Ian M Andolina | Chinese Academy of Sciences (original) (raw)

Papers by Ian M Andolina

Social Science Research Network, 2022

Research Square (Research Square), Apr 18, 2023

arXiv (Cornell University), Jul 3, 2023

Equiluminant stimuli help assess the integrity of colour perception and the relationship of colou... more Equiluminant stimuli help assess the integrity of colour perception and the relationship of colour to other visual features. As a result of individual variation, it is necessary to calibrate experimental visual stimuli to suit each individual’s unique equiluminant ratio. Most traditional methods rely on training observers to report their subjective equiluminance point. Such paradigms cannot easily be implemented on pre-verbal or non-verbal observers. One alternative, optokinetic nystagmus (OKN) of the eye in response to motion, is partially compromised by recent observations that changes in attentional fixation affect the OKN. Here, we present a novel Pupil Frequency-Tagging Method (PFTM) for detecting a participant’s unique equiluminance point without verbal instruction and with minimal training. PFTM analyses reflexive pupil oscillations induced by slow (< 2 Hz) temporal alternations between coloured stimuli. Two equiluminant stimuli will induce a similar pupil dilation respons...

NeuroImage: Clinical, 2022

The ventral visual pathway is crucially involved in integrating low-level visual features into co... more The ventral visual pathway is crucially involved in integrating low-level visual features into complex representations for objects and scenes. At an intermediate stage of the ventral visual pathway, V4 plays a crucial role in supporting this transformation. Many V4 neurons are selective for shape segments like curves and corners; however, it remains unclear whether these neurons are organized into clustered functional domains, a structural motif common across other visual cortices. Using two-photon calcium imaging in awake macaques, we confirmed and localized cortical domains selective for curves or corners in V4. Single-cell resolution imaging confirmed that curveor corner-selective neurons were spatially clustered into such domains. When tested with hexagonal-segment stimuli, we find that stimulus smoothness is the cardinal difference between curve and corner selectivity in V4. Combining cortical population responses with single-neuron analysis, our results reveal that curves and corners are encoded by neurons clustered into functional domains in V4. This functionally specific population architecture bridges the gap between the early and late cortices of the ventral pathway and may serve to facilitate complex object recognition.

Advances in Psychological Science, Dec 31, 2016

心理科学进展, Aug 26, 2017

PURPOSE: Recombinant adeno-associated viruses (AAV) are widely used vectors for gene transfer int... more PURPOSE: Recombinant adeno-associated viruses (AAV) are widely used vectors for gene transfer into the central nervous system (CNS).However, Low transduction efficiency andthe delay in transgene expression have hampered it from wide application in such as early embryonic development and the brain of higher mammals for both basic and clinic researches. This study is designed to establish a new means to improve both the efficiency and timing of AAV transduction in transgene expression.   METHODS: By co-administration of doxorubicin with AAV8 vector into the cortex of cat, we firstly evaluated the effect of doxorubicin on enhancing vector transfection and transgene expression at different time points after infection. Then, we characterized the dose dependence of doxorubicin on AAV-mediated gene expression by varying the concentration of doxorubicin. The toxicity of doxorubicin was also examined by cell apoptosis assay.    RESULTS:Local administration of 10 μg/mL doxorubicin remarkably facilitated AAV8-based gene transfer to neurons, increasing both cell numbers and expression level of GFP. The doxorubicin accelerated GFP expression is observed to appear as earlier as the third day after infection, and increased GFP intensity is persistent through the following multiple weeks in time. Doxorubicin was found to increase the neural expression of GFP at doses between 0.1 ~ 10 μg/mL with little toxicity, but induced neuron loss at a dose higher than 30 μg/mL. CONCLUSIONS:Co-infusion of doxorubicin accelerates AAV-based transgene expression in the cortex of higher mammals. This improved efficiency and timing of AAV transduction might have great potentials in the application for early embryonic development and other neurophysiological research for CNS diseases in higher mammals.       </p

iScience, Jun 1, 2021

Summary Rapid and efficient gene transduction via recombinant adeno-associated viruses (rAAVs) is... more Summary Rapid and efficient gene transduction via recombinant adeno-associated viruses (rAAVs) is highly desirable across many basic and clinical research domains. Here, we report that vector co-infusion with doxorubicin, a clinical anti-cancer drug, markedly enhanced rAAV-mediated transgene expression in the cerebral cortex across mammalian species (cat, mouse, and macaque), acting throughout the time period examined and detectable at just three days after transfection. This enhancement showed serotype generality, being common to all rAAV serotypes tested (2, 8, 9, and PHP.eB) and was observed both locally and at remote locations consistent with doxorubicin undergoing retrograde axonal transport. All these effects were observed at doses matching human blood plasma levels in clinical therapy and lacked detectable cytotoxicity as assessed by cell morphology, activity, apoptosis, and behavioral testing. Altogether, this study identifies an effective means to improve the capability and scope of in vivo rAAV applications, amplifying cell transduction at doxorubicin concentrations paralleling medical practice.

Schizophrenia Bulletin, Apr 17, 2023