Md. Abul Kalam Azad | USTC (original) (raw)
Papers by Md. Abul Kalam Azad
International Journal Of Community Medicine And Public Health
Background: The first major outbreak in Bangladesh was reported in 2008 in Rajshahi and Chapainaw... more Background: The first major outbreak in Bangladesh was reported in 2008 in Rajshahi and Chapainawabgonj. It then re-emerged in 2013, 2014 and 2015 mostly in Dhaka and other parts of Bangladesh, with a notable outbreak in December 2016 according to a report. It is a statistical report on the data retrieved from chikungunya patients in the Chittagong area, specifically in relation to age, gender, location, symptoms and to assess the magnitude of the outbreak with an interest in identifying the potential socio-environmental factors which may be responsible for chikungunya in respect to Chittagong.Methods: Collection of reports from well-established diagnostic laboratories, as well as, limited survey data during July to December, 2017. There were 188 clinically suspected chikungunya cases in both genders ranging from 2-70 years of age.Results: The total chikungunya cases in the study area it has been found that the highest number of cases were found in the age group of 41-50 years (25%)...
Fresh fruits and vegetables are an essential part of people's diet all over the world. Since they... more Fresh fruits and vegetables are an essential part of people's diet all over the world. Since they are typically eaten raw and often without heat treatment or thorough washing, so they serve as vectors for the transmission of pathogenic microorganisms associated with human diseases. This study aimed at isolating and identifying the pathogenic bacteria from selected popular fresh fruits and vegetables which are known as street food. A total of 7 fresh fruits and vegetables were collected from different open markets of Chittagong with aseptic condition. Selective and non-selective media were used to isolate and enumerate the bacteria. All the species were identified on the basis of morphology, biochemical tests (IMViC) and selective differential culture media. Among the samples Vibrio spp., Lactobacillus spp., Pseudomonas spp. and Salmonella spp. were dominant. All of fresh fruits and vegetables were heavily contaminated with coliform and fecal coliform (> 1100 CFU/100ml). Range of microbial count of guava was 2.1×10 4 CFU/ml, apple was 4.3×10 4 CFU/ml, tomato was 8.2×10 4 CFU/ml, cucumber was 5.0×10 4 CFU/ml, carrot was 1.3× ×10 4 CFU/ml, hog plum (Amra) was 7.5×10 4 CFU/ml. This results indicated that the serious concern for the people of Chittagong area as it can be a great risk for human health. So this results, suggested the necessity to create awareness about hygienic practices in selling and buying fresh fruits and vegetables.
Journal of Advanced Biotechnology and Experimental Therapeutics, 2020
ABSTRACT: Accurate identification of important plants is essential for their safety, efficacy and... more ABSTRACT: Accurate identification of important plants is essential for their safety, efficacy and herbal remedies. The study was aimed to identify 4 locally available medicinal plants using DNA barcoding approach. Genomic DNA was extracted from plant samples followed by their amplification by the conventional PCR approach. Short sequence diversity of standardized specific coding gene regions of matK gene of plastid genome was used to compare and differentiate the plant species. Subsequently, all the samples were purified and sequenced successfully. A phylogenetic tree was constructed to assess their cross-species relationship. All the samples showed a high similarity rate with their homologs after blasting them in NCBI database. The phylogenetic study showed a distinguished relationship with each other. All the result indicates that DNA barcoding approach could be successfully used for reliable identification of medicinal plants and matK gene is a good candidate for this approach.
International Journal of Community Medicine and Public Health, 2019
http://dx.doi.org/10.18203/2394-6040.ijcmph20195039
International Journal Of Community Medicine And Public Health
Background: The first major outbreak in Bangladesh was reported in 2008 in Rajshahi and Chapainaw... more Background: The first major outbreak in Bangladesh was reported in 2008 in Rajshahi and Chapainawabgonj. It then re-emerged in 2013, 2014 and 2015 mostly in Dhaka and other parts of Bangladesh, with a notable outbreak in December 2016 according to a report. It is a statistical report on the data retrieved from chikungunya patients in the Chittagong area, specifically in relation to age, gender, location, symptoms and to assess the magnitude of the outbreak with an interest in identifying the potential socio-environmental factors which may be responsible for chikungunya in respect to Chittagong.Methods: Collection of reports from well-established diagnostic laboratories, as well as, limited survey data during July to December, 2017. There were 188 clinically suspected chikungunya cases in both genders ranging from 2-70 years of age.Results: The total chikungunya cases in the study area it has been found that the highest number of cases were found in the age group of 41-50 years (25%)...
Fresh fruits and vegetables are an essential part of people's diet all over the world. Since they... more Fresh fruits and vegetables are an essential part of people's diet all over the world. Since they are typically eaten raw and often without heat treatment or thorough washing, so they serve as vectors for the transmission of pathogenic microorganisms associated with human diseases. This study aimed at isolating and identifying the pathogenic bacteria from selected popular fresh fruits and vegetables which are known as street food. A total of 7 fresh fruits and vegetables were collected from different open markets of Chittagong with aseptic condition. Selective and non-selective media were used to isolate and enumerate the bacteria. All the species were identified on the basis of morphology, biochemical tests (IMViC) and selective differential culture media. Among the samples Vibrio spp., Lactobacillus spp., Pseudomonas spp. and Salmonella spp. were dominant. All of fresh fruits and vegetables were heavily contaminated with coliform and fecal coliform (> 1100 CFU/100ml). Range of microbial count of guava was 2.1×10 4 CFU/ml, apple was 4.3×10 4 CFU/ml, tomato was 8.2×10 4 CFU/ml, cucumber was 5.0×10 4 CFU/ml, carrot was 1.3× ×10 4 CFU/ml, hog plum (Amra) was 7.5×10 4 CFU/ml. This results indicated that the serious concern for the people of Chittagong area as it can be a great risk for human health. So this results, suggested the necessity to create awareness about hygienic practices in selling and buying fresh fruits and vegetables.
Journal of Advanced Biotechnology and Experimental Therapeutics, 2020
ABSTRACT: Accurate identification of important plants is essential for their safety, efficacy and... more ABSTRACT: Accurate identification of important plants is essential for their safety, efficacy and herbal remedies. The study was aimed to identify 4 locally available medicinal plants using DNA barcoding approach. Genomic DNA was extracted from plant samples followed by their amplification by the conventional PCR approach. Short sequence diversity of standardized specific coding gene regions of matK gene of plastid genome was used to compare and differentiate the plant species. Subsequently, all the samples were purified and sequenced successfully. A phylogenetic tree was constructed to assess their cross-species relationship. All the samples showed a high similarity rate with their homologs after blasting them in NCBI database. The phylogenetic study showed a distinguished relationship with each other. All the result indicates that DNA barcoding approach could be successfully used for reliable identification of medicinal plants and matK gene is a good candidate for this approach.
International Journal of Community Medicine and Public Health, 2019