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Papers by xinchen teng

Research paper thumbnail of The effect of the EAAEAE insert on the property of human metallothionein-3

Protein Engineering Design & Selection, 2003

MT3 shows apparently different properties and function from MT1 even though they have 70% sequenc... more MT3 shows apparently different properties and function from MT1 even though they have 70% sequence homology. Possibly the two inserts, Thr5 and a negatively charged hexapeptide at position-55 in MT3, play important roles. A series of MT3 variants around the EAAEAE hexapeptide have been prepared by site-directed mutagenesis and their properties and reactivity towards pH, EDTA and DTNB have been studied. Our detailed studies revealed that the EAAEAE insert is essential to the property of MT3. It is the hexapeptide insert, to some extent, making the MT3 alpha-domain looser and lower stability of the metal-thiolate cluster, which could be accessed more easily.

Research paper thumbnail of Interaction of zinc finger ZNF191(243—368) and its I323 W and R327W mutants with oligonucleotides by florescence spectrum

Science China-chemistry, 2003

Two mutants of the zinc finger protein, ZNF191 (243–368) I323W and R327W, are successfully obtain... more Two mutants of the zinc finger protein, ZNF191 (243–368) I323W and R327W, are successfully obtained by site-directed mutagenesis. The fluorescence spectrum is used to study the interaction between these two mutants and the oligonucleotides. The influence of the mutation on the interaction has been studied using ethidium bromide (EB) as the fluorescence probe. The binding constants of the I323W-DNA and R327W-DNA have been calculated and the possible binding models have been discussed.

Research paper thumbnail of Fis1 deficiency selects for compensatory mutations responsible for cell death and growth control defects

Cell Death and Differentiation, 2008

Research paper thumbnail of The Apoptosome at High Resolution

Cell, 2010

The mechanism by which the apoptosome activates caspases during apoptosis has been controversial.... more The mechanism by which the apoptosome activates caspases during apoptosis has been controversial. Qi et al. (2010) now present a crystal structure of a funnel-shaped octameric apoptosome complex from the nematode Caenorhabditis elegans that challenges currently held assumptions about the human apoptosome structure.

Research paper thumbnail of Gene-dependent cell death in yeast

Cell Death and Disease, 2011

Research paper thumbnail of Mitochondrial involvement in cell death of non-mammalian eukaryotes

Biochimica Et Biophysica Acta-molecular Cell Research, 2011

Research paper thumbnail of The effect of the EAAEAE insert on the property of human metallothionein-3

Protein Engineering Design & Selection, 2003

MT3 shows apparently different properties and function from MT1 even though they have 70% sequenc... more MT3 shows apparently different properties and function from MT1 even though they have 70% sequence homology. Possibly the two inserts, Thr5 and a negatively charged hexapeptide at position-55 in MT3, play important roles. A series of MT3 variants around the EAAEAE hexapeptide have been prepared by site-directed mutagenesis and their properties and reactivity towards pH, EDTA and DTNB have been studied. Our detailed studies revealed that the EAAEAE insert is essential to the property of MT3. It is the hexapeptide insert, to some extent, making the MT3 alpha-domain looser and lower stability of the metal-thiolate cluster, which could be accessed more easily.

Research paper thumbnail of Interaction of zinc finger ZNF191(243—368) and its I323 W and R327W mutants with oligonucleotides by florescence spectrum

Science China-chemistry, 2003

Two mutants of the zinc finger protein, ZNF191 (243–368) I323W and R327W, are successfully obtain... more Two mutants of the zinc finger protein, ZNF191 (243–368) I323W and R327W, are successfully obtained by site-directed mutagenesis. The fluorescence spectrum is used to study the interaction between these two mutants and the oligonucleotides. The influence of the mutation on the interaction has been studied using ethidium bromide (EB) as the fluorescence probe. The binding constants of the I323W-DNA and R327W-DNA have been calculated and the possible binding models have been discussed.

Research paper thumbnail of Fis1 deficiency selects for compensatory mutations responsible for cell death and growth control defects

Cell Death and Differentiation, 2008

Research paper thumbnail of The Apoptosome at High Resolution

Cell, 2010

The mechanism by which the apoptosome activates caspases during apoptosis has been controversial.... more The mechanism by which the apoptosome activates caspases during apoptosis has been controversial. Qi et al. (2010) now present a crystal structure of a funnel-shaped octameric apoptosome complex from the nematode Caenorhabditis elegans that challenges currently held assumptions about the human apoptosome structure.

Research paper thumbnail of Gene-dependent cell death in yeast

Cell Death and Disease, 2011

Research paper thumbnail of Mitochondrial involvement in cell death of non-mammalian eukaryotes

Biochimica Et Biophysica Acta-molecular Cell Research, 2011

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