Dr.Tathagat Waghmare | Gulbarga University (original) (raw)

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Papers by Dr.Tathagat Waghmare

Research paper thumbnail of Microbial Production of 1, 3-Propanediol using Newly Isolated Enterobacter cloacae GNTEW13-1, GNTEW13-V and its Enhancement Studies by UV Mutagenesis

The crude glycerol has been converted to 1, 3-propanediol which is not only a useful final produc... more The crude glycerol has been converted to 1, 3-propanediol which is not only a useful final product but also a valuable starting compound for producing polymers, A newly isolated Enterobacter cloacae GNTEW13-1 and GNTEW13-V has shown a high produciblility of 1, 3-propanediol by using biofuel derived crude glycerol as a sole carbon and energy source. In the preliminary stage the Enterobacter cloacae GNTEW13-1 and GNTEW13-V has grown on the glycerol based medium to enhance the more utilization of glycerol for the high production 1, 3-propanediol. Total 10 mutants were screened after UV Treatment and among these mutants, 3 mutants showed increased production over parent strains.

Research paper thumbnail of Isolation, screening and characterization of crude glycerol utilizing microorganism for the production of 1, 3-propanediol

Rapidly Expanding market for bio-diesel has increased the supply and eventually decreased the cos... more Rapidly Expanding market for bio-diesel has increased the supply and eventually decreased the cost of crude glycerol, which makes an attractive sustainable feed stock for the fuel and chemical Industry. The conversion of glycerol may be carried out by both chemical as well as biological means.
Many microorganisms are known to convert glycerol to value added industrially important products. The present study involved the isolation of potent bacterial strains from soil sample obtained from bio-diesel information and demonstration center, Gulbarga University, Gulbarga, Karnataka, India. The isolated strain was Enterobacter safensis and is designated as GNTEW-13. As the Enterobacter safensis strain is the known producer of 1,3-PDO, is an important component of fuel and polyesters (used widely in the petroleum industry) using bio-diesel derived crude glycerol for producing 1,3- Propanediol, which is a substituent in various industrial sectors as good and remarkable solution from an commercial as well as ecological point of view.

Research paper thumbnail of QUANTITATIVE ESTIMATION OF HEAVY METALS IN RIVER WATER AND THEIR TOXICITY, HEMATOLOGY, GRAVIMETRIC, SERUM AND TISSUE BIOCHEMISTRY EFFECTS IN ALBINO RATS

ABSTRACT The aim of present study is to investigate on the concentration of heavy metals (Cu, Ni,... more ABSTRACT
The aim of present study is to investigate on the concentration of heavy
metals (Cu, Ni, Fe, Co, Mn, Cr and Zn) present in the water and their
effects on Toxicity, Hematology, Gravimetric and Serum biochemistry
of healthy albino rats. The results showed that 50% of toxicity and
there was significant difference (p<0.05) in the Red Blood Cell (RBC)
and White Blood Cell (WBC), It indicate the hemolytic anemia. This is
due to injurious effect of the toxic heavy metals of water. Where in
case of Gravimetric study the average weight reduced significantly
(p<0.05) in test rats when compared with control, indicating that, the
excess heavy metal water exposure to rats caused reduction in weight
of liver, heart, kidney and adrenal. The serum biochemistry studies of
protein, cholesterol, urea, and phospholipids were reduced in all the
groups studied. Thus, it can be concluded that heavy metals of water as serious consequences
on toxicity, hematology, Gravimetric, Tissue and serum biochemistry in rats and causes
hemolytic anemia which is attributed to the toxic effect of heavy metals of the water in the
experimental rats.

Research paper thumbnail of CHEMICAL ANALYSIS OF SEED AND OIL OF JATROPHA CURCUS L’ A BIOFUEL PLANT CULTIVATED IN HYDERABAD KARNATAKA REGION, NORTH KARNATAKA.

ABSTRACT: Jatropha curcas L. is a multipurpose shrub with a variety of applications and enormous ... more ABSTRACT: Jatropha curcas L. is a multipurpose shrub with a variety of applications and enormous economic
potentials for its seed oil, which can be converted into biodiesel- an alternative to petro-diesel. It aims to overcome
energy crisis problem and also to reduce environmental changes. The fact that the oil of J. curcas cannot be used for
nutritional purposes without detoxification makes its use as an energy source for fuel production very attractive. Oil
content of Jatropha curcus L was subjected to various physicochemical parameters. The chemical parameters include
determination of free fatty acids, peroxide value, Iodine value, saponification value, various physiological analysis of
Jatropha curcus L plant was also carried out in order to estimate the presence of total content of chlorophyll. By
considering all the above parameters the present study reveals that the Jatropha curcus L l plant is good for production
of better quality of biodiesel.

Research paper thumbnail of Study of physicochemical and hematological effects of Bheema river water on Albino rat (Rattus norvegicus)

The present study was designed to investigate the quality of Bheema river water on its potability... more The present study was designed to investigate the quality of Bheema river water on its potability and haematological parameters on albino rats. The rats were divided into four groups and were fed with Bheema river water subsequently for one day, four day and seven day. The rats of control group received only normal tap water, where as group one, group two, and group three were fed with Bheema river water for one day four day and seven day. The rats treated for two days have shown significant reduction in the Hemoglobin content and, differential counts compared to other group. Simultaneously we have checked the various physical and chemical parameters of Bheema river water where we observed significant variations in the chemical analysis of the water quality compare to normal water which shows that the Bheema river water is polluted and not good for drinking purpose.

Research paper thumbnail of Antimicrobial Properties of the Methanolic Extracts of Zingiber officinale (Ginger) on Escherichia coli and Klebsiella pneumoniae

The emerging trends of multidrug resistance among several groups of microorganisms against differ... more The emerging trends of multidrug resistance among several groups of microorganisms against different classes of antibiotics led different researchers to develop efficient drugs from various plant sources to counter multidrug resistant strains. This study investigated different solvent extracts of Zingiber officinale, to determine their efficacy against multidrug resistant microbes. The antimicrobial properties of various extract of Zingiber officinale (ginger) against Escherichia coli and Klebsiella pneumoniae that are common causes of gastrointestinal tract infection were investigated using the Agar and tube diffusion method. The results obtained revealed that Methanolic extract of ginger gave the widest zone of inhibition against one out of the two test organisms at the concentration of 20 mg mL -1 . However, Klebsiella pneumonia was more sensitive to the extract. It was also observed that the solvent of extraction and its varying concentrations affected the sensitivity of the two test organisms to the plant extract showing that ginger has antimicrobial activities on the test organisms due to its inhibitory effect, thus confirming its use in folk medicine.

Research paper thumbnail of Antimicrobial Properties of the Methanolic Extracts of Zingiber officinale (Ginger) on Escherichia coli and Klebsiella pneumoniae

The emerging trends of multidrug resistance among several groups of microorganisms against differ... more The emerging trends of multidrug resistance among several groups of microorganisms against different classes of antibiotics led different researchers to develop efficient drugs from various plant sources to counter multidrug resistant strains. This study investigated different solvent extracts of Zingiber officinale, to determine their efficacy against multidrug resistant microbes. The antimicrobial properties of various extract of Zingiber officinale (ginger) against Escherichia coli and Klebsiella pneumoniae that are common causes of gastrointestinal tract infection were investigated using the Agar and tube diffusion method. The results obtained revealed that Methanolic extract of ginger gave the widest zone of inhibition against one out of the two test organisms at the concentration of 20 mg mL-1. However, Klebsiella pneumonia was more sensitive to the extract. It was also observed that the solvent of extraction and its varying concentrations affected the sensitivity of the two test organisms to the plant extract showing that ginger has antimicrobial activities on the test organisms due to its inhibitory effect, thus confirming its use in folk medicine.

Conference Presentations by Dr.Tathagat Waghmare

Research paper thumbnail of A novel microbial strains for producing 1,3-propanediol (Bio-plastic) from bio-diesel derived glycerol as a sole carbon source

Rapidly Expanding market for bio-diesel has increased the supply and eventually decreased the co... more Rapidly Expanding market for bio-diesel has increased the supply and eventually decreased the cost of crude glycerol, which makes an attractive sustainable feed stock for the fuel and chemical Industry. The conversion of glycerol may be carried out by both chemical as well as biological means. Many microorganisms are known to convert glycerol to value added industrially important products. The present study involved the isolation of potent bacterial strains from soil sample obtained from soil sample. The isolated strain was Enterobacter cloacae. As the Enterobacter cloacae strain is the known producer of 1,3-PDO (Bioplastic), is an important component of fuel and polyesters (used widely in the petroleum industry) using biodiesel derived crude glycerol for producing 1,3-Propanediol, which is a substituent in various industrial sectors as good and remarkable solution from an commercial as well as ecological point of view. The crude glycerol has been converted to 1, 3-propanediol which is not only a useful final product but also a valuable starting compound for producing polymers, A newly isolated Enterobacter cloacae-S2-5, Enterobacter cloacae GNTEW13-1 and Enterobacter cloacae GNTEW13-V has shown a high produciblility of 1, 3-propanediol by using biofuel derived crude glycerol as a sole carbon and energy source. In the preliminary stage Isolation, Identification and Characterization of potent 1,3-Propanediol producing microbial strain the Enterobacter cloacae-S2-5, Enterobacter cloacae GNTEW13-1 and GNTEW13-V has been carried out and checked their ability utilizing crude glycerol as a sole carbon source and produce industrially important chemical constituent such as 1,3-Propanediol at laboratory scale. And further the strain Enterobacter cloacae-S2-5, Enterobacter cloacae GNTEW13-1 and GNTEW13-V have been optimized for the enhancement of highest 1,3-propanediol production rate.

Research paper thumbnail of Microbial Production of 1, 3-Propanediol using Newly Isolated Enterobacter cloacae GNTEW13-1, GNTEW13-V and its Enhancement Studies by UV Mutagenesis

The crude glycerol has been converted to 1, 3-propanediol which is not only a useful final produc... more The crude glycerol has been converted to 1, 3-propanediol which is not only a useful final product but also a valuable starting compound for producing polymers, A newly isolated Enterobacter cloacae GNTEW13-1 and GNTEW13-V has shown a high produciblility of 1, 3-propanediol by using biofuel derived crude glycerol as a sole carbon and energy source. In the preliminary stage the Enterobacter cloacae GNTEW13-1 and GNTEW13-V has grown on the glycerol based medium to enhance the more utilization of glycerol for the high production 1, 3-propanediol. Total 10 mutants were screened after UV Treatment and among these mutants, 3 mutants showed increased production over parent strains.

Research paper thumbnail of Isolation, screening and characterization of crude glycerol utilizing microorganism for the production of 1, 3-propanediol

Rapidly Expanding market for bio-diesel has increased the supply and eventually decreased the cos... more Rapidly Expanding market for bio-diesel has increased the supply and eventually decreased the cost of crude glycerol, which makes an attractive sustainable feed stock for the fuel and chemical Industry. The conversion of glycerol may be carried out by both chemical as well as biological means.
Many microorganisms are known to convert glycerol to value added industrially important products. The present study involved the isolation of potent bacterial strains from soil sample obtained from bio-diesel information and demonstration center, Gulbarga University, Gulbarga, Karnataka, India. The isolated strain was Enterobacter safensis and is designated as GNTEW-13. As the Enterobacter safensis strain is the known producer of 1,3-PDO, is an important component of fuel and polyesters (used widely in the petroleum industry) using bio-diesel derived crude glycerol for producing 1,3- Propanediol, which is a substituent in various industrial sectors as good and remarkable solution from an commercial as well as ecological point of view.

Research paper thumbnail of QUANTITATIVE ESTIMATION OF HEAVY METALS IN RIVER WATER AND THEIR TOXICITY, HEMATOLOGY, GRAVIMETRIC, SERUM AND TISSUE BIOCHEMISTRY EFFECTS IN ALBINO RATS

ABSTRACT The aim of present study is to investigate on the concentration of heavy metals (Cu, Ni,... more ABSTRACT
The aim of present study is to investigate on the concentration of heavy
metals (Cu, Ni, Fe, Co, Mn, Cr and Zn) present in the water and their
effects on Toxicity, Hematology, Gravimetric and Serum biochemistry
of healthy albino rats. The results showed that 50% of toxicity and
there was significant difference (p<0.05) in the Red Blood Cell (RBC)
and White Blood Cell (WBC), It indicate the hemolytic anemia. This is
due to injurious effect of the toxic heavy metals of water. Where in
case of Gravimetric study the average weight reduced significantly
(p<0.05) in test rats when compared with control, indicating that, the
excess heavy metal water exposure to rats caused reduction in weight
of liver, heart, kidney and adrenal. The serum biochemistry studies of
protein, cholesterol, urea, and phospholipids were reduced in all the
groups studied. Thus, it can be concluded that heavy metals of water as serious consequences
on toxicity, hematology, Gravimetric, Tissue and serum biochemistry in rats and causes
hemolytic anemia which is attributed to the toxic effect of heavy metals of the water in the
experimental rats.

Research paper thumbnail of CHEMICAL ANALYSIS OF SEED AND OIL OF JATROPHA CURCUS L’ A BIOFUEL PLANT CULTIVATED IN HYDERABAD KARNATAKA REGION, NORTH KARNATAKA.

ABSTRACT: Jatropha curcas L. is a multipurpose shrub with a variety of applications and enormous ... more ABSTRACT: Jatropha curcas L. is a multipurpose shrub with a variety of applications and enormous economic
potentials for its seed oil, which can be converted into biodiesel- an alternative to petro-diesel. It aims to overcome
energy crisis problem and also to reduce environmental changes. The fact that the oil of J. curcas cannot be used for
nutritional purposes without detoxification makes its use as an energy source for fuel production very attractive. Oil
content of Jatropha curcus L was subjected to various physicochemical parameters. The chemical parameters include
determination of free fatty acids, peroxide value, Iodine value, saponification value, various physiological analysis of
Jatropha curcus L plant was also carried out in order to estimate the presence of total content of chlorophyll. By
considering all the above parameters the present study reveals that the Jatropha curcus L l plant is good for production
of better quality of biodiesel.

Research paper thumbnail of Study of physicochemical and hematological effects of Bheema river water on Albino rat (Rattus norvegicus)

The present study was designed to investigate the quality of Bheema river water on its potability... more The present study was designed to investigate the quality of Bheema river water on its potability and haematological parameters on albino rats. The rats were divided into four groups and were fed with Bheema river water subsequently for one day, four day and seven day. The rats of control group received only normal tap water, where as group one, group two, and group three were fed with Bheema river water for one day four day and seven day. The rats treated for two days have shown significant reduction in the Hemoglobin content and, differential counts compared to other group. Simultaneously we have checked the various physical and chemical parameters of Bheema river water where we observed significant variations in the chemical analysis of the water quality compare to normal water which shows that the Bheema river water is polluted and not good for drinking purpose.

Research paper thumbnail of Antimicrobial Properties of the Methanolic Extracts of Zingiber officinale (Ginger) on Escherichia coli and Klebsiella pneumoniae

The emerging trends of multidrug resistance among several groups of microorganisms against differ... more The emerging trends of multidrug resistance among several groups of microorganisms against different classes of antibiotics led different researchers to develop efficient drugs from various plant sources to counter multidrug resistant strains. This study investigated different solvent extracts of Zingiber officinale, to determine their efficacy against multidrug resistant microbes. The antimicrobial properties of various extract of Zingiber officinale (ginger) against Escherichia coli and Klebsiella pneumoniae that are common causes of gastrointestinal tract infection were investigated using the Agar and tube diffusion method. The results obtained revealed that Methanolic extract of ginger gave the widest zone of inhibition against one out of the two test organisms at the concentration of 20 mg mL -1 . However, Klebsiella pneumonia was more sensitive to the extract. It was also observed that the solvent of extraction and its varying concentrations affected the sensitivity of the two test organisms to the plant extract showing that ginger has antimicrobial activities on the test organisms due to its inhibitory effect, thus confirming its use in folk medicine.

Research paper thumbnail of Antimicrobial Properties of the Methanolic Extracts of Zingiber officinale (Ginger) on Escherichia coli and Klebsiella pneumoniae

The emerging trends of multidrug resistance among several groups of microorganisms against differ... more The emerging trends of multidrug resistance among several groups of microorganisms against different classes of antibiotics led different researchers to develop efficient drugs from various plant sources to counter multidrug resistant strains. This study investigated different solvent extracts of Zingiber officinale, to determine their efficacy against multidrug resistant microbes. The antimicrobial properties of various extract of Zingiber officinale (ginger) against Escherichia coli and Klebsiella pneumoniae that are common causes of gastrointestinal tract infection were investigated using the Agar and tube diffusion method. The results obtained revealed that Methanolic extract of ginger gave the widest zone of inhibition against one out of the two test organisms at the concentration of 20 mg mL-1. However, Klebsiella pneumonia was more sensitive to the extract. It was also observed that the solvent of extraction and its varying concentrations affected the sensitivity of the two test organisms to the plant extract showing that ginger has antimicrobial activities on the test organisms due to its inhibitory effect, thus confirming its use in folk medicine.

Research paper thumbnail of A novel microbial strains for producing 1,3-propanediol (Bio-plastic) from bio-diesel derived glycerol as a sole carbon source

Rapidly Expanding market for bio-diesel has increased the supply and eventually decreased the co... more Rapidly Expanding market for bio-diesel has increased the supply and eventually decreased the cost of crude glycerol, which makes an attractive sustainable feed stock for the fuel and chemical Industry. The conversion of glycerol may be carried out by both chemical as well as biological means. Many microorganisms are known to convert glycerol to value added industrially important products. The present study involved the isolation of potent bacterial strains from soil sample obtained from soil sample. The isolated strain was Enterobacter cloacae. As the Enterobacter cloacae strain is the known producer of 1,3-PDO (Bioplastic), is an important component of fuel and polyesters (used widely in the petroleum industry) using biodiesel derived crude glycerol for producing 1,3-Propanediol, which is a substituent in various industrial sectors as good and remarkable solution from an commercial as well as ecological point of view. The crude glycerol has been converted to 1, 3-propanediol which is not only a useful final product but also a valuable starting compound for producing polymers, A newly isolated Enterobacter cloacae-S2-5, Enterobacter cloacae GNTEW13-1 and Enterobacter cloacae GNTEW13-V has shown a high produciblility of 1, 3-propanediol by using biofuel derived crude glycerol as a sole carbon and energy source. In the preliminary stage Isolation, Identification and Characterization of potent 1,3-Propanediol producing microbial strain the Enterobacter cloacae-S2-5, Enterobacter cloacae GNTEW13-1 and GNTEW13-V has been carried out and checked their ability utilizing crude glycerol as a sole carbon source and produce industrially important chemical constituent such as 1,3-Propanediol at laboratory scale. And further the strain Enterobacter cloacae-S2-5, Enterobacter cloacae GNTEW13-1 and GNTEW13-V have been optimized for the enhancement of highest 1,3-propanediol production rate.