yash gadhikar | Rashtrasant Tukdoji Maharaj Nagpur University, Nagpur (original) (raw)

Papers by yash gadhikar

Research paper thumbnail of Changes in NPY gene expression during exogenus starvation in H. fossilis

International Journal of Fisheries and Aquatic Studies, 2017

Starvation condition is experienced in many fish species every year, due to various environmental... more Starvation condition is experienced in many fish species every year, due to various environmental conditions. Also, it is evident that starvation results in mortality. Metabolic enzymes, RNA/DNA ratio and protein also get affected during fasting. We aimed to investigate the distribution of endocrine cell immunoreactivity for the neuropeptide-Y (NP-Y) in stomach and intestinal region of digestive tract of teleost cat fish Heteropneustes fossilis, by using immunocytochemistry. The present study also focused to access the effect of starvation on mRNA gene expression in the intestine of the fish. The results revealed the NP-Y immunoreactive endocrine cells from the intestine of Heteropneustes fossilis were quite varied as compared to other teleost fish species. Stomach showed complete absence of immunoreactivity. mRNA concentration increased significantly in middle region due to increased in starvation period. Above findings confirmed the role of NPY in regulation of feeding and digestion.

Research paper thumbnail of Immunocytochemical Localization of Ubiquitin in Developing Occipital and Prefrontal Cortex of Human Fetal Brain During Second Trimester

Ubiquitin immunoreactive profiles were studied in developing human fetal occipital cortex and the... more Ubiquitin immunoreactive profiles were studied in developing human fetal occipital cortex and the prefrontal cortex during the second trimester. We employed immunolabelling techniques on seven human fetal occipital cortices and prefrontal cortices ranging from 12 weeks gestation (w.g.) to 25w.g. by using ubiquitin antibody. At the end of first trimester, immunoreactive profiles were relatively meager in both the areas. In 12w.g. moderate to intense ubiquitin immunoreactivity was detected in the marginal zone (MZ) and ventricular zone (VZ), where as fibrous layer at this stage showed diffuse nonspecific immunoreactivity. At 17 weeks occipital and prefrontal cortex, staining was restricted to the cells while at 18, 19, 20w.g. immunoreactive product was observed in the fibrous network. At 22w.g. ubiquitin immunoreactivity was detected in the fibrous network of occipital cortex while wide spaces were detected in the fibrous layer of prefrontal cortex. With the increase in fetal age i.e....

Research paper thumbnail of ASSESSMENT OF PHYTOCHEMICAL COMPOSITION AND ANTIBACTERIAL ACTIVITY OF DIFFERENT EXTRACTS OF Barleria prionitis LEAVES AGAINST ORAL MICROFLORA TO IMPROVE DENTAL HYGIENE

Oral diseases are major health problem with dental caries. Oral health influence the general qual... more Oral diseases are major health problem with dental caries. Oral health influence the general quality of life and poor oral health is linked to chronic conditions and systemic diseases. Hence there is an urgent need to find out an alternative way, instead of antibiotic and chemicals for the treatment of oral diseases which show more side effects. Present investigation is focused on antibacterial activity of aqueous, petroleum ether, chloroform and acetone extract of leaves of Barleria prionitis (Family-Acanthaceae) against seven bacterial species (Lactobacillus rhamnosus, Staphylococcus aureus , Bacillus subtilis , Actinomyces viscoscus, Staphylococcus epidermidis, Escherichia coli , Streptococcus mutans ) which are known to cause oral diseases in human being. Similarly leaves of Barleria prionitis were evaluated for its phytochemical composition. Antibacterial activity of the extracts was determined by paper disc diffusion method using 200mg/ml concentration of extract and there % z...

Research paper thumbnail of Histological and Histochemical Study on the Stomach and Intestine of Catfish Heteropneustes Fossilis (Bloch 1794)

The present work describes the histological and histochemical studies of stomach and intestine of... more The present work describes the histological and histochemical studies of stomach and intestine of Heteropneustes fossilis. The stomach and intestinal morphology is influenced by its feeding habits. The histological studies of stomach and intestine of H. fossilis revealed the basic pattern of wall layers as that other teleost fishes and vertebrates. However, few variations were observed in the thickness of muscle layers in the pyloric regions as compared to cardio-fundic region, which may be due to the variation in feeding habits. The stomach of H. fossilis consists of two parts cardio-fundic and pyloric. Mucosa layer of cardio-fundic region formed by numerous tapered fold covered by the columnar epithelium. The gastric glands are more in cardio-fundic region while rare in the pyloric region. Histochemically all the mucous secreting cells of the stomach are positive to the PAS stain and negative to the AB stain. Intestine of H. fossilis is long, coiled and divided into three parts; p...

Research paper thumbnail of A Preliminary checklist of Moths species from Amravati city, Maharashtra (India)

Present study was an attempt to explore moth diversity from different parts of the Amravati city.... more Present study was an attempt to explore moth diversity from different parts of the Amravati city. A total number of 628 moths were identified up to the family level. Families Sphingidae, Noctuidae, Geometridae, Crambidae, Arctiidae, Lymantiidae and Saturnidae were represented in the identified samples. The diversity index of Noctuidae family 2.63 was high as compared to the other families. While the lowest diversity index was found in family Geometridae and Saturnidae.

Research paper thumbnail of Asian Journal Of Biology and Biotechnology 2014 Histology, histochemical and ultrastructural studies on Intestine of Indian catfish, Clarias batrachus (Linn 1758)

Research paper thumbnail of Bioremediation of industrial waste by using bat guano

Journal of environmental science & engineering, 2007

The present investigation is an attempt to study the effect of bat guano with its rich microbial ... more The present investigation is an attempt to study the effect of bat guano with its rich microbial flora on bioremediation of industrial waste effluents. The results revealed that within a period of 15 days, there was a remarkable reduction in the Chemical Oxygen Demand (COD) values up to 50%-70%, thus stabilizing the industrial effluents. In addition to this,values of various physico-chemical parameters were notably found to reduce suggesting that industrial effluents can be effectively treated by bat guano.

Research paper thumbnail of A Preliminary Report on the Moths (Insecta: Lepidoptera: Heterocera) Fauna from Amravati, Maharashtra

Moths belong to the order Lepidoptera and this type of fauna are easily affected by a slightest c... more Moths belong to the order Lepidoptera and this type of fauna are easily affected by a slightest change in environment so keeping in view moths could be used to check the minute change of environment hence can be called as bioindicators of environment. Taking a chance to investigate environmental health all possible efforts were carried out in this work to list and unfold this hidden fauna of city and some surrounding areas. Collection of moths was carried out from August 2012 to January 2013 to determine their diversity and occurance. A total number of 41 moth specimens were collected by using simple light traps operated from dusk to dawn. The moths were identified up to family level. Families Noctuidae, Arctiidae, Geometridae, Sphingdae, Saturniidae, Crambidae, Lasiocampidae and Lymantridae were presented in collection samples. Keyword: Abundance, collection, diversity, identification, moth taxonomy.

Research paper thumbnail of Changes in NPY gene expression during exogenus starvation in H. fossilis

International Journal of Fisheries and Aquatic Studies, 2017

Starvation condition is experienced in many fish species every year, due to various environmental... more Starvation condition is experienced in many fish species every year, due to various environmental conditions. Also, it is evident that starvation results in mortality. Metabolic enzymes, RNA/DNA ratio and protein also get affected during fasting. We aimed to investigate the distribution of endocrine cell immunoreactivity for the neuropeptide-Y (NP-Y) in stomach and intestinal region of digestive tract of teleost cat fish Heteropneustes fossilis, by using immunocytochemistry. The present study also focused to access the effect of starvation on mRNA gene expression in the intestine of the fish. The results revealed the NP-Y immunoreactive endocrine cells from the intestine of Heteropneustes fossilis were quite varied as compared to other teleost fish species. Stomach showed complete absence of immunoreactivity. mRNA concentration increased significantly in middle region due to increased in starvation period. Above findings confirmed the role of NPY in regulation of feeding and digestion.

Research paper thumbnail of Immunocytochemical Localization of Ubiquitin in Developing Occipital and Prefrontal Cortex of Human Fetal Brain During Second Trimester

Ubiquitin immunoreactive profiles were studied in developing human fetal occipital cortex and the... more Ubiquitin immunoreactive profiles were studied in developing human fetal occipital cortex and the prefrontal cortex during the second trimester. We employed immunolabelling techniques on seven human fetal occipital cortices and prefrontal cortices ranging from 12 weeks gestation (w.g.) to 25w.g. by using ubiquitin antibody. At the end of first trimester, immunoreactive profiles were relatively meager in both the areas. In 12w.g. moderate to intense ubiquitin immunoreactivity was detected in the marginal zone (MZ) and ventricular zone (VZ), where as fibrous layer at this stage showed diffuse nonspecific immunoreactivity. At 17 weeks occipital and prefrontal cortex, staining was restricted to the cells while at 18, 19, 20w.g. immunoreactive product was observed in the fibrous network. At 22w.g. ubiquitin immunoreactivity was detected in the fibrous network of occipital cortex while wide spaces were detected in the fibrous layer of prefrontal cortex. With the increase in fetal age i.e....

Research paper thumbnail of ASSESSMENT OF PHYTOCHEMICAL COMPOSITION AND ANTIBACTERIAL ACTIVITY OF DIFFERENT EXTRACTS OF Barleria prionitis LEAVES AGAINST ORAL MICROFLORA TO IMPROVE DENTAL HYGIENE

Oral diseases are major health problem with dental caries. Oral health influence the general qual... more Oral diseases are major health problem with dental caries. Oral health influence the general quality of life and poor oral health is linked to chronic conditions and systemic diseases. Hence there is an urgent need to find out an alternative way, instead of antibiotic and chemicals for the treatment of oral diseases which show more side effects. Present investigation is focused on antibacterial activity of aqueous, petroleum ether, chloroform and acetone extract of leaves of Barleria prionitis (Family-Acanthaceae) against seven bacterial species (Lactobacillus rhamnosus, Staphylococcus aureus , Bacillus subtilis , Actinomyces viscoscus, Staphylococcus epidermidis, Escherichia coli , Streptococcus mutans ) which are known to cause oral diseases in human being. Similarly leaves of Barleria prionitis were evaluated for its phytochemical composition. Antibacterial activity of the extracts was determined by paper disc diffusion method using 200mg/ml concentration of extract and there % z...

Research paper thumbnail of Histological and Histochemical Study on the Stomach and Intestine of Catfish Heteropneustes Fossilis (Bloch 1794)

The present work describes the histological and histochemical studies of stomach and intestine of... more The present work describes the histological and histochemical studies of stomach and intestine of Heteropneustes fossilis. The stomach and intestinal morphology is influenced by its feeding habits. The histological studies of stomach and intestine of H. fossilis revealed the basic pattern of wall layers as that other teleost fishes and vertebrates. However, few variations were observed in the thickness of muscle layers in the pyloric regions as compared to cardio-fundic region, which may be due to the variation in feeding habits. The stomach of H. fossilis consists of two parts cardio-fundic and pyloric. Mucosa layer of cardio-fundic region formed by numerous tapered fold covered by the columnar epithelium. The gastric glands are more in cardio-fundic region while rare in the pyloric region. Histochemically all the mucous secreting cells of the stomach are positive to the PAS stain and negative to the AB stain. Intestine of H. fossilis is long, coiled and divided into three parts; p...

Research paper thumbnail of A Preliminary checklist of Moths species from Amravati city, Maharashtra (India)

Present study was an attempt to explore moth diversity from different parts of the Amravati city.... more Present study was an attempt to explore moth diversity from different parts of the Amravati city. A total number of 628 moths were identified up to the family level. Families Sphingidae, Noctuidae, Geometridae, Crambidae, Arctiidae, Lymantiidae and Saturnidae were represented in the identified samples. The diversity index of Noctuidae family 2.63 was high as compared to the other families. While the lowest diversity index was found in family Geometridae and Saturnidae.

Research paper thumbnail of Asian Journal Of Biology and Biotechnology 2014 Histology, histochemical and ultrastructural studies on Intestine of Indian catfish, Clarias batrachus (Linn 1758)

Research paper thumbnail of Bioremediation of industrial waste by using bat guano

Journal of environmental science & engineering, 2007

The present investigation is an attempt to study the effect of bat guano with its rich microbial ... more The present investigation is an attempt to study the effect of bat guano with its rich microbial flora on bioremediation of industrial waste effluents. The results revealed that within a period of 15 days, there was a remarkable reduction in the Chemical Oxygen Demand (COD) values up to 50%-70%, thus stabilizing the industrial effluents. In addition to this,values of various physico-chemical parameters were notably found to reduce suggesting that industrial effluents can be effectively treated by bat guano.

Research paper thumbnail of A Preliminary Report on the Moths (Insecta: Lepidoptera: Heterocera) Fauna from Amravati, Maharashtra

Moths belong to the order Lepidoptera and this type of fauna are easily affected by a slightest c... more Moths belong to the order Lepidoptera and this type of fauna are easily affected by a slightest change in environment so keeping in view moths could be used to check the minute change of environment hence can be called as bioindicators of environment. Taking a chance to investigate environmental health all possible efforts were carried out in this work to list and unfold this hidden fauna of city and some surrounding areas. Collection of moths was carried out from August 2012 to January 2013 to determine their diversity and occurance. A total number of 41 moth specimens were collected by using simple light traps operated from dusk to dawn. The moths were identified up to family level. Families Noctuidae, Arctiidae, Geometridae, Sphingdae, Saturniidae, Crambidae, Lasiocampidae and Lymantridae were presented in collection samples. Keyword: Abundance, collection, diversity, identification, moth taxonomy.