Ratendra Kumar | HNB Garhwal University (original) (raw)

Papers by Ratendra Kumar

Salvia officinalis is a Ayurvedic traditional medicinal plant in India and is considered useful i... more Salvia officinalis is a Ayurvedic traditional medicinal plant in India and is considered useful in various ailments. Preliminary neuropsychopharmacological evaluation revealed cognition enhancing ability of the flower extract of the plant. Therefore, the present study aims at evaluating the potential effectiveness of the methanol extract of the flowers of this plant in dementia related to Alzheimer’s disease (AD). Dementia was induced experimentally in animals by ingesting excess Copper and Aluminium in drinking water and confirmed by Radial 8 arm maze test. Differentl biochemical parameters including superoxide dismutase (SOD), glutathione-oxidized (GSSG), reduced glutathione (GSH), nitrite estimation, myeloperoxidase (MPO) activity and acetylcholinesterase (AChE) activity were also estimated. Lipid profile was also evaluated along with the histopathological observations. Results demonstrated a neuroprotective profile of the methanol extract of the flowers of Salvia officinalis as ...

International Journal of Diabetes Mellitus, 2015

Aim of the study: In the present study, we examined and compared the effect of Polyherbal (PH), A... more Aim of the study: In the present study, we examined and compared the effect of Polyherbal (PH), Allopolyherbal-A (APH-A), Allopolyherbal-B (APH-B), and Allopolyherbal-C (APH-C) formulations on hyperglycemia, lipid profile, renal, and hepatic function in streptozotocin (STZ) induced diabetes mellitus in rats. Materials and methods: The hypoglycemic activity (along with other parameters) of Polyherbal and Allopolyherbal formulations was investigated in STZ induced diabetes in rats. Polyherbal (PH) (3.63 g/kg body wt.); Allopolyherbal-A (APH-A) [(5 mg Gliclazide + 1.81 g PH)/kg body wt.]; Allopolyherbal-B (APH-B) [(4 mg Gliclazide + 2.17 g of PH)/kg body wt.]; Allopolyherbal-C (APH-C) [(2 mg of Gliclazide + 2.904 g of PH)/kg body wt.], and Gliclazide (10 mg/kg body wt.) were administered once a day, orally by gavages for 21 days. Blood glucose levels were measured on 0, 7, 14, and 21 days of the study; total cholesterol, triglycerides, LDL, VLDL, HDL, serum creatinine, SGOT, and SGPT were estimated on 21st day.

Journal of Acute Disease, 2013

The aim of the present study was to evaluate the hepatoprotective effect of Prunus persica leaves... more The aim of the present study was to evaluate the hepatoprotective effect of Prunus persica leaves extract against carbon tetrachloride induced hepatic injury in rats. Animals in Group I served as normal control (distilled water) group, Group II served as toxic control (CCl4 treated ) group, Group III served as standard (Silymarin) group, and Group IV, V served as ( 200 & 400 mg/kg bw, p.o) Prunus persica leaves extract treated groups respectively. The levels of the serum biomarkers such as serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), bilirubin, albumin & total protein were significantly increased in CCl4 treated rats when compared with the normal control group but the rats treated with Prunus persica leaves extract (200 mg/kg ) showed decrease in SGOT (250.5±4.43), SGPT (176.4±5.20), ALP (242.43±6.30), bilirubin (2.20±0.05) level and increase in serum albumin (3.20±0.05) and total protein (3.81±0.07) leve...

Despite progress made in modern medical science herbal drugs constitute a major share of all the ... more Despite progress made in modern medical science herbal drugs constitute a major share of all the officially recognized systems of health in India viz. Ayurveda, Yoga, Unani, Siddha, Homeopathy and Naturopathy. More than 70% of India’s 1.1 billion populations still use these non-allopathic systems of medicine. Various synthetic drugs like Sildenafil citrate, Tadalafil citrate, Vardenafil, etc are used to treat ED but these drugs also have fatal side effects. The use of synthetic aphrodisiacs results in the dilation of blood vessels in other parts of the body causing headache and fainting. Other side effects include facial flushing, stomach upset, blurred vision and sensitivity to light which usually occur at higher doses. Thus, there is growing need to look for aphrodisiacs more of herbal origin as opposed to synthetic compounds which are known to cause severe unwanted side effects. Unlike the old-time aphrodisiacs, which were meant only to increase sex drive and/or sexual pleasure, ...

Article history: The aim of this present work was to develop and optimize compression coated tabl... more Article history: The aim of this present work was to develop and optimize compression coated tablet of carvedilol sulphate for chronotherapeutic application by response surface methodology based on 3 2 factorial designs. Compression coated tablet containing carvedilol phosphate in the core was formulated with an outer coat by eudragit L 100 and ethyl cellulose. The percentage weight ratio of ethyl cellulose to eudragit L 100 and coating level were selected as critical process parameters (CPPs), whereas critical quality attributes (CQAs) were lag time and cumulative percentage drug release at 8 hr in current study. For optimization, the effects of critical process parameters upon the critical quality attributes were modelled using the polynomial equations involving critical process parameters and their interactions for various critical quality attributes. A numerical optimization technique was adopted to achieve optimized formulation which was also used as the check point.The observe...

Genetic variability and character associaton between yield and its contributing trait were studie... more Genetic variability and character associaton between yield and its contributing trait were studied in 12 varieties of cultivated lentil (Lens culanris Medik). Wide range of variability was observed for various characters. In general phenotypic coefficent of variation was higher ...

In the present investigation experimental design techniques was used for the preparation and opti... more In the present investigation experimental design techniques was used for the preparation and optimization of mouth dissolving film of salbutamol sulphate containing maltodextrin (MDX) and glycerine (Gly). A 3 2 factorial design was used to study the effect of amount of MDX (X1) and Gly (X2) on the responses: tensile strength (TS), elastic modulus (EM) and elongation at break (EB). Analysis of variance (ANOVA) was performed for different variable. The numerical optimization technique based on the desirability approaches was used to optimize oral fast dissolving film (OFDF). The optimized OFDF 10, which contained 55.0 % MDX and 19.99 % Gly, showed 0.25 MPa TS, 0.131 MPa EM and 113.25 % EB. The observed values were more identical to predicated value.

Life sciences, Jan 15, 2018

The present study aimed to evaluate the neuroprotective potential of intranasally administered pi... more The present study aimed to evaluate the neuroprotective potential of intranasally administered pitavastatin in the PTZ-induced kindling model. Subconvulsant dose of PTZ (35 mg/kg, i.p) was administered on an alternate day until the development of kindling. Behavioural test, biochemical tests and inflammatory cytokines were estimated. Comparative molecular docking study of sodium valproate (VPA) and pitavastatin was performed to predict the binding affinity with GABA and GABA transaminase. Intranasally administered pitavastatin (0.5 mg/kg and 1 mg/kg) and VPA (200 mg/kg) were used to investigate its protective effect. Comparative in-silico study showed docking score of -4.56 and -2.86 against GABA receptor whereas -5.56 and -1.86, against GABA transaminase. Root mean square deviation (RMSD) of 0.39A and 0.55A was found for pitavastatin and VPA, respectively. The present study showed the dose-dependent protective effect of intranasally administered pitavastatin and oral VPA against PT...

Journal of Applied Pharmaceutical Science

The aim of present study was to improve dissolution rate of olanzapine by means of solid dispersi... more The aim of present study was to improve dissolution rate of olanzapine by means of solid dispersion using combination of hydrophilic polymer (PEG & PVP) by using response surface design. Solid dispersion containing olanzapine were prepared using PEG 20000 & PVP K 30 by melted fusion method. Response surface method was used for the optimization olanzapine solid dispersions. Amount of PEG 20000 and Amount of PVP K 30 were selected as the critical process parameters (Independent variable) whereas amount dissolved in 10 minute (Q 10) and amount dissolved in 45 minute (Q 45) were selected as critical quality attributes (dependent variables). Optimized solid dispersion batch was characterized using infrared spectroscopy, differential scanning calorimetry (DSC) and X-ray diffractometry (XRD).Dissolution studies indicated a significant improvement in dissolution of olanzapine when dispersed in PEG 20000 and PVP k 30. XRD and DSC study indicated amorphous form of prepared solid dispersions. On the basis of numerical optimization technique, PEG 20000(X1) and amount of PVP K 30(X2) were 11.20 % and 14.53 % in optimized solid dispersion. The observed responses were closed well with the predicted values. The response surface method is found to be robust and accurate for optimization of solid dispersion for increase in solubility and dissolution rate of olanzapine, coherent with the needs of poorly water soluble drugs.

International Research Journal of Pharmacy, 2015

ABSTRACT Adhatoda vasica Nees (Family Acanthaceae), commonly known as Vasaka or Arusha is found c... more ABSTRACT Adhatoda vasica Nees (Family Acanthaceae), commonly known as Vasaka or Arusha is found commonly in India. In present investigation, pharmacognostical studies of leaves of Adhatoda vasica was carried out to lay down the standards which could be useful in future experimental studies.

Aim of the study was investigate the anti-inflammatory effect of various extracts of Fagonia schw... more Aim of the study was investigate the anti-inflammatory effect of various extracts of Fagonia schweinfurthii Hadidi on carrageenan induced paw-edema in rats. Carrageenan-induced paw edema inflammation model were used for the study. The antiinflammatory activity of various extracts of Fagonia schweinfurthii Hadidi were investigated in carrageenan induced paw-edema in rats. The extracts (500 mg/kg) and standard drugs (500mg/kg) were administered by oral route to each rat. After 30 minutes the inflammation was induced by single subcutaneous injection of 0.1% w/v carrageenan in the plantar region of paw of animals. The paw volume were measured immediately after the carrageenan injection and then at 30min, 1, 2, 3, and 4 hrs. The statistical significance of differences between groups was determined by analysis of variance (ANOVA), followed by Dunnett's test for multiple comparisons among groups. Differences of p<0.05 was considered statistically significant. Results showed that Methanolic and chloroform extracts produced 76.16% and 68.87% of inhibition of inflammation respectively; after 4 hrs at a dose of 500mg/kg. Further percent inhibition of inflammation by aqueous and ethyl acetate extracts were found to be 36.42% and 49.67% respectively at a dose of 500mg/kg. From the results of the study, it was concluded that among all the extracts, Methanolic and Chloroform extracts were most effective extracts and showed the statistically significant anti-inflammatory activity.

The aim of the study was undertaken to find out the release modifying potential of Prunus persica... more The aim of the study was undertaken to find out the release modifying potential of Prunus persica gum in the formulation of diclofenac sodium matrix tablets. Purified isolated gum was subjected to physicochemical characterization. Four formulations containing Prunus persica gum with each containing 100mg of Diclofenac sodium were prepared by wet granulation method using different drug: gum ratios viz. 1:0.25, 1:0.5, 1:1 and 1:2. Microcrystalline cellulose was used as diluent while magnesium stearate and talc were employed as lubricant and glidant respectively. The prepared formulations were evaluated for pre-compression parameters relevant to granules like angle of repose, bulk density, tapped density, hausner's index and carr's index while tablets were evaluated for various post-compression parameters like tablet thickness, hardness, weight variation, friability, content uniformity, disintegration time, swelling behaviour and in-vitro drug release study. All the formulation...

Der Pharmacia Sinica

Salvia officinalis is a Ayurvedic traditional medicinal plant in India and is considered useful i... more Salvia officinalis is a Ayurvedic traditional medicinal plant in India and is considered useful in various ailments. Preliminary neuropsychopharmacological evaluation revealed cognition enhancing ability of the flower extract of the plant. Therefore, the present study aims at evaluating the potential effectiveness of the methanol extract of the flowers of this plant in dementia related to Alzheimer's disease (AD). Dementia was induced experimentally in animals by ingesting excess Copper and Aluminium in drinking water and confirmed by Radial 8 arm maze test. Differentl biochemical parameters including superoxide dismutase (SOD), glutathione-oxidized (GSSG), reduced glutathione (GSH), nitrite estimation, myeloperoxidase (MPO) activity and acetylcholinesterase (AChE) activity were also estimated. Lipid profile was also evaluated along with the histopathological observations. Results demonstrated a neuroprotective profile of the methanol extract of the flowers of Salvia officinalis...

INTERNATIONAL JOURNAL OF ADVANCES IN PHARMACEUTICAL RESEARCH

The study was undertaken to evaluate the binding property of mucilage extracted from the Ficus pa... more The study was undertaken to evaluate the binding property of mucilage extracted from the Ficus palmata in the formulation of oral sustained release dosage form. Purified extracted mucilage was subjected to physicochemical characterization. Four formulations each containing 100mg of Diclofenac sodium were prepared by wet granulation method using different drug: mucilage ratios viz. 1:0.25, 1:0.5, 1:1 and 1:2. Microcrystalline cellulose was used as diluent while magnesium stearate and talc were employed as lubricant and glidant respectively. The prepared formulations were evaluated for pre-compression parameters like angle of repose, bulk density, tapped density, hausner's index and carr's index for granules while tablets were evaluated for various post-compression parameters like tablet thickness, hardness, weight variation, friability, content uniformity, disintegration time, swelling behaviour and in-vitro drug release study. All the formulations showed compliance with phar...

To evaluate the anti-inflammatory and antioxidant potential of methanol extract of flowers of Sal... more To evaluate the anti-inflammatory and antioxidant potential of methanol extract of flowers of Salvia officinalis Linn. (MESO). Antioxidant activity and free radical scavenging activity of MESO has been evaluated by employing various in vitro models including reducing power, Total antioxidant activity, DPPH radical, ABTS radical, superoxide radical (O 2 • –), nitric oxide, hydrogen peroxide, hydroxyl radical scavenging activity and metal chelating activity. Anti-inflammatory activity has been evaluated by carrageenan induced rat paw edema animal model. The plant extract (MESO) revealed significant antioxidant activity with lower IC 50 values. Metal chelating activity was found poor. In inflammation model, MESO demonstrated significant anti-inflammatory activity with maximum oedema inhibition at 3 rd hour post carrageenan injection. The results indicated that the plant could be a potential source of antioxidant and could find a use in the herbal therapy of inflammation also.

Despite progress made in modern medical science herbal drugs constitute a major share of all the ... more Despite progress made in modern medical science herbal drugs constitute a major share of all the officially recognized systems of health in India viz. Ayurveda, Yoga, Unani, Siddha, Homeopathy and Naturopathy. More than 70% of India's 1.1 billion populations still use these non-allopathic systems of medicine. Various synthetic drugs like Sildenafil citrate, Tadalafil citrate, Vardenafil, etc are used to treat ED but these drugs also have fatal side effects. The use of synthetic aphrodisiacs results in the dilation of blood vessels in other parts of the body causing headache and fainting. Other side effects include facial flushing, stomach upset, blurred vision and sensitivity to light which usually occur at higher doses. Thus, there is growing need to look for aphrodisiacs more of herbal origin as opposed to synthetic compounds which are known to cause severe unwanted side effects. Unlike the old-time aphrodisiacs, which were meant only to increase sex drive and/or sexual pleasu...

International Journal of Diabetes Mellitus, 2015

Journal of Acute Disease, 2013

Life sciences, Jan 15, 2018

Journal of Applied Pharmaceutical Science

International Research Journal of Pharmacy, 2015

Der Pharmacia Sinica

Salvia officinalis is a Ayurvedic traditional medicinal plant in India and is considered useful i... more Salvia officinalis is a Ayurvedic traditional medicinal plant in India and is considered useful in various ailments. Preliminary neuropsychopharmacological evaluation revealed cognition enhancing ability of the flower extract of the plant. Therefore, the present study aims at evaluating the potential effectiveness of the methanol extract of the flowers of this plant in dementia related to Alzheimer's disease (AD). Dementia was induced experimentally in animals by ingesting excess Copper and Aluminium in drinking water and confirmed by Radial 8 arm maze test. Differentl biochemical parameters including superoxide dismutase (SOD), glutathione-oxidized (GSSG), reduced glutathione (GSH), nitrite estimation, myeloperoxidase (MPO) activity and acetylcholinesterase (AChE) activity were also estimated. Lipid profile was also evaluated along with the histopathological observations. Results demonstrated a neuroprotective profile of the methanol extract of the flowers of Salvia officinalis...

INTERNATIONAL JOURNAL OF ADVANCES IN PHARMACEUTICAL RESEARCH

Despite progress made in modern medical science herbal drugs constitute a major share of all the ... more Despite progress made in modern medical science herbal drugs constitute a major share of all the officially recognized systems of health in India viz. Ayurveda, Yoga, Unani, Siddha, Homeopathy and Naturopathy. More than 70% of India's 1.1 billion populations still use these non-allopathic systems of medicine. Various synthetic drugs like Sildenafil citrate, Tadalafil citrate, Vardenafil, etc are used to treat ED but these drugs also have fatal side effects. The use of synthetic aphrodisiacs results in the dilation of blood vessels in other parts of the body causing headache and fainting. Other side effects include facial flushing, stomach upset, blurred vision and sensitivity to light which usually occur at higher doses. Thus, there is growing need to look for aphrodisiacs more of herbal origin as opposed to synthetic compounds which are known to cause severe unwanted side effects. Unlike the old-time aphrodisiacs, which were meant only to increase sex drive and/or sexual pleasu...