Ian M Andolina - Profile on Academia.edu (original) (raw)
Papers by Ian M Andolina
the Monkey Thalamus During Visual Behavior Cellular Mechanisms Underlying Activity Patterns in
Immediate Impairment of Acute High Intraocular Pressure on Red-Green and Blue-Yellow Cortical Color Responses in Non-Human Primates
Social Science Research Network, 2022
Research Square (Research Square), Apr 18, 2023
B-vitamins have been evaluated as a useful adjuvant therapy to treat pain. In spite of clinical a... more B-vitamins have been evaluated as a useful adjuvant therapy to treat pain. In spite of clinical and experimental evidence indicating the analgesic effect of B-vitamins, few studies have investigated their effect on aspects of the in ammatory pain response. In the present study, we investigated the analgesic effect of chronic application of B-complex vitamins (Neurobion) using an in ammatory experimental pain model in rats. Nociceptive behavioral responses were evaluated in male Wistar rats after plantar injection of formalin, comparing groups with (TG) and without (CG) Neurobion pretreatment. In addition, neuronal activity in the central pain pathway was evaluated using c-Fos immunohistochemical reactivity and NADPH-d histochemistry. A highly signi cant reduction of painful behaviors such as licking and inching were observed in TG, especially during the secondary phase of the formalin test compared to CG. Results suggest that long-term pre-treatment using Neurobion can have a bene cial effect in reducing the chronic phase of pain. In addition, we observed a downregulation of c-Fos and NADPH-d in dorsal spinal neurons, suggesting that the antinociceptive effect induced by Neurobion could be due to a suppression of nociceptive transmission at the spinal level, particularly in the afferent regions of the dorsal spinal horn, which these neurons utilizing nitric oxide at least as one of their pain neurotransmitters.
eLife, May 17, 2021
The ventral visual pathway is crucially involved in integrating low-level visual features into co... more The ventral visual pathway is crucially involved in integrating low-level visual features into complex representations for objects and scenes. At an intermediate stage of the ventral visual pathway, V4 plays a crucial role in supporting this transformation. Many V4 neurons are selective for shape segments like curves and corners; however, it remains unclear whether these neurons are organized into clustered functional domains, a structural motif common across other visual cortices. Using two-photon calcium imaging in awake macaques, we confirmed and localized cortical domains selective for curves or corners in V4. Single-cell resolution imaging confirmed that curveor corner-selective neurons were spatially clustered into such domains. When tested with hexagonal-segment stimuli, we find that stimulus smoothness is the cardinal difference between curve and corner selectivity in V4. Combining cortical population responses with single-neuron analysis, our results reveal that curves and corners are encoded by neurons clustered into functional domains in V4. This functionally specific population architecture bridges the gap between the early and late cortices of the ventral pathway and may serve to facilitate complex object recognition.
arXiv (Cornell University), Jul 3, 2023
The visual system evolved to process natural scenes, yet most of our understanding of the topolog... more The visual system evolved to process natural scenes, yet most of our understanding of the topology and function of visual cortex derives from studies using artificial stimuli. To gain deeper insights into visual processing of natural scenes, we utilized widefield calcium-imaging of primate V4 in response to many natural images, generating a large dataset of columnar-scale responses. We used this dataset to build a digital twin of V4 via deep learning, generating a detailed topographical map of natural image preferences at each cortical position. The map revealed clustered functional domains for specific classes of natural image features. These ranged from surface-related attributes like color and texture to shape-related features such as edges, curvature, and facial features. We validated the model-predicted domains with additional widefield calcium-imaging and single-cell resolution two-photon imaging. Our study illuminates the detailed topological organization and neural codes in V4 that represent natural scenes.
Equiluminant stimuli help assess the integrity of colour perception and the relationship of colou... more Equiluminant stimuli help assess the integrity of colour perception and the relationship of colour to other visual features. As a result of individual variation, it is necessary to calibrate experimental visual stimuli to suit each individual’s unique equiluminant ratio. Most traditional methods rely on training observers to report their subjective equiluminance point. Such paradigms cannot easily be implemented on pre-verbal or non-verbal observers. One alternative, optokinetic nystagmus (OKN) of the eye in response to motion, is partially compromised by recent observations that changes in attentional fixation affect the OKN. Here, we present a novel Pupil Frequency-Tagging Method (PFTM) for detecting a participant’s unique equiluminance point without verbal instruction and with minimal training. PFTM analyses reflexive pupil oscillations induced by slow (< 2 Hz) temporal alternations between coloured stimuli. Two equiluminant stimuli will induce a similar pupil dilation respons...
Effects of acute high intraocular pressure on red-green and blue-yellow cortical color responses in non-human primates
NeuroImage: Clinical, 2022
The ventral visual pathway is crucially involved in integrating low-level visual features into co... more The ventral visual pathway is crucially involved in integrating low-level visual features into complex representations for objects and scenes. At an intermediate stage of the ventral visual pathway, V4 plays a crucial role in supporting this transformation. Many V4 neurons are selective for shape segments like curves and corners; however, it remains unclear whether these neurons are organized into clustered functional domains, a structural motif common across other visual cortices. Using two-photon calcium imaging in awake macaques, we confirmed and localized cortical domains selective for curves or corners in V4. Single-cell resolution imaging confirmed that curveor corner-selective neurons were spatially clustered into such domains. When tested with hexagonal-segment stimuli, we find that stimulus smoothness is the cardinal difference between curve and corner selectivity in V4. Combining cortical population responses with single-neuron analysis, our results reveal that curves and corners are encoded by neurons clustered into functional domains in V4. This functionally specific population architecture bridges the gap between the early and late cortices of the ventral pathway and may serve to facilitate complex object recognition.
Red Predominance of Gamma band Power in Macaque V1 and V4
Advances in Psychological Science, Dec 31, 2016
Ghost in the Machine: Trans-saccadic Modulation of Figure–ground Segmentation in the Thalamus
Advances in Psychological Science, Dec 31, 2016
The Influence of Two Figures for Figure–Ground Segmentation in the Thalamus
心理科学进展, Aug 13, 2017
Doxorubicin accelerates and enhances AAV-based transgene expression in the cat and rat brain
心理科学进展, Aug 26, 2017
PURPOSE: Recombinant adeno-associated viruses (AAV) are widely used vectors for gene transfer int... more PURPOSE: Recombinant adeno-associated viruses (AAV) are widely used vectors for gene transfer into the central nervous system (CNS).However, Low transduction efficiency andthe delay in transgene expression have hampered it from wide application in such as early embryonic development and the brain of higher mammals for both basic and clinic researches. This study is designed to establish a new means to improve both the efficiency and timing of AAV transduction in transgene expression. METHODS: By co-administration of doxorubicin with AAV8 vector into the cortex of cat, we firstly evaluated the effect of doxorubicin on enhancing vector transfection and transgene expression at different time points after infection. Then, we characterized the dose dependence of doxorubicin on AAV-mediated gene expression by varying the concentration of doxorubicin. The toxicity of doxorubicin was also examined by cell apoptosis assay. RESULTS:Local administration of 10 μg/mL doxorubicin remarkably facilitated AAV8-based gene transfer to neurons, increasing both cell numbers and expression level of GFP. The doxorubicin accelerated GFP expression is observed to appear as earlier as the third day after infection, and increased GFP intensity is persistent through the following multiple weeks in time. Doxorubicin was found to increase the neural expression of GFP at doses between 0.1 ~ 10 μg/mL with little toxicity, but induced neuron loss at a dose higher than 30 μg/mL. CONCLUSIONS:Co-infusion of doxorubicin accelerates AAV-based transgene expression in the cortex of higher mammals. This improved efficiency and timing of AAV transduction might have great potentials in the application for early embryonic development and other neurophysiological research for CNS diseases in higher mammals. </p
OrientationFine and Coarse Discrimination of From Another Angle: Differences in Cortical Coding
Transduction catalysis: Doxorubicin amplifies rAAV-mediated gene expression in the cortex of higher-order vertebrates
iScience, Jun 1, 2021
Summary Rapid and efficient gene transduction via recombinant adeno-associated viruses (rAAVs) is... more Summary Rapid and efficient gene transduction via recombinant adeno-associated viruses (rAAVs) is highly desirable across many basic and clinical research domains. Here, we report that vector co-infusion with doxorubicin, a clinical anti-cancer drug, markedly enhanced rAAV-mediated transgene expression in the cerebral cortex across mammalian species (cat, mouse, and macaque), acting throughout the time period examined and detectable at just three days after transfection. This enhancement showed serotype generality, being common to all rAAV serotypes tested (2, 8, 9, and PHP.eB) and was observed both locally and at remote locations consistent with doxorubicin undergoing retrograde axonal transport. All these effects were observed at doses matching human blood plasma levels in clinical therapy and lacked detectable cytotoxicity as assessed by cell morphology, activity, apoptosis, and behavioral testing. Altogether, this study identifies an effective means to improve the capability and scope of in vivo rAAV applications, amplifying cell transduction at doxorubicin concentrations paralleling medical practice.
Spatial and Temporal Abnormalities of Spontaneous Fixational Saccades and Their Correlates With Positive and Cognitive Symptoms in Schizophrenia
Schizophrenia Bulletin, Apr 17, 2023
ESM for Yin et al July 06 2015
Red Predominance of Gamma band Power in Macaque V1 and V4
Influence of monocular deprivation on corticofugal feedback to the lateral geniculate nucleus
of the Lateral Geniculate Nucleus and Visual Cortex Oscillations and Long-Lasting Correlations in a Model
Visual Stimulation Lateral Geniculate Nucleus of Cat During Brief Dynamics of Spatial Resolution of Single Units in the
the Monkey Thalamus During Visual Behavior Cellular Mechanisms Underlying Activity Patterns in
Immediate Impairment of Acute High Intraocular Pressure on Red-Green and Blue-Yellow Cortical Color Responses in Non-Human Primates
Social Science Research Network, 2022
Research Square (Research Square), Apr 18, 2023
B-vitamins have been evaluated as a useful adjuvant therapy to treat pain. In spite of clinical a... more B-vitamins have been evaluated as a useful adjuvant therapy to treat pain. In spite of clinical and experimental evidence indicating the analgesic effect of B-vitamins, few studies have investigated their effect on aspects of the in ammatory pain response. In the present study, we investigated the analgesic effect of chronic application of B-complex vitamins (Neurobion) using an in ammatory experimental pain model in rats. Nociceptive behavioral responses were evaluated in male Wistar rats after plantar injection of formalin, comparing groups with (TG) and without (CG) Neurobion pretreatment. In addition, neuronal activity in the central pain pathway was evaluated using c-Fos immunohistochemical reactivity and NADPH-d histochemistry. A highly signi cant reduction of painful behaviors such as licking and inching were observed in TG, especially during the secondary phase of the formalin test compared to CG. Results suggest that long-term pre-treatment using Neurobion can have a bene cial effect in reducing the chronic phase of pain. In addition, we observed a downregulation of c-Fos and NADPH-d in dorsal spinal neurons, suggesting that the antinociceptive effect induced by Neurobion could be due to a suppression of nociceptive transmission at the spinal level, particularly in the afferent regions of the dorsal spinal horn, which these neurons utilizing nitric oxide at least as one of their pain neurotransmitters.
eLife, May 17, 2021
The ventral visual pathway is crucially involved in integrating low-level visual features into co... more The ventral visual pathway is crucially involved in integrating low-level visual features into complex representations for objects and scenes. At an intermediate stage of the ventral visual pathway, V4 plays a crucial role in supporting this transformation. Many V4 neurons are selective for shape segments like curves and corners; however, it remains unclear whether these neurons are organized into clustered functional domains, a structural motif common across other visual cortices. Using two-photon calcium imaging in awake macaques, we confirmed and localized cortical domains selective for curves or corners in V4. Single-cell resolution imaging confirmed that curveor corner-selective neurons were spatially clustered into such domains. When tested with hexagonal-segment stimuli, we find that stimulus smoothness is the cardinal difference between curve and corner selectivity in V4. Combining cortical population responses with single-neuron analysis, our results reveal that curves and corners are encoded by neurons clustered into functional domains in V4. This functionally specific population architecture bridges the gap between the early and late cortices of the ventral pathway and may serve to facilitate complex object recognition.
arXiv (Cornell University), Jul 3, 2023
The visual system evolved to process natural scenes, yet most of our understanding of the topolog... more The visual system evolved to process natural scenes, yet most of our understanding of the topology and function of visual cortex derives from studies using artificial stimuli. To gain deeper insights into visual processing of natural scenes, we utilized widefield calcium-imaging of primate V4 in response to many natural images, generating a large dataset of columnar-scale responses. We used this dataset to build a digital twin of V4 via deep learning, generating a detailed topographical map of natural image preferences at each cortical position. The map revealed clustered functional domains for specific classes of natural image features. These ranged from surface-related attributes like color and texture to shape-related features such as edges, curvature, and facial features. We validated the model-predicted domains with additional widefield calcium-imaging and single-cell resolution two-photon imaging. Our study illuminates the detailed topological organization and neural codes in V4 that represent natural scenes.
Equiluminant stimuli help assess the integrity of colour perception and the relationship of colou... more Equiluminant stimuli help assess the integrity of colour perception and the relationship of colour to other visual features. As a result of individual variation, it is necessary to calibrate experimental visual stimuli to suit each individual’s unique equiluminant ratio. Most traditional methods rely on training observers to report their subjective equiluminance point. Such paradigms cannot easily be implemented on pre-verbal or non-verbal observers. One alternative, optokinetic nystagmus (OKN) of the eye in response to motion, is partially compromised by recent observations that changes in attentional fixation affect the OKN. Here, we present a novel Pupil Frequency-Tagging Method (PFTM) for detecting a participant’s unique equiluminance point without verbal instruction and with minimal training. PFTM analyses reflexive pupil oscillations induced by slow (< 2 Hz) temporal alternations between coloured stimuli. Two equiluminant stimuli will induce a similar pupil dilation respons...
Effects of acute high intraocular pressure on red-green and blue-yellow cortical color responses in non-human primates
NeuroImage: Clinical, 2022
The ventral visual pathway is crucially involved in integrating low-level visual features into co... more The ventral visual pathway is crucially involved in integrating low-level visual features into complex representations for objects and scenes. At an intermediate stage of the ventral visual pathway, V4 plays a crucial role in supporting this transformation. Many V4 neurons are selective for shape segments like curves and corners; however, it remains unclear whether these neurons are organized into clustered functional domains, a structural motif common across other visual cortices. Using two-photon calcium imaging in awake macaques, we confirmed and localized cortical domains selective for curves or corners in V4. Single-cell resolution imaging confirmed that curveor corner-selective neurons were spatially clustered into such domains. When tested with hexagonal-segment stimuli, we find that stimulus smoothness is the cardinal difference between curve and corner selectivity in V4. Combining cortical population responses with single-neuron analysis, our results reveal that curves and corners are encoded by neurons clustered into functional domains in V4. This functionally specific population architecture bridges the gap between the early and late cortices of the ventral pathway and may serve to facilitate complex object recognition.
Red Predominance of Gamma band Power in Macaque V1 and V4
Advances in Psychological Science, Dec 31, 2016
Ghost in the Machine: Trans-saccadic Modulation of Figure–ground Segmentation in the Thalamus
Advances in Psychological Science, Dec 31, 2016
The Influence of Two Figures for Figure–Ground Segmentation in the Thalamus
心理科学进展, Aug 13, 2017
Doxorubicin accelerates and enhances AAV-based transgene expression in the cat and rat brain
心理科学进展, Aug 26, 2017
PURPOSE: Recombinant adeno-associated viruses (AAV) are widely used vectors for gene transfer int... more PURPOSE: Recombinant adeno-associated viruses (AAV) are widely used vectors for gene transfer into the central nervous system (CNS).However, Low transduction efficiency andthe delay in transgene expression have hampered it from wide application in such as early embryonic development and the brain of higher mammals for both basic and clinic researches. This study is designed to establish a new means to improve both the efficiency and timing of AAV transduction in transgene expression. METHODS: By co-administration of doxorubicin with AAV8 vector into the cortex of cat, we firstly evaluated the effect of doxorubicin on enhancing vector transfection and transgene expression at different time points after infection. Then, we characterized the dose dependence of doxorubicin on AAV-mediated gene expression by varying the concentration of doxorubicin. The toxicity of doxorubicin was also examined by cell apoptosis assay. RESULTS:Local administration of 10 μg/mL doxorubicin remarkably facilitated AAV8-based gene transfer to neurons, increasing both cell numbers and expression level of GFP. The doxorubicin accelerated GFP expression is observed to appear as earlier as the third day after infection, and increased GFP intensity is persistent through the following multiple weeks in time. Doxorubicin was found to increase the neural expression of GFP at doses between 0.1 ~ 10 μg/mL with little toxicity, but induced neuron loss at a dose higher than 30 μg/mL. CONCLUSIONS:Co-infusion of doxorubicin accelerates AAV-based transgene expression in the cortex of higher mammals. This improved efficiency and timing of AAV transduction might have great potentials in the application for early embryonic development and other neurophysiological research for CNS diseases in higher mammals. </p
OrientationFine and Coarse Discrimination of From Another Angle: Differences in Cortical Coding
Transduction catalysis: Doxorubicin amplifies rAAV-mediated gene expression in the cortex of higher-order vertebrates
iScience, Jun 1, 2021
Summary Rapid and efficient gene transduction via recombinant adeno-associated viruses (rAAVs) is... more Summary Rapid and efficient gene transduction via recombinant adeno-associated viruses (rAAVs) is highly desirable across many basic and clinical research domains. Here, we report that vector co-infusion with doxorubicin, a clinical anti-cancer drug, markedly enhanced rAAV-mediated transgene expression in the cerebral cortex across mammalian species (cat, mouse, and macaque), acting throughout the time period examined and detectable at just three days after transfection. This enhancement showed serotype generality, being common to all rAAV serotypes tested (2, 8, 9, and PHP.eB) and was observed both locally and at remote locations consistent with doxorubicin undergoing retrograde axonal transport. All these effects were observed at doses matching human blood plasma levels in clinical therapy and lacked detectable cytotoxicity as assessed by cell morphology, activity, apoptosis, and behavioral testing. Altogether, this study identifies an effective means to improve the capability and scope of in vivo rAAV applications, amplifying cell transduction at doxorubicin concentrations paralleling medical practice.
Spatial and Temporal Abnormalities of Spontaneous Fixational Saccades and Their Correlates With Positive and Cognitive Symptoms in Schizophrenia
Schizophrenia Bulletin, Apr 17, 2023
ESM for Yin et al July 06 2015
Red Predominance of Gamma band Power in Macaque V1 and V4
Influence of monocular deprivation on corticofugal feedback to the lateral geniculate nucleus
of the Lateral Geniculate Nucleus and Visual Cortex Oscillations and Long-Lasting Correlations in a Model
Visual Stimulation Lateral Geniculate Nucleus of Cat During Brief Dynamics of Spatial Resolution of Single Units in the