Dr. Saber Abd-Allah | Chinese Academy of Sciences (original) (raw)
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Papers by Dr. Saber Abd-Allah
British Journal of Pharmacology, Jan 1, 2012
Abstract: The present study was conducted to investigate the effects of addition of different con... more Abstract: The present study was conducted to investigate the effects of addition of different concentrations (0.1, 0.2, 0.2, 0.4 and 0.5%, respectively) of Royal Jelly on the viability and maturation of the Egyptian sheep oocytes during in vitro culture of oocytes in serum ...
Buffalo Bulletin (March 2011), Jan 1, 2011
... Saber Mohammed Abd-Allah Theriogenology Department, Faculty of Veterinary Medicine, Beni-Suef... more ... Saber Mohammed Abd-Allah Theriogenology Department, Faculty of Veterinary Medicine, Beni-Suef University, 62511 Beni-Suef,Egypt Original Article Page 2. Buffalo Bulletin (March 2011) Vol.30 No.1 46 occur through simple diffusion. ... al., 1989; McWilliams et al., 1991). ...
Journal of Buffalo Science, Jan 1, 2012
The aim of the present study was to assess the effect of addition of Royal jelly in presence of h... more The aim of the present study was to assess the effect of addition of Royal jelly in presence of heparin on buffalo (Bubalus Bubalis) sperm motility, acrosome reaction and in vitro fertilization (IVF) of buffalo oocytes. Frozen buffalo spermatozoa from five bulls were thawed and motile fraction was obtained by swim up technique. The spermatozoa were washed, treated with100 µg/ml heparin, and then exposed to 0.4% Royal Jelly (RJ) for 3 h. Sperm motility, acrosomal integrity and fertilization rate of matured oocytes were assessed at 1, 2 and 3 h. The percentages of sperm motility, intact acrosome and fertilization rate of matured oocytes were higher (P<0.05) in 0.4% RJ compared to that in the control. After 2 h of incubation the percentage of motility, intact acrosome of spermatozoa and fertilization rate of matured oocytes, respectively, were 93.6 %, 77.6% and 72.6% in 0.4% RJ. These results suggest that treating buffalo sperm with 0.4% RJ in combination with heparin is effective not only to induce sperm acrosome reaction but also is effective for in vitro fertilizing capacity of the cryopreserved buffalo spermatozoa.
Vet. Ital, Jan 1, 2009
This study was conducted to produce buffalo embryos in vitro from stepwise vitrified immature ooc... more This study was conducted to produce buffalo embryos in vitro from stepwise vitrified immature oocytes. Cumulus oocyte complexes (COCs) were obtained from the ovaries of slaughtered buffalo and were collected from the local abattoir. Selected COCs were exposed to a vitrification solution consisting of 40% ethylene glycol (EG) plus 0.3 M trehalose and 20% polyvinyl pyrrolidone (PVP) for 1 min and loaded in 0.25 ml plastic mini-straws containing 100 μl of 10% sucrose. The loaded cryostraws were cryopreserved by stepwise vitrification and were stored in liquid nitrogen for 4 to 6 months. Data analysis revealed a high percentage of post-thawing morphologically normal immature oocytes (80.7%) with a low percentage of damaged oocytes. There were no significant differences in the maturation (82.1%), cleavage (47.6%) and buffalo embryo development (15.4%) produced by the stepwise vitrified immature oocytes in comparison to the three observations in fresh oocytes (88.3%, 50.4% and 19.4%, respectively, p<0.05).
Veterinaria italiana, Jan 1, 2009
To improve the efficiency and efficacy of cryopreservation of ovaries, we developed a new method ... more To improve the efficiency and efficacy of cryopreservation of ovaries, we developed a new method termed in situ oocyte (ISO) cryopreservation. ISO cryopreservation is a multistep procedure that involves aspiration of follicular fluid and then perfusion of antral follicles and diffusion of whole buffalo ovaries with cryoprotectant agent (CPA), rapid cooling, storage, thawing and, finally, dilution and removal of the CPA with return to physiological environment. Our study compared ISO cryo ovaries with cryo-diffused ovaries. We systematically examined the effects of ISO cryo and diffuse cryo on ovaries by morphological examination and with viability tests. The percentages of morphologically normal and viable follicular oocytes from ISO cryo were significantly higher than those that resulted from the cryo-diffused method (p<0.01). The quality of follicular oocytes from ISO cryo ovaries appeared better than that achieved from cryo-diffused ovaries. In conclusion, this study shows that ISO cryo is highly efficient for cryopreservation of oocytes and ovarian tissue.
Page 1. INTRODUCTION Royal jelly is secreted by the hypopharyngeal glands of worker bees to feed ... more Page 1. INTRODUCTION Royal jelly is secreted by the hypopharyngeal glands of worker bees to feed young larvae and the adult queen bee. On dry matter basis, royal jelly con-tains considerable amounts of proteins, amino ...
… Insight-An International Journal of Reproduction …, Jan 1, 2011
To improve the efficiency and efficacy of development of buffalo oocytes, we developed a new meth... more To improve the efficiency and efficacy of development of buffalo oocytes, we developed a new method termed mini-straw 'MS'culture system. A novel system of in vitro culture termed the mini-straw culture system is described, mini-straw (French mini-straw, 12.5 cm Length ...
… Insight-An International Journal of Reproduction …, Jan 1, 2011
The present report describes the fertility status of a Cross breed F3 Abondance bull with a novel... more The present report describes the fertility status of a Cross breed F3 Abondance bull with a novel cytogenetic finding: 50% 2n, XY/50% 3n, XY chromosomal complement. We report here on a case of a cross breed Baladi x Abondance (F3 Abondance) male bull with ...
… Insight-An International Journal of Reproduction …, Jan 1, 2012
Data of Holstein dairy cows in a private farm in Cairo Governorate comprising 522 calving events ... more Data of Holstein dairy cows in a private farm in Cairo Governorate comprising 522 calving events (22 for twins and 500 for singles) in the period from December 2009 to December 2010) were used to determine the effect of twinning and length of dry period (DP) on ...
Annales UMCS, Medicina Veterinaria, Jan 1, 2009
Annales UMCS, Medicina Veterinaria, Jan 1, 2010
... susceptibility, cooling rate, diluent composition and osmotic stress) and factors influence f... more ... susceptibility, cooling rate, diluent composition and osmotic stress) and factors influence functional status ... the exposure of spermatozoa to oxidative stress at or just after the thawing ... Procee-dings of the 4th NAAB Technology Conference on Artificial Insemination Reproduction. ...
British Journal of Pharmacology, Jan 1, 2012
Abstract: The present study was conducted to investigate the effects of addition of different con... more Abstract: The present study was conducted to investigate the effects of addition of different concentrations (0.1, 0.2, 0.2, 0.4 and 0.5%, respectively) of Royal Jelly on the viability and maturation of the Egyptian sheep oocytes during in vitro culture of oocytes in serum ...
Buffalo Bulletin (March 2011), Jan 1, 2011
... Saber Mohammed Abd-Allah Theriogenology Department, Faculty of Veterinary Medicine, Beni-Suef... more ... Saber Mohammed Abd-Allah Theriogenology Department, Faculty of Veterinary Medicine, Beni-Suef University, 62511 Beni-Suef,Egypt Original Article Page 2. Buffalo Bulletin (March 2011) Vol.30 No.1 46 occur through simple diffusion. ... al., 1989; McWilliams et al., 1991). ...
Journal of Buffalo Science, Jan 1, 2012
The aim of the present study was to assess the effect of addition of Royal jelly in presence of h... more The aim of the present study was to assess the effect of addition of Royal jelly in presence of heparin on buffalo (Bubalus Bubalis) sperm motility, acrosome reaction and in vitro fertilization (IVF) of buffalo oocytes. Frozen buffalo spermatozoa from five bulls were thawed and motile fraction was obtained by swim up technique. The spermatozoa were washed, treated with100 µg/ml heparin, and then exposed to 0.4% Royal Jelly (RJ) for 3 h. Sperm motility, acrosomal integrity and fertilization rate of matured oocytes were assessed at 1, 2 and 3 h. The percentages of sperm motility, intact acrosome and fertilization rate of matured oocytes were higher (P<0.05) in 0.4% RJ compared to that in the control. After 2 h of incubation the percentage of motility, intact acrosome of spermatozoa and fertilization rate of matured oocytes, respectively, were 93.6 %, 77.6% and 72.6% in 0.4% RJ. These results suggest that treating buffalo sperm with 0.4% RJ in combination with heparin is effective not only to induce sperm acrosome reaction but also is effective for in vitro fertilizing capacity of the cryopreserved buffalo spermatozoa.
Vet. Ital, Jan 1, 2009
This study was conducted to produce buffalo embryos in vitro from stepwise vitrified immature ooc... more This study was conducted to produce buffalo embryos in vitro from stepwise vitrified immature oocytes. Cumulus oocyte complexes (COCs) were obtained from the ovaries of slaughtered buffalo and were collected from the local abattoir. Selected COCs were exposed to a vitrification solution consisting of 40% ethylene glycol (EG) plus 0.3 M trehalose and 20% polyvinyl pyrrolidone (PVP) for 1 min and loaded in 0.25 ml plastic mini-straws containing 100 μl of 10% sucrose. The loaded cryostraws were cryopreserved by stepwise vitrification and were stored in liquid nitrogen for 4 to 6 months. Data analysis revealed a high percentage of post-thawing morphologically normal immature oocytes (80.7%) with a low percentage of damaged oocytes. There were no significant differences in the maturation (82.1%), cleavage (47.6%) and buffalo embryo development (15.4%) produced by the stepwise vitrified immature oocytes in comparison to the three observations in fresh oocytes (88.3%, 50.4% and 19.4%, respectively, p<0.05).
Veterinaria italiana, Jan 1, 2009
To improve the efficiency and efficacy of cryopreservation of ovaries, we developed a new method ... more To improve the efficiency and efficacy of cryopreservation of ovaries, we developed a new method termed in situ oocyte (ISO) cryopreservation. ISO cryopreservation is a multistep procedure that involves aspiration of follicular fluid and then perfusion of antral follicles and diffusion of whole buffalo ovaries with cryoprotectant agent (CPA), rapid cooling, storage, thawing and, finally, dilution and removal of the CPA with return to physiological environment. Our study compared ISO cryo ovaries with cryo-diffused ovaries. We systematically examined the effects of ISO cryo and diffuse cryo on ovaries by morphological examination and with viability tests. The percentages of morphologically normal and viable follicular oocytes from ISO cryo were significantly higher than those that resulted from the cryo-diffused method (p<0.01). The quality of follicular oocytes from ISO cryo ovaries appeared better than that achieved from cryo-diffused ovaries. In conclusion, this study shows that ISO cryo is highly efficient for cryopreservation of oocytes and ovarian tissue.
Page 1. INTRODUCTION Royal jelly is secreted by the hypopharyngeal glands of worker bees to feed ... more Page 1. INTRODUCTION Royal jelly is secreted by the hypopharyngeal glands of worker bees to feed young larvae and the adult queen bee. On dry matter basis, royal jelly con-tains considerable amounts of proteins, amino ...
… Insight-An International Journal of Reproduction …, Jan 1, 2011
To improve the efficiency and efficacy of development of buffalo oocytes, we developed a new meth... more To improve the efficiency and efficacy of development of buffalo oocytes, we developed a new method termed mini-straw 'MS'culture system. A novel system of in vitro culture termed the mini-straw culture system is described, mini-straw (French mini-straw, 12.5 cm Length ...
… Insight-An International Journal of Reproduction …, Jan 1, 2011
The present report describes the fertility status of a Cross breed F3 Abondance bull with a novel... more The present report describes the fertility status of a Cross breed F3 Abondance bull with a novel cytogenetic finding: 50% 2n, XY/50% 3n, XY chromosomal complement. We report here on a case of a cross breed Baladi x Abondance (F3 Abondance) male bull with ...
… Insight-An International Journal of Reproduction …, Jan 1, 2012
Data of Holstein dairy cows in a private farm in Cairo Governorate comprising 522 calving events ... more Data of Holstein dairy cows in a private farm in Cairo Governorate comprising 522 calving events (22 for twins and 500 for singles) in the period from December 2009 to December 2010) were used to determine the effect of twinning and length of dry period (DP) on ...
Annales UMCS, Medicina Veterinaria, Jan 1, 2009
Annales UMCS, Medicina Veterinaria, Jan 1, 2010
... susceptibility, cooling rate, diluent composition and osmotic stress) and factors influence f... more ... susceptibility, cooling rate, diluent composition and osmotic stress) and factors influence functional status ... the exposure of spermatozoa to oxidative stress at or just after the thawing ... Procee-dings of the 4th NAAB Technology Conference on Artificial Insemination Reproduction. ...