Victor Arias | Fundação Antônio Prudente (original) (raw)
Papers by Victor Arias
Jornal Brasileiro de Patologia e …, 2003
... Victor Arias I ; Pedro Luiz Mazza II ; Marcelo Antonio Aranha Funke II. ... capazes de reconh... more ... Victor Arias I ; Pedro Luiz Mazza II ; Marcelo Antonio Aranha Funke II. ... capazes de reconhecer epitopos que não se degradam durante o processamento histológico, tem, na prática, gradativamente, substituído o método bioquímico, antes tido como o padrão-ouro, para a ...
Reproductive Sciences, 2015
The aim of this study was to analyze cell kinetics through expression and apoptosis of topoisomer... more The aim of this study was to analyze cell kinetics through expression and apoptosis of topoisomerase 2-α (TOP2A), p53, and c-erb2 in rectosigmoid endometriotic lesions and in healthy endometrial tissue and to establish correlations between such findings and clinical data in patients with rectosigmoid endometriosis. Sixty patients with rectosigmoid endometriosis and 20 control women without endometriosis were included. Immunohistochemical assays were used to measure expression of TOP2A, p53, and c-erB-2. Apoptosis was quantified by directly counting the apoptotic bodies. The number of lesions was positively correlated with expression of TOP2A in the lesion. There was also significant correlation between the lesions' size and number and cell turnover index. Apoptosis index (AI) was the same for endometriosis lesions and eutopic endometrium. Expression of TOP2A was significantly lower in the endometriosis group compared to the controls. Changes in cell proliferation but not in the AI in rectosigmoid endometriosis are indicative of an imbalance in cell kinetics that may lead to the development of the disease.
Analytica Chimica Acta, 1995
The fat-soluble vitamins retinyl palmitate, D3, E and K1 were separated on a packed reversed-phas... more The fat-soluble vitamins retinyl palmitate, D3, E and K1 were separated on a packed reversed-phase fused-silica capillary column using methanol-tetrahydrofuran as mobile phase, and determined with UV detection. Conventional liquid Chromatographic equipment was adapted for such purposes, providing a highly efficient and sensitive analytical system. The vitamins could be separated isocratically with detection limits of 2.9 pg, 1 pg, 29
Leukemia & Lymphoma, 2000
Tumor angiogenesis is required for tumor growth and metastasis. A statistically significant corre... more Tumor angiogenesis is required for tumor growth and metastasis. A statistically significant correlation has been demonstrated between prognosis and the microvessel density (a measure of tumor angiogenesis) of solid tumors, particularly of the breast and prostate, and lymphoid neoplasms. The aim of this study was to establish whether a correlation exists between vascular density and the malignant category of Non-Hodgkin's Lymphoma (NHL) defined by two classification systems (Kiel and Working Formulation). We also tested whether florid follicular hyperplasia (FFH) and follicular lymphomas (FL) behave as new vessel stimulating conditions. Eighty-nine NHL lymph node biopsies were reviewed and categorized according to the Kiel Classification and Working Formulation. Twelve FL were also selected and compared to 12 FFH biopsies. Vessels were highlighted by immunostaining with anti-Factor VIII antibody and quantified both by counting higher vascular density fields and by estimating the proportional vascular area. The results showed a statistically significant difference between low and high grade NHL, when classified in either the Working Formulation (p=0.0015) or the Kiel Classification (p=0.002). No differences were found in vessel counts between Working formulation intermediate and high grade lymphomas. Vascular density is similar when FFH and FL interfollicular areas are compared.
Revista do Colégio Brasileiro de Cirurgiões, 2004
... Adedamola Ladipo1; Marcos Brasilino de Carvalho2; Abrão Rapoport, ECBC-SP2; Victor Eduardo Ar... more ... Adedamola Ladipo1; Marcos Brasilino de Carvalho2; Abrão Rapoport, ECBC-SP2; Victor Eduardo Arrua Arias3; Luiz Carlos Filgueiras Leiro1 ... Estudo anatomopatológico de timos de pa-cientes miastênicos, confirmou as alterações patológicas e verificou que 70% dos timos ...
Revista da Associação Médica Brasileira, 2004
OBJETIVO: O retinoblastoma é o tumor intra-ocular mais freqüente na infância. A forma de apresent... more OBJETIVO: O retinoblastoma é o tumor intra-ocular mais freqüente na infância. A forma de apresentação mais comum é leucocoria, seguida pelo estrabismo. Nos países em desenvolvimento, pela demora diagnóstica e maior tempo de encaminhamento, encontramos um grande número de pacientes portadores de tumores extra-oculares. MÉTODOS: Duzentos e trinta e nove pacientes com retinoblastoma admitidos nos departamentos de Pediatria e Oftalmologia do Hospital do Câncer AC Camargo, no período de 1986 a 1995, foram avaliados quanto ao tempo de encaminhamento e extensão do tumor. A análise estatística incluiu o cálculo de freqüências absolutas e relativas, bem como o teste exato de Fisher, para a verificação da associação entre variáveis categorias. RESULTADOS: Pacientes que foram encaminhados em um período menor que 6 meses apresentaram maior freqüência de doença intra-ocular quando comparados com pacientes com tempo de encaminhamento maior que seis meses (75% vs. 25% p<0,001). Não houve diferença estatisticamente significativa para tumores extra-oculares. CONCLUSÕES: O diagnóstico precoce é considerado o ponto mais importante no tratamento de pacientes com retinoblastoma. Os pediatras são capazes de detectar, em uma consulta simples de ambulatório, os sinais e sintomas desta doença e devem ter sempre em mente o diagnóstico de retinoblastoma, possibilitando o encaminhamento precoce a centros especializados.
Ultrasound in Medicine & Biology, 2010
This was a study on the effects of 3-MHz ultrasound at 16-and 100-Hz pulse repetition frequencies... more This was a study on the effects of 3-MHz ultrasound at 16-and 100-Hz pulse repetition frequencies on angiogenesis and viability of random-pattern skin flaps in rats. A cranially-based dorsal skin flap was raised in 60 EPM-Wistar rats, which were randomly divided into four groups: control, sham, 16-Hz and 100-Hz groups. The mean percentage of necrosis was as follows: control, 42% ± 13%; sham, 18% ± 13%; 16-Hz group, 13% ± 10%; and 100-Hz group, 15% ± 7%, with significant differences between the control and the other groups (p , 0.001). The mean vascular density was as follows: control, 5% ± 2%; sham, 7% ± 2%; 16-Hz group, 21% ± 4%; and 100-Hz group, 24% ± 10%, with significant differences between control and ultrasound groups, and between the sham and ultrasound groups (p , 0.001). Both ultrasound treatments (16-and 100-Hz PRFs) induced angiogenesis, and sham and ultrasound treatments improved viability of random-pattern skin flaps in rats.
Plastic and Reconstructive Surgery, 2010
The transverse rectus abdominis musculocutaneous (TRAM) flap may develop necrosis, especially in ... more The transverse rectus abdominis musculocutaneous (TRAM) flap may develop necrosis, especially in patients with risk factors such as previous abdominoplasty, caused by damage to perforating vessels during surgical procedures. This study was designed from the perspective of using vascular endothelial growth factor (VEGF) gene therapy with plasmid vector after abdominoplasty to stimulate neovascularization of the TRAM flap, thus increasing flap viability. Thirty-two Wistar rats were divided into four groups (n = 8). A right inferiorly based TRAM flap was constructed in all animals and was the only procedure performed in group I (TRAM flap). Animals from groups II (abdominoplasty) and III (plasmid) underwent abdominoplasty and were injected intramuscularly with physiologic saline solution and empty plasmid, respectively. Group IV (VEGF) received intramuscular injection of naked plasmid DNA encoding VEGF-165 during abdominoplasty. The TRAM flap was created 30 days after abdominoplasty. The mean necrosis was 24.65 +/- 18.13 percent in group I, 62.49 +/- 28.06 percent in group II, 57.80 +/- 25.43 percent in group III, and 18.33 +/- 16.20 percent in group IV. The number of vessels in the TRAM flap was determined by immunohistochemistry using the antibody human heart factor. Groups I and IV had a similar number of vessels, as did groups II and III. Groups I and IV had greater viability and number of vessels than groups II and III. VEGF gene therapy increased viability and vessel number in the TRAM flap created after abdominoplasty in a rat model.
Photomedicine and Laser Surgery, 2010
To assess the effects of low-level laser therapy (LLLT) with output power of 30 and 60 mW in the ... more To assess the effects of low-level laser therapy (LLLT) with output power of 30 and 60 mW in the viability of a random skin flap in rats. Output power values in LLLT are not well defined. Controlled, single-blind experimental study. Thirty-six animals were randomly distributed into three groups: sham group (SG), 30-mW output power (30G), and 60-mW output power (60G). In both treated groups, a fluency of 3 J/cm(2) was used. Two minutes after elevation of a random-pattern cranially based dorsal flap (4 x 10 cm), laser irradiation was applied and repeated on the first, second, third, and fourth postoperative days. Percentages of flap necrosis were calculated on the seventh postoperative day. Also, four fragments of each flap were collected to allow determination of the percentages of vascular density according to the bidimensional method of the morphometric analysis of blood vessels. Statistical analysis included the Wilcoxon test and Kruskal-Wallis variance analysis. A significance level of 5% was elected (p < 0.05). Laser-treated animals presented significantly less necrosis than the sham group (SG, 53%; 30G, 24%; p < 0.05) (60G, 25%; p < 0.05). Also, laser-treated animals presented significantly more vascular density than the sham group (SG, 37%; 30G, 57%; p < 0.05) (60G, 59%; p < 0.05). LLLT (660 nm) with 30-mW and 60-mW output power was efficient in the increase of skin flap viability, but there was no difference between them.
Pediatric Blood & Cancer, 2007
To assess the outcome and determine risk factors for extraocular relapse in patients with retinob... more To assess the outcome and determine risk factors for extraocular relapse in patients with retinoblastoma who had been enucleated after failure of chemoreduction. Retrospective study (1995-2002) at three institutions. Pathological risk factors (PRF) were defined as invasion of the anterior segment, choroid, post-laminar optic nerve, subarachnoid space, or sclera according to the local pathology report. Extraocular relapse was defined as an event. One hundred twenty-two patients were included (17 had bilateral enucleation). Chemoreduction included vincristine, carboplatin, and etoposide (n=80, 65.6%), vincristine, and carboplatin (n=17, 13.9%), or carboplatin (n=25, 20.5%). Thirty-five also received external beam radiotherapy (28.7%). PRF included: 39 with choroidal involvement, 9 with anterior segment, 9 with scleral, and 2 with post-laminar optic nerve with subarachnoid invasion. Adjuvant chemotherapy was given to eight patients (6.5%) because of scleral invasion. Four patients had an extraocular relapse after enucleation, two of whom survive after intensive treatment including stem cell rescue. Five-year probability of event-free survival is 0.96. Only scleral invasion and bilateral enucleation were significantly associated with extraocular relapse. The risk of extraocular relapse is low after enucleation following failure of chemoreduction. Patients who underwent bilateral enucleation and those with scleral invasion are at higher risk of extraocular relapse.
Pediatric Blood & Cancer, 2007
Retinoblastoma (RB) is the most common intraocular malignancy found in children. It is caused by ... more Retinoblastoma (RB) is the most common intraocular malignancy found in children. It is caused by the inactivation of both copies of a tumor suppressor gene (Rb1), which participates in the control of cell cycling (1-3). Approximately one-third of these tumors are bilateral and is associated with germinal mutations. All bilateral tumors and one tenth of unilateral tumors are caused by a germline mutation inherited as an autosomal dominant trait (4, 5). This hereditary predisposition to retinoblastoma is caused by mutant alleles occurring at the q14 band of chromosome 13 (6). The association of bilateral RB with ectopic midline intracranial tumors, termed "trilateral"retinoblastoma (TRB), is a well recognized but uncommon syndrome. The association of ocular RB with brain tumors was first reported in 1971 (7). The intracranial tumor arises most often in the pineal region but can also be a suprasellar or parasellar tumor, and is considered to be an isolated independent primary focus without evidence of retinal disease. This is the first reported case of TRB presented with a sellar and suprasellar tumor in Korea.
Pediatric Blood & Cancer, 2007
Retinoblastoma is a malignant tumor of the embryonic neural retina. About 80% of cases are diagno... more Retinoblastoma is a malignant tumor of the embryonic neural retina. About 80% of cases are diagnosed before age 4, with a median age at diagnosis of 2 years. To determine characteristics and prognosis of retinoblastoma in children older than 5 years. From 1986 to 2002, medical records of 16 patients out of 453 cases referred to Hospital do Câncer AC Camargo, São Paulo, Brazil. Median age at diagnosis was 73.7 months (range 65-144) and there was an equal gender distribution. Fifteen patients presented with unilateral disease. The mean time between first symptoms and diagnosis was 9.6 months (range 0-48). Most cases were diagnosed in advanced stages and 15 eyes were enucleated. Eleven patients presented with intraocular tumor (1 Reese II and 10 Reese V) and five presented with extraocular disease (one CCG II and four CCG III). Twelve patients are still alive with a median follow-up of 92 months (range 65-199). Because of its low incidence at this age, diagnosis of retinoblastoma is usually delayed due to low level of suspicion. Therefore, it is important that physicians are aware of this disease in order to perform an earlier diagnosis, and decrease treatment-related morbidity.
Medical and Pediatric Oncology, 1999
Rev Assoc Med Bras 2004; 50(4): 400-2 400 ANTONELI CBG ET AL. RESUMO -OBJETIVO. O retinoblastoma ... more Rev Assoc Med Bras 2004; 50(4): 400-2 400 ANTONELI CBG ET AL. RESUMO -OBJETIVO. O retinoblastoma é o tumor intra-ocular mais freqüente na infância. A forma de apresentação mais comum é leucocoria, seguida pelo estrabismo. Nos países em desenvolvimento, pela demora diagnóstica e maior tempo de encaminhamento, encontramos um grande número de pacientes portadores de tumores extra-oculares. MÉTODOS. Duzentos e trinta e nove pacientes com retinoblastoma admitidos nos departamentos de Pediatria e Oftalmologia do Hospital do Câncer AC Camargo, no período de 1986 a 1995, foram avaliados quanto ao tempo de encaminhamento e extensão do tumor. A análise estatística incluiu o cálculo de freqüências absolutas e relativas, bem como o teste exato de Fisher, para a verificação da associação entre variáveis categorias. RESULTADOS. Pacientes que foram encaminhados em um
Revista Da Associacao Medica Brasileira, 2004
OLIVEIRA AB ET AL.
Leukemia and Lymphoma, 2000
Tumor angiogenesis is required for tumor growth and metastasis. A statistically significant corre... more Tumor angiogenesis is required for tumor growth and metastasis. A statistically significant correlation has been demonstrated between prognosis and the microvessel density (a measure of tumor angiogenesis) of solid tumors, particularly of the breast and prostate, and lymphoid neoplasms. The aim of this study was to establish whether a correlation exists between vascular density and the malignant category of Non-Hodgkin's Lymphoma (NHL) defined by two classification systems (Kiel and Working Formulation). We also tested whether florid follicular hyperplasia (FFH) and follicular lymphomas (FL) behave as new vessel stimulating conditions. Eighty-nine NHL lymph node biopsies were reviewed and categorized according to the Kiel Classification and Working Formulation. Twelve FL were also selected and compared to 12 FFH biopsies. Vessels were highlighted by immunostaining with anti-Factor VIII antibody and quantified both by counting higher vascular density fields and by estimating the proportional vascular area. The results showed a statistically significant difference between low and high grade NHL, when classified in either the Working Formulation (p=0.0015) or the Kiel Classification (p=0.002). No differences were found in vessel counts between Working formulation intermediate and high grade lymphomas. Vascular density is similar when FFH and FL interfollicular areas are compared.
Journal of Plastic, Reconstructive & Aesthetic Surgery, 2010
Background: The angiogenic induction property of the omentum makes it a promising pedicle for pre... more Background: The angiogenic induction property of the omentum makes it a promising pedicle for prefabricating flaps. Therefore, the objective of this paper is to establish the abdominal area to be prefabricated by the omental pedicle and to analyse the prefabricated potential (PP) according to the time delay between the pedicle introduction and the flap release. Methods: Forty-four rabbits were divided into four groups (A, B, C and D). In group A, a piece of skin, subcutaneous tissue and abdominal cutaneous muscle was fully released and sutured again in place. In the other groups, a 9-cm 2 omental pedicle containing the gastromental vessels distally tied was transposed and sutured to abdominal cutaneous muscle. A second procedure, consisting of incision and release of the flap that contained skin, subcutaneous and cutaneous abdominal muscle pediculated only by the omentum, was carried out. The only variation was the time delay between the two procedures: 7, 21 and 56 days for groups B, C and D, respectively. The flaps were inspected 15 days after the last procedure. The pieces of viable area were immunostained using anti-CD31 for estimation of the microvascular density. Results: The mean and maximum viable areas in group D were 45.29 and 99.37 cm 2 , respectively (average PP Z 5.03 and maximum PP Z 11.04). There was no significant difference between the viable areas in groups C and D. The mean microvascular densities of groups B, C and D were 24.54, 33.20 and 27.03 vessels/mm 2 , respectively.
Journal of Pediatric Hematology/Oncology, 2007
This study aimed to determine the impact of the addition of ifosfamide/etoposide to a regimen con... more This study aimed to determine the impact of the addition of ifosfamide/etoposide to a regimen containing cisplatin/teniposide on the survival of patients with retinoblastoma with orbital involvement. Thirty patients were treated at the A. C. Camargo Hospital, Brazil, from 1986 to 2002. From 1986 to April 1992 (period I, n=12), treatment consisted of 3 cycles of induction chemotherapy with cisplatin and teniposide, followed by maintenance with same drugs alternating with cyclophosphamide, vincristine, and doxorubicin every 21 days for 60 weeks. Since April 1992 (period II, n=18), the treatment consisted of 3 cycles of ifosfamide and etoposide followed by maintenance with same drugs, alternating with cisplatin and teniposide every 21 days for 36 weeks. In both periods, children were submitted to exenteration with eyelid preservation and orbital radiation therapy with 45 cGy, and also received intrathecal therapy with methotrexate plus dexamethasone and cytarabine. Kaplan-Meier method was used for survival analysis. The median age was 31 months. Most patients (86.7%) presented unilateral tumors. The 3-year overall survival was 34.4% and 72.2%, respectively, for patients treated during periods I and II (P=0.061). The addition of ifosfamide/etoposide to chemotherapy with cisplatin/teniposide improves survival in these patients, but further studies are still necessary.
Clinical Nutrition, 2008
Conclusions: LE, independently of composition, had no influence on neutrophils' chemotaxis, but s... more Conclusions: LE, independently of composition, had no influence on neutrophils' chemotaxis, but showed different effect on phagocytosis by macrophages. SMOF LE had neutral effect while fish oil LE enriched with MCT/LCT LE increased resident-macrophages' phagocytosis. ª
Jornal Brasileiro de Patologia e …, 2003
... Victor Arias I ; Pedro Luiz Mazza II ; Marcelo Antonio Aranha Funke II. ... capazes de reconh... more ... Victor Arias I ; Pedro Luiz Mazza II ; Marcelo Antonio Aranha Funke II. ... capazes de reconhecer epitopos que não se degradam durante o processamento histológico, tem, na prática, gradativamente, substituído o método bioquímico, antes tido como o padrão-ouro, para a ...
Reproductive Sciences, 2015
The aim of this study was to analyze cell kinetics through expression and apoptosis of topoisomer... more The aim of this study was to analyze cell kinetics through expression and apoptosis of topoisomerase 2-α (TOP2A), p53, and c-erb2 in rectosigmoid endometriotic lesions and in healthy endometrial tissue and to establish correlations between such findings and clinical data in patients with rectosigmoid endometriosis. Sixty patients with rectosigmoid endometriosis and 20 control women without endometriosis were included. Immunohistochemical assays were used to measure expression of TOP2A, p53, and c-erB-2. Apoptosis was quantified by directly counting the apoptotic bodies. The number of lesions was positively correlated with expression of TOP2A in the lesion. There was also significant correlation between the lesions' size and number and cell turnover index. Apoptosis index (AI) was the same for endometriosis lesions and eutopic endometrium. Expression of TOP2A was significantly lower in the endometriosis group compared to the controls. Changes in cell proliferation but not in the AI in rectosigmoid endometriosis are indicative of an imbalance in cell kinetics that may lead to the development of the disease.
Analytica Chimica Acta, 1995
The fat-soluble vitamins retinyl palmitate, D3, E and K1 were separated on a packed reversed-phas... more The fat-soluble vitamins retinyl palmitate, D3, E and K1 were separated on a packed reversed-phase fused-silica capillary column using methanol-tetrahydrofuran as mobile phase, and determined with UV detection. Conventional liquid Chromatographic equipment was adapted for such purposes, providing a highly efficient and sensitive analytical system. The vitamins could be separated isocratically with detection limits of 2.9 pg, 1 pg, 29
Leukemia & Lymphoma, 2000
Tumor angiogenesis is required for tumor growth and metastasis. A statistically significant corre... more Tumor angiogenesis is required for tumor growth and metastasis. A statistically significant correlation has been demonstrated between prognosis and the microvessel density (a measure of tumor angiogenesis) of solid tumors, particularly of the breast and prostate, and lymphoid neoplasms. The aim of this study was to establish whether a correlation exists between vascular density and the malignant category of Non-Hodgkin's Lymphoma (NHL) defined by two classification systems (Kiel and Working Formulation). We also tested whether florid follicular hyperplasia (FFH) and follicular lymphomas (FL) behave as new vessel stimulating conditions. Eighty-nine NHL lymph node biopsies were reviewed and categorized according to the Kiel Classification and Working Formulation. Twelve FL were also selected and compared to 12 FFH biopsies. Vessels were highlighted by immunostaining with anti-Factor VIII antibody and quantified both by counting higher vascular density fields and by estimating the proportional vascular area. The results showed a statistically significant difference between low and high grade NHL, when classified in either the Working Formulation (p=0.0015) or the Kiel Classification (p=0.002). No differences were found in vessel counts between Working formulation intermediate and high grade lymphomas. Vascular density is similar when FFH and FL interfollicular areas are compared.
Revista do Colégio Brasileiro de Cirurgiões, 2004
... Adedamola Ladipo1; Marcos Brasilino de Carvalho2; Abrão Rapoport, ECBC-SP2; Victor Eduardo Ar... more ... Adedamola Ladipo1; Marcos Brasilino de Carvalho2; Abrão Rapoport, ECBC-SP2; Victor Eduardo Arrua Arias3; Luiz Carlos Filgueiras Leiro1 ... Estudo anatomopatológico de timos de pa-cientes miastênicos, confirmou as alterações patológicas e verificou que 70% dos timos ...
Revista da Associação Médica Brasileira, 2004
OBJETIVO: O retinoblastoma é o tumor intra-ocular mais freqüente na infância. A forma de apresent... more OBJETIVO: O retinoblastoma é o tumor intra-ocular mais freqüente na infância. A forma de apresentação mais comum é leucocoria, seguida pelo estrabismo. Nos países em desenvolvimento, pela demora diagnóstica e maior tempo de encaminhamento, encontramos um grande número de pacientes portadores de tumores extra-oculares. MÉTODOS: Duzentos e trinta e nove pacientes com retinoblastoma admitidos nos departamentos de Pediatria e Oftalmologia do Hospital do Câncer AC Camargo, no período de 1986 a 1995, foram avaliados quanto ao tempo de encaminhamento e extensão do tumor. A análise estatística incluiu o cálculo de freqüências absolutas e relativas, bem como o teste exato de Fisher, para a verificação da associação entre variáveis categorias. RESULTADOS: Pacientes que foram encaminhados em um período menor que 6 meses apresentaram maior freqüência de doença intra-ocular quando comparados com pacientes com tempo de encaminhamento maior que seis meses (75% vs. 25% p<0,001). Não houve diferença estatisticamente significativa para tumores extra-oculares. CONCLUSÕES: O diagnóstico precoce é considerado o ponto mais importante no tratamento de pacientes com retinoblastoma. Os pediatras são capazes de detectar, em uma consulta simples de ambulatório, os sinais e sintomas desta doença e devem ter sempre em mente o diagnóstico de retinoblastoma, possibilitando o encaminhamento precoce a centros especializados.
Ultrasound in Medicine & Biology, 2010
This was a study on the effects of 3-MHz ultrasound at 16-and 100-Hz pulse repetition frequencies... more This was a study on the effects of 3-MHz ultrasound at 16-and 100-Hz pulse repetition frequencies on angiogenesis and viability of random-pattern skin flaps in rats. A cranially-based dorsal skin flap was raised in 60 EPM-Wistar rats, which were randomly divided into four groups: control, sham, 16-Hz and 100-Hz groups. The mean percentage of necrosis was as follows: control, 42% ± 13%; sham, 18% ± 13%; 16-Hz group, 13% ± 10%; and 100-Hz group, 15% ± 7%, with significant differences between the control and the other groups (p , 0.001). The mean vascular density was as follows: control, 5% ± 2%; sham, 7% ± 2%; 16-Hz group, 21% ± 4%; and 100-Hz group, 24% ± 10%, with significant differences between control and ultrasound groups, and between the sham and ultrasound groups (p , 0.001). Both ultrasound treatments (16-and 100-Hz PRFs) induced angiogenesis, and sham and ultrasound treatments improved viability of random-pattern skin flaps in rats.
Plastic and Reconstructive Surgery, 2010
The transverse rectus abdominis musculocutaneous (TRAM) flap may develop necrosis, especially in ... more The transverse rectus abdominis musculocutaneous (TRAM) flap may develop necrosis, especially in patients with risk factors such as previous abdominoplasty, caused by damage to perforating vessels during surgical procedures. This study was designed from the perspective of using vascular endothelial growth factor (VEGF) gene therapy with plasmid vector after abdominoplasty to stimulate neovascularization of the TRAM flap, thus increasing flap viability. Thirty-two Wistar rats were divided into four groups (n = 8). A right inferiorly based TRAM flap was constructed in all animals and was the only procedure performed in group I (TRAM flap). Animals from groups II (abdominoplasty) and III (plasmid) underwent abdominoplasty and were injected intramuscularly with physiologic saline solution and empty plasmid, respectively. Group IV (VEGF) received intramuscular injection of naked plasmid DNA encoding VEGF-165 during abdominoplasty. The TRAM flap was created 30 days after abdominoplasty. The mean necrosis was 24.65 +/- 18.13 percent in group I, 62.49 +/- 28.06 percent in group II, 57.80 +/- 25.43 percent in group III, and 18.33 +/- 16.20 percent in group IV. The number of vessels in the TRAM flap was determined by immunohistochemistry using the antibody human heart factor. Groups I and IV had a similar number of vessels, as did groups II and III. Groups I and IV had greater viability and number of vessels than groups II and III. VEGF gene therapy increased viability and vessel number in the TRAM flap created after abdominoplasty in a rat model.
Photomedicine and Laser Surgery, 2010
To assess the effects of low-level laser therapy (LLLT) with output power of 30 and 60 mW in the ... more To assess the effects of low-level laser therapy (LLLT) with output power of 30 and 60 mW in the viability of a random skin flap in rats. Output power values in LLLT are not well defined. Controlled, single-blind experimental study. Thirty-six animals were randomly distributed into three groups: sham group (SG), 30-mW output power (30G), and 60-mW output power (60G). In both treated groups, a fluency of 3 J/cm(2) was used. Two minutes after elevation of a random-pattern cranially based dorsal flap (4 x 10 cm), laser irradiation was applied and repeated on the first, second, third, and fourth postoperative days. Percentages of flap necrosis were calculated on the seventh postoperative day. Also, four fragments of each flap were collected to allow determination of the percentages of vascular density according to the bidimensional method of the morphometric analysis of blood vessels. Statistical analysis included the Wilcoxon test and Kruskal-Wallis variance analysis. A significance level of 5% was elected (p < 0.05). Laser-treated animals presented significantly less necrosis than the sham group (SG, 53%; 30G, 24%; p < 0.05) (60G, 25%; p < 0.05). Also, laser-treated animals presented significantly more vascular density than the sham group (SG, 37%; 30G, 57%; p < 0.05) (60G, 59%; p < 0.05). LLLT (660 nm) with 30-mW and 60-mW output power was efficient in the increase of skin flap viability, but there was no difference between them.
Pediatric Blood & Cancer, 2007
To assess the outcome and determine risk factors for extraocular relapse in patients with retinob... more To assess the outcome and determine risk factors for extraocular relapse in patients with retinoblastoma who had been enucleated after failure of chemoreduction. Retrospective study (1995-2002) at three institutions. Pathological risk factors (PRF) were defined as invasion of the anterior segment, choroid, post-laminar optic nerve, subarachnoid space, or sclera according to the local pathology report. Extraocular relapse was defined as an event. One hundred twenty-two patients were included (17 had bilateral enucleation). Chemoreduction included vincristine, carboplatin, and etoposide (n=80, 65.6%), vincristine, and carboplatin (n=17, 13.9%), or carboplatin (n=25, 20.5%). Thirty-five also received external beam radiotherapy (28.7%). PRF included: 39 with choroidal involvement, 9 with anterior segment, 9 with scleral, and 2 with post-laminar optic nerve with subarachnoid invasion. Adjuvant chemotherapy was given to eight patients (6.5%) because of scleral invasion. Four patients had an extraocular relapse after enucleation, two of whom survive after intensive treatment including stem cell rescue. Five-year probability of event-free survival is 0.96. Only scleral invasion and bilateral enucleation were significantly associated with extraocular relapse. The risk of extraocular relapse is low after enucleation following failure of chemoreduction. Patients who underwent bilateral enucleation and those with scleral invasion are at higher risk of extraocular relapse.
Pediatric Blood & Cancer, 2007
Retinoblastoma (RB) is the most common intraocular malignancy found in children. It is caused by ... more Retinoblastoma (RB) is the most common intraocular malignancy found in children. It is caused by the inactivation of both copies of a tumor suppressor gene (Rb1), which participates in the control of cell cycling (1-3). Approximately one-third of these tumors are bilateral and is associated with germinal mutations. All bilateral tumors and one tenth of unilateral tumors are caused by a germline mutation inherited as an autosomal dominant trait (4, 5). This hereditary predisposition to retinoblastoma is caused by mutant alleles occurring at the q14 band of chromosome 13 (6). The association of bilateral RB with ectopic midline intracranial tumors, termed "trilateral"retinoblastoma (TRB), is a well recognized but uncommon syndrome. The association of ocular RB with brain tumors was first reported in 1971 (7). The intracranial tumor arises most often in the pineal region but can also be a suprasellar or parasellar tumor, and is considered to be an isolated independent primary focus without evidence of retinal disease. This is the first reported case of TRB presented with a sellar and suprasellar tumor in Korea.
Pediatric Blood & Cancer, 2007
Retinoblastoma is a malignant tumor of the embryonic neural retina. About 80% of cases are diagno... more Retinoblastoma is a malignant tumor of the embryonic neural retina. About 80% of cases are diagnosed before age 4, with a median age at diagnosis of 2 years. To determine characteristics and prognosis of retinoblastoma in children older than 5 years. From 1986 to 2002, medical records of 16 patients out of 453 cases referred to Hospital do Câncer AC Camargo, São Paulo, Brazil. Median age at diagnosis was 73.7 months (range 65-144) and there was an equal gender distribution. Fifteen patients presented with unilateral disease. The mean time between first symptoms and diagnosis was 9.6 months (range 0-48). Most cases were diagnosed in advanced stages and 15 eyes were enucleated. Eleven patients presented with intraocular tumor (1 Reese II and 10 Reese V) and five presented with extraocular disease (one CCG II and four CCG III). Twelve patients are still alive with a median follow-up of 92 months (range 65-199). Because of its low incidence at this age, diagnosis of retinoblastoma is usually delayed due to low level of suspicion. Therefore, it is important that physicians are aware of this disease in order to perform an earlier diagnosis, and decrease treatment-related morbidity.
Medical and Pediatric Oncology, 1999
Rev Assoc Med Bras 2004; 50(4): 400-2 400 ANTONELI CBG ET AL. RESUMO -OBJETIVO. O retinoblastoma ... more Rev Assoc Med Bras 2004; 50(4): 400-2 400 ANTONELI CBG ET AL. RESUMO -OBJETIVO. O retinoblastoma é o tumor intra-ocular mais freqüente na infância. A forma de apresentação mais comum é leucocoria, seguida pelo estrabismo. Nos países em desenvolvimento, pela demora diagnóstica e maior tempo de encaminhamento, encontramos um grande número de pacientes portadores de tumores extra-oculares. MÉTODOS. Duzentos e trinta e nove pacientes com retinoblastoma admitidos nos departamentos de Pediatria e Oftalmologia do Hospital do Câncer AC Camargo, no período de 1986 a 1995, foram avaliados quanto ao tempo de encaminhamento e extensão do tumor. A análise estatística incluiu o cálculo de freqüências absolutas e relativas, bem como o teste exato de Fisher, para a verificação da associação entre variáveis categorias. RESULTADOS. Pacientes que foram encaminhados em um
Revista Da Associacao Medica Brasileira, 2004
OLIVEIRA AB ET AL.
Leukemia and Lymphoma, 2000
Tumor angiogenesis is required for tumor growth and metastasis. A statistically significant corre... more Tumor angiogenesis is required for tumor growth and metastasis. A statistically significant correlation has been demonstrated between prognosis and the microvessel density (a measure of tumor angiogenesis) of solid tumors, particularly of the breast and prostate, and lymphoid neoplasms. The aim of this study was to establish whether a correlation exists between vascular density and the malignant category of Non-Hodgkin's Lymphoma (NHL) defined by two classification systems (Kiel and Working Formulation). We also tested whether florid follicular hyperplasia (FFH) and follicular lymphomas (FL) behave as new vessel stimulating conditions. Eighty-nine NHL lymph node biopsies were reviewed and categorized according to the Kiel Classification and Working Formulation. Twelve FL were also selected and compared to 12 FFH biopsies. Vessels were highlighted by immunostaining with anti-Factor VIII antibody and quantified both by counting higher vascular density fields and by estimating the proportional vascular area. The results showed a statistically significant difference between low and high grade NHL, when classified in either the Working Formulation (p=0.0015) or the Kiel Classification (p=0.002). No differences were found in vessel counts between Working formulation intermediate and high grade lymphomas. Vascular density is similar when FFH and FL interfollicular areas are compared.
Journal of Plastic, Reconstructive & Aesthetic Surgery, 2010
Background: The angiogenic induction property of the omentum makes it a promising pedicle for pre... more Background: The angiogenic induction property of the omentum makes it a promising pedicle for prefabricating flaps. Therefore, the objective of this paper is to establish the abdominal area to be prefabricated by the omental pedicle and to analyse the prefabricated potential (PP) according to the time delay between the pedicle introduction and the flap release. Methods: Forty-four rabbits were divided into four groups (A, B, C and D). In group A, a piece of skin, subcutaneous tissue and abdominal cutaneous muscle was fully released and sutured again in place. In the other groups, a 9-cm 2 omental pedicle containing the gastromental vessels distally tied was transposed and sutured to abdominal cutaneous muscle. A second procedure, consisting of incision and release of the flap that contained skin, subcutaneous and cutaneous abdominal muscle pediculated only by the omentum, was carried out. The only variation was the time delay between the two procedures: 7, 21 and 56 days for groups B, C and D, respectively. The flaps were inspected 15 days after the last procedure. The pieces of viable area were immunostained using anti-CD31 for estimation of the microvascular density. Results: The mean and maximum viable areas in group D were 45.29 and 99.37 cm 2 , respectively (average PP Z 5.03 and maximum PP Z 11.04). There was no significant difference between the viable areas in groups C and D. The mean microvascular densities of groups B, C and D were 24.54, 33.20 and 27.03 vessels/mm 2 , respectively.
Journal of Pediatric Hematology/Oncology, 2007
This study aimed to determine the impact of the addition of ifosfamide/etoposide to a regimen con... more This study aimed to determine the impact of the addition of ifosfamide/etoposide to a regimen containing cisplatin/teniposide on the survival of patients with retinoblastoma with orbital involvement. Thirty patients were treated at the A. C. Camargo Hospital, Brazil, from 1986 to 2002. From 1986 to April 1992 (period I, n=12), treatment consisted of 3 cycles of induction chemotherapy with cisplatin and teniposide, followed by maintenance with same drugs alternating with cyclophosphamide, vincristine, and doxorubicin every 21 days for 60 weeks. Since April 1992 (period II, n=18), the treatment consisted of 3 cycles of ifosfamide and etoposide followed by maintenance with same drugs, alternating with cisplatin and teniposide every 21 days for 36 weeks. In both periods, children were submitted to exenteration with eyelid preservation and orbital radiation therapy with 45 cGy, and also received intrathecal therapy with methotrexate plus dexamethasone and cytarabine. Kaplan-Meier method was used for survival analysis. The median age was 31 months. Most patients (86.7%) presented unilateral tumors. The 3-year overall survival was 34.4% and 72.2%, respectively, for patients treated during periods I and II (P=0.061). The addition of ifosfamide/etoposide to chemotherapy with cisplatin/teniposide improves survival in these patients, but further studies are still necessary.
Clinical Nutrition, 2008
Conclusions: LE, independently of composition, had no influence on neutrophils' chemotaxis, but s... more Conclusions: LE, independently of composition, had no influence on neutrophils' chemotaxis, but showed different effect on phagocytosis by macrophages. SMOF LE had neutral effect while fish oil LE enriched with MCT/LCT LE increased resident-macrophages' phagocytosis. ª