Дмитрий Крутило - Academia.edu (original) (raw)
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Papers by Дмитрий Крутило
Ecological genetics, 2013
We have conducted the analysis of sequences of the 16S rRNA gene and intergenic 16S 23S rRNA regi... more We have conducted the analysis of sequences of the 16S rRNA gene and intergenic 16S 23S rRNA region (ITS) of nodule bacteria of soybean which differ in growth speed and are isolated from soils of Ukraine with different growth rate intensities. As a result we have shown the similarity of strains with an intense growth rate to soybean rhizobia of group USDA 123. The studied strains, as other representatives of this group, pos sess increased saprophytic competence. The restriction analysis divided all sequences of the intergenic ITS region of soybean rhizobia into two ITS types: the first ITS type includes strains with an intensive growth rate and the second ITS type contains strains with a slow grow rate. These types also coincide with their physio logical groups.
Mikrobiolohichnyi Zhurnal
Agriciltural microbiology, 2016
In the vegetation experiment conditions it was established that the greatest effect of soybean ba... more In the vegetation experiment conditions it was established that the greatest effect of soybean bacterization can be obtained by two strains of nodule bacteria with slow (Bradyrhizobium japonicum 46) and intensive (B. japonicum КВ11) growth rates when these strains used in inoculum in the ratio 1 : 1 (binary composition). In compared to the mono-inoculation the combined use of these strains contributed the formation of balanced symbiotic system, increase the level of molecular nitrogen fixation, content of chlorophyll in the biomass leaves and above ground mass of different soybean plants varieties on 7.8–19.6 %. In a field experiment inoculation of soybean seeds with a composition of B. japonicum strains to increase the soybean yields by 11.1–13.7 % compared to the mono-inoculation.
Aim. To investigate the genetic diversity of soybean nodule bacteria, widespread in agrocenoses o... more Aim. To investigate the genetic diversity of soybean nodule bacteria, widespread in agrocenoses of Ukraine. Methods. Nodule bacteria were isolated from soybean nodules grown in soil samples selected in different regions of Ukraine. The amplification of the intergenic region 16S-23S rRNA (ITS-region) was conducted with the use of primers FGPS1490-72 and FGPL132-38. For PCR-products restriction there were applied restriction endonucleases MspI, HaeIII and NdeII. Results. The diversity of Bradyrhizobium japonicum strains with different growthrates was investigated using PCR-RFLP method. According to analysis of 16S-23S rDNA with enzymes MspI, HaeIII and NdeII it was obtained that all the intensivegrowing strains had the same restriction profiles, so they were combined into a single genetic group. Based on ITS-region restriction the slow-growing strains were assigned into two genetic groups using MspI enzyme, three genetic groups using HaeIII and four genomogroups using NdeII enzyme. Сo...
Проведён анализ последовательностей гена 16s рРнк и межгенного региона 16s-23s рРнк (ITS) клубен... more Проведён анализ последовательностей гена 16s рРнк и межгенного региона 16s-23s рРнк (ITS) клубеньковых
бактерий сои, отличающихся скоростью роста и выделенных
из почв Украины с различной интенсивностью выращивания
данной культуры. В результате показана близость штаммов
с интенсивным ростом к ризобиям сои группы USDA 123.
исследуемые штаммы, как и другие представители этой
группы, обладают повышенной сапрофитной компетентностью.
рестрикционный анализ последовательностей межгенного
региона ITS ризобий сои позволил разделить их на два ITS-типа:
первый ITS-тип — штаммы с интенсивным ростом и второй ITS-тип — медленнорастущие штаммы. такое разделение штаммов cоответствует распределению их на физиологические группы.
Із ґрунтів різних регіонів України вилучено 50 штамів бульбочкових бактерій квасолі та вивчено їх... more Із ґрунтів різних регіонів України вилучено 50 штамів бульбочкових бактерій квасолі та вивчено їх біологічне різноманіття. Встановлено, що нові штами Rhizobium phaseoli істотно відрізняються за чутливістю до антибіотиків, серологічними та генетичними властивостями. За антигенним складом досліджені бульбочкові бактерії віднесено до різних серологічних груп. Із застосуванням методу ПЛР-RFLP IТS-регіону виявлено значний генетичний поліморфізм мікросимбіонтів квасолі, виділених із ґрунтових популяцій ризобій. За рестрикційними профілями 16S-23S рДНК їх вперше розділено на різні IТS-типи.
Aim. To study genetic properties of soybean nodule bacteria with different growth rates, to resea... more Aim. To study genetic properties of soybean nodule bacteria with different growth rates, to research the ability of rhizobia to produce phytohormonal substances and estimate their symbiotic potential in the conditions of combined inoculation and mono-inoculation of soybean plants. Methods. Sequencing of the 16S-23S rDNA intergenic spacer of soybean rhizobia was performed on ABI 3130xl Genetic Analyzer. Determination of the qualitative and quantitative composition of phytohormones in culture liquid of Bradyrhizobium ja-ponicum strains was performed by SDTLC-chromatography of high resolution. Symbiotic potential of the strains was estimated in the field experiment in Polissia area of Ukraine. Results. It has been established that strains of soybean nodule bacteria with different growth rates by the structure of ITS region belong to different genetic groups: USDA 6, USDA 110 and USDA 123. The studied rhizobia differ in the levels of extracellular phy-tohormones synthesis: the slow-growing B. japonicum strain 46 produces more amounts of auxins, but the intensive-growing B. japonicum strain KB11 significantly exceeds in the amounts of cytokinins. It has been shown that an important factor of increasing soybean productivity is the formation of balanced symbiotic systems of the host plant with several complementary strains but serologically and genetically different strains of rhizobia of the same species. Conclusions. Combined inoculation of soybean seeds with two active strains B. japonicum 46 and B. japonicum KB11 allows more fully realizing the potential of symbiotic partners and receiving the higher yield. K e y w o r d s: Bradyrhizobium japonicum, soybean, 16S-23S rDNA (
In the vegetation experiment conditions it was established that the greatest effect of soybean ba... more In the vegetation experiment conditions it was established that the greatest effect of soybean bacterization can be obtained by two strains of nodule bacteria with slow (Bradyrhizobium japonicum 46) and intensive (B. japonicum КВ11) growth rates when these strains used in inocu-lum in the ratio 1 : 1 (binary composition). In compared to the mono-inoculation the combined use of these strains contributed the formation of balanced symbiotic system, increase the level of molecular nitrogen fixation, content of chlorophyll in the biomass leaves and above ground mass of different soybean plants varieties on 7.8–19.6 %. In a field experiment inoculation of soybean seeds with a composition of B. japonicum strains to increase the soybean yields by 11.1–13.7 % compared to the mono-inoculation.
We have conducted the analysis of sequences of the 16S rRNA gene and intergenic 16S-23S rRNA regi... more We have conducted the analysis of sequences of the 16S rRNA gene and intergenic 16S-23S rRNA region (ITS) of nodule bacteria of soybean which differ in growth speed and are isolated from soils of Ukraine
with different growth rate intensities. As a result we have shown the similarity of strains with an intense growth rate to soybean rhizobia of group USDA 123. The studied strains, as other representatives of this group, posssess increased saprophytic competence. The restriction analysis divided all sequences of the intergenic ITS region of soybean rhizobia into two ITS types: the first ITSStype includes strains with an intensive growth rate and the second ITS type contains strains with a slow grow rate. These types also coincide with their physioological groups.
Ecological genetics, 2013
We have conducted the analysis of sequences of the 16S rRNA gene and intergenic 16S 23S rRNA regi... more We have conducted the analysis of sequences of the 16S rRNA gene and intergenic 16S 23S rRNA region (ITS) of nodule bacteria of soybean which differ in growth speed and are isolated from soils of Ukraine with different growth rate intensities. As a result we have shown the similarity of strains with an intense growth rate to soybean rhizobia of group USDA 123. The studied strains, as other representatives of this group, pos sess increased saprophytic competence. The restriction analysis divided all sequences of the intergenic ITS region of soybean rhizobia into two ITS types: the first ITS type includes strains with an intensive growth rate and the second ITS type contains strains with a slow grow rate. These types also coincide with their physio logical groups.
Mikrobiolohichnyi Zhurnal
Agriciltural microbiology, 2016
In the vegetation experiment conditions it was established that the greatest effect of soybean ba... more In the vegetation experiment conditions it was established that the greatest effect of soybean bacterization can be obtained by two strains of nodule bacteria with slow (Bradyrhizobium japonicum 46) and intensive (B. japonicum КВ11) growth rates when these strains used in inoculum in the ratio 1 : 1 (binary composition). In compared to the mono-inoculation the combined use of these strains contributed the formation of balanced symbiotic system, increase the level of molecular nitrogen fixation, content of chlorophyll in the biomass leaves and above ground mass of different soybean plants varieties on 7.8–19.6 %. In a field experiment inoculation of soybean seeds with a composition of B. japonicum strains to increase the soybean yields by 11.1–13.7 % compared to the mono-inoculation.
Aim. To investigate the genetic diversity of soybean nodule bacteria, widespread in agrocenoses o... more Aim. To investigate the genetic diversity of soybean nodule bacteria, widespread in agrocenoses of Ukraine. Methods. Nodule bacteria were isolated from soybean nodules grown in soil samples selected in different regions of Ukraine. The amplification of the intergenic region 16S-23S rRNA (ITS-region) was conducted with the use of primers FGPS1490-72 and FGPL132-38. For PCR-products restriction there were applied restriction endonucleases MspI, HaeIII and NdeII. Results. The diversity of Bradyrhizobium japonicum strains with different growthrates was investigated using PCR-RFLP method. According to analysis of 16S-23S rDNA with enzymes MspI, HaeIII and NdeII it was obtained that all the intensivegrowing strains had the same restriction profiles, so they were combined into a single genetic group. Based on ITS-region restriction the slow-growing strains were assigned into two genetic groups using MspI enzyme, three genetic groups using HaeIII and four genomogroups using NdeII enzyme. Сo...
Проведён анализ последовательностей гена 16s рРнк и межгенного региона 16s-23s рРнк (ITS) клубен... more Проведён анализ последовательностей гена 16s рРнк и межгенного региона 16s-23s рРнк (ITS) клубеньковых
бактерий сои, отличающихся скоростью роста и выделенных
из почв Украины с различной интенсивностью выращивания
данной культуры. В результате показана близость штаммов
с интенсивным ростом к ризобиям сои группы USDA 123.
исследуемые штаммы, как и другие представители этой
группы, обладают повышенной сапрофитной компетентностью.
рестрикционный анализ последовательностей межгенного
региона ITS ризобий сои позволил разделить их на два ITS-типа:
первый ITS-тип — штаммы с интенсивным ростом и второй ITS-тип — медленнорастущие штаммы. такое разделение штаммов cоответствует распределению их на физиологические группы.
Із ґрунтів різних регіонів України вилучено 50 штамів бульбочкових бактерій квасолі та вивчено їх... more Із ґрунтів різних регіонів України вилучено 50 штамів бульбочкових бактерій квасолі та вивчено їх біологічне різноманіття. Встановлено, що нові штами Rhizobium phaseoli істотно відрізняються за чутливістю до антибіотиків, серологічними та генетичними властивостями. За антигенним складом досліджені бульбочкові бактерії віднесено до різних серологічних груп. Із застосуванням методу ПЛР-RFLP IТS-регіону виявлено значний генетичний поліморфізм мікросимбіонтів квасолі, виділених із ґрунтових популяцій ризобій. За рестрикційними профілями 16S-23S рДНК їх вперше розділено на різні IТS-типи.
Aim. To study genetic properties of soybean nodule bacteria with different growth rates, to resea... more Aim. To study genetic properties of soybean nodule bacteria with different growth rates, to research the ability of rhizobia to produce phytohormonal substances and estimate their symbiotic potential in the conditions of combined inoculation and mono-inoculation of soybean plants. Methods. Sequencing of the 16S-23S rDNA intergenic spacer of soybean rhizobia was performed on ABI 3130xl Genetic Analyzer. Determination of the qualitative and quantitative composition of phytohormones in culture liquid of Bradyrhizobium ja-ponicum strains was performed by SDTLC-chromatography of high resolution. Symbiotic potential of the strains was estimated in the field experiment in Polissia area of Ukraine. Results. It has been established that strains of soybean nodule bacteria with different growth rates by the structure of ITS region belong to different genetic groups: USDA 6, USDA 110 and USDA 123. The studied rhizobia differ in the levels of extracellular phy-tohormones synthesis: the slow-growing B. japonicum strain 46 produces more amounts of auxins, but the intensive-growing B. japonicum strain KB11 significantly exceeds in the amounts of cytokinins. It has been shown that an important factor of increasing soybean productivity is the formation of balanced symbiotic systems of the host plant with several complementary strains but serologically and genetically different strains of rhizobia of the same species. Conclusions. Combined inoculation of soybean seeds with two active strains B. japonicum 46 and B. japonicum KB11 allows more fully realizing the potential of symbiotic partners and receiving the higher yield. K e y w o r d s: Bradyrhizobium japonicum, soybean, 16S-23S rDNA (
In the vegetation experiment conditions it was established that the greatest effect of soybean ba... more In the vegetation experiment conditions it was established that the greatest effect of soybean bacterization can be obtained by two strains of nodule bacteria with slow (Bradyrhizobium japonicum 46) and intensive (B. japonicum КВ11) growth rates when these strains used in inocu-lum in the ratio 1 : 1 (binary composition). In compared to the mono-inoculation the combined use of these strains contributed the formation of balanced symbiotic system, increase the level of molecular nitrogen fixation, content of chlorophyll in the biomass leaves and above ground mass of different soybean plants varieties on 7.8–19.6 %. In a field experiment inoculation of soybean seeds with a composition of B. japonicum strains to increase the soybean yields by 11.1–13.7 % compared to the mono-inoculation.
We have conducted the analysis of sequences of the 16S rRNA gene and intergenic 16S-23S rRNA regi... more We have conducted the analysis of sequences of the 16S rRNA gene and intergenic 16S-23S rRNA region (ITS) of nodule bacteria of soybean which differ in growth speed and are isolated from soils of Ukraine
with different growth rate intensities. As a result we have shown the similarity of strains with an intense growth rate to soybean rhizobia of group USDA 123. The studied strains, as other representatives of this group, posssess increased saprophytic competence. The restriction analysis divided all sequences of the intergenic ITS region of soybean rhizobia into two ITS types: the first ITSStype includes strains with an intensive growth rate and the second ITS type contains strains with a slow grow rate. These types also coincide with their physioological groups.