Ökkeş ATICI - Academia.edu (original) (raw)
Papers by Ökkeş ATICI
Nepeta transcaucasica Grossh.’dan ekstrakte edilen esansiyel yagin (EY) bazi kultur bitkileri ( Z... more Nepeta transcaucasica Grossh.’dan ekstrakte edilen esansiyel yagin (EY) bazi kultur bitkileri ( Zea mays , Hordeum vulgare, Triticum aestivum) ve zararli otlarin ( Cynodon dactylon, Amaranthus retroflexus, Onopordium acanthium ) tohum cimlenmesi ve fide buyumesi uzerinde herbisidal etkileri arastirilmistir. EY, N. transcaucasica ’dan hidrodistilasyon yontemiyle elde edilmis ve bitkilere ait tohumlar 0, 2, 5, 10 ve 20 µL/L EY konsantrasyonlarinda Petri ortaminda cimlenmeye maruz birakilmistir. Tohumlarin cimlenme yuzdeleri (gunluk) ve fidelerin kok-govde uzunluklari ile kuru agirliklari (6. gun) belirlenmistir. Ilave olarak, cimlenmenin 12., 24. ve 72. saatlerinde tohumlarin endospermlerinde α-amilaz aktivitesi olculmustur. Calismada GC/MS ile analiz edilen N. transcaucasica ’dan elde edilen EY’nin, en fazla nepetalakton (%93.75) icerdigi belirlenmistir. EY, tum konsantrasyonlarinda, zararli ot tohumlarinin cimlenmesini hem geciktirmis hem de onemli oranda inhibe etmistir. Inhibisyon...
TURKISH JOURNAL OF BOTANY, 2019
We derived a protein hydrolysate with rich content, which includes proteinogenic and nonproteinog... more We derived a protein hydrolysate with rich content, which includes proteinogenic and nonproteinogenic amino acids, from chicken feathers by using an alkaline hydrolysis procedure with high yield success. After foliar application of aqueous solutions (0.05%, 0.075%, and 0.1%) of chicken feather protein hydrolysate (CFPH) to the seedlings of wheat (Triticum aestivum L. ' Altındane' and 'Bezostaya'), their effects were evaluated on important physiological and biochemical parameters. Treating plants with CFPH stimulated the expression of RuBisCo and increased root and shoot lengths, fresh and dry weights of the seedlings, and photosynthetic pigment content. In addition, applying CFPH at 0.075% and 0.1% concentrations decreased the content of reactive oxygen species, such as O 2 .and H 2 O 2 , and the levels of lipid peroxidation (as malondialdehyde) and phenolic compounds, while augmenting free proline content. On the contrary, CFPH application ameliorated the antioxidant parameters, including enzymatic and nonenzymatic antioxidants. CFPH stimulated the activities of guaiacol, ascorbate, and glutathione peroxidase while it did not affect those of superoxide dismutase and catalase. Enzyme activity results agreed well with the isoenzyme expression profiles. In conclusion, the application of CFPH improved the parameters under consideration in wheat seedlings, suggesting that CFPH has the potential to be used as a suitable biostimulant in plant cultivation.
Cryobiology, 2018
We have isolated psychrotolerant bacteria from the leaf apoplast of cold-adapted wild plants and ... more We have isolated psychrotolerant bacteria from the leaf apoplast of cold-adapted wild plants and aimed to investigate their effect on the cold resistance of bean (Phaseolus vulgaris L.). Based on the findings of 16S rRNA gene sequence analysis, 20 isolates belonging to 5 bacteria species (Pseudomonas fragi, P. chloropaphis, P. fluorescens, P. proteolytica and Brevibacterium frigoritolerans) were identified in the leaf apoplastic fluid of Draba nemorosa, Galanthus gracilis, Colchicum speciousum, Scilla siberica, Erodium cicutarium, respectively. We have determined that 6 of the 20 isolates have exhibited ACC (1-aminocyclopropane-1-carboxylate) deaminase activity and secreted different extracellular proteins under cold condition (+4 °C) compared to normal growth condition (28 °C). The six isolates were then inoculated independently of each other to the leaves of 10-day-old bean seedlings growing under normal conditions (25/22 °C, 16/8 h photoperiod), and the inoculated and uninoculated (control) seedlings were transferred to cold (9/5°C, 16/8 h photoperiod) for 3 days. The bacterial inoculations have decreased freezing injury, ice nucleating activity and lipid peroxidation content in parallel with the decrease of reactive oxygen species level such as O 2 .and H 2 O 2 in the inoculated seedlings compared to the control. In addition, the inoculations of the isolates have stimulated the activity of apoplastic antioxidant enzymes including superoxide dismutase, catalase, peroxidase, and glutathione reductase. The results show that the inoculations improve the cold resistance of bean seedlings and the psychrotolerant bacterial isolates can be evaluated within the group of plant growth promoting bacteria (PGPB) which can increase tolerance of cold-sensitive crops.
Indian Journal of Plant Physiology, 2007
The present work describes the changes that take place in plant growth substance (PGS) levels, ge... more The present work describes the changes that take place in plant growth substance (PGS) levels, germination and seedling growth during germination of seeds of Medicago sativa cv. Clover, Trifolium repens cv. Milka and Trifolium pratense cv. Bora which had been stored for 37 years. The endogenous abscisic acid (ABA), indole-3-acetic acid (IAA), gibberellic acid (GA 3 ) and zeatin (Z) in the seeds were extracted and finally purified by high performance liquid chromatography (HPLC). Per cent germination, seedling height and the PGS levels in the germinating seeds were determined for 5 days at 23 °C. On day 5, the per cent germination values of aged seeds of M. sativa, T. repens and T. pratense were 37%, 30% and 17%, respectively. The height of seedlings derived from the stored seeds was also significantly low, compared to that of controls. ABA level was high, throughout germination, in the aged seeds studied, but the levels of IAA, GA 3 and Z were low. Based on these results, it can be proposed that the reduced capability of germination and seedling vigour and growth in the aged seeds may be due to high ABA level which remained so throughout germination. This was also associated with decreased levels of GA 3 , IAA and Z.
This study was carried out to evaluate the effect of long-term natural aging on germination capab... more This study was carried out to evaluate the effect of long-term natural aging on germination capability and several biochemical characteristics regarding antioxidative response of both the dry seeds and the during germination of alfalfa (Medicago sativa L.) seeds stored for 42 years. Percent germination ratio of the seeds was monitored for 7 days. The activities of catalase, peoroxidase and superoxide dismutase, lipid peroxidation level, and the contents of phenolic matter and H 2 O 2 were tested in both dry seeds and germinating seeds on 1 st , 3 rd and 7 th days of germination. On the final day of seed germination, the percent germination was 35% in the 42-years old seeds while %96 in the control seeds (non-aged). In addition, the long-term aging caused an important increase in lipid peroxidation and total phenolic content in the aged dry seeds. Long-term aged seeds showed low H 2 O 2 content and the activities of catalase, peroxidase and superoxide dismutase. Decreased germination...
Toxicology and Industrial Health, 2012
The effects of nitric oxide (NO) on chilling tolerance (contents of hydrogen peroxide (H2O2) and ... more The effects of nitric oxide (NO) on chilling tolerance (contents of hydrogen peroxide (H2O2) and superoxide anion (O2–) and lipid peroxidation level (malondialdehyde, MDA)) and the activities of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT)) were investigated in the leaves of wheat ( Triticum aestivum L.) exposed to chilling. NO treatment was carried out through spraying of sodium nitroprusside (SNP), which is a donor of NO. To do this, SNP concentrations of 0.1 and 1 mM were applied on the leaves of 11-day plants and the plants were then exposed to chilling conditions (5/2°C) for 3 days. The chilling stress treatment increased both the activities of antioxidant enzymes and the levels of MDA, H2O2 and O2–. Similarly, NO treatment enhanced SOD, POX and CAT activities under chilling stress, whereas it decreased H2O2 and O2.– contents as well as MDA level. The most effective concentration was determined as 0.1 mM SNP. Exogenous SNP application as ...
Toxicology and Industrial Health, 2012
The aim of this study was to investigate the possible oxidative stress and the antioxidant respon... more The aim of this study was to investigate the possible oxidative stress and the antioxidant response, which were caused on maize by boron (B). For this, 11- and 15-day-old maize seedlings were subjected to 2 or 4 mM B in the form of boric acid (H3BO3) for 2 and/or 6 days. At the end of the treatment period, root length, hydrogen peroxide (H2O2) content, malondialdehyde (MDA) content and the antioxidant enzymes superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT) were measured. The results revealed that root length of plants, activity of antioxidative enzymes such as SOD, POX and CAT and also H2O2 contents and MDA levels were seriously affected by excess B. These results suggested that the oxidative stress occurred due to the toxic effect of B.
Phytochemistry, 1999
Freezing tolerance was determined in cabbage (Brassica oleracea cv Acephala) and winter wheat (Tr... more Freezing tolerance was determined in cabbage (Brassica oleracea cv Acephala) and winter wheat (Triticum aestivum cv DogAE u-88) leaves growing under control and cold conditions. Freezing injury was less in cold-acclimated leaves than in control leaves. In cold-acclimated leaves, the freezing injury increased when apoplastic solution is extracted. In addition, ice nucleation activity was lower with apoplastic proteins extracted from cold-acclimated leaves than from control leaves. These results suggest that the proteins present in the apoplast during cold acclimation are an important component of the mechanism by which cabbage and winter wheat leaves inhibit extracellular ice formation. Winter wheat has greater freezing tolerance than cabbage because winter wheat leaves have lower freezing injury and apoplastic proteins in winter wheat leaves have lower ice nucleation activity.
Journal of the Science of Food and Agriculture, 2011
This study investigated changes in peroxidase (POX) and polyphenol oxidase (PPO) activities throu... more This study investigated changes in peroxidase (POX) and polyphenol oxidase (PPO) activities through adventitious rooting in hardwood cuttings of grapevine rootstocks. Three grapevine rootstocks with different propensity to produce adventitious roots were selected: recalcitrant (Ramsey), non-recalcitrant (Rupestris du Lot) and intermediate (99R) cultivars. The averages of root number at 65 days were 96 in Lot, 76 in 99R and 30 in Ramsey. Both enzyme activities characteristically increased before adventitious rooting, regardless of rooting ability of the rootstocks, and then decreased. POX activity increased in Ramsey cuttings at 22 days, in Lot and 99R cuttings at 14 days after planting, and then decreased gradually until 51 days. The highest POX activity was determined in Ramsey rootstock with the highest rooting ability and the lowest activity was determined in the rootstocks with the lowest rooting ability. PPO activity gradually increased in Ramsey rootstock cuttings from 10 days to 22 days, in Lot and 99R cuttings at 14 days, and then decreased until 51 days. A significant correlation was identified between high POX activity and adventitious rooting capability in rootstocks, but the same result was not determined with PPO activity. A recalcitrant rooting variety cannot increase POX activity sufficiently before rooting. Therefore applications that could increase POX activity in stem cuttings during rooting may facilitate increased rooting in such rootstocks.
Symbiosis, 2005
Résumé/Abstract We investigated effect of putrescine on inducing symbiosis of strains of Rhizobiu... more Résumé/Abstract We investigated effect of putrescine on inducing symbiosis of strains of Rhizobium leguminosarum in commercial inoculants and strains isolated from local soils inoculated to roots of chickpea (Cicer arietinum cv. Aziziye-94) or vetch (Vicia sativa cv. ...
Phytochemistry, 2003
Overwintering plants produce antifreeze proteins (AFPs) having the ability to adsorb onto the sur... more Overwintering plants produce antifreeze proteins (AFPs) having the ability to adsorb onto the surface of ice crystals and modify their growth. Recently, several AFPs have been isolated and characterized and five full-length AFP cDNAs have been cloned and characterized in higher plants. The derived amino acid sequences have shown low homology for identical residues. Theoretical and experimental models for structure of Lolium perenne AFP have been proposed. In addition, it was found that the hormone ethylene is involved in regulating antifreeze activity in response to cold. In this review, it is seen that the physiological and biochemical roles of AFPs may be important to protect the plant tissues from mechanical stress caused by ice formation.
Hacettepe Journal of Biology and Chemistry
B u çalışmada, Datura stramonium L. (boru çiçeği) bitkisinin toprak ve sulak alanlardan 2,4-Diklo... more B u çalışmada, Datura stramonium L. (boru çiçeği) bitkisinin toprak ve sulak alanlardan 2,4-Diklorofenolün (2,4-DKF) fitoremediasyon potansiyeli araştırıldı. Hidroponik sistemde büyütülen fidelerin yetişme ortamı 2,4-DKF'nin farklı konsantrasyonlarına (0.0, 75, 100, 125, 150, 175, 200, 225, 250 ve 275 ppm) ayarlandı. Dört gün sonra, yetişme ortamında 2,4-DKF'nin remediasyon oranı, bitkilerin kök-gövde uzunluğu, kök-gövde kuru ağırlığı, lipid peroksidasyonu (LPO), protein ve fotosentetik pigmentlerin içeriği belirlendi. D. stramonium fideleri çalışılan tüm konsantrasyonlarda 2,4-DKF'nin remediasyonunu %52-78 oranlarında sağladı. Ayrıca 2,4-DKF uygulamaları, fidelerin kök ve gövde uzunlukları ile kuru ağırlıklarını, kontrollerine göre, özellikle 200-275 ppm gibi yüksek dozlarda genelde hafif derecede inhibe ederken, düşük dozlarda etkilemedi. Uygulamalar, genelde (LPO) ve protein içeriğini hafif derecede artırırken, yaprak klorofil içeriğini etkilememiştir. Bulgular, D. stramonium'un 2,4-DKF'nin fitoremediasyonunda kullanılabilme potansiyelinin yüksek olduğunu göstermektedir.
Environmental Science and Pollution Research
Turkish Journal of Botany
The time-dependent effect of salicylic acid (SA) on alleviating cold damage in the Tokak (toleran... more The time-dependent effect of salicylic acid (SA) on alleviating cold damage in the Tokak (tolerant) and Akhisar (sensitive) varieties of barley (Hordeum vulgare) was investigated. Seedlings subjected to 0.0, 0.1, and 1 mM SA for 7 days were transferred to cold (7/5 °C) on days 7, 14, 21, and 28 for 3 days. It was found that 0.1 mM SA had a greater alleviating effect, decreasing the freezing injury in both varieties for at least 10 days after the application. Moreover, 0.1 mM SA had a greater effect on alleviation in Tokak by increasing the ice nucleation activity of apoplastic proteins extracted from the leaves. SA was effective for at least 24 days in decreasing the effect of freezing temperature by regulating apoplastic proteins in the leaves after the application. Furthermore, 0.1 and 1 mM SA had an alleviating effect by decreasing the lipid peroxidation level in both varieties for at least 24 days after the application. It can be seen that SA treatment can play a positive role in alleviating cold damage in barley. Therefore, it is suggested that results from SA treatments may contribute to research related to diminishing cold damage in agricultural applications.
Turkish Journal of Botany, 1997
Abstract: The ability of the polyphenoloxidase (PPO) enzyme in the embryos and food tissues of th... more Abstract: The ability of the polyphenoloxidase (PPO) enzyme in the embryos and food tissues of the seeds of bean (Phaseolus vulgaris L. cv. Magnum) and corn (Zea mays L. cv. Merit) to oxidize the substrates of dopa and catechol was studied in order to record how it is ...
Giriş: Bu çalışmada, bor toksisitesine maruz bırakılan mısır (Zea mays L. cv. Arifiye-2) bitkisin... more Giriş: Bu çalışmada, bor toksisitesine maruz bırakılan mısır (Zea mays L. cv. Arifiye-2) bitkisine çimlenme öncesi tohumlara uygulanan nitrik oksit’in (NO) antioksidan enzimlerden süperoksit dismutaz (SOD), peroksidaz (POX) ve katalaz (CAT) aktiviteleri üzerine etkileri belirlenmiştir. Buna ilave olarak, oksidatif stresin önemli parametrelerinden hidrojen peroksit (H2O2), süperoksit anyonu (O2 .) ve lipid peroksidasyon (MDA) seviyeleri de belirlenerek antioksidan enzimlerle birlikte değerlendirilmiştir.
Gereçler ve Yöntemler: Mısır tohumları çimlendirilmeden önce 100 μM sodyum nitroprussid (NO veric... more Gereçler ve Yöntemler: Mısır tohumları çimlendirilmeden önce 100 μM sodyum nitroprussid (NO vericisi) ile 24 saat boyunca şişirilmeye bırakılmıştır. Bu süre sonunda çimlendirilen ve normal şartlarda (25/22 o C) yetiştirilen bitkilere tohum ekiminin 9. gününde 2 mM borik asit (H3BO3) uygulanmıştır. 2 ve 6 gün boyunca borik asit muamelesine maruz bırakılan bitkilerin yaprakları kesilerek bitki boyu, kuru ağırlık, yaş ağırlık ve iyon geçirgenliğine bakılmıştır.
Australian Journal of Crop Science, 2013
Nitric Oxide (NO) is an important signal molecule modulating the plants responses to abiotic stre... more Nitric Oxide (NO) is an important signal molecule modulating the plants responses to abiotic stresses. In this study the effects of exogenous NO as sodium nitroprusside (SNP) on boron (B)-induced oxidative damage and growth in maize (Zea mays L.) were investigated. The addition of B significantly reduced the growth of plants and increased the values of electrolyte leakage, malondialdehyde (MDA) and hydrogen peroxide (H2O2) contents. SNP (100 uM) that was applied to seeds before germination significantly increased plant height (respectively, 8 and 5%), fresh weight (respectively, 9 and 6%), and dry weight (respectively, 15 and 12%) of both 11 and 15 day old maize. Furthermore, the measured B-induced oxidative stress increased MDA, electrolyte leakage, H2O2 content when compared to a supplementation of NO. SNP application also increased activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and peroxidase (POX). The results suggest that the increased ...
Acta Physiologiae Plantarum, 2021
Nepeta transcaucasica Grossh.’dan ekstrakte edilen esansiyel yagin (EY) bazi kultur bitkileri ( Z... more Nepeta transcaucasica Grossh.’dan ekstrakte edilen esansiyel yagin (EY) bazi kultur bitkileri ( Zea mays , Hordeum vulgare, Triticum aestivum) ve zararli otlarin ( Cynodon dactylon, Amaranthus retroflexus, Onopordium acanthium ) tohum cimlenmesi ve fide buyumesi uzerinde herbisidal etkileri arastirilmistir. EY, N. transcaucasica ’dan hidrodistilasyon yontemiyle elde edilmis ve bitkilere ait tohumlar 0, 2, 5, 10 ve 20 µL/L EY konsantrasyonlarinda Petri ortaminda cimlenmeye maruz birakilmistir. Tohumlarin cimlenme yuzdeleri (gunluk) ve fidelerin kok-govde uzunluklari ile kuru agirliklari (6. gun) belirlenmistir. Ilave olarak, cimlenmenin 12., 24. ve 72. saatlerinde tohumlarin endospermlerinde α-amilaz aktivitesi olculmustur. Calismada GC/MS ile analiz edilen N. transcaucasica ’dan elde edilen EY’nin, en fazla nepetalakton (%93.75) icerdigi belirlenmistir. EY, tum konsantrasyonlarinda, zararli ot tohumlarinin cimlenmesini hem geciktirmis hem de onemli oranda inhibe etmistir. Inhibisyon...
TURKISH JOURNAL OF BOTANY, 2019
We derived a protein hydrolysate with rich content, which includes proteinogenic and nonproteinog... more We derived a protein hydrolysate with rich content, which includes proteinogenic and nonproteinogenic amino acids, from chicken feathers by using an alkaline hydrolysis procedure with high yield success. After foliar application of aqueous solutions (0.05%, 0.075%, and 0.1%) of chicken feather protein hydrolysate (CFPH) to the seedlings of wheat (Triticum aestivum L. ' Altındane' and 'Bezostaya'), their effects were evaluated on important physiological and biochemical parameters. Treating plants with CFPH stimulated the expression of RuBisCo and increased root and shoot lengths, fresh and dry weights of the seedlings, and photosynthetic pigment content. In addition, applying CFPH at 0.075% and 0.1% concentrations decreased the content of reactive oxygen species, such as O 2 .and H 2 O 2 , and the levels of lipid peroxidation (as malondialdehyde) and phenolic compounds, while augmenting free proline content. On the contrary, CFPH application ameliorated the antioxidant parameters, including enzymatic and nonenzymatic antioxidants. CFPH stimulated the activities of guaiacol, ascorbate, and glutathione peroxidase while it did not affect those of superoxide dismutase and catalase. Enzyme activity results agreed well with the isoenzyme expression profiles. In conclusion, the application of CFPH improved the parameters under consideration in wheat seedlings, suggesting that CFPH has the potential to be used as a suitable biostimulant in plant cultivation.
Cryobiology, 2018
We have isolated psychrotolerant bacteria from the leaf apoplast of cold-adapted wild plants and ... more We have isolated psychrotolerant bacteria from the leaf apoplast of cold-adapted wild plants and aimed to investigate their effect on the cold resistance of bean (Phaseolus vulgaris L.). Based on the findings of 16S rRNA gene sequence analysis, 20 isolates belonging to 5 bacteria species (Pseudomonas fragi, P. chloropaphis, P. fluorescens, P. proteolytica and Brevibacterium frigoritolerans) were identified in the leaf apoplastic fluid of Draba nemorosa, Galanthus gracilis, Colchicum speciousum, Scilla siberica, Erodium cicutarium, respectively. We have determined that 6 of the 20 isolates have exhibited ACC (1-aminocyclopropane-1-carboxylate) deaminase activity and secreted different extracellular proteins under cold condition (+4 °C) compared to normal growth condition (28 °C). The six isolates were then inoculated independently of each other to the leaves of 10-day-old bean seedlings growing under normal conditions (25/22 °C, 16/8 h photoperiod), and the inoculated and uninoculated (control) seedlings were transferred to cold (9/5°C, 16/8 h photoperiod) for 3 days. The bacterial inoculations have decreased freezing injury, ice nucleating activity and lipid peroxidation content in parallel with the decrease of reactive oxygen species level such as O 2 .and H 2 O 2 in the inoculated seedlings compared to the control. In addition, the inoculations of the isolates have stimulated the activity of apoplastic antioxidant enzymes including superoxide dismutase, catalase, peroxidase, and glutathione reductase. The results show that the inoculations improve the cold resistance of bean seedlings and the psychrotolerant bacterial isolates can be evaluated within the group of plant growth promoting bacteria (PGPB) which can increase tolerance of cold-sensitive crops.
Indian Journal of Plant Physiology, 2007
The present work describes the changes that take place in plant growth substance (PGS) levels, ge... more The present work describes the changes that take place in plant growth substance (PGS) levels, germination and seedling growth during germination of seeds of Medicago sativa cv. Clover, Trifolium repens cv. Milka and Trifolium pratense cv. Bora which had been stored for 37 years. The endogenous abscisic acid (ABA), indole-3-acetic acid (IAA), gibberellic acid (GA 3 ) and zeatin (Z) in the seeds were extracted and finally purified by high performance liquid chromatography (HPLC). Per cent germination, seedling height and the PGS levels in the germinating seeds were determined for 5 days at 23 °C. On day 5, the per cent germination values of aged seeds of M. sativa, T. repens and T. pratense were 37%, 30% and 17%, respectively. The height of seedlings derived from the stored seeds was also significantly low, compared to that of controls. ABA level was high, throughout germination, in the aged seeds studied, but the levels of IAA, GA 3 and Z were low. Based on these results, it can be proposed that the reduced capability of germination and seedling vigour and growth in the aged seeds may be due to high ABA level which remained so throughout germination. This was also associated with decreased levels of GA 3 , IAA and Z.
This study was carried out to evaluate the effect of long-term natural aging on germination capab... more This study was carried out to evaluate the effect of long-term natural aging on germination capability and several biochemical characteristics regarding antioxidative response of both the dry seeds and the during germination of alfalfa (Medicago sativa L.) seeds stored for 42 years. Percent germination ratio of the seeds was monitored for 7 days. The activities of catalase, peoroxidase and superoxide dismutase, lipid peroxidation level, and the contents of phenolic matter and H 2 O 2 were tested in both dry seeds and germinating seeds on 1 st , 3 rd and 7 th days of germination. On the final day of seed germination, the percent germination was 35% in the 42-years old seeds while %96 in the control seeds (non-aged). In addition, the long-term aging caused an important increase in lipid peroxidation and total phenolic content in the aged dry seeds. Long-term aged seeds showed low H 2 O 2 content and the activities of catalase, peroxidase and superoxide dismutase. Decreased germination...
Toxicology and Industrial Health, 2012
The effects of nitric oxide (NO) on chilling tolerance (contents of hydrogen peroxide (H2O2) and ... more The effects of nitric oxide (NO) on chilling tolerance (contents of hydrogen peroxide (H2O2) and superoxide anion (O2–) and lipid peroxidation level (malondialdehyde, MDA)) and the activities of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT)) were investigated in the leaves of wheat ( Triticum aestivum L.) exposed to chilling. NO treatment was carried out through spraying of sodium nitroprusside (SNP), which is a donor of NO. To do this, SNP concentrations of 0.1 and 1 mM were applied on the leaves of 11-day plants and the plants were then exposed to chilling conditions (5/2°C) for 3 days. The chilling stress treatment increased both the activities of antioxidant enzymes and the levels of MDA, H2O2 and O2–. Similarly, NO treatment enhanced SOD, POX and CAT activities under chilling stress, whereas it decreased H2O2 and O2.– contents as well as MDA level. The most effective concentration was determined as 0.1 mM SNP. Exogenous SNP application as ...
Toxicology and Industrial Health, 2012
The aim of this study was to investigate the possible oxidative stress and the antioxidant respon... more The aim of this study was to investigate the possible oxidative stress and the antioxidant response, which were caused on maize by boron (B). For this, 11- and 15-day-old maize seedlings were subjected to 2 or 4 mM B in the form of boric acid (H3BO3) for 2 and/or 6 days. At the end of the treatment period, root length, hydrogen peroxide (H2O2) content, malondialdehyde (MDA) content and the antioxidant enzymes superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT) were measured. The results revealed that root length of plants, activity of antioxidative enzymes such as SOD, POX and CAT and also H2O2 contents and MDA levels were seriously affected by excess B. These results suggested that the oxidative stress occurred due to the toxic effect of B.
Phytochemistry, 1999
Freezing tolerance was determined in cabbage (Brassica oleracea cv Acephala) and winter wheat (Tr... more Freezing tolerance was determined in cabbage (Brassica oleracea cv Acephala) and winter wheat (Triticum aestivum cv DogAE u-88) leaves growing under control and cold conditions. Freezing injury was less in cold-acclimated leaves than in control leaves. In cold-acclimated leaves, the freezing injury increased when apoplastic solution is extracted. In addition, ice nucleation activity was lower with apoplastic proteins extracted from cold-acclimated leaves than from control leaves. These results suggest that the proteins present in the apoplast during cold acclimation are an important component of the mechanism by which cabbage and winter wheat leaves inhibit extracellular ice formation. Winter wheat has greater freezing tolerance than cabbage because winter wheat leaves have lower freezing injury and apoplastic proteins in winter wheat leaves have lower ice nucleation activity.
Journal of the Science of Food and Agriculture, 2011
This study investigated changes in peroxidase (POX) and polyphenol oxidase (PPO) activities throu... more This study investigated changes in peroxidase (POX) and polyphenol oxidase (PPO) activities through adventitious rooting in hardwood cuttings of grapevine rootstocks. Three grapevine rootstocks with different propensity to produce adventitious roots were selected: recalcitrant (Ramsey), non-recalcitrant (Rupestris du Lot) and intermediate (99R) cultivars. The averages of root number at 65 days were 96 in Lot, 76 in 99R and 30 in Ramsey. Both enzyme activities characteristically increased before adventitious rooting, regardless of rooting ability of the rootstocks, and then decreased. POX activity increased in Ramsey cuttings at 22 days, in Lot and 99R cuttings at 14 days after planting, and then decreased gradually until 51 days. The highest POX activity was determined in Ramsey rootstock with the highest rooting ability and the lowest activity was determined in the rootstocks with the lowest rooting ability. PPO activity gradually increased in Ramsey rootstock cuttings from 10 days to 22 days, in Lot and 99R cuttings at 14 days, and then decreased until 51 days. A significant correlation was identified between high POX activity and adventitious rooting capability in rootstocks, but the same result was not determined with PPO activity. A recalcitrant rooting variety cannot increase POX activity sufficiently before rooting. Therefore applications that could increase POX activity in stem cuttings during rooting may facilitate increased rooting in such rootstocks.
Symbiosis, 2005
Résumé/Abstract We investigated effect of putrescine on inducing symbiosis of strains of Rhizobiu... more Résumé/Abstract We investigated effect of putrescine on inducing symbiosis of strains of Rhizobium leguminosarum in commercial inoculants and strains isolated from local soils inoculated to roots of chickpea (Cicer arietinum cv. Aziziye-94) or vetch (Vicia sativa cv. ...
Phytochemistry, 2003
Overwintering plants produce antifreeze proteins (AFPs) having the ability to adsorb onto the sur... more Overwintering plants produce antifreeze proteins (AFPs) having the ability to adsorb onto the surface of ice crystals and modify their growth. Recently, several AFPs have been isolated and characterized and five full-length AFP cDNAs have been cloned and characterized in higher plants. The derived amino acid sequences have shown low homology for identical residues. Theoretical and experimental models for structure of Lolium perenne AFP have been proposed. In addition, it was found that the hormone ethylene is involved in regulating antifreeze activity in response to cold. In this review, it is seen that the physiological and biochemical roles of AFPs may be important to protect the plant tissues from mechanical stress caused by ice formation.
Hacettepe Journal of Biology and Chemistry
B u çalışmada, Datura stramonium L. (boru çiçeği) bitkisinin toprak ve sulak alanlardan 2,4-Diklo... more B u çalışmada, Datura stramonium L. (boru çiçeği) bitkisinin toprak ve sulak alanlardan 2,4-Diklorofenolün (2,4-DKF) fitoremediasyon potansiyeli araştırıldı. Hidroponik sistemde büyütülen fidelerin yetişme ortamı 2,4-DKF'nin farklı konsantrasyonlarına (0.0, 75, 100, 125, 150, 175, 200, 225, 250 ve 275 ppm) ayarlandı. Dört gün sonra, yetişme ortamında 2,4-DKF'nin remediasyon oranı, bitkilerin kök-gövde uzunluğu, kök-gövde kuru ağırlığı, lipid peroksidasyonu (LPO), protein ve fotosentetik pigmentlerin içeriği belirlendi. D. stramonium fideleri çalışılan tüm konsantrasyonlarda 2,4-DKF'nin remediasyonunu %52-78 oranlarında sağladı. Ayrıca 2,4-DKF uygulamaları, fidelerin kök ve gövde uzunlukları ile kuru ağırlıklarını, kontrollerine göre, özellikle 200-275 ppm gibi yüksek dozlarda genelde hafif derecede inhibe ederken, düşük dozlarda etkilemedi. Uygulamalar, genelde (LPO) ve protein içeriğini hafif derecede artırırken, yaprak klorofil içeriğini etkilememiştir. Bulgular, D. stramonium'un 2,4-DKF'nin fitoremediasyonunda kullanılabilme potansiyelinin yüksek olduğunu göstermektedir.
Environmental Science and Pollution Research
Turkish Journal of Botany
The time-dependent effect of salicylic acid (SA) on alleviating cold damage in the Tokak (toleran... more The time-dependent effect of salicylic acid (SA) on alleviating cold damage in the Tokak (tolerant) and Akhisar (sensitive) varieties of barley (Hordeum vulgare) was investigated. Seedlings subjected to 0.0, 0.1, and 1 mM SA for 7 days were transferred to cold (7/5 °C) on days 7, 14, 21, and 28 for 3 days. It was found that 0.1 mM SA had a greater alleviating effect, decreasing the freezing injury in both varieties for at least 10 days after the application. Moreover, 0.1 mM SA had a greater effect on alleviation in Tokak by increasing the ice nucleation activity of apoplastic proteins extracted from the leaves. SA was effective for at least 24 days in decreasing the effect of freezing temperature by regulating apoplastic proteins in the leaves after the application. Furthermore, 0.1 and 1 mM SA had an alleviating effect by decreasing the lipid peroxidation level in both varieties for at least 24 days after the application. It can be seen that SA treatment can play a positive role in alleviating cold damage in barley. Therefore, it is suggested that results from SA treatments may contribute to research related to diminishing cold damage in agricultural applications.
Turkish Journal of Botany, 1997
Abstract: The ability of the polyphenoloxidase (PPO) enzyme in the embryos and food tissues of th... more Abstract: The ability of the polyphenoloxidase (PPO) enzyme in the embryos and food tissues of the seeds of bean (Phaseolus vulgaris L. cv. Magnum) and corn (Zea mays L. cv. Merit) to oxidize the substrates of dopa and catechol was studied in order to record how it is ...
Giriş: Bu çalışmada, bor toksisitesine maruz bırakılan mısır (Zea mays L. cv. Arifiye-2) bitkisin... more Giriş: Bu çalışmada, bor toksisitesine maruz bırakılan mısır (Zea mays L. cv. Arifiye-2) bitkisine çimlenme öncesi tohumlara uygulanan nitrik oksit’in (NO) antioksidan enzimlerden süperoksit dismutaz (SOD), peroksidaz (POX) ve katalaz (CAT) aktiviteleri üzerine etkileri belirlenmiştir. Buna ilave olarak, oksidatif stresin önemli parametrelerinden hidrojen peroksit (H2O2), süperoksit anyonu (O2 .) ve lipid peroksidasyon (MDA) seviyeleri de belirlenerek antioksidan enzimlerle birlikte değerlendirilmiştir.
Gereçler ve Yöntemler: Mısır tohumları çimlendirilmeden önce 100 μM sodyum nitroprussid (NO veric... more Gereçler ve Yöntemler: Mısır tohumları çimlendirilmeden önce 100 μM sodyum nitroprussid (NO vericisi) ile 24 saat boyunca şişirilmeye bırakılmıştır. Bu süre sonunda çimlendirilen ve normal şartlarda (25/22 o C) yetiştirilen bitkilere tohum ekiminin 9. gününde 2 mM borik asit (H3BO3) uygulanmıştır. 2 ve 6 gün boyunca borik asit muamelesine maruz bırakılan bitkilerin yaprakları kesilerek bitki boyu, kuru ağırlık, yaş ağırlık ve iyon geçirgenliğine bakılmıştır.
Australian Journal of Crop Science, 2013
Nitric Oxide (NO) is an important signal molecule modulating the plants responses to abiotic stre... more Nitric Oxide (NO) is an important signal molecule modulating the plants responses to abiotic stresses. In this study the effects of exogenous NO as sodium nitroprusside (SNP) on boron (B)-induced oxidative damage and growth in maize (Zea mays L.) were investigated. The addition of B significantly reduced the growth of plants and increased the values of electrolyte leakage, malondialdehyde (MDA) and hydrogen peroxide (H2O2) contents. SNP (100 uM) that was applied to seeds before germination significantly increased plant height (respectively, 8 and 5%), fresh weight (respectively, 9 and 6%), and dry weight (respectively, 15 and 12%) of both 11 and 15 day old maize. Furthermore, the measured B-induced oxidative stress increased MDA, electrolyte leakage, H2O2 content when compared to a supplementation of NO. SNP application also increased activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and peroxidase (POX). The results suggest that the increased ...
Acta Physiologiae Plantarum, 2021