Destaw Engidaw Takele | Debre Markos University (original) (raw)

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Papers by Destaw Engidaw Takele

Research paper thumbnail of Chemical constituents, physicochemical properties and antibacterial activity of leaves essential oil of Ocimum urticifolium

Journal of Coastal Life Medicine, 2016

The journal implements double-blind peer review practiced by specially invited international edit... more The journal implements double-blind peer review practiced by specially invited international editorial board members.

Research paper thumbnail of Effect of Season on the Major Physico-Chemical Parameters of Honey Product

Food and Environment Safety, 2020

The study was conducted to determine the major physic-chemical parameters and to investigate the ... more The study was conducted to determine the major physic-chemical parameters and to investigate the effects of season on the quality of honey product collected from Dawuro zone, Southern nationalities of people region of Ethiopia. The contents of physic-chemical parameters within the three honey production seasons ranged as: moisture content, 15-18%; electrical conductivity, 0.27-0.31mS/cm; pH, 4.0 - 4.5; free acidity, 18.7 - 24.7meq/kg; lactone acidity, 5.7 - 8.0meq/kg; HMF content, 16.1- 20.3mg/kg; reducing sugars, 70 - 72g/100g and sucrose content from 2.4 - 2.8g/100g. This study showed that there were some significant variations for the contents of the different physicochemical parameters between the different honey production districts and seasons. It was confirmed that the results were below the maximum permissible limit to conform to international standards concerning the good quality of honey. Although, the honey collected in this area should be consumed as fresh to avoid furth...

Research paper thumbnail of Study on Lungworm and Associated Risk Factors of Small Ruminants in Hitosa Woreda, Ethiopia

IDOSI Publications, 2016

A cross-sectional study was carried out from November, 2015 to April, 2016 in selected villages o... more A cross-sectional study was carried out from November, 2015 to April, 2016 in selected villages of Hitosaworeda, Oromia region, Ethiopia to determine prevalence, risk factors and identifying species of lungworm in small ruminants. Coproscopical examination was conducted on 400 (322 sheep and 78 goats). Simple random sampling technique was followed to select sampling units. Fecal samples from sheep and goats of all age groups and both sexes were examined by modified Baermann technique for the extraction of L1 larvae. The overall prevalence of lungworm infection was 45% in coproscopic examination. The prevalence of lungworm at sex level was 35.9% and 49.3% in male and female, respectively. The prevalence at species level was 43.8% and 50.0% in ovine and caprine, respectively. There was no statistical significance difference in the prevalence of lungworm infection between species of animals during coprological examination (p>0.05). However, there was statistical significant difference in the prevalence of lungworm infection on body condition (p<0.05). Identified species of lungworm were Muellerius capillaris, Dictyocaulus filaria, Protostrongylus rufescens and mixed in rate of 20.8%, 10.5, 2.8, 11% in coproscopic examination respectively. Mulerius capilaris had the highest prevalence in the study area than other species of lungworms. Due to its impact on production, emphasis should be given for the control and prevention of lungworm infection in this area.

Research paper thumbnail of Detection of the Concentration of Some Metals in the Honey Product in Dawuro Zone, SNNPR, Ethiopia

Journal of Analytical & Bioanalytical Techniques, 2019

Naturally honey contains a variety of mineral elements which mostly indicates the botanical origi... more Naturally honey contains a variety of mineral elements which mostly indicates the botanical origin of honey and contamination by environmental sources. This study was done to determine the concentration of metals and to investigate the effects of season and location on concentration of metals in honey produced in Dawuro zone. The ash content of honey was determined by muffle furnace and the content of metals was determined by flame atomic absorption spectrometry (AAS). The mean values of ash and different metals within the three honey production seasons in Dawuro zone were ranged as: ash content, 0.10-0.14 g/100 g and content of metals (mg/kg):

Research paper thumbnail of Chemical constituents, physicochemical properties and antibacterial activity of leaves essential oil of Ocimum urticifolium

Journal of Coastal Life Medicine, 2016

The journal implements double-blind peer review practiced by specially invited international edit... more The journal implements double-blind peer review practiced by specially invited international editorial board members.

Research paper thumbnail of Phytochemical Analysis, Antibacterial and Antioxidant Activity of the Leave Extracts of Ruta Chalepensis

IISTE, 2019

Rue (Ruta chalepensis) commonly known as rue, is traditionally used in Ethiopia for the treatment... more Rue (Ruta chalepensis) commonly known as rue, is traditionally used in Ethiopia for the treatment of a variety of diseases. Therefore this study conducted to investigate phytochemical constituents, antibacterial and antioxidant activity of the leave extracts of Ruta chalepensis. The phytochemical screening tests were conducted as per the standard procedure to identify the classes of compounds present in the leave extract of Ruta chalepensis. In vitro antibacterial activities of crude extracts were evaluated via disc diffusion method while antioxidant activities of extracts were determined by DPPH assay, the phosphomolybdenum method and total flavonoid contents were determined as per of the standard procedures. Phytochemical screening of leave extracts of Ruta chalepensis revealed the presence of secondary metabolites. The methanol extract had the greatest DPPH scavenging (93.851±0.148%) and total antioxidant (1.63 ± 0.19 mg AAE/g of dried extract) activities. Ethyl acetate extract showed the growth suppression of Bacillus cereus and Escherichia coli and methanol extract showed growth suppression of Bacillus cereus and Staphylococcus aureus with minimum inhibition concentration 50mg/mL. The antibacterial activities of the extracts were compared with a commercially available antibiotic (chloramphenicol) and showed moderate antibacterial activities, with inhibition zones ranging between 18-28 mm. Therefore the solvent extracts of Ruta chalepensis revealed the presence of important secondary metabolites, antibacterial and antioxidant activities.

Research paper thumbnail of EFFECT OF SEASON ON THE MAJOR PHYSICO-CHEMICAL PARAMETERS OF HONEY PRODUCT

food and environment safety, 2020

The study was conducted to determine the major physic-chemical parameters and to investigate the ... more The study was conducted to determine the major physic-chemical parameters and to investigate the effects of season on the quality of honey product collected from Dawuro zone, Southern nationalities of people region of Ethiopia. The contents of physic-chemical parameters within the three honey production seasons ranged as: moisture content, 15-18%; electrical conductivity, 0.27-0.31mS/cm; pH, 4.0-4.5; free acidity, 18.7-24.7meq/kg; lactone acidity, 5.7-8.0meq/kg; HMF content, 16.1-20.3mg/kg; reducing sugars, 70-72g/100g and sucrose content from 2.4-2.8g/100g. This study showed that there were some significant variations for the contents of the different physicochemical parameters between the different honey production districts and seasons. It was confirmed that the results were below the maximum permissible limit to conform to international standards concerning the good quality of honey. Although, the honey collected in this area should be consumed as fresh to avoid further contamination. Moreover, further studies of other quality parameters of honey are needed.

Research paper thumbnail of Chemical constituents, physicochemical properties and antibacterial activity of leaves essential oil of Ocimum urticifolium

Journal of Coastal Life Medicine, 2016

The journal implements double-blind peer review practiced by specially invited international edit... more The journal implements double-blind peer review practiced by specially invited international editorial board members.

Research paper thumbnail of Effect of Season on the Major Physico-Chemical Parameters of Honey Product

Food and Environment Safety, 2020

The study was conducted to determine the major physic-chemical parameters and to investigate the ... more The study was conducted to determine the major physic-chemical parameters and to investigate the effects of season on the quality of honey product collected from Dawuro zone, Southern nationalities of people region of Ethiopia. The contents of physic-chemical parameters within the three honey production seasons ranged as: moisture content, 15-18%; electrical conductivity, 0.27-0.31mS/cm; pH, 4.0 - 4.5; free acidity, 18.7 - 24.7meq/kg; lactone acidity, 5.7 - 8.0meq/kg; HMF content, 16.1- 20.3mg/kg; reducing sugars, 70 - 72g/100g and sucrose content from 2.4 - 2.8g/100g. This study showed that there were some significant variations for the contents of the different physicochemical parameters between the different honey production districts and seasons. It was confirmed that the results were below the maximum permissible limit to conform to international standards concerning the good quality of honey. Although, the honey collected in this area should be consumed as fresh to avoid furth...

Research paper thumbnail of Study on Lungworm and Associated Risk Factors of Small Ruminants in Hitosa Woreda, Ethiopia

IDOSI Publications, 2016

A cross-sectional study was carried out from November, 2015 to April, 2016 in selected villages o... more A cross-sectional study was carried out from November, 2015 to April, 2016 in selected villages of Hitosaworeda, Oromia region, Ethiopia to determine prevalence, risk factors and identifying species of lungworm in small ruminants. Coproscopical examination was conducted on 400 (322 sheep and 78 goats). Simple random sampling technique was followed to select sampling units. Fecal samples from sheep and goats of all age groups and both sexes were examined by modified Baermann technique for the extraction of L1 larvae. The overall prevalence of lungworm infection was 45% in coproscopic examination. The prevalence of lungworm at sex level was 35.9% and 49.3% in male and female, respectively. The prevalence at species level was 43.8% and 50.0% in ovine and caprine, respectively. There was no statistical significance difference in the prevalence of lungworm infection between species of animals during coprological examination (p>0.05). However, there was statistical significant difference in the prevalence of lungworm infection on body condition (p<0.05). Identified species of lungworm were Muellerius capillaris, Dictyocaulus filaria, Protostrongylus rufescens and mixed in rate of 20.8%, 10.5, 2.8, 11% in coproscopic examination respectively. Mulerius capilaris had the highest prevalence in the study area than other species of lungworms. Due to its impact on production, emphasis should be given for the control and prevention of lungworm infection in this area.

Research paper thumbnail of Detection of the Concentration of Some Metals in the Honey Product in Dawuro Zone, SNNPR, Ethiopia

Journal of Analytical & Bioanalytical Techniques, 2019

Naturally honey contains a variety of mineral elements which mostly indicates the botanical origi... more Naturally honey contains a variety of mineral elements which mostly indicates the botanical origin of honey and contamination by environmental sources. This study was done to determine the concentration of metals and to investigate the effects of season and location on concentration of metals in honey produced in Dawuro zone. The ash content of honey was determined by muffle furnace and the content of metals was determined by flame atomic absorption spectrometry (AAS). The mean values of ash and different metals within the three honey production seasons in Dawuro zone were ranged as: ash content, 0.10-0.14 g/100 g and content of metals (mg/kg):

Research paper thumbnail of Chemical constituents, physicochemical properties and antibacterial activity of leaves essential oil of Ocimum urticifolium

Journal of Coastal Life Medicine, 2016

The journal implements double-blind peer review practiced by specially invited international edit... more The journal implements double-blind peer review practiced by specially invited international editorial board members.

Research paper thumbnail of Phytochemical Analysis, Antibacterial and Antioxidant Activity of the Leave Extracts of Ruta Chalepensis

IISTE, 2019

Rue (Ruta chalepensis) commonly known as rue, is traditionally used in Ethiopia for the treatment... more Rue (Ruta chalepensis) commonly known as rue, is traditionally used in Ethiopia for the treatment of a variety of diseases. Therefore this study conducted to investigate phytochemical constituents, antibacterial and antioxidant activity of the leave extracts of Ruta chalepensis. The phytochemical screening tests were conducted as per the standard procedure to identify the classes of compounds present in the leave extract of Ruta chalepensis. In vitro antibacterial activities of crude extracts were evaluated via disc diffusion method while antioxidant activities of extracts were determined by DPPH assay, the phosphomolybdenum method and total flavonoid contents were determined as per of the standard procedures. Phytochemical screening of leave extracts of Ruta chalepensis revealed the presence of secondary metabolites. The methanol extract had the greatest DPPH scavenging (93.851±0.148%) and total antioxidant (1.63 ± 0.19 mg AAE/g of dried extract) activities. Ethyl acetate extract showed the growth suppression of Bacillus cereus and Escherichia coli and methanol extract showed growth suppression of Bacillus cereus and Staphylococcus aureus with minimum inhibition concentration 50mg/mL. The antibacterial activities of the extracts were compared with a commercially available antibiotic (chloramphenicol) and showed moderate antibacterial activities, with inhibition zones ranging between 18-28 mm. Therefore the solvent extracts of Ruta chalepensis revealed the presence of important secondary metabolites, antibacterial and antioxidant activities.

Research paper thumbnail of EFFECT OF SEASON ON THE MAJOR PHYSICO-CHEMICAL PARAMETERS OF HONEY PRODUCT

food and environment safety, 2020

The study was conducted to determine the major physic-chemical parameters and to investigate the ... more The study was conducted to determine the major physic-chemical parameters and to investigate the effects of season on the quality of honey product collected from Dawuro zone, Southern nationalities of people region of Ethiopia. The contents of physic-chemical parameters within the three honey production seasons ranged as: moisture content, 15-18%; electrical conductivity, 0.27-0.31mS/cm; pH, 4.0-4.5; free acidity, 18.7-24.7meq/kg; lactone acidity, 5.7-8.0meq/kg; HMF content, 16.1-20.3mg/kg; reducing sugars, 70-72g/100g and sucrose content from 2.4-2.8g/100g. This study showed that there were some significant variations for the contents of the different physicochemical parameters between the different honey production districts and seasons. It was confirmed that the results were below the maximum permissible limit to conform to international standards concerning the good quality of honey. Although, the honey collected in this area should be consumed as fresh to avoid further contamination. Moreover, further studies of other quality parameters of honey are needed.