li guang | Guangxi University of Technology (original) (raw)

Papers by li guang

Journal of Nuclear Cardiology, 2001

Background Iodine 123-labeled 15-(p-iodophenyl)-3-(R,S)-methylpentadecanoic acid (BMIPP) is mainl... more Background Iodine 123-labeled 15-(p-iodophenyl)-3-(R,S)-methylpentadecanoic acid (BMIPP) is mainly trapped in the myocardium as triglyceride, depending on the adenosine triphosphate level. Ten percent to 20% of it is metabolized through α-oxidation after β-oxidation; however, the precise mechanism of the regulatory pathways of BMIPP is yet to be clarified. Methods and Results A brief left coronary artery occlusion (10-30 minutes) was performed in 28 male Wistar-Kyoto rats. Dual single photon emission computed tomography images of BMIPP and thallium 201 were obtained 3 days and 24 days after the operation. The activities of 3-hydroxyacyl-coenzyme A dehydrogenase (HAD), citrate synthase (CS), and a-glycerol-phosphate dehydrogenase (GPD) were then measured in both ischemic and nonischemic regions. BMIPP and Tl-201 chloride severity scores were also evaluated conventionally. CS and HAD levels were significantly lower in the ischemic region than in the nonischemic region in the chronic group (CS, 102.9 ± 28.1 vs 138.7 ± 33.7 μmol/g/min, respectively, P = .0051; HAD, 54.7 ± 20.1 vs 78.6 ± 18.7 μmol/g/min, respectively, P = .0031). There was no difference in GPD between the ischemic and nonischemic regions. The BMIPP severity score had closer inverse relations with HAD (acute, r = -0.82; chronic, r = -0.80) and CS (acute, r = -0.87; chronic, r = -0.81), but not with GPD, than did Tl-201 chloride severity score. Conclusions BMIPP imaging correlates well with the activities of HAD and CS, suggesting that a decrease in BMIPP uptake reflects deterioration of both fatty acid metabolism and citrate cycle and shows information other than regional myocardial perfusion.

Thoracic and Cardiovascular Surgeon, 1990

Structure, 2006

MAP kinases bind activating kinases, phosphatases, and substrates through docking interactions. H... more MAP kinases bind activating kinases, phosphatases, and substrates through docking interactions. Here, we report a 1.9 Å crystallographic analysis of inactive ERK2 bound to a “D motif” docking peptide (pepHePTP) derived from hematopoietic tyrosine phosphatase, a negative regulator of ERK2. In this complex, the complete D motif interaction defined by mutagenic analysis is observed, including extensive electrostatic interactions with the “CD” site of the kinase. Large conformational changes occur in the activation loop where the dual phosphorylation sites, which are buried in the inactive form of ERK2, become exposed to solvent in the complex. Similar conformational changes occur in a complex between ERK2 and a MEK2 (MAP/ERK kinase-2)-derived D motif peptide (pepMEK2). D motif peptides are known to bind homologous loci in the MAP kinases p38α and JNK1, also inducing conformational changes in these enzymes. However, the binding interactions and conformational changes are unique to each, thus contributing to specificity among MAP kinases.

Atmospheric Environment, 2002

During the austral summers of 1999 and 2000, we made measurements of methane and nitrous oxide fl... more During the austral summers of 1999 and 2000, we made measurements of methane and nitrous oxide fluxes from tundra soils in maritime Antarctica, using a static open chamber technique. N 2 O and CH 4 fluxes from the nonornithogenic moss soils averaged 0.8 and À8.7 mg m À2 h À1 , respectively, in the summer of 1999 and 1.4 and 26.7 mg m À2 h À1 , respectively, in the summer of 2000. N 2 O flux from the ornithogenic soils was approximately two orders of magnitude greater than that from moss soils and averaged 89.7 mg m À2 h À1 , and CH 4 flux was approximately one order of magnitude greater and averaged 227.9 mg m À2 h À1 , indicating that high organic carbon and nitrogen compound of penguin droppings deposition in the soils resulted in high CH 4 and N 2 O emissions as a result of biologic transformation. The relationships between the fluxes and environmental factors were discussed. r

Transplantation, 2006

CD4CD25 regulatory T (Treg) cells are essential for the induction and maintenance of immunologic ... more CD4CD25 regulatory T (Treg) cells are essential for the induction and maintenance of immunologic self-tolerance as well as transplant tolerance. The effects of cyclosporin A (CsA), a widely used immunosuppressive agent, on CD4CD25Treg cells in mice were investigated. Balb/c mice were injected with CsA or control solution for one month. The levels, phenotype, and function of CD4CD25Treg cells in these mice were then assayed. The percentages and total cell numbers of CD4CD25Treg cells in the peripheral blood and spleen were significantly reduced after the treatment with CsA. The total numbers of CD4CD25Treg cells in the thymus of CsA-treated mice were markedly reduced as compared to the control mice. However, the percentage of CD4CD25Treg cells in the thymus of CsA-treated mice was markedly enhanced. More CD4CD25Treg cells expressing high levels of CD44 and CD45RB, and less CD4CD25Treg cells expressing CD62L were observed in CsA-treated mice, compared with the control mice. CD4CD25Treg cells expressed slightly lower levels of Foxp3 in CsA-treated mice. Furthermore, CsA markedly impaired the immunosuppressive function of CD4CD25Treg cells. CsA significantly impaired the development and function of CD4CD25Treg cells. The present studies suggest that CsA may block the potential induction of immune tolerance and increase the susceptibility to develop autoimmune diseases while preventing graft rejection.

Journal of Neurochemistry, 2002

Abstract: The effect of nociceptin/orphanin FQ (N/OFQ), an endogenous ligand for the newly identi... more Abstract: The effect of nociceptin/orphanin FQ (N/OFQ), an endogenous ligand for the newly identified opioid receptor-like (ORL1) receptor, on mitogen-activated protein kinase (MAPK) was investigated in Chinese hamster ovary cells stably expressing ORL1 receptor. N/OFQ rapidly stimulated phosphorylation and activity of MAPK (p42 and p44 isoforms) in a concentration-dependent manner. The p42 isoform was preferentially activated by N/OFQ. Maximal activation (5.4 ± 1.2-fold of basal for p42 isoform) was achieved after a 1-min exposure of cells to 100 nM N/OFQ. The activation was blocked completely by pretreatment with pertussis toxin, but was not reversed by naloxone. U-73122, a phospholipase C-specific inhibitor, significantly inhibited phospholipase C activity, as well as MAPK activation stimulated by N/OFQ. Furthermore, N/OFQ-stimulated MAPK activation was suppressed by a protein kinase C-specific inhibitor, chelerythrine. The results demonstrate that N/OFQ can effectively stimulate MAPK by the activation of ORL1 receptor and pertussis toxin-sensitive G proteins, and that phospholipase C, as well as protein kinase C, is critically involved in these processes.

Journal of Materials Processing Technology, 2008

Adaptive response surface method Particle swarm optimization intelligent sampling Sheet metal for... more Adaptive response surface method Particle swarm optimization intelligent sampling Sheet metal forming Metamodel a b s t r a c t In this study, the previously developed adaptive response surface method (ARSM) is suggested for construction of metamodel for highly non-linear responses. In order to develop the accuracy and efficiency of metamodel, the particle swarm optimization intelligent sampling (PSOIS) scheme is developed. This kind of intelligent method can guarantee the sampling search in right direction and constraint the bounds of design variables in feasible region. For validation of developed method, the Rosenbrock function is successfully approximated by proposed method; corresponding metamodel appropriateness can be well predicted by analysis of variance (ANOVA). Metamodel by ARSM with PSOIS are employed for optimization of initial blank shape and blank hold force (BHF) in sheet forming process, with validations by finite element simulations using LSDYNA970 commercial code.

This paper explores some of the practical issues associated with the use of case-based reasoning ... more This paper explores some of the practical issues associated with the use of case-based reasoning (CBR) or estimation by analogy. We note that different research teams have reported widely differing results with this technology. Whilst we accept that underlying characteristics of the datasets being used play a major role we also argue that configuring a CBR system can also have

Environmental Science & Technology, 2005

Molecular and Cellular Biology, 2000