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Papers by Ali Benian

Journal of Bone Oncology, 2020

Background: Multiple myeloma (MM) is the second incurable hematological malignancy. In recent yea... more Background: Multiple myeloma (MM) is the second incurable hematological malignancy. In recent years, due to the rise of microRNA (miRNA), many scholars have participated in the study of its value in the diagnosis of MM, and have obtained good but inconsistent results. Therefore, in order to determine the role of miRNA in the early diagnosis of MM, we performed this meta-analysis. Methods: We searched for related studies including PubMed, Web of Science, EMBASE, Cochrane Library, China National Knowledge Infrastructure (CNKI) and Wanfang Database as of July 20, 2020 to conduct this metaanalysis. To improve the accuracy, the quality assessment of Diagnostic Accuracy Study 2 (QUADAS-2) was used. We also applied random effects models to summarize sensitivity and specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR) and area under the curve (AUC) to measure diagnostic values, and subgroup analysis used to discover potential sources of heterogeneity. Results: We finally collected 32 studies from 15 articles that included a total of 2053 MM patients and 1118 healthy controls in this meta-analysis. The overall sensitivity, specificity, PLR, NLR, DOR and AUC were 0.81, 0.85, 5.5, 0.22, 25 and 0.90, respectively. Subgroup analysis shows that the down-regulation of microRNA clusters with larger samples size of plasma type could carry out a better diagnostic accuracy of MM patients. In addition, publication bias was not found. Conclusions: Circulating miRNA could be a potential non-invasive biomarker for early diagnosis of MM. However, multi-center, more rigorous, and larger-scale studies are needed to verify our conclusions.

Turkiye Klinikleri Journal of Gynecology and Obstetrics, 2003

Gynecology & Obstetrics, 2015

Objective: Fetal intra-abdominal cystic masses are quite rare entities and their differential dia... more Objective: Fetal intra-abdominal cystic masses are quite rare entities and their differential diagnosis is particularly perplexing. These masses encompass many different pathological cysts originating from almost every organ in the abdomen. In female fetuses, ovarian cysts are the primary cause. In our study, we investigated the techniques used in diagnosis, accuracy of methods and management strategies, and tried to summarize postnatal outcomes. Materials and methods: A total of 29 cases were evaluated retrospectively by reviewing their ultrasonography (USG) results, magnetic resonance imaging (MRI) scans, interventions in perinatal period, postnatal follow up and surgical outcomes. Results: Twenty nine (25 female 4 male) cases were included in the study. Mean gestational week at diagnosis was 30,0 ± 6,4 for ovarian cysts and 24,7 ± 7,5 for non-ovarian cysts. Mean diameter of cysts was 41,7 ± 25,4 mm. 17 cysts (56%) were of ovarian origin, 6 (20,7%) were mesenteric cysts, 3 of them (10.3%) originated from kidneys and 3 (10.3%) of the cysts turned out to be choledochal-subhepatic cysts. In postnatal period, 8 cases required surgery which was ovarian and mesenteric cysts. In two incidences, gonads had to be removed. In differential diagnosis of masses, diagnostic accuracy of USG was calculated as 72,4% while that of MRI was 87.5%. Conclusion: Fetal abdominal cysts are seen more frequently in female fetuses and recognized relatively later during the pregnancy. Aspiration of the cysts in masses with larger diameters may be useful in reducing frequency of complications leading to gonad losses. Most common non-ovarian cysts are mesenteric cysts which also cause complications and require surgical interventions. Both USG and MRI are highly accurate imaging techniques in cases with adnexial masses. They have roughly the same accuracy in differential diagnosis of fetal abdominal cystic lesions. G y n e c o lo gy & O b s te tr ic s

Background and Design.-Analysis of umbilical cord blood acid-base status at birth could give some... more Background and Design.-Analysis of umbilical cord blood acid-base status at birth could give some information about the fetal intrapartal condition retrospectively. Normally umbilical cord arterial blood reflects fetal acid-base balance and umbilical cord venous blood reflects a combination of maternal acid-base status and placental function. In this study we aimed to demonstrate the relationship between the umbilical cord gas parameters and fetal well-being as well as to assess the usefulness of routine umbilical cord blood sampling. During a 12-month period we made the umbilical cord arterial and venous blood gas analysis of 199 healthy pregnant women between the 37th and 42nd week of gestation. During the trial all samples were taken by the same trained midewife and nurse. Immediately after each birth, the umbilical cord was clamped twice about 10 cm apart.Blood was taken first from the artery and from the vein using preheparinised syringes. Analysis was achieved within 30 minutes of delivery. Tracings indicative of fetal acidosis were defined as persistent late decelerations and severe variable decelerations (heart rate dropping to <70 beats/min and lasting >60 seconds). Results.-We classified the deliveries as normal (head or breech presentation with or without induction) and operative (Cesarean section, vacuum and forceps deliveries) to compare the fetal blood parameters. Each group was evaluated for the existence of intra-uterine growth retardation and fetal weight at delivery separately. Umbilical cord arterial pH, PO2 and venous pH, pCO2, pO2 difference between normal deliveries and operative deliveries done for fetal distress were statistically significant (p<0.05). Conclusion.-We conclude that umbilical cord blood pH and gases should be obtained only in cases of high risk pregnancies and in depressed newborns so that the diagnosis of neonatal asphyxia could be excluded in 80% of the cases.

Obstetrics & Gynecology, 2002

To investigate the plasma and placental levels of interleukin-10 (IL-10), transforming growth fac... more To investigate the plasma and placental levels of interleukin-10 (IL-10), transforming growth factor-␤ 1 (TGF-␤ 1), and epithelial-cadherin (E-cadherin) in normotensive and preeclamptic pregnancies. METHODS: The study population consisted of 33 women with normotensive pregnancy and 35 women with preeclampsia. Peripheral venous blood samples were collected before labor (35.3 ؎ 1.1 and 34.2 ؎ 3.4 weeks' gestation for normotensive and preeclamptic pregnancies, respectively), and placental tissues were obtained after delivery. Maternal plasma and placental homogenate IL-10, TGF-␤ 1 , and E-cadherin levels were determined by enzyme-linked immunosorbent assay. RESULTS: The mean plasma and placental levels of IL-10, TGF-␤ 1 , and E-cadherin were significantly higher in preeclamptic than normotensive patients (P < .001). The plasma and placental levels of IL-10, TGF-␤ 1 , and E-cadherin significantly increased with the increments in diastolic blood pressure (P < .001). CONCLUSION: IL-10, TGF-␤ 1 , and E-cadherin may be involved in the pathologic process of preeclampsia. The pathophysiologic changes associated with preeclampsia may stem in part from the overproduction of these placental mediators.

Gynecologic and Obstetric Investigation, 2005

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Arteriosclerosis, Thrombosis, and Vascular Biology, 2004

Objective— Oxidized low-density lipoprotein (Ox-LDL)is implicated in the pathogenesis of atherosc... more Objective— Oxidized low-density lipoprotein (Ox-LDL)is implicated in the pathogenesis of atherosclerosis. Circulating oxidation-specific epitopes on plasma Ox-LDL has been linked with coronary artery disease, but its determinants and its association with early development of atherosclerosis in familial combined hyperlipidemia (FCHL) has not been very well studied. This study aimed to investigate the determinants of the circulating Ox-LDL and the association between Ox-LDL and carotid intima-media thickness (IMT) in asymptomatic members of FCHL families. Methods and Results— Ox-LDL, susceptibility of LDL to oxidation in vitro, plasma 8-isoprostane and antioxidants, lipids and lipoproteins, LDL particle size, and carotid IMT were measured in 150 asymptomatic FCHL family members. Affected FCHL family members had reduced LDL particle size and lag time for LDL oxidation, increased plasma levels of Ox-LDL, increased plasma urate and α-tocopherol, and a trend for the increase of 8-isoprost...

Jinekoloji ve Obstetrik Derg., 2003

Plasma malondialdehyde, superoxide dismutase, sE-selectin, fibronectin, endothelin-1 and nitric o... more Plasma malondialdehyde, superoxide dismutase, sE-selectin, fibronectin, endothelin-1 and nitric oxide levels in women with preeclampsia

Ozet <;ali~mam1zda Klinigimizin vaginal anomali olgularmdaki yakla~1m1 irdelenerek uygulanan o... more Ozet <;ali~mam1zda Klinigimizin vaginal anomali olgularmdaki yakla~1m1 irdelenerek uygulanan operatif yfintemler ve sonuc;lan ortaya konulmaya c;ali~1ld1. Istanbul Oniversitesi Cerrahpa~ Tip Fakilltesi Kadm Hastallklan ve Dogum Kliniginde, 1974-2005 y1llan arasmda vaginal plastik ve rekonstruktif operasyon gec;iren olgularm dosyalan retrospektif olarak incelendi. Toplam 62 olgu c;ah§maya almch. Vaginal atrezi saptanan, Rokitansky- Kuster - Hauser Sendromu (RKH sendromu) ve vaginal darhg1 olan toplam 31 olguya Mcindoe operasyonu; transvers vaginal septum saptanan 16 olguya septum rezeksiyonu; himen imperforatus belirlenen 7 olguya himenotomi; vaginal ring saptanan 4 olguya ring eksizyonu; vagina ve serviks atrezisi olan 2 olguya trakelovestibiilostomi, Gartner kisti olan 2 olguya ise kist ekstirpasyonu uygulanm1§t1r. <;ogunlukla adolesan donemde belirti veren reprodiiktif geli§im anomalilerinin erken tams1 ve uygun tedavisi, reprodiiktif fonkslyonun korunmas1 ve bu olgularm psi...

International Journal of Gynecologic Cancer, 2004

Marmara Medical Journal, 2016

Objective: To estimate the relationship between maternal serum a- fetoprotein (MSAFP) levels and ... more Objective: To estimate the relationship between maternal serum a- fetoprotein (MSAFP) levels and uterine artery Doppler findings at 20th week of pregnancy for prediction of preeclampsia and intrauterine growth retardation (IUGR). Methods: Eighty pregnant women were followed as a study group prospectively. MSAFP levels at the 15-18th week of gestation and bilateral uterine artery Doppler findings at the 20th week of gestation were obtained. Results: Bilateral early diastolic notch of the uterine artery was detected in 17 cases (21,75%) at the 20th week of gestation. In 9 of them early diastolic notch was persistent at the 24th week while in 8 cases, it had disappeared. Uterine artery diastolic notch was found to be a significant predictive factor for preeclampsia, as well as for IUGR (p< 0.001, OR: 69.74; p<0.02, OR: 10.11, respectively). MSAFP levels of 17 cases that showed early diastolic notch in uterine artery Doppler were significantly higher than in the notch negative cas...

Placenta, 2021

INTRODUCTION In preeclampsia (PE), human decidua mesenchymal stromal cells (hDMSCs) are exposed t... more INTRODUCTION In preeclampsia (PE), human decidua mesenchymal stromal cells (hDMSCs) are exposed to abnormally high levels of oxidative stress and inflammatory factors circulating in the maternal blood. MicroRNAs (miRNAs) have been shown to have a significant impact on the differentiation, maturation and function of mesenchymal stromal cells (MSCs). Our aim in the present study is firstly to investigate differentially expressed miRNA levels to be used as a biomarker in the early detection of PE and secondly to investigate whether those differentially expressed miRNAs in hDMSCs have an effect on the pathogenesis of PE. METHODS This study covers miRNA expression analysis of hDMSCs from 7 PE patient and 7 healthy pregnant women and is a preliminary study to investigate putative biomarkers. After cell culture and cell sorting, total RNA including miRNAs were isolated from hDMSCs. Let-7b-3p, let-7f-1-3p, miR-191-3p, miR-550a-5p, miR-33b-3p and miR-425-3p were used for miRNA analysis and U6 snRNA was used for normalization of the samples. MiRNA analysis was performed by droplet digital polymerase chain reaction (ddPCR) method and obtained results were evaluated statistically. RESULTS As a result of the analysis, it was observed that the levels of hsa-miR-33b-3p significantly (AUC: 0.93, p = 0.04, fold change: 4.5) increased in hDMSC of PE patients compared to healthy controls. However, let-7b-3p, let-7f-1-3p, miR-191-3p, miR-550a-5p, and miR-425-3p were not considered as significant because they did not meet the p < 0,05 requirement. DISCUSSION Within the scope of the study, it is predicted that miR-33b-3p (p = 0.004, AUC = 0.93) can be used as a biomarker in detecting PE.

International Journal of Gynecologic Cancer, 2003

Canadian journal of diabetes, Jan 2, 2018

Intercellular adhesion molecule-1 (ICAM-1) plays an important role in endothelial function. Hyper... more Intercellular adhesion molecule-1 (ICAM-1) plays an important role in endothelial function. Hyperglycemia-induced impaired redox status is 1 of the well-known pathophysiologic characteristics of gestational diabetes mellitus (GDM), and it plays a crucial role in the causes of disease. Our aim was to clarify any possible relationship between the ICAM-1 Lys469Glu polymorphism and systemic redox status in women with and without GDM. Also, we investigated whether this polymorphism could be associated with a change for better or worse as evidenced by clinical and redox biomarkers. The ICAM-1 polymorphism statuses of 89 pregnant women without GDM and 53 pregnant women with GDM were found. Stratifying patients based on GDM and polymorphism status, we investigated various redox homeostasis markers. The independent t test was used. Significantly higher systemic oxidative damage and diminished antioxidant defense were found in pregnant women with GDM. Also, results showed that whether pregnan...

Molecular medicine reports, 2018

Early pregnancy loss (EPL), also termed early miscarriage, is determined as the unintentional exp... more Early pregnancy loss (EPL), also termed early miscarriage, is determined as the unintentional expulsion of an embryo or fetus prior to the 12th week of gestation. EPL frequency is ~15% in pregnancies. Fetal development and growth is associate with placental function and vessel development; therefore, the placental genome would represent a useful miscarriage model for (epi)genetic and genomic studies. An important factor of placental development and function is epigenetic regulation of gene expression. microRNAs (miRNAs) are the primary epigenetic regulators which have an important role in placental development and function. In the present study, maternal plasma and villous tissue were collected from 16 EPL cases in 6th‑8th gestational weeks (GWs) and 8 abortions (control group) in 6th‑8th GWs. Detection of the differences in miRNA expression was performed using microarrays and dysregulated miRNAs were validated by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR...

The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians, Jan 5, 2018

Preeclampsia (PE) is a pregnancy specific disease soon after 20 weeks of gestation where major sy... more Preeclampsia (PE) is a pregnancy specific disease soon after 20 weeks of gestation where major symptoms are hypertension and proteinuria. The underlying pathology is believed to be abnormal placentation. Epigenetic and genetic factors have significant roles in abnormal placental development. MicroRNA's (miRNAs), being one of the most important epigenetic regulators, take part in abnormal placentation. Hsa-miR-195 is a molecule associated with abnormal placental growth mechanisms such as impaired cellular proliferation, inadequate trophoblastic invasion causing defective spiral artery remodeling and apoptosis. We aimed to evaluate miRNA functions, namely miR-195 expression profile, in order to divulge preeclampsia pathogenesis. In this study, we extracted circulating miRNAs from maternal plasma and placenta from 20 PE patients and 20 normotensive pregnant women. MiR-195 was quantified using quantitative real time reverse transcriptase PCR (qRT-PCR). The target genes of miR-195 we...

Placenta, 2017

Preeclampsia (PE) is one of the leading causes of maternal and fetal morbidity and mortality, occ... more Preeclampsia (PE) is one of the leading causes of maternal and fetal morbidity and mortality, occurring usually in the second half of pregnancy and affecting approximately 5-8% of pregnancies in the world. miRNAs play critical role in the regulation of placental development processes. We aimed to determine specific novel miRNAs for early diagnosis of preeclampsia which is one of the most dangerous pregnancy diseases. In this study 72 samples, maternal age 22≤ and ≤36, have been analyzed; maternal plasma and placental miRNAs were isolated from 18 severe preeclampsia (sPE) patients and 18 controls, respectively. Profiling of human miRNAs (1368 probe) was performed in samples with Agilent v16 microarrays for detection of the differences in miRNA expression between two groups. The results were validated by using TaqMan RT-qPCR method. The analysis indicated that 406 of these miRNAs in all placentas and 42 of these miRNAs in all maternal plasma were expressed. The relative expression analysis has shown that 12 miRNAs (p < 0.05 and >2-fold) in maternal plasma were differentially expressed in PE and control group. However, five miRNAs were validated by qRT-PCR. Once validated miRNAs have been searched in databases for their target genes and function, it has been shown that there are some preeclampsia related pathways as a target such as angiogenesis, cardiovascular, hypertension, placental abruption and preeclampsia disorders. Differentially expressed and validated plasma miRNAs might be used as notable biomarkers for non-invasive early diagnosis of preeclampsia and treatment of disease.

Bangladesh Journal of Obstetrics & Gynaecology, 2016

The ratios of maternal and perinatal mortality are indicators of the health status of a nation.Ma... more The ratios of maternal and perinatal mortality are indicators of the health status of a nation.Maternal near-miss ratio is more commonly used than maternal mortality ratio because it is more frequent than maternal deaths, making for more detailed analyses of the problems in obstetric care 1. High maternal near miss/ maternal mortality ratio and low mortality index show good quality of obstetric care 2 .