María López-jurado | University of Granada (original) (raw)

Papers by María López-jurado

RICYDE. Revista internacional de ciencias del deporte, 2015

Journal of agricultural and food chemistry, Jan 20, 2005

The effect of the removal of alpha-galactosides from Lupinus albus L. var. multolupa on the chemi... more The effect of the removal of alpha-galactosides from Lupinus albus L. var. multolupa on the chemical composition of the prepared flour and the dialyzability of N, total P, Ca, Mg, Fe, Zn, and Mn was studied. The extraction process caused a significant increase in total and insoluble nitrogen contents and decreased the amount of soluble protein nitrogen. However, neither these changes nor treatment with phytase seemed to considerably affect in vitro protein digestibility. Except for Ca and Cu, total mineral contents were significantly reduced by the extraction process. The process also caused a significant reduction in the dialyzability of all the minerals studied except P. The decrease in mineral dialyzability was partially counteracted by phytase treatment in amounts of 250-500 phytase units/kg of lupin flour. In the case of Fe, mineral dialyzability did not differ significantly between the two lupin flours studied with treatment with 500 phytase units/kg. Zinc dialyzability was th...

The Journal of nutrition, 1999

Patients with peripheral vascular disease (Fontaine stage II) are characterized by ischemia of th... more Patients with peripheral vascular disease (Fontaine stage II) are characterized by ischemia of the lower extremities, atherosclerosis and alteration of blood coagulation and fibrinolysis. A randomized, two-period, crossover design was used to compare the effects of extra-virgin (VO) and refined olive (RO) oils on plasma lipids and lipoprotein composition and LDL oxidation susceptibility in free-living men with peripheral vascular disease. The oils differed in their antioxidant profile (alpha-tocopherol: 300 vs. 200 mg/kg; phenolic compounds 800 vs. 60) and concentration but not in their fatty acid composition. Subjects were randomly assigned to two groups. The first group (n = 12) received VO with which to freely cook all meals for 3 mo, followed by a 3-mo wash-out period; they then received RO for the final 3 mo. The second group (n = 12) consumed the oils in the opposite order. Energy, fat, polyunsaturated fatty acids (PUFA) and alpha-tocopherol intakes were not different when pat...

The protein and mineral composition of different varieties of three different lupin species (Lupi... more The protein and mineral composition of different varieties of three different lupin species (Lupinus albus, Lupinus angustifolius, and Lupinus luteus) and the effect of alpha-galactoside removal by means of a hydroalcoholic extraction process on such composition were studied in relationship to nutrient distribution among the different anatomical parts of the seed (embryo, cotyledon, and seed coat). The extent of processing-derived protein insolubilization was assessed by both chemical and electrophoretic techniques and related to the amount of nitrogen soluble in H(2)O, NaCl, ethanol, NaOH, and sodium dodecyl sulfate/beta-mercaptoethanol (SDS/BME). The alpha-galactoside extraction process caused a significant increase in the amount of total and insoluble nitrogen and decreased the amount of soluble protein nitrogen, without affecting the content of soluble nonprotein nitrogen. alpha-Galactoside extraction was not effective at decreasing the levels of Mn present in lupins, and processing caused an increase in the content of this mineral in all of the species studied with the exception of L. albus var. multolupa. In general, the effect of processing on mineral content varied with the different lupin species, and mineral losses were lower in L. luteus.

Nutrition, 2004

We sought to improve the nutritive utilization of Lens culinaris M. variety vulgaris cultivar Mag... more We sought to improve the nutritive utilization of Lens culinaris M. variety vulgaris cultivar Magda-20 in growing rats by autoclaving the lentil seeds at 120°C for 30 min at an internal pressure of 1 atm and by supplementing the rats with a vitamin-mineral premix. METHODS: Nutritional assessment was based on chemical analysis of total phosphorus, free phosphorus, phytate, magnesium, neutral detergent fiber, and acid detergent fiber contents of the lentils and the digestive and metabolic use of phosphorus and magnesium by the growing rats. RESULTS: The net absorption of phosphorus was greater than the amount of non-phytate phosphorus ingested by the animals fed diets of raw and autoclaved lentil flours. Vitamin-mineral supplementation of raw and autoclaved lentil flour resulted in a significant increase in dietary intake and net absorption of phosphorus and magnesium. For all of the experimental diets tested, there was a direct correlation between phosphorus or magnesium balance and weight gain (r ϭ 0.91 and 0.80, respectively) and between phosphorus or magnesium balance and nitrogen balance (r ϭ 0.91 and 0.87, respectively). CONCLUSIONS: Part of the phytate-phosphorus from raw and autoclaved lentil flour was available during digestion, and hardly any phytate was detected in the feces. Supplementation of raw lentil flour with a vitamin-mineral premix was the most effective treatment for increasing daily food intake, body weight gain, and nutritive use of phosphorus and magnesium. Nutrition 2004;20:794 -799. ©Elsevier Inc. 2004

Journal of Agricultural and Food Chemistry, 1997

Changes in the trypsin inhibitor activity (TIA) of faba beans (Vicia faba L. Major) were investig... more Changes in the trypsin inhibitor activity (TIA) of faba beans (Vicia faba L. Major) were investigated after beans were soaked in distilled water, citric acid, and sodium bicarbonate solutions. The soaking solution was discarded. The effect of cookingsdiscarding both soaking and cooking solutionsswas also studied. Finally, a dry-heating process was examined. Soaking treatment produced a slight decrease of trypsin inhibitor activity. Cooking the presoaked seeds brought about the total or partial removal of TIA, depending on the soaking solution used. TIA was partially removed after dryheating. Trypsin inhibitor activity analyses were completed with 28-day biological trials for raw and dry-heated faba beans. For 21 and 28 days of experimental time food intake, protein intake, and weight gain were similar for rats fed raw and dry-heated faba beans. However, after 28 days protein efficiency ratio (PER) and food transformation index (FTI) improved in rats fed dry-heated faba beans compared with those fed raw faba beans. This was related to lower TIA values found in dry-heated faba beans.

Applied Soil Ecology, 2015

Salinity is the major environmental factor limiting crop production. Alfalfa is a legume with hig... more Salinity is the major environmental factor limiting crop production. Alfalfa is a legume with high nutritional value that establishes a symbiosis relation with Ensifer meliloti. Under saline conditions the alfalfa yield decreases and this symbiosis is affected. The aim of this work is to study the effect of the co-inoculation of alfalfa plants with Halomonas maura (a moderately halophile bacterium) and E. meliloti in saline soils to improve their productivity and growth under greenhouse and field conditions. Alfalfa plants were grown in Leonard jar under greenhouse conditions, using a N-free mineral solution to mimic the conditions of an Orthic Solonchak. Then alfalfa plants were grown in the field in the same soil type. Seeds were inoculated with E. meliloti,H. maura, co-inoculated with E. meliloti and H. Maura, or non-inoculated as a control in both experiments. In greenhouse experiments the co-inoculation of alfalfa plants increased significantly the shoot dry weight (0.64 AE 0.02 vs. 0.79 AE 0.02), the leghaemoglobin content (10.17 AE 0.03 vs. 11.25 AE 0.06) and water potential (À3.12 AE 0.02 vs. À2.79 AE 0.02) compared with the single inoculation with E. meliloti. In the field experiments, biomass of co-inoculated plants clearly outyielded those of plants inoculated with any inoculant. The co-inoculation of H. maura and E. meliloti enhances alfalfa productivity in saline soils, thus contributing to the agricultural exploitation of low productive areas. H. maura and E. meliloti could be considered in formulation of bioinoculants to contribute in the reduction of the overuse of chemical fertilizers and their environmental impacts.

The Science of the total environment, 2010

Copper, manganese and selenium are elements involved in protecting the body against oxidative str... more Copper, manganese and selenium are elements involved in protecting the body against oxidative stress. Determining their plasma level may contribute to assessing the health and nutritional status of populations. The aim of this study was to assess factors influencing copper, manganese and selenium plasma levels in an adult Mediterranean population and to identify groups at risk of deficiency. A cross-sectional survey was carried out in Andalusia, a region in southern Spain. Blood samples were obtained in a random subsample of 340 subjects. Food consumption was assessed by 48-h recall. Height, weight, skinfolds, waist and hip circumferences were measured. Copper, manganese and selenium were measured in plasma. Information about physical exercise, educational level, alcohol and smoking habits was obtained with a structured questionnaire. Plasma copper was found to be higher in women than among men. Hypocupraemia was found in 4.4% of the population, while 9.7% presented hypomanganesemia...

British Journal of Nutrition, 2006

The nutritional composition of the legume Lupinus albus var. multolupa, raw or after a-galactosid... more The nutritional composition of the legume Lupinus albus var. multolupa, raw or after a-galactoside extraction, and its effect on the bioavailability of protein, P, Ca, and Mg by growing rats was evaluated using a balance technique. The protein and dietary fibre content of the lupin flours studied was high, and 89 -94 % of the dietary fibre was present as insoluble dietary fibre. The a-galactoside extraction process did not disrupt the nutritional quality of protein, and the digestive and metabolic utilisation of this nutrient was high and comparable with that obtained from a caseincystine control diet (pair-fed to the average daily food intake of the experimental groups fed the different lupin diets). Bioavailability of P, Ca, and Mg from the lupin diets tested was high, and supplementation of an exogenous microbial phytase (750 phytase units/kg) did not cause any further improvement. Mineral content in the bone tissue (femur and sternum) did not correlate to mineral balance, which, on the other hand, was related to the mineral content of other tissues such as blood, plasma, liver and kidney. Due to its ability to grow under adverse edaphic and climatic conditions and to its good nutritional quality, a-galactoside-free lupin flour supplemented with the required amounts of minerals and vitamins to meet nutrient requirements can be used as an excellent dietary source for the preparation of dietetic products.

British Journal of Nutrition, 2009

The effect of intracerebroventricular or intraperitoneal administration of cannabinoid receptor a... more The effect of intracerebroventricular or intraperitoneal administration of cannabinoid receptor agonist WIN 55,212-2 or inverse agonist AM 251 on food intake and extracellular levels of serotonin and acetic acid 5-hydroxy-indol from presatiated rats was studied. Compared to the vehicleinjected control, the intracerebroventricular administration of WIN 55,212-2 was associated with a significant increase in food intake, whereas the administration of AM 251 caused a significant reduction in this respect. These results were accompanied by considerable reductions or increases in serotonin and acetic acid 5-hydroxy-indol levels compared to the vehicle-injected control and the baseline values for the different experimental groups studied. Intraperitoneal administration of WIN 55,212-2 at doses of 1 and 2 mg/kg promoted hyperphagia up to 6 h after injection, whereas administration of a higher dose (5 mg/kg) significantly inhibited food intake and motor behaviour in partially satiated rats. Administration of any of the AM 251 doses studied (0·5, 1, 2, 5 mg/kg) led to a significant decrease in the amount of food ingested from 2 h after the injection, compared to the vehicle-injected control group, with the most striking effect being observed when the 5 mg/kg dose was injected. Cannabinoid system: CB 1 receptors: Food intake: Nutritional value: WIN 55,212-2: AM 251: Serotonin: Acetic acid 5-hydroxy-indol: c-fos Expression * Corresponding author: Dr María López-Jurado, fax þ34 958 248959, email mlopezj@ugr.es

British Journal of Nutrition, 2011

Consumption of high-protein (HP) diets is postulated to exert a negative influence on bone and re... more Consumption of high-protein (HP) diets is postulated to exert a negative influence on bone and renal health. However, no conclusive evidence has been presented related to this issue or to the potential protective action of resistance training on HP-induced systemic effects. We examined the effects of HP diet consumption on food intake, body-weight gain, body composition, and renal, bone and metabolic parameters of rats performing resistance training. A total of ninety-six adult male Wistar rats were randomly distributed in twelve experimental groups (n 8): normal-protein (10%) or HP (45%) diets, with or without resistance training, killed for experimental periods of 1, 2 or 3 months. Diets were based on a commercial whey protein hydrolysate. Consumption of HP diets and resistance training significantly affected food intake, body weight and body composition, as well as the plasma levels of total cholesterol, HDL-cholesterol and TAG. The buffering action of resistance training on such diet-induced alterations was especially evident in the levels of plasma TAG. Consumption of HP diets led to a considerable increase in kidney weight, urinary volume and acidity, as well as in the urinary excretion of Ca, with a parallel reduction in the urinary excretion of citrate (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0·05). No apparent deleterious effect on bone mineral content was found. In conclusion, consumption of HP diets caused alterations in renal health status and some metabolic parameters, but did not seem to affect bone status. Resistance training had a protective action against alterations of renal health status and some metabolic parameters such as plasma TAG.

Nutrition Research, 1999

The influence of germination for 6 days and dry-heating at 120°C for 15 min on the content of cal... more The influence of germination for 6 days and dry-heating at 120°C for 15 min on the content of calcium, phosphorus, phytic acid and dietary fiber in lentils was studied. Germination lowered total phosphorus (P) and phytic acid content whereas dry-heating did not modify total P but slightly decreased phytic acid. Both treatments increased cellulose (CL) and lignin (LN) content and reduced hemicellulose (HMC) of lentils. The apparent digestibility coefficient @DC) and balance of calcium (Ca) improved in animals fed dry-heated and germinated lentils which was related with the decrease in HMC. Phytic acid intake was significantly reduced by dry-heating and germination, the lowest P intake and P absorption observed in animals fed germinated lentils led to a null P balance. The results obtained in muscle and femur by comparing control (casein) and experimental diets indicated that muscle is more sensitive tissue than bone at lower Ca and P retention. 0 1998 !zk.vi~ scimcc 1°C.

Nutrition, 2005

We assessed the effect of germinating Pisum sativum L. variant Arvense cv. Esla for 3 and 6 d in ... more We assessed the effect of germinating Pisum sativum L. variant Arvense cv. Esla for 3 and 6 d in darkness on the chemical composition and nutritive utilization of protein and carbohydrates. Methods: Nutritional assessment of protein and carbohydrates was based on chemical analysis of raw and germinated pea flours and in vitro and in vivo rat balance methodologies. Results: Germination caused a notable decrease in ␣-galactoside content and significant increases in sucrose, glucose, and fructose. The ratio of available starch to total starch increased as a consequence of processing. The content of vitamin B2 increased significantly, whereas no significant change was observed in vitamin B1 content in germinated peas. Protein digestibility assessed with an in vivo technique (apparent digestibility coefficient) or as the percentage of dialyzable nitrogen increased significantly as a result of germination in contrast to what was observed with the in vitro pH-drop methodology. Daily food intake, nitrogen absorption and balance, percentage of retained versus absorbed nitrogen, protein efficiency ratio, and the index of available carbohydrates were significantly improved by germination for 3 d and significantly decreased by germination for 6 d. Conclusions: Germination of pea seeds for 3 d significantly improves palatability of these seeds and the nutritive utilization of protein and carbohydrates.

Journal of the Science of Food and Agriculture, 2004

There will be a release of Wiley Online Library scheduled for Saturday 27th November 2010. Access... more There will be a release of Wiley Online Library scheduled for Saturday 27th November 2010. Access to the website will be disrupted as follows: New York 0630 EDT to 0830 EDT; London 1130 GMT to 1330 GMT; Singapore 1930 SGT to 2130 SGT

Journal of the Science of Food and Agriculture, 2007

The objective of this study was to obtain functional flours from Pisum sativum L. var. Esla to be... more The objective of this study was to obtain functional flours from Pisum sativum L. var. Esla to be used as an ingredient to be incorporated in the development of new products for human nutrition. With the aim of counteracting the potential anti-nutritional effect of certain non-...

Journal of the American College of Nutrition, 2005

Objective: The aim of the study was to identify factors associated with obesity, and their influe... more Objective: The aim of the study was to identify factors associated with obesity, and their influence on plasma lipid profile in an adult Mediterranean population.

Journal of Physiology and Biochemistry, 2000

This review describes the present state of knowledge about phytic acid (phytate), which is often ... more This review describes the present state of knowledge about phytic acid (phytate), which is often present in legume seeds. The antinutritional effects of phytic acid primarily relate to the strong chelating associated with its six reactive phosphate groups. Its ability to complex with proteins and particularly with minerals has been a subject of investigation from chemical and nutritional viewpoints. The hydrolysis of phytate into inositol and phosphates or phosphoric acid occurs as a result of phytase or nonenzymatic cleavage. Enzymes capable of hydrolysing phytates are widely distributed in micro-organisms, plants and animals. Phytases act in a stepwise manner to catalyse the hydrolysis of phytic acid. To reduce or eliminate the chelating ability of phytate, dephosphorylation of hexa- and penta-phosphate forms is essential since a high degree of phosphorylation is necessary to bind minerals. There are several methods of decreasing the inhibitory effect of phytic acid on mineral absorption (cooking, germination, fermentation, soaking, autolysis). Nevertheless, inositol hexaphosphate is receiving increased attention owing to its role in cancer prevention and/or therapy and its hypocholesterolaemic effect.

The Journal of Nutritional Biochemistry, 2009

This study assessed factors influencing iron and zinc intake and associated biochemical parameter... more This study assessed factors influencing iron and zinc intake and associated biochemical parameters in an adult population from southern Spain to identify patterns of intake and groups at risk for deficiency. A cross-sectional survey was done in Andalusia (southern Spain) to study nutrient intakes in a random sample of 3421 subjects (1747 men, 1674 women, age between 25 and 60 years). Blood samples were obtained for biochemical assays in a random subsample of 354 subjects (170 men, 184 women). Food consumption was assessed by 48-h recall. In blood samples, we measured red blood cells, haemoglobin (Hb), haematocrit, total iron binding capacity (TIBC) and plasma concentration of Fe and Zn. Information about educational level, smoking habit, alcohol consumption and physical exercise was collected with a structured questionnaire. Intakes were below two thirds of the recommended dietary allowances (RDA) in 22.45% of the sample for Fe and in 56.45% for Zn. Iron deficiency [two or more abnormal values for plasma Fe, TIBC, transferrin saturation and mean cell volume (MCV)] was found in 12.7% of the sample, and iron-deficient anaemia (low values for Hb, MCV, mean cell Hb and mean corpuscular Hb concentration) was found in 2.1%. In smokers, plasma levels of Fe were higher, and MCV was lower than in nonsmokers. Plasma Zn concentrations were below the reference value in 17.8% of the persons. Age and body mass index correlated inversely with plasma Zn (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;.01). Gender, age, obesity, smoking, alcohol consumption and physical activity were associated with differences in nutrient intakes. Logistic regression analysis showed that female gender and older age were associated with the risk of low intakes of Fe and Zn.

Journal of Nutritional and Environmental Medicine, 1992

... These commonly found levels of dietary protein intake, although not harmful in Page 6. 112MAR... more ... These commonly found levels of dietary protein intake, although not harmful in Page 6. 112MARU JOSE SANTOS ETAL. themselves, can potentially lead to difficulties because these nutrients are associated with large amounts of saturated fatty acids. ...

Journal of Agricultural and Food Chemistry, 1993

The digestive and metabolic utilizations of vegetable protein concentrates were determined in gro... more The digestive and metabolic utilizations of vegetable protein concentrates were determined in growing rats with the nutritional balance method of Thomas-Mitchell. The test diets contained 12% protein in the form of casein plus 5% DL-methionine (control), commercial potato (PPC) and wheat (WPC) concentrates, and supplemented wheat protein concentrate (sWPC). The chemical composition of the protein sources was also determined to detect possible deficiencies in essential amino acids in PPC and WPC. The aminogram for PPC ...