ŞENGÜL YÜKSEL - Academia.edu (original) (raw)

Papers by ŞENGÜL YÜKSEL

Drug and chemical toxicology, May 10, 2024

PubMed, Feb 1, 2024

Objective: Aripiprazole, risperidone, atomoxetine, and methylphenidate are drugs commonly prescri... more Objective: Aripiprazole, risperidone, atomoxetine, and methylphenidate are drugs commonly prescribed for many psychiatric conditions and can be used alone or in combination in children and adolescents. This study aimed to investigate comparatively the possible genotoxic effects or genoprotective potentials of these drugs on human lymphocytes and HepG2 cells. Materials and methods: Cytotoxicity analysis was performed with the cell viability test on human lymphocytes and HepG2 cells, and half-maximal inhibitory concentration (IC50) values of the drugs were determined, and three different doses (¼ IC50, ½ IC50, and IC50) were applied for genetic analysis. For the determined doses, cells with and without DNA damage were examined by comet analysis. Results: In lymphocytes, aripiprazole and risperidone increased DNA damage at moderate and maximum doses, whereas atomoxetine increased DNA damage only at the maximum dose. In HepG2 cells, risperidone reduced DNA damage at all doses, while atomoxetine increased DNA damage at all doses. On the other hand, in the DNA-damaged cells induced by hydrogen peroxide (H2O2), DNA damage decreased at all concentrations of all drugs in both lymphocytes and HepG2 cells. Conclusions: As a result, the genotoxicity of the drugs was found to be dose-dependent, and all drugs showed a genoprotective effect on DNA-damaged cells.

Annals of medical research, 2001

PubMed, 2016

We here present a rare case of a Turner syndrome with mosaic trisomy 15 identified on chorionic v... more We here present a rare case of a Turner syndrome with mosaic trisomy 15 identified on chorionic villous sampling (CVS). Although there are several reports in the literature indicating confined placental mosaicism (CPM), counseling parents of a fetus with trisomy 15 mosaicism at CVS remains difficult because of the phenotypic variability. To illuminate that condition an amniocentesis or cord blood study should be offered in conjunction with genetic counseling.

Bratislavské lekárske listy, 2017

OBJECTIVE: Protective effect of thymoquinone (TQ) against the cytotoxic and genotoxic effects of ... more OBJECTIVE: Protective effect of thymoquinone (TQ) against the cytotoxic and genotoxic effects of cyclophosphamide (CP) was assessed in human peripheral blood lymphocyte culture. METHODS: Mitotic indices were determined as endpoints of cytotoxicity, while sister chromatid exchanges (SCE) served as endpoints of genotoxicity. Firstly, the genotoxic effect of 0.16 μg/ml of CP was tested and CP was detected as genotoxic. In the second set, CP group was treated with 20 μM and 40 μM TQ. RESULTS: TQ reduced the SCE frequencies, suggesting its protective action on human lymphocytes in vitro against the CP induced genotoxic damage. CONCLUSIONS: Our results suggest that TQ produces a protective mechanism against CP-induced genetic damage, and suggest a role of DNA strand breaks in the genotoxicity (Tab. 1, Fig. 1, Ref. 19).

Eurasian journal of medicine and oncology, 2017

T he frequency of human chromosome anomalies (numerical or structural) is reported to be 7.5% in ... more T he frequency of human chromosome anomalies (numerical or structural) is reported to be 7.5% in the general population. [1] In humans, chromosomal anomaly, such as pericentric inversion, is observed in all chromosomes, except chromosome 20. In many cases, phenotypic anomaly is not observed as a result of inversions in heterochromatin areas, such as 1qh, 9qh, 16qh, and this condition is called polymorphism. [2] Pericentric inversion of chromosome 9 is considered as one of the most prevalent variations of the human karyotype. [3] Inversion of chromosome 9 is frequently found in normal individuals, and its frequency in the general population is expected to be approximately 1%-3%. [1] Since the clinical significance of inv (9) was defined in 1972, it has become a matter of debate. For instance, the last version of International System for Cytogenetic Nomenclature (ISCN) mentioned inv (9) (p12q13) as a chromosome polymorphism without clinical significance. However, several authors supported possible correlation between inv (9) and certain clinical diagnoses similar to congenital anomalies, such as schizophrenia, increased Objectives: One of the most common, structurally balanced chromosome rearrangements is the pericentric inversion of chromosome (inv[9]). It is considered to be a variant of the normal karyotype, and has been found in both normal populations and patients with various abnormal phenotypes. The aim of this study was to determine the frequency of chromosome 9 rearrangement in the Malatya Province and check whether it is correlated with certain diseases. Methods: In this study, we investigated the karyotype analysis of 4168 patients admitted in the

International Journal of Biological Sciences, 2016

Cyclophosphamide (CYP) induced hemorrhagic cystitis is a dose-limiting side effect involving incr... more Cyclophosphamide (CYP) induced hemorrhagic cystitis is a dose-limiting side effect involving increased oxidative stress, inflammatory cytokines and suppressed activity of nuclear factor related erythroid 2-related factor (Nrf2). Thymoquinone (TQ), an active constituent of Nigella sativa seeds, is reported to increase the expression of Nrf2, exert antioxidant action, and anti-inflammatory effects in the experimental animals. The present study was designed to explore the effects of TQ on CYP-induced hemorrhagic cystitis in Balb/c mice. Cystitis was induced by a single intraperitoneal injection of CYP (200 mg/kg). TQ was administered intraperitoneally at 5, 10 and 20 mg/kg doses twice a day, for three days before and three days after the CYP administration. The efficacy of TQ was determined in terms of the protection against the CYP-induced histological perturbations in the bladder tissue, reduction in the oxidative stress, and inhibition of the DNA fragmentation. Immunohistochemistry was performed to examine the expression of Nrf2. TQ protected against CYP-induced oxidative stress was evident from significant reduction in the lipid peroxidation, restoration of the levels of reduced glutathione, catalase and superoxide dismutase activities. TQ treatment significantly reduced the DNA damage evident as reduced DNA fragmentation. A significant decrease in the cellular infiltration, edema, epithelial denudation and hemorrhage were observed in the histological observations. There was restoration and rise in the Nrf2 expression in the bladder tissues of mice treated with TQ. These results confirm that, TQ ameliorates the CYP-induced hemorrhagic cystitis in mice through reduction in the oxidative stress, inhibition of the DNA damage and through increased expression of Nrf2 in the bladder tissues.

Pathophysiology, 2002

Acute stress known to stimulate sympathetic activity as well as the hypothalamo-pituitary–adrenal... more Acute stress known to stimulate sympathetic activity as well as the hypothalamo-pituitary–adrenal (HPA) axis, produces a significant increase in adrenomedullin (ADM) levels in the pituitary gland, plasma and adrenal glands, all of which are key components of HPA axis, suggesting a regulatory or protective role for ADM in countering HPA activation following a variety of physiological and psychological stressors. This

Russian Journal of Genetics, 2012

We designed in vitro study to determine possible genotoxic effects oftacrolimus (FK-506), which i... more We designed in vitro study to determine possible genotoxic effects oftacrolimus (FK-506), which is used as a potent immunosuppressive drug, by using sister chromatid exchange (SCEs), chromosome aberration (CAs), micronuclei tests (MN) and cell growth kinetics such as mitotic index (MI) and replication index (RI) in human lymphocytes. The cells were treated with 5, 25, 50, and 100 ng/mL concentrations of tacrolimus, for 24 h and 48 h treatment periods. Tacrolimus induced CA and MN frequency at all concentrations for 24 and 48 h In additon, it induced the SCE at the highest concantration for 24 h and at 25 and 100 ng/mL for 48 h. Tacrolimus decreased MI at all concentrations (except 5 ng/mL) for all treatment periods. It also inhibited the RI at 50 and 100 ng/mL concentrations for 24 h and at all concentrations for 48 h. Treatments given with tacrolimus result in the enhance of the different endpoints ofgenotoxicity, suggesting its mutagenic action on lymphocytes in vitro.

Although possible associations between supernumerary nipples and other congenital anomalies has b... more Although possible associations between supernumerary nipples and other congenital anomalies has been emphasized, diastometamyelia and polymastia which has not been has not been reported as an association of these two anomalies before. In this report, we describe a 11 years-old girl with diastometamyelia and polymastia and we investigated genetic etiology of her complex phenotype. Çoklu meme oluşumu ile diğer konjenital anomaliler arasında olası ilişkiler gösterilmiş olmasına karşın diastometamyeli ve polimasti anomalilerinin ilişkisi daha önce bildirilmemiştir. Bu vakada, diastometamyeli ve polymastili on bir yaşındaki bir kız hasta kompleks fenotipinin genetik etiyolojisi ile birlikte araştırılarak sunuldu. Anahtar Kelimeler: Polimasti; Diastometamyeli; Genetik. Ectopic breast tissue occurs in 0.4-6% of the general population. Usually these tissues develope along the embryonic milk line. Although polythelia is congenital in origin and is identifiable at childhood, polymastia may no...

Turkish Journal of Medical Sciences

The aim of this study was to determine the prevalence of consanguineous marriages and the associa... more The aim of this study was to determine the prevalence of consanguineous marriages and the associated factors in the city of Malatya. Materials and Methods: Thirty cluster survey methodology was used to select the study population. Selecting 13 married women per cluster, a total of 409 women were interviewed at their home. Information on the sociodemographic and fertility characteristics of the women and genetic disorders among children was gathered using a face-to-face questionnaire. Data analysis was done by SPSSWIN 13.0. Results: The overall prevalence of consanguinity was 28.4%, with a mean inbreeding coefficient of 0.01081. The principal type of consanguineous marriage was first-cousin marriages, which account for 74.2% of all consanguineous unions. There was no association between consanguinity and sociodemographic characteristics of the participants. Consanguineous marriages had significantly more spontaneous abortions, infant deaths and children with a genetic disorder (P<0.05). Conclusions: The prevalence of consanguinity and of first-cousin marriages was found to be high in the city of Malatya compared to the studies conducted in the past. Future research on the reasons for and negative outcomes of consanguineous marriages should be conducted.

Journal of Photochemistry and Photobiology B: Biology, 2008

Although a variety of physiological and psychological stressors stimulate a significant increase ... more Although a variety of physiological and psychological stressors stimulate a significant increase in adrenomedullin (ADM) levels, suggesting a regulatory or protective role for ADM in countering the hypothalamo-pituitary-adrenal (HPA) axis activation following these stressors, it is still unknown whether light or darkness stress is involved in the endogenous ADM production systems. This study is aimed to investigate the effects of constant light or darkness for 60 days on ADM level in the plasma of adult male rats. ADM concentrations were assessed before and after the stressors in tail arterial blood by using HPLC. In the both groups, ADM levels greatly increased in the first week and than continued with lesser changes from the control levels. In conclusion, the study showed that keeping the rats in constant darkness and light vicinity for a long time altered ADM synthesis and secretion from the plasma or other tissues.

Endocrine Journal, 2003

It is known that, under stress conditions the hypothalamo-pituitary-adrenal (HPA) axis is stimula... more It is known that, under stress conditions the hypothalamo-pituitary-adrenal (HPA) axis is stimulated and catecholamine production is increased. Adrenomedullin (ADM) is a novel peptide that elicits a long-vasorelaxation, and participates in blood pressure regulation via different mechanisms. In the present study, we investigated the administration of ADM on tyrosine hydroxylase (TH) enzyme activity in cold exposed rats. Four groups of Sprague-Dawley rats were studied for their TH enzyme activity in the adrenal medulla and hypothalamus. In addition to measuring blood pressure in these rats, TH enzyme activity in both the adrenal medulla and hypothalamus were examined in four groups of Sprague-Dawley rats: animals exposed to room temperature and cold stress (8ºC, 48 h), and rats injected with ADM (1.0 nmol/kg, i.v.) alone and/or together with cold stress. TH activity was shown to be increased in cold treated groups and decreased in ADM and ADM + cold stress group. Our findings appear to suggest that external ADM application caused an opposite effect on the same system in rats, decreasing the activity of tyrosine hydroxylase (TH) enzyme activity. Furthermore, externally applied ADM was shown to produce its expected hypotensive effect in coldstressed rats. Our results suggest that a possible explanation for the effects of ADM is that, the uptake of ADM under cold stress may effect TH activity in studied tissues.

Acta Biologica Hungarica, 2008

In this work, we investigated whether extended cold exposure increases oxidative damage and susce... more In this work, we investigated whether extended cold exposure increases oxidative damage and susceptibility to oxidants of rat liver, heart, kidney and lung which are metabolically active tissues. Moreover in this study the effect of cold stress on some of the lipid metabolic mediators were studied in rat experimental model. Male albino Sprague-Dawley rats were randomly divided into two groups: The control group (n=12) and the cold-stress group (n=12). Tissue superoxide dismutase (SOD), catalase (CAT), glutathion S-transferase (GST) and glutathion reductase (GR) activities and glutathion (GSH) were measured using standard protocols. The biochemical analyses for total lipid, cholesterol, trigliceride, HDL, VLDL and LDL were done on autoanalyzer. In cold-stress groups SOD activity was decreased in the lung whereas it increased in the heart and kidney. CAT activity was significantly decreased (except liver) in all the tissues in treated rats. GST activity of cold-induced rats increased in liver and heart while decreased in the lung. GR activity was significantly decreased (except in liver) in all the tissues in cold-stressed rats. GSH level was significantly increased in the heart but decreased in the lung of animals exposed to cold when compared to controls. It was found that among the groups trigliceride, total lipid, HDL and VLDL parameters varied significantly but cholesterol and LDL had no significant variance. In this study, we found that exposure of extended (48 h) cold (8 degrees C) caused changes both in the antioxidant defense system (as tissue and enzyme specific) and serum lipoprotein profiles in rats.

Toxicology and industrial health, 2014

Sulfur dioxide (SO2), which is used as food preservative in apricot sulfurization and several fab... more Sulfur dioxide (SO2), which is used as food preservative in apricot sulfurization and several fabricated foods, is a common air pollutant. The aim of this study was to reveal the possible genotoxic effects of SO2 using in vitro human lymphocytes. The different endpoints of genotoxicity: sister chromatid exchange (SCE), micronuclei (MN) tests and cell growth kinetics such as mitotic index (MI) and replication index (RI) were studied. The cells were treated with 0.1, 0.5 and 1.0 ppm concentrations of SO2. It was shown that SO2 caused significant increases in the frequency of SCE and MN in the middle and high dosage groups and also induced mitotic delays and decreased MI and RI. In conclusion, the results have confirmed that SO2 has potent mutagenicity and it can cause genetic damage leading to a malignancy.

Ecological genetics, 2019

Teucrium polium (T. polium) and Rumex crispus (R. crispus) are plant species that grow widely in ... more Teucrium polium (T. polium) and Rumex crispus (R. crispus) are plant species that grow widely in Anatolia and are thought to have healing effects for many diseases. In this study plant extracts are suggested as alternative agents in repairing cellular damage by using sister chromatid exchange (SCE), micronucleus (MN), mitotic index (MI), replication index (RI) and nuclear abnormalities (NAs), against the genotoxicity of cyclophosphamide (CP) in the human lymphocyte cells. 8 experimental groups were formed in the study. The cell culture medium was supplemented with 0.16 μg/ml CP and the cells were treated with 50, 100 and 250 μM T. polium and R. crispus extracts in the presence and absence of CP. As a result, CP significantly decreased MI frequency while increasing SCE, MN and NAs frequencies in cells. 100 μM T. polium plus CP decreased SCEs when compared with CP alone. In addition, MN frequency was significantly decreased in 100 μM T. poliumplus CP and 250 μM R. crispus plus CP comb...

Journal of Assisted Reproduction and Genetics, 2019

Purpose It is known that sperm preparation techniques in in vitro fertilisation (IVF) are intende... more Purpose It is known that sperm preparation techniques in in vitro fertilisation (IVF) are intended to select the best-quality sperm. The aim of this study is to compare sperm the density gradient method and microfluidic chip (Fertile Plus) method in infertile patients by analysing fertilisation rates, pregnancy rates, and sperm morphology and DNA fragmentation rates posed by these two methods. Methods Using semen samples obtained from the patients, sperms were prepared with gradient (n = 312) and microfluidic chip methods (n = 116). Fertilisation and pregnancy rates were compared in the first time and in the recurrent IVF trial patients. In addition, the morphology and DNA fragmentation comparison of sperm samples were evaluated by Toluidine blue in situ chemical staining method. Results There was no statistically significant difference between fertilisation and pregnancy rates when compared with study groups in first-time IVF treatment patients. However, in recurrent IVF failure patients, there was a significant difference in fertilisation rates but no statistically significant difference was found in pregnancy rates. The microfluidic chip method significantly decreased sperm DNA fragmentation index according to density gradient method. Conclusions Microfluidic chip method may be recommended in patients with recurrent unsuccessful in vitro trials. The sperm DNA fragmentation test prior to the treatment will be helpful in selecting the appropriate sperm-washing method.

Sexual Development, 2018

In this case study, we describe a 3-year-old boy who was referred to the Inonu University Hospita... more In this case study, we describe a 3-year-old boy who was referred to the Inonu University Hospital with short stature complaint. His height was 86 cm (-2.96 SDS), weight was 12 kg (-2.43 SDS), and head circumference was 46.5 cm (-2.34 SDS). Chromosomal analyses were performed on cultured peripheral blood lymphocytes of the patient and his parents and showed the patient's karyotype mos 45,X[20]/46,X,idic(Y)(p11.32)[29]/46,XY[1]. The karyotypes of the parents were normal. Subsequently, specific FISH probes were hybridized to the related regions of the sex-determining region Y (SRY), centromere X/Y (CEP X/Y), and short stature homeobox (SHOX) genes. Simultaneous SNP array-CGH was conducted. As to our knowledge, we present the first patient with mosaic isodicentric Y chromosome with 3 different cell lines and normal male external genitalia. Our results suggest that it would be beneficial to study cytogenetic and molecular cytogenetic methods together for better diagnostic accuracy and treatment.