Dursun KISA | Gaziosmanpasa University (original) (raw)

Papers by Dursun KISA

Acta Botanica Croatica, Apr 1, 2019

The expression of stress related-genes against adverse environmental conditions has essential imp... more The expression of stress related-genes against adverse environmental conditions has essential importance for plants. This study, using RT-qPCR, determined the expression of P5CS and PCS genes to investigate their roles in the leaves of tomato plants grown under heavy metal conditions. The expression of the PCS1 gene is significantly induced under such conditions. Transcript expression of P5CS1, a gene responsible for proline synthesis, changed depending on heavy metal doses; treatments of Cu (20 and 50 ppm), Cd and Pb (10 and 20 ppm) remarkably increased P5CS1 expression. However, the P5CS1 gene expression at 10 ppm dose of Cu and 50 ppm doses of Pb and Cd was not significantly different from that in control plants. The metal-chelating potency of the extract of tomato leaves exposed to Pb and Cd was higher than that of untreated plants. The proline content as assessed in the leaves of stressed plants was significantly increased by applications of 10 and 20 ppm of Cd and Pb, and high doses of Cu. In addition, the results showed that the proline content had a positive correlation with the P5CS1 gene expression in tomato leaves under application of these tree heavy metals and that there was a positive relation between the PCS1 gene expression and metal-chelating ability of Cd-stressed plants.

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

Centaurea species has great potential as a traditional medicinal herb, and C. cadmea subsp. ponti... more Centaurea species has great potential as a traditional medicinal herb, and C. cadmea subsp. pontica collected from rocky slope crevices of Küre Mountain is endemic to the flora of Türkiye. In the present work, to reveal the plant's pharmacological importance, its potency to inhibit various medicinal enzymes was investigated, supported by molecular docking studies. The half-maximal inhibitory concentration (IC50) results for studied enzymes were quantified between 0.50-86.97 µg mLˉˡ, and the extract was efficient against HMG_CoA R, α-glucosidase, and α-amylase linked to diabetes and cholesterol. Nine phenolic compounds were identified in the C. cadmea subsp. pontica extract and the interactions of the most abundant phenolic compounds with the enzymes were examined with molecular docking studies. In conclusion, findings amassed from the present study inclined to support the opinion that C. cadmea subsp. pontica may be beneficial as an effective herb for formulating novel health-pr...

Chemistry & Biodiversity

Bioorganic & Medicinal Chemistry Letters

Phenolic compounds mainly benefit human health and have many biological activities. Their activit... more Phenolic compounds mainly benefit human health and have many biological activities. Their activities are related to their structure, which allows them to interact with enzymes. The inhibition potencies of synthesized polyphenolic compounds (3a and 3b) were investigated on cholinesterases, α‑Gly, and tyrosinase activities. The structures of 3a and 3b were determined based on spectral data (NMR, UV-vis, XRD pattern, SEM, and EDX). The compounds have effective inhibitory potential with IC50 value between 2.25±0.35 - 5.66±0.75 µM and Ki values 2.95±0.37 - 14.86±4.99 µM for AChE, BChE, and tyrosinase. It was determined the synthesized compounds have biological activities by the MIC and cytotoxicity tests, and they have IC50 values of 16.15 µg/mL and 12.16 µg/mL for the PC-3 cell line, respectively. According to the calculated molecular docking results, these compounds showed the highest binding energy against AChE and tyrosinase enzymes (-11.3 and -10.4 Kcal/mol, respectively). The compounds have synthetic accessibility scores of 2.75 and 4.55 based on the drug-likeness properties.

International Eurasian Conference on Biological and Chemical Sciences, Jul 1, 2019

Inhibition properties of some coordination compounds containing dicyanidogylene were investigated... more Inhibition properties of some coordination compounds containing dicyanidogylene were investigated on human carbonic anhydrase I (CA I, E.C. 4.2.1.1). Carbonic anhydrase, a multifunctional enzyme, catalyzes reversibly a crucial reaction between carbon dioxide and water, leading to the formation of protons (H+) and bicarbonate (HCO3). CA inhibitors (CAIs) have clinical importance because they are used in the treatment of various diseases such as antiglaucoma, anticonvulsant and diuretics. Coordination complexes have remarkable biological activities such as antibacterial, antifungal and antitumor activities, and as therapeutic compounds due to their pharmaceutical properties. Metal complexes have effects on the inhibition of enzymes that play a role in biochemical reactions, which are important reactions for human life quality. In the present study, we investigate inhibitory effect of two coordination compounds ([Ni(bishydeten)2Ag(CN)2][Ag(CN)2].H2O and [Ni(edbea)Ag3(CN)5]) synthesized by our project team on hCA I and enzyme activity was measured by spectrophotometrically. Inhibition constant (Ki) were calculated from drawn Lineweaver Burk charts. These complexes exhibited effectively inhibitory effects on hCA I and Ki values were found as 635.30 nM and 184.01 nM, respectively. The IC50 values of the complexes against hCA I were 350.01 µM and 466.02 µM, respectively. Inhibition type of the complexes is noncompetitive against to hCA I enzyme activity. In conclusion, it has been indicated that novel coordination complexes have in vitro inhibitory effect on hCA. In the future, the inhibitory effect of these compounds can be observed in animal experiments for beneficial health effects and these data may lead to designing potent novel inhibitors

Arabian Journal of Chemistry, 2022

The accumulation of heavy metals in the agricultural environments causes the oxidative stress in ... more The accumulation of heavy metals in the agricultural environments causes the oxidative stress in leading physiological and biochemical alterations in the plants. Regulation of fatty acids is considered as an adaptive mechanism for plants exposed to oxidative damages. In the present study, we investigated the changes of fatty acid composition with GC in the leaves of tomato subjected to increasing doses of heavy metals. The exposures of heavy metals changed the fatty acid compositions and α-linoleic acid, palmitic acid and linoleic acid were the main fatty acids in respect to percentage, respectively. The percentages of arachidic acid, behenic acid, lignoceric acid and docosahexaenoic acid clearly increased in leaves of tomato. The level of lionoleic acid and palmitoleic acid significantly reduced in all application of heavy metals compared to control plants. The content of stearic acid and oleic acid methyl ester changed depending on heavy metal types and doses while the quantity of...

Bu calismada, de-etiyole fasulye fidelerinde antioksidan enzim aktiviteleri, hidrojen peroksit, m... more Bu calismada, de-etiyole fasulye fidelerinde antioksidan enzim aktiviteleri, hidrojen peroksit, malondialdehit ve total fenolik madde miktarlari arastirilmistir. De-etiyole fasulye yapraklarinda katalaz (CAT), peroksidaz (POD) ve askorbat peroksidaz (APX) aktiviteleri kontrol (isik) grubuyla karsilastirildiginda kismi olarak yuksek oldugu gorulmustur. De-etiyole fasulye bitkisinde lipid peroksidasyonu kontrol grubundan onemli oranda yuksek olmasina ragmen hidrojen peroksit miktarinda onemli bir fark yoktur. Total fenolik bilesikler kontrol ve de-etiyole bitkilerde yuksek oranda gozlenirken etiyole fidelerde belirlenememistir. Karanlik-isik gecislerinde oksidatif hasar azalirken total fenolik bilesik miktarinda artis meydana gelmistir.

Extracellular matrix (ECM) consist of laminin, collagen, elastin, fibroblasts, and glycosaminogly... more Extracellular matrix (ECM) consist of laminin, collagen, elastin, fibroblasts, and glycosaminoglycans. Its main role maintain the integrity and strength of organs as well as acts as a primary barrier to prevent the spread of the tumor cells because is a major component of the cellular microenvironment. The degradation of ECM involves different types of matrix metalloproteases (MMPs) such as collagenase and elastase. MMPs play a central role in major stages of tumor progression so novel potential inhibitors should be developed to prevent their protease activities. Coordination complexes have been used in medicine for their therapeutic properties. In the present study, we aim to investigate in vitro inhibitory effect of [Ni(edbea)Ag3(CN)5] against to collagenase and elastase activities by spectrophotometrically. Inhibition constant (Ki) of these enzymes was obtained from drawn Lineweaver Burk graphs. The complex containing silver exhibited effectively inhibitory effects on collagenase and elastase. Ki constants against to collagenase and elastase was found as 16.73+1.07 µM and 42.81 ± 9.62 µM, respectively. The IC50 value of the novel compound against to these enzymes was 10.66 µM and 49.5 µM, respectively. Inhibition type of the compound was noncompetitive against to collagenase and elastase activity. In conclusion, it has been indicated that novel dicyanidogylene compound have in vitro inhibitory effect on collagenase and elastase. In the future, the inhibitory effect of the molecule on ECM-degrading enzymes can be investigated in cell cultures and animal experiments for beneficial effects and the result may lead to designing potent new inhibitors.

Heavy metals are one of the abiotic stress factor for plants, they effect the plant life cycles b... more Heavy metals are one of the abiotic stress factor for plants, they effect the plant life cycles binding to sulfhydryl groups in amino acids and proteins. The presence of heavy metal in plant tissues changes the plant’s molecular, biochemical and physiological responses such as variations in gene expression, enzyme activities and amino acid content. This study aimed to determine the expression of P5CS and PCS genes to investigate their roles in the leaves of tomato plants subjected to increasing doses of heavy metals. The cultivars of tomato (Solanum lycopersicum cv.) were cultivated in plastic pots containing peat and garden soil (1:1) under greenhouse conditions. Three weeks after the plantings, the tomato plants were exposed to 10, 20 and 50 ppm doses of CdCI2, CuSO4 and Pb(NO3)2 salts. The leaves of tomato were harvested for sampling two weeks after the treatments and total RNA was extracted by using the Plant RNA Mini-Preps Kit. The first-strand cDNA was synthesized from the isolated RNA; the expression of the selected genes was detected by using RTqPCR with SYBR Green RT-PCR kit. Also, free proline content and metal-chelating ability was identified spectrophotometrically. The application of heavy metals significantly increased the expression of PCS1 gene in the leaves of tomato compared to control plants. The most evident expressions were observed in plants given the high doses of heavy metals. Transcript expression of P5CS1 changed depending on heavy metal doses; treatments of Cu (20 and 50 ppm), Cd and Pb (10 and 20 ppm) remarkably increased P5CS1 expression. The metal-chelating potency of the extract of tomato leaves exposed to Pb and Cd was higher than that of untreated plants. The proline content in the leaf extract significantly increased with the addition of 10 and 20 ppm of Pb and Cd, but significantly decreased at high doses (50 ppm) (p<0.05). The metal-chelating activity was higher in intervention plants cultivated in Pb and Cd-containing soils than in control plants (p<0.05). The results showed that the proline content had a positive correlation with P5CS1 transcript expression when the tomato plant is subjected to the three heavy metals. In addition, there was a positive relation between the PCS gene expression and metalchelating ability of Cd-stressed plants. In this context, the gene expressions of PCS1 and P5CS1 related with the PCs and proline content, respectively were correlated in the leaves of tomato under heavy metal stress. To further define and elucidate the responses of plants under stress conditions, the expression of genes and their corresponding enzymes associated with PCs and proline metabolisms, such as P5CR, P5CDH, PDH, POX, ProT, ɣ- ECS, and PC synthase, should be studied together to reveal the depth of responses in tomato subjected to various environmental conditions

Bu calismada, karanlik ortamda cimlendirilerek buyutulen fasulye fidelerine yedi gun isik veriler... more Bu calismada, karanlik ortamda cimlendirilerek buyutulen fasulye fidelerine yedi gun isik verilerek hasat edilmistir. De-etiyole fasulye fidelerinin yaprak ve koklerinde yag asidi icerigi, hidrojen peroksit miktari ve lipid peroksidasyonu arastirilmistir. Fasulye fidelerinin yaprak ve koklerinin lipid bilesimi, hidrojen peroksit miktari ve lipid peroksidasyonu farklilik gostermistir. Karanlik-isik gecislerinde fasulye fidelerinin yaprak ve koklerinde coklu doymus yag asidi miktarinda azalma gorulmustur. Yapraklarin hidrojen peroksit icerigi ve lipid peroksidasyonu koklerden daha fazladir. Isigin etkisiyle yapraklarda hidrojen peroksit ve lipid peroksidasyonu azalmistir. Karanlik-isik gecislerinde yapraklarda lipit peroksidasyonunun azalmasi coklu doymamis yag asit miktarindaki azalma ve antioksidan enzim ve bilesiklerin sentezlerindeki artistan kaynaklanabilir.

Heavy metals have restricted the plant regular life cycles affecting the plant primer and seconde... more Heavy metals have restricted the plant regular life cycles affecting the plant primer and seconder metabolites by biochemical and physiological pathways. Phenolic compounds considered as products of metabolic alterations have been synthesized in various numbers and typical characteristic of plants. In this study, we aimed to investigate the variations of phenolic compounds with HPLC in leaves of tomato exposed to heavy metals. The applications of Cu, Cd and Pb significantly reduced the total phenolic content, levels of caffeic, chlorogenic and vanillic acids in all treated groups except for 50 and 20 ppm of Pb for total phenolics and vanillic acid, respectively. The level of benzoic acid is generally decreased by the application of heavy metals except for Cu at 50 ppm doses. Rutin is the most abundant phenolic compound in term of quantity among to analyzed phenolics and its content decreased depending on the heavy metal doses except for 10 ppm doses of Cd. The responses of tomato un...

Bioorganic Chemistry, 2020

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Journal of Plant Research, 2016

Heavy metals such as Cd are considered to be the most important pollutants in soil contamination.... more Heavy metals such as Cd are considered to be the most important pollutants in soil contamination. Cd is a non-essential element adversely affecting plant growth and development, and it has caused some physiological and molecular changes. Metallothioneins (MTs) are low molecular weight, cysteine-rich, and metal binding proteins. In this study, we aimed to evaluate the MT gene expression levels and minerals uptake in the tissues of Solanum lycopersicum exposed to Cd. The transcriptional expression of the MT genes was determined by real-time quantitative PCR. The MT genes were regulated by the Cd and the mineral elements uptake changed tissue type and applied doses. The MT1 and MT2 transcript levels increased in the roots, the leaves and the fruits of the tomato. The MT3 and MT4 transcript pattern changed according to the tissue types. The Cd treatment on the growth medium increased the Mg, Ca, and Fe content in both the leaves and fruits of the tomato. However, the Cd affected the mineral levels in the roots depending on the mineral types and doses. Also, the Cd content increased in the roots, the leaves, and the fruits of the tomato, respectively. The results presented in this study show that Cd has synergistic and/or antagonistic effects on minerals depending on the tissue types. These results indicate that the MT1 and MT2 expression pattern increased together with the Mg, Ca, and Fe content in both the leaves and the fruits of the tomato.

Applied Biological Chemistry, 2016

Heavy metal toxicity is one of the major abiotic stresses caused by physiological and biochemical... more Heavy metal toxicity is one of the major abiotic stresses caused by physiological and biochemical changes. Plants have evolved various phytochemical defense mechanisms to cope with this abiotic stress conditions. Phenolic compounds are one of the stress responses and have multiple roles in respect to adaptation of plants to the environment. In the present study, we aimed to evaluate the differential accumulation of various phenolics with HPLC in the leaves of corn exposed to increasing heavy metal doses in the plant growth medium. The application of Cd, Cu, and Pb increased the total phenolics in all treatments compared to control groups. Chlorogenic acid and rutin were the main phenolic compounds in respect to quantifying. However, the contents of caffeic acid, ferulic acid, and vanillic acid were comparatively lower than chlorogenic acid and rutin in all samples. The content of chlorogenic acid significantly increased and rutin slightly increased in the treatment of the heavy metals. The levels of caffeic acid and ferulic acid significantly decreased in all exposures of heavy metals compared to control groups. The content of vanillic acid changed according to heavy metal types and doses in the leaves of corn, and the low doses of Pb and Cd increased the level of vanillic acid. We show that there is a positive correlation with the total phenolic content and chlorogenic acid when the corn is exposed to Pb. Moreover, there are negative correlations between total phenolic compound and caffeic acid, ferulic acid in the application of Cu and Cd.

Sakarya University Journal of Science

The ulcer is a vital disease that unfavorably affects human health globally. Helicobacter pylori,... more The ulcer is a vital disease that unfavorably affects human health globally. Helicobacter pylori, a reason forhave an antibacterial effect against ulcers and some stomach disorders, live on using the urease enzyme and induces the condition to occur. Metal coordination complexes (MCC) are used in numerous industrial areas and the health field. Cyanide bridged metal complexes are also applied in several places. In the current study, antibacterial characteristic of (C1), [Ni(bishydeten)2Ag(CN)2][Ag(CN)2].H2O), and (C2), ([Ni(hydeten)2Ag(CN)2][Ag(CN)2], MCC were analyzed by disk diffusion and broth dilution broth and urease enzyme inhibition assays were performed. As a result of both antibacterial tests, C1 and C2 were observed to provide favorable effects. The synthesized compounds have effective inhibitory potential with IC50 value between 26.65±1.21 and 12.37±0.87 µM for urease.

Applied Organometallic Chemistry, 2021

The ability to design nanowires (NW) in powder technology provides a great advantage for many app... more The ability to design nanowires (NW) in powder technology provides a great advantage for many applications in a variety of fields ranging from medicine to materials science. In this study, nanoparticles were synthesized simultaneously by using an aqueous two‐phase system, which was an environmentally friendly method since the reducing agent that was not spent in the reaction could be effectively recycled and reused. AgNWs (silver nanowires) with numerous high widths and dyes were easily synthesized between two phases using the 5,8‐dibromo‐2‐(butylamino)naphthalene‐1,4‐dione, a 2‐substituted naphthoquinone. Naphthoquinones are known as reducing agents in the production of metal nanoparticles. Synthesized NWs have an average diameter of 90 nm and an average length of 6 μm. The nanowires produced had a round cross section. Characterization of nanowires and nanoparticles was done with UV‐vis, XRD pattern, SEM, and EDX. As a result of characterization, it showed two relatively sharp absorbances in the UV‐vis spectrum at a wavelength of ~358 and 432 nm. In the XRD analysis, five basic peaks attributed to Ag nanoparticles were observed. When SEM images were analyzed, it was determined that Ag nanowires generally have an average diameter of 90 nm and an average length of 6 μm. Anticancer activity test was performed using the MTT test in the HeLa and HT‐29 cell lines. IC50 value was calculated as 11.54 and 16.07 μg ml, and AgNWs showed anticancer properties at high concentration. Evaluating the influence of AgNWs for the first time, this study has indicated that AgNWs exhibit inhibitory properties on the collagenase enzyme. It was observed that the IC50 rate for the enzyme activity was 346.5 mM (r²: 0.955), and Ki value was 41.09 mM.