dr Dewi Wulandari 197009051997022002 | Universitas Indonesia ui (original) (raw)

Papers by dr Dewi Wulandari 197009051997022002

Adipose tissue derived stem cells (ASCs) are very promising for regenerative medicine. However, b... more Adipose tissue derived stem cells (ASCs) are very promising for regenerative medicine. However, before the stem cells can be used to treat patients, they need to be propagated in animal serum-free materials and should be characterized. Therefore, to know the characteristics of ASCs that were grown in various animal serum-free medium, we cultured the ASCs in various media, i.e. 5% or 10% human AB platelet rich plasma (PRP), or combination of vascular endothelial growth factor (VEGF) and human AB serum containing Dulbecco’s modified Eagle medium (DMEM), and conducted flow cytometry analysis; then, we compared them with those that were grown in the fetal bovine serum (FBS)-derivate containing commercial medium (MesenCult®). Our results showed that the grown cells in the four kinds of medium had different proportions of CD34, CD73 and CD90 positive cells. Cells that were grown in 5% and 10% PRP containing media were comparable to those that were grown in MesenCult®, and could be regarded as adipose tissue-derived mesenchymal stem cells (AT-MSCs), while VEGF and human AB serum containing medium might preserve and promote the CD34 positive cells, but at the same time differentiated a proportion of AT-MSCs into chondrogenic cells. In conclusion, flow cytometric analysis showed that different media caused different proprortions of surface marker expressions in AT-MSCs

The Indonesian Biomedical Journal, 2022

BACKGROUND: Dengue infection remains a major public health problem in Indonesia. Severe dengue as... more BACKGROUND: Dengue infection remains a major public health problem in Indonesia. Severe dengue associated with plasma leakage require hospitalization and potentially life threatening. However, the mechanism remains unclear, and the occurrence is unpredictable. The role of anti-endothelial antibody is predicted play an important role in the pathogenesis of plasma leakage, as severe dengue is more prevalent in secondary infection or post vaccinated individuals.METHODS: Serum samples from 127 single Dengue Virus (DENV) serotype infected subjects were obtained in day 2 of fever onset. Subjects were divided into plasma leakage and non-plasma leakage based on World Health Organization (WHO) criteria. Anti-endothelial antibody in patient sera were detected using western blot of Human Umbilical Vein Endothelial Cells (HUVEC). To confirm cross-reactivity, the sera was preabsorb with mix-DENV lysate.RESULTS: Three prominent bands were identified on western blot strips that inhibited by pre-ab...

Neurology. Clinical practice, 2018

Little detailed knowledge is available regarding the etiology and outcome of CNS infection, parti... more Little detailed knowledge is available regarding the etiology and outcome of CNS infection, particularly in HIV-infected individuals, in low-resource settings. From January 2015 to April 2016, we prospectively included all adults with suspected CNS infection in a referral hospital in Jakarta, Indonesia. Systematic screening included HIV testing, CSF examination, and neuroimaging. A total of 274 patients with suspected CNS infection (median age 26 years) presented after a median of 14 days with headache (77%), fever (78%), seizures (27%), or loss of consciousness (71%). HIV coinfection was common (54%), mostly newly diagnosed (30%) and advanced (median CD4 cell count 30/µL). Diagnosis was established in 167 participants (65%), including definite tuberculous meningitis (TBM) (n = 44), probable TBM (n = 48), cerebral toxoplasmosis (n = 48), cryptococcal meningitis (n = 14), herpes simplex virus/varicella-zoster virus/cytomegalovirus encephalitis (n = 10), cerebral lymphoma (n = 1), neu...

Oto Rhino Laryngologica Indonesiana

Latar belakang: Rinitis alergi adalah suatu penyakit inflamasi pada hidung yang dimediasi oleh i... more Latar belakang: Rinitis alergi adalah suatu penyakit inflamasi pada hidung yang dimediasi oleh imunoglobulin-E, yang terjadi setelah mukosa hidung terpajan alergen. Sel dan mediator inflamasi yang berperan penting adalah eosinofil, histamin, dan sitokin produk Th2. Penyakit ini ditandai dengan gejala rinorea, bersin berulang, hidung tersumbat dan/atau hidung gatal. Selain dari gejala yang mengganggu, rinitis alergi berdampak buruk terhadap kualitas hidup penderita. Tatalaksana komprehensif, meliputi penghindaran alergen, farmakoterapi, imunoterapi dan edukasi, ditujukan untuk menghilangkan gejala dan memperbaiki kualitas hidup pasien. Tujuan: Mengetahui perubahan kualitas hidup, jumlah eosinofil mukosa hidung, dan kadar IL-5 serum pada pasien rinitis alergi setelah mendapat terapi farmaka sesuai panduan. Metode: Penelitian ini merupakan studi pre-eksperimental analitik dengan pengambilan data sebelum dan sesudah pemberian farmakoterapi. Hasil: Sebanyak 31 dari 33 subjek mengalami p...

Neurology. Clinical practice, 2018

Little detailed knowledge is available regarding the etiology and outcome of CNS infection, parti... more Little detailed knowledge is available regarding the etiology and outcome of CNS infection, particularly in HIV-infected individuals, in low-resource settings. From January 2015 to April 2016, we prospectively included all adults with suspected CNS infection in a referral hospital in Jakarta, Indonesia. Systematic screening included HIV testing, CSF examination, and neuroimaging. A total of 274 patients with suspected CNS infection (median age 26 years) presented after a median of 14 days with headache (77%), fever (78%), seizures (27%), or loss of consciousness (71%). HIV coinfection was common (54%), mostly newly diagnosed (30%) and advanced (median CD4 cell count 30/µL). Diagnosis was established in 167 participants (65%), including definite tuberculous meningitis (TBM) (n = 44), probable TBM (n = 48), cerebral toxoplasmosis (n = 48), cryptococcal meningitis (n = 14), herpes simplex virus/varicella-zoster virus/cytomegalovirus encephalitis (n = 10), cerebral lymphoma (n = 1), neu...

The Indonesian Biomedical Journal

BACKGROUND: Syphilis and yaws are the treponemal infections which have become serious public heal... more BACKGROUND: Syphilis and yaws are the treponemal infections which have become serious public health problems, and both are serologically indistinguishable. Developed serological tests for syphilis may also be used to diagnose yaws. In remote area, test modality with minimal requirements were needed. This study investigated the diagnostic value of syphilis rapid diagnostic test (RDT) in diagnosing syphilis and yaws.METHODS: For syphilis diagnostic test, serum samples were obtained from patients of outpatient clinic in Dr. Cipto Mangunkusumo National Central General Hospital who were sent for rapid plasma reagin (RPR) and Treponema pallidum haemagglutination assay (TPHA) to clinical laboratory of the hospital. The serum samples were collected and stored at -80°C until the day of testing for syphilis RDT and fluorescent treponemal antibody-absorption (FTA-Abs). For yaws diagnostic test, serum samples were obtained as a part of surveillance study of yaws among children 1-15 years old in...

The New Ropanasuri : Journal of Surgery

Medical Journal of Indonesia

Avicenna journal of medical biotechnology

Recently, Phosphatidylcholine (PC) has been used as an off-label treatment for lipolysis injectio... more Recently, Phosphatidylcholine (PC) has been used as an off-label treatment for lipolysis injection, which is associated with inflammatory reaction due to sodium deoxycholate, an emulsifier, so that inflammation as side effect occurs in those patients. Liposome formulation from lipid was thought to be a better and safer alternative. This study aimed to analyze the mechanism of Liposomal Soybean Phosphatidylcholine (LSPC) extract from Indonesian soybeans (containing 26% PC) to induce Adipose-derived Stem Cells (ASCs) death . Liposomes were prepared using thin film hydration method followed by a stepwise extrusion process to produce a small amount of 41.0-71.3 Liposomal soybean phosphatidylcholine extract (LSPCE), liposomal purified PC (LPCC), and solution of PC+SD were used for comparison. Annexin V fluorescein Isothiocyanate/Propidium Iodide (FITC/PI) double staining by flow cytometry and also measurement of caspase-3 activity using ELISA were used to quantify the rate of apoptosis. ...

OnLine Journal of Biological Sciences, 2015

Flowcytometric analysis is widely used in characterization of stem cells. In areas where flowcyto... more Flowcytometric analysis is widely used in characterization of stem cells. In areas where flowcytometry is not available, characterization of stem cells can be done using immunocytochemistry. This study aimed to compare the results of flowcytometry against immunocytochemistry for the measurement of CD34, CD73 and CD90 in stem cells. Adipose tissue derived stem cells were analyzed for their CD34, D73 and CD90 simultaneously, using a BD FACSCalibur flowcytometer (BD Biosciences) with 10,000 gated events. The same sample was made into spot specimens and checked for CD34, CD73 and CD90 by immunocytochemistry and when available, 100 cells were screened for positive results and percentages of positive results for CD34, CD73 and CD90 were computed. The results of the two methods were compared. Discrepancies between the two methods were noted for all samples. Discrepancies lower than 3, 11 and 11% for CD34, CD73 and CD90 respectively were regarded as agreement between the two methods. Percentages of agreement in CD34, CD73 and CD90 were calculated. Some spot specimens did not yield 100 cells and analyses were done on available cells. The agreement between the two methods for CD34, CD73 and CD90 were 22, 42 and 29% respectively. Agreement between flowcytometry and immunocytochemistry was low for CD34 and CD90 and was moderate for CD73.

Adipose tissue derived stem cells (ASCs) are very promising for regenerative medicine. However, b... more Adipose tissue derived stem cells (ASCs) are very promising for regenerative medicine. However, before the stem cells can be used to treat patients, they need to be propagated in animal serum-free materials and should be characterized. Therefore, to know the characteristics of ASCs that were grown in various animal serum-free medium, we cultured the ASCs in various media, i.e. 5% or 10% human AB platelet rich plasma (PRP), or combination of vascular endothelial growth factor (VEGF) and human AB serum containing Dulbecco’s modified Eagle medium (DMEM), and conducted flow cytometry analysis; then, we compared them with those that were grown in the fetal bovine serum (FBS)-derivate containing commercial medium (MesenCult®). Our results showed that the grown cells in the four kinds of medium had different proportions of CD34, CD73 and CD90 positive cells. Cells that were grown in 5% and 10% PRP containing media were comparable to those that were grown in MesenCult®, and could be regarded as adipose tissue-derived mesenchymal stem cells (AT-MSCs), while VEGF and human AB serum containing medium might preserve and promote the CD34 positive cells, but at the same time differentiated a proportion of AT-MSCs into chondrogenic cells. In conclusion, flow cytometric analysis showed that different media caused different proprortions of surface marker expressions in AT-MSCs

The Indonesian Biomedical Journal, 2022

BACKGROUND: Dengue infection remains a major public health problem in Indonesia. Severe dengue as... more BACKGROUND: Dengue infection remains a major public health problem in Indonesia. Severe dengue associated with plasma leakage require hospitalization and potentially life threatening. However, the mechanism remains unclear, and the occurrence is unpredictable. The role of anti-endothelial antibody is predicted play an important role in the pathogenesis of plasma leakage, as severe dengue is more prevalent in secondary infection or post vaccinated individuals.METHODS: Serum samples from 127 single Dengue Virus (DENV) serotype infected subjects were obtained in day 2 of fever onset. Subjects were divided into plasma leakage and non-plasma leakage based on World Health Organization (WHO) criteria. Anti-endothelial antibody in patient sera were detected using western blot of Human Umbilical Vein Endothelial Cells (HUVEC). To confirm cross-reactivity, the sera was preabsorb with mix-DENV lysate.RESULTS: Three prominent bands were identified on western blot strips that inhibited by pre-ab...

Neurology. Clinical practice, 2018

Little detailed knowledge is available regarding the etiology and outcome of CNS infection, parti... more Little detailed knowledge is available regarding the etiology and outcome of CNS infection, particularly in HIV-infected individuals, in low-resource settings. From January 2015 to April 2016, we prospectively included all adults with suspected CNS infection in a referral hospital in Jakarta, Indonesia. Systematic screening included HIV testing, CSF examination, and neuroimaging. A total of 274 patients with suspected CNS infection (median age 26 years) presented after a median of 14 days with headache (77%), fever (78%), seizures (27%), or loss of consciousness (71%). HIV coinfection was common (54%), mostly newly diagnosed (30%) and advanced (median CD4 cell count 30/µL). Diagnosis was established in 167 participants (65%), including definite tuberculous meningitis (TBM) (n = 44), probable TBM (n = 48), cerebral toxoplasmosis (n = 48), cryptococcal meningitis (n = 14), herpes simplex virus/varicella-zoster virus/cytomegalovirus encephalitis (n = 10), cerebral lymphoma (n = 1), neu...

Oto Rhino Laryngologica Indonesiana

Latar belakang: Rinitis alergi adalah suatu penyakit inflamasi pada hidung yang dimediasi oleh i... more Latar belakang: Rinitis alergi adalah suatu penyakit inflamasi pada hidung yang dimediasi oleh imunoglobulin-E, yang terjadi setelah mukosa hidung terpajan alergen. Sel dan mediator inflamasi yang berperan penting adalah eosinofil, histamin, dan sitokin produk Th2. Penyakit ini ditandai dengan gejala rinorea, bersin berulang, hidung tersumbat dan/atau hidung gatal. Selain dari gejala yang mengganggu, rinitis alergi berdampak buruk terhadap kualitas hidup penderita. Tatalaksana komprehensif, meliputi penghindaran alergen, farmakoterapi, imunoterapi dan edukasi, ditujukan untuk menghilangkan gejala dan memperbaiki kualitas hidup pasien. Tujuan: Mengetahui perubahan kualitas hidup, jumlah eosinofil mukosa hidung, dan kadar IL-5 serum pada pasien rinitis alergi setelah mendapat terapi farmaka sesuai panduan. Metode: Penelitian ini merupakan studi pre-eksperimental analitik dengan pengambilan data sebelum dan sesudah pemberian farmakoterapi. Hasil: Sebanyak 31 dari 33 subjek mengalami p...

Neurology. Clinical practice, 2018

Little detailed knowledge is available regarding the etiology and outcome of CNS infection, parti... more Little detailed knowledge is available regarding the etiology and outcome of CNS infection, particularly in HIV-infected individuals, in low-resource settings. From January 2015 to April 2016, we prospectively included all adults with suspected CNS infection in a referral hospital in Jakarta, Indonesia. Systematic screening included HIV testing, CSF examination, and neuroimaging. A total of 274 patients with suspected CNS infection (median age 26 years) presented after a median of 14 days with headache (77%), fever (78%), seizures (27%), or loss of consciousness (71%). HIV coinfection was common (54%), mostly newly diagnosed (30%) and advanced (median CD4 cell count 30/µL). Diagnosis was established in 167 participants (65%), including definite tuberculous meningitis (TBM) (n = 44), probable TBM (n = 48), cerebral toxoplasmosis (n = 48), cryptococcal meningitis (n = 14), herpes simplex virus/varicella-zoster virus/cytomegalovirus encephalitis (n = 10), cerebral lymphoma (n = 1), neu...

The Indonesian Biomedical Journal

BACKGROUND: Syphilis and yaws are the treponemal infections which have become serious public heal... more BACKGROUND: Syphilis and yaws are the treponemal infections which have become serious public health problems, and both are serologically indistinguishable. Developed serological tests for syphilis may also be used to diagnose yaws. In remote area, test modality with minimal requirements were needed. This study investigated the diagnostic value of syphilis rapid diagnostic test (RDT) in diagnosing syphilis and yaws.METHODS: For syphilis diagnostic test, serum samples were obtained from patients of outpatient clinic in Dr. Cipto Mangunkusumo National Central General Hospital who were sent for rapid plasma reagin (RPR) and Treponema pallidum haemagglutination assay (TPHA) to clinical laboratory of the hospital. The serum samples were collected and stored at -80°C until the day of testing for syphilis RDT and fluorescent treponemal antibody-absorption (FTA-Abs). For yaws diagnostic test, serum samples were obtained as a part of surveillance study of yaws among children 1-15 years old in...

The New Ropanasuri : Journal of Surgery

Medical Journal of Indonesia

Avicenna journal of medical biotechnology

Recently, Phosphatidylcholine (PC) has been used as an off-label treatment for lipolysis injectio... more Recently, Phosphatidylcholine (PC) has been used as an off-label treatment for lipolysis injection, which is associated with inflammatory reaction due to sodium deoxycholate, an emulsifier, so that inflammation as side effect occurs in those patients. Liposome formulation from lipid was thought to be a better and safer alternative. This study aimed to analyze the mechanism of Liposomal Soybean Phosphatidylcholine (LSPC) extract from Indonesian soybeans (containing 26% PC) to induce Adipose-derived Stem Cells (ASCs) death . Liposomes were prepared using thin film hydration method followed by a stepwise extrusion process to produce a small amount of 41.0-71.3 Liposomal soybean phosphatidylcholine extract (LSPCE), liposomal purified PC (LPCC), and solution of PC+SD were used for comparison. Annexin V fluorescein Isothiocyanate/Propidium Iodide (FITC/PI) double staining by flow cytometry and also measurement of caspase-3 activity using ELISA were used to quantify the rate of apoptosis. ...

OnLine Journal of Biological Sciences, 2015

Flowcytometric analysis is widely used in characterization of stem cells. In areas where flowcyto... more Flowcytometric analysis is widely used in characterization of stem cells. In areas where flowcytometry is not available, characterization of stem cells can be done using immunocytochemistry. This study aimed to compare the results of flowcytometry against immunocytochemistry for the measurement of CD34, CD73 and CD90 in stem cells. Adipose tissue derived stem cells were analyzed for their CD34, D73 and CD90 simultaneously, using a BD FACSCalibur flowcytometer (BD Biosciences) with 10,000 gated events. The same sample was made into spot specimens and checked for CD34, CD73 and CD90 by immunocytochemistry and when available, 100 cells were screened for positive results and percentages of positive results for CD34, CD73 and CD90 were computed. The results of the two methods were compared. Discrepancies between the two methods were noted for all samples. Discrepancies lower than 3, 11 and 11% for CD34, CD73 and CD90 respectively were regarded as agreement between the two methods. Percentages of agreement in CD34, CD73 and CD90 were calculated. Some spot specimens did not yield 100 cells and analyses were done on available cells. The agreement between the two methods for CD34, CD73 and CD90 were 22, 42 and 29% respectively. Agreement between flowcytometry and immunocytochemistry was low for CD34 and CD90 and was moderate for CD73.