Gerardo Gascón | Universidad de Guadalajara (original) (raw)

Papers by Gerardo Gascón

Research paper thumbnail of Evaluation of serum levels of cytokines in acute apical abscess: a longitudinal observational study

Research paper thumbnail of TNFSF13B rs9514828 gene polymorphism and soluble B cell activating factor levels: Association with apical periodontitis

International Endodontic Journal

Research paper thumbnail of Successful Orthograde Treatment of Teeth with External Inflammatory Root Resorption and Perforation Using a Tricalcium Silicate-Based Material

Case Reports in Dentistry

One possible consequence of dentoalveolar trauma is the development of external inflammatory root... more One possible consequence of dentoalveolar trauma is the development of external inflammatory root resorption (EIRR), which represents an anatomic and microbiologic challenge for clinicians. This case report describes different strategies implemented for successful endodontic management of teeth with multiple EIRR lesions, highlighting the orthograde root canal filling using a tricalcium silicate-based material (Biodentine, Septodont, Saint-Maur-des-Fossés, France). A 17-year-old female patient presented with severe pain in the anterior maxillary teeth and a history of trauma. Two- and three-dimensional radiographic exams confirmed EIRR in three teeth, with a total of 11 EIRR lesions, three exhibiting communication with the root canal. Therefore, chemo-mechanical preparation complemented by ultrasonic activation of irrigants and some changes of intra-canal dressing with calcium hydroxide were performed to reduce the microbiologic load of the affected teeth as much as possible. Then, ...

Research paper thumbnail of Removal of the Smear Layer

Aims: The aim of the study was to evaluate the ability of a mixture of (castor detergent and papa... more Aims: The aim of the study was to evaluate the ability of a mixture of (castor detergent and papain enzyme) MCP to remove the smear layer by using scanning electron microscope. Materials and Methods: Samples of 45 human extracted was divided in to 3 groups (A, B, and C) n = 15 and prepared endodontically using pro taper system up to size F 3, each group was irrigated with corresponding solution 3 ml in between each file size and 5 minutes as a final irrigant as following: Group A irrigated with distilled water (control negative). Group B irrigated with 2.5% (Sodium hypo chloride) NaOCL and 17% (Ethylene di amine tetra acetic acid) EDTA (control positive). Group C irrigated with 20% castor detergent and 4% papain enzyme as a mixture (MCP). Each sample was irrigated with 15 ml of distilled water and dried with paper points. The samples were sent for SEM photograph. Each sample was evaluated at three levels (apical, middle, and cervical part of the canal) Results: MCP solution showed partial removal of both organic and inorganic parts (dual action) of smear layer from the 3 levels of root canals, but the apical one was significantly less clean than the other two, also it has a more gentle effect of erosion than EDTA with NaOCl. EDTA with NaOCl has the ability to remove the smear layer completely from the canal, but still the apical third was significantly less clean than that of other two. It is impossible to remove the smear layer completely by NaOCl and EDTA without erosion of the inner surface of irrigated canal when EDTA was used as a final irrigant for 5 minutes. Conclusion: solution had the ability to remove the smear layer partially at the three levels of a root canal without dentin erosion. While EDTA had the ability to remove the smear layer completely at the three levels of canal with obvious dentinal erosion. Still the apical area has mechanical and anatomical limitation in root canal irrigation.

Research paper thumbnail of Mandibular Canines with Two Roots and Two Root Canals: Case Report and Literature Review

Case Reports in Dentistry, 2017

Usually, the mandibular canine only has one root and one root canal. However, there has been a no... more Usually, the mandibular canine only has one root and one root canal. However, there has been a noticeable increment in evidence showing variations in its morphology, such as the presence of two roots and two root canals. The aim of this article was to present a case of a mandibular canine with two roots and two root canals and to review the available literature on this anatomic variation. Root canal treatment of tooth #43 with such morphology was performed in a 47-year-old woman. Careful inspection of the preoperative radiograph indicated the presence of more than one canal. The 12-month follow-up showed normal periapical tissues, with no pain or tenderness. Literature review revealed that the overall prevalence of such root canal configuration is 5.7%, with a strong preference for female sex (87.5%). Although mandibular canines with two roots and two root canals are not common, clinicians should always anticipate the presence of possible variations. Therefore, timely diagnosis and ...

Research paper thumbnail of Clinical management of fused and infected mandible canine and incisor

Dental Press Endodontics, 2014

Introduction: Dental fusion is a developmental anomaly in which two teeth buds join each other at... more Introduction: Dental fusion is a developmental anomaly in which two teeth buds join each other at different levels. Objective: To report a case of a lower canine and a lower lateral incisor with separate crowns and root fusion, with root canals connected and apical periodontitis. Methods: One year earlier, the patient had received root canal treatment in the canine; however, there was no remission of symptoms. Endodontic treatment was performed with reinstrumentation, passive ultrasonic irrigation with sodium hypochlorite, smear layer removal and intracanal medication with calcium hydroxide. A week later, the symptoms had disappeared and the canals were filled with gutta-percha and Sealapex by means of the Tagger hybrid technique. Results: After two years and two months, the patient exhibited periapical tissues healing. Conclusion: The detection and proper management of developmental tooth anomaly cases is mandatory for treatment success.

Research paper thumbnail of Removal of an Instrument Fractured by Ultrasound and the Instrument Removal System under Visual Magnification

The Journal of Contemporary Dental Practice, 2015

Aim The case of a lower molar with apical periodontitis, which had previous root canal treatment ... more Aim The case of a lower molar with apical periodontitis, which had previous root canal treatment and a fractured instrument in the distal root beyond the foramen, is presented. Background The simultaneous presence of a foreign body (endodontic instrument or material) in periapical tissues and microorganisms in the root canal, are etiological factors in the formation or maintenance of a periapical lesion, and can lead to failure in endodontic treatment. Case description This instrument was removed through the staging platform technique, by using ultrasound and an Instrument removal system (IRS) microtube under microscope visual amplification. All the canals were re-instrumented, irrigated with sodium hypochlorite and passive ultrasonic irrigation, removal of smear layer and intracanal medication with calcium hydroxide for 8 days, after which they were filled. The symptoms disappeared and clinical and radiograph 2-year follow-up shows healing of periapical tissues. Conclusion The comb...

Research paper thumbnail of Debris remaining in the apical third of root canals after chemomechanical preparation by using sodium hypochlorite and glyde: an in vivo study

Journal of endodontics, 2014

During chemomechanical instrumentation, several liquid or paste substances are used to ease the a... more During chemomechanical instrumentation, several liquid or paste substances are used to ease the action of the files and to eliminate debris and the smear layer. The aim of this study was to evaluate whether the use of a paste containing EDTA during cleaning and shaping of the root canal helps to eliminate debris. Twenty root canals in dog teeth were instrumented by a crown-down technique by using nickel-titanium rotary files. In 10 root canals (group A), sodium hypochlorite was used during instrumentation, followed by a final irrigation with 17% liquid EDTA. In another 10 canals (group B), sodium hypochlorite was again used as the irrigating solution, but Glyde File Prep paste was used with every instrument, and a final irrigation with EDTA was also carried out. Two additional teeth were used as positive and 2 as negative controls. The jaws were prepared for histologic evaluation. In group A where Glyde was not used during cleaning and shaping, little or no debris was found in the a...

Research paper thumbnail of Endodontic management of three-rooted maxillary second premolar in a patient with bilateral occurrence of three roots in maxillary second premolars

Journal of clinical and experimental dentistry, 2012

The possibility of bilateral maxillary second premolars having three separate roots is quite low;... more The possibility of bilateral maxillary second premolars having three separate roots is quite low; however, it must be taken into account in clinical and radiographic evaluations during endodontic treatment. A correct clinical and radiographic diagnosis based on knowledge of root canal anatomy and critical interpretation of radiographs is necessary for a safer and successful endodontic treatment of these teeth. Failure of root canal treatment can occur due to missed roots/canals and often diagnosed when patient experiences continuous post-operative pain and discomfort. This case report describes the root canal treatment of a three-rooted maxillary second premolar in a patient with bilateral occurrence of three roots in maxillary second premolars. Key words:Anatomic variations, maxillary second premolar, radiculous.

Research paper thumbnail of Intra- and Interobserver Agreement during the Assessment of the Different Stages of Root Development Using 4 Radiographic Classifications

Research paper thumbnail of Management of permanent teeth with necrotic pulps and open apices according to the stage of root development

Journal of clinical and experimental dentistry, 2017

This paper analyzed the distribution of treatments for permanent teeth with necrotic pulps and op... more This paper analyzed the distribution of treatments for permanent teeth with necrotic pulps and open apices according to the stage of root development. Dental records from all root canal procedures performed in permanent teeth with necrotic pulps and open apices over a period of 14 years by residents of the Speciality of Endodontics, University of Guadalajara, Mexico, were analized. Records from 206 treatments were mainly divided into the following 3 different stages according to criteria described by Cvek: stage IV (n = 79, 38.3%), stage V (n = 66, 32%) and stage III (n = 53, 25.7%). Few cases involved the initial stages of root development (stages I and II) (n = 8, 3.8%). Such teeth were submitted to four different treatments: MTA apical barrier (n = 69), Ca(OH)2 replacements (n = 34), gutta-percha (n = 67) and a plug of Ca(OH)2/gutta-percha (n = 36). The teeth with intermediate root development (Cvek stage III) showed a predilection for the MTA apical barrier and Ca(OH)2 replaceme...

Research paper thumbnail of Endodontic management of a three-rooted maxillary first premolar

Revista de la Asociación Odontológica Argentina

Aim: To report the endodontic treatment of a three-rooted maxillary first premolar by individuali... more Aim: To report the endodontic treatment of a three-rooted maxillary first premolar by individualized root canal treatment to such cases. Case report: A 25 years old male patient presented to dental office with a diagnosis of asymptomatic irreversible pulpitis in the tooth #14. From the initial clinical and radiographic findings –which were suspecting of an alteration in the number of root canals–, the presence of three root canals was confirmed. This situation leads to perform an endodontic treatment focused in locate all the root canals and to prevent excessive weakness and/or perforations. Conclusion: The early diagnosis of this dental variation allows establishing suitable modifications on the treatment protocol, avoiding excessive weakening or even perforation due to the fragile root anatomy.

Research paper thumbnail of Mandibular canines with two roots and two root canals: case report and literature review

Usually, the mandibular canine only has one root and one root canal. However, there has been a no... more Usually, the mandibular canine only has one root and one root canal. However, there has been a noticeable increment in evidence showing variations in its morphology, such as the presence of two roots and two root canals. The aim of this article was to present a case of a mandibular canine with two roots and two root canals and to review the available literature on this anatomic variation. Root canal treatment of tooth #43 with such morphology was performed in a 47-year-old woman. Careful inspection of the preoperative radiograph indicated the presence of more than one canal. The 12-month follow-up showed normal periapical tissues, with no pain or tenderness. Literature review revealed that the overall prevalence of such root canal configuration is 5.7%, with a strong preference for female sex (87.5%). Although mandibular canines with two roots and two root canals are not common, clinicians should always anticipate the presence of possible variations. Therefore, timely diagnosis and meticulous exploration of such mandibular canines allow for planning of an individualized treatment protocol, tailored to their peculiar morphology, focused on avoiding excessive weakening or even perforation of the roots.

Research paper thumbnail of Clinical management of fused and infected mandible canine and incisor

Introduction: Dental fusion is a developmental anomaly in which two teeth buds join each other at... more Introduction: Dental fusion is a developmental anomaly in which two teeth buds join each other at different levels. Objective: To report a case of a lower canine and a lower lateral incisor with separate crowns and root fusion, with root canals connected and apical peri-odontitis. Methods: One year earlier, the patient had received root canal treatment in the canine; however, there was no remission of symptoms. Endodontic treatment was performed with reinstrumentation, passive ultra-sonic irrigation with sodium hypochlorite, smear layer

Research paper thumbnail of Debris Remaining in the Apical Third of Root Canals after Chemomechanical Preparation by Using Sodium Hypochlorite and Glyde: An In Vivo Study

Introduction: During chemomechanical instrumenta-tion, several liquid or paste substances are use... more Introduction: During chemomechanical instrumenta-tion, several liquid or paste substances are used to ease the action of the files and to eliminate debris and the smear layer. The aim of this study was to evaluate whether the use of a paste containing EDTA during cleaning and shaping of the root canal helps to eliminate debris. Methods: Twenty root canals in dog teeth were instrumented by a crown-down technique by using nickel-titanium rotary files. In 10 root canals (group A), sodium hypochlorite was used during instrumenta-tion, followed by a final irrigation with 17% liquid EDTA. In another 10 canals (group B), sodium hypochlo-rite was again used as the irrigating solution, but Glyde File Prep paste was used with every instrument, and a final irrigation with EDTA was also carried out. Two additional teeth were used as positive and 2 as negative controls. The jaws were prepared for histologic evaluation. Results: In group A where Glyde was not used during cleaning and shaping, little or no debris was found in the apical third of the instrumented root canals; however ; in group B in which Glyde File Prep paste was used during chemomechanical instrumentation, moderate to high accumulation of debris was observed in the apical third. Conclusions: The use of Glyde File Prep paste during rotary mechanical instrumentation favors the accumulation of debris in the apical third of the root canals. Irrigation with NaOCl and a final flush with EDTA by means of a small-gauge needle with simultaneous aspiration led to less accumulation of debris than in the Glyde File Prep group (P < .05). (J Endod 2014;40:1419–1423)

Research paper thumbnail of Evaluation of serum levels of cytokines in acute apical abscess: a longitudinal observational study

Research paper thumbnail of TNFSF13B rs9514828 gene polymorphism and soluble B cell activating factor levels: Association with apical periodontitis

International Endodontic Journal

Research paper thumbnail of Successful Orthograde Treatment of Teeth with External Inflammatory Root Resorption and Perforation Using a Tricalcium Silicate-Based Material

Case Reports in Dentistry

One possible consequence of dentoalveolar trauma is the development of external inflammatory root... more One possible consequence of dentoalveolar trauma is the development of external inflammatory root resorption (EIRR), which represents an anatomic and microbiologic challenge for clinicians. This case report describes different strategies implemented for successful endodontic management of teeth with multiple EIRR lesions, highlighting the orthograde root canal filling using a tricalcium silicate-based material (Biodentine, Septodont, Saint-Maur-des-Fossés, France). A 17-year-old female patient presented with severe pain in the anterior maxillary teeth and a history of trauma. Two- and three-dimensional radiographic exams confirmed EIRR in three teeth, with a total of 11 EIRR lesions, three exhibiting communication with the root canal. Therefore, chemo-mechanical preparation complemented by ultrasonic activation of irrigants and some changes of intra-canal dressing with calcium hydroxide were performed to reduce the microbiologic load of the affected teeth as much as possible. Then, ...

Research paper thumbnail of Removal of the Smear Layer

Aims: The aim of the study was to evaluate the ability of a mixture of (castor detergent and papa... more Aims: The aim of the study was to evaluate the ability of a mixture of (castor detergent and papain enzyme) MCP to remove the smear layer by using scanning electron microscope. Materials and Methods: Samples of 45 human extracted was divided in to 3 groups (A, B, and C) n = 15 and prepared endodontically using pro taper system up to size F 3, each group was irrigated with corresponding solution 3 ml in between each file size and 5 minutes as a final irrigant as following: Group A irrigated with distilled water (control negative). Group B irrigated with 2.5% (Sodium hypo chloride) NaOCL and 17% (Ethylene di amine tetra acetic acid) EDTA (control positive). Group C irrigated with 20% castor detergent and 4% papain enzyme as a mixture (MCP). Each sample was irrigated with 15 ml of distilled water and dried with paper points. The samples were sent for SEM photograph. Each sample was evaluated at three levels (apical, middle, and cervical part of the canal) Results: MCP solution showed partial removal of both organic and inorganic parts (dual action) of smear layer from the 3 levels of root canals, but the apical one was significantly less clean than the other two, also it has a more gentle effect of erosion than EDTA with NaOCl. EDTA with NaOCl has the ability to remove the smear layer completely from the canal, but still the apical third was significantly less clean than that of other two. It is impossible to remove the smear layer completely by NaOCl and EDTA without erosion of the inner surface of irrigated canal when EDTA was used as a final irrigant for 5 minutes. Conclusion: solution had the ability to remove the smear layer partially at the three levels of a root canal without dentin erosion. While EDTA had the ability to remove the smear layer completely at the three levels of canal with obvious dentinal erosion. Still the apical area has mechanical and anatomical limitation in root canal irrigation.

Research paper thumbnail of Mandibular Canines with Two Roots and Two Root Canals: Case Report and Literature Review

Case Reports in Dentistry, 2017

Usually, the mandibular canine only has one root and one root canal. However, there has been a no... more Usually, the mandibular canine only has one root and one root canal. However, there has been a noticeable increment in evidence showing variations in its morphology, such as the presence of two roots and two root canals. The aim of this article was to present a case of a mandibular canine with two roots and two root canals and to review the available literature on this anatomic variation. Root canal treatment of tooth #43 with such morphology was performed in a 47-year-old woman. Careful inspection of the preoperative radiograph indicated the presence of more than one canal. The 12-month follow-up showed normal periapical tissues, with no pain or tenderness. Literature review revealed that the overall prevalence of such root canal configuration is 5.7%, with a strong preference for female sex (87.5%). Although mandibular canines with two roots and two root canals are not common, clinicians should always anticipate the presence of possible variations. Therefore, timely diagnosis and ...

Research paper thumbnail of Clinical management of fused and infected mandible canine and incisor

Dental Press Endodontics, 2014

Introduction: Dental fusion is a developmental anomaly in which two teeth buds join each other at... more Introduction: Dental fusion is a developmental anomaly in which two teeth buds join each other at different levels. Objective: To report a case of a lower canine and a lower lateral incisor with separate crowns and root fusion, with root canals connected and apical periodontitis. Methods: One year earlier, the patient had received root canal treatment in the canine; however, there was no remission of symptoms. Endodontic treatment was performed with reinstrumentation, passive ultrasonic irrigation with sodium hypochlorite, smear layer removal and intracanal medication with calcium hydroxide. A week later, the symptoms had disappeared and the canals were filled with gutta-percha and Sealapex by means of the Tagger hybrid technique. Results: After two years and two months, the patient exhibited periapical tissues healing. Conclusion: The detection and proper management of developmental tooth anomaly cases is mandatory for treatment success.

Research paper thumbnail of Removal of an Instrument Fractured by Ultrasound and the Instrument Removal System under Visual Magnification

The Journal of Contemporary Dental Practice, 2015

Aim The case of a lower molar with apical periodontitis, which had previous root canal treatment ... more Aim The case of a lower molar with apical periodontitis, which had previous root canal treatment and a fractured instrument in the distal root beyond the foramen, is presented. Background The simultaneous presence of a foreign body (endodontic instrument or material) in periapical tissues and microorganisms in the root canal, are etiological factors in the formation or maintenance of a periapical lesion, and can lead to failure in endodontic treatment. Case description This instrument was removed through the staging platform technique, by using ultrasound and an Instrument removal system (IRS) microtube under microscope visual amplification. All the canals were re-instrumented, irrigated with sodium hypochlorite and passive ultrasonic irrigation, removal of smear layer and intracanal medication with calcium hydroxide for 8 days, after which they were filled. The symptoms disappeared and clinical and radiograph 2-year follow-up shows healing of periapical tissues. Conclusion The comb...

Research paper thumbnail of Debris remaining in the apical third of root canals after chemomechanical preparation by using sodium hypochlorite and glyde: an in vivo study

Journal of endodontics, 2014

During chemomechanical instrumentation, several liquid or paste substances are used to ease the a... more During chemomechanical instrumentation, several liquid or paste substances are used to ease the action of the files and to eliminate debris and the smear layer. The aim of this study was to evaluate whether the use of a paste containing EDTA during cleaning and shaping of the root canal helps to eliminate debris. Twenty root canals in dog teeth were instrumented by a crown-down technique by using nickel-titanium rotary files. In 10 root canals (group A), sodium hypochlorite was used during instrumentation, followed by a final irrigation with 17% liquid EDTA. In another 10 canals (group B), sodium hypochlorite was again used as the irrigating solution, but Glyde File Prep paste was used with every instrument, and a final irrigation with EDTA was also carried out. Two additional teeth were used as positive and 2 as negative controls. The jaws were prepared for histologic evaluation. In group A where Glyde was not used during cleaning and shaping, little or no debris was found in the a...

Research paper thumbnail of Endodontic management of three-rooted maxillary second premolar in a patient with bilateral occurrence of three roots in maxillary second premolars

Journal of clinical and experimental dentistry, 2012

The possibility of bilateral maxillary second premolars having three separate roots is quite low;... more The possibility of bilateral maxillary second premolars having three separate roots is quite low; however, it must be taken into account in clinical and radiographic evaluations during endodontic treatment. A correct clinical and radiographic diagnosis based on knowledge of root canal anatomy and critical interpretation of radiographs is necessary for a safer and successful endodontic treatment of these teeth. Failure of root canal treatment can occur due to missed roots/canals and often diagnosed when patient experiences continuous post-operative pain and discomfort. This case report describes the root canal treatment of a three-rooted maxillary second premolar in a patient with bilateral occurrence of three roots in maxillary second premolars. Key words:Anatomic variations, maxillary second premolar, radiculous.

Research paper thumbnail of Intra- and Interobserver Agreement during the Assessment of the Different Stages of Root Development Using 4 Radiographic Classifications

Research paper thumbnail of Management of permanent teeth with necrotic pulps and open apices according to the stage of root development

Journal of clinical and experimental dentistry, 2017

This paper analyzed the distribution of treatments for permanent teeth with necrotic pulps and op... more This paper analyzed the distribution of treatments for permanent teeth with necrotic pulps and open apices according to the stage of root development. Dental records from all root canal procedures performed in permanent teeth with necrotic pulps and open apices over a period of 14 years by residents of the Speciality of Endodontics, University of Guadalajara, Mexico, were analized. Records from 206 treatments were mainly divided into the following 3 different stages according to criteria described by Cvek: stage IV (n = 79, 38.3%), stage V (n = 66, 32%) and stage III (n = 53, 25.7%). Few cases involved the initial stages of root development (stages I and II) (n = 8, 3.8%). Such teeth were submitted to four different treatments: MTA apical barrier (n = 69), Ca(OH)2 replacements (n = 34), gutta-percha (n = 67) and a plug of Ca(OH)2/gutta-percha (n = 36). The teeth with intermediate root development (Cvek stage III) showed a predilection for the MTA apical barrier and Ca(OH)2 replaceme...

Research paper thumbnail of Endodontic management of a three-rooted maxillary first premolar

Revista de la Asociación Odontológica Argentina

Aim: To report the endodontic treatment of a three-rooted maxillary first premolar by individuali... more Aim: To report the endodontic treatment of a three-rooted maxillary first premolar by individualized root canal treatment to such cases. Case report: A 25 years old male patient presented to dental office with a diagnosis of asymptomatic irreversible pulpitis in the tooth #14. From the initial clinical and radiographic findings –which were suspecting of an alteration in the number of root canals–, the presence of three root canals was confirmed. This situation leads to perform an endodontic treatment focused in locate all the root canals and to prevent excessive weakness and/or perforations. Conclusion: The early diagnosis of this dental variation allows establishing suitable modifications on the treatment protocol, avoiding excessive weakening or even perforation due to the fragile root anatomy.

Research paper thumbnail of Mandibular canines with two roots and two root canals: case report and literature review

Usually, the mandibular canine only has one root and one root canal. However, there has been a no... more Usually, the mandibular canine only has one root and one root canal. However, there has been a noticeable increment in evidence showing variations in its morphology, such as the presence of two roots and two root canals. The aim of this article was to present a case of a mandibular canine with two roots and two root canals and to review the available literature on this anatomic variation. Root canal treatment of tooth #43 with such morphology was performed in a 47-year-old woman. Careful inspection of the preoperative radiograph indicated the presence of more than one canal. The 12-month follow-up showed normal periapical tissues, with no pain or tenderness. Literature review revealed that the overall prevalence of such root canal configuration is 5.7%, with a strong preference for female sex (87.5%). Although mandibular canines with two roots and two root canals are not common, clinicians should always anticipate the presence of possible variations. Therefore, timely diagnosis and meticulous exploration of such mandibular canines allow for planning of an individualized treatment protocol, tailored to their peculiar morphology, focused on avoiding excessive weakening or even perforation of the roots.

Research paper thumbnail of Clinical management of fused and infected mandible canine and incisor

Introduction: Dental fusion is a developmental anomaly in which two teeth buds join each other at... more Introduction: Dental fusion is a developmental anomaly in which two teeth buds join each other at different levels. Objective: To report a case of a lower canine and a lower lateral incisor with separate crowns and root fusion, with root canals connected and apical peri-odontitis. Methods: One year earlier, the patient had received root canal treatment in the canine; however, there was no remission of symptoms. Endodontic treatment was performed with reinstrumentation, passive ultra-sonic irrigation with sodium hypochlorite, smear layer

Research paper thumbnail of Debris Remaining in the Apical Third of Root Canals after Chemomechanical Preparation by Using Sodium Hypochlorite and Glyde: An In Vivo Study

Introduction: During chemomechanical instrumenta-tion, several liquid or paste substances are use... more Introduction: During chemomechanical instrumenta-tion, several liquid or paste substances are used to ease the action of the files and to eliminate debris and the smear layer. The aim of this study was to evaluate whether the use of a paste containing EDTA during cleaning and shaping of the root canal helps to eliminate debris. Methods: Twenty root canals in dog teeth were instrumented by a crown-down technique by using nickel-titanium rotary files. In 10 root canals (group A), sodium hypochlorite was used during instrumenta-tion, followed by a final irrigation with 17% liquid EDTA. In another 10 canals (group B), sodium hypochlo-rite was again used as the irrigating solution, but Glyde File Prep paste was used with every instrument, and a final irrigation with EDTA was also carried out. Two additional teeth were used as positive and 2 as negative controls. The jaws were prepared for histologic evaluation. Results: In group A where Glyde was not used during cleaning and shaping, little or no debris was found in the apical third of the instrumented root canals; however ; in group B in which Glyde File Prep paste was used during chemomechanical instrumentation, moderate to high accumulation of debris was observed in the apical third. Conclusions: The use of Glyde File Prep paste during rotary mechanical instrumentation favors the accumulation of debris in the apical third of the root canals. Irrigation with NaOCl and a final flush with EDTA by means of a small-gauge needle with simultaneous aspiration led to less accumulation of debris than in the Glyde File Prep group (P < .05). (J Endod 2014;40:1419–1423)