ABDULMALIK AMINU - Academia.edu (original) (raw)
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Papers by ABDULMALIK AMINU
Analytical Biochemistry, 2022
We report a new assay system for the detection of miR-21 in cancer cells. The new assay works at ... more We report a new assay system for the detection of miR-21 in cancer cells. The new assay works at room temperature and it does not involve enzymatic amplification. It consists a hairpin smart probe, designed to specifically recognize miR-21 target sequence. We tested the performance and sequence recognition capability of the smart probe to confirm desired specifications. We used the smart probe for the sequence-specific recognition of synthetic miR-21 oligonucleotides as well as mismatch sequences and we found that the probe recognizes the target sequence-specifically, while discriminating against mismatched sequences. We determined the limit of detection and limit of quantitation for the miR-21 oligonucleotides to be 1.72 nM and 5.78 nM, respectively, while the sensitivity is 6.90 × 1011 c.p.sM-1. More importantly, we showed that the smart probe-based method is also sensitive and selective for miR-21 when applied to crude extractions from MCF-7 cancer cell line at room temperature, with the results showing high fluorescence signals for the MCF-7 samples while showing much less signals for samples that did not contain miR-21. Thus, this new smart probe system constitutes a homogeneous, mix-and-read detection technique that can provide reliable diagnostics of miR-21 cancer biomarker at room temperature.
Journal of Drug Delivery Science and Technology, 2020
The advancement in medical sciences and perturbing limitations of some conventional drug delivery... more The advancement in medical sciences and perturbing limitations of some conventional drug delivery systems have triggered extensive research in nanomedicine, and there have been profound exploitations that promise to revolutionise drug delivery systems. To develop more effective and safer therapeutic medicines, researchers have designed various advanced and multifunctional nanocarrier systems that can provide targeted, sustained, and controlled delivery of drugs. These novel systems are leading to the enhancement of drugs' systemic circulation, improvement of the pharmacokinetic profile of drugs, and reduction of adverse effect incidences of drugs. Some of the nanoparticulate drug delivery systems that have demonstrated substantial benefits such as polymeric, lipids and metals nanoparticles; liposomes; nanoemulsions; and micelles have already made it to the market. These nanocarriers have greatly influenced the pattern of modern drug therapy to address emerging needs. However, there are some concerns related to toxicological aspects associated with some of these nanocarriers that need to be resolved. This review article was aimed to provide insightful advancements on the applications of nanoparticulate drug delivery systems in the treatment of range of diseases in modern therapeutics. Particular emphasis was given to the roles of these nanocarriers in overcoming the prompted limitations of conventional drug delivery systems.
Analytical Biochemistry, 2022
We report a new assay system for the detection of miR-21 in cancer cells. The new assay works at ... more We report a new assay system for the detection of miR-21 in cancer cells. The new assay works at room temperature and it does not involve enzymatic amplification. It consists a hairpin smart probe, designed to specifically recognize miR-21 target sequence. We tested the performance and sequence recognition capability of the smart probe to confirm desired specifications. We used the smart probe for the sequence-specific recognition of synthetic miR-21 oligonucleotides as well as mismatch sequences and we found that the probe recognizes the target sequence-specifically, while discriminating against mismatched sequences. We determined the limit of detection and limit of quantitation for the miR-21 oligonucleotides to be 1.72 nM and 5.78 nM, respectively, while the sensitivity is 6.90 × 1011 c.p.sM-1. More importantly, we showed that the smart probe-based method is also sensitive and selective for miR-21 when applied to crude extractions from MCF-7 cancer cell line at room temperature, with the results showing high fluorescence signals for the MCF-7 samples while showing much less signals for samples that did not contain miR-21. Thus, this new smart probe system constitutes a homogeneous, mix-and-read detection technique that can provide reliable diagnostics of miR-21 cancer biomarker at room temperature.
Journal of Drug Delivery Science and Technology, 2020
The advancement in medical sciences and perturbing limitations of some conventional drug delivery... more The advancement in medical sciences and perturbing limitations of some conventional drug delivery systems have triggered extensive research in nanomedicine, and there have been profound exploitations that promise to revolutionise drug delivery systems. To develop more effective and safer therapeutic medicines, researchers have designed various advanced and multifunctional nanocarrier systems that can provide targeted, sustained, and controlled delivery of drugs. These novel systems are leading to the enhancement of drugs' systemic circulation, improvement of the pharmacokinetic profile of drugs, and reduction of adverse effect incidences of drugs. Some of the nanoparticulate drug delivery systems that have demonstrated substantial benefits such as polymeric, lipids and metals nanoparticles; liposomes; nanoemulsions; and micelles have already made it to the market. These nanocarriers have greatly influenced the pattern of modern drug therapy to address emerging needs. However, there are some concerns related to toxicological aspects associated with some of these nanocarriers that need to be resolved. This review article was aimed to provide insightful advancements on the applications of nanoparticulate drug delivery systems in the treatment of range of diseases in modern therapeutics. Particular emphasis was given to the roles of these nanocarriers in overcoming the prompted limitations of conventional drug delivery systems.