mamta Chauhan | Institute of Management Studies, Dehradun (original) (raw)

Papers by mamta Chauhan

Research paper thumbnail of Use of Certain Naturally Ocurring Herbal Grain Protectants against Sitophilus oryzae Linn. (Coleoptera :Curculionidae

... (Coleoptera :Curculionidae) Sushil Kumar, Mamta Bhadauria, AKS Chauhan and BS Chandel* Deptt.... more ... (Coleoptera :Curculionidae) Sushil Kumar, Mamta Bhadauria, AKS Chauhan and BS Chandel* Deptt. ... soybean, groundnut, mustard, neem, citrus, peels, Saussurea lappa, coconut and dharek effective against storage, pests. Mahapatra et al. ...

Research paper thumbnail of Histomorphometric study of animal skin exposed to sulphur mustard

Bulletin of Environmental Contamination and Toxicology, 1993

Research paper thumbnail of Nucleotide Sequences and Chromosomal Localization of 45S and 5S rDNA in Neolissochilus hexagonolepis (Pisces, Cyprinidae), Using Dual-Color Fish

Zoological Science, 2010

Dual-color fluorescence in situ hybridization (FISH) was performed to study the simultaneous loca... more Dual-color fluorescence in situ hybridization (FISH) was performed to study the simultaneous localization of major (45S) and minor (5S) family ribosomal RNA genes on chromosomes of Neolissochilus hexagonolepis. The partial 45S (18S, ITS 1, 5.8S, ITS 2 and 28S) and complete 5S (coding and NTS) rDNA units were amplified, sequenced, analyzed, and mapped on the metaphase chromosomes. The complete 18S, 5.8S and partial 28S rDNAs were 1849 bp, 157 bp and 1819 bp long, respectively. Internal transcribed spacers, namely ITS 1 (828 bp) and ITS 2 (359 bp), showed significant nucleotide variations from other fish species listed in NCBI database. The 5S rDNA contained an identical coding region of 120 bp and a highly divergent, non-transcribed 81-bp spacer. The specimens of N. hexagonolepis showed six bright fluorescent signals of 18S, while the 5S signals were present only on one pair of chromosomes. Subsequent analyses between conventional Ag-NORs and 18S rDNA FISH strongly suggested the possible inactivation of one pair of NORs that was localized at a telomeric position of a submetacentric chromosome. The sequencing and chromosomal localization of 45S and 5S rDNAs may serve as a useful genetic marker in taxonomic classification as well as in phylogenetic and evolutionary studies.

Research paper thumbnail of Population distribution of 45S and 5S rDNA in golden mahseer, Tor putitora : population-specific FISH marker

Journal of Genetics, 2009

Chromosomal locations of major 45S and minor 5S ribosomal DNAs (rDNAs) and organization of 5S rRN... more Chromosomal locations of major 45S and minor 5S ribosomal DNAs (rDNAs) and organization of 5S rRNA genes were analysed in five different populations of golden mahseers (Tor putitora) using fluorescence in situ hybridization (FISH) and Southern blot hybridization. All five populations of T. putitora (2n = 100) showed a similar type of macro-karyotype composed of 12 metacentric, 22 submetacentric, 14 subtelocentric and 52 telocentric chromosomes. Analysis of active nucleolar organizer regions (NORs) by silver staining did not show any differences in number and chromosomal position in different populations. But FISH data showed significant difference between the populations, four of the five populations showed six 18S (three pairs) and two 5S (one pair) signals with positional polymorphism, while one population showed eight 18S and four 5S signals, respectively. Southern blot data confirms that 5S rDNA clusters present on two different chromosome pairs in Kosi river population contain non-transcribed spacers (NTS) of same length. In the present study, simultaneous localization of 45S and 5S rDNA by in situ hybridization helped us to develop the discrete population-specific markers in different geographically isolated populations of T. putitora.

Research paper thumbnail of Contribution to Indigenous Drugs Part I: Celosia Argentea

Pharmaceutical Biology, 1993

Page 1. Int. J. Pharmacog., 31 (1993). No. 3, pp. 223-234 0925-16 1819213 103-0223$25 .OO 0 Swets... more Page 1. Int. J. Pharmacog., 31 (1993). No. 3, pp. 223-234 0925-16 1819213 103-0223$25 .OO 0 Swets & Zeitlinger Contribution to Indigenous Drugs Part I: Celosia Argentea* Mamta B. SHAH, KN PATEL and Malati G. CHAUHAN ...

Research paper thumbnail of Use of Certain Naturally Ocurring Herbal Grain Protectants against Sitophilus oryzae Linn. (Coleoptera :Curculionidae

... (Coleoptera :Curculionidae) Sushil Kumar, Mamta Bhadauria, AKS Chauhan and BS Chandel* Deptt.... more ... (Coleoptera :Curculionidae) Sushil Kumar, Mamta Bhadauria, AKS Chauhan and BS Chandel* Deptt. ... soybean, groundnut, mustard, neem, citrus, peels, Saussurea lappa, coconut and dharek effective against storage, pests. Mahapatra et al. ...

Research paper thumbnail of Histomorphometric study of animal skin exposed to sulphur mustard

Bulletin of Environmental Contamination and Toxicology, 1993

Research paper thumbnail of Nucleotide Sequences and Chromosomal Localization of 45S and 5S rDNA in Neolissochilus hexagonolepis (Pisces, Cyprinidae), Using Dual-Color Fish

Zoological Science, 2010

Dual-color fluorescence in situ hybridization (FISH) was performed to study the simultaneous loca... more Dual-color fluorescence in situ hybridization (FISH) was performed to study the simultaneous localization of major (45S) and minor (5S) family ribosomal RNA genes on chromosomes of Neolissochilus hexagonolepis. The partial 45S (18S, ITS 1, 5.8S, ITS 2 and 28S) and complete 5S (coding and NTS) rDNA units were amplified, sequenced, analyzed, and mapped on the metaphase chromosomes. The complete 18S, 5.8S and partial 28S rDNAs were 1849 bp, 157 bp and 1819 bp long, respectively. Internal transcribed spacers, namely ITS 1 (828 bp) and ITS 2 (359 bp), showed significant nucleotide variations from other fish species listed in NCBI database. The 5S rDNA contained an identical coding region of 120 bp and a highly divergent, non-transcribed 81-bp spacer. The specimens of N. hexagonolepis showed six bright fluorescent signals of 18S, while the 5S signals were present only on one pair of chromosomes. Subsequent analyses between conventional Ag-NORs and 18S rDNA FISH strongly suggested the possible inactivation of one pair of NORs that was localized at a telomeric position of a submetacentric chromosome. The sequencing and chromosomal localization of 45S and 5S rDNAs may serve as a useful genetic marker in taxonomic classification as well as in phylogenetic and evolutionary studies.

Research paper thumbnail of Population distribution of 45S and 5S rDNA in golden mahseer, Tor putitora : population-specific FISH marker

Journal of Genetics, 2009

Chromosomal locations of major 45S and minor 5S ribosomal DNAs (rDNAs) and organization of 5S rRN... more Chromosomal locations of major 45S and minor 5S ribosomal DNAs (rDNAs) and organization of 5S rRNA genes were analysed in five different populations of golden mahseers (Tor putitora) using fluorescence in situ hybridization (FISH) and Southern blot hybridization. All five populations of T. putitora (2n = 100) showed a similar type of macro-karyotype composed of 12 metacentric, 22 submetacentric, 14 subtelocentric and 52 telocentric chromosomes. Analysis of active nucleolar organizer regions (NORs) by silver staining did not show any differences in number and chromosomal position in different populations. But FISH data showed significant difference between the populations, four of the five populations showed six 18S (three pairs) and two 5S (one pair) signals with positional polymorphism, while one population showed eight 18S and four 5S signals, respectively. Southern blot data confirms that 5S rDNA clusters present on two different chromosome pairs in Kosi river population contain non-transcribed spacers (NTS) of same length. In the present study, simultaneous localization of 45S and 5S rDNA by in situ hybridization helped us to develop the discrete population-specific markers in different geographically isolated populations of T. putitora.

Research paper thumbnail of Contribution to Indigenous Drugs Part I: Celosia Argentea

Pharmaceutical Biology, 1993

Page 1. Int. J. Pharmacog., 31 (1993). No. 3, pp. 223-234 0925-16 1819213 103-0223$25 .OO 0 Swets... more Page 1. Int. J. Pharmacog., 31 (1993). No. 3, pp. 223-234 0925-16 1819213 103-0223$25 .OO 0 Swets & Zeitlinger Contribution to Indigenous Drugs Part I: Celosia Argentea* Mamta B. SHAH, KN PATEL and Malati G. CHAUHAN ...