shefali thakkar | Institute of Chemical Technology, Mumbai (original) (raw)
Strong research professional with a PhD (pursuing)in Pharmacognosy from Institute of Chemical Technology. Inclination towards various Extraction and Isolation techniques for the phytoconstituents, their analysis and characterization.
Address: Mumbai, Maharashtra, India
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Papers by shefali thakkar
Increased demand of Asphaltum (Shilajit) in immunity modulation and fertility has generated inten... more Increased demand of Asphaltum (Shilajit) in immunity modulation and fertility has generated intense interest among the producers and regulators to ensure its quality. For doing so, there is a need to isolate its chemical markers and analyze the same. Urolithins (Benzocoumarins) are one of the bioactive phytoconstituents in Asphaltum. Urolithin A and B are found to be present in Shilajit. The objective of this study was to develop a simple method for isolation of Urolithin A and B from Asphaltum. Elementary solvent partitioning technique was used for the extraction and isolation of Urolithins. Organic solvents like Methanol, Ethyl acetate, Petroleum ether etc were used. The isolated compounds were identified and characterized by TLC (Thin Layer Chromatography), UV (Ultra-violet), IR (Infrared) and MS (Mass) spectral analysis and purity was confirmed by HPLC (High Performance Liquid Chromatography) analysis. The isolated markers Urolithin A shows absorption maxima at 280, retention fa...
Journal of Pharmacognosy and Phytochemistry
Increased demand of Asphaltum (Shilajit) in immunity modulation and fertility has generated inten... more Increased demand of Asphaltum (Shilajit) in immunity modulation and fertility has generated intense interest among the producers and regulators to ensure its quality. For doing so, there is a need to isolate its chemical markers and analyze the same. Urolithins (Benzocoumarins) are one of the bioactive phytoconstituents in Asphaltum. Urolithin A and B are found to be present in Shilajit. The objective of this study was to develop a simple method for isolation of Urolithin A and B from Asphaltum. Elementary solvent partitioning technique was used for the extraction and isolation of Urolithins. Organic solvents like Methanol, Ethyl acetate, Petroleum ether etc were used. The isolated compounds were identified and characterized by TLC (Thin Layer Chromatography), UV (Ultra-violet), IR (Infrared) and MS (Mass) spectral analysis and purity was confirmed by HPLC (High Performance Liquid Chromatography) analysis. The isolated markers Urolithin A shows absorption maxima at 280, retention fa...
Journal of Pharmacognosy and Phytochemistry