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Papers by Gustavo Caetano-Anolles
Nature Biotechnology, 1994
The enzymatic amplification of DNA directed by very short oligonucleotides of arbitrary sequence ... more The enzymatic amplification of DNA directed by very short oligonucleotides of arbitrary sequence produces complex DNA profiles useful for genome analysis and identity testing. Mini-hairpins harboring a core arbitrary sequence at the 3′ terminus primed the amplification of a ...
Abstract: A method for visualizing nucleic acids in a polyacrylamide gel. The method comprises fi... more Abstract: A method for visualizing nucleic acids in a polyacrylamide gel. The method comprises fixing the nucleic acids with 10% acetic acid for about 20 minutes, washing the gel multiple times with water for about 2 minutes, impregnating the gel with silver nitrate at ...
Briefings in Bioinformatics, Jan 31, 2018
Methods in molecular and cellular biology, 1994
Springer eBooks, 2017
The origin and evolution of molecular functions hold the key to the emergence of modern biochemis... more The origin and evolution of molecular functions hold the key to the emergence of modern biochemistry and cellular organization. Here we explore the existence of a growing vocabulary in the proteins and molecular functions of Archaea. A genomic census of structural domains and its mappings to Gene Ontology terms provides the raw data for the search of meaningful patterns and processes that drive molecular change. We present evidence supporting the existence of statistical laws of language and socioeconomic-linked diffusion of innovation models intricately embedded in both protein structure and domain organization. Patterns of origin and diversification of organismal repertoires of proteins (proteomes) and functions (functionomes) reveal their makeup depends on trade-off solutions between three principles that favor organismal and molecular persistence: economy, flexibility and robustness. We find that the microbes of Archaea and Bacteria maximize economy, while eukaryotic organisms maximize flexibility and robustness. In the process, archaeal organisms engage in extreme semantic and pragmatic compression of their messages in response to evolutionary constraints, which were probably historically imposed by microbial lifestyle and harsh environments. Archaea preserves an economy-driven primordial vocabulary that is highly homogeneous and is the most ancient of the cellular world.
Plant and Soil, 1993
... GUSTAVO CAETANO-ANOLLI~S 1 and PETER M. GRESSHOFF Plant Molecular Genetics, Institute of Agri... more ... GUSTAVO CAETANO-ANOLLI~S 1 and PETER M. GRESSHOFF Plant Molecular Genetics, Institute of Agriculture and Center for Legume Research ... Pierce and Bauer (1983) showed the existence of an autoregulatory response that inhibits nodu-iation of distal portions of the ...
Evolutionary Bioinformatics, 2018
Applied Microbiology and Biotechnology, Oct 1, 1992
Nature Biotechnology, Jun 1, 1991
Analytical Biochemistry, Jul 1, 1991
The photochemically derived silver stain of nucleic acids in polyacrylamide gels originally descr... more The photochemically derived silver stain of nucleic acids in polyacrylamide gels originally described by Merril et al. (1981, Science 211, 1437-1438) was modified to reduce unspecific background staining and increase sensitivity (down to 1 pg/mm2 band cross-section). Detection limits for double-stranded DNA fragments from HaeIII endonuclease digests of phage phi X174 were maintained despite eliminating oxidation pretreatment of fixed gels and reducing silver nitrate concentration. Preexposure to formaldehyde during silver impregnation enhanced sensitivity and the inclusion of the silver-complexing agent sodium thiosulphate in the image developer decreased background staining. Higher formaldehyde concentration during image development resulted in darker bands with good contrast. The procedure almost halves the number of steps, solutions and experimental time required and can be used for the staining of DNA fragments in polyacrylamide gels bound to a polyester backing film by controlling temperature during image development. We have applied this improved staining procedure for the routine analysis of complex DNA profiles generated by DNA amplification fingerprinting (DAF).
Science Progress, Oct 1, 2018
Journal of Plant Physiology, Oct 1, 1991
Summary Nodulation in alfalfa ( Medicago sativa L.) is controlled by a systemic feedback regulato... more Summary Nodulation in alfalfa ( Medicago sativa L.) is controlled by a systemic feedback regulatory mechanism that suppresses nodule initiation in younger portions of the root system. Excision of primary root nodules induced by wild-type Rhizobium meliloti stimulates the formation of new nodules on lateral roots. In similar experiments we found that excision of bacteria-free nodules from primary roots induced by mutants of R. meliloti deficient in exopolysaccharide synthesis allowed nodules to reappear in lateral roots especially around the root tip at the time of nodule removal. Our results suggest that organized nodular structures trigger autoregulatory responses in legumes, even in the absence of bacterial infection.
BioTechniques, Jun 1, 1996
Plant Science, 1990
Separately grown soybean (Glycine max (L.) Merr. cv. Bragg) plants of identical or different nodu... more Separately grown soybean (Glycine max (L.) Merr. cv. Bragg) plants of identical or different nodulation genotype were approach-grafted just below the cotyledons. Five days later, their roots were inoculated at different times to study the effects of prior inoculation of one root system on nodulation ...
Springer eBooks, 1993
It is generally assumed that Rhizobium and Bradyrhizobium bacteria are the causative agent for no... more It is generally assumed that Rhizobium and Bradyrhizobium bacteria are the causative agent for nodulation in legumes. The analysis of bacterial genes and their products is focused on the elucidation of the signal-transduction pathway leading to cell division, differentiation and infection (Caetano-Anolles, Gresshoff, 1991a). Bacteria are chemoattracted to the root surface (Bauer, Caetano-Anolles, 1990) where plant flavonoids induce bacterial gene expression (Peters, Verma, 1990) and secretion of factors that trigger the first morphological responses in the host, root hair curling and cortical cell division (Denarie et al. 1992). Concurrent anticlinal and periclinal cell divisions, usually beneath the infection site, initiate a nodule primordium that forms a persistent nodule meristem in its distal end and gives rise to a central tissue with infected and uninfected cells.
Journal of Plant Physiology, Feb 1, 1991
Plant Physiology, Dec 1, 1993
Planta, Dec 1, 1991
A small subpopulation of alfalfa (Medicago saliva L.) plants grown without fixed nitrogen can dev... more A small subpopulation of alfalfa (Medicago saliva L.) plants grown without fixed nitrogen can develop root nodules in the absence of Rhizobium. Cytological studies showed that these nodules were organized structures with no inter- or intracellular bacteria but with the histological characteristics of a normal indeterminate nodule. Few if any viable bacteria were recovered from the nodules after surface sterilization, and when the nodular content was used to inoculate alfalfa roots no nodulation was observed. These spontaneous nodules were formed mainly on the primary roots in the region susceptible to Rhizobium infection between 4 and 6 d after seed imbibition. Spontaneous nodules appeared as early as 10 d after germination and emerged at a rate comparable to normal nodules. The formation of spontaneous nodules on the primary root suppressed nodulation in lateral roots after inoculation with R. meliloti RCR2011. Excision of spontaneous nodules at inoculation eliminated the suppressive response. Our results indicate that the presence of Rhizobium is not required for nodule organogenesis and the elicitation of feedback regulation of nodule formation in alfalfa.
Nature Biotechnology, Jun 1, 1994
Nature Biotechnology, 1994
The enzymatic amplification of DNA directed by very short oligonucleotides of arbitrary sequence ... more The enzymatic amplification of DNA directed by very short oligonucleotides of arbitrary sequence produces complex DNA profiles useful for genome analysis and identity testing. Mini-hairpins harboring a core arbitrary sequence at the 3′ terminus primed the amplification of a ...
Abstract: A method for visualizing nucleic acids in a polyacrylamide gel. The method comprises fi... more Abstract: A method for visualizing nucleic acids in a polyacrylamide gel. The method comprises fixing the nucleic acids with 10% acetic acid for about 20 minutes, washing the gel multiple times with water for about 2 minutes, impregnating the gel with silver nitrate at ...
Briefings in Bioinformatics, Jan 31, 2018
Methods in molecular and cellular biology, 1994
Springer eBooks, 2017
The origin and evolution of molecular functions hold the key to the emergence of modern biochemis... more The origin and evolution of molecular functions hold the key to the emergence of modern biochemistry and cellular organization. Here we explore the existence of a growing vocabulary in the proteins and molecular functions of Archaea. A genomic census of structural domains and its mappings to Gene Ontology terms provides the raw data for the search of meaningful patterns and processes that drive molecular change. We present evidence supporting the existence of statistical laws of language and socioeconomic-linked diffusion of innovation models intricately embedded in both protein structure and domain organization. Patterns of origin and diversification of organismal repertoires of proteins (proteomes) and functions (functionomes) reveal their makeup depends on trade-off solutions between three principles that favor organismal and molecular persistence: economy, flexibility and robustness. We find that the microbes of Archaea and Bacteria maximize economy, while eukaryotic organisms maximize flexibility and robustness. In the process, archaeal organisms engage in extreme semantic and pragmatic compression of their messages in response to evolutionary constraints, which were probably historically imposed by microbial lifestyle and harsh environments. Archaea preserves an economy-driven primordial vocabulary that is highly homogeneous and is the most ancient of the cellular world.
Plant and Soil, 1993
... GUSTAVO CAETANO-ANOLLI~S 1 and PETER M. GRESSHOFF Plant Molecular Genetics, Institute of Agri... more ... GUSTAVO CAETANO-ANOLLI~S 1 and PETER M. GRESSHOFF Plant Molecular Genetics, Institute of Agriculture and Center for Legume Research ... Pierce and Bauer (1983) showed the existence of an autoregulatory response that inhibits nodu-iation of distal portions of the ...
Evolutionary Bioinformatics, 2018
Applied Microbiology and Biotechnology, Oct 1, 1992
Nature Biotechnology, Jun 1, 1991
Analytical Biochemistry, Jul 1, 1991
The photochemically derived silver stain of nucleic acids in polyacrylamide gels originally descr... more The photochemically derived silver stain of nucleic acids in polyacrylamide gels originally described by Merril et al. (1981, Science 211, 1437-1438) was modified to reduce unspecific background staining and increase sensitivity (down to 1 pg/mm2 band cross-section). Detection limits for double-stranded DNA fragments from HaeIII endonuclease digests of phage phi X174 were maintained despite eliminating oxidation pretreatment of fixed gels and reducing silver nitrate concentration. Preexposure to formaldehyde during silver impregnation enhanced sensitivity and the inclusion of the silver-complexing agent sodium thiosulphate in the image developer decreased background staining. Higher formaldehyde concentration during image development resulted in darker bands with good contrast. The procedure almost halves the number of steps, solutions and experimental time required and can be used for the staining of DNA fragments in polyacrylamide gels bound to a polyester backing film by controlling temperature during image development. We have applied this improved staining procedure for the routine analysis of complex DNA profiles generated by DNA amplification fingerprinting (DAF).
Science Progress, Oct 1, 2018
Journal of Plant Physiology, Oct 1, 1991
Summary Nodulation in alfalfa ( Medicago sativa L.) is controlled by a systemic feedback regulato... more Summary Nodulation in alfalfa ( Medicago sativa L.) is controlled by a systemic feedback regulatory mechanism that suppresses nodule initiation in younger portions of the root system. Excision of primary root nodules induced by wild-type Rhizobium meliloti stimulates the formation of new nodules on lateral roots. In similar experiments we found that excision of bacteria-free nodules from primary roots induced by mutants of R. meliloti deficient in exopolysaccharide synthesis allowed nodules to reappear in lateral roots especially around the root tip at the time of nodule removal. Our results suggest that organized nodular structures trigger autoregulatory responses in legumes, even in the absence of bacterial infection.
BioTechniques, Jun 1, 1996
Plant Science, 1990
Separately grown soybean (Glycine max (L.) Merr. cv. Bragg) plants of identical or different nodu... more Separately grown soybean (Glycine max (L.) Merr. cv. Bragg) plants of identical or different nodulation genotype were approach-grafted just below the cotyledons. Five days later, their roots were inoculated at different times to study the effects of prior inoculation of one root system on nodulation ...
Springer eBooks, 1993
It is generally assumed that Rhizobium and Bradyrhizobium bacteria are the causative agent for no... more It is generally assumed that Rhizobium and Bradyrhizobium bacteria are the causative agent for nodulation in legumes. The analysis of bacterial genes and their products is focused on the elucidation of the signal-transduction pathway leading to cell division, differentiation and infection (Caetano-Anolles, Gresshoff, 1991a). Bacteria are chemoattracted to the root surface (Bauer, Caetano-Anolles, 1990) where plant flavonoids induce bacterial gene expression (Peters, Verma, 1990) and secretion of factors that trigger the first morphological responses in the host, root hair curling and cortical cell division (Denarie et al. 1992). Concurrent anticlinal and periclinal cell divisions, usually beneath the infection site, initiate a nodule primordium that forms a persistent nodule meristem in its distal end and gives rise to a central tissue with infected and uninfected cells.
Journal of Plant Physiology, Feb 1, 1991
Plant Physiology, Dec 1, 1993
Planta, Dec 1, 1991
A small subpopulation of alfalfa (Medicago saliva L.) plants grown without fixed nitrogen can dev... more A small subpopulation of alfalfa (Medicago saliva L.) plants grown without fixed nitrogen can develop root nodules in the absence of Rhizobium. Cytological studies showed that these nodules were organized structures with no inter- or intracellular bacteria but with the histological characteristics of a normal indeterminate nodule. Few if any viable bacteria were recovered from the nodules after surface sterilization, and when the nodular content was used to inoculate alfalfa roots no nodulation was observed. These spontaneous nodules were formed mainly on the primary roots in the region susceptible to Rhizobium infection between 4 and 6 d after seed imbibition. Spontaneous nodules appeared as early as 10 d after germination and emerged at a rate comparable to normal nodules. The formation of spontaneous nodules on the primary root suppressed nodulation in lateral roots after inoculation with R. meliloti RCR2011. Excision of spontaneous nodules at inoculation eliminated the suppressive response. Our results indicate that the presence of Rhizobium is not required for nodule organogenesis and the elicitation of feedback regulation of nodule formation in alfalfa.
Nature Biotechnology, Jun 1, 1994