Dr Ashwani Tapwal | FRI Dehradun (original) (raw)

Papers by Dr Ashwani Tapwal

International microbiology, Jul 1, 2024

Asian Journal of Natural Product Biochemistry, Aug 13, 2023

Journal of Basic Microbiology, Dec 30, 2023

Sustainability, Oct 8, 2023

Asian journal of forestry, Jun 30, 2023

Biomaterials and polymers horizon, Dec 14, 2022

Archives of Phytopathology and Plant Protection

The impact of hazard by chemical fungicides has necessitated the development of safe and eco-frie... more The impact of hazard by chemical fungicides has necessitated the development of safe and eco-friendly bio-fungicides. Trichoderma asperellum is one of the potent biocontrol agents and for its cost-effectiveness large scale production on several agricultural and industrial wastes has been tested so far. Forest fire due to highly inflammable pine needles and invasion of weeds are considered as a major cause of biodiversity loss in forests of NW Himalaya. Therefore, in the present work, the potential of forest wastes like needles of five conifer species and foliage of three noxious weeds along with some commonly used substrates were evaluated for the mass production of T. asperellum. Amongst all the screened substrates, the highest spore count was recorded on Pinus roxburghii needle powder supplemented with yeast extract and potato dextrose broth. Therefore, authors recommend the use of P. roxburghii needles for the mass multiplication of Trichoderma species as a replacement for useful agro-wastes.

BioNanoScience- Springer nature, 2023

The utilization of Trichoderma species-based formulations enables the eco-friendly management of ... more The utilization of Trichoderma species-based formulations enables the eco-friendly management of several fungal phytopathogens. These free living, filamentous fungi are cosmopolitan in their distribution, found in soils of natural and cultivated plant habitats, and they can be easily isolated and maintained under laboratory conditions. With the aid of TrichoBLAST and TrichoKEY, the identification of the isolates of this genus is easy to achieve and precise. They have enriched metabolomics resulting from their wide ecological versatility, strengthening their antimycotic potential by reinforcing mechanisms like hyperparasitism, antibiosis, and competition. Several commercial products synthesized from Trichoderma species demonstrate significant antifungal efficacy against numerous fungal phytopathogens. The global scientific communities are actively engaged in exploring innovative technologies to maximize their biocontrol potential and overcome the challenge of limited shelf life inherent in most Trichoderma-based commercial products. The emerging fusion of nanotechnology and Trichoderma species can be promising prospect in this regard, unlocking new avenues in the field of plant protection. As rich source of secondary metabolites, this genus holds immense potential for the synthesis of several nanoparticles with synergistic antifungal potential. They can serve as new generation of fungicides within nano range known as “nanofungicides.” They could be useful as an eco-friendly substitute against hazardous chemical fungicides after careful long-term monitoring of their mechanism of action, and risks associated with genotoxicity, phytotoxicity, etc. This study provides readers with a comprehensive exploration of Trichoderma species, ranging from their identification, to their utilization in field of nanotechnology for enhanced plant protection.

Mushroom Research, Aug 1, 2023

Fourteen wild edible and medicinal mushrooms representing ten families were collected from an alt... more Fourteen wild edible and medicinal mushrooms representing ten families were collected from an altitude range of 1,867-4,187 metres above mean sea level form Kinnaur district of Himachal Pradesh, NW Himalaya. The biochemical analysis of sporocarps revealed that the carbohydrate content was the maximum in Coprinus comatus and protein was highest in Auricularia auricula-judae, while the fibre content was recorded highest in Ganoderma lucidum. The energy value was determined using the amount of carbohydrates, protein, and fats present in the sporocarps and was found highest in Helvella lacunosa and lowest in G. lucidum. The ash content of sporocarps was used to estimate the mineral composition and was found highest in Sparassis crispa, closely followed by Lycoperdon perlatum, Russula brevipes, and lowest in R. stricta. The potassium, phosphorus, magnesium and calcium content in general were recorded higher in majority of mushrooms as compared to zinc, manganese, copper, etc. Despite the variations in nutrient content, these mushrooms appear to have a good overall nutritional profile. They have the potential to greatly fulfil people's requirement of proteins, macro and micronutrients. Therefore, mushrooms may be included in our diets on a regular basis to improve its quality.

International Journal of Farm Sciences, 2023

Nusantara bioscience, Mar 25, 2022

Diversity of arbuscular mycorrhizal fungi (AMF) associated with Polygonatum verticillatum (L.) Al... more Diversity of arbuscular mycorrhizal fungi (AMF) associated with Polygonatum verticillatum (L.) All. was investigated in two sites of Himachal Himalaya, India. A total of 15 AMF species were isolated and identified from the rhizosphere soil of P. verticillatum. The spore density was 1.48 ± 1.91 and 3.99 ± 3.78 per 20 gram of rhizosphere soil at site-I and site-II, respectively. Mycorrhizal colonization in the roots of P. verticillatum was recorded 46.12 and 52.23 percent at site-I and site-II, respectively. The mycorrhizal structures like darkly stained endophytic hyphae, coiled intracellular hyphae, Y-shaped hyphae and 'H' connection (Arum type) were recorded.

International journal of plant pathology, Jun 15, 2012

BioNanoScience, Aug 11, 2023

Biodiversitas, 1970

A study was conducted in Jeypore Reserve Forest located in Assam, India to investigate the divers... more A study was conducted in Jeypore Reserve Forest located in Assam, India to investigate the diversity of macrofungi associated with different tree species. The diversity of broad leaves trees and high humidity during monsoon period favours ideal growth of diverse group of macrofungal fruiting bodies. Thirty macrofungal species representing 26 genera belonging to 17 families were collected from six different sites in the study area. Out of these maximum six genera assignable to family Polyporaceae, five genera to Russulaceae, three genera to Agaricaceae, two genera to Ganodermataceae and Cantharellaceae each and rest of the families were represented by single genus only. The ecological preference of the species reveled that maximum (17) species were saprophyte, living on dead substrates or decaying wood debris, ten species were found associated with roots of higher trees, while three species were found parasitic. Overall 20 species were found edible including some species having medicinal utilization. The present study revealed that maximum frequency of occurrence was exhibited by Trametes versicolor and Schizophyllum commune (83.33%), followed by Microporus xanthopus, Pycnoporus sanguineus (66.67%) and Coprinus disseminates (50%). The rest of the species exhibited the frequency distribution ranging between 16.67-33.33%. The maximum density was recorded for Schizophyllum commune (126.67%) followed by Trametes versicolor (120%) and Xylaria polymorpha (93.33%). The density of rest of the species were ranged between 3.33-6.67%. The key objective of the present study was to generate a database on macrofungal diversity of Jeypore Reserve Forest along with their ecological preferences and utilization, which is not earlier documented.

Aging is a complicated biological process in which functional and structural alterations in a liv... more Aging is a complicated biological process in which functional and structural alterations in a living organism take place over time. Reactive oxygen species is one of the main factors responsible for aging and is associated with several chronic pathologies. The relationship between aging and diet is quite interesting and has attained worldwide attention. Healthy food, in addition to dietary antioxidants, are required to delay the process of aging and improve the quality of life. Many healthy foods such as fruits are a good source of dietary nutrients and natural bioactive compounds which have antioxidant properties and are involved in preventing aging and other age-related disorders. Health benefits linked with healthy consumption of fruit have drawn increased interest. A significant number of studies have documented the advantages of fruit intake, as it suppresses free-radical development that further reduces the oxidative stress created in the body and protects against several types of diseases such as cancer, type 2 diabetes, inflammatory disorders, and other cardiovascular diseases that ultimately prevent aging. In addition, fruits have numerous other properties like anti-inflammatory, anti-cancerous, anti-diabetic, neuroprotective, and have health-promoting effects. Mechanisms of various bioactive compounds that aids in preventing various diseases and increases longevity are also described. This manuscript provides a summary of various bioactive components present in fruits along with their health-promoting and antiaging properties.

Indian Journal of Forestry

An experiment was conducted to study the influence of seed sources on seed germination and seedli... more An experiment was conducted to study the influence of seed sources on seed germination and seedling vigour of Pinus gerardiana seeds collected from 23 seed sources during October, 2018 in Kinnaur and Chamba districts of Himachal Pradesh. The seeds were subjected to germination testing to identify the best seed source for raising Pinus gerardiana seedlings in the nursery. Significant differences were observed in germination percent, average seedling length and seedling vigour of seeds collected from different seed sources. The maximum germination of 83.00% was recorded in seeds collected from Jangi seed source followed by 74.00% germination in seeds collected from Purbani, 71.00% germination in seeds collected from Moorang in Kinnaur Forest Division whereas minimum germination of 36.00% germination was recorded in seeds collected from Luj in Pangi Forest Division. Similarly, maximum seedling length of 11.10 cm was recorded in seeds collected from Jangi followed by 10.50 cm in seeds c...

Nanofabrication

In the present research, a chemical co-precipitation approach has been used to approach the synth... more In the present research, a chemical co-precipitation approach has been used to approach the synthesis, characterization, and photocatalytic applicability of Ni-doped α-Fe2O3 (hematite) nanoparticles. Biosynthesized iron oxide nanoparticles (IONPs) were successfully synthesized using a non-toxic leaf extract of the Azadirachta indica (AI) plant (neem) as a reducing and stabilizing agent. X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), Raman spectroscopy, FT-IR spectroscopy, ultraviolet-visible (UV-Vis) spectroscopy, and vibrating sample magnetometer (VSM) have all been used to examine the synthesized materials. All of the produced NPs contain only the nanocrystalline hematite phase, according to XRD measurements. The morphology studies of the Ni-doping hematite nanoparticles, as demonstrated by TEM and SEM. The phase purity and phonon modes of the prepared nanoparticles are confirmed by Raman spectroscopy. The UV-Vis absorption tes...