Khumukcham Nongalleima | Institute of Bioresources and Sustainable Development (IBSD), Dept. of Biotechnology, Govt. of India, Takyelpat Institutional Area, Imphal. India (original) (raw)
Papers by Khumukcham Nongalleima
Journal of Pharmacognosy and Phytochemistry, 2017
Citrus macroptera Montruz. fruits were collected, its rind was dried and powdered. Three differen... more Citrus macroptera Montruz. fruits were collected, its rind was dried and powdered. Three different organic extracts, aqueous (NC1), methanol (NC2) and aqueous methanol (NC3) were prepared. In vitro antioxidant and reverse phase High Performance Liquid Chromatography (RP-HPLC) was done to quantify the quercetin, rutin and kaempferol content in three extracts of Citrus macroptera Montruz. NC1 showed highest antioxidant (DPPH-IC50 87.83 μg/mg, reducing power assay 36.71 μg/mg). Quercetin and kaempferol content was highest in NC2 extract, was quantified as 431.1 μg/mL and 59.50 μg/mL respectively. However rutin content was highest in aqueous methanol extract (260.38 μg/mL). The designed studies indicate remarkable potential of C. macroptera as dietary source of antioxidant and also the presence of important flavonoids. Studies on isolation of lead compound attributing the studied bioactivity is under progress.
African Journal of Biotechnology, 2013
Complete randomized block design was applied to evaluate and compare the growth, yield and field ... more Complete randomized block design was applied to evaluate and compare the growth, yield and field performance of in vitro derived turmeric plants with conventional rhizome under field condition. In vitro propagated plants manifest consistently superior horticultural performance over the conventional rhizome. Among the different lines of in vitro propagated plants, plantlets treated with silver nitrate (AgNO3) were studied and compared with the conventional rhizome which showed superior growth in almost all the different traits compared. Phenotypic variation was higher in in vitro (3.3%) than conventional plant (1.2%) with no statistically significance. Tissue culture plants grew vigorously and taller than conventional type after six months of propagation. The highest yield potential were observed in in vitro plants (13.96 ton/ha) as compared with the conventional rhizome (6.97 ton/ha). However, the agronomic traits observed during the present study in tissue culture plants are stable...
International Journal of Pharmacy and Pharmaceutical Sciences, 2017
Objective: The present investigation was designed for Gas Chromatography Mass Spectrometry (GCMS)... more Objective: The present investigation was designed for Gas Chromatography Mass Spectrometry (GCMS) based metabolite profiling of Citrus macroptera Montruz. Leaves and peel oils followed by assessment of in vitro antioxidant and anti-inflammatory activity.Methods: Essential oil was extracted from leaves and peels of Citrus macroptera Montruz. The oil samples were subjected to GCMS analysis using Shimadzu GCMS-QP2010 equiped with an AOC-2oi auto-injector and AOC-2os autosampler units. In vitro antioxidant activities were evaluated using DPPH radical scavenging, reducing power and nitric oxide reducing method. In vitro anti-inflammatory activity was evaluated using protease inhibitory assay, heat induced haemolysis and albumin denaturation assay.Results: Both the peels and leaves of Citrus macroptera Montruz. Yielded good amount of essential oil. 57 compounds each were identified from leaves as well as peel of C. macroptera. 10 common compounds have been detected in both the oil samples...
Current World Environment, 2014
Among the rice varieties, Purple Rice has an incredibly rich history and counts among its strains... more Among the rice varieties, Purple Rice has an incredibly rich history and counts among its strains one variety known as Imperial rice as they are reserved for the Emperor's consumption only. The purple color is due to high anthocyanin content, a trait most typically observed in fruits. There are abundant benefits including medicinal properties of rice. Its medicinal properties being imparted by the phytochemicals present in it, some of the important compounds which are worth mentioning are tocotrienols, gamma amino butyric acid, oryzanol, rice bran saccharide, Lutein, Zeaxanthin, butylate hydroanisole, phytosterol. Chakhao, the aromatic black glutinous rice in Manipur, India has an aesthetic value and nutraceutical properties. There is a huge demand in the domestic market, having possibilities for export, thus, it is a need for the improvement of these cultivars for higher production, productivity without losing grain quality characteristics, and its applications in pharmaceutical sciences. In this review, we focus on the nutritional values of rice with nutritional properties and their impact on human health.
International Journal of PharmTech Research
An endophytic fungus was isolated from rhizomes of Zingiber zerumbet (L.) Sm. (Z.z) an important ... more An endophytic fungus was isolated from rhizomes of Zingiber zerumbet (L.) Sm. (Z.z) an important medicinal plant of North-East India. Colonial morphological trait, microscopic observation and molecular sequence analysis of ITS region of isolated fungus insinuated 99% similarity with Fusarium oxysporum. The isolated and identified fungus was then cultivated in Potato Dextrose Broth for 25 days at 25±1 0 C. Broth and mycelia were separated, fungal broth was extracted with different solvent system; methanol (Zfe 1), n-butanol (Zfe 2), hexane (Zfe 3), and mycelia was extracted with ethyl acetate (Zfe 4). The extracts (Zfe 1, Zfe 2, Zfe 3, Zfe 4) were subjected to screening and estimation of DPPH free radical scavenging activity, total phenolics content, total flavonoid content, cyclo-oxygenase inhibition assay. Among the tested extract, Zfe 3 shows highest DPPH radical scavenging activity and COX II inhibition with IC 50 value of 41.68 µg and 14.27 µg/100 mg respectively. However, pheno...
Journal of Ethnopharmacology, 2014
Ethnopharmacological relevance: Plant based traditional medicines for birth control has been in p... more Ethnopharmacological relevance: Plant based traditional medicines for birth control has been in practice in rural populations of NorthEast India, since time immemorial. Fresh rhizome of Drynaria quercifolia (L.) J. Smith is one of the plant parts used traditionally by different ethnic communities in Tripura, India for birth control. The present investigation is aimed to justify the scientific basis in traditional use of Drynaria quercifolia rhizome as anti-fertility agent. Materials and methods: Ex-vivo uterotonic activity was done on uterine tissue. Acute toxicity test of the plant extract was carried out in rats of both sexes. The abortifacient and anti-implantation activities of the extract were investigated, in-vivo and estimated the level of different hormones release. Results: The result revealed the effectiveness of methanol (87%) and aqueous (68%) extract of the plant on uterotonic activity. The extracts showed relatively non-toxic effect in acute toxicity study. Methanolic extract has shown higher efficacy for both abortifacient (nn po 0.01) and anti-implantation performance (nn po 0.01) and also effected hormone release level (nn po 0.01). Conclusion: Methanolic extract of Drynaria quercifolia (L.) J. Smith rhizome has been proved to have significant anti-fertility activity.
International Journal of PharmTech Research, 2017
Citrus macroptera Montruz. fruits were collected, its rind was dried and powdered. Three differen... more Citrus macroptera Montruz. fruits were collected, its rind was dried and powdered. Three different organic extracts, aqueous (NC1), methanol (NC2) and aqueous methanol (NC3) were prepared. In vitro antioxidant and anti-inflammatory activities were done followed by in vivo studies. NC1 showed highest antioxidant (DPPH-IC 50 87.83 µg/g, reducing power assay 36.71 µg/g) and highest protease inhibitory (20.44 µg/mL) activity. It showed IC 50 148.44 µg/mL against HeLa cells in MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5diphnyltetrazolium bromide) assay. In in vivo anti-inflammatory activity assay, the effect was almost comparable with standard drug diclofenac sodium at 4 th and 6 th hr after administration when compared to the control group. NC2 exhibited more amount of anti-inflammatory activity than the NC1 and NC3 extract. The designed studies indicate remarkable potential of C. macroptera as dietary source of antioxidant, anti-inflammatory and cytotoxic agents. Studies on isolation of lead compound attributing the studied bioactivity is under progress.
Among the rice varieties, Purple Rice has an incredibly rich history and counts among its strains... more Among the rice varieties, Purple Rice has an incredibly rich history and counts among its strains one variety known as Imperial rice as they are reserved for the Emperor’s consumption only. The purple color is due to high anthocyanin content, a trait most typically observed in fruits. There are abundant benefits including medicinal properties of rice. Its medicinal properties being imparted by the phytochemicals present in it, some of the important compounds which are worth mentioning are tocotrienols, gamma amino butyric acid, oryzanol, rice bran saccharide, Lutein, Zeaxanthin, butylate hydroanisole, phytosterol. Chakhao, the aromatic black glutinous rice in Manipur, India has an aesthetic value and nutraceutical properties. There is a huge demand in the domestic market, having possibilities for export, thus, it is a need for the improvement of these cultivars for higher production, productivity without losing grain quality characteristics, and its applications in pharmaceutical sc...
Current Bioactive Compounds, 2020
Background: Quercus serrata Murray leaves are traditionally used for the treatment of diabetes, d... more Background: Quercus serrata Murray leaves are traditionally used for the treatment of diabetes, dysmenorrhoea, inflammation and urinary tract infection. So, far no study has reported on the toxicological profile and antioxidant properties of the plant. Objective: The present study aimed to investigate the in-vivo toxicological profile and in-vitro antioxidant activities of the methanolic extract of standardized Quercus serrata leaves. Methods: Per-oral sub-acute toxicity study was performed in rats using three dose levels (200, 400 and 800 mg/kg b.w.) of the extract for 28 days. The control group received gum acacia suspended in water. Bodyweight was measured weekly. Biochemical parameters were analysed using the serum; the blood-cell count was performed using the whole blood. Pathological changes were also checked in highly perfused tissues. Further, in-vitro reducing power assay, nitric oxide scavenging assay, and DPPH free-radical scavenging assay were performed to evaluate the a...
Scientific reports, Jan 6, 2018
Zerumbone is a known anti-cancer herbal compound. However, the actual protein target is not fully... more Zerumbone is a known anti-cancer herbal compound. However, the actual protein target is not fully understood or known. This investigation focus on the association of zerumbone in HCT116 colon cancer cell proliferation and its link with TNF-alpha. The study shows that with the increasing concentration of zerumbone, there was a reduction of HCT116 cells proliferation based on the cell line study and hence higher TNF-alpha inhibition based on the TNF-alpha assay. The study also emphasizes on the computational aspect by investigating the molecular docking analysis of zerumbone against TNF-alpha. The docked complex was further validated using molecular dynamics simulation studies. The docking analysis observed that alpha-beta unsaturated carbonyl scaffold is an important moiety for the anti-cancer activity of zerumbone. Furthermore, the DFT analysis also confirms the reactivity nature of zerumbone based on the frontier molecular orbital analysis.
Chemo-Biological Systems for CO2 Utilization, 2020
Letters in Drug Design & Discovery, 2021
Background: There are several reports on the anti-cancer property of zerumbone, such as in breast... more Background: There are several reports on the anti-cancer property of zerumbone, such as in breast, cervical and ovarian cancer. But the investigation of the actual protein target has been the least concerned and there are few reports on the inhibitory effect of zerumbone against specific cancer-causing proteins and enzymes. Therefore, further investigation is required at a much deeper molecular level. Objective: This study aimed to determine the anti-proliferative activity of Zerumbone against cervical cancer cell and assess its TNF-α enzyme inhibitory action. Methods: The investigation emphasized the anti-cancer activity of zerumbone against HeLa cells and its subsequent TNF-α assay. Furthermore, computational studies on Zerumbone as an inhibitor of TNF-α were carried out using computational techniques such as docking and MD simulations. Results and Discussion: From the molecular docking analysis, it was observed and substantiated that the α,β-Unsaturated carbonyl scaffold is the m...
Medicinal Chemistry Research, 2019
A small molecule library of trans-1,3-diaryl-1H-naphtho[1,2-e][1,3]oxazines 4 is synthesised thro... more A small molecule library of trans-1,3-diaryl-1H-naphtho[1,2-e][1,3]oxazines 4 is synthesised through multicomponent reactions involving aliphatic amines, aromatic aldehydes and β-naphthol using a heterogeneous catalyst, SiO2.HClO4, and ethanol as the solvent. The anti-inflammatory activities of the synthesised compounds are evaluated together with in silico studies. 1,3-Bis(4-chlorophenyl)-2-ethyl-2,3-dihydro-1H-naphtho[1,2-e][1,3]oxazine (4h) shows the best activity with IC50 = 4.807 µg/mL in the heat-induced haemolysis, while 1,3-Bis(5-bromothiophen-2-yl)-2-ethyl-2,3-dihydro-1H-naphtho[1,2-e][1,3]oxazine (4c) shows significantly high anti-inflammatory activity (IC50 = 5.5 µg/mL) in comparison with standard drugs, such as sodium diclofenac. In addition, molecular docking simulation study confirms the in-depth molecular interaction of the two lead compounds, 4c and 4h with better binding affinities and docking scores at the active site of the COX-2 enzyme compared with diclofenac. SiO2.HClO4 as a heterogeneous catalyst in the synthesis of naphtho[1,2-e][1,3]oxazine. The anti-inflammatory activities of the synthesised compounds were also analysed along with the in silico studies. The importance of naphtho[1,2-e][1,3]oxazine are highlighted. The catalyst is easily accessible and reusable. SiO2.HClO4 as a heterogeneous catalyst in the synthesis of naphtho[1,2-e][1,3]oxazine. The anti-inflammatory activities of the synthesised compounds were also analysed along with the in silico studies. The importance of naphtho[1,2-e][1,3]oxazine are highlighted. The catalyst is easily accessible and reusable.
Biocatalysis and Agricultural Biotechnology, 2020
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Network Modeling Analysis in Health Informatics and Bioinformatics, 2018
The inhibitor of kappaB kinase beta (IKK-β) is an important target for the therapeutic treatment ... more The inhibitor of kappaB kinase beta (IKK-β) is an important target for the therapeutic treatment of cancer and inflammatory diseases. On the other hand, zerumbone an isolate from Zingiber zerumbet has become an important molecule for targeting various cancer drug targets and enzyme. Although zerumbone is also a good inhibitor of IKK-β, its usage is limited because of its bioavailability problem. Hence, the objective of this investigation focuses on discovering new analogs of zerumbone with better bioavailability. The study involves DFT-based molecular docking and molecular dynamics simulation studies on zerumbone against the IKK-β enzyme. The best docking pose of the analogs was further analyzed for molecular interaction map and carried forward for molecular dynamics simulation production. They were also analyzed for HOMO and LUMO energies calculated at DFT/B3LYP/6-31G (d′, p′) levels of theory to calculate the band gap energy. The study observed that the analogs of zerumbone inhibit the IKK-β with favorable binding affinity than zerumbone.
Isozyme studies provide preliminary indications that genetic variation may be differently apporti... more Isozyme studies provide preliminary indications that genetic variation may be differently apportioned in some crops compared to their wild relatives. The present investigation was undertaken to study the Isozyme banding patterns and its inheritance in rice with an aim to establish the genetic diversity among the 9 cultivated and wild rice species of Manipur. The indigenous rice collections were subjected to analysis of Isozyme polymorphism at three loci by Polyacrylamide gel electrophoresis. Zymograms of all the tested enzyme system (esterase, alcohol dehydrogenase, glutamate dehydrogenase) were then constructed, genetic relationships between the nine varieties on the number of loci at which Isozyme pattern differs were determined. In all the enzyme systems studied, only one zone of isozymatic activity defined by the degree of mobility could be observed. Maximum number of bands was observed in Taothabi and Wainuchara (O. rufipogon) local collection I in comparison to other germplasm...
Journal of ethnopharmacology, Jan 17, 2015
Traditional health care practices are still being followed extensively in Manipur, North East Ind... more Traditional health care practices are still being followed extensively in Manipur, North East India. This is the major or the only medical facility available in some rural areas of Manipur. Cross cultural ethno-pharmacological survey was conducted to document traditional health care practices by Maiba-Maibi (male-female traditional health care practitioners of Manipur). All together 59 traditional practitioners belonging to 12 ethnic communities in nine districts of the Manipur state was interviewed. A predesigned questionnaire was used for interviews, which included queries for type of ailments treating, symptoms, bioresources used, method of preparation, dosage forms, formulation, unit doses. The entire interviews done in the residence of respective Maiba-Maibi, their patient handing and preparation of medicinal formulations were documented in written and audio-visual format. The survey recorded traditional knowledge on 949 formulations used for 66 human ailments. Five hundred for...
Phytochemistry Reviews, 2011
Numerous researches have been carried out in Zingiber zerumbet Smith. Since 1944 till date. Z. ze... more Numerous researches have been carried out in Zingiber zerumbet Smith. Since 1944 till date. Z. zerumbet is a monocotyledonous perennial medicinal plant belonging to Zingiberaceae family. It is commonly known as shampoo ginger. It has many different local names depending on their area of collection and vegetation. It is called as 'Singkha' in Manipuri. Various compounds have been reported to be isolated from Z. zerumbet and they serve a very potent and reliable drug candidate for the various diseases. They have been investigated for its prospects of effectiveness against number of activities in in vitro as well as in vivo and mechanisms that may be involved in chemo preventive measures and various pharmaceutical studies.
Food & function, 2014
An emulsified gel of (+)-catechin was developed and evaluated topically against 7,12-dimethylbenz... more An emulsified gel of (+)-catechin was developed and evaluated topically against 7,12-dimethylbenz(a)anthracene-induced and 12-O-tetradecanoylphorbol-13-acetate-promoted (DMBA-induced and TPA-promoted) squamous cell carcinoma of the skin in BALB/c mice. The biological evaluation outcome indicated that the (+)-catechin emulsified gel increased the activity of oxidative stress biomarkers glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), glutathione reductase (GR), and glutathione peroxidase (GPx), whereas it decreased the level of malondialdehyde (MDA). The mechanistic study showed that genes implicated in the inflammation and cancer, such as cyclooxygenase-2 (COX-2), nuclear factor-kappa B (NF-κB), and inducible nitric-oxide synthase (iNOS), were down-regulated by (+)-catechin emulsified gel while inhibiting an inflammatory mediator prostaglandin E2 (PGE2). The (+)-catechin emulsified gel further suppressed the activity of pro-inflammatory...
Journal of Pharmacognosy and Phytochemistry, 2017
Citrus macroptera Montruz. fruits were collected, its rind was dried and powdered. Three differen... more Citrus macroptera Montruz. fruits were collected, its rind was dried and powdered. Three different organic extracts, aqueous (NC1), methanol (NC2) and aqueous methanol (NC3) were prepared. In vitro antioxidant and reverse phase High Performance Liquid Chromatography (RP-HPLC) was done to quantify the quercetin, rutin and kaempferol content in three extracts of Citrus macroptera Montruz. NC1 showed highest antioxidant (DPPH-IC50 87.83 μg/mg, reducing power assay 36.71 μg/mg). Quercetin and kaempferol content was highest in NC2 extract, was quantified as 431.1 μg/mL and 59.50 μg/mL respectively. However rutin content was highest in aqueous methanol extract (260.38 μg/mL). The designed studies indicate remarkable potential of C. macroptera as dietary source of antioxidant and also the presence of important flavonoids. Studies on isolation of lead compound attributing the studied bioactivity is under progress.
African Journal of Biotechnology, 2013
Complete randomized block design was applied to evaluate and compare the growth, yield and field ... more Complete randomized block design was applied to evaluate and compare the growth, yield and field performance of in vitro derived turmeric plants with conventional rhizome under field condition. In vitro propagated plants manifest consistently superior horticultural performance over the conventional rhizome. Among the different lines of in vitro propagated plants, plantlets treated with silver nitrate (AgNO3) were studied and compared with the conventional rhizome which showed superior growth in almost all the different traits compared. Phenotypic variation was higher in in vitro (3.3%) than conventional plant (1.2%) with no statistically significance. Tissue culture plants grew vigorously and taller than conventional type after six months of propagation. The highest yield potential were observed in in vitro plants (13.96 ton/ha) as compared with the conventional rhizome (6.97 ton/ha). However, the agronomic traits observed during the present study in tissue culture plants are stable...
International Journal of Pharmacy and Pharmaceutical Sciences, 2017
Objective: The present investigation was designed for Gas Chromatography Mass Spectrometry (GCMS)... more Objective: The present investigation was designed for Gas Chromatography Mass Spectrometry (GCMS) based metabolite profiling of Citrus macroptera Montruz. Leaves and peel oils followed by assessment of in vitro antioxidant and anti-inflammatory activity.Methods: Essential oil was extracted from leaves and peels of Citrus macroptera Montruz. The oil samples were subjected to GCMS analysis using Shimadzu GCMS-QP2010 equiped with an AOC-2oi auto-injector and AOC-2os autosampler units. In vitro antioxidant activities were evaluated using DPPH radical scavenging, reducing power and nitric oxide reducing method. In vitro anti-inflammatory activity was evaluated using protease inhibitory assay, heat induced haemolysis and albumin denaturation assay.Results: Both the peels and leaves of Citrus macroptera Montruz. Yielded good amount of essential oil. 57 compounds each were identified from leaves as well as peel of C. macroptera. 10 common compounds have been detected in both the oil samples...
Current World Environment, 2014
Among the rice varieties, Purple Rice has an incredibly rich history and counts among its strains... more Among the rice varieties, Purple Rice has an incredibly rich history and counts among its strains one variety known as Imperial rice as they are reserved for the Emperor's consumption only. The purple color is due to high anthocyanin content, a trait most typically observed in fruits. There are abundant benefits including medicinal properties of rice. Its medicinal properties being imparted by the phytochemicals present in it, some of the important compounds which are worth mentioning are tocotrienols, gamma amino butyric acid, oryzanol, rice bran saccharide, Lutein, Zeaxanthin, butylate hydroanisole, phytosterol. Chakhao, the aromatic black glutinous rice in Manipur, India has an aesthetic value and nutraceutical properties. There is a huge demand in the domestic market, having possibilities for export, thus, it is a need for the improvement of these cultivars for higher production, productivity without losing grain quality characteristics, and its applications in pharmaceutical sciences. In this review, we focus on the nutritional values of rice with nutritional properties and their impact on human health.
International Journal of PharmTech Research
An endophytic fungus was isolated from rhizomes of Zingiber zerumbet (L.) Sm. (Z.z) an important ... more An endophytic fungus was isolated from rhizomes of Zingiber zerumbet (L.) Sm. (Z.z) an important medicinal plant of North-East India. Colonial morphological trait, microscopic observation and molecular sequence analysis of ITS region of isolated fungus insinuated 99% similarity with Fusarium oxysporum. The isolated and identified fungus was then cultivated in Potato Dextrose Broth for 25 days at 25±1 0 C. Broth and mycelia were separated, fungal broth was extracted with different solvent system; methanol (Zfe 1), n-butanol (Zfe 2), hexane (Zfe 3), and mycelia was extracted with ethyl acetate (Zfe 4). The extracts (Zfe 1, Zfe 2, Zfe 3, Zfe 4) were subjected to screening and estimation of DPPH free radical scavenging activity, total phenolics content, total flavonoid content, cyclo-oxygenase inhibition assay. Among the tested extract, Zfe 3 shows highest DPPH radical scavenging activity and COX II inhibition with IC 50 value of 41.68 µg and 14.27 µg/100 mg respectively. However, pheno...
Journal of Ethnopharmacology, 2014
Ethnopharmacological relevance: Plant based traditional medicines for birth control has been in p... more Ethnopharmacological relevance: Plant based traditional medicines for birth control has been in practice in rural populations of NorthEast India, since time immemorial. Fresh rhizome of Drynaria quercifolia (L.) J. Smith is one of the plant parts used traditionally by different ethnic communities in Tripura, India for birth control. The present investigation is aimed to justify the scientific basis in traditional use of Drynaria quercifolia rhizome as anti-fertility agent. Materials and methods: Ex-vivo uterotonic activity was done on uterine tissue. Acute toxicity test of the plant extract was carried out in rats of both sexes. The abortifacient and anti-implantation activities of the extract were investigated, in-vivo and estimated the level of different hormones release. Results: The result revealed the effectiveness of methanol (87%) and aqueous (68%) extract of the plant on uterotonic activity. The extracts showed relatively non-toxic effect in acute toxicity study. Methanolic extract has shown higher efficacy for both abortifacient (nn po 0.01) and anti-implantation performance (nn po 0.01) and also effected hormone release level (nn po 0.01). Conclusion: Methanolic extract of Drynaria quercifolia (L.) J. Smith rhizome has been proved to have significant anti-fertility activity.
International Journal of PharmTech Research, 2017
Citrus macroptera Montruz. fruits were collected, its rind was dried and powdered. Three differen... more Citrus macroptera Montruz. fruits were collected, its rind was dried and powdered. Three different organic extracts, aqueous (NC1), methanol (NC2) and aqueous methanol (NC3) were prepared. In vitro antioxidant and anti-inflammatory activities were done followed by in vivo studies. NC1 showed highest antioxidant (DPPH-IC 50 87.83 µg/g, reducing power assay 36.71 µg/g) and highest protease inhibitory (20.44 µg/mL) activity. It showed IC 50 148.44 µg/mL against HeLa cells in MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5diphnyltetrazolium bromide) assay. In in vivo anti-inflammatory activity assay, the effect was almost comparable with standard drug diclofenac sodium at 4 th and 6 th hr after administration when compared to the control group. NC2 exhibited more amount of anti-inflammatory activity than the NC1 and NC3 extract. The designed studies indicate remarkable potential of C. macroptera as dietary source of antioxidant, anti-inflammatory and cytotoxic agents. Studies on isolation of lead compound attributing the studied bioactivity is under progress.
Among the rice varieties, Purple Rice has an incredibly rich history and counts among its strains... more Among the rice varieties, Purple Rice has an incredibly rich history and counts among its strains one variety known as Imperial rice as they are reserved for the Emperor’s consumption only. The purple color is due to high anthocyanin content, a trait most typically observed in fruits. There are abundant benefits including medicinal properties of rice. Its medicinal properties being imparted by the phytochemicals present in it, some of the important compounds which are worth mentioning are tocotrienols, gamma amino butyric acid, oryzanol, rice bran saccharide, Lutein, Zeaxanthin, butylate hydroanisole, phytosterol. Chakhao, the aromatic black glutinous rice in Manipur, India has an aesthetic value and nutraceutical properties. There is a huge demand in the domestic market, having possibilities for export, thus, it is a need for the improvement of these cultivars for higher production, productivity without losing grain quality characteristics, and its applications in pharmaceutical sc...
Current Bioactive Compounds, 2020
Background: Quercus serrata Murray leaves are traditionally used for the treatment of diabetes, d... more Background: Quercus serrata Murray leaves are traditionally used for the treatment of diabetes, dysmenorrhoea, inflammation and urinary tract infection. So, far no study has reported on the toxicological profile and antioxidant properties of the plant. Objective: The present study aimed to investigate the in-vivo toxicological profile and in-vitro antioxidant activities of the methanolic extract of standardized Quercus serrata leaves. Methods: Per-oral sub-acute toxicity study was performed in rats using three dose levels (200, 400 and 800 mg/kg b.w.) of the extract for 28 days. The control group received gum acacia suspended in water. Bodyweight was measured weekly. Biochemical parameters were analysed using the serum; the blood-cell count was performed using the whole blood. Pathological changes were also checked in highly perfused tissues. Further, in-vitro reducing power assay, nitric oxide scavenging assay, and DPPH free-radical scavenging assay were performed to evaluate the a...
Scientific reports, Jan 6, 2018
Zerumbone is a known anti-cancer herbal compound. However, the actual protein target is not fully... more Zerumbone is a known anti-cancer herbal compound. However, the actual protein target is not fully understood or known. This investigation focus on the association of zerumbone in HCT116 colon cancer cell proliferation and its link with TNF-alpha. The study shows that with the increasing concentration of zerumbone, there was a reduction of HCT116 cells proliferation based on the cell line study and hence higher TNF-alpha inhibition based on the TNF-alpha assay. The study also emphasizes on the computational aspect by investigating the molecular docking analysis of zerumbone against TNF-alpha. The docked complex was further validated using molecular dynamics simulation studies. The docking analysis observed that alpha-beta unsaturated carbonyl scaffold is an important moiety for the anti-cancer activity of zerumbone. Furthermore, the DFT analysis also confirms the reactivity nature of zerumbone based on the frontier molecular orbital analysis.
Chemo-Biological Systems for CO2 Utilization, 2020
Letters in Drug Design & Discovery, 2021
Background: There are several reports on the anti-cancer property of zerumbone, such as in breast... more Background: There are several reports on the anti-cancer property of zerumbone, such as in breast, cervical and ovarian cancer. But the investigation of the actual protein target has been the least concerned and there are few reports on the inhibitory effect of zerumbone against specific cancer-causing proteins and enzymes. Therefore, further investigation is required at a much deeper molecular level. Objective: This study aimed to determine the anti-proliferative activity of Zerumbone against cervical cancer cell and assess its TNF-α enzyme inhibitory action. Methods: The investigation emphasized the anti-cancer activity of zerumbone against HeLa cells and its subsequent TNF-α assay. Furthermore, computational studies on Zerumbone as an inhibitor of TNF-α were carried out using computational techniques such as docking and MD simulations. Results and Discussion: From the molecular docking analysis, it was observed and substantiated that the α,β-Unsaturated carbonyl scaffold is the m...
Medicinal Chemistry Research, 2019
A small molecule library of trans-1,3-diaryl-1H-naphtho[1,2-e][1,3]oxazines 4 is synthesised thro... more A small molecule library of trans-1,3-diaryl-1H-naphtho[1,2-e][1,3]oxazines 4 is synthesised through multicomponent reactions involving aliphatic amines, aromatic aldehydes and β-naphthol using a heterogeneous catalyst, SiO2.HClO4, and ethanol as the solvent. The anti-inflammatory activities of the synthesised compounds are evaluated together with in silico studies. 1,3-Bis(4-chlorophenyl)-2-ethyl-2,3-dihydro-1H-naphtho[1,2-e][1,3]oxazine (4h) shows the best activity with IC50 = 4.807 µg/mL in the heat-induced haemolysis, while 1,3-Bis(5-bromothiophen-2-yl)-2-ethyl-2,3-dihydro-1H-naphtho[1,2-e][1,3]oxazine (4c) shows significantly high anti-inflammatory activity (IC50 = 5.5 µg/mL) in comparison with standard drugs, such as sodium diclofenac. In addition, molecular docking simulation study confirms the in-depth molecular interaction of the two lead compounds, 4c and 4h with better binding affinities and docking scores at the active site of the COX-2 enzyme compared with diclofenac. SiO2.HClO4 as a heterogeneous catalyst in the synthesis of naphtho[1,2-e][1,3]oxazine. The anti-inflammatory activities of the synthesised compounds were also analysed along with the in silico studies. The importance of naphtho[1,2-e][1,3]oxazine are highlighted. The catalyst is easily accessible and reusable. SiO2.HClO4 as a heterogeneous catalyst in the synthesis of naphtho[1,2-e][1,3]oxazine. The anti-inflammatory activities of the synthesised compounds were also analysed along with the in silico studies. The importance of naphtho[1,2-e][1,3]oxazine are highlighted. The catalyst is easily accessible and reusable.
Biocatalysis and Agricultural Biotechnology, 2020
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Network Modeling Analysis in Health Informatics and Bioinformatics, 2018
The inhibitor of kappaB kinase beta (IKK-β) is an important target for the therapeutic treatment ... more The inhibitor of kappaB kinase beta (IKK-β) is an important target for the therapeutic treatment of cancer and inflammatory diseases. On the other hand, zerumbone an isolate from Zingiber zerumbet has become an important molecule for targeting various cancer drug targets and enzyme. Although zerumbone is also a good inhibitor of IKK-β, its usage is limited because of its bioavailability problem. Hence, the objective of this investigation focuses on discovering new analogs of zerumbone with better bioavailability. The study involves DFT-based molecular docking and molecular dynamics simulation studies on zerumbone against the IKK-β enzyme. The best docking pose of the analogs was further analyzed for molecular interaction map and carried forward for molecular dynamics simulation production. They were also analyzed for HOMO and LUMO energies calculated at DFT/B3LYP/6-31G (d′, p′) levels of theory to calculate the band gap energy. The study observed that the analogs of zerumbone inhibit the IKK-β with favorable binding affinity than zerumbone.
Isozyme studies provide preliminary indications that genetic variation may be differently apporti... more Isozyme studies provide preliminary indications that genetic variation may be differently apportioned in some crops compared to their wild relatives. The present investigation was undertaken to study the Isozyme banding patterns and its inheritance in rice with an aim to establish the genetic diversity among the 9 cultivated and wild rice species of Manipur. The indigenous rice collections were subjected to analysis of Isozyme polymorphism at three loci by Polyacrylamide gel electrophoresis. Zymograms of all the tested enzyme system (esterase, alcohol dehydrogenase, glutamate dehydrogenase) were then constructed, genetic relationships between the nine varieties on the number of loci at which Isozyme pattern differs were determined. In all the enzyme systems studied, only one zone of isozymatic activity defined by the degree of mobility could be observed. Maximum number of bands was observed in Taothabi and Wainuchara (O. rufipogon) local collection I in comparison to other germplasm...
Journal of ethnopharmacology, Jan 17, 2015
Traditional health care practices are still being followed extensively in Manipur, North East Ind... more Traditional health care practices are still being followed extensively in Manipur, North East India. This is the major or the only medical facility available in some rural areas of Manipur. Cross cultural ethno-pharmacological survey was conducted to document traditional health care practices by Maiba-Maibi (male-female traditional health care practitioners of Manipur). All together 59 traditional practitioners belonging to 12 ethnic communities in nine districts of the Manipur state was interviewed. A predesigned questionnaire was used for interviews, which included queries for type of ailments treating, symptoms, bioresources used, method of preparation, dosage forms, formulation, unit doses. The entire interviews done in the residence of respective Maiba-Maibi, their patient handing and preparation of medicinal formulations were documented in written and audio-visual format. The survey recorded traditional knowledge on 949 formulations used for 66 human ailments. Five hundred for...
Phytochemistry Reviews, 2011
Numerous researches have been carried out in Zingiber zerumbet Smith. Since 1944 till date. Z. ze... more Numerous researches have been carried out in Zingiber zerumbet Smith. Since 1944 till date. Z. zerumbet is a monocotyledonous perennial medicinal plant belonging to Zingiberaceae family. It is commonly known as shampoo ginger. It has many different local names depending on their area of collection and vegetation. It is called as 'Singkha' in Manipuri. Various compounds have been reported to be isolated from Z. zerumbet and they serve a very potent and reliable drug candidate for the various diseases. They have been investigated for its prospects of effectiveness against number of activities in in vitro as well as in vivo and mechanisms that may be involved in chemo preventive measures and various pharmaceutical studies.
Food & function, 2014
An emulsified gel of (+)-catechin was developed and evaluated topically against 7,12-dimethylbenz... more An emulsified gel of (+)-catechin was developed and evaluated topically against 7,12-dimethylbenz(a)anthracene-induced and 12-O-tetradecanoylphorbol-13-acetate-promoted (DMBA-induced and TPA-promoted) squamous cell carcinoma of the skin in BALB/c mice. The biological evaluation outcome indicated that the (+)-catechin emulsified gel increased the activity of oxidative stress biomarkers glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), glutathione reductase (GR), and glutathione peroxidase (GPx), whereas it decreased the level of malondialdehyde (MDA). The mechanistic study showed that genes implicated in the inflammation and cancer, such as cyclooxygenase-2 (COX-2), nuclear factor-kappa B (NF-κB), and inducible nitric-oxide synthase (iNOS), were down-regulated by (+)-catechin emulsified gel while inhibiting an inflammatory mediator prostaglandin E2 (PGE2). The (+)-catechin emulsified gel further suppressed the activity of pro-inflammatory...