Şener Tulumoğlu - Academia.edu (original) (raw)
Papers by Şener Tulumoğlu
Food Control, 2006
The main purpose of this study was to obtain antimicrobial starter cultures for sourdough process... more The main purpose of this study was to obtain antimicrobial starter cultures for sourdough processes. For this purpose, 60 sourdough samples were collected from different bakery plants in Us ßak. The chemical and microbiological properties of these samples were analyzed. Lactic acid bacteria strains having antimicrobial activity were isolated and identified. The metabolic properties (total amount of acids, organic acids and diacetyl produced and the properties of proteolytic and amylolytic enzymes activity) of the selected strains were determined. The strains with best potential as sourdough starters were L. brevis ssp. lindneri 2103, L. viridenscens 241, 242, Pediococcus sp. E5 and L. delbrueckii F5.
In this study, the probiotic characteristics of Lactobacillus which were taken from the stool sam... more In this study, the probiotic characteristics of Lactobacillus which were taken from the stool samples of 30 children, aged between 5 and 15 years, were studied. The stomach medium (low pH) and bile salt tolerance, bile salt hydrolysis activity, antagonistic activity and cholesterol assimilation quantity of Lactobacillus strains were determined. It was defined that on the whole Lactobacillus strains were resistant to high acidity (pH 2.0–2.5) and sensitive to high levels of bile salt (% 1.00 Oxgall), there was no bile salt hydrolysis activity, they were resistant to vancomycin, teicoplanin and bacitracin and had antagonistic effects on pathogenic bacteria. It was noted that only L. curvatus L26 strain, which produced bacteriocin, had a general antagonistic effect; was indulgent to gastric acid and bile salt; and in different bile salt medium assimilated cholesterol at a high degree (15.22–25.42 µg/ml). At the same time, the fact that L. curvatus L26 had ß-glucuronidase digesting preb...
Acta Physica Polonica A, 2016
In this study, siderophore-producing bacterial species have been isolated from soils contaminated... more In this study, siderophore-producing bacterial species have been isolated from soils contaminated with diesel oil. In P. aeruginosa (P-7) production of siderophores was inhibited at concentrations of ZnSO4 and CoCl2 of 2000 µM, while B. subtilis (BS-1) has increased siderophores production at similar concentrations of CoCl2 and ZnSO4. The same bacteria have increased the production of siderophores in presence of streptomycine (512 mg/ml). In B. subtilis the production of siderophores was inhibited by cefuroxime. The results show the effect of metals on the efficiency of siderophore production by bacteria for potential application in bioremediation of metalcontaminated iron-deficient soils in the microbial assisted phytoremediation processes. The results include the effect of metals on siderophore production efficiency of the bacteria used for the bioremediation of metal-contaminated soils. In conclusion, it has been found that heavy metals and antibiotics significantly effect the production of siderophores by bacteria.
Istanbul University - DergiPark, Jun 15, 2007
Anaerobe, 2013
In the present research, the 20 lactobacilli isolated from children feces aged 4e15 years old wer... more In the present research, the 20 lactobacilli isolated from children feces aged 4e15 years old were investigated for their capabilities to survive at pH 2.0, 2.5, 3.0 and in the presence of 0.25, 0.50 and 0.75% bile salts, their effect on the growth of pathogens, in addition to their sensitivity against 13 selected antibiotics. All the lactobacilli strains were able to survive in low pH and bile salt conditions at pH 2.0 and 0.25% bile salt for 2 h. Moreover, all lactobacilli strains exhibited inhibitory activity against Escherichia coli ATCC 11229, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 29213. In addition, all lactobacilli strains indicated resistance to teicoplanin, vancomycin, and bacitracin. The amount of exopolysaccharide (EPS) produced by the strains was 70 and 290 mg/L. The capabilities to autoaggregation and coaggregate with E. coli ATCC 11229 of the strains were also evaluated. High EPS-producing strains indicated significant autoaggregation and coaggregation capability with test bacteria (p < 0.01). The maximum cholesterol removal (76.5%) was observed by strain Lactobacillus pentosus T3, producing a high amount of exopolysaccharide, in 0.3%oxgall concentration (p < 0.05). Our results demonstrate that the capability to EPS production, acid-bile tolerance, antimicrobial activity, antibiotic resistance, aggregation and cholesterol removal of lactobacilli could be utilized for preliminary screening in order to identify potentially probiotic bacteria suitable for human.
Many studies have shown that Lactobacillus species are bacteria that are mostly used as probiotic... more Many studies have shown that Lactobacillus species are bacteria that are mostly used as probiotics. Here, we evaluated cholesterol assimilation and folic acid production by determining the probiotic properties of Lactobacillus spp from raw goat milk, which has prebiotic properties. Bacteria were isolated from goat milk, and API 50 CHL and 16sRNA identification was performed. Probiotic properties were determined based on bile salt and acidic tolerance, hydrophobicity, haemolytic activity, antibiotic susceptibility, antagonistic effect, and exopolysaccharide production. In addition, cholesterol assimilation and folate production of cultures were determined. The exopolysaccharide production of Lactobacillus plantarum GM-12 was moderate (++), with the highest folate production (179 ng /dl), and high cholesterol assimilation (60 mg / dl). The exopolysaccharide production of L. plantarum GM-15 was high (+++), with high folate production (160 ng / dl) and high cholesterol assimilation (58 ...
Mediterranean Journal of Hematology and Infectious Diseases, 2014
Background: Tuberculosis(TB) remains a major global health problem. The childhood tuberculosis ha... more Background: Tuberculosis(TB) remains a major global health problem. The childhood tuberculosis has some unique features different which makes the diagnosis more complicated. We described the epidemiologic, clinical and microbiologic features of children with extrapulmonary and pulmonary TB. Methods: The data of the patients <17 years with active TB were collected and compared in pulmonary(PTB) and extrapulmonary TB(EXPTB) patients.Results: A total of 128 cases were included. Fourty two cases occurred in children were < 5 years of age; 41 cases between 6-10 years and 45 cases > 10 years. PTB was present in 75.0% of the cases and EXPTB was present in 25% of cases. There was no significant difference between the EXPTB and PTB by means of distribution of age groups(p=0,201). The rate of patients free of constitutional symptoms were significantly higher in EXPTB compared to PTB(p=0.000). There was no significant difference between EXPTB and PTB by means of sources detec...
Bu çalışmanın amacı çeşitli klinik örneklerden izole edilen 101 maya suşunun tür tanımlanması ve ... more Bu çalışmanın amacı çeşitli klinik örneklerden izole edilen 101 maya suşunun tür tanımlanması ve antifungal duyarlıklarının belirlenmesidir. İzolatların identifikasyonu API 20 C AUX (Biomérieux, France) kiti ile yapılmış olup, API ATB (Biomérieux, France) fungus kiti ile suşların flusitozin, amfoterisin B, flukonazol, itrakonazol ve vorikonazola karşı antifungal duyarlılıkları belirlenmiştir. Yüz bir klinik suşun 48’i idrar, 46’sı kan, 5’i kateter ve 2’si yaradan izole edildi. Suşların türlere göre dağılımında en sık C. albicans (54.46%), ikinci sıklıkta C. parapsilosis (33.66%), üçüncü sıklıkta C. famata (6.93%), dördüncü sıklıkta C. tropicalis (2.97%) ve beşinci sıklıkta C. pelliculosa (1.98%) izledi. C. albicans suşlarında vorikonazol ve flukonazola karşı direnç oranları sırasıyla %5.45 ve % 3.63 olarak saptandı. Diğer Candida suşlarında ise özellikle itrakonazola karşı farklı oranlarda direnç saptandı. Candida infeksiyonlarının etkin tedavisi için antifungal duyarlılığın ve tür ...
Foundations of Evidence-Based Medicine, 2003
International Current Pharmaceutical Journal, 2016
In this study, the effect of the incubation time, incubation temperature, pH, metal and sodium io... more In this study, the effect of the incubation time, incubation temperature, pH, metal and sodium ions on the antibacterial activity of chitosan against food borne spoilage bacteria focused. Aeromonas hydrophila and Staphylococcus aureus, were used as two food borne bacteria. Acetic acid was used dissolving of chitosan. Results showed that chitosan solution at 5 mg/mL significantly inhibited the growth of A. hydrophila and S. aureus in presence of time, pH and metal ions (p <0.01). However, temperature did not significantly affect the antibacterial activity (4°C, 25°C and 37°C) (p >0.01). Chitosan activity increased with increasing incubation time. The influence of below pH 6 on the antibacterial activity of chitosan was significantly influenced (p<0.01). The addition of Ba +2 reduced chitosan activity against A. hydrophila while the addition of Ca +2 have increased chitosan activity against S. aureus. Sodium ions at a concentration of 25 mM reduced chitosan activity against S. aureus. These results show that chitosan can be used food industry to prevent the development of spoilage bacteria.
Mediterranean Journal of Hematology and Infectious Diseases, 2014
Introduction: Tuberculosis (TB) remains a major global health problem affecting millions of peopl... more Introduction: Tuberculosis (TB) remains a major global health problem affecting millions of people annually. Tuberculosis in children has unique features different from adults which makes the diagnosis to be more difficult. The spectrum of the symptoms of TB in children could vary from non-specific symptoms to severe clinical presentations. In this study, we reviewed our experience at pediatric patients with active TB admitted in a tertiary hospital and aimed to compare the epidemiological, clinical and microbiological features of children with the extra-pulmonary tuberculosis(EPTB) and pulmonary tuberculosis(PTB).Material and methods: Patients under 14 years of age diagnosed with active TB in our unit between December 2008 and September 2013 were included in the study. Data including demographic characteristics, clinical history, microbiology, imaging studies, medications and outcomes of the patients were collected from medical records.Results: A total of 129 cases of active T...
Anaerobe, 2014
The aim of this study was to characterize the probiotic characteristics of Lactobacillus fermentu... more The aim of this study was to characterize the probiotic characteristics of Lactobacillus fermentum strains isolated from Tulum cheese. Seven L. fermentum strains were selected among the isolated and identified lactobacillus strains due to their abundance. When the gastric condition was considered, L. fermentum LP3 and LP4 were able to tolerate pH 2.5 and 1% bile salt. All L. fermentum strains had similar enzymatic activity and antibiotic resistance pattern but the highest antagonistic effect was detected within LP3, LP4 and LP6. Cholesterol assimilation amount of L. fermentum strains ranged between 12.1 and 45.3% in MRS and 20.7-71.1% in MRS with bile. The highest cholesterol assimilation in MRS and MRS with bile was occurred by LP3 and LP4, respectively. L. fermentum LP2 adhered to caco-2 cells more than Lactobacillus rhamnosus LGG where LP3, LP4 and LP5 adhered at similar level. In conclusion, L. fermentum LP3 and LP4 fulfilled sufficient criteria to be probiotics for use as a starter culture in the production of tulum cheese or other dairy products. Also this study indicated that some food-associated Lactobacillus strains non-predominant for gut biota have significant probiotic potential.
Pediatric Hematology and Oncology, 2014
Pediatric cancer patients have an increased risk of potentially life-threatening fungal infection... more Pediatric cancer patients have an increased risk of potentially life-threatening fungal infections such as Candida parapsilosis, associated with long-term CVADs. The Infectious Diseases Society of America (IDSA) guidelines on Candida catheter-related bloodstream infections recommend systemic antifungal therapy and catheter removal. In this study, we focused on our experience with antifungal failure due to totally implanted catheter-associated C. parapsilosis bloodstream infections. We investigated cases leading to port removal in pediatric malignancy patients and the associated patient outcomes. In the first phase of the study, a retrospective chart review was performed to collect patient information, including primary disease; time from hospitalization to port-related candidemia; antifungal drug choice; and the time at which port removal occurred. During the second phase, antifungal susceptibility tests for C. parapsilosis were performed in our microbiology laboratory. All patients had fevers and were neutropenic at the time of candidemia diagnosis. The mean duration between the first isolation of Candida parapsilosis from the port samples to the port removal was 9.75 ± 5.29 days for 11 patients. Patient fevers lasted for a mean time of 16.22 ± 6.51 days. The median recovery duration from fever after CVC removal was four days (range 2-12 days). The median duration for achieving negative blood cultures, following antifungal treatment was 18 days (range 10-27 days). Our data favored the removal of catheters in the presence of ongoing fever, as suggested by the guidelines, independent of the chosen antifungal treatment. Future studies with large samples are needed to evaluate the effects of catheter removal on mortality rates and patient outcomes.
Mycopathologia, 2011
Candida ciferrii, which is known as an agent of superficial yeast infection and onychomycosis, ha... more Candida ciferrii, which is known as an agent of superficial yeast infection and onychomycosis, has rarely been isolated as an agent of candidemia. Limited reports have suggested different patterns of antifungal sensitivity. We report a rare candidemia case caused by c.ciferrii in an 8-year-old child in which isolated candida species were resistant to amphotericin-B (MIC &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; 1 μg/ml), fluconazole, (MIC ≥ 64 μg/ml), caspofungin (MIC ≥ 32 μg/ml), and anidulafungin (MIC ≥ 32 μg/ml) but sensitive to voriconazole (MIC ≤ 0.12 μg/ml). As far as we aware, this was the first recorded C. ciferrii candidemia case in children.
The aim of this study was to evaluate the public health significance of representative motile Aer... more The aim of this study was to evaluate the public health significance of representative motile Aeromonas isolates, which were mainly isolated from different foods, and to determine the effects of different temperatures on virulence factors such as hemolysin, protease, lipase and nuclease. The influence of different temperatures (+4 °C, +25 °C and +37 °C) on virulence factors of 40 isolates of Aeromonas (A.) spp. (A. hydrophila, A. caviae and A. sobria) was investigated. All isolates could produce hemolysin, protease, lipase, and nuclease at +37 °C, +25 °C and +4 °C; however, these virulence associated factors were produced faster at +37 °C and +25 °C than at +4 °C. All Aeromonas species were also tested for antibiotic resistance patterns and were found to be resistant to ampicillin, yet sensitive to imipenem, ciprofloxacin and amikacin antibiotics.
Kafkas Universitesi Veteriner Fakultesi Dergisi, 2009
The purpose of this study was to identify and determine antifungal susceptibility patterns of 101... more The purpose of this study was to identify and determine antifungal susceptibility patterns of 101 yeast strains isolated from varios clinical specimens. Identification of the isolates were determined by API 20 C AUX kit (Biomerieux, France) and antifungal susceptibilities of these species to flucytosine, amphotericin B, fluconazole, itraconazole and voriconazole were determined by API ATB Fungus kit (Biomerieux, France). Of the 101 strains, 48 were isolated from urine, 46 from blood, 5 from catheter and 2 from wound. C.albicans (54.46%) was the most frequently isolated species, followed by C.parapsilosis (33.66%), C.famata (6.93%), C.tropicalis (2.97%) and C.pelliculosa (1.98%). High rates of resistance against itraconazole, fluconazole and voricanozole were detected in C.albicans. Different rates of resistance against especially itraconazole were detected in other Candida strains. Species definition and determination of antifungal susceptibility patterns are advised for the proper ...
Gida the Journal of Food, 1991
Peyniralti suyu tozundan alti farkli sentetik besi ortami hazirlanmistir. Bu besi ortamlarinda 12... more Peyniralti suyu tozundan alti farkli sentetik besi ortami hazirlanmistir. Bu besi ortamlarinda 12 adet L. bulgaricus susunun olusturdugu laktik asit miktarlari tespit edilmistir. Katkili besi ortamlarinda olusan laktik asit miktarlari, katkisiz besi ortamlari ile kiyaslandiginda daha fazla bulunmustur. 12 adet L. bulgaricus susu arasinda en fazla laktik asit uretenlerin L. bulgaricus B1, L. bulgaricus B4 ve L. bulgaricus B11 suslari oldugu gorulmustur.
Journal of Pediatric Infection
Food Control, 2006
The main purpose of this study was to obtain antimicrobial starter cultures for sourdough process... more The main purpose of this study was to obtain antimicrobial starter cultures for sourdough processes. For this purpose, 60 sourdough samples were collected from different bakery plants in Us ßak. The chemical and microbiological properties of these samples were analyzed. Lactic acid bacteria strains having antimicrobial activity were isolated and identified. The metabolic properties (total amount of acids, organic acids and diacetyl produced and the properties of proteolytic and amylolytic enzymes activity) of the selected strains were determined. The strains with best potential as sourdough starters were L. brevis ssp. lindneri 2103, L. viridenscens 241, 242, Pediococcus sp. E5 and L. delbrueckii F5.
In this study, the probiotic characteristics of Lactobacillus which were taken from the stool sam... more In this study, the probiotic characteristics of Lactobacillus which were taken from the stool samples of 30 children, aged between 5 and 15 years, were studied. The stomach medium (low pH) and bile salt tolerance, bile salt hydrolysis activity, antagonistic activity and cholesterol assimilation quantity of Lactobacillus strains were determined. It was defined that on the whole Lactobacillus strains were resistant to high acidity (pH 2.0–2.5) and sensitive to high levels of bile salt (% 1.00 Oxgall), there was no bile salt hydrolysis activity, they were resistant to vancomycin, teicoplanin and bacitracin and had antagonistic effects on pathogenic bacteria. It was noted that only L. curvatus L26 strain, which produced bacteriocin, had a general antagonistic effect; was indulgent to gastric acid and bile salt; and in different bile salt medium assimilated cholesterol at a high degree (15.22–25.42 µg/ml). At the same time, the fact that L. curvatus L26 had ß-glucuronidase digesting preb...
Acta Physica Polonica A, 2016
In this study, siderophore-producing bacterial species have been isolated from soils contaminated... more In this study, siderophore-producing bacterial species have been isolated from soils contaminated with diesel oil. In P. aeruginosa (P-7) production of siderophores was inhibited at concentrations of ZnSO4 and CoCl2 of 2000 µM, while B. subtilis (BS-1) has increased siderophores production at similar concentrations of CoCl2 and ZnSO4. The same bacteria have increased the production of siderophores in presence of streptomycine (512 mg/ml). In B. subtilis the production of siderophores was inhibited by cefuroxime. The results show the effect of metals on the efficiency of siderophore production by bacteria for potential application in bioremediation of metalcontaminated iron-deficient soils in the microbial assisted phytoremediation processes. The results include the effect of metals on siderophore production efficiency of the bacteria used for the bioremediation of metal-contaminated soils. In conclusion, it has been found that heavy metals and antibiotics significantly effect the production of siderophores by bacteria.
Istanbul University - DergiPark, Jun 15, 2007
Anaerobe, 2013
In the present research, the 20 lactobacilli isolated from children feces aged 4e15 years old wer... more In the present research, the 20 lactobacilli isolated from children feces aged 4e15 years old were investigated for their capabilities to survive at pH 2.0, 2.5, 3.0 and in the presence of 0.25, 0.50 and 0.75% bile salts, their effect on the growth of pathogens, in addition to their sensitivity against 13 selected antibiotics. All the lactobacilli strains were able to survive in low pH and bile salt conditions at pH 2.0 and 0.25% bile salt for 2 h. Moreover, all lactobacilli strains exhibited inhibitory activity against Escherichia coli ATCC 11229, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 29213. In addition, all lactobacilli strains indicated resistance to teicoplanin, vancomycin, and bacitracin. The amount of exopolysaccharide (EPS) produced by the strains was 70 and 290 mg/L. The capabilities to autoaggregation and coaggregate with E. coli ATCC 11229 of the strains were also evaluated. High EPS-producing strains indicated significant autoaggregation and coaggregation capability with test bacteria (p < 0.01). The maximum cholesterol removal (76.5%) was observed by strain Lactobacillus pentosus T3, producing a high amount of exopolysaccharide, in 0.3%oxgall concentration (p < 0.05). Our results demonstrate that the capability to EPS production, acid-bile tolerance, antimicrobial activity, antibiotic resistance, aggregation and cholesterol removal of lactobacilli could be utilized for preliminary screening in order to identify potentially probiotic bacteria suitable for human.
Many studies have shown that Lactobacillus species are bacteria that are mostly used as probiotic... more Many studies have shown that Lactobacillus species are bacteria that are mostly used as probiotics. Here, we evaluated cholesterol assimilation and folic acid production by determining the probiotic properties of Lactobacillus spp from raw goat milk, which has prebiotic properties. Bacteria were isolated from goat milk, and API 50 CHL and 16sRNA identification was performed. Probiotic properties were determined based on bile salt and acidic tolerance, hydrophobicity, haemolytic activity, antibiotic susceptibility, antagonistic effect, and exopolysaccharide production. In addition, cholesterol assimilation and folate production of cultures were determined. The exopolysaccharide production of Lactobacillus plantarum GM-12 was moderate (++), with the highest folate production (179 ng /dl), and high cholesterol assimilation (60 mg / dl). The exopolysaccharide production of L. plantarum GM-15 was high (+++), with high folate production (160 ng / dl) and high cholesterol assimilation (58 ...
Mediterranean Journal of Hematology and Infectious Diseases, 2014
Background: Tuberculosis(TB) remains a major global health problem. The childhood tuberculosis ha... more Background: Tuberculosis(TB) remains a major global health problem. The childhood tuberculosis has some unique features different which makes the diagnosis more complicated. We described the epidemiologic, clinical and microbiologic features of children with extrapulmonary and pulmonary TB. Methods: The data of the patients <17 years with active TB were collected and compared in pulmonary(PTB) and extrapulmonary TB(EXPTB) patients.Results: A total of 128 cases were included. Fourty two cases occurred in children were < 5 years of age; 41 cases between 6-10 years and 45 cases > 10 years. PTB was present in 75.0% of the cases and EXPTB was present in 25% of cases. There was no significant difference between the EXPTB and PTB by means of distribution of age groups(p=0,201). The rate of patients free of constitutional symptoms were significantly higher in EXPTB compared to PTB(p=0.000). There was no significant difference between EXPTB and PTB by means of sources detec...
Bu çalışmanın amacı çeşitli klinik örneklerden izole edilen 101 maya suşunun tür tanımlanması ve ... more Bu çalışmanın amacı çeşitli klinik örneklerden izole edilen 101 maya suşunun tür tanımlanması ve antifungal duyarlıklarının belirlenmesidir. İzolatların identifikasyonu API 20 C AUX (Biomérieux, France) kiti ile yapılmış olup, API ATB (Biomérieux, France) fungus kiti ile suşların flusitozin, amfoterisin B, flukonazol, itrakonazol ve vorikonazola karşı antifungal duyarlılıkları belirlenmiştir. Yüz bir klinik suşun 48’i idrar, 46’sı kan, 5’i kateter ve 2’si yaradan izole edildi. Suşların türlere göre dağılımında en sık C. albicans (54.46%), ikinci sıklıkta C. parapsilosis (33.66%), üçüncü sıklıkta C. famata (6.93%), dördüncü sıklıkta C. tropicalis (2.97%) ve beşinci sıklıkta C. pelliculosa (1.98%) izledi. C. albicans suşlarında vorikonazol ve flukonazola karşı direnç oranları sırasıyla %5.45 ve % 3.63 olarak saptandı. Diğer Candida suşlarında ise özellikle itrakonazola karşı farklı oranlarda direnç saptandı. Candida infeksiyonlarının etkin tedavisi için antifungal duyarlılığın ve tür ...
Foundations of Evidence-Based Medicine, 2003
International Current Pharmaceutical Journal, 2016
In this study, the effect of the incubation time, incubation temperature, pH, metal and sodium io... more In this study, the effect of the incubation time, incubation temperature, pH, metal and sodium ions on the antibacterial activity of chitosan against food borne spoilage bacteria focused. Aeromonas hydrophila and Staphylococcus aureus, were used as two food borne bacteria. Acetic acid was used dissolving of chitosan. Results showed that chitosan solution at 5 mg/mL significantly inhibited the growth of A. hydrophila and S. aureus in presence of time, pH and metal ions (p <0.01). However, temperature did not significantly affect the antibacterial activity (4°C, 25°C and 37°C) (p >0.01). Chitosan activity increased with increasing incubation time. The influence of below pH 6 on the antibacterial activity of chitosan was significantly influenced (p<0.01). The addition of Ba +2 reduced chitosan activity against A. hydrophila while the addition of Ca +2 have increased chitosan activity against S. aureus. Sodium ions at a concentration of 25 mM reduced chitosan activity against S. aureus. These results show that chitosan can be used food industry to prevent the development of spoilage bacteria.
Mediterranean Journal of Hematology and Infectious Diseases, 2014
Introduction: Tuberculosis (TB) remains a major global health problem affecting millions of peopl... more Introduction: Tuberculosis (TB) remains a major global health problem affecting millions of people annually. Tuberculosis in children has unique features different from adults which makes the diagnosis to be more difficult. The spectrum of the symptoms of TB in children could vary from non-specific symptoms to severe clinical presentations. In this study, we reviewed our experience at pediatric patients with active TB admitted in a tertiary hospital and aimed to compare the epidemiological, clinical and microbiological features of children with the extra-pulmonary tuberculosis(EPTB) and pulmonary tuberculosis(PTB).Material and methods: Patients under 14 years of age diagnosed with active TB in our unit between December 2008 and September 2013 were included in the study. Data including demographic characteristics, clinical history, microbiology, imaging studies, medications and outcomes of the patients were collected from medical records.Results: A total of 129 cases of active T...
Anaerobe, 2014
The aim of this study was to characterize the probiotic characteristics of Lactobacillus fermentu... more The aim of this study was to characterize the probiotic characteristics of Lactobacillus fermentum strains isolated from Tulum cheese. Seven L. fermentum strains were selected among the isolated and identified lactobacillus strains due to their abundance. When the gastric condition was considered, L. fermentum LP3 and LP4 were able to tolerate pH 2.5 and 1% bile salt. All L. fermentum strains had similar enzymatic activity and antibiotic resistance pattern but the highest antagonistic effect was detected within LP3, LP4 and LP6. Cholesterol assimilation amount of L. fermentum strains ranged between 12.1 and 45.3% in MRS and 20.7-71.1% in MRS with bile. The highest cholesterol assimilation in MRS and MRS with bile was occurred by LP3 and LP4, respectively. L. fermentum LP2 adhered to caco-2 cells more than Lactobacillus rhamnosus LGG where LP3, LP4 and LP5 adhered at similar level. In conclusion, L. fermentum LP3 and LP4 fulfilled sufficient criteria to be probiotics for use as a starter culture in the production of tulum cheese or other dairy products. Also this study indicated that some food-associated Lactobacillus strains non-predominant for gut biota have significant probiotic potential.
Pediatric Hematology and Oncology, 2014
Pediatric cancer patients have an increased risk of potentially life-threatening fungal infection... more Pediatric cancer patients have an increased risk of potentially life-threatening fungal infections such as Candida parapsilosis, associated with long-term CVADs. The Infectious Diseases Society of America (IDSA) guidelines on Candida catheter-related bloodstream infections recommend systemic antifungal therapy and catheter removal. In this study, we focused on our experience with antifungal failure due to totally implanted catheter-associated C. parapsilosis bloodstream infections. We investigated cases leading to port removal in pediatric malignancy patients and the associated patient outcomes. In the first phase of the study, a retrospective chart review was performed to collect patient information, including primary disease; time from hospitalization to port-related candidemia; antifungal drug choice; and the time at which port removal occurred. During the second phase, antifungal susceptibility tests for C. parapsilosis were performed in our microbiology laboratory. All patients had fevers and were neutropenic at the time of candidemia diagnosis. The mean duration between the first isolation of Candida parapsilosis from the port samples to the port removal was 9.75 ± 5.29 days for 11 patients. Patient fevers lasted for a mean time of 16.22 ± 6.51 days. The median recovery duration from fever after CVC removal was four days (range 2-12 days). The median duration for achieving negative blood cultures, following antifungal treatment was 18 days (range 10-27 days). Our data favored the removal of catheters in the presence of ongoing fever, as suggested by the guidelines, independent of the chosen antifungal treatment. Future studies with large samples are needed to evaluate the effects of catheter removal on mortality rates and patient outcomes.
Mycopathologia, 2011
Candida ciferrii, which is known as an agent of superficial yeast infection and onychomycosis, ha... more Candida ciferrii, which is known as an agent of superficial yeast infection and onychomycosis, has rarely been isolated as an agent of candidemia. Limited reports have suggested different patterns of antifungal sensitivity. We report a rare candidemia case caused by c.ciferrii in an 8-year-old child in which isolated candida species were resistant to amphotericin-B (MIC &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; 1 μg/ml), fluconazole, (MIC ≥ 64 μg/ml), caspofungin (MIC ≥ 32 μg/ml), and anidulafungin (MIC ≥ 32 μg/ml) but sensitive to voriconazole (MIC ≤ 0.12 μg/ml). As far as we aware, this was the first recorded C. ciferrii candidemia case in children.
The aim of this study was to evaluate the public health significance of representative motile Aer... more The aim of this study was to evaluate the public health significance of representative motile Aeromonas isolates, which were mainly isolated from different foods, and to determine the effects of different temperatures on virulence factors such as hemolysin, protease, lipase and nuclease. The influence of different temperatures (+4 °C, +25 °C and +37 °C) on virulence factors of 40 isolates of Aeromonas (A.) spp. (A. hydrophila, A. caviae and A. sobria) was investigated. All isolates could produce hemolysin, protease, lipase, and nuclease at +37 °C, +25 °C and +4 °C; however, these virulence associated factors were produced faster at +37 °C and +25 °C than at +4 °C. All Aeromonas species were also tested for antibiotic resistance patterns and were found to be resistant to ampicillin, yet sensitive to imipenem, ciprofloxacin and amikacin antibiotics.
Kafkas Universitesi Veteriner Fakultesi Dergisi, 2009
The purpose of this study was to identify and determine antifungal susceptibility patterns of 101... more The purpose of this study was to identify and determine antifungal susceptibility patterns of 101 yeast strains isolated from varios clinical specimens. Identification of the isolates were determined by API 20 C AUX kit (Biomerieux, France) and antifungal susceptibilities of these species to flucytosine, amphotericin B, fluconazole, itraconazole and voriconazole were determined by API ATB Fungus kit (Biomerieux, France). Of the 101 strains, 48 were isolated from urine, 46 from blood, 5 from catheter and 2 from wound. C.albicans (54.46%) was the most frequently isolated species, followed by C.parapsilosis (33.66%), C.famata (6.93%), C.tropicalis (2.97%) and C.pelliculosa (1.98%). High rates of resistance against itraconazole, fluconazole and voricanozole were detected in C.albicans. Different rates of resistance against especially itraconazole were detected in other Candida strains. Species definition and determination of antifungal susceptibility patterns are advised for the proper ...
Gida the Journal of Food, 1991
Peyniralti suyu tozundan alti farkli sentetik besi ortami hazirlanmistir. Bu besi ortamlarinda 12... more Peyniralti suyu tozundan alti farkli sentetik besi ortami hazirlanmistir. Bu besi ortamlarinda 12 adet L. bulgaricus susunun olusturdugu laktik asit miktarlari tespit edilmistir. Katkili besi ortamlarinda olusan laktik asit miktarlari, katkisiz besi ortamlari ile kiyaslandiginda daha fazla bulunmustur. 12 adet L. bulgaricus susu arasinda en fazla laktik asit uretenlerin L. bulgaricus B1, L. bulgaricus B4 ve L. bulgaricus B11 suslari oldugu gorulmustur.
Journal of Pediatric Infection