Le Kang - Chinese Academy of Sciences (original) (raw)
Papers by Le Kang
MicroRNA-dependent development revealed by RNA interference-mediated gene silencing of LmDicer1 in the migratory locust
MicroRNAs (miRNAs) are small noncoding RNAs, which participate in many biological processes. The ... more MicroRNAs (miRNAs) are small noncoding RNAs, which participate in many biological processes. The small RNA transcriptome in the migratory locust has been characterized and 50 conserved miRNA families and 185 potential locust-specific miRNA family candidates have been identified using high-throughput sequencing. However, it is unclear whether miRNAs influence a wide variety of locusts' biological processes, such as growth or development. In insects, Dicer1 ribonuclease transforms miRNA precursors into mature miRNAs. Thus, using systemic RNA interference (RNAi) to silence the expression of Dicer1 in the migratory locust, Locusta migratoria, we reduced miRNA contents in the locust and disrupted two types of molt (nymph-nymph, and nymph-adult). The RNAi of LmDicer1 also resulted in a high mortality in L. migratora. Our study revealed that LmDicer1 was essential for miRNA regulation and development of L. migratoria. These results further support our notion that LmDicer1 could serve as an excellent target for developing novel strategies for controlling this important insect pest.
Chill injury in the eggs of the migratory locust, Locusta migratoria (Orthoptera: Acrididae): the time-temperature relationship with high-temperature interruption
Insect Science, 2005
... The importance of measuring survival in insects at temperature fluctuation below and above ze... more ... The importance of measuring survival in insects at temperature fluctuation below and above zero, as happens in mild temperate winters, has been stressed by Bale (1991) and others (Wang &Kang, 2003; Chen & Kang, 2004). ... housefly Musca domestica (Rojas & Leopold, 1996 ...
The role of plant odours in the leafminer Liriomyza sativae (Diptera: Agromyzidae) and its parasitoid Diglyphus isaea (Hymenoptera: Eulophidae): Orientation towards the host habitat
European Journal of Entomology, 2002
... YUN XIAN ZHAO and LE KANGl ... Larval and adult conditioning of D. isaea affected slightly th... more ... YUN XIAN ZHAO and LE KANGl ... Larval and adult conditioning of D. isaea affected slightly the females' selection of plant type on which the host larvae were located (Coaker & Cheah, 1993). In this study, D. isaea pupae were removed from the plants before their emergence. ...
Revision of the Genus Pseudopsyra Hebrad (Orthoptera: Tettigoniidae: Phaneropterinae), with the Description of Two New Species from China
Http Dx Doi Org 10 3157 0013 872x117 57 Rotgph 2 0 Co 2, 2006
Cryo Letters, Aug 31, 2003
A deep learning approach to document image quality assessment
2014 Ieee International Conference on Image Processing, Oct 1, 2014
FEBS open bio, 2012
Real-time quantitative polymerase chain reaction (qPCR) is a routine and robust approach for meas... more Real-time quantitative polymerase chain reaction (qPCR) is a routine and robust approach for measuring gene expression. The stability of reference genes in qPCR is crucial for the accurate quantification of gene expression. To provide reliable reference genes for studying the transcriptional responses of locust muscles to hypobaric hypoxia, we first examined the gene expression stability of the frequently used housekeeping genes 18S, GAPDH, and β-actin. However, the expression of these three housekeeping genes was influenced by hypobaric hypoxia. Consequently, we identified five novel candidate reference genes from the locust microarray data. The gene expression stability of the five candidates, together with the three classical housekeeping genes, were evaluated using two distinct algorithms implemented in geNorm and NormFinder. GeNorm identified Ach (acetyl-CoA hydrolase) and Pgp (phosphoglycolate phosphatase-like) as the most stable genes and NormFinder further distinguished Ach ...
Review of the genus Holochlora Stål (Orthoptera, Tettigoniidae, Phaneropterinae) from China
Deutsche Entomologische Zeitschrift, 2008
Electrophysiological and Behavioral Responses of a Parasitic Wasp to Plant Volatiles Induced by Two Leaf Miner Species
Chemical Senses, Jun 1, 2006
Science in China Series C Life Sciences, May 1, 2004
Legends and Supplementary Tables S1-S5.
Antennal sensilla of grasshoppers (Orthoptera: Acrididae) in rel ation to food preferences and habits
J Biosciences, 2003
The external structure, i.e. number and distribution of sensillae on male and female antennae of ... more The external structure, i.e. number and distribution of sensillae on male and female antennae of 12 species of grasshoppers belonging to Pamphaginae, Catantopinae, Oedipodinae and Gomphocerinae in the grasslands of Inner Mongolia was investigated using scanning electron microscopy. Five major types of antennal sensillae were detected--trichoid, long basiconic, short basiconic, slender and short basiconic, and coeloconic sensillae. Total number of antennal sensillae varied among different sexes, subfamilies, feeding groups, life forms and eco-forms. Males showed significantly more sensillae than females, due to presence of more short basiconic and coeloconic sensillae. Species under Catantopinae showed more long basiconic sensillae than the others. The Oedipodinae had the highest number of slender and short basiconic sensillae and coeloconic sensillae, followed by Catantopinae and Gomphocerinae; while Pamphaginae had the fewest. The total number of sensillae showed the same trend for these two types amongst the subfamilies as well, species which prefer habits on the ground possessed fewer antennal sensillae than species which prefer to stay on plants. The maximal number of antennal sensillae were observed in hygrophytous species, Chorthippus albomarginatus, in the 12 grasshopper species investigated, although the data is not statistically significant. The general trend which emerged was that species feeding on grass possessed more antennal sensillae, particularly coeloconic sensillae, compared to other feeding group species.
Chemical Senses, 2006
In the present study, Y-tube olfactometric assays demonstrated that headspace volatile extracts c... more In the present study, Y-tube olfactometric assays demonstrated that headspace volatile extracts collected from leaf miner- damaged, or artificially damaged, bean plants were more attractive to naive females of the parasitoid insect Opius dissitus than those collected from healthy plants. Headspace extracts from both Liriomyza huidobrensis and Liriomyza sativae second-instar larvae-damaged beans were analyzed by coupled gas chromatography-electroantennographic detection (GC-EAD).
Commitment and dedication of a Chinese plant physiologist
Protein Cell, Oct 1, 2010
Genome-wide identification and developmental expression profiling of long noncoding RNAs during Drosophila metamorphosis
Scientific reports, 2016
An increasing number of long noncoding RNAs (lncRNAs) have been discovered with the recent advanc... more An increasing number of long noncoding RNAs (lncRNAs) have been discovered with the recent advances in RNA-sequencing technologies. lncRNAs play key roles across diverse biological processes, and are involved in developmental regulation. However, knowledge about how the genome-wide expression of lncRNAs is developmentally regulated is still limited. We here performed a whole-genome identification of lncRNAs followed by a global expression profiling of these lncRNAs during development in Drosophila melanogaster. We combined bioinformatic prediction of lncRNAs with stringent filtering of protein-coding transcripts and experimental validation to define a high-confidence set of Drosophila lncRNAs. We identified 1,077 lncRNAs in the given transcriptomes that contain 43,967 transcripts; among these, 646 lncRNAs are novel. In vivo expression profiling of these lncRNAs in 27 developmental processes revealed that the expression of lncRNAs is highly temporally restricted relative to that of p...
Plant Cell Environ, 2010
Interactions between the root-knot nematode Meloidogyne incognita and three isogenic tomato (Lyco... more Interactions between the root-knot nematode Meloidogyne incognita and three isogenic tomato (Lycopersicon esculentum) genotypes were examined when plants were grown under ambient (370 ppm) and elevated (750 ppm) CO2. We tested the hypothesis that, defence-recessive genotypes tend to allocate 'extra' carbon (relative to nitrogen) to growth under elevated CO2, whereas defence-dominated genotypes allocate extra carbon to defence, and thereby increases the defence against nematodes. For all three genotypes, elevated CO2 increased height, biomass, and root and leaf total non-structural carbohydrates (TNC):N ratio, and decreased amino acids and proteins in leaves. The activity of anti-oxidant enzymes (superoxide dismutase and catalase) was enhanced by nematode infection in defencerecessive genotypes. Furthermore, elevated CO2 and nematode infection did not qualitatively change the volatile organic compounds (VOC) emitted from plants. Elevated CO2 increased the VOC emission rate only for defencedominated genotypes that were not infected with nematodes. Elevated CO2 increased the number of nematodeinduced galls on defence-dominated genotypes but not on wild-types or defence-recessive genotypes roots. Our results suggest that CO2 enrichment may not only increase plant C : N ratio but can disrupt the allocation of plant resources between growth and defence in some genetically modified plants and thereby reduce their resistance to nematodes.
Performances of survival, feeding behavior, and gene expression in aphids reveal their different fitness to host alteration
Scientific reports, 2016
Insect populations feeding on different plant species are under selection pressure to adapt to th... more Insect populations feeding on different plant species are under selection pressure to adapt to these differences. A study integrating elements of the ecology, behavior, and gene expression of aphids on different host plants has not yet been well-explored. The present study explores the relationship between host fitness and survival, feeding behavior, and salivary gland gene expression of a pea (Pisum sativum) host race of Acyrthosiphon pisum feeding on a common host Vicia faba and on three genetically-related hosts (Vicia villosa, Medicago truncatula, and Medicago sativa). Life table data indicated that aphids on non-favored hosts exhibited small size, low reproduction rate, slow population increase and individual development, and long lifespan. Electrical penetration graph results showed that the aphids spent significantly less time in passive ingestion of phloem sap on all non-preferred host plants before acclimation. After a period of acclimation on M. truncatula and V. villosa, ...
Supercooling capacity and cold hardiness of the eggs of the grasshopper Chorthippus fallax (Orthoptera: Acrididae)
European Journal of Entomology, 2004
MicroRNA-dependent development revealed by RNA interference-mediated gene silencing of LmDicer1 in the migratory locust
MicroRNAs (miRNAs) are small noncoding RNAs, which participate in many biological processes. The ... more MicroRNAs (miRNAs) are small noncoding RNAs, which participate in many biological processes. The small RNA transcriptome in the migratory locust has been characterized and 50 conserved miRNA families and 185 potential locust-specific miRNA family candidates have been identified using high-throughput sequencing. However, it is unclear whether miRNAs influence a wide variety of locusts' biological processes, such as growth or development. In insects, Dicer1 ribonuclease transforms miRNA precursors into mature miRNAs. Thus, using systemic RNA interference (RNAi) to silence the expression of Dicer1 in the migratory locust, Locusta migratoria, we reduced miRNA contents in the locust and disrupted two types of molt (nymph-nymph, and nymph-adult). The RNAi of LmDicer1 also resulted in a high mortality in L. migratora. Our study revealed that LmDicer1 was essential for miRNA regulation and development of L. migratoria. These results further support our notion that LmDicer1 could serve as an excellent target for developing novel strategies for controlling this important insect pest.
Chill injury in the eggs of the migratory locust, Locusta migratoria (Orthoptera: Acrididae): the time-temperature relationship with high-temperature interruption
Insect Science, 2005
... The importance of measuring survival in insects at temperature fluctuation below and above ze... more ... The importance of measuring survival in insects at temperature fluctuation below and above zero, as happens in mild temperate winters, has been stressed by Bale (1991) and others (Wang &Kang, 2003; Chen & Kang, 2004). ... housefly Musca domestica (Rojas & Leopold, 1996 ...
The role of plant odours in the leafminer Liriomyza sativae (Diptera: Agromyzidae) and its parasitoid Diglyphus isaea (Hymenoptera: Eulophidae): Orientation towards the host habitat
European Journal of Entomology, 2002
... YUN XIAN ZHAO and LE KANGl ... Larval and adult conditioning of D. isaea affected slightly th... more ... YUN XIAN ZHAO and LE KANGl ... Larval and adult conditioning of D. isaea affected slightly the females' selection of plant type on which the host larvae were located (Coaker & Cheah, 1993). In this study, D. isaea pupae were removed from the plants before their emergence. ...
Revision of the Genus Pseudopsyra Hebrad (Orthoptera: Tettigoniidae: Phaneropterinae), with the Description of Two New Species from China
Http Dx Doi Org 10 3157 0013 872x117 57 Rotgph 2 0 Co 2, 2006
Cryo Letters, Aug 31, 2003
A deep learning approach to document image quality assessment
2014 Ieee International Conference on Image Processing, Oct 1, 2014
FEBS open bio, 2012
Real-time quantitative polymerase chain reaction (qPCR) is a routine and robust approach for meas... more Real-time quantitative polymerase chain reaction (qPCR) is a routine and robust approach for measuring gene expression. The stability of reference genes in qPCR is crucial for the accurate quantification of gene expression. To provide reliable reference genes for studying the transcriptional responses of locust muscles to hypobaric hypoxia, we first examined the gene expression stability of the frequently used housekeeping genes 18S, GAPDH, and β-actin. However, the expression of these three housekeeping genes was influenced by hypobaric hypoxia. Consequently, we identified five novel candidate reference genes from the locust microarray data. The gene expression stability of the five candidates, together with the three classical housekeeping genes, were evaluated using two distinct algorithms implemented in geNorm and NormFinder. GeNorm identified Ach (acetyl-CoA hydrolase) and Pgp (phosphoglycolate phosphatase-like) as the most stable genes and NormFinder further distinguished Ach ...
Review of the genus Holochlora Stål (Orthoptera, Tettigoniidae, Phaneropterinae) from China
Deutsche Entomologische Zeitschrift, 2008
Electrophysiological and Behavioral Responses of a Parasitic Wasp to Plant Volatiles Induced by Two Leaf Miner Species
Chemical Senses, Jun 1, 2006
Science in China Series C Life Sciences, May 1, 2004
Legends and Supplementary Tables S1-S5.
Antennal sensilla of grasshoppers (Orthoptera: Acrididae) in rel ation to food preferences and habits
J Biosciences, 2003
The external structure, i.e. number and distribution of sensillae on male and female antennae of ... more The external structure, i.e. number and distribution of sensillae on male and female antennae of 12 species of grasshoppers belonging to Pamphaginae, Catantopinae, Oedipodinae and Gomphocerinae in the grasslands of Inner Mongolia was investigated using scanning electron microscopy. Five major types of antennal sensillae were detected--trichoid, long basiconic, short basiconic, slender and short basiconic, and coeloconic sensillae. Total number of antennal sensillae varied among different sexes, subfamilies, feeding groups, life forms and eco-forms. Males showed significantly more sensillae than females, due to presence of more short basiconic and coeloconic sensillae. Species under Catantopinae showed more long basiconic sensillae than the others. The Oedipodinae had the highest number of slender and short basiconic sensillae and coeloconic sensillae, followed by Catantopinae and Gomphocerinae; while Pamphaginae had the fewest. The total number of sensillae showed the same trend for these two types amongst the subfamilies as well, species which prefer habits on the ground possessed fewer antennal sensillae than species which prefer to stay on plants. The maximal number of antennal sensillae were observed in hygrophytous species, Chorthippus albomarginatus, in the 12 grasshopper species investigated, although the data is not statistically significant. The general trend which emerged was that species feeding on grass possessed more antennal sensillae, particularly coeloconic sensillae, compared to other feeding group species.
Chemical Senses, 2006
In the present study, Y-tube olfactometric assays demonstrated that headspace volatile extracts c... more In the present study, Y-tube olfactometric assays demonstrated that headspace volatile extracts collected from leaf miner- damaged, or artificially damaged, bean plants were more attractive to naive females of the parasitoid insect Opius dissitus than those collected from healthy plants. Headspace extracts from both Liriomyza huidobrensis and Liriomyza sativae second-instar larvae-damaged beans were analyzed by coupled gas chromatography-electroantennographic detection (GC-EAD).
Commitment and dedication of a Chinese plant physiologist
Protein Cell, Oct 1, 2010
Genome-wide identification and developmental expression profiling of long noncoding RNAs during Drosophila metamorphosis
Scientific reports, 2016
An increasing number of long noncoding RNAs (lncRNAs) have been discovered with the recent advanc... more An increasing number of long noncoding RNAs (lncRNAs) have been discovered with the recent advances in RNA-sequencing technologies. lncRNAs play key roles across diverse biological processes, and are involved in developmental regulation. However, knowledge about how the genome-wide expression of lncRNAs is developmentally regulated is still limited. We here performed a whole-genome identification of lncRNAs followed by a global expression profiling of these lncRNAs during development in Drosophila melanogaster. We combined bioinformatic prediction of lncRNAs with stringent filtering of protein-coding transcripts and experimental validation to define a high-confidence set of Drosophila lncRNAs. We identified 1,077 lncRNAs in the given transcriptomes that contain 43,967 transcripts; among these, 646 lncRNAs are novel. In vivo expression profiling of these lncRNAs in 27 developmental processes revealed that the expression of lncRNAs is highly temporally restricted relative to that of p...
Plant Cell Environ, 2010
Interactions between the root-knot nematode Meloidogyne incognita and three isogenic tomato (Lyco... more Interactions between the root-knot nematode Meloidogyne incognita and three isogenic tomato (Lycopersicon esculentum) genotypes were examined when plants were grown under ambient (370 ppm) and elevated (750 ppm) CO2. We tested the hypothesis that, defence-recessive genotypes tend to allocate 'extra' carbon (relative to nitrogen) to growth under elevated CO2, whereas defence-dominated genotypes allocate extra carbon to defence, and thereby increases the defence against nematodes. For all three genotypes, elevated CO2 increased height, biomass, and root and leaf total non-structural carbohydrates (TNC):N ratio, and decreased amino acids and proteins in leaves. The activity of anti-oxidant enzymes (superoxide dismutase and catalase) was enhanced by nematode infection in defencerecessive genotypes. Furthermore, elevated CO2 and nematode infection did not qualitatively change the volatile organic compounds (VOC) emitted from plants. Elevated CO2 increased the VOC emission rate only for defencedominated genotypes that were not infected with nematodes. Elevated CO2 increased the number of nematodeinduced galls on defence-dominated genotypes but not on wild-types or defence-recessive genotypes roots. Our results suggest that CO2 enrichment may not only increase plant C : N ratio but can disrupt the allocation of plant resources between growth and defence in some genetically modified plants and thereby reduce their resistance to nematodes.
Performances of survival, feeding behavior, and gene expression in aphids reveal their different fitness to host alteration
Scientific reports, 2016
Insect populations feeding on different plant species are under selection pressure to adapt to th... more Insect populations feeding on different plant species are under selection pressure to adapt to these differences. A study integrating elements of the ecology, behavior, and gene expression of aphids on different host plants has not yet been well-explored. The present study explores the relationship between host fitness and survival, feeding behavior, and salivary gland gene expression of a pea (Pisum sativum) host race of Acyrthosiphon pisum feeding on a common host Vicia faba and on three genetically-related hosts (Vicia villosa, Medicago truncatula, and Medicago sativa). Life table data indicated that aphids on non-favored hosts exhibited small size, low reproduction rate, slow population increase and individual development, and long lifespan. Electrical penetration graph results showed that the aphids spent significantly less time in passive ingestion of phloem sap on all non-preferred host plants before acclimation. After a period of acclimation on M. truncatula and V. villosa, ...
Supercooling capacity and cold hardiness of the eggs of the grasshopper Chorthippus fallax (Orthoptera: Acrididae)
European Journal of Entomology, 2004