Comparison of a dipstick enzyme-linked immunosorbent assay with

commercial assays for detection of Japanese encephalitis virus-specific IgM antibodies (original) (raw)

Files

Journal Title

Journal ISSN

Volume Title

Publisher

Medknow Publications and Staff Society of Seth GS Medical College and KEM Hospital, Mumbai, India

Abstract

Description

Background: Japanese encephalitis (JE) is a major public health concern in Asia including India. Objectives: To evaluate an in-house developed dipstick enzyme-linked immunosorbent assay (ELISA) test vis-à-vis two commercial kits for detection of JE virus-specific IgM antibodies. Setting and Design: Comparative study carried out in Research and Development centre. Materials and Methods: A total of 136 specimens comprising 84 serum and 52 CSF samples were tested by in-house dipstick ELISA, Pan-Bio IgM capture ELISA (Pan-Bio, Australia) and JEV CheX IgM capture ELISA (XCyton, India). Results: The overall agreement among all three tests was found to be 92% with both serum and cerebrospinal fluid (CSF) samples. The sensitivity of the dipstick ELISA was found to be 91% with serum and 89% with CSF samples respectively. The specificity of the dipstick ELISA with reference to both commercial assays was found to be 100% in serum and CSF samples in this study. Conclusions: The in-house dipstick ELISA with its comparable sensitivity and specificity can be used as a promising test in field conditions since it is simple, rapid and requires no specialized equipment.

Keywords

virus

,

Japanese encephalitis

,

dipstick enzyme-linked immunosorbent assay

,

diagnostic test

,

Antibody

Citation

DOI

ISSN

Creative Commons

Creative Commons URI

Collections

Items in TSpace are protected by copyright, with all rights reserved, unless otherwise indicated.