JUAN BOSSIO | Butex - Academia.edu (original) (raw)
Papers by JUAN BOSSIO
Molecular Microbiology, 1994
We have identified two novel Salmonella typhimurium genes, invF and invG, which are required for ... more We have identified two novel Salmonella typhimurium genes, invF and invG, which are required for the efficient entry of these organisms into cultured epithelial cells. invF and invG are located immediately upstream of invE, a previously identified gene also required for Salmonella entry. Non-polar mutations in these genes rendered S. typhimurium severely deficient for entry into cultured epithelial cells. The nucleotide sequences of invF and invG indicated that these genes encode polypeptides with predicted molecular weights of 24,373 and 62,275, respectively. Proteins of similar sizes were observed when invF and invG were expressed in a bacteriophage T7 RNA polymerase-based expression system. Comparison of the predicted sequence of InvF with translated sequences in the existing databases indicated that this protein is homologous to members of the AraC family of prokaryotic transcription regulators. However, mutations in invF did not significantly affect the expression of other members of the inv locus. InvG was found to be homologous to members of the PulD family of specialized translocases. This homology suggests that InvG may be necessary for the export of invasion-related determinants or involved in the assembly of a supramolecular structure that promotes entry.
Molecular and Biochemical Parasitology, 1990
Protein N-glycosylation is initiated in trypanosomatid protozoa by the transfer of nonglucosylate... more Protein N-glycosylation is initiated in trypanosomatid protozoa by the transfer of nonglucosylated oligosaccharides (Man6GlcNAc2, ManT-GlcNAc2 or MangGlcNAc2, depending on the species) from dolichol-P-P derivatives to asparagine residues in polypeptide chains [ 1 ]. In this respect, these protozoa differ from all other wild type eucaryotic cells in which Glc3MangGlcNAc2 is transferred [2]. In all eukaryotes the oligosaccharities are processed as protein-linked glycans. We have previously reported that processing in some monogenetic trypanosomatids (Crithidia fasciculata, Crithidia harmosa, Leptomonas samueli, and Herpetomonas samuelpessoai) involved addition of galactose units to highmannose-type compounds [3-5]. The structure of the oligosaccharides occurring in the first three microorganisms was described [3,4]. The galactose residues were in the furanose configuration and linked directly to the non-reducing terminal
International Journal of Environmental Analytical Chemistry, 2011
... 1996. Toxic Microcystis , Edited by: Watanabe, MF, Haradmka, K, Carmichael, WW and Fujiki, H.... more ... 1996. Toxic Microcystis , Edited by: Watanabe, MF, Haradmka, K, Carmichael, WW and Fujiki, H. Boca Raton, FL: CRC Press. View all references,22. Haider, S, Naithani, V, Viswanathan, PN and Kakkar, P. 2003. ... De Leon, L and Yunes, JS. 2001. Environ. Toxicol. ...
Biochemical and Biophysical Research Communications, 1986
Mitochondrial DNA, 2000
ABSTRACT A DNA sequence composed of 1281 nucleotides (nt) consisting of a single open reading fra... more ABSTRACT A DNA sequence composed of 1281 nucleotides (nt) consisting of a single open reading frame (ORF) encoding a putative 20S proteasome beta1-type subunit was isolated from clones derived from genomic libraries constructed from the KSU-1 isolate of Cryptosporidium parvum. Southern blot analysis suggested that the sequenced DNA exists in the C. parvum genome as a single copy; transcription was verified through reverse transcription-polymerase chain reaction (RT-PCR) performed on total RNA isolated from C. parvum sporozoites. The predicted protein consists of 210 amino acids (aa), contains characteristic amino acids common to all proteasomal subunits, and shares stronger similarity to the beta1-type subunit of yeast than to other types of beta-subunits.
… Public Access to …, 2011
FABICIB
La malnutrición infantil es un estado donde existe una insuficiencia o exceso de nutrientes que c... more La malnutrición infantil es un estado donde existe una insuficiencia o exceso de nutrientes que conlleva a diversas patologías. La edad escolar es un período crítico donde deben realizarse exámenes periódicos del estado nutricional. En este estudio se ponderó la prevalencia de malnutrición en escolares de 1º y 4º grado, en 9 escuelas de la ciudad de Santa Fe. Se tomaron medidas antropométricas de peso y talla. Se utilizó el indicador Índice de Masa Corporal para la Edad (IMC/E), para determinar el estado nutricional. Se halló una alta prevalencia de malnutrición por exceso: 30,11% de sobrepeso y 23,43% de obesidad, en un total de 734 niños evaluados. El bajo peso sólo constituyó un 0,41% de la muestra. Hubo mayor proporción de malnutrición por exceso entre los varones y los niños de 4º grado. Las cifras obtenidas constituyen un punto de partida para la realización de intervenciones que busquen prevenir y mitigar esta situación.
International Journal of Environmental Analytical Chemistry, 2011
Microcystis is one of the most common bloom-forming cyanobacteria genera in diverse ecosystems. M... more Microcystis is one of the most common bloom-forming cyanobacteria genera in diverse ecosystems. More than 80% of its strains are toxic, mainly due to their ability to produce metabolites known as microcystins (MC). Here we report on a M. aeruginosa bloom that appeared in the summer of 2001 at a site of the Río de la Plata, within the city of Buenos Aires. The symptoms in mice indicated that the bloom was hepatotoxic. LC-PDA analysis revealed a similar high concentration (0.9–1 mg g–1 d w) of just one MC in the bloom biomass during the 3-month study period. During this period most of the MC (ca. 98 %) was found intracellularly, and the content remained almost the same. The molecular mass of the bloom MC was 1036 Da, as deduced from LC-ESI-MS data. Fragment ion analysis by LC-IT-MS-MS allowed identifying 6 out of the 7 amino acids, as well as their position in the molecule. The molecular mass of the unidentified amino acid residue was 155 Da. According to the data obtained, the MC under study was MC-XR, X representing the unidentified amino acid. This is the first report both on the characterisation of MCs in an urban site of the Río de la Plata waters, and on an Argentinean bloom exhibiting only one MC variant.
Molecular Microbiology, 1994
We have identified two novel Salmonella typhimurium genes, invF and invG, which are required for ... more We have identified two novel Salmonella typhimurium genes, invF and invG, which are required for the efficient entry of these organisms into cultured epithelial cells. invF and invG are located immediately upstream of invE, a previously identified gene also required for Salmonella entry. Non-polar mutations in these genes rendered S. typhimurium severely deficient for entry into cultured epithelial cells. The nucleotide sequences of invF and invG indicated that these genes encode polypeptides with predicted molecular weights of 24373 and 62275, respectively. Proteins of similar sizes were observed when invF and invG were expressed in a bacteriophage T7 RNA polymerase-based expression system. Comparison of the predicted sequence of InvF with translated sequences in the existing databases indicated that this protein is homologous to members of the AraC family of prokaryotic transcription regulators. However, mutations in invF did not significantly affect the expression of other members of the inv locus. InvG was found to be homologous to members of the PuID family of specialized translocases. This homology suggests that InvG may be necessary for the export of invasion-related determinants or involved in the assembly of a supramolecular structure that promotes entry.
Molecular Microbiology, 1993
Our laboratories have independently identified a gene in Salmonella choleraesuis and Salmonella t... more Our laboratories have independently identified a gene in Salmonella choleraesuis and Salmonella typhimurium that is necessary for efficient adherence and entry of these organisms into cultured epithelial cells. Introduction of a mutated gene into several Salmonella strains belonging to different serotypes rendered these organisms deficient for adherence and invasion of cultured cells. This effect was most pronounced in the host-adapted serotypes Salmonella gallinarum, S. choleraesuis, and Salmonella typhi. The nucleotide sequence of this gene, which we have termed invH, encodes a predicted 147-amino-acid polypeptide containing a signal sequence. The InvH predicted polypeptide is highly conserved in S. typhimurium and S. choleraesuis, differing at only three residues. The invH gene was expressed in Escherichia coli using a T7 RNA polymerase expression system and a polypeptide of ∼16000 molecular weight was observed, in agreement with the predicted size of its gene product. Upon fractionation, the expressed polypeptide was localized in the bacterial membrane fraction. Southern and colony hybridization analyses indicated that the invH gene is present in all Salmonella strains tested (91 strains belonging to 37 serotypes) with the exception of strains of Salmonella arizonae. No homologous sequences were detected in Yersinia, Shigella, Proteus, and several strains of enteroinvasive and enteropathogenic E. coli. Downstream from the S. choleraesuis (but not S. typhimurium) invH gene, a region with extensive homology to the insertion sequence IS3 was detected.
Biochemical and Biophysical Research Communications, 1986
Molecular and Biochemical Parasitology, 1990
Protein N-glycosylation is initiated in trypanosomatid protozoa by the transfer of nonglucosylate... more Protein N-glycosylation is initiated in trypanosomatid protozoa by the transfer of nonglucosylated oligosaccharides (Man6GlcNAc2, ManT-GlcNAc2 or MangGlcNAc2, depending on the species) from dolichol-P-P derivatives to asparagine residues in polypeptide chains . In this respect, these protozoa differ from all other wild type eucaryotic cells in which Glc3MangGlcNAc2 is transferred . In all eukaryotes the oligosaccharities are processed as protein-linked glycans. We have previously reported that processing in some monogenetic trypanosomatids (Crithidia fasciculata, Crithidia harmosa, Leptomonas samueli, and Herpetomonas samuelpessoai) involved addition of galactose units to highmannose-type compounds . The structure of the oligosaccharides occurring in the first three microorganisms was described . The galactose residues were in the furanose configuration and linked directly to the non-reducing terminal
Molecular Microbiology, 1994
We have identified two novel Salmonella typhimurium genes, invF and invG, which are required for ... more We have identified two novel Salmonella typhimurium genes, invF and invG, which are required for the efficient entry of these organisms into cultured epithelial cells. invF and invG are located immediately upstream of invE, a previously identified gene also required for Salmonella entry. Non-polar mutations in these genes rendered S. typhimurium severely deficient for entry into cultured epithelial cells. The nucleotide sequences of invF and invG indicated that these genes encode polypeptides with predicted molecular weights of 24,373 and 62,275, respectively. Proteins of similar sizes were observed when invF and invG were expressed in a bacteriophage T7 RNA polymerase-based expression system. Comparison of the predicted sequence of InvF with translated sequences in the existing databases indicated that this protein is homologous to members of the AraC family of prokaryotic transcription regulators. However, mutations in invF did not significantly affect the expression of other members of the inv locus. InvG was found to be homologous to members of the PulD family of specialized translocases. This homology suggests that InvG may be necessary for the export of invasion-related determinants or involved in the assembly of a supramolecular structure that promotes entry.
Molecular and Biochemical Parasitology, 1990
Protein N-glycosylation is initiated in trypanosomatid protozoa by the transfer of nonglucosylate... more Protein N-glycosylation is initiated in trypanosomatid protozoa by the transfer of nonglucosylated oligosaccharides (Man6GlcNAc2, ManT-GlcNAc2 or MangGlcNAc2, depending on the species) from dolichol-P-P derivatives to asparagine residues in polypeptide chains [ 1 ]. In this respect, these protozoa differ from all other wild type eucaryotic cells in which Glc3MangGlcNAc2 is transferred [2]. In all eukaryotes the oligosaccharities are processed as protein-linked glycans. We have previously reported that processing in some monogenetic trypanosomatids (Crithidia fasciculata, Crithidia harmosa, Leptomonas samueli, and Herpetomonas samuelpessoai) involved addition of galactose units to highmannose-type compounds [3-5]. The structure of the oligosaccharides occurring in the first three microorganisms was described [3,4]. The galactose residues were in the furanose configuration and linked directly to the non-reducing terminal
International Journal of Environmental Analytical Chemistry, 2011
... 1996. Toxic Microcystis , Edited by: Watanabe, MF, Haradmka, K, Carmichael, WW and Fujiki, H.... more ... 1996. Toxic Microcystis , Edited by: Watanabe, MF, Haradmka, K, Carmichael, WW and Fujiki, H. Boca Raton, FL: CRC Press. View all references,22. Haider, S, Naithani, V, Viswanathan, PN and Kakkar, P. 2003. ... De Leon, L and Yunes, JS. 2001. Environ. Toxicol. ...
Biochemical and Biophysical Research Communications, 1986
Mitochondrial DNA, 2000
ABSTRACT A DNA sequence composed of 1281 nucleotides (nt) consisting of a single open reading fra... more ABSTRACT A DNA sequence composed of 1281 nucleotides (nt) consisting of a single open reading frame (ORF) encoding a putative 20S proteasome beta1-type subunit was isolated from clones derived from genomic libraries constructed from the KSU-1 isolate of Cryptosporidium parvum. Southern blot analysis suggested that the sequenced DNA exists in the C. parvum genome as a single copy; transcription was verified through reverse transcription-polymerase chain reaction (RT-PCR) performed on total RNA isolated from C. parvum sporozoites. The predicted protein consists of 210 amino acids (aa), contains characteristic amino acids common to all proteasomal subunits, and shares stronger similarity to the beta1-type subunit of yeast than to other types of beta-subunits.
… Public Access to …, 2011
FABICIB
La malnutrición infantil es un estado donde existe una insuficiencia o exceso de nutrientes que c... more La malnutrición infantil es un estado donde existe una insuficiencia o exceso de nutrientes que conlleva a diversas patologías. La edad escolar es un período crítico donde deben realizarse exámenes periódicos del estado nutricional. En este estudio se ponderó la prevalencia de malnutrición en escolares de 1º y 4º grado, en 9 escuelas de la ciudad de Santa Fe. Se tomaron medidas antropométricas de peso y talla. Se utilizó el indicador Índice de Masa Corporal para la Edad (IMC/E), para determinar el estado nutricional. Se halló una alta prevalencia de malnutrición por exceso: 30,11% de sobrepeso y 23,43% de obesidad, en un total de 734 niños evaluados. El bajo peso sólo constituyó un 0,41% de la muestra. Hubo mayor proporción de malnutrición por exceso entre los varones y los niños de 4º grado. Las cifras obtenidas constituyen un punto de partida para la realización de intervenciones que busquen prevenir y mitigar esta situación.
International Journal of Environmental Analytical Chemistry, 2011
Microcystis is one of the most common bloom-forming cyanobacteria genera in diverse ecosystems. M... more Microcystis is one of the most common bloom-forming cyanobacteria genera in diverse ecosystems. More than 80% of its strains are toxic, mainly due to their ability to produce metabolites known as microcystins (MC). Here we report on a M. aeruginosa bloom that appeared in the summer of 2001 at a site of the Río de la Plata, within the city of Buenos Aires. The symptoms in mice indicated that the bloom was hepatotoxic. LC-PDA analysis revealed a similar high concentration (0.9–1 mg g–1 d w) of just one MC in the bloom biomass during the 3-month study period. During this period most of the MC (ca. 98 %) was found intracellularly, and the content remained almost the same. The molecular mass of the bloom MC was 1036 Da, as deduced from LC-ESI-MS data. Fragment ion analysis by LC-IT-MS-MS allowed identifying 6 out of the 7 amino acids, as well as their position in the molecule. The molecular mass of the unidentified amino acid residue was 155 Da. According to the data obtained, the MC under study was MC-XR, X representing the unidentified amino acid. This is the first report both on the characterisation of MCs in an urban site of the Río de la Plata waters, and on an Argentinean bloom exhibiting only one MC variant.
Molecular Microbiology, 1994
We have identified two novel Salmonella typhimurium genes, invF and invG, which are required for ... more We have identified two novel Salmonella typhimurium genes, invF and invG, which are required for the efficient entry of these organisms into cultured epithelial cells. invF and invG are located immediately upstream of invE, a previously identified gene also required for Salmonella entry. Non-polar mutations in these genes rendered S. typhimurium severely deficient for entry into cultured epithelial cells. The nucleotide sequences of invF and invG indicated that these genes encode polypeptides with predicted molecular weights of 24373 and 62275, respectively. Proteins of similar sizes were observed when invF and invG were expressed in a bacteriophage T7 RNA polymerase-based expression system. Comparison of the predicted sequence of InvF with translated sequences in the existing databases indicated that this protein is homologous to members of the AraC family of prokaryotic transcription regulators. However, mutations in invF did not significantly affect the expression of other members of the inv locus. InvG was found to be homologous to members of the PuID family of specialized translocases. This homology suggests that InvG may be necessary for the export of invasion-related determinants or involved in the assembly of a supramolecular structure that promotes entry.
Molecular Microbiology, 1993
Our laboratories have independently identified a gene in Salmonella choleraesuis and Salmonella t... more Our laboratories have independently identified a gene in Salmonella choleraesuis and Salmonella typhimurium that is necessary for efficient adherence and entry of these organisms into cultured epithelial cells. Introduction of a mutated gene into several Salmonella strains belonging to different serotypes rendered these organisms deficient for adherence and invasion of cultured cells. This effect was most pronounced in the host-adapted serotypes Salmonella gallinarum, S. choleraesuis, and Salmonella typhi. The nucleotide sequence of this gene, which we have termed invH, encodes a predicted 147-amino-acid polypeptide containing a signal sequence. The InvH predicted polypeptide is highly conserved in S. typhimurium and S. choleraesuis, differing at only three residues. The invH gene was expressed in Escherichia coli using a T7 RNA polymerase expression system and a polypeptide of ∼16000 molecular weight was observed, in agreement with the predicted size of its gene product. Upon fractionation, the expressed polypeptide was localized in the bacterial membrane fraction. Southern and colony hybridization analyses indicated that the invH gene is present in all Salmonella strains tested (91 strains belonging to 37 serotypes) with the exception of strains of Salmonella arizonae. No homologous sequences were detected in Yersinia, Shigella, Proteus, and several strains of enteroinvasive and enteropathogenic E. coli. Downstream from the S. choleraesuis (but not S. typhimurium) invH gene, a region with extensive homology to the insertion sequence IS3 was detected.
Biochemical and Biophysical Research Communications, 1986
Molecular and Biochemical Parasitology, 1990
Protein N-glycosylation is initiated in trypanosomatid protozoa by the transfer of nonglucosylate... more Protein N-glycosylation is initiated in trypanosomatid protozoa by the transfer of nonglucosylated oligosaccharides (Man6GlcNAc2, ManT-GlcNAc2 or MangGlcNAc2, depending on the species) from dolichol-P-P derivatives to asparagine residues in polypeptide chains . In this respect, these protozoa differ from all other wild type eucaryotic cells in which Glc3MangGlcNAc2 is transferred . In all eukaryotes the oligosaccharities are processed as protein-linked glycans. We have previously reported that processing in some monogenetic trypanosomatids (Crithidia fasciculata, Crithidia harmosa, Leptomonas samueli, and Herpetomonas samuelpessoai) involved addition of galactose units to highmannose-type compounds . The structure of the oligosaccharides occurring in the first three microorganisms was described . The galactose residues were in the furanose configuration and linked directly to the non-reducing terminal