Андрей Чаплин - Academia.edu (original) (raw)
Андрей Чаплин
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National Research Nuclear University MEPhI (Moscow Engineering Physics Institute)
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Вестник Российской академии медицинских наук, 2015
Background: The members of genus Bifidobacterium represent a significant part of intestinal micro... more Background: The members of genus Bifidobacterium represent a significant part of intestinal microbiota in adults and predominate in infants. Species repertoire of the intestinal bifidobacteria is known to be subjected to major changes with age; however, many details of this process are still to be elucidated.Objective: Our aim was to study the diversity of intestinal bifidobacteria and changes of their qualitative and quantitative composition characteristics during the process of growing up using MALDI-TOF mass-spectrometric analysis of pure bacterial cultures.Methods: A cross-sectional study of bifidobacteria in the intestinal microbiota was performed in 93 healthy people of the ages from 1 month to 57 years. Strains were identified using Microflex LT MALDI-TOF MS, the confirmation was performed by 16S rRNA gene fragment sequencing.Results: 93% of isolated bifidobacterial strains were successfully identified using MALDI-TOF mass-spectrometry. At least two of the strains from each s...
Microbes are ubiquitous. We have learned to deal with their pathogenic effects by developing anti... more Microbes are ubiquitous. We have learned to deal with their pathogenic effects by developing antibiotics, antiviral and antimycotic drugs, but they evolve so rapidly that we are sometimes forced to step back. Valentin Makarov of A. N. Belozersky Research Institute of Physico-Chemical Biology MSU and his colleagues discuss the role and prospects of high throughput sequencing in the identification of emerging pathogens. The issue also contains 8 original articles on the evolution of infections.
Вестник Российской академии медицинских наук, 2015
Background: The members of genus Bifidobacterium represent a significant part of intestinal micro... more Background: The members of genus Bifidobacterium represent a significant part of intestinal microbiota in adults and predominate in infants. Species repertoire of the intestinal bifidobacteria is known to be subjected to major changes with age; however, many details of this process are still to be elucidated.Objective: Our aim was to study the diversity of intestinal bifidobacteria and changes of their qualitative and quantitative composition characteristics during the process of growing up using MALDI-TOF mass-spectrometric analysis of pure bacterial cultures.Methods: A cross-sectional study of bifidobacteria in the intestinal microbiota was performed in 93 healthy people of the ages from 1 month to 57 years. Strains were identified using Microflex LT MALDI-TOF MS, the confirmation was performed by 16S rRNA gene fragment sequencing.Results: 93% of isolated bifidobacterial strains were successfully identified using MALDI-TOF mass-spectrometry. At least two of the strains from each s...
Microbes are ubiquitous. We have learned to deal with their pathogenic effects by developing anti... more Microbes are ubiquitous. We have learned to deal with their pathogenic effects by developing antibiotics, antiviral and antimycotic drugs, but they evolve so rapidly that we are sometimes forced to step back. Valentin Makarov of A. N. Belozersky Research Institute of Physico-Chemical Biology MSU and his colleagues discuss the role and prospects of high throughput sequencing in the identification of emerging pathogens. The issue also contains 8 original articles on the evolution of infections.