İlhami Çelik - Academia.edu (original) (raw)

Papers by İlhami Çelik

Veterinarski Arhiv, 2018

CELIK, I., M. ŞEKER, A. SALBACAK: Histological and histomorphometric studies on the cerebellar co... more CELIK, I., M. ŞEKER, A. SALBACAK: Histological and histomorphometric studies on the cerebellar cortex and silver stained nucleolus organizer regions of Purkinje neurons in chronic morphine-treated rats. Vet. arhiv 88, 75-88, 2018. ABSTRACT the effects of chronic morphine administration on the rat cerebellum and silver stained nucleolus organizer regions in Purkinje cells were investigated by means of histological, histochemical and histometrical techniques. thirty-two young (30-32 days of age) Wistar rats (equal numbers of both genders) were randomly divided into 2 groups, as control and morphine-treated, each having equal numbers of both genders, a total of 16 animals. the control animals were injected subcutaneously with 1mL/kg physiological saline, and the morphine-treated rats received 5 mg/kg morphine hydrochloride subcutaneously at daily intervals for 30 days. the thickness of the molecular and granular layers of the cerebellum, the diameters of the nuclei and nucleoli of cerebellar Purkinje neurones, and the number and size of the silver staining nucleolus organizer regions of the Purkinje cell nuclei were determined histomorphometrically. the morphine administration caused slight histological changes in the cerebellum. The molecular layer thickness of the cerebellum was significantly (P<0.05) reduced, and the decrease was mainly in the male animals. In contrast, the layer thickened insignificantly in the morphinetreated females. The granular layer thickened slightly but insignificantly in both genders of the morphinetreated group. The Purkinje cell count significantly decreased with morphine treatment. Nucleus size did not change with morphine treatment. however, morphine-treated animals had smaller nucleoli. It was concluded that morphine treatment caused significant histomorphological changes in the cerebellar cortex in a sexually dimorphic manner.

DergiPark (Istanbul University), Jun 1, 2008

Neuro endocrinology letters, 2010

We aimed to investigate the effects of weak extremely low frequency electromagnetic fields (ELF-E... more We aimed to investigate the effects of weak extremely low frequency electromagnetic fields (ELF-EMFs) on the nucleus size, the silver staining nucleolar organizer regions (AgNORs), the frequency of micro nucleated peripheral blood lymphocytes (MPBLs) and the micro nucleated polychromatic erythrocytes (MPCEs). One hundred and twenty Swiss albino mice were equally divided into 6 groups. The study groups were exposed to 1, 2, 3, 4 and 5 microT 50 Hz-EMFs for 40 days. Micronucleus number (MN) per PBL was determined.. ELF-EMF exposure caused a nonlinear decline of nucleus area. A sharp drop occurred in AgNOR area of 1 microT group, and following it gained an insignificantly higher level than that of the control group. The field did not change mean AgNOR numbers per nucleus of the groups. Relative AgNOR area had the highest level in 1 microT-exposure group, and the level was quite similar to that of the 5 microT-exposure group. The remaining groups had significantly lower values quite sim...

International Journal of Scientific and Technological Research, 2015

Revue De Medecine Veterinaire, 2003

Enzyme cytochemical and histochemical tests have practical value for the study of immature, blast... more Enzyme cytochemical and histochemical tests have practical value for the study of immature, blast cells and lymphocytes since Romanowsky-stained films might be insufficient to identify differentiation and maturation features of the cells [2, 7]. Peripheral blood lymphocyte (PBL) counts and their enzymatic profile may change in the human [7] and bovine leukemia [23], Marek's disease in the chicken [16, 21] and lymphotrophic viral diseases, such as canine distemper [1, 46] and caprine encephalo-arthritis [22].

Eurasian Journal of Veterinary Sciences, 2011

Gerec ve Yontem: Onbes adet ergin ve saglikli cincilyadan alinan deri ornekleri tamponlu formol s... more Gerec ve Yontem: Onbes adet ergin ve saglikli cincilyadan alinan deri ornekleri tamponlu formol salin solusyonunda tespit edildi ve rutin histolojik yontemlerle parafinde bloklandi. Bloklardan alinan kesitler Crossmon’un uclu boyamasi, Gordon ve Sweet’in retikuler iplik boyamasi, Verhoef’in elastik iplik boyamasi ve AgNOR boyama yontemleriyle boyandi. Derinin toplam kalinligi ile epidermis ve dermis kalinliklari, birim alandaki (1 mm2) ortalama kil folikul sayilari ve germinal matriks pili hucrelerinde AgNOR sayisi ve alani belirlendi.

Tavuklarin lenfoproliferatif bir hastaligi olan Marek hastaligi (MD), lenfoid lokozis gibi diger ... more Tavuklarin lenfoproliferatif bir hastaligi olan Marek hastaligi (MD), lenfoid lokozis gibi diger lenfoprolifertatif hastaliklar ile benzer klinik belirtiler gosterirler. Hastaligin ayirici tanisi icin yas, sinirsel belirtiler, histopatolojik ve sitolojik bulgular onemli kriterler olmakla birlikte yetersizdir. T lenfositlerin MD’de baskin hucre oldugu bildirilmektedir. Alfa Naftil Asetatat Esteraz (ANAE) tespiti ise tavuklarda T lenfosit tanimlamasinda kullanilan onemli bir yontemdir. Bu calismada, hastalik supheli tavuklardan alinan kan ve doku orneklerinde ANAE demonstrasyonu yapildi ve elde edilen sonuclar makroskobik ve mikroskobik bulgularla karsilastirildi. Marek hastaliginin teshisinde lenfosit enzim histokimya sonuclari ile makroskobik ve histopatolojik sonuclar karsilastirilarak, Marek hastaligi teshisinde enzim histokimyasal bulgular degerlendirildi. ANAE Marek hastalikli tavuklarin hem perifer kanlarinda hem de tumorlu dokularinda onemli derecede yuksek olarak tesbit edild...

Kafkas Universitesi Veteriner Fakultesi Dergisi, 2014

Bu çalışma, farelerde gebeliğin T-lenfosit, null lenfosit ve asit fosfataz (ACP-az) pozitif lenfo... more Bu çalışma, farelerde gebeliğin T-lenfosit, null lenfosit ve asit fosfataz (ACP-az) pozitif lenfositlerin perifer kandaki oranı ve endometriyumun desidua bazalis bölgesindeki dağılımı üzerindeki etkilerinin belirlenmesi amacıyla yapıldı. Bu amaçla, 12-14 haftalık fareler, gebe olmayan-kontrol grubu ile gebeliğin birinci, ikinci ve üçüncü haftalarının ortasına karşılık gelecek şekilde erken, orta ve geç gebelik dönemi (sırasıyla gebeliğin 3., 10. ve 17. günleri) olmak üzere 4 gruba ayrıldılar (n = 6). En düşük T-lenfosit oranı perifer kanda (%43.83) ve desidua bazalis dokusunda (10.83 adet/0.1 mm 2) sırasıyla gebeliğin erken ve orta dönemlerinde tespit edildi. Perifer kan ACP-az pozitif lenfosit oranlarında gebeliğin ikinci haftasında istatistiksel olarak önemli bir yükselme gözlenirken (%44.33); desidua bazalis dokusunda en düşük ACP-az pozitif lenfosit sayısı (5.50 adet/0.1 mm 2) gebeliğin erken döneminde gözlendi. Hem perifer kanda (%11.50) ve hem de desidua bazalis dokusunda (7.83 adet/0.1 mm 2) en yüksek null hücre oranı erken gebelik döneminde tespit edildi. En düşük perifer kan lenfosit oranı (%56.00) erken dönemde gözlendi. Dönemler arasında bazı farklar olsa da gebeliğin lenfosit, T-lenfosit, null lenfosit ve ACP-az pozitif lenfositlerinin sayı ve dağılımlarını etkilediği sonucuna varıldı.

Kafkas Universitesi Veteriner Fakultesi Dergisi, 2012

In this light microscopic study, morphometric parameters of the circumvallate papillae and the nu... more In this light microscopic study, morphometric parameters of the circumvallate papillae and the number of their taste buds in tongues of young (6-9 month-old) and aged (7 year-old) Akkaraman sheep were compared in order to determine general morphology and whether there were age-related differences in general morphology and numbers of the taste buds between young and aged animals. The tongues were obtained from a local slaughterhouse. Tissue samples containing dorsal lingual papillae of 32 tongues (16 young and 16 aged) from both sexes (equal numbers of the males and females) were used for histological investigation. The mean number and diameter of the circumvallate papilla, mean number and diameter of the taste buds, and the taste bud number per circumvallate papilla were determined under the light microscope. There were no significant differences between the young and aged animals in the mean number and diameter of circumvallate papillae, the taste bud number and the diameter, taste bud number per papilla. Nevertheless, the aged animals had larger circumvallate papillae. These results showed that number of the taste buds and circumvallate papilla did not change with aging in Akkaraman sheep. Because that general morphology of the circumvallate papillae and their taste bud numbers did not change even in older animals, any age-related differences in the sense of taste can not be attributed to gross degenerative changes in taste buds of circumvallate papillae.

Kafkas Universitesi Veteriner Fakultesi Dergisi, 2009

In this study detrimental effects of aflatoxin (AF) on the peripheral blood formula and the alpha... more In this study detrimental effects of aflatoxin (AF) on the peripheral blood formula and the alpha naphthyl acetate esterase (ANAE)-positivity profile of peripheral blood lymphocytes (PBL) on the mice were determined. A total of 40 white mice were randomly divided in to 5 groups each having 8 animals. The first group served as a control and received standard diet. The remaining 4 groups were fed diets containing 200, 400, 800 and 1600 ppb AF, respectively. The results of this study have revealed that AF given by the food caused significant declines both in the proportion of PBL and in the percentages of ANAEpositive peripheral blood lymphocytes (T-lymphocytes) in a dose dependent manner. These results may be concerned as a haematological evidence for immuno-suppressive effect of AF on cell-mediated immunity.

fenbildergi.aku.edu.tr

ÖZET Bu çalışmada, iyi bir organik çözücü olan asetonun farklı konsantrasyonlarının embriyotoksis... more ÖZET Bu çalışmada, iyi bir organik çözücü olan asetonun farklı konsantrasyonlarının embriyotoksisitesi döllü tavuk yumurtaları kullanılarak araştırıldı. Bu amaçla, dört farklı konsantrasyondaki (% 30,% 50,% 70 ve% 100 v/v) aseton solüsyonu (20 µl/yumurta) ...

Kaybedilen bir uzvun islevini yerine getirmek, zarar gormus biyolojik bir yapiyi destelemek veya ... more Kaybedilen bir uzvun islevini yerine getirmek, zarar gormus biyolojik bir yapiyi destelemek veya var olan biyolojik bir yapiyi gelistirmek icin gunumuzde siklikla kullanilan implant malzemelerin gerek mekanik gerekse tribolojik ozelliklerinin insan vucuduyla uyumlu olmasi gerekmektedir. Biyouyumluluklari yuksek olan implant malzemeler, cogunlugu sividan mutesekkil ve korozif bir ortam olan insan vucuduyla etkilesime girdiginde iyon salinimi egilimi gostermektedir. Bunun sonucunda implant cevresinde arzu edilmeyen hasar mekanizmalari (iltihaplanma, implant gevsemesi vs.) olusabilmektedir. Bu calismada, konuyla ilgili literaturde mevcut olan arastirmalar ozetlenmis ve bu soruna cozum olabilecek oneriler paylasilmistir.

Kafkas Universitesi Veteriner Fakultesi Dergisi, 2013

Bu çalışma, Merinos ırkı koyunlarda gebeliğin perifer kan lenfositlerinin alfa naftil asetat este... more Bu çalışma, Merinos ırkı koyunlarda gebeliğin perifer kan lenfositlerinin alfa naftil asetat esteraz (ANAE) ve asit fosfataz (ACP-az) aktiviteleri ile perifer kan lenfosit oranları üzerindeki etkilerinin belirlenmesi amacıyla yapıldı. Hayvanlardan her grupta 20'şer adet olacak şekilde gebe olmayan-kontrol, bir aylık, iki aylık, üç aylık, dört aylık ve beş aylık gebeler olmak üzere toplam 6 dönemde perifer kanlar alındı. Histolojik incelemeler sonucunda en düşük ANAE pozitif-lenfosit oranı (%63.5) bir aylık gebe koyunlarda tespit edilirken aynı dönemde null lenfosit sayısı da en yüksek seviyede (%12.75) belirlendi. Gebeliğin son dönemindeki koyunların perifer kan lenfosit (%42.9) ve ACP-az pozitif lenfosit (%43.35) oranlarında da istatistiksel olarak önemli düşüşler gözlendi. Tüm gebelik dönemlerinde perifer kan lenfosit oranlarında hormonal değişimlerin neden olduğu düşünülen dalgalanmalar olsa da en belirgin değişimler gebeliğin ilk ve son dönemlerinde gözlendi. Bu çalışmadan elde edilen bulguların maternal toleransın olası mekanizmalarının anlaşılmasına katkı sağlayabileceği sonucuna varıldı.

Nifedipine is a calcium channel blocker that primarily blocks L-type calcium channels. The aim of... more Nifedipine is a calcium channel blocker that primarily blocks L-type calcium channels. The aim of this study was to evaluate effects onhistology of small intestine of high dose nifedipine, which was administered to the rats for 30 days and also effects of the cessation of theadministration after 40 days. The rats were divided into control (n=16) and nifedipine (n=16) groups at the beginning of the study. Standardrat feed containing 125 mg\kg powdered nifedipine was given to the nifedipine group everyday for one week. During the following threeweeks, 250 mg\kg nifedipine was given to the rats with the same method. On the 30th and 70th days of the study, histologic sections wereobtained from the tissue samples of small intestine. The histological changes occurring in the small intestine were evaluated andhistomorphometric measurements were performed under the light microscope. Goblet cells were also evaluated in the sections stained withperiodic-acid Schiff (PAS) method. A statistical...

American Journal of Veterinary Research, 2002

Objective—To evaluate the efficacy of topical administration of a 2% solution of cyclosporine (Cs... more Objective—To evaluate the efficacy of topical administration of a 2% solution of cyclosporine (CsA) for treatment of dogs with keratoconjunctivitis sicca (KCS) and to correlate results with histopathologic characteristics and local cellular immunity of the gland of the third eyelid. Animals—24 dogs with bilateral KCS. Procedure—Lacrimal secretion was measured, using Schirmer tear test (STT) strips. Leukocyte and T-lymphocyte subsets were determined in blood samples. Histopathologic changes as well as CD4+, CD8+, and alpha-naphthyl-acetate esterase-positive (ANAE+) lymphocytes were evaluated. Results—Clinical signs resolved at the end of 1 month in conjunction with significantly increased STT values, compared with baseline values. Fifteen and 30 days after discontinuation of CsA treatment, a decrease was observed in STT values in both eyes; however, only values for the right eye were significantly different. There was a significant decrease in the number of lymphocytes and ANAE+ lymp...

Bratislava Medical Journal, 2012

Objective: Acrylamide (ACR) is a chemical used in many industries around the world and more recen... more Objective: Acrylamide (ACR) is a chemical used in many industries around the world and more recently was found to be formed naturally in foods cooked at high temperatures. ACR was shown to be a neurotoxicant, reproductive toxicant, and carcinogen in animal species. The aim of the present study is to evaluate the infl uence of ACR treatment on urinary bladder responses to carbachol (10-9-3x10-4 M) and potassium chloride (KCl; 5-100 mM), each of them causes receptor-dependent and receptor-independent contractions, respectively. We also examined the role of gender in these responses. Material and methods: Rats of both genders were divided into three groups as follows: (1) Control animals (2) ACR-I; ACR-treated (2 mg/kg-d for 90 days) (3) ACR-II; ACR-treated (5 mg/kg-d for 90 days). Results: In rats treated with ACR, the EC 50 values of carbachol and KCl, but not the maximal response, to both agents were signifi cantly higher than in control group. Histopathological parameters such as edema, congestion, infl ammatory cells, microvascular proliferation, fi brosis, eosinophils, mast cells and epithelial damage were all higher in the ACR-treated group than in the controls. Conclusions: These results demonstrate for the fi rst time that ACR-treatment can induce urinary bladder injury (Tab. 4, Fig. 4, Ref. 30).

$Research paper thumbnail of Effects of zinc and aprotinin on the healing of ulnar diaphyseal fractures in rabbits$

Acta Veterinaria Brno, 2018

The effects of zinc and aprotinin on fracture healing in experimentally induced fractures were in... more The effects of zinc and aprotinin on fracture healing in experimentally induced fractures were investigated by means of histometric analyses and alkaline phosphatase histochemistry. Healthy 54 adult New Zealand White female rabbits were separated into three groups as control, zinc, and aprotinin treatment. The control animals did not receive any medicament; zinc sulphate was given orally to the rabbits in the Zn group for 15 days. Aprotinin was postoperatively infiltrated into the fracture area at the 3rd and 24th h following operation. Immobilization of fracture ends of all groups was similar throughout the experiment. The zinc administered group displayed the highest alkaline phosphatase positive cell level through the experiment. By day 30 after the operation, fibrocartilage and osseous tissues reached the highest levels in the zinc treated group. Based on the observation of augmented osseous tissue formation and increased alkaline phosphatase positive osteoblastic cell activity ...

Cryopreservation [Working Title], 2019

Low temperature has been utilized to keep living cells and tissues dormant but potentially alive ... more Low temperature has been utilized to keep living cells and tissues dormant but potentially alive for cryopreservation and biobanking with great impacts on scientific and biomedical applications. However, there is a critical contradiction between the purpose of the cryopreservation and experimental findings: the cryopreserved cells and tissues can be fatally damaged by the cryopreservation process itself. Contrary to popular belief, the challenge to the life of living cells and tissues during the cryopreservation is not their ability to endure storage at cryogenic temperatures (below −190°C); rather it is the lethality associated with mass and energy transport within an intermediate zone of low temperature (−15 to −130°C) that a cell must traverse twice, once during cooling and once during warming. This chapter will focus on (1) the mechanisms of cryoinjury and cryopretection of human sperm in cryopreservation, and (2) cryopreservation techniques and methods developed based on the understanding of the above mechanisms.

Eurasian Journal of Veterinary Sciences, 2020

Öz Amaç: Akrozomal enzimlerin seminal plazmaya sızıp sızmadığının belirlenmesi, suni tohumlama iç... more Öz Amaç: Akrozomal enzimlerin seminal plazmaya sızıp sızmadığının belirlenmesi, suni tohumlama için sperma işlenmesi sırasında oluşan akrozom hasarının öncü bir göstergesi olabilir. Alfa-naftil asetat esteraz enzimi bu amaçla kullanılabilecek olan akrozomal enzimlerden biridir. Bu çalışmada, taze ve dondurup-çözdürülen boğa spermatozoonlarındaki alfa-naftil asetat esteraz enziminin pozitivite oranları ve lokalizasyonunun ışık mikroskobuyla belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmada materyal olarak 7 boğadan alınan sperma örneği kullanıldı. Bu amaçla, taze ve dondurulup-çözdürülen sperma örneklerinden sürme frotiler hazırlandı. Bu frotilerde alfa-naftil asetat esteraz enzimi sitokimyasal metotla demonstre edildi. Hazırlanan preparatlar ışık mikroskobuyla incelenerek alfa-naftil-asetat esteraz pozitivite oranları, enzimin lokalizasyon ve dağılım tarzı belirlendi. Bulgular: Alfa-naftil asetat esteraz enzimi pozitivitesi, spermatozoonun akrozomunda lokalize olan kırmızımsı-kahve renkli reaksiyon ürünü halinde gözlendi. Bazı hücrelerde enzimatik reaksiyon akrozom zarında dar bir band halinde gözlendi. Taze sperma örneklerinin alfa-naftil asetat esteraz pozitivitesinde belirgin bireysel farklılıklar gözlendi. Enzimatik reaksiyon, hücrelerin tamamında aynı şiddette değildi. Bazı hücrelerdeki reaksiyon nispeten daha zayıftı. Düşük spermatozoon vitalitesi ve motilitesi tesbit edilen taze sperma örneklerinin alfa-naftil asetat esteraz enzim pozitivitesi de düşüktü. Dondurup-çözme işlemi, örneklerin alfa-naftil asetat esteraz pozitivitesinde önemli düşüşe yol açtı. Öneri: Akrozomal alfa-naftil asetat esteraz enzimi, boğa spermasının fertilite potansiyelinin ve dondurma işlemine uygunluğunun değerlendirilmesinde ve önemli bir parametre olarak dikkate alınabileceği sonucuna varılmıştır.