confirmed in 27 of all 175 selected patients after ERCP. By univariate analysis, two variables were significant with the development of PEP. Regarding the patient-related risk factors, unique subjects with common bile duct(CBD) dilated 10mm were more exposed to an increased chance of PEP (p=0. 015...

Research paper thumbnail of Naloxone Ameliorates Spatial Memory Deficits and Hyperthermia Induced by a Neurotoxic Methamphetamine Regimen in Male Rats

Galen Medical Journal

Background: Methamphetamine (METH) as a synthetic psychostimulant is being increasingly recognize... more Background: Methamphetamine (METH) as a synthetic psychostimulant is being increasingly recognized as a worldwide problem, which may induce memory impairment. On the other hand, it is well established that naloxone, an opiate antagonist, has some beneficial effects on learning and memory. The present research aimed at evaluating naloxone effects on spatial learning and memory impairment triggered by a neurotoxic regimen of METH in male rats. Materials and Methods: The animals received the subcutaneous (sc) regimen of METH (4×6 mg/kg at 2-h intervals), intraperitoneal (ip) naloxone (4×1 mg/kg at 2-h intervals), or normal saline at four events. The Nal-METH group of rats received four naloxone injections (1 mg/kg, ip) 30 min before each METH injection (6 mg/kg, sc) at 2-h intervals. Seven days later, they were evaluated for spatial learning and memory in the Morris Water Maze (MWM) task. Result: METH regimen induced hyperthermia, as well as a poor performance, in the acquisition and r...

Research paper thumbnail of Good Bacteria and Worms: As Promising Candidates for Inflammatory Bowel Disease

Journal of Mazandaran University of Medical Sciences, 2018

The gastrointestinal system harbors various microorganisms, known as gut microbiota. Increase in ... more The gastrointestinal system harbors various microorganisms, known as gut microbiota. Increase in prevalence of inflammatory bowel disease (IBD) in developed countries is associated with changes in the environment, such as decrease in incidence of parasitic infections, especially helminths, and changes in the intestinal flora. Probiotics are useful microbiota for human health. The parasitic worms (helminths) have evolved over the years with their host. Several studies have investigated the capability of helminths and probiotics to alter or control host immune responses in view of the fact that these organisms have immunomodulation effects. Given the contradictory results, further broad studies are needed to confirm the role of probiotics and helminths in the treatment of IBD. © 2018, Mazandaran University of Medical Sciences. All rights reserved.

Research paper thumbnail of Circulatory microRNAs: promising non-invasive prognostic and diagnostic biomarkers for parasitic infections

MicroRNAs (miRNAs) are a non-coding subclass of endogenous small regulatory RNAs, with about 18–2... more MicroRNAs (miRNAs) are a non-coding subclass of endogenous small regulatory RNAs, with about 18–25 nucleotides length which play a critical role in the regulation of gene expression at the post-transcriptional level in eukaryotes. Aberrant expression of miRNAs has the potential to become powerful non-invasive biomarkers in pathological diagnosis and prognosis of different disorders including infectious diseases. Parasite’s life cycle may require the ability to respond to environmental and developmental signals through miRNA-mediated gene expressions. Over the last years, thousands of miRNAs have been identified in the helminthic and protozoan parasites and many pieces of evidence have demonstrated the functional role of miRNAs in the parasites’ life cycle. Detection of these miRNAs in biofluids of infected hosts as prognostic and diagnostic biomarkers in infectious diseases is growing rapidly. In this review, we have highlighted altered expressions of host miRNAs, detected parasitic...

Research paper thumbnail of Relationship between ureB Sequence Diversity, Urease Activity and Genotypic Variations of Different Helicobacter pylori Strains in Patients with Gastric Disorders

Polish journal of microbiology, 2016

Association of the severity of Helicobacter pylori induced diseases with virulence entity of the ... more Association of the severity of Helicobacter pylori induced diseases with virulence entity of the colonized strains was proven in some studies. Urease has been demonstrated as a potent virulence factor for H. pylori. The main aim of this study was investigation of the relationships of ureB sequence diversity, urease activity and virulence genotypes of different H. pylori strains with histopathological changes of gastric tissue in infected patients suffering from different gastric disorders. Analysis of the virulence genotypes in the isolated strains indicated significant associations between the presence of severe active gastritis and cagA+ (P = 0.039) or cagA/iceA1 genotypes (P = 0.026), and intestinal metaplasia and vacA m1 (P = 0.008) or vacA s1/m2 (P = 0.001) genotypes. Our results showed a 2.4-fold increased risk of peptic ulcer (95% CI: 0.483-11.93), compared with gastritis, in the infected patients who had dupA positive strains; however this association was not statistically s...

Research paper thumbnail of Frequency of significant virulence genes in gastric biopsies of Helicobacter pylori-positive patients with gastritis

AMB Express

Helicobacter pylori is one of the most common bacteria affecting human societies worldwide, and i... more Helicobacter pylori is one of the most common bacteria affecting human societies worldwide, and is mainly associated with gastrointestinal complications due to different virulence factors. This study aimed to investigate some virulence genes of H. pylori in gastric biopsies of patients with gastritis in Sari city, North of Iran. Informed consent forms were obtained from the studied patients, and those who needed endoscopy were included in the study. To evaluate the prevalence of cagA, iceA1, iceA2, vacA, dupA, and oipA genes, gastric biopsies with positive or negative rapid urease test were collected from 50 patients (25 in each group) with gastro-duodenal diseases. The bacterial DNAs were extracted by a specific kit, and the presence of the genes was analyzed by PCR using specific primers. Eighteen (72%) biopsies from 25 H. pylori-positive samples were cagA-positive, while 17 (68%) biopsies contained the vacA gene, and 11 (44%) samples had both vacA and cagA genes. However, 16 (64%...

Research paper thumbnail of Effects of Estrogen and Progesterone on Behavioral Impairment and Neuronal Death in Ovariectomized Rats Induced by Methamphetamine

Journal of Mazandaran University of Medical Sciences, 2020

Background and purpose: Methamphetamine (METH) is one of the most powerful drugs that leads to ma... more Background and purpose: Methamphetamine (METH) is one of the most powerful drugs that leads to many cognitive and behavioral side effects such as anxiety. On the other hand, studies have shown that ovarian hormones such as estrogen and progesterone have neuroprotective effects on a wide range of cognitive and behavioral disorders. The aim of this study was to investigate the role of estrogen and progesterone on anxiety-like behaviors, body temperature, brain edema, and neuronal death induced by neurotoxic regimen of methamphetamine. Materials and methods: This study was performed in 48 ovariectomized rats divided into six groups including: control, METH (6mg / kg), vehicle (sesame oil), METH + estrogen (1mg / kg), METH + progesterone (8mg / kg), and METH + estrogen + progesterone. Body temperature and anxiety-like behaviors were investigated, then, the animals were killed and brain tissues were harvested to evaluate brain edema and neuronal death in hippocampal CA1. Results: Body te...

Research paper thumbnail of Effects of Clozapine and Risperidone Antipsychotic Drugs on the Expression of CACNA1C and Behavioral Changes in Rat ‘Ketamine Model of Schizophrenia

Research paper thumbnail of Procedure and Patient-Related Risk Factors for Post-ERCP Pancreatitis Associated with Prophylactic Pancreatic Stent and Rectal Indomethacin

Background: Pancreatitis is one of the most crucial complications following endoscopic retrograde... more Background: Pancreatitis is one of the most crucial complications following endoscopic retrograde cholangiopancreatography (ERCP). The purpose of the current study was to investigate a potential procedure and patient-related risk factors for post-ERCP pancreatitis (PEP) in two groups: prophylactic pancreatic stent and rectal indomethacin.Methods: Two different prophylactic modalities were planned and complications were assessed based on defined inclusion criteria. In this study, the patients were evaluated for the procedure and patient-related risk factors in post-ERCP pancreatitis in the recipient groups of the prophylactic pancreatic stent or rectal indomethacin.Results: Pancreatitis was confirmed in 27 of all 175 selected patients after ERCP. By univariate analysis, two variables were significant with the development of PEP. Regarding the patient-related risk factors, unique subjects with common bile duct(CBD) dilated 10mm were more exposed to an increased chance of PEP (p=0. 015...

Research paper thumbnail of Naloxone Ameliorates Spatial Memory Deficits and Hyperthermia Induced by a Neurotoxic Methamphetamine Regimen in Male Rats

Galen Medical Journal

Background: Methamphetamine (METH) as a synthetic psychostimulant is being increasingly recognize... more Background: Methamphetamine (METH) as a synthetic psychostimulant is being increasingly recognized as a worldwide problem, which may induce memory impairment. On the other hand, it is well established that naloxone, an opiate antagonist, has some beneficial effects on learning and memory. The present research aimed at evaluating naloxone effects on spatial learning and memory impairment triggered by a neurotoxic regimen of METH in male rats. Materials and Methods: The animals received the subcutaneous (sc) regimen of METH (4×6 mg/kg at 2-h intervals), intraperitoneal (ip) naloxone (4×1 mg/kg at 2-h intervals), or normal saline at four events. The Nal-METH group of rats received four naloxone injections (1 mg/kg, ip) 30 min before each METH injection (6 mg/kg, sc) at 2-h intervals. Seven days later, they were evaluated for spatial learning and memory in the Morris Water Maze (MWM) task. Result: METH regimen induced hyperthermia, as well as a poor performance, in the acquisition and r...

Research paper thumbnail of Good Bacteria and Worms: As Promising Candidates for Inflammatory Bowel Disease

Journal of Mazandaran University of Medical Sciences, 2018

The gastrointestinal system harbors various microorganisms, known as gut microbiota. Increase in ... more The gastrointestinal system harbors various microorganisms, known as gut microbiota. Increase in prevalence of inflammatory bowel disease (IBD) in developed countries is associated with changes in the environment, such as decrease in incidence of parasitic infections, especially helminths, and changes in the intestinal flora. Probiotics are useful microbiota for human health. The parasitic worms (helminths) have evolved over the years with their host. Several studies have investigated the capability of helminths and probiotics to alter or control host immune responses in view of the fact that these organisms have immunomodulation effects. Given the contradictory results, further broad studies are needed to confirm the role of probiotics and helminths in the treatment of IBD. © 2018, Mazandaran University of Medical Sciences. All rights reserved.

Research paper thumbnail of Circulatory microRNAs: promising non-invasive prognostic and diagnostic biomarkers for parasitic infections

MicroRNAs (miRNAs) are a non-coding subclass of endogenous small regulatory RNAs, with about 18–2... more MicroRNAs (miRNAs) are a non-coding subclass of endogenous small regulatory RNAs, with about 18–25 nucleotides length which play a critical role in the regulation of gene expression at the post-transcriptional level in eukaryotes. Aberrant expression of miRNAs has the potential to become powerful non-invasive biomarkers in pathological diagnosis and prognosis of different disorders including infectious diseases. Parasite’s life cycle may require the ability to respond to environmental and developmental signals through miRNA-mediated gene expressions. Over the last years, thousands of miRNAs have been identified in the helminthic and protozoan parasites and many pieces of evidence have demonstrated the functional role of miRNAs in the parasites’ life cycle. Detection of these miRNAs in biofluids of infected hosts as prognostic and diagnostic biomarkers in infectious diseases is growing rapidly. In this review, we have highlighted altered expressions of host miRNAs, detected parasitic...

Research paper thumbnail of Relationship between ureB Sequence Diversity, Urease Activity and Genotypic Variations of Different Helicobacter pylori Strains in Patients with Gastric Disorders

Polish journal of microbiology, 2016

Association of the severity of Helicobacter pylori induced diseases with virulence entity of the ... more Association of the severity of Helicobacter pylori induced diseases with virulence entity of the colonized strains was proven in some studies. Urease has been demonstrated as a potent virulence factor for H. pylori. The main aim of this study was investigation of the relationships of ureB sequence diversity, urease activity and virulence genotypes of different H. pylori strains with histopathological changes of gastric tissue in infected patients suffering from different gastric disorders. Analysis of the virulence genotypes in the isolated strains indicated significant associations between the presence of severe active gastritis and cagA+ (P = 0.039) or cagA/iceA1 genotypes (P = 0.026), and intestinal metaplasia and vacA m1 (P = 0.008) or vacA s1/m2 (P = 0.001) genotypes. Our results showed a 2.4-fold increased risk of peptic ulcer (95% CI: 0.483-11.93), compared with gastritis, in the infected patients who had dupA positive strains; however this association was not statistically s...