Peter Maroy | Universitiy of Szeged (original) (raw)

Papers by Peter Maroy

Research paper thumbnail of Chromene derivatives and pesticidal composition comprising the same

Research paper thumbnail of Reduced expression of CDP-DAG synthase changes lipid composition and leads to male sterility in Drosophila

Open biology, 2016

Drosophila spermatogenesis is an ideal system to study the effects of changes in lipid compositio... more Drosophila spermatogenesis is an ideal system to study the effects of changes in lipid composition, because spermatid elongation and individualization requires extensive membrane biosynthesis and remodelling. The bulk of transcriptional activity is completed with the entry of cysts into meiotic division, which makes post-meiotic stages of spermatogenesis very sensitive to even a small reduction in gene products. In this study, we describe the effect of changes in lipid composition during spermatogenesis using a hypomorphic male sterile allele of the Drosophila CDP-DAG synthase (CdsA) gene. We find that the CdsA mutant shows defects in spermatid individualization and enlargement of mitochondria and the axonemal sheath of the spermatids. Furthermore, we could genetically rescue the male sterile phenotype by overexpressing Phosphatidylinositol synthase (dPIS) in a CdsA mutant background. The results of lipidomic and genetic analyses of the CdsA mutant highlight the importance of correc...

Research paper thumbnail of Chromene derivatives useful as insecticides

Research paper thumbnail of Process for the preparation of chromene derivatives

Research paper thumbnail of The occurence of two lohmanniids in Hungary

Research paper thumbnail of A new Hypoztes, Balogh species from the Great Hungarian Plain

Jl easurements.-length 544 fJ-' width;) 76 fl.. Prodorsum.-Rostrum (Fig. Ib) projected, but not s... more Jl easurements.-length 544 fJ-' width;) 76 fl.. Prodorsum.-Rostrum (Fig. Ib) projected, but not sharp, rounded at its apex with apical teeth. Rostral hair strong, superficially ciliate, incun-ed, arising at half distance between apex and bothrydium. Lamella situated parallel with margin of propodosoma in a marginal position, its inner margin attached to propodosoma, its tip truncate; lamellar hair originating below cuspis at an obtuse angle to lamella, directed towards and exceeding tip of rostral hair, ciliate. Dorsosejugal suture in midcUe protruding stI"Ongly onto propodosoma, bearing lamellar hairs. Strong lamellar hairs with fine cilia, their length twice that of prodorsum. Head of sensillus slightly fusiform. sparsely covered with fine hairs, its petiole one and a half times as long as head, proclinate to exclinate. Sensillus longer than lamellar hair. Totogaster. (Fig lc)-Ten pairs of finely ciliate hairs on notogaster. Hair ti proclinate, hair 1nS reclinate, all others radially exclinate. Setae P2 and P3 as long as r l , except for shorter Pl'~llarge obtusely trapewid, pale area on anterior portion of notogaster, behind dorsosejugal suture.-:lnogenital region.-Genital plate 'Yider anteriorly, with 6 pairs of hairs: anterior 3 pairs situated close to anterior margin of genital plate, posterior 3 pairs arranged in a longitudinal ro,Y. Anal aperture ,,-ider posteriorly, 2 pairs of anal hairs. Adanal slit iad situated parallel to lateral margin of anal aperture. Legs.-Tarsus tridactyle. Each tibia and genu with 1 thickned blunt seta on external side. Genu I (Fig. la) with a lamellar crest, showing a protruding tip. in a lateral view. Remark: Three species of Hypozetes BALOGHhave been hitherto described: H. imitator BALOGHfrom East Africa, H. bltlgaricus JELEVAfrom Bulgaria, and H. laysanensis .lORI from the Hawaiian Islands. H. inexpectatus sp. n. is the fourth species and its principal differences can be summarized as follmys: 1. The sensillus of H. imitator is shorter than lamellar hair and that of H. bulgaricus and H. laysanensis equal to it. The sensillus of H. inexpectatus distinctly longer than lamellar hair.-2. The rostral hair of H. bulgaricus and H. laysanensis is ciliate bilaterally while that of H. imitator and H. inexpectatus only on the external side.-3. The interlamellar hairs of H. imitator and H. laysanensis are short, not reaching the tip of the lamellar hairs, while those of H. bulgaricus and H. inexpectatus are markedly long and projecting far beyond the latter.-4. H. inexpectatI~s is longer than the formerly described species. The ratio of body length to body width (1.44) is rather similar to that of H. imitator (1.45).

Research paper thumbnail of Study of ecdysone-like action of transformed polyoxysteroids

Research paper thumbnail of Synhesis and use of pentafluorophenyl 6-(biotinylamido)hexanoate an alternative reagent for labelling of proteins with biotin moiety

The protein biotinylating agent, pentafluorophenyl 6-(biotinylamido)hexanoate (3) was syntheSized... more The protein biotinylating agent, pentafluorophenyl 6-(biotinylamido)hexanoate (3) was syntheSized. A straIghtforward synthetic route, biotin, biotin pentafluorophenyl ester (1), 6-(blOtlllylan-.udo)hexanoate (2) and pentafluorophenyl 6-(biotinylamido)hexanoate (3) was followed. This agent was used for biotin-labelling of different proteins, bovine serum albumin immunoglobulin, horseradish peroxidase and p-galactosidase. Optimal conditions for the la: belling were determined, as well as the influence of biotin-tagging to the biological activity of the proteins. An optimal ratio of the b.iotinylating agent to protein resulted in effective biotiny-latlOn WIthout a slglllficant decrease III the activity of the labelled enzyme. The labelled proteins proved to be suitable for immunodetection of proteins in both microplate and Western blot ImmunohistochemIstry, as well as in a competitive steroid ELISA of testosterone and 20-hydroxyecdysone.

Research paper thumbnail of ChemInform Abstract: Synthesis and Use of Pentafluorophenyl 6-(Biotinylamido)hexanoate. An Alternative Reagent for Labeling of Proteins with Biotin Moiety

ChemInform, 1993

ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance t... more ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

Research paper thumbnail of Synthesis and Use of Pentafluorophenyl 6-(Biotinylamido)hexanoate An Alternative Reagent for Labelling of Proteins with Biotin Moiety

Zeitschrift für Naturforschung B, 1993

The protein biotinylating agent, pentafluorophenyl 6-(biotinylam ido)hexanoate (3) was synthesize... more The protein biotinylating agent, pentafluorophenyl 6-(biotinylam ido)hexanoate (3) was synthesized. A straightforward synthetic route, biotin, biotin pentafluorophenyl ester (1), 6-(biotinylam ido)hexanoate (2) and pentafluorophenyl 6-(biotinylam ido)hexanoate (3) was followed. This agent was used for biotin-labelling of different proteins, bovine serum album in, immunoglobulin, horseradish peroxidase and β-galactosidase. Optimal conditions for the labelling were determined, as well as the influence of biotin-tagging to the biological activity of the proteins. An optimal ratio of the biotinylating agent to protein resulted in effective biotinylation without a significant decrease in the activity of the labelled enzyme. The labelled proteins proved to be suitable for immunodetection of proteins in both microplate and Western blot immunohistochemistry, as well as in a competitive steroid ELISA of testosterone and 20-hydroxyecdysone.

Research paper thumbnail of Hormones and Sex-Specific Transcription Factors Jointly Control Yolk Protein Synthesis in Musca domestica

International Journal of Evolutionary Biology, 2009

In the houseflyMusca domestica, synthesis of yolk proteins (YPs) depends on the level of circulat... more In the houseflyMusca domestica, synthesis of yolk proteins (YPs) depends on the level of circulating ecdysteroid hormones. In female houseflies, the ecdysterone concentration in the hemolymph oscillates and, at high levels, is followed by expression of YP. In male houseflies, the ecdysterone titre is constantly low and no YP is produced. In some strains, which are mutant in key components of the sex-determining pathway, males express YP even though their ecdysterone titre is not significantly elevated. However, we find that these males express a substantial amount of the female variant of theMusca doublesexhomologue,Md-dsx. Thedsxgene is known to sex-specifically control transcription ofypgenes in the fat body ofDrosophila melanogaster. Our data suggest thatMd-dsxalso contributes to the regulation of YP expression in the housefly by modulating the responsiveness of YP-producing cells to hormonal stimuli.

Research paper thumbnail of SNF4Aγ, the Drosophila AMPK γ subunit is required for regulation of developmental and stress-induced autophagy

Autophagy, 2008

This manuscript has been published online, prior to printing.Once the issue is complete and page ... more This manuscript has been published online, prior to printing.Once the issue is complete and page numbers have been assigned, the citation will change accordingly.

Research paper thumbnail of Ecdysteroid Receptors in Cultured Drosophila Cells

Gene Regulation by Steroid Hormones, 1980

Research paper thumbnail of The uptake and release of ponasterone a by the Kc cell line of Drosophila melanogaster

Molecular and Cellular Endocrinology, 1980

The association of ponasterone A (PNA) and 20-hydroxyecdysone with Kc cells is commensurate with ... more The association of ponasterone A (PNA) and 20-hydroxyecdysone with Kc cells is commensurate with their biological activity on this Kc cell line, the physiological activity ratio for PNA, 20-hydroxyecdysone and ecdysone is 1 : 50 : 2000, resp. Both association and release of [3H]-PNA are temperature-dependent, the activation energy was calculated as 16.7 caJ (Arrhenius analysis). This association is compatible with unlabelled PNA and various ecdysteroids. The KD for PNA (Scatchard analysis) was estimated as 3.6 X 10e9 M, giving the number of binding sites as approx. 1800 per cell.

Research paper thumbnail of Ecdysteroid receptors in the central nervous system ofManduca sexta: Their changes in distribution and quantity during larval-pupal development

The Journal of Comparative Neurology, 1991

Research paper thumbnail of Rapid heterologous haptene radioimmunoassay for insect moulting hormone

FEBS Letters, 1977

Several recent studies suggest that moulting hormone (MH) is involved in regulating both moulting... more Several recent studies suggest that moulting hormone (MH) is involved in regulating both moulting and ecdysis in insects. Although this hormone has been shown to effect dramatic changes in the metabolism of macromolecules, little is known about the mechanisms by ...

Research paper thumbnail of SNF4A gamma, the Drosophila AMPK gamma subunit is required for regulation of developmental and stress-induced autophagy

Autophagy, 2008

This manuscript has been published online, prior to printing.Once the issue is complete and page ... more This manuscript has been published online, prior to printing.Once the issue is complete and page numbers have been assigned, the citation will change accordingly.

Research paper thumbnail of P-Element Insertion Alleles of Essential Genes on the Third Chromosome of Drosophila Melanogaster: Correlation of Physical and Cytogenetic Maps In Chromosomal …

Genetics, 1997

We have established a collection of 2460 lethal or semi-lethal mutant lines using a procedure tho... more We have established a collection of 2460 lethal or semi-lethal mutant lines using a procedure thought to insert single P elements into vital genes on the third chromosome of Drosophila melanogaster. More than 1200 randomly selected lines were examined by in situ hybridization and 90% found to contain single insertions at sites that mark 89% of all lettered subdivisions of the Bridges' map. A set of chromosomal deficiencies that collectively uncover-25% of the euchromatin of chromosome 3 reveal lethal mutations in 468 lines corresponding to 145 complementation groups. We undertook a detailed analysis of the cytogenetic interval 86E-87F and identified 87 P-element-induced mutations falling into 38 complementation groups, 16 of which correspond to previously known genes. Twenty-one of these 38 complementation groups have at least one allele that has a Pelement insertion at a position consistent with the cytogenetics of the locus. We have rescued P elements and flanking chromosomal sequences from the 86E-87F region in 35 lines with either lethal or genetically silent P insertions, and used these as probes to identify cosmids and P1 clones from the Drosophila genome projects. This has tied together the physical and genetic maps and has linked 44 previously identified cosmid contigs into seven "supercontigs" that span the interval. STS data for sequences flanking one side of the P-element insertions in 49 lines has identified insertions in the ay element at 87C, two known transposable elements, and the open reading frames of seven putative single copy genes. These correspond to five known genes in this interval, and two genes identified by the homology of their predicted products to known proteins from other organisms.

Research paper thumbnail of About the oribatid fauna of the Tisza basin

The paner contains an enumeration of some oribatid mites discovered during investigation of the s... more The paner contains an enumeration of some oribatid mites discovered during investigation of the soil hardwood and softwood groves. The investigations took place in ten different collecting stations where S3 mite species w~r~discovered. Three species of them. Epidama~u~a66i~i~, M~ta-b~lba mon~l~p~da, Eup~lop~u~ac~u. were so far not demonstrated in the fauna of the Carpathian basin. The naoer contains also a short descrintioõ f these species.

Research paper thumbnail of Ecdysteroid receptors in Drosophila melanogaster adult females

Molecular and Cellular Endocrinology, 1989

Ecdysteroid receptors were identified and partially characterized from total cell extracts of who... more Ecdysteroid receptors were identified and partially characterized from total cell extracts of whole animals and dissected tissues from Drosophila melunogaster adult females. Binding studies indicated the presence of two ecdysteroid binding components having high affinity and specificity consistent with receptors previously identified in embryos and larvae. The highest affinity binding component in 3-to 4-day females had a dissociation constant of 9.2 x lo-lo M and a maximal binding concentration of approximately 90 pmol/g protein, with a lower affinity component having a dissociation constant of 2.94 X 10e9 M. Receptors at similar concentrations were also observed in abdominal walls containing adult fat body, with relatively lower receptor levels observed in ovaries. These results indicate that the observed ecdysteroid hormone concentrations in adult females can account for a physiological stimulator-y effect on yolk protein synthesis in adult fat body.

Research paper thumbnail of Chromene derivatives and pesticidal composition comprising the same

Research paper thumbnail of Reduced expression of CDP-DAG synthase changes lipid composition and leads to male sterility in Drosophila

Open biology, 2016

Drosophila spermatogenesis is an ideal system to study the effects of changes in lipid compositio... more Drosophila spermatogenesis is an ideal system to study the effects of changes in lipid composition, because spermatid elongation and individualization requires extensive membrane biosynthesis and remodelling. The bulk of transcriptional activity is completed with the entry of cysts into meiotic division, which makes post-meiotic stages of spermatogenesis very sensitive to even a small reduction in gene products. In this study, we describe the effect of changes in lipid composition during spermatogenesis using a hypomorphic male sterile allele of the Drosophila CDP-DAG synthase (CdsA) gene. We find that the CdsA mutant shows defects in spermatid individualization and enlargement of mitochondria and the axonemal sheath of the spermatids. Furthermore, we could genetically rescue the male sterile phenotype by overexpressing Phosphatidylinositol synthase (dPIS) in a CdsA mutant background. The results of lipidomic and genetic analyses of the CdsA mutant highlight the importance of correc...

Research paper thumbnail of Chromene derivatives useful as insecticides

Research paper thumbnail of Process for the preparation of chromene derivatives

Research paper thumbnail of The occurence of two lohmanniids in Hungary

Research paper thumbnail of A new Hypoztes, Balogh species from the Great Hungarian Plain

Jl easurements.-length 544 fJ-' width;) 76 fl.. Prodorsum.-Rostrum (Fig. Ib) projected, but not s... more Jl easurements.-length 544 fJ-' width;) 76 fl.. Prodorsum.-Rostrum (Fig. Ib) projected, but not sharp, rounded at its apex with apical teeth. Rostral hair strong, superficially ciliate, incun-ed, arising at half distance between apex and bothrydium. Lamella situated parallel with margin of propodosoma in a marginal position, its inner margin attached to propodosoma, its tip truncate; lamellar hair originating below cuspis at an obtuse angle to lamella, directed towards and exceeding tip of rostral hair, ciliate. Dorsosejugal suture in midcUe protruding stI"Ongly onto propodosoma, bearing lamellar hairs. Strong lamellar hairs with fine cilia, their length twice that of prodorsum. Head of sensillus slightly fusiform. sparsely covered with fine hairs, its petiole one and a half times as long as head, proclinate to exclinate. Sensillus longer than lamellar hair. Totogaster. (Fig lc)-Ten pairs of finely ciliate hairs on notogaster. Hair ti proclinate, hair 1nS reclinate, all others radially exclinate. Setae P2 and P3 as long as r l , except for shorter Pl'~llarge obtusely trapewid, pale area on anterior portion of notogaster, behind dorsosejugal suture.-:lnogenital region.-Genital plate 'Yider anteriorly, with 6 pairs of hairs: anterior 3 pairs situated close to anterior margin of genital plate, posterior 3 pairs arranged in a longitudinal ro,Y. Anal aperture ,,-ider posteriorly, 2 pairs of anal hairs. Adanal slit iad situated parallel to lateral margin of anal aperture. Legs.-Tarsus tridactyle. Each tibia and genu with 1 thickned blunt seta on external side. Genu I (Fig. la) with a lamellar crest, showing a protruding tip. in a lateral view. Remark: Three species of Hypozetes BALOGHhave been hitherto described: H. imitator BALOGHfrom East Africa, H. bltlgaricus JELEVAfrom Bulgaria, and H. laysanensis .lORI from the Hawaiian Islands. H. inexpectatus sp. n. is the fourth species and its principal differences can be summarized as follmys: 1. The sensillus of H. imitator is shorter than lamellar hair and that of H. bulgaricus and H. laysanensis equal to it. The sensillus of H. inexpectatus distinctly longer than lamellar hair.-2. The rostral hair of H. bulgaricus and H. laysanensis is ciliate bilaterally while that of H. imitator and H. inexpectatus only on the external side.-3. The interlamellar hairs of H. imitator and H. laysanensis are short, not reaching the tip of the lamellar hairs, while those of H. bulgaricus and H. inexpectatus are markedly long and projecting far beyond the latter.-4. H. inexpectatI~s is longer than the formerly described species. The ratio of body length to body width (1.44) is rather similar to that of H. imitator (1.45).

Research paper thumbnail of Study of ecdysone-like action of transformed polyoxysteroids

Research paper thumbnail of Synhesis and use of pentafluorophenyl 6-(biotinylamido)hexanoate an alternative reagent for labelling of proteins with biotin moiety

The protein biotinylating agent, pentafluorophenyl 6-(biotinylamido)hexanoate (3) was syntheSized... more The protein biotinylating agent, pentafluorophenyl 6-(biotinylamido)hexanoate (3) was syntheSized. A straIghtforward synthetic route, biotin, biotin pentafluorophenyl ester (1), 6-(blOtlllylan-.udo)hexanoate (2) and pentafluorophenyl 6-(biotinylamido)hexanoate (3) was followed. This agent was used for biotin-labelling of different proteins, bovine serum albumin immunoglobulin, horseradish peroxidase and p-galactosidase. Optimal conditions for the la: belling were determined, as well as the influence of biotin-tagging to the biological activity of the proteins. An optimal ratio of the b.iotinylating agent to protein resulted in effective biotiny-latlOn WIthout a slglllficant decrease III the activity of the labelled enzyme. The labelled proteins proved to be suitable for immunodetection of proteins in both microplate and Western blot ImmunohistochemIstry, as well as in a competitive steroid ELISA of testosterone and 20-hydroxyecdysone.

Research paper thumbnail of ChemInform Abstract: Synthesis and Use of Pentafluorophenyl 6-(Biotinylamido)hexanoate. An Alternative Reagent for Labeling of Proteins with Biotin Moiety

ChemInform, 1993

ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance t... more ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

Research paper thumbnail of Synthesis and Use of Pentafluorophenyl 6-(Biotinylamido)hexanoate An Alternative Reagent for Labelling of Proteins with Biotin Moiety

Zeitschrift für Naturforschung B, 1993

The protein biotinylating agent, pentafluorophenyl 6-(biotinylam ido)hexanoate (3) was synthesize... more The protein biotinylating agent, pentafluorophenyl 6-(biotinylam ido)hexanoate (3) was synthesized. A straightforward synthetic route, biotin, biotin pentafluorophenyl ester (1), 6-(biotinylam ido)hexanoate (2) and pentafluorophenyl 6-(biotinylam ido)hexanoate (3) was followed. This agent was used for biotin-labelling of different proteins, bovine serum album in, immunoglobulin, horseradish peroxidase and β-galactosidase. Optimal conditions for the labelling were determined, as well as the influence of biotin-tagging to the biological activity of the proteins. An optimal ratio of the biotinylating agent to protein resulted in effective biotinylation without a significant decrease in the activity of the labelled enzyme. The labelled proteins proved to be suitable for immunodetection of proteins in both microplate and Western blot immunohistochemistry, as well as in a competitive steroid ELISA of testosterone and 20-hydroxyecdysone.

Research paper thumbnail of Hormones and Sex-Specific Transcription Factors Jointly Control Yolk Protein Synthesis in Musca domestica

International Journal of Evolutionary Biology, 2009

In the houseflyMusca domestica, synthesis of yolk proteins (YPs) depends on the level of circulat... more In the houseflyMusca domestica, synthesis of yolk proteins (YPs) depends on the level of circulating ecdysteroid hormones. In female houseflies, the ecdysterone concentration in the hemolymph oscillates and, at high levels, is followed by expression of YP. In male houseflies, the ecdysterone titre is constantly low and no YP is produced. In some strains, which are mutant in key components of the sex-determining pathway, males express YP even though their ecdysterone titre is not significantly elevated. However, we find that these males express a substantial amount of the female variant of theMusca doublesexhomologue,Md-dsx. Thedsxgene is known to sex-specifically control transcription ofypgenes in the fat body ofDrosophila melanogaster. Our data suggest thatMd-dsxalso contributes to the regulation of YP expression in the housefly by modulating the responsiveness of YP-producing cells to hormonal stimuli.

Research paper thumbnail of SNF4Aγ, the Drosophila AMPK γ subunit is required for regulation of developmental and stress-induced autophagy

Autophagy, 2008

This manuscript has been published online, prior to printing.Once the issue is complete and page ... more This manuscript has been published online, prior to printing.Once the issue is complete and page numbers have been assigned, the citation will change accordingly.

Research paper thumbnail of Ecdysteroid Receptors in Cultured Drosophila Cells

Gene Regulation by Steroid Hormones, 1980

Research paper thumbnail of The uptake and release of ponasterone a by the Kc cell line of Drosophila melanogaster

Molecular and Cellular Endocrinology, 1980

The association of ponasterone A (PNA) and 20-hydroxyecdysone with Kc cells is commensurate with ... more The association of ponasterone A (PNA) and 20-hydroxyecdysone with Kc cells is commensurate with their biological activity on this Kc cell line, the physiological activity ratio for PNA, 20-hydroxyecdysone and ecdysone is 1 : 50 : 2000, resp. Both association and release of [3H]-PNA are temperature-dependent, the activation energy was calculated as 16.7 caJ (Arrhenius analysis). This association is compatible with unlabelled PNA and various ecdysteroids. The KD for PNA (Scatchard analysis) was estimated as 3.6 X 10e9 M, giving the number of binding sites as approx. 1800 per cell.

Research paper thumbnail of Ecdysteroid receptors in the central nervous system ofManduca sexta: Their changes in distribution and quantity during larval-pupal development

The Journal of Comparative Neurology, 1991

Research paper thumbnail of Rapid heterologous haptene radioimmunoassay for insect moulting hormone

FEBS Letters, 1977

Several recent studies suggest that moulting hormone (MH) is involved in regulating both moulting... more Several recent studies suggest that moulting hormone (MH) is involved in regulating both moulting and ecdysis in insects. Although this hormone has been shown to effect dramatic changes in the metabolism of macromolecules, little is known about the mechanisms by ...

Research paper thumbnail of SNF4A gamma, the Drosophila AMPK gamma subunit is required for regulation of developmental and stress-induced autophagy

Autophagy, 2008

This manuscript has been published online, prior to printing.Once the issue is complete and page ... more This manuscript has been published online, prior to printing.Once the issue is complete and page numbers have been assigned, the citation will change accordingly.

Research paper thumbnail of P-Element Insertion Alleles of Essential Genes on the Third Chromosome of Drosophila Melanogaster: Correlation of Physical and Cytogenetic Maps In Chromosomal …

Genetics, 1997

We have established a collection of 2460 lethal or semi-lethal mutant lines using a procedure tho... more We have established a collection of 2460 lethal or semi-lethal mutant lines using a procedure thought to insert single P elements into vital genes on the third chromosome of Drosophila melanogaster. More than 1200 randomly selected lines were examined by in situ hybridization and 90% found to contain single insertions at sites that mark 89% of all lettered subdivisions of the Bridges' map. A set of chromosomal deficiencies that collectively uncover-25% of the euchromatin of chromosome 3 reveal lethal mutations in 468 lines corresponding to 145 complementation groups. We undertook a detailed analysis of the cytogenetic interval 86E-87F and identified 87 P-element-induced mutations falling into 38 complementation groups, 16 of which correspond to previously known genes. Twenty-one of these 38 complementation groups have at least one allele that has a Pelement insertion at a position consistent with the cytogenetics of the locus. We have rescued P elements and flanking chromosomal sequences from the 86E-87F region in 35 lines with either lethal or genetically silent P insertions, and used these as probes to identify cosmids and P1 clones from the Drosophila genome projects. This has tied together the physical and genetic maps and has linked 44 previously identified cosmid contigs into seven "supercontigs" that span the interval. STS data for sequences flanking one side of the P-element insertions in 49 lines has identified insertions in the ay element at 87C, two known transposable elements, and the open reading frames of seven putative single copy genes. These correspond to five known genes in this interval, and two genes identified by the homology of their predicted products to known proteins from other organisms.

Research paper thumbnail of About the oribatid fauna of the Tisza basin

The paner contains an enumeration of some oribatid mites discovered during investigation of the s... more The paner contains an enumeration of some oribatid mites discovered during investigation of the soil hardwood and softwood groves. The investigations took place in ten different collecting stations where S3 mite species w~r~discovered. Three species of them. Epidama~u~a66i~i~, M~ta-b~lba mon~l~p~da, Eup~lop~u~ac~u. were so far not demonstrated in the fauna of the Carpathian basin. The naoer contains also a short descrintioõ f these species.

Research paper thumbnail of Ecdysteroid receptors in Drosophila melanogaster adult females

Molecular and Cellular Endocrinology, 1989

Ecdysteroid receptors were identified and partially characterized from total cell extracts of who... more Ecdysteroid receptors were identified and partially characterized from total cell extracts of whole animals and dissected tissues from Drosophila melunogaster adult females. Binding studies indicated the presence of two ecdysteroid binding components having high affinity and specificity consistent with receptors previously identified in embryos and larvae. The highest affinity binding component in 3-to 4-day females had a dissociation constant of 9.2 x lo-lo M and a maximal binding concentration of approximately 90 pmol/g protein, with a lower affinity component having a dissociation constant of 2.94 X 10e9 M. Receptors at similar concentrations were also observed in abdominal walls containing adult fat body, with relatively lower receptor levels observed in ovaries. These results indicate that the observed ecdysteroid hormone concentrations in adult females can account for a physiological stimulator-y effect on yolk protein synthesis in adult fat body.