A. Asti - Academia.edu (original) (raw)

Papers by A. Asti

Journal of Biomedical Materials Research Part A, 2010

The use of stem cells in regenerative medicine is an appealing area of research that has received... more The use of stem cells in regenerative medicine is an appealing area of research that has received a great deal of interest in recent years. The population called human adipose tissue-derived stem cells (hASCs) share many of the characteristic of its counterpart of marrow including extensive proliferative potential and the ability to undergo multilineage differentiation along classical mesenchymal lineages: adipogenesis, chondrogenesis, osteogenesis, and myogenesis. The aim of this study was to evaluate with biochemical and morphological methods the adhesion and differentiation of hASCs grown on trabecular titanium scaffolds. The hASCs isolated from subcutaneous adipose tissue after digestion with collagenase were seeded on monolayer and on trabecular titanium scaffolds and incubated at 37 C in 5% CO 2 with osteogenic medium or control medium.-The results showed that hASCs were able to adhere to titanium scaffolds, to proliferate, to acquire an osteoblastic-like phenotype, and to produce a calcified extracellular matrix with protein, such as, decorin, fibronectin, osteocalcin, osteonectin, osteopontin, and type I collagen. These data suggest that this kind of scaffold/cells construct is effective to regenerate damaged tissue and to restore the function of bone tissue. V

The adipose tissue has been taken during an hip prosthesis surgery. The hASCs, as staminal cells,... more The adipose tissue has been taken during an hip prosthesis surgery. The hASCs, as staminal cells, can differentiate in more cellular tipes. After been taken they are coltivated on plates till appear a cellular colture that express specific staminal markers. In this work the hASCs are seed on titanium trabecular scaffolds that allowed cellular adhesion and cellular growing. This complex should be used than to replace the lost bone tissue. To stimulate cells growing and differentiation it been added specific proliferative and osteogenic media that allowed to have an osteogenic population. To prove osteoblatic origin of the cells it been used specific laboratory methods that allowed to demonstrate the presence of specific bone proteins in the ECM, as alcalin fosfatasi, collagen I, osteopontin, osteocalcin and a calcific ECM that is deposited in the extracellular space by the cells.

PharmacoEconomics, 2018

As we reach the final issue of PharmacoEconomics for 2019, we wish to reflect on another successf... more As we reach the final issue of PharmacoEconomics for 2019, we wish to reflect on another successful year's achievements, and to thank all those who have contributed their time and effort to guarantee the high quality of our content. We thank the authors who have contributed articles to the journal over the course of 2019. The skill and dedication of all authors are critical to the continued publication of the journal. The quality of published articles is also testament to the significant efforts of the peer reviewers and our editorial board, whose commitment ensures that the journal's content is held to the highest possible standard. In addition to the editorial board, we would like to thank the following individuals who acted as reviewers for PharmacoEconomics in the last 12 months:

Atti del 20. …, 2006

... VIGO, DANIELE,; MUNARI, ELEONORA,; BENZONI, EMANUELA,; COLOMBANI, CARLA ANGIOLINA,; FAUSTINI,... more ... VIGO, DANIELE,; MUNARI, ELEONORA,; BENZONI, EMANUELA,; COLOMBANI, CARLA ANGIOLINA,; FAUSTINI, MASSIMO,; SESSO, LORENZO,; ML Torre, E. Munari, A. Asti, E. Benzoni, C. Colombani, M. Faustini, M. Michelis, L. Sesso, U. Conte, S. Villani, D. Vigo,; Associazione ...

Reproduction, Fertility and Development, 2002

A technology for encapsulation of swine semen in barium alginate and protamine alginate has recen... more A technology for encapsulation of swine semen in barium alginate and protamine alginate has recently been proposed for the controlled release of the spermatozoa, thus reducing the number of instrumental inseminations required. Controlled-release capsules containing swine spermatozoa were prepared by adding saturated BaCl2 solution to ejaculate and dropping the resulting suspension into a sodium alginate solution, leading to the formation of barium alginate capsules. A second type of capsule was obtained by cross-linking the barium alginate with protamine sulfate. Two types of membrane were thus obtained: barium alginate gel and a protamine cross-linked alginate membrane. Morphological (scanning electron microscopy and transmission electron microscopy), functional (motility, membrane integrity and in vitro fertilization test) and technological (capsule structure and weight) approaches were used to characterize the encapsulated spermatozoa and the controlled-delivery system. No differ...

... Citation: New three-dimensional culture system of porcine granulosa cells / D. Vigo, M. Faust... more ... Citation: New three-dimensional culture system of porcine granulosa cells / D. Vigo, M. Faustini, S. Villani, A. Asti, PA Accorsi, M. Spinaci, S. Scocca, A. Riccardi, G. Galeati, E. Munari, V. Russo, U. Conte, ML Torre - In: Prooceedings of the 18. ...

... ML Torre, M. Faustini, S. Villani, A. Asti, PA Accorsi, G. Galeati, E. Munari, V. Russo, U. C... more ... ML Torre, M. Faustini, S. Villani, A. Asti, PA Accorsi, G. Galeati, E. Munari, V. Russo, U. Conte, D. Vigo - In: International Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology / Organizzatori convegno. ...

... Citation: Intervertebral disc regeneration: morphological investigation of an in vitro recons... more ... Citation: Intervertebral disc regeneration: morphological investigation of an in vitro reconstructed tissue / A. Asti, A. Riccardi, E. Benzoni, M. Faustini, E. Munari, P. Gaetani, R. Rodriguez Y Baena, D. Levi, F. Pancioni, E. Aimar, ML Torre, D. Vigo - In: International Workshop on ...

Journal of medical microbiology, 2016

The purpose of this study is to describe the epidemiological surveillance of microbiological sent... more The purpose of this study is to describe the epidemiological surveillance of microbiological sentinel events (SEs) carried out between 2012 and 2014 at the Neurological Hospital Carlo Besta, Milano, Italy. The setting is inpatient care with multidrug-resistant infections. The aim of the procedure is to formalize the management mode, reporting and transmission of SEs. Categorical variables were described by counts and percentages, as mean and sd or median and interquartile range. The incidence rates of SE were calculated per 1000 patient-days and for 100 admissions using Poisson distribution. The incidence rate of isolation for 1000 patient-days varies from a minimum of 0.52 (95 % confidence interval, 0.23-1.15) for the second quarter of 2014 to a maximum value of 4.16 (95 % confidence interval, 3.20-5.40) for the first quarter of 2013. A decrease followed from the third quarter of 2013 that remained constant in 2014, reaching values similar to those of 2012. Preventive actions and t...

Journal of Hepatology, 1998

Viruses, 2021

The development and persistence of SARS-CoV-2-specific immune response in immunocompetent (IC) an... more The development and persistence of SARS-CoV-2-specific immune response in immunocompetent (IC) and immunocompromised patients is crucial for long-term protection. Immune response to SARS-CoV-2 infection was analysed in 57 IC and 15 solid organ transplanted (TX) patients. Antibody responses were determined by ELISA and neutralization assay. T-cell response was determined by stimulation with peptide pools of the Spike, Envelope, Membrane, and Nucleocapsid proteins with a 20-h Activation Induced Marker (AIM) and 7-day lymphoproliferative assays. Antibody response was detected at similar levels in IC and TX patients. Anti-Spike IgG, IgA and neutralizing antibodies persisted for at least one year, while anti-Nucleocapsid IgG declined earlier. Patients with pneumonia developed higher antibody levels than patients with mild symptoms. Similarly, both rapid and proliferative T-cell responses were detected within the first two months after infection at comparable levels in IC and TX patients,...

The International Journal of Artificial Organs, 2014

The formation of tissue produced by implanted cells is influenced greatly by the scaffold onto wh... more The formation of tissue produced by implanted cells is influenced greatly by the scaffold onto which they are seeded. In the long term it is often preferable to use a biodegradable material scaffold so that all the implanted materials will disappear, leaving behind only the generated tissue. Research in this area has identified several natural biodegradable materials. Among them, hydrogels are receiving increasing attention due to their ability to retain a great quantity of water, their good biocompatibility, their low interfacial tension, and the minimal mechanical and frictional irritation that they cause. Biocompatibility is not an intrinsic property of materials; rather it depends on the biological environment and the tolerability that exists with respect to specific polymer-tissue interactions. The most often utilized biodegradable synthetic polymers for 3D scaffolds in tissue engineering are saturated poly-a-hydroxy esters, including poly(lactic acid) (PLA) and poly(glycolic a...

Technology and Health Care, 2009

The objective of this study was to investigate the surface modification of a natural bone substit... more The objective of this study was to investigate the surface modification of a natural bone substitute, Bio-Oss, coated with a synthetic polymer poly-D,L-lactide (PLA), in order to improve cell growth. Bio-Oss is a natural bone substitute made of the mineralized portion of bovine bone. The material is used mainly to fill bone defects in periodontal and maxillofacial surgery and permit reossification. Poly-a-hydroxyacids such as polylactic acid are receiving an increasing attention due to their ability to retain a great quantity of water, good biocompatibility, low interfacial tension, and minimal mechanical and frictional irritation. All of these features are appealing from the perspective of bioenvironmental mimicking. The human osteosarcoma cell line SAOS-2 was added to the top of scaffolds uncoated or coated with PLA and incubated at 37 degrees in 5% CO(2) for 15 days. PLA-coated scaffolds improved cell growth. Polymer degradation behaviour, extraction and measurement of the extracellular matrix proteins of the cultured scaffolds (such as decorin, fibronectin osteocalcin, osteonectin, osteopontin and type-I and type-III collagen), immunolocalization of bone proteins and morphological analysis of the scaffolds confirmed the bioactive properties of Bio-Oss/PLA4M suggesting that it could be a valuable grafting material.

Rivista di parassitologia

Journal of Controlled Release, 2005

Prolidase is a cytosolic exopeptidase whose deficiency causes the development of a rare autosomal... more Prolidase is a cytosolic exopeptidase whose deficiency causes the development of a rare autosomal recessive disorder known as Prolidase Deficiency (PD). The main manifestations of PD are intractable ulcerations of the skin, recurrent infections and mental retardation. At this time only a hazardous and expensive chronic therapy based on blood transfusions is the suggested treatment for PD. The aim of this work was to investigate the capability of utilizing liposomes as enzyme carriers: these vesicular systems have been recently evaluated as protein carriers for their potential in terms of "in vivo" localization, drug release and for protein stabilization in biological fluids. Liposomes were prepared, with a 1:1 PC:Col molar ratio with or without DSPE-PEG, by a thin-film hydration. Ex-vivo experiments were performed, incubating prolidase loaded liposomes with cultured fibroblasts from PD patients and from controls, to determine the amount of active enzyme delivered to cells. Evaluation of liposomes toxicity on cultured skin fibroblasts showed that liposomes did not interfere with cellular growth. Results showed that all the active prolidase encapsulated in the liposomes was completely vehiculated inside fibroblasts after 6 days incubation. SEM analysis suggests that prolidase is vehiculated inside the cell through liposome endocytosis.

Digestive and Liver Disease, 2001

Bioinorganic Chemistry and Applications, 2010

Pluripotent adipose tissue-derived stem cells (hASCs) can differentiate into various mesodermal c... more Pluripotent adipose tissue-derived stem cells (hASCs) can differentiate into various mesodermal cell types such as osteoblasts, chondroblasts, and myoblasts. We isolated hASCs from subcutaneous adipose tissue during orthopaedic surgery and induced the osteogenic differentiation for 28 days on three different synthetic scaffolds such as polylactide-co-glycolide (PLGA), polylactide-co-glycolide/hydroxyapatite (PLGA/HA), and trabecular titanium scaffolds (Ti6Al4V). Pore size can influence certain criteria such as cell attachment, infiltration, and vascularization. The aim of this study was to investigate the performance of PLGA and PLGA/HA scaffolds with a higher porosity, ranging between 75% and 84%, with respect to Ti scaffolds but with smaller pore size, seeded with hASCs to develop a model that could be used in the treatment of bone defects and fractures. Osteogenesis was assessed by ELISA quantitation of extracellular matrix protein expression, von Kossa staining, X-ray microanaly...

Journal of Biomedical Materials Research Part A, 2010

The use of stem cells in regenerative medicine is an appealing area of research that has received... more The use of stem cells in regenerative medicine is an appealing area of research that has received a great deal of interest in recent years. The population called human adipose tissue-derived stem cells (hASCs) share many of the characteristic of its counterpart of marrow including extensive proliferative potential and the ability to undergo multilineage differentiation along classical mesenchymal lineages: adipogenesis, chondrogenesis, osteogenesis, and myogenesis. The aim of this study was to evaluate with biochemical and morphological methods the adhesion and differentiation of hASCs grown on trabecular titanium scaffolds. The hASCs isolated from subcutaneous adipose tissue after digestion with collagenase were seeded on monolayer and on trabecular titanium scaffolds and incubated at 37 C in 5% CO 2 with osteogenic medium or control medium.-The results showed that hASCs were able to adhere to titanium scaffolds, to proliferate, to acquire an osteoblastic-like phenotype, and to produce a calcified extracellular matrix with protein, such as, decorin, fibronectin, osteocalcin, osteonectin, osteopontin, and type I collagen. These data suggest that this kind of scaffold/cells construct is effective to regenerate damaged tissue and to restore the function of bone tissue. V

The adipose tissue has been taken during an hip prosthesis surgery. The hASCs, as staminal cells,... more The adipose tissue has been taken during an hip prosthesis surgery. The hASCs, as staminal cells, can differentiate in more cellular tipes. After been taken they are coltivated on plates till appear a cellular colture that express specific staminal markers. In this work the hASCs are seed on titanium trabecular scaffolds that allowed cellular adhesion and cellular growing. This complex should be used than to replace the lost bone tissue. To stimulate cells growing and differentiation it been added specific proliferative and osteogenic media that allowed to have an osteogenic population. To prove osteoblatic origin of the cells it been used specific laboratory methods that allowed to demonstrate the presence of specific bone proteins in the ECM, as alcalin fosfatasi, collagen I, osteopontin, osteocalcin and a calcific ECM that is deposited in the extracellular space by the cells.

PharmacoEconomics, 2018

As we reach the final issue of PharmacoEconomics for 2019, we wish to reflect on another successf... more As we reach the final issue of PharmacoEconomics for 2019, we wish to reflect on another successful year's achievements, and to thank all those who have contributed their time and effort to guarantee the high quality of our content. We thank the authors who have contributed articles to the journal over the course of 2019. The skill and dedication of all authors are critical to the continued publication of the journal. The quality of published articles is also testament to the significant efforts of the peer reviewers and our editorial board, whose commitment ensures that the journal's content is held to the highest possible standard. In addition to the editorial board, we would like to thank the following individuals who acted as reviewers for PharmacoEconomics in the last 12 months:

Atti del 20. …, 2006

... VIGO, DANIELE,; MUNARI, ELEONORA,; BENZONI, EMANUELA,; COLOMBANI, CARLA ANGIOLINA,; FAUSTINI,... more ... VIGO, DANIELE,; MUNARI, ELEONORA,; BENZONI, EMANUELA,; COLOMBANI, CARLA ANGIOLINA,; FAUSTINI, MASSIMO,; SESSO, LORENZO,; ML Torre, E. Munari, A. Asti, E. Benzoni, C. Colombani, M. Faustini, M. Michelis, L. Sesso, U. Conte, S. Villani, D. Vigo,; Associazione ...

Reproduction, Fertility and Development, 2002

A technology for encapsulation of swine semen in barium alginate and protamine alginate has recen... more A technology for encapsulation of swine semen in barium alginate and protamine alginate has recently been proposed for the controlled release of the spermatozoa, thus reducing the number of instrumental inseminations required. Controlled-release capsules containing swine spermatozoa were prepared by adding saturated BaCl2 solution to ejaculate and dropping the resulting suspension into a sodium alginate solution, leading to the formation of barium alginate capsules. A second type of capsule was obtained by cross-linking the barium alginate with protamine sulfate. Two types of membrane were thus obtained: barium alginate gel and a protamine cross-linked alginate membrane. Morphological (scanning electron microscopy and transmission electron microscopy), functional (motility, membrane integrity and in vitro fertilization test) and technological (capsule structure and weight) approaches were used to characterize the encapsulated spermatozoa and the controlled-delivery system. No differ...

... Citation: New three-dimensional culture system of porcine granulosa cells / D. Vigo, M. Faust... more ... Citation: New three-dimensional culture system of porcine granulosa cells / D. Vigo, M. Faustini, S. Villani, A. Asti, PA Accorsi, M. Spinaci, S. Scocca, A. Riccardi, G. Galeati, E. Munari, V. Russo, U. Conte, ML Torre - In: Prooceedings of the 18. ...

... ML Torre, M. Faustini, S. Villani, A. Asti, PA Accorsi, G. Galeati, E. Munari, V. Russo, U. C... more ... ML Torre, M. Faustini, S. Villani, A. Asti, PA Accorsi, G. Galeati, E. Munari, V. Russo, U. Conte, D. Vigo - In: International Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology / Organizzatori convegno. ...

... Citation: Intervertebral disc regeneration: morphological investigation of an in vitro recons... more ... Citation: Intervertebral disc regeneration: morphological investigation of an in vitro reconstructed tissue / A. Asti, A. Riccardi, E. Benzoni, M. Faustini, E. Munari, P. Gaetani, R. Rodriguez Y Baena, D. Levi, F. Pancioni, E. Aimar, ML Torre, D. Vigo - In: International Workshop on ...

Journal of medical microbiology, 2016

The purpose of this study is to describe the epidemiological surveillance of microbiological sent... more The purpose of this study is to describe the epidemiological surveillance of microbiological sentinel events (SEs) carried out between 2012 and 2014 at the Neurological Hospital Carlo Besta, Milano, Italy. The setting is inpatient care with multidrug-resistant infections. The aim of the procedure is to formalize the management mode, reporting and transmission of SEs. Categorical variables were described by counts and percentages, as mean and sd or median and interquartile range. The incidence rates of SE were calculated per 1000 patient-days and for 100 admissions using Poisson distribution. The incidence rate of isolation for 1000 patient-days varies from a minimum of 0.52 (95 % confidence interval, 0.23-1.15) for the second quarter of 2014 to a maximum value of 4.16 (95 % confidence interval, 3.20-5.40) for the first quarter of 2013. A decrease followed from the third quarter of 2013 that remained constant in 2014, reaching values similar to those of 2012. Preventive actions and t...

Journal of Hepatology, 1998

Viruses, 2021

The development and persistence of SARS-CoV-2-specific immune response in immunocompetent (IC) an... more The development and persistence of SARS-CoV-2-specific immune response in immunocompetent (IC) and immunocompromised patients is crucial for long-term protection. Immune response to SARS-CoV-2 infection was analysed in 57 IC and 15 solid organ transplanted (TX) patients. Antibody responses were determined by ELISA and neutralization assay. T-cell response was determined by stimulation with peptide pools of the Spike, Envelope, Membrane, and Nucleocapsid proteins with a 20-h Activation Induced Marker (AIM) and 7-day lymphoproliferative assays. Antibody response was detected at similar levels in IC and TX patients. Anti-Spike IgG, IgA and neutralizing antibodies persisted for at least one year, while anti-Nucleocapsid IgG declined earlier. Patients with pneumonia developed higher antibody levels than patients with mild symptoms. Similarly, both rapid and proliferative T-cell responses were detected within the first two months after infection at comparable levels in IC and TX patients,...

The International Journal of Artificial Organs, 2014

The formation of tissue produced by implanted cells is influenced greatly by the scaffold onto wh... more The formation of tissue produced by implanted cells is influenced greatly by the scaffold onto which they are seeded. In the long term it is often preferable to use a biodegradable material scaffold so that all the implanted materials will disappear, leaving behind only the generated tissue. Research in this area has identified several natural biodegradable materials. Among them, hydrogels are receiving increasing attention due to their ability to retain a great quantity of water, their good biocompatibility, their low interfacial tension, and the minimal mechanical and frictional irritation that they cause. Biocompatibility is not an intrinsic property of materials; rather it depends on the biological environment and the tolerability that exists with respect to specific polymer-tissue interactions. The most often utilized biodegradable synthetic polymers for 3D scaffolds in tissue engineering are saturated poly-a-hydroxy esters, including poly(lactic acid) (PLA) and poly(glycolic a...

Technology and Health Care, 2009

The objective of this study was to investigate the surface modification of a natural bone substit... more The objective of this study was to investigate the surface modification of a natural bone substitute, Bio-Oss, coated with a synthetic polymer poly-D,L-lactide (PLA), in order to improve cell growth. Bio-Oss is a natural bone substitute made of the mineralized portion of bovine bone. The material is used mainly to fill bone defects in periodontal and maxillofacial surgery and permit reossification. Poly-a-hydroxyacids such as polylactic acid are receiving an increasing attention due to their ability to retain a great quantity of water, good biocompatibility, low interfacial tension, and minimal mechanical and frictional irritation. All of these features are appealing from the perspective of bioenvironmental mimicking. The human osteosarcoma cell line SAOS-2 was added to the top of scaffolds uncoated or coated with PLA and incubated at 37 degrees in 5% CO(2) for 15 days. PLA-coated scaffolds improved cell growth. Polymer degradation behaviour, extraction and measurement of the extracellular matrix proteins of the cultured scaffolds (such as decorin, fibronectin osteocalcin, osteonectin, osteopontin and type-I and type-III collagen), immunolocalization of bone proteins and morphological analysis of the scaffolds confirmed the bioactive properties of Bio-Oss/PLA4M suggesting that it could be a valuable grafting material.

Rivista di parassitologia

Journal of Controlled Release, 2005

Prolidase is a cytosolic exopeptidase whose deficiency causes the development of a rare autosomal... more Prolidase is a cytosolic exopeptidase whose deficiency causes the development of a rare autosomal recessive disorder known as Prolidase Deficiency (PD). The main manifestations of PD are intractable ulcerations of the skin, recurrent infections and mental retardation. At this time only a hazardous and expensive chronic therapy based on blood transfusions is the suggested treatment for PD. The aim of this work was to investigate the capability of utilizing liposomes as enzyme carriers: these vesicular systems have been recently evaluated as protein carriers for their potential in terms of "in vivo" localization, drug release and for protein stabilization in biological fluids. Liposomes were prepared, with a 1:1 PC:Col molar ratio with or without DSPE-PEG, by a thin-film hydration. Ex-vivo experiments were performed, incubating prolidase loaded liposomes with cultured fibroblasts from PD patients and from controls, to determine the amount of active enzyme delivered to cells. Evaluation of liposomes toxicity on cultured skin fibroblasts showed that liposomes did not interfere with cellular growth. Results showed that all the active prolidase encapsulated in the liposomes was completely vehiculated inside fibroblasts after 6 days incubation. SEM analysis suggests that prolidase is vehiculated inside the cell through liposome endocytosis.

Digestive and Liver Disease, 2001

Bioinorganic Chemistry and Applications, 2010

Pluripotent adipose tissue-derived stem cells (hASCs) can differentiate into various mesodermal c... more Pluripotent adipose tissue-derived stem cells (hASCs) can differentiate into various mesodermal cell types such as osteoblasts, chondroblasts, and myoblasts. We isolated hASCs from subcutaneous adipose tissue during orthopaedic surgery and induced the osteogenic differentiation for 28 days on three different synthetic scaffolds such as polylactide-co-glycolide (PLGA), polylactide-co-glycolide/hydroxyapatite (PLGA/HA), and trabecular titanium scaffolds (Ti6Al4V). Pore size can influence certain criteria such as cell attachment, infiltration, and vascularization. The aim of this study was to investigate the performance of PLGA and PLGA/HA scaffolds with a higher porosity, ranging between 75% and 84%, with respect to Ti scaffolds but with smaller pore size, seeded with hASCs to develop a model that could be used in the treatment of bone defects and fractures. Osteogenesis was assessed by ELISA quantitation of extracellular matrix protein expression, von Kossa staining, X-ray microanaly...