Anke Twigg-Flesner | Hartpury College (original) (raw)

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Papers by Anke Twigg-Flesner

Research paper thumbnail of The effects of two endocrine treatments on the size of ovulatory follicles in Lusitano mares in Portugal.

Research paper thumbnail of The effects of two endocrine treatments on the size of ovulatory follicles in Lusitano mares in Portugal

Research paper thumbnail of Investigating follicle growth, uterine oedema and other factors affecting reproductive success in the Lusitano mare

A total of n=159 Lusitano mares and n=309 reproductive cycles were analysed to model follicle siz... more A total of n=159 Lusitano mares and n=309 reproductive cycles were analysed to model follicle sizes at the time of ovulation and reproductive success in dependency of several variables. Uterine oedema was found to correlate positively with probability of successful pregnancy (p<0.05). Follicle sizes at the time of ovulation are affected by treatment with hCG (p<0.05), with lower sizes found for treated mares. Follicle size at the time of ovulation correlates negatively with the mares’ age (p<0.05) (Pearson’s r = -0.168). This has implications for the way we monitor follicular growth and intervene in breeding, specific to the Lusitano breed, to accurately time artificial insemination.

Research paper thumbnail of The effects of equine semen storage on the success rate of embryo recovery in a commercial setting

Research paper thumbnail of Lusitano mare reproduction: contribution to the knowledge

Research paper thumbnail of The effect of four commercially available extenders on motility of chilled stallion semen over time

Research paper thumbnail of New insights into the phylogeny of Entamoeba species provided by analysis of four new small-subunit rRNA genes

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2006

Sequences of small-subunit rRNA genes have been obtained for four new isolates of Entamoeba. Phyl... more Sequences of small-subunit rRNA genes have been obtained for four new isolates of Entamoeba. Phylogenetic analyses give new insights into the evolution of these organisms. A novel Entamoeba from pigs in Vietnam that produces uninucleate cysts proved to be unrelated to other uninucleated cyst-producing species. Revival of the name Entamoeba suis for this organism is proposed. Instead of being related to Entamoeba polecki, it shares a recent common ancestor with the non-encysting Entamoeba gingivalis in a lineage that is basal to the tetranucleate cystproducing clade. This suggests that species producing cysts with four nuclei are descended from an ancestor that produced cysts with a single nucleus. An Entamoeba from a horse was isolated in culture. No cysts were observed in the original stool sample but the sequence is placed unequivocally within the clade of tetranucleate cyst-producing species with no other sequences being specifically related. Revival of the name Entamoeba equi for this organism is proposed. The Entamoeba ecuadoriensis sequence was found to be the most closely related to Entamoeba histolytica and Entamoeba dispar, as predicted, despite the organism having been an environmental isolate originally assigned to Entamoeba moshkovskii. Finally, a partial E. polecki gene sequence from a pig proved to be virtually identical to that of Entamoeba struthionis from an ostrich, suggesting that the latter name is a synonym.

Research paper thumbnail of The effects of two endocrine treatments on the size of ovulatory follicles in Lusitano mares in Portugal.

Research paper thumbnail of The effects of two endocrine treatments on the size of ovulatory follicles in Lusitano mares in Portugal

Research paper thumbnail of Investigating follicle growth, uterine oedema and other factors affecting reproductive success in the Lusitano mare

A total of n=159 Lusitano mares and n=309 reproductive cycles were analysed to model follicle siz... more A total of n=159 Lusitano mares and n=309 reproductive cycles were analysed to model follicle sizes at the time of ovulation and reproductive success in dependency of several variables. Uterine oedema was found to correlate positively with probability of successful pregnancy (p<0.05). Follicle sizes at the time of ovulation are affected by treatment with hCG (p<0.05), with lower sizes found for treated mares. Follicle size at the time of ovulation correlates negatively with the mares’ age (p<0.05) (Pearson’s r = -0.168). This has implications for the way we monitor follicular growth and intervene in breeding, specific to the Lusitano breed, to accurately time artificial insemination.

Research paper thumbnail of The effects of equine semen storage on the success rate of embryo recovery in a commercial setting

Research paper thumbnail of Lusitano mare reproduction: contribution to the knowledge

Research paper thumbnail of The effect of four commercially available extenders on motility of chilled stallion semen over time

Research paper thumbnail of New insights into the phylogeny of Entamoeba species provided by analysis of four new small-subunit rRNA genes

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2006

Sequences of small-subunit rRNA genes have been obtained for four new isolates of Entamoeba. Phyl... more Sequences of small-subunit rRNA genes have been obtained for four new isolates of Entamoeba. Phylogenetic analyses give new insights into the evolution of these organisms. A novel Entamoeba from pigs in Vietnam that produces uninucleate cysts proved to be unrelated to other uninucleated cyst-producing species. Revival of the name Entamoeba suis for this organism is proposed. Instead of being related to Entamoeba polecki, it shares a recent common ancestor with the non-encysting Entamoeba gingivalis in a lineage that is basal to the tetranucleate cystproducing clade. This suggests that species producing cysts with four nuclei are descended from an ancestor that produced cysts with a single nucleus. An Entamoeba from a horse was isolated in culture. No cysts were observed in the original stool sample but the sequence is placed unequivocally within the clade of tetranucleate cyst-producing species with no other sequences being specifically related. Revival of the name Entamoeba equi for this organism is proposed. The Entamoeba ecuadoriensis sequence was found to be the most closely related to Entamoeba histolytica and Entamoeba dispar, as predicted, despite the organism having been an environmental isolate originally assigned to Entamoeba moshkovskii. Finally, a partial E. polecki gene sequence from a pig proved to be virtually identical to that of Entamoeba struthionis from an ostrich, suggesting that the latter name is a synonym.

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