syed azimuddin | Chinese Academy of Sciences (original) (raw)

Papers by syed azimuddin

Under salt and toxic metal stress condition, genetic make-up and proteins including some enzymes ... more Under salt and toxic metal stress condition, genetic make-up and proteins including some enzymes undergo specific changes to combat the posed harsh environment. Carbonic anhydrase is ubiquitous enzyme associated with respiratory disorder and cancerous condition. Strategies investigated and adopted by microorganisms to cope with hypersaline by bioinformatics analysis including homology modeling, Zinc metal interaction prediction, sequence analysis for Carbonic Anhydrase. This study shows that carbonic anhydrase acquired more acidic residues on its surface as countermeasure and may form salt bridges with positive ions in response to hypersaline environment. It also reduced its surface area and had more polar residues. Zinc metal interaction with Nitrogen of Histidine residues were almost conserved in the studied and modelled structure with fewer changes in catalytic region of sequence pattern. Salt tolerance achieved by foster promising approaches at the genetic and proteomic level by...

Klebsiella pneumoniae is the causative agent of numerous types of infections in humans, includes ... more Klebsiella pneumoniae is the causative agent of numerous types of infections in humans, includes urinary tract infections (UTIs), respiratory tract infections, and bloodstream infections. The hypervirulent Klebsiella pneumoniae associated with severe community-acquired infections. Hypervirulent Klebsiella pneumoniae(hvKP) is more virulent than classical Klebsiella pneumoniae (cKP) and it is an evolving pathotype due to the acquisition of genetic determinants which is responsible for its open genome. In the present study, we have performed the pan-genome analysis of Klebsiella pneumoniae for the determination of virulence proteins and genomic variation among its strains using a tool pangenome analysis pipeline (PGAP-1.2.1). The whole genome sequences of fifty-one Klebsiella pneumoniae strains was downloaded from NCBI using Refseq database. These sequences were converted into PGAP required extension files. Thirty-nine proteins and their corresponding protein sequences involved in the ...

Mycoplasma pneumoniae causes respiratory infections and other mucous membrane related inflammatio... more Mycoplasma pneumoniae causes respiratory infections and other mucous membrane related inflammations. To explore the intra-specific variations and evolution of M. pneumoniae, a pan-genomic analysis was performed on 150 strains. In genome size evaluation of M. pneumoniae, on average 687 genes with low S.D (8.43) showed overall consistency in the gene count of 150 strains. This species is potentially pathogenic and highly evolving as 134 out of 150 showed almost all pathogenic factors with above 90% homology except 16 strains which belong to Spain, USA, China, and Japan. M. pneumoniae pan genome is an open pan genome showed total genes of 103174 in which 70359 (68.19%) core genes, 32241(31.24%) shared genes and 574 (0.55%) unique genes. Adhesin P30, Protein TopJ, ADP-ribosylating toxin CARDS toxin, GLPF, GLPK, GLPO, GLT and IgG-blocking protein M are the part of core genome. Adhesin P1, HMW1 and HMW2 genes are the part of shared genome. HMW3 and Cytadherence-associated protein P65 are ...

File names are self explanatory. The data set was created by PGAP Refer the manual and the paper ... more File names are self explanatory. The data set was created by PGAP Refer the manual and the paper 10.1093/bioinformatics/btr655 https://sourceforge.net/projects/pgap/

The data set contains balst hit files for the results obtained by online balst of mer genes for t... more The data set contains balst hit files for the results obtained by online balst of mer genes for the refseq database and customized database of 49 strains created by makeblastdb

The scripts meant for data probing and processing of genomic data and blastp results

potassium phosphate buffer containing EDTA Lactate dehydrogenase was purified 21-fold from liver ... more potassium phosphate buffer containing EDTA Lactate dehydrogenase was purified 21-fold from liver of Varanus bengalensis using colchicine-sepharose column chromatography. The crude enzyme showed two isoenzymes (LDH-5 and LDH-4) by agarose gel electrophoresis (AGE). The p u r i f i e d enzyme showed a single band after SDS-PAGE corresponding to molecular mass of 35 kDa. The molecular mass of native enzyme was about 140 kDa. The optimum pH for the forward reaction was 7.5 while that for the reverse reaction was pH 9.5. The Km values for pyruvate, NADH, lactate and NAD+were 0.17 ± 0.037, 0.02 ± 0.004, 12.4 ± 3.05 and 0.38 ± 0.032 mM, respectively. Pre-heating of enzyme showed that its t5 0 was 40-50˚C. Oxalate and n-hexanediol were inhibitors for both forward and reverse reactions. Among divalent ions, Cu+ + was shown to be more effective inhibitor for the forward reaction. K e y w o r d s:LDH isoenzyme, liver, properties, purification, Varanus bengalensis

Nipple discharge is one of the most common breast complaints in women. Study was designed to see ... more Nipple discharge is one of the most common breast complaints in women. Study was designed to see the variation in biochemical parameters like carcino-embryonic antigen (CEA), Lactate dehydrogenase (LDH), cholesterol, triglyceride and occult blood in malignant and non-malignant discharge. Besides, the effect of allopathic/homeopathic drug on malignant nipple discharge was also studied. Color of normal fluid was milky white and of malignant was dark brown. Levels of CEA and LDH were markedly increased in malignant discharge than in non-malignant. In contrast, the level of protein, cholesterol and triglyceride were decreased in malignant discharge as compared to non-malignant. Incubation of drugs with malignant fluid shows no marked electrophoretic changes in protein pattern. On the other hand, incubated mixture run on reverse phase HPLC showed that tamoxifen is most effective than methotrixate. Results showed that analysis of nipple discharge may help in the diagnosis and treatment of...

Nipple discharge is one of the most common breast complaints in women. Study was designed to see ... more Nipple discharge is one of the most common breast complaints in women. Study was designed to see the variation in biochemical parameters like carcino-embryonic antigen (CEA), Lactate dehydrogenase (LDH), cholesterol, triglyceride and occult blood in malignant and nonmalignant discharge. Besides, the effect of allopathic/homeopathic drug on malignant nipple discharge was also studied. Color of normal fluid was milky white and of malignant was dark brown. Levels of CEA and LDH were markedly increased in malignant discharge than in non-malignant. In contrast, the level of protein, cholesterol and triglyceride were decreased in malignant discharge as compared to nonmalignant. Incubation of drugs with malignant fluid shows no marked electrophoretic changes in protein pattern. On the other hand, incubated mixture run on reverse phase HPLC showed that tamoxifen is most effective than methotrixate. Results showed that analysis of nipple discharge may help in the diagnosis and treatment of breast cancer. Hence, further research is needed on large number of samples to reach a definite conclusion.

Lactate dehydrogenase was purified 21-fold from liver of Varanus bengalensis using colchicineseph... more Lactate dehydrogenase was purified 21-fold from liver of Varanus bengalensis using colchicinesepharose column chromatography. The crude enzyme showed two isoenzymes (LDH-5 and LDH-4 ) by agarose gel electrophoresis (AGE). The p u r i f i e d enzyme showed a single band after SDS-PAGE corresponding to molecular mass of 35 kDa. The molecular mass of native enzyme was about 140 kDa. The optimum pH for the forward reaction was 7.5 while that for the reverse reaction was pH 9.5. The K m values for pyruvate, NADH, lactate and NAD + were 0.17 ± 0.037, 0.02 ± 0.004, 12.4 ± 3.05 and 0.38 ± 0.032 mM, respectively. Pre-heating of enzyme showed that its t 5 0 was 40-50˚C. Oxalate and n-hexanediol were inhibitors for both forward and reverse reactions. Among divalent ions, Cu + + was shown to be more effective inhibitor for the forward reaction. K e y w o r d s : LDH isoenzyme, liver, properties, purification, Varanus bengalensis

The scripts meant for data probing and processing of genomic data and blastp results

Nipple discharge is one of the most common breast complaints in women. Study was designed to see ... more Nipple discharge is one of the most common breast complaints in women. Study was designed to see the variation in biochemical parameters like carcino-embryonic antigen (CEA), Lactate dehydrogenase (LDH), cholesterol, triglyceride and occult blood in malignant and nonmalignant discharge. Besides, the effect of allopathic/homeopathic drug on malignant nipple discharge was also studied. Color of normal fluid was milky white and of malignant was dark brown. Levels of CEA and LDH were markedly increased in malignant discharge than in non-malignant. In contrast, the level of protein, cholesterol and triglyceride were decreased in malignant discharge as compared to nonmalignant. Incubation of drugs with malignant fluid shows no marked electrophoretic changes in protein pattern. On the other hand, incubated mixture run on reverse phase HPLC showed that tamoxifen is most effective than methotrixate. Results showed that analysis of nipple discharge may help in the diagnosis and treatment of breast cancer. Hence, further research is needed on large number of samples to reach a definite conclusion.