A. Bryanskaya - Academia.edu (original) (raw)

Papers by A. Bryanskaya

Additional file 2: Table S1. Water samples. Note: b.d.l. – below detected limit, n.d. – no data.

The 5-th lnternational Conference "Terahertz and Microwave Radiation: Generation, Detection and Applications" (ТЕRА-2023). Abstract book

International Journal of Astrobiology, 2019

Mars is considered to be one of the most favourable places in the Solar System to search for past... more Mars is considered to be one of the most favourable places in the Solar System to search for past and present life. In the past Mars was warmer and wetter, so terrestrial halophiles can be regarded as analogues of hypothetical ancient Martian halophiles. In this study we used microorganisms from unique Altai region (Russia) to estimate the capability of terrestrial bacteria and archaea to survive at low temperatures and high concentration of salts and metals, similar to the Martian environment. The current report demonstrates that both halophilic archaea and halotolerant bacteria from saline lakes of the Altai region may be considered as analogues of ancient Martian organisms, since they are able to withstand conditions that hypothetically existed in subsurface layers of the early Mars (low temperatures, salt solutions with a high content of NaCl) with only slight decrease in viability. We also found that the studied microorganisms can use some organic substances found in meteorites...

Geochimica et Cosmochimica Acta Supplement, Jun 1, 2009

Geochimica et Cosmochimica Acta Supplement, Jul 1, 2008

Genome announcements, Jan 13, 2017

Undisturbed hot springs inside Yellowstone National Park remain a dynamic biome for novel cellulo... more Undisturbed hot springs inside Yellowstone National Park remain a dynamic biome for novel cellulolytic thermophiles. We report here the draft genome sequence of one of these isolates, Bacillus altitudinis YNP4-TSU.

Standards in Genomic Sciences, 2016

The members of the genus Thermoactinomyces are known for their protein degradative capacities. Th... more The members of the genus Thermoactinomyces are known for their protein degradative capacities. Thermoactinomyces sp. strain AS95 is a Gram-positive filamentous bacterium, isolated from moderately saline water in the Thamelaht region of Algeria. This isolate is a thermophilic aerobic bacterium with the capacity to produce extracellular proteolytic enzymes. This strain exhibits up to 99 % similarity with members of the genus Thermoactinomyces, based on 16S rRNA gene sequence similarity. Here we report on the phenotypic features of Thermoactinomyces sp. strain AS95 together with the draft genome sequence and its annotation. The genome of this strain is 2,558,690 bp in length (one chromosome, but no plasmid) with an average G + C content of 47.95 %, and contains 2550 protein-coding and 60 RNA genes together with 64 ORFs annotated as proteases.

GCB Bioenergy, 2016

In this study, we used ELISA for quantification of PPDK in photosynthesizing leaves of miscanthus... more In this study, we used ELISA for quantification of PPDK in photosynthesizing leaves of miscanthus. We cloned a fragment of the gene encoding PPDK, purified the resulting protein by affinity chromatography, identified it using MALDI mass spectrometry, and obtained monoclonal antibodies by immunizing BALB/c mice. Selectivity of monoclonal antibodies was assessed by Western blot using the protein extracts of Soranovskii. The presence of PPDK was again verified by MALDI mass spectrometry. Therefore, we developed and tested the method for determining PPDK quantity in miscanthus using ELISA.

Microbiology, 2018

⎯A comprehensive research of water and cyanobacterial mats in Mogoysky and Shurindinsky thermal s... more ⎯A comprehensive research of water and cyanobacterial mats in Mogoysky and Shurindinsky thermal springs (Baikal rift zone) was carried out by hydrochemical, chemical, microbiological, and mineralogical methods. Detailed descriptions of the springs location and their characteristics were given. According to their chemical composition, the springs were classified as fluoride-bicarbonate (Mogoysky) and bicarbonatesulfate (Shurindinsky) types with a high concentration of fluorine. This is explained by the interaction of infiltration waters with embedding rocks. A wide diversity of cyanobacteria (14 species of 7 genera) was revealed in the investigated springs. The development of cyanobacteria in microbial mats was observed at water outflows at the temperatures of 37.8 to 76.6°C. Chlorophyll a was the predominant pigment in microbial mats of the studied springs, indicating predominance of cyanobacteria in the mat. Deposition of various pyrite forms, celestite (SrSO 4), fluorite (CaF 2), calcium carbonate, elemental sulfur, barite, and amorphous silica was found in microbial mats.

Vavilovskij Žurnal Genetiki i Selekcii, 2014

The prospects of new alga strains as a source of non-food renewable biomass suitable for third ge... more The prospects of new alga strains as a source of non-food renewable biomass suitable for third generation biofuel production are discussed. West Siberia is of special interest, as it has a broad range of habitats and weakly studied microalga biodiversity. During this study, a number of strains were isolated. Of them, Chlorella spp. A1125 met the requirements for cultivation in a pilot-scale photobioreactor: high biomass productivity and high lipid content (0,081 g/l, or 23 % dry weight). The strain had high contents of saturated C16:0 (25 %) and unsaturated fatty acids: C16:2 (16 %) and C18:2 (27 %), thus being promising for catalytic production of third generation biofuel.

Vavilovskij Žurnal Genetiki i Selekcii, 2015

Rapid and accurate methods of microorganism identification are essential in various human activit... more Rapid and accurate methods of microorganism identification are essential in various human activities. They include primarily clinical diagnostics. In addition, they are in demand in environment monitoring, harmacology, food industry, research, etc. There are diverse approaches to microbe identification: phenotyping, genotyping, chemotaxonomy, direct protein profiling, etc. In this work, the effects of various growth conditions, such as temperature, growth time, and nutrition medium, on the reproducibility of microbe identification by MALDI-TOF mass spectrometry are considered by the example of three Geobacillus stearothermоphilus strains.

1. Разумовский С. М. Закономерности динамики биогеоценозов. М.: Наука, 1981. 231 с. 2. Лихошвай В... more 1. Разумовский С. М. Закономерности динамики биогеоценозов. М.: Наука, 1981. 231 с. 2. Лихошвай В. А., Ратушный А. В., Бажан С. И. и др. Методы моделирования динамики молекулярно-генетических систем // Системная Компьютерная биология / Под ред. Н. А. Колчанова, С. С. Гончарова, В. А. Лихошвай, В. А. Иванисенко. Новосибирск, 2008. C. 333–396. 3. Тимонов В. С., Мигинский Д. С., Колчанов Н. А. Технология реконструкции и анализа сетевых моделей экологических систем // Молодежь и современные информационные технологии: Сб. тр. VII Всерос. науч.-практ. конф. студентов, аспирантов и молодых ученых. Томск: Изд-во «СПБ Графикс», 2009. Т. 1. С. 335–336. 4. Сергеев М. Г., Суслов В. В., Мигинский Д. С. и др. Опыт создания базы данных для описания экосистем с использованием сетевых технологий // Биоразнообразие и динамика экосистем / Под ред. В. К. Шумного, Ю. И. Шокина, Н. А. Колчанова, А. М. Федотова. Новосибирск, 2006. C. 95–116. 5. Timonov V. S., Gunbin K. V., Turnaev I. I., Genaev M. A., Mig...

Microbiology Resource Announcements, 2019

Halorubrum sp. strain 48-1-W was isolated from a water sample from a saline lake (Novosibirsk Reg... more Halorubrum sp. strain 48-1-W was isolated from a water sample from a saline lake (Novosibirsk Region, Russia, 54°14′N 78°13′E). The sequenced and annotated genome is 3,584,929 bp and contains 3,506 genes.

Perennial Biomass Crops for a Resource-Constrained World, 2016

We developed a novel miscanthus cultivar, Soranovskii, based on Far Eastern populations that were... more We developed a novel miscanthus cultivar, Soranovskii, based on Far Eastern populations that were successfully cultivated in West Siberia. Its plastid DNA belongs to M. sacchariflorus. We analyzed the saccharification of Soranovskii biomass compared with other species: Phalaris arundinacea, Thrachomitum lancifolium, and Sida hermaphrodita. The biomass of Phalaris was the most easily hydrolyzed. Milling and pretreatment by alkaline peroxide provided optimal conditions for complete enzymatic hydrolysis of miscanthus biomass. As the result, we obtained a sugar-containing substrate suitable for subsequent fermentation by microorganisms to ethanol and other substances. We also attempted to isolate cellulosolytic microorganisms from natural communities and to assess their biotechnological potential. Among the studied microorganisms we detected potential producers of ethanol and lactic acid on miscanthus.

Microbiology Resource Announcements, 2019

The Halomonas sp. isolate SL48-SHIP-3 genome was obtained from metagenomics sequencing of the mic... more The Halomonas sp. isolate SL48-SHIP-3 genome was obtained from metagenomics sequencing of the microbial mat of Salt Lake Number 48 (54.201806N, 78.179194E; Novosibirsk region, Russia). The sequenced and annotated genome is 2,575,909 bp and encodes 2,368 genes.

Microbiology, 2006

The structure and production characteristics of microbial communities from the Urinskii alkaline ... more The structure and production characteristics of microbial communities from the Urinskii alkaline hot spring (Buryat Republic, Russia) have been investigated. A distinctive characteristic of this hot spring is the lack of sulfide in the issuing water. The water temperature near the spring vents ranged from 69 to 38.5 degrees C and pH values ranged from 8.8 to 9.2. The total mineralization of water was less than 0.1 g/liter. Temperature has a profound effect on the species composition and biogeochemical processes occurring in the algal-bacterial mats of the Urinskii hot spring. The maximum diversity of the phototrophic community was observed at the temperatures 40 and 46 degrees C. A total of 12 species of cyanobacteria, 4 species of diatoms, and one species of thermophilic anoxygenic phototrophic bacteria, Chloroflexus aurantiacus, have been isolated from mat samples. At temperatures above 40 degrees C, the filamentous cyanobacterium Phormidium laminosum was predominant; its cell number and biomass concentration were 95.1 and 63.9%, respectively. At lower temperatures, the biomass concentrations of the cyanobacterium Oscillatoria limosa and diatoms increased (50.2 and 36.4%, respectively). The cyanobacterium Mastigocladus laminosus, which is normally found in neutral or slightly acidic hydrothermal systems, was detected in microbial communities. As the diatom concentration increases, so does the dry matter concentration in mats, while the content of organic matter decreases. The concentrations of proteins and carbohydrates reached their maximum levels at 45-50 degrees C. The maximum average rate of oxygenic photosynthesis (2.1 g C/m2 day), chlorophyll a content (343.4 mg/m2), and cell number of phototrophic microorganisms were observed at temperatures from 45 to 50 degrees C. The peak mass of bacterial mats (56.75 g/m2) occurred at a temperature of 65-60 degrees C. The maximum biomass concentration of phototrophs (414.63 x 10(-6) g/ml) and the peak rate of anoxygenic photosynthesis [0.42 g C/(m2 day)] were observed at a temperature of 35-40 degrees C.

This article appeared in a journal published by Elsevier. The attached copy is furnished to the a... more This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues. Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. In most cases authors are permitted to post their version of the article (e.g. in Word or Tex form) to their personal website or institutional repository. Authors requiring further information regarding Elsevier's archiving and manuscript policies are encouraged to visit: http://www.elsevier.com/copyright

Doklady Earth Sciences

Data indicating the important role of microorganisms in the redistribution of REEs in the weather... more Data indicating the important role of microorganisms in the redistribution of REEs in the weathering crust and the decisive role in the concentration of REEs during the formation of ores in the upper ore horizon of the Tomtor field are obtained. The uptake of REEs was carried out by the community of microorganisms, such as phototrophs, methanogens, methanotrophs, and proteobacteria, which form the basis of the microbiocenosis for this paleoecosystem. The isotopic composition of C carbonates in all samples studied with fossilized microorganisms corresponds to the biogenic one, and the isotopic composition δ18ОSMOW (from 7 to 20‰) indicates the endogenous (hydrothermal) and, to a lesser extent, exogenous nature of the solutions. The low (87Sr/86Sr)I values of carbonates (~0.7036–0.7042) exclude the participation of seawater.

Additional file 2: Table S1. Water samples. Note: b.d.l. – below detected limit, n.d. – no data.

The 5-th lnternational Conference "Terahertz and Microwave Radiation: Generation, Detection and Applications" (ТЕRА-2023). Abstract book

International Journal of Astrobiology, 2019

Mars is considered to be one of the most favourable places in the Solar System to search for past... more Mars is considered to be one of the most favourable places in the Solar System to search for past and present life. In the past Mars was warmer and wetter, so terrestrial halophiles can be regarded as analogues of hypothetical ancient Martian halophiles. In this study we used microorganisms from unique Altai region (Russia) to estimate the capability of terrestrial bacteria and archaea to survive at low temperatures and high concentration of salts and metals, similar to the Martian environment. The current report demonstrates that both halophilic archaea and halotolerant bacteria from saline lakes of the Altai region may be considered as analogues of ancient Martian organisms, since they are able to withstand conditions that hypothetically existed in subsurface layers of the early Mars (low temperatures, salt solutions with a high content of NaCl) with only slight decrease in viability. We also found that the studied microorganisms can use some organic substances found in meteorites...

Geochimica et Cosmochimica Acta Supplement, Jun 1, 2009

Geochimica et Cosmochimica Acta Supplement, Jul 1, 2008

Genome announcements, Jan 13, 2017

Undisturbed hot springs inside Yellowstone National Park remain a dynamic biome for novel cellulo... more Undisturbed hot springs inside Yellowstone National Park remain a dynamic biome for novel cellulolytic thermophiles. We report here the draft genome sequence of one of these isolates, Bacillus altitudinis YNP4-TSU.

Standards in Genomic Sciences, 2016

The members of the genus Thermoactinomyces are known for their protein degradative capacities. Th... more The members of the genus Thermoactinomyces are known for their protein degradative capacities. Thermoactinomyces sp. strain AS95 is a Gram-positive filamentous bacterium, isolated from moderately saline water in the Thamelaht region of Algeria. This isolate is a thermophilic aerobic bacterium with the capacity to produce extracellular proteolytic enzymes. This strain exhibits up to 99 % similarity with members of the genus Thermoactinomyces, based on 16S rRNA gene sequence similarity. Here we report on the phenotypic features of Thermoactinomyces sp. strain AS95 together with the draft genome sequence and its annotation. The genome of this strain is 2,558,690 bp in length (one chromosome, but no plasmid) with an average G + C content of 47.95 %, and contains 2550 protein-coding and 60 RNA genes together with 64 ORFs annotated as proteases.

GCB Bioenergy, 2016

In this study, we used ELISA for quantification of PPDK in photosynthesizing leaves of miscanthus... more In this study, we used ELISA for quantification of PPDK in photosynthesizing leaves of miscanthus. We cloned a fragment of the gene encoding PPDK, purified the resulting protein by affinity chromatography, identified it using MALDI mass spectrometry, and obtained monoclonal antibodies by immunizing BALB/c mice. Selectivity of monoclonal antibodies was assessed by Western blot using the protein extracts of Soranovskii. The presence of PPDK was again verified by MALDI mass spectrometry. Therefore, we developed and tested the method for determining PPDK quantity in miscanthus using ELISA.

Microbiology, 2018

⎯A comprehensive research of water and cyanobacterial mats in Mogoysky and Shurindinsky thermal s... more ⎯A comprehensive research of water and cyanobacterial mats in Mogoysky and Shurindinsky thermal springs (Baikal rift zone) was carried out by hydrochemical, chemical, microbiological, and mineralogical methods. Detailed descriptions of the springs location and their characteristics were given. According to their chemical composition, the springs were classified as fluoride-bicarbonate (Mogoysky) and bicarbonatesulfate (Shurindinsky) types with a high concentration of fluorine. This is explained by the interaction of infiltration waters with embedding rocks. A wide diversity of cyanobacteria (14 species of 7 genera) was revealed in the investigated springs. The development of cyanobacteria in microbial mats was observed at water outflows at the temperatures of 37.8 to 76.6°C. Chlorophyll a was the predominant pigment in microbial mats of the studied springs, indicating predominance of cyanobacteria in the mat. Deposition of various pyrite forms, celestite (SrSO 4), fluorite (CaF 2), calcium carbonate, elemental sulfur, barite, and amorphous silica was found in microbial mats.

Vavilovskij Žurnal Genetiki i Selekcii, 2014

The prospects of new alga strains as a source of non-food renewable biomass suitable for third ge... more The prospects of new alga strains as a source of non-food renewable biomass suitable for third generation biofuel production are discussed. West Siberia is of special interest, as it has a broad range of habitats and weakly studied microalga biodiversity. During this study, a number of strains were isolated. Of them, Chlorella spp. A1125 met the requirements for cultivation in a pilot-scale photobioreactor: high biomass productivity and high lipid content (0,081 g/l, or 23 % dry weight). The strain had high contents of saturated C16:0 (25 %) and unsaturated fatty acids: C16:2 (16 %) and C18:2 (27 %), thus being promising for catalytic production of third generation biofuel.

Vavilovskij Žurnal Genetiki i Selekcii, 2015

Rapid and accurate methods of microorganism identification are essential in various human activit... more Rapid and accurate methods of microorganism identification are essential in various human activities. They include primarily clinical diagnostics. In addition, they are in demand in environment monitoring, harmacology, food industry, research, etc. There are diverse approaches to microbe identification: phenotyping, genotyping, chemotaxonomy, direct protein profiling, etc. In this work, the effects of various growth conditions, such as temperature, growth time, and nutrition medium, on the reproducibility of microbe identification by MALDI-TOF mass spectrometry are considered by the example of three Geobacillus stearothermоphilus strains.

1. Разумовский С. М. Закономерности динамики биогеоценозов. М.: Наука, 1981. 231 с. 2. Лихошвай В... more 1. Разумовский С. М. Закономерности динамики биогеоценозов. М.: Наука, 1981. 231 с. 2. Лихошвай В. А., Ратушный А. В., Бажан С. И. и др. Методы моделирования динамики молекулярно-генетических систем // Системная Компьютерная биология / Под ред. Н. А. Колчанова, С. С. Гончарова, В. А. Лихошвай, В. А. Иванисенко. Новосибирск, 2008. C. 333–396. 3. Тимонов В. С., Мигинский Д. С., Колчанов Н. А. Технология реконструкции и анализа сетевых моделей экологических систем // Молодежь и современные информационные технологии: Сб. тр. VII Всерос. науч.-практ. конф. студентов, аспирантов и молодых ученых. Томск: Изд-во «СПБ Графикс», 2009. Т. 1. С. 335–336. 4. Сергеев М. Г., Суслов В. В., Мигинский Д. С. и др. Опыт создания базы данных для описания экосистем с использованием сетевых технологий // Биоразнообразие и динамика экосистем / Под ред. В. К. Шумного, Ю. И. Шокина, Н. А. Колчанова, А. М. Федотова. Новосибирск, 2006. C. 95–116. 5. Timonov V. S., Gunbin K. V., Turnaev I. I., Genaev M. A., Mig...

Microbiology Resource Announcements, 2019

Halorubrum sp. strain 48-1-W was isolated from a water sample from a saline lake (Novosibirsk Reg... more Halorubrum sp. strain 48-1-W was isolated from a water sample from a saline lake (Novosibirsk Region, Russia, 54°14′N 78°13′E). The sequenced and annotated genome is 3,584,929 bp and contains 3,506 genes.

Perennial Biomass Crops for a Resource-Constrained World, 2016

We developed a novel miscanthus cultivar, Soranovskii, based on Far Eastern populations that were... more We developed a novel miscanthus cultivar, Soranovskii, based on Far Eastern populations that were successfully cultivated in West Siberia. Its plastid DNA belongs to M. sacchariflorus. We analyzed the saccharification of Soranovskii biomass compared with other species: Phalaris arundinacea, Thrachomitum lancifolium, and Sida hermaphrodita. The biomass of Phalaris was the most easily hydrolyzed. Milling and pretreatment by alkaline peroxide provided optimal conditions for complete enzymatic hydrolysis of miscanthus biomass. As the result, we obtained a sugar-containing substrate suitable for subsequent fermentation by microorganisms to ethanol and other substances. We also attempted to isolate cellulosolytic microorganisms from natural communities and to assess their biotechnological potential. Among the studied microorganisms we detected potential producers of ethanol and lactic acid on miscanthus.

Microbiology Resource Announcements, 2019

The Halomonas sp. isolate SL48-SHIP-3 genome was obtained from metagenomics sequencing of the mic... more The Halomonas sp. isolate SL48-SHIP-3 genome was obtained from metagenomics sequencing of the microbial mat of Salt Lake Number 48 (54.201806N, 78.179194E; Novosibirsk region, Russia). The sequenced and annotated genome is 2,575,909 bp and encodes 2,368 genes.

Microbiology, 2006

The structure and production characteristics of microbial communities from the Urinskii alkaline ... more The structure and production characteristics of microbial communities from the Urinskii alkaline hot spring (Buryat Republic, Russia) have been investigated. A distinctive characteristic of this hot spring is the lack of sulfide in the issuing water. The water temperature near the spring vents ranged from 69 to 38.5 degrees C and pH values ranged from 8.8 to 9.2. The total mineralization of water was less than 0.1 g/liter. Temperature has a profound effect on the species composition and biogeochemical processes occurring in the algal-bacterial mats of the Urinskii hot spring. The maximum diversity of the phototrophic community was observed at the temperatures 40 and 46 degrees C. A total of 12 species of cyanobacteria, 4 species of diatoms, and one species of thermophilic anoxygenic phototrophic bacteria, Chloroflexus aurantiacus, have been isolated from mat samples. At temperatures above 40 degrees C, the filamentous cyanobacterium Phormidium laminosum was predominant; its cell number and biomass concentration were 95.1 and 63.9%, respectively. At lower temperatures, the biomass concentrations of the cyanobacterium Oscillatoria limosa and diatoms increased (50.2 and 36.4%, respectively). The cyanobacterium Mastigocladus laminosus, which is normally found in neutral or slightly acidic hydrothermal systems, was detected in microbial communities. As the diatom concentration increases, so does the dry matter concentration in mats, while the content of organic matter decreases. The concentrations of proteins and carbohydrates reached their maximum levels at 45-50 degrees C. The maximum average rate of oxygenic photosynthesis (2.1 g C/m2 day), chlorophyll a content (343.4 mg/m2), and cell number of phototrophic microorganisms were observed at temperatures from 45 to 50 degrees C. The peak mass of bacterial mats (56.75 g/m2) occurred at a temperature of 65-60 degrees C. The maximum biomass concentration of phototrophs (414.63 x 10(-6) g/ml) and the peak rate of anoxygenic photosynthesis [0.42 g C/(m2 day)] were observed at a temperature of 35-40 degrees C.

This article appeared in a journal published by Elsevier. The attached copy is furnished to the a... more This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues. Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. In most cases authors are permitted to post their version of the article (e.g. in Word or Tex form) to their personal website or institutional repository. Authors requiring further information regarding Elsevier's archiving and manuscript policies are encouraged to visit: http://www.elsevier.com/copyright

Doklady Earth Sciences

Data indicating the important role of microorganisms in the redistribution of REEs in the weather... more Data indicating the important role of microorganisms in the redistribution of REEs in the weathering crust and the decisive role in the concentration of REEs during the formation of ores in the upper ore horizon of the Tomtor field are obtained. The uptake of REEs was carried out by the community of microorganisms, such as phototrophs, methanogens, methanotrophs, and proteobacteria, which form the basis of the microbiocenosis for this paleoecosystem. The isotopic composition of C carbonates in all samples studied with fossilized microorganisms corresponds to the biogenic one, and the isotopic composition δ18ОSMOW (from 7 to 20‰) indicates the endogenous (hydrothermal) and, to a lesser extent, exogenous nature of the solutions. The low (87Sr/86Sr)I values of carbonates (~0.7036–0.7042) exclude the participation of seawater.