Ahmed Osman | INCEIF (Intern. Centre for Edu. in Islamic Finance) (original) (raw)
Papers by Ahmed Osman
Experimental Parasitology, 2007
Infection and Immunity, 2000
Molecular and Biochemical Parasitology, 1999
Molecular and Biochemical Parasitology, 2001
Recently, we reported the identification of cDNA's encoding retinoid X receptor (... more Recently, we reported the identification of cDNA's encoding retinoid X receptor (RXR) homologues in Schistosoma mansoni. RXRs are known to be involved in the regulation of genes important for homeostasis and development. Previous studies indicated that SmRXR1 plays a role in the regulation of the female-specific gene, p14. Herein, we report that SmRXR2 also binds to cis-elements present in the p14 upstream region when evaluated in yeast reporter strains. SmRXR2 shows a pattern of recognition of cis-sequences present in the p14 gene upstream region different than SmRXR1. However, the SmRXR2 C (DNA binding) domain binds promiscuously in electrophoretic mobility shift assays to cis-elements of the p14 upstream region. The SmRXRs differ in their ability to activate transcription. The N-terminal A/B domain of SmRXR1 is necessary and sufficient for autonomous transcription activation function (AF) in yeast. SmRXR2 does not exhibit an equivalent autonomous AF. SmRXR1 and SmRXR2 fail to dimerize when investigated both in the yeast two-hybrid system and in immunoprecipitation experiments. In situ hybridization experiments using paraffin sections of adult worms demonstrate that SmRXR1 and SmRXR2 exhibit both common and unique cell type distribution which indicates that SmRXR1 and SmRXR2 both play a role in regulating gene expression in certain cells, yet each plays a distinct role in modulating the expression of genes in other cell types. Both SmRXR1 and SmRXR2 localize to vitelline cells. These studies provide a solid basis for improving our understanding of RXRs and their importance in female-specific gene regulation.
Canadian Journal of Zoology-revue Canadienne De Zoologie, 2004
Abstract: Schistosome parasites are muticellular eucaryotic organisms with a complex life cycle t... more Abstract: Schistosome parasites are muticellular eucaryotic organisms with a complex life cycle that involves mamma-lian and snail hosts. Unlike other trematode parasites, schistosomes (along with the Didymozoidae) have evolved sepa-rate sexes or dioecy. Sex is determined by a ...
Journal of Parasitology, 2003
Expressed sequence tag (EST) sequencing and analysis is a primary research tool to identify and c... more Expressed sequence tag (EST) sequencing and analysis is a primary research tool to identify and characterize the Schistosoma mansoni transcriptome. As part of our gene discovery effort, a total of 5,793 ESTs have been generated from clones selected randomly from complementary DNA (cDNA) libraries constructed from male and female adult worms. Assembly analysis of all the 16,813 public S. mansoni ESTs has identified 1,920 distinct tentative consensus sequences (TCs) and 5,571 nonoverlapping ESTs (singletons). Of these, 376 TCs (20%) and 1,449 singletons (26%) are unique to the SUNY/TIGR sequencing effort. Tentative consensus sequences and singletons were distributed into various categories of biological roles associated with cell structure, metabolism, protein fate, signal transduction, transcription, protein synthesis, transporters, and cell growth. The TCs and singletons represent transcripts that can be used as a resource for functional annotation of genomic sequence data, comparative sequence analysis, and cDNA clone selection for microarray projects. The utility of EST analysis is demonstrated by identifying new protease genes, which may be involved in hemoglobin degradation.
Molecular and Biochemical Parasitology, 2004
Measurement Science & Technology, 2004
The last two decades have shown an increasing trend in the use of navigation technologies in seve... more The last two decades have shown an increasing trend in the use of navigation technologies in several applications including land vehicles and automated car navigation. Navigation systems incorporate the global positioning system (GPS) and the inertial navigation system (INS). While GPS provides position information when there is direct line of sight to four or more satellites, INS utilizes the local measurements of angular velocity and linear acceleration to determine both the vehicle's position and attitude. Both systems are integrated together to provide reliable navigation solutions by overcoming each of their respective shortcomings. The present integration schemes, which are predominantly based on Kalman filtering, have several inadequacies related to sensor error models, immunity to noise and observability. This paper aims at introducing a multi-sensor system integration approach for fusing data from an INS and GPS hardware utilizing wavelet multi-resolution analysis (WMRA) and artificial neural networks (ANN). The WMRA is used to compare the INS and GPS position outputs at different resolution levels. The ANN module is then trained to predict the INS position errors in real time and provide accurate positioning of the moving vehicle. The field-test results have demonstrated that substantial improvements in INS/GPS positioning accuracy could be obtained by applying the proposed neuro-wavelet technique.
A. INSiGPS integration Global positioning systems (GPS) are capable of providing accurate positio... more A. INSiGPS integration Global positioning systems (GPS) are capable of providing accurate position infomation with respect to a navigation frame. The three position components are along the East direction (corresponding to the vehicle longitude h), the North direction (corresponding to ...
Experimental Parasitology, 2007
Infection and Immunity, 2000
Molecular and Biochemical Parasitology, 1999
Molecular and Biochemical Parasitology, 2001
Recently, we reported the identification of cDNA's encoding retinoid X receptor (... more Recently, we reported the identification of cDNA's encoding retinoid X receptor (RXR) homologues in Schistosoma mansoni. RXRs are known to be involved in the regulation of genes important for homeostasis and development. Previous studies indicated that SmRXR1 plays a role in the regulation of the female-specific gene, p14. Herein, we report that SmRXR2 also binds to cis-elements present in the p14 upstream region when evaluated in yeast reporter strains. SmRXR2 shows a pattern of recognition of cis-sequences present in the p14 gene upstream region different than SmRXR1. However, the SmRXR2 C (DNA binding) domain binds promiscuously in electrophoretic mobility shift assays to cis-elements of the p14 upstream region. The SmRXRs differ in their ability to activate transcription. The N-terminal A/B domain of SmRXR1 is necessary and sufficient for autonomous transcription activation function (AF) in yeast. SmRXR2 does not exhibit an equivalent autonomous AF. SmRXR1 and SmRXR2 fail to dimerize when investigated both in the yeast two-hybrid system and in immunoprecipitation experiments. In situ hybridization experiments using paraffin sections of adult worms demonstrate that SmRXR1 and SmRXR2 exhibit both common and unique cell type distribution which indicates that SmRXR1 and SmRXR2 both play a role in regulating gene expression in certain cells, yet each plays a distinct role in modulating the expression of genes in other cell types. Both SmRXR1 and SmRXR2 localize to vitelline cells. These studies provide a solid basis for improving our understanding of RXRs and their importance in female-specific gene regulation.
Canadian Journal of Zoology-revue Canadienne De Zoologie, 2004
Abstract: Schistosome parasites are muticellular eucaryotic organisms with a complex life cycle t... more Abstract: Schistosome parasites are muticellular eucaryotic organisms with a complex life cycle that involves mamma-lian and snail hosts. Unlike other trematode parasites, schistosomes (along with the Didymozoidae) have evolved sepa-rate sexes or dioecy. Sex is determined by a ...
Journal of Parasitology, 2003
Expressed sequence tag (EST) sequencing and analysis is a primary research tool to identify and c... more Expressed sequence tag (EST) sequencing and analysis is a primary research tool to identify and characterize the Schistosoma mansoni transcriptome. As part of our gene discovery effort, a total of 5,793 ESTs have been generated from clones selected randomly from complementary DNA (cDNA) libraries constructed from male and female adult worms. Assembly analysis of all the 16,813 public S. mansoni ESTs has identified 1,920 distinct tentative consensus sequences (TCs) and 5,571 nonoverlapping ESTs (singletons). Of these, 376 TCs (20%) and 1,449 singletons (26%) are unique to the SUNY/TIGR sequencing effort. Tentative consensus sequences and singletons were distributed into various categories of biological roles associated with cell structure, metabolism, protein fate, signal transduction, transcription, protein synthesis, transporters, and cell growth. The TCs and singletons represent transcripts that can be used as a resource for functional annotation of genomic sequence data, comparative sequence analysis, and cDNA clone selection for microarray projects. The utility of EST analysis is demonstrated by identifying new protease genes, which may be involved in hemoglobin degradation.
Molecular and Biochemical Parasitology, 2004
Measurement Science & Technology, 2004
The last two decades have shown an increasing trend in the use of navigation technologies in seve... more The last two decades have shown an increasing trend in the use of navigation technologies in several applications including land vehicles and automated car navigation. Navigation systems incorporate the global positioning system (GPS) and the inertial navigation system (INS). While GPS provides position information when there is direct line of sight to four or more satellites, INS utilizes the local measurements of angular velocity and linear acceleration to determine both the vehicle's position and attitude. Both systems are integrated together to provide reliable navigation solutions by overcoming each of their respective shortcomings. The present integration schemes, which are predominantly based on Kalman filtering, have several inadequacies related to sensor error models, immunity to noise and observability. This paper aims at introducing a multi-sensor system integration approach for fusing data from an INS and GPS hardware utilizing wavelet multi-resolution analysis (WMRA) and artificial neural networks (ANN). The WMRA is used to compare the INS and GPS position outputs at different resolution levels. The ANN module is then trained to predict the INS position errors in real time and provide accurate positioning of the moving vehicle. The field-test results have demonstrated that substantial improvements in INS/GPS positioning accuracy could be obtained by applying the proposed neuro-wavelet technique.
A. INSiGPS integration Global positioning systems (GPS) are capable of providing accurate positio... more A. INSiGPS integration Global positioning systems (GPS) are capable of providing accurate position infomation with respect to a navigation frame. The three position components are along the East direction (corresponding to the vehicle longitude h), the North direction (corresponding to ...