Krystyna Naumenko | Danilo Zabolotny Institute of Microbiology and Virology National Academy Scieces of Ukraine (original) (raw)
Papers by Krystyna Naumenko
Condensed Matter
Nanosized composites CeO2–Ag, La2O3–Ag, and TiO2–Ag are a class of nanomaterials suitable for pho... more Nanosized composites CeO2–Ag, La2O3–Ag, and TiO2–Ag are a class of nanomaterials suitable for photocatalysis, optical devices, and photoelectrochemical elements. Further, nanocomposites with several wt.% of silver can be used as creating materials for pathogenic virus inactivation with pandemic-neutralizing potential. Thus, CeO2–Ag, La2O3–Ag, and TiO2–Ag nanocomposites are prospective materials due to their optical and biological activity. In the present work, CeO2–Ag, La2O3–Ag, and TiO2–Ag nanocomposites were synthesized by the co-precipitation method. The morphological and optical properties and the structure of the prepared nanocomposites were analyzed using X-ray diffraction (XRD), scanning electron microscopy (SEM) with EDX, and nitrogen adsorption-desorption based on BET, Raman spectroscopy, and photoluminescence (PL). Both oxide matrixes corresponded to the cubic crystal lattice with the inclusion of argentum into the crystal lattice of oxides at relative low c(Ag) and reduct...
Molecules
Green synthesis of silver-containing nanocomposites based on polylactide (PLA) was carried out in... more Green synthesis of silver-containing nanocomposites based on polylactide (PLA) was carried out in two ways. With the use of green tea extract, Ag+ ions were reduced to silver nanoparticles with their subsequent introduction into the PLA (mechanical method) and Ag+ ions were reduced in the polymer matrix of PLA-AgPalmitate (PLA-AgPalm) (in situ method). Structure, morphology and thermophysical properties of nanocomposites PLA-Ag were studied by FTIR spectroscopy, wide-angle X-ray scattering (WAXS), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) methods. The antimicrobial, antiviral, and cytotoxic properties were studied as well. It was found that the mechanical method provides the average size of silver nanoparticles in the PLA of about 16 nm, while in the formation of samples by the in situ method their average size was 3.7 nm. The strong influence of smaller silver nanoparticles (3.7 nm) on the properties of nan...
Viral co-infection is one of the current and unexplored issues of human infectious diseases. A sp... more Viral co-infection is one of the current and unexplored issues of human infectious diseases. A special place in the progression of these pathologies is occupied by adeno-and herpes viruses being able to long persist in the body. There is a huge lack of knowledge about antiviral activities of specific drugs during the mixed infections. For the testing of the antiviral medication acyclovir (ACV) and for research of new fluorine-containing analog of L-phenylalanine (10S-24), the model of simultaneous adeno-herpetic infection of MDBK cells was used. Determination of the antiviral effect using cytomorphology method, infectious virus yield reduction assay and cell cycle analysis demonstrated the inhibitory effect of the 10S24 on the late phase of the HSV-1 and HAdV5 reproduction. Decreased activity of the substance was resulted by 16-57% compared to HSV-1 through the application of 10S-24 at mixed infection. The use of the ACV at mixed infection, reduces its activity against HSV-1 by 16-4...
Over the past 10 years, many scientific groups have experimentally shown that non-functionalized ... more Over the past 10 years, many scientific groups have experimentally shown that non-functionalized nanoparticles show a pronounced antiviral and antimicrobial action against different pathogens. In order to understand the mechanism of nanoparticles action it is important to know its peculiarities, i.e. dependences on different nanoparticles and pathogen properties.In this work we studied how Au nanoparticles act on the viruses outside and inside the cell, and compare this action for two sizes of nanoparticles and two types of the viruses. The study has been conducted for adenovirus and H1N1 influenza virus, and nanoparticles of 5 nm and 20 nm diameter.Virucidal and antiviral actions were observed experimentally for both types of nanoparticles against both viruses. It has been shown that intensity of virucidal action depends on the nanoparticles concentration non-monotonically for adenovirus. It has also been shown with electron microscopy that the viruses are destructed after 5 nm nan...
Proceedings of 7th International Electronic Conference on Medicinal Chemistry
On a global scale, Epstein-Barr virus (EBV) infects over 90% of the adult population and is respo... more On a global scale, Epstein-Barr virus (EBV) infects over 90% of the adult population and is responsible for ~1% of all human cancers. Fluorine is one of the most abundant elements on earth. However, it occurs extremely rarely in biological compounds. The introduction of the fluorine atom(s) into many biologically active molecules can bring about remarkable and profound changes in their properties. Development of potential drugs is closely related to in silico methods, which include PASS, QSAR, COMPARE-analysis and more. The aim of this work was to analyze the potential biological activity and the target of action of derivatives of bisphosphonic acids by using in silico methods and examined received results by in vitro study. For this purpose, PASS software, web-server PharmMapper, PCR, MTT assay, trypan blue and neutral red assay were used. According to PASS prediction two compounds (10s-20 and 10s-21) may possess antiviral activity, Pa/ Pi was 0,294/0,005 and 0,214/0,084, respectiv...
Mixed viral infection is one of the current and unexplored issues of human infectious diseases. A... more Mixed viral infection is one of the current and unexplored issues of human infectious diseases. A special place in the development of these pathologies is occupied by adeno- and herpes viruses that are able to persist for a long time in a latent condition in the body. There is a huge lack of knowledge about antiviral activities of specific drugs during the mixed infections. The study of know drugs and discovery of new compounds using not only standard mono-infections but also with created mixed infections is a topical and a new direction in antivirus screening. Previously in our department, the models of adeno-herpetic infections in cells of different origins were created and the features of the development of viral infections in these systems were studied. The model of simultaneous adeno-herpetic infection of MDBK cells was used for the analysis of the antiherpetic drug acyclovir (ACV) and for research of new fluorine-containing derivatives of L-phenylalanine (10S-23 and 10S-2...
The Epstein-Barr virus (EBV) is the first virus that has been classified as a human oncology viru... more The Epstein-Barr virus (EBV) is the first virus that has been classified as a human oncology virus. The ability of EBV to immortalize the cells of the human body is the highest among all known transforming viruses. Fluorine-containing nucleoside analogs represent a significant class of the chemotherapeutics widely used in the treatment for a lot of diseases. They have been playing a major role in treating tumor and virus either as selective inhibitors of enzymes for cancer or viral replication or as nucleic acid chain terminators which interrupt the replication of cancer cells or a virus. Aim. The purpose of this study was to analyze the potential antiviral and apoptosis modulating activity of fluorinated derivatives of uracil by using in silico and in vitro methods. Materials and methods. Two analogs (compound G26 and G27) on the base of 5-(p-tolilsulfonil)-6(polyfluoroalkyl)uracil were used in the study. The studies were conducted on cultures of Raji (latent infected EBV) and B95-...
JOURNAL OF ADVANCES IN BIOLOGY, 2016
The research of the molecular and genetic characteristics of Epstein-Barr virus are going for yea... more The research of the molecular and genetic characteristics of Epstein-Barr virus are going for years and its ability to develop lymphoproliferative diseases. However, the search for effective antiviral and anticancer drugs remains relevant today. The aim was to investigate the biological properties methylenebisphosphonic acids (10s20-22), including cytotoxicity, apoptosis stimulating and antiviral activity. For this we used MTT-metod, PCR and flow cytometry. Also bioinformatic analysis was conducted using compounds PASS. It was shown 10s-20 compound effectively inhibits the replication of EBV in low concentrations. Compound 10c-21 showed a significant effect on apoptosis stimulating B95-8 cells. Data analysis showed prediction that this class of compounds may be a substrate for cytochrome C (CYP2H), which in turn may indicate the involvement of mitochondrial ways of inducing apoptosis. The data indicate that the group methylenebisphosphonic acids are perspective for further research
Mikrobiolohichnyi Zhurnal
Mikrobiolohichnyi Zhurnal
ScienceRise: Biological Science, Feb 27, 2018
Першим вірусом класифікованим, як онкологічний був вірус Епштейна-Барр. Метою роботи було досліди... more Першим вірусом класифікованим, як онкологічний був вірус Епштейна-Барр. Метою роботи було дослідити біологічні властивості фторованих похідних на основі урацилу. Згідно прогнозування PASS, сполуки G26 та G27 можуть мати противірусну активність. Анти-ВЕБ активність in vitro була показана для обох досліджуваних сполук. Таким чином, похідні урацилу є перспективними для створення нових антивірусних агентів Ключові слова: вірус Епштейна-Барр, фторовані аналоги нуклеозидів, апоптоз, in silico та in vitro
Mikrobiolohichnyi Zhurnal
Pharmaceuticals (Basel, Switzerland), Jan 31, 2017
The second International Electronic Conference on Medicinal Chemistry, organized and sponsored by... more The second International Electronic Conference on Medicinal Chemistry, organized and sponsored by the publisher MDPI AG and the Journal Pharmaceuticals, took place in November 2016 on the SciForum website (www.sciforum.net/conference/ecmc-12). More than 150 authors from 22 countries participated in the event. Selected works presented during the scientific meeting are disclosed in this report.
Reports of the National Academy of Sciences of Ukraine, 2015
Microbiology&Biotechnology, Dec 28, 2017
The purpose of this work was to analyze the potential biological activity and the target of actio... more The purpose of this work was to analyze the potential biological activity and the target of action of esters of tetrafluoropropanethioacylated amino acids derivatives and to investigate the obtained results in vitro. Materials and methods. PASS software, web-server PharmMapper, PCR, MTT assay, trypan blue and neutral red assay were used. Results. According to PASS prediction, two compounds (10S20 and 10S21) may possess antiviral activity, Pa/ Pi was 0,294/0,005 and 0,214/0,084, respectively. Also, all compounds may possess a cytochrome c as the substrates that might play an important role in the induction of apoptosis. Several targets were identified by using molecular docking (PharmMapper). It was shown that a lot of possible targets are proteins, such as Gag-Pol protein (viral protein) and different kinds of protein kinases. Study in vitro showed that all compounds inhibited the replication of the Epstein-Barr virus. On the other hand, esters of fluorinated aminoacids have high levels of cytotoxicity in various lymphoblastoid cell lines, which may be due to the mechanism of action of the compounds. Conclusions. Our results let to relate the compound tert-butyl (2,2,3,3-tetrafluor opropanethioyl)alaniate to a perspective anti-EBV agent. Analyzed data showed, that polyfluoroalkanethioacylated amino acids derivatives (10S21 and 10S22) may possess apoptosis modulating properties.
Reactive and Functional Polymers
Proceedings of 5th International Electronic Conference on Medicinal Chemistry
Viral infections occupy an important place in human infectious pathology, as one of the most comm... more Viral infections occupy an important place in human infectious pathology, as one of the most common and poorly controlled infections. At present, Influenza A virus (IAV) and Epstein-Barr virus (EBV) cause different infections, thereby imposing a huge toll on both human health and the economy worldwide. Therefore, screening new effective drugs is an urgent and important problem. Our study aimed to determine cytotoxicity and antiviral effect of fluorinated compounds against the Influenza A virus and Epstein-Barr virus. The cytotoxicity was studied in two cell lines by MTT assay. The cytotoxicity of the compounds the rate was 41-990 μg/ml in the B95-8 cell culture, which produces EBV, and 200-478 μg/ml in MDCK cells. Compounds 10S-47 and 10S-49 were able to inhibit reproduction of IAV with EC 50 of 38 μg/ml and 50 μg/ml, respectively. It was shown that fluorinated compounds showed low effectiveness against the Epstein-Barr virus, and inhibited the reproduction of the virus at 20% in the concentration range of 1-100 μg/ml. Also, we studied the potency of compound 10S-47 to make an apoptosis induction because it exhibited a significant cytotoxic effect on the growth of transformed cells. It was established that for compound 10S-47 at 40 mg/ml, the percentage of apoptotic cells exceeded the control and was 10%.
JOURNAL OF ADVANCES IN BIOLOGY
In the present work, it was shown that, in non-toxic concentrations, all EPSs which were isolated... more In the present work, it was shown that, in non-toxic concentrations, all EPSs which were isolated from lactic acid bacteria have significant anti-HSV-1 activity affecting various stages of the virus reproduction. It was demonstrated that the use of ESPs leads to the normalization of the life cycle of cells infected with the herpes simplex virus to the level of uninfected cells. It was found that EPS 26a produced by Lactobacillus sp. possesses multiple antiviral effects as it exhibits virucidal activity, blocks adsorption, penetration of the virus into cells and the release of viruses, and reduces the infectious titer of herpes simplex virus by 97-99%, indicating its considerable anti-herpetic activity.
Proceedings of 4th International Electronic Conference on Medicinal Chemistry
Epstein-Barr virus (EBV) causes lymphocyte-proliferative diseases, such as Burkitt's lymphoma, Ho... more Epstein-Barr virus (EBV) causes lymphocyte-proliferative diseases, such as Burkitt's lymphoma, Hodgkin's lymphoma, other B and T cell lymphomas. Recently the connection between EBV and autoimmunity diseases has been demonstrated. In recent years, several studies have explored the concept that the compounds that have antiherpetic activity might be able to influence the cell cycle of infected cells, by eliminating them from the body. However, cell cycle regulation during EBV-infection and the effect of anti-EBV drugs have received only limited attention. The aim of our work was to study derivatives of triazole (G14, G20, G22, and G24) as potential antiherpetic agents and their effect on the cell cycle of lymphoblastoid cell lines B95-8. According to PCR, anti-EBV activity was observed only for compounds G14 and G22, EC50 values were 27 and 100µg/ml. The В95-8 cells treated with all studied compounds were analyzed with the help of flow cytometry (cells were stained with propidium iodide). It was observed an induction of apoptosis in the presence of G22 at 700µg/ml; the proportion of apoptotic cells reached almost 40%. Other compounds G14 and G24 led to the switch of cells from the Sub G0 phase of the cell cycle to the G1 phase and subsequent activation of the S-phase. These compounds may play an important role as potential inducers of EBV lytic infection; with the addition of antiherpesvirus drugs, they could be therapeutically beneficial for EBVassociated tumors.
Condensed Matter
Nanosized composites CeO2–Ag, La2O3–Ag, and TiO2–Ag are a class of nanomaterials suitable for pho... more Nanosized composites CeO2–Ag, La2O3–Ag, and TiO2–Ag are a class of nanomaterials suitable for photocatalysis, optical devices, and photoelectrochemical elements. Further, nanocomposites with several wt.% of silver can be used as creating materials for pathogenic virus inactivation with pandemic-neutralizing potential. Thus, CeO2–Ag, La2O3–Ag, and TiO2–Ag nanocomposites are prospective materials due to their optical and biological activity. In the present work, CeO2–Ag, La2O3–Ag, and TiO2–Ag nanocomposites were synthesized by the co-precipitation method. The morphological and optical properties and the structure of the prepared nanocomposites were analyzed using X-ray diffraction (XRD), scanning electron microscopy (SEM) with EDX, and nitrogen adsorption-desorption based on BET, Raman spectroscopy, and photoluminescence (PL). Both oxide matrixes corresponded to the cubic crystal lattice with the inclusion of argentum into the crystal lattice of oxides at relative low c(Ag) and reduct...
Molecules
Green synthesis of silver-containing nanocomposites based on polylactide (PLA) was carried out in... more Green synthesis of silver-containing nanocomposites based on polylactide (PLA) was carried out in two ways. With the use of green tea extract, Ag+ ions were reduced to silver nanoparticles with their subsequent introduction into the PLA (mechanical method) and Ag+ ions were reduced in the polymer matrix of PLA-AgPalmitate (PLA-AgPalm) (in situ method). Structure, morphology and thermophysical properties of nanocomposites PLA-Ag were studied by FTIR spectroscopy, wide-angle X-ray scattering (WAXS), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) methods. The antimicrobial, antiviral, and cytotoxic properties were studied as well. It was found that the mechanical method provides the average size of silver nanoparticles in the PLA of about 16 nm, while in the formation of samples by the in situ method their average size was 3.7 nm. The strong influence of smaller silver nanoparticles (3.7 nm) on the properties of nan...
Viral co-infection is one of the current and unexplored issues of human infectious diseases. A sp... more Viral co-infection is one of the current and unexplored issues of human infectious diseases. A special place in the progression of these pathologies is occupied by adeno-and herpes viruses being able to long persist in the body. There is a huge lack of knowledge about antiviral activities of specific drugs during the mixed infections. For the testing of the antiviral medication acyclovir (ACV) and for research of new fluorine-containing analog of L-phenylalanine (10S-24), the model of simultaneous adeno-herpetic infection of MDBK cells was used. Determination of the antiviral effect using cytomorphology method, infectious virus yield reduction assay and cell cycle analysis demonstrated the inhibitory effect of the 10S24 on the late phase of the HSV-1 and HAdV5 reproduction. Decreased activity of the substance was resulted by 16-57% compared to HSV-1 through the application of 10S-24 at mixed infection. The use of the ACV at mixed infection, reduces its activity against HSV-1 by 16-4...
Over the past 10 years, many scientific groups have experimentally shown that non-functionalized ... more Over the past 10 years, many scientific groups have experimentally shown that non-functionalized nanoparticles show a pronounced antiviral and antimicrobial action against different pathogens. In order to understand the mechanism of nanoparticles action it is important to know its peculiarities, i.e. dependences on different nanoparticles and pathogen properties.In this work we studied how Au nanoparticles act on the viruses outside and inside the cell, and compare this action for two sizes of nanoparticles and two types of the viruses. The study has been conducted for adenovirus and H1N1 influenza virus, and nanoparticles of 5 nm and 20 nm diameter.Virucidal and antiviral actions were observed experimentally for both types of nanoparticles against both viruses. It has been shown that intensity of virucidal action depends on the nanoparticles concentration non-monotonically for adenovirus. It has also been shown with electron microscopy that the viruses are destructed after 5 nm nan...
Proceedings of 7th International Electronic Conference on Medicinal Chemistry
On a global scale, Epstein-Barr virus (EBV) infects over 90% of the adult population and is respo... more On a global scale, Epstein-Barr virus (EBV) infects over 90% of the adult population and is responsible for ~1% of all human cancers. Fluorine is one of the most abundant elements on earth. However, it occurs extremely rarely in biological compounds. The introduction of the fluorine atom(s) into many biologically active molecules can bring about remarkable and profound changes in their properties. Development of potential drugs is closely related to in silico methods, which include PASS, QSAR, COMPARE-analysis and more. The aim of this work was to analyze the potential biological activity and the target of action of derivatives of bisphosphonic acids by using in silico methods and examined received results by in vitro study. For this purpose, PASS software, web-server PharmMapper, PCR, MTT assay, trypan blue and neutral red assay were used. According to PASS prediction two compounds (10s-20 and 10s-21) may possess antiviral activity, Pa/ Pi was 0,294/0,005 and 0,214/0,084, respectiv...
Mixed viral infection is one of the current and unexplored issues of human infectious diseases. A... more Mixed viral infection is one of the current and unexplored issues of human infectious diseases. A special place in the development of these pathologies is occupied by adeno- and herpes viruses that are able to persist for a long time in a latent condition in the body. There is a huge lack of knowledge about antiviral activities of specific drugs during the mixed infections. The study of know drugs and discovery of new compounds using not only standard mono-infections but also with created mixed infections is a topical and a new direction in antivirus screening. Previously in our department, the models of adeno-herpetic infections in cells of different origins were created and the features of the development of viral infections in these systems were studied. The model of simultaneous adeno-herpetic infection of MDBK cells was used for the analysis of the antiherpetic drug acyclovir (ACV) and for research of new fluorine-containing derivatives of L-phenylalanine (10S-23 and 10S-2...
The Epstein-Barr virus (EBV) is the first virus that has been classified as a human oncology viru... more The Epstein-Barr virus (EBV) is the first virus that has been classified as a human oncology virus. The ability of EBV to immortalize the cells of the human body is the highest among all known transforming viruses. Fluorine-containing nucleoside analogs represent a significant class of the chemotherapeutics widely used in the treatment for a lot of diseases. They have been playing a major role in treating tumor and virus either as selective inhibitors of enzymes for cancer or viral replication or as nucleic acid chain terminators which interrupt the replication of cancer cells or a virus. Aim. The purpose of this study was to analyze the potential antiviral and apoptosis modulating activity of fluorinated derivatives of uracil by using in silico and in vitro methods. Materials and methods. Two analogs (compound G26 and G27) on the base of 5-(p-tolilsulfonil)-6(polyfluoroalkyl)uracil were used in the study. The studies were conducted on cultures of Raji (latent infected EBV) and B95-...
JOURNAL OF ADVANCES IN BIOLOGY, 2016
The research of the molecular and genetic characteristics of Epstein-Barr virus are going for yea... more The research of the molecular and genetic characteristics of Epstein-Barr virus are going for years and its ability to develop lymphoproliferative diseases. However, the search for effective antiviral and anticancer drugs remains relevant today. The aim was to investigate the biological properties methylenebisphosphonic acids (10s20-22), including cytotoxicity, apoptosis stimulating and antiviral activity. For this we used MTT-metod, PCR and flow cytometry. Also bioinformatic analysis was conducted using compounds PASS. It was shown 10s-20 compound effectively inhibits the replication of EBV in low concentrations. Compound 10c-21 showed a significant effect on apoptosis stimulating B95-8 cells. Data analysis showed prediction that this class of compounds may be a substrate for cytochrome C (CYP2H), which in turn may indicate the involvement of mitochondrial ways of inducing apoptosis. The data indicate that the group methylenebisphosphonic acids are perspective for further research
Mikrobiolohichnyi Zhurnal
Mikrobiolohichnyi Zhurnal
ScienceRise: Biological Science, Feb 27, 2018
Першим вірусом класифікованим, як онкологічний був вірус Епштейна-Барр. Метою роботи було досліди... more Першим вірусом класифікованим, як онкологічний був вірус Епштейна-Барр. Метою роботи було дослідити біологічні властивості фторованих похідних на основі урацилу. Згідно прогнозування PASS, сполуки G26 та G27 можуть мати противірусну активність. Анти-ВЕБ активність in vitro була показана для обох досліджуваних сполук. Таким чином, похідні урацилу є перспективними для створення нових антивірусних агентів Ключові слова: вірус Епштейна-Барр, фторовані аналоги нуклеозидів, апоптоз, in silico та in vitro
Mikrobiolohichnyi Zhurnal
Pharmaceuticals (Basel, Switzerland), Jan 31, 2017
The second International Electronic Conference on Medicinal Chemistry, organized and sponsored by... more The second International Electronic Conference on Medicinal Chemistry, organized and sponsored by the publisher MDPI AG and the Journal Pharmaceuticals, took place in November 2016 on the SciForum website (www.sciforum.net/conference/ecmc-12). More than 150 authors from 22 countries participated in the event. Selected works presented during the scientific meeting are disclosed in this report.
Reports of the National Academy of Sciences of Ukraine, 2015
Microbiology&Biotechnology, Dec 28, 2017
The purpose of this work was to analyze the potential biological activity and the target of actio... more The purpose of this work was to analyze the potential biological activity and the target of action of esters of tetrafluoropropanethioacylated amino acids derivatives and to investigate the obtained results in vitro. Materials and methods. PASS software, web-server PharmMapper, PCR, MTT assay, trypan blue and neutral red assay were used. Results. According to PASS prediction, two compounds (10S20 and 10S21) may possess antiviral activity, Pa/ Pi was 0,294/0,005 and 0,214/0,084, respectively. Also, all compounds may possess a cytochrome c as the substrates that might play an important role in the induction of apoptosis. Several targets were identified by using molecular docking (PharmMapper). It was shown that a lot of possible targets are proteins, such as Gag-Pol protein (viral protein) and different kinds of protein kinases. Study in vitro showed that all compounds inhibited the replication of the Epstein-Barr virus. On the other hand, esters of fluorinated aminoacids have high levels of cytotoxicity in various lymphoblastoid cell lines, which may be due to the mechanism of action of the compounds. Conclusions. Our results let to relate the compound tert-butyl (2,2,3,3-tetrafluor opropanethioyl)alaniate to a perspective anti-EBV agent. Analyzed data showed, that polyfluoroalkanethioacylated amino acids derivatives (10S21 and 10S22) may possess apoptosis modulating properties.
Reactive and Functional Polymers
Proceedings of 5th International Electronic Conference on Medicinal Chemistry
Viral infections occupy an important place in human infectious pathology, as one of the most comm... more Viral infections occupy an important place in human infectious pathology, as one of the most common and poorly controlled infections. At present, Influenza A virus (IAV) and Epstein-Barr virus (EBV) cause different infections, thereby imposing a huge toll on both human health and the economy worldwide. Therefore, screening new effective drugs is an urgent and important problem. Our study aimed to determine cytotoxicity and antiviral effect of fluorinated compounds against the Influenza A virus and Epstein-Barr virus. The cytotoxicity was studied in two cell lines by MTT assay. The cytotoxicity of the compounds the rate was 41-990 μg/ml in the B95-8 cell culture, which produces EBV, and 200-478 μg/ml in MDCK cells. Compounds 10S-47 and 10S-49 were able to inhibit reproduction of IAV with EC 50 of 38 μg/ml and 50 μg/ml, respectively. It was shown that fluorinated compounds showed low effectiveness against the Epstein-Barr virus, and inhibited the reproduction of the virus at 20% in the concentration range of 1-100 μg/ml. Also, we studied the potency of compound 10S-47 to make an apoptosis induction because it exhibited a significant cytotoxic effect on the growth of transformed cells. It was established that for compound 10S-47 at 40 mg/ml, the percentage of apoptotic cells exceeded the control and was 10%.
JOURNAL OF ADVANCES IN BIOLOGY
In the present work, it was shown that, in non-toxic concentrations, all EPSs which were isolated... more In the present work, it was shown that, in non-toxic concentrations, all EPSs which were isolated from lactic acid bacteria have significant anti-HSV-1 activity affecting various stages of the virus reproduction. It was demonstrated that the use of ESPs leads to the normalization of the life cycle of cells infected with the herpes simplex virus to the level of uninfected cells. It was found that EPS 26a produced by Lactobacillus sp. possesses multiple antiviral effects as it exhibits virucidal activity, blocks adsorption, penetration of the virus into cells and the release of viruses, and reduces the infectious titer of herpes simplex virus by 97-99%, indicating its considerable anti-herpetic activity.
Proceedings of 4th International Electronic Conference on Medicinal Chemistry
Epstein-Barr virus (EBV) causes lymphocyte-proliferative diseases, such as Burkitt's lymphoma, Ho... more Epstein-Barr virus (EBV) causes lymphocyte-proliferative diseases, such as Burkitt's lymphoma, Hodgkin's lymphoma, other B and T cell lymphomas. Recently the connection between EBV and autoimmunity diseases has been demonstrated. In recent years, several studies have explored the concept that the compounds that have antiherpetic activity might be able to influence the cell cycle of infected cells, by eliminating them from the body. However, cell cycle regulation during EBV-infection and the effect of anti-EBV drugs have received only limited attention. The aim of our work was to study derivatives of triazole (G14, G20, G22, and G24) as potential antiherpetic agents and their effect on the cell cycle of lymphoblastoid cell lines B95-8. According to PCR, anti-EBV activity was observed only for compounds G14 and G22, EC50 values were 27 and 100µg/ml. The В95-8 cells treated with all studied compounds were analyzed with the help of flow cytometry (cells were stained with propidium iodide). It was observed an induction of apoptosis in the presence of G22 at 700µg/ml; the proportion of apoptotic cells reached almost 40%. Other compounds G14 and G24 led to the switch of cells from the Sub G0 phase of the cell cycle to the G1 phase and subsequent activation of the S-phase. These compounds may play an important role as potential inducers of EBV lytic infection; with the addition of antiherpesvirus drugs, they could be therapeutically beneficial for EBVassociated tumors.