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Papers by A. Kondic-spika
Pesticides having different structures and biological activities are widely used for agricultural... more Pesticides having different structures and biological activities are widely used for agricultural and non-agricultural purposes. Due to their widespread use, pesticides need to be determined in various environmental matrices. A wide range of analytical techniques have been developed in order to identify the organic contaminants often present at trace levels in environmental samples. The aim of this work was to develop a reliable, rapid, robust and cost-effective method for the determination of five sulfonylurea herbicides (nicosulfuron, oxasulfuron, tribenuron methyl, triasulfuron and tritosulfuron) in spiked river water samples. The samples were diluted with 0.5% acetic acid (1:1) and purified by solid-phase extraction (SPE) on Oasis MAX cartridges. After the cartridges were sequentially washed with water, methanol, ethyl acetate, and acetonitrile, they were eluted with 0.1% formic acid in acetonitrile. Elutes were evaporated to dryness and reconstituted in acetonitrile. The purifi...
Archives of Biological Sciences, 2009
Tolerance of wheat genotypes to the sulfonylurea herbicide metsulfuron-methyl was studied using i... more Tolerance of wheat genotypes to the sulfonylurea herbicide metsulfuron-methyl was studied using in vitro culture. Six randomly selected wheat genotypes were used for isolation. Mature embryos were inoculated onto a modified MS medium to which three concentrations of metsulfuron-methyl were added: I-30 g l-1, II-60 g l-1, and III-90 g l-1. The control group of embryos was cultivated on a herbicide-free medium. After one month of cultivation, callus fresh weight was measured. There were significant differences among the genotypes regarding their tolerance to metsulfuron-methyl. The Pobeda cultivar expressed the highest and the Lr-12 genotype had the lowest level of herbicide tolerance.
Czech Journal of Genetics and Plant Breeding
One of the main wheat breeder’s goals is determining specific genomic regions which control impor... more One of the main wheat breeder’s goals is determining specific genomic regions which control important agronomical traits. Association analysis is a new strategy with high resolution in plant molecular breeding that could be used to improve the efficiency of marker assisted selection (MAS) for finding important QTLs (quantitative trait loci) or genes. A set of 96 diverse wheat genotypes was phenotypically measured during three growing seasons (2006/07, 2007/08, 2008/09). Microsatellite markers located near important QTLs were carefully chosen in accordance with existing literature data to validate marker trait associations (MTA). Genomic DNA was extracted using the CTAB method and PCR products were separated by capillary electrophoresis. The population structure was assigned based on molecular data in Structure v. 2.0 software, while association analysis was done by the Tassel program using the Q matrix. Nine significant associations were stable in all years investigated and eight MT...
Genetika, 2011
More effective breeding and development of new wheat genotypes depend on an intricate analysis of... more More effective breeding and development of new wheat genotypes depend on an intricate analysis of the complex relationships among many different traits. The objective of this paper was to determine the interrelationship, direct and indirect effects, and stability of different yield components in wheat. Forty divergent genotypes were analyzed in a three- year study (2005-2007). Highly significant correlations were found between grain yield per plant and all the other traits analyzed except spike length, with the only negative correlation being that with plant height. Path analysis revealed highly significant direct effects of grain number per spike, grain mass per spike and 1000 grain weight on grain yield per plant. Analysis of stability parameters showed that the stability of grain yield per plant depended for the most part on the stability of grain number per spike, grain mass per spike and harvest index. Cluster analysis identified genotypes with a high performance for grain yiel...
Ratarstvo i povrtarstvo, 2013
Gluten content, and more importantly its composition expressed through glutenin subunits, has gre... more Gluten content, and more importantly its composition expressed through glutenin subunits, has great influence on the rheological and bread-making properties of wheat flour. Total of 168 winter wheat cultivars developed at the Institute of Field and Vegetable Crops, Novi Sad, Serbia in the period 1987-2008 were analysed for high molecular weight glutenin subunits (HMW-GS) composition using SDS-PAGE. Presence of twelve different alleles and nineteen different GS combinations was determined. The highest frequency was found for GS N at the locus Glu-A1 (46%), 7+9 (77%) at the locus Glu-B1 and 5+10 (72.7%) at the locus Glu-D1. The most frequent combination was 2*, 7+9, 5+10. Presence of several rare GS with positive effect (13+16 and 15+16) was determined, as well as high uniformity of the genetic material, considering small number of cultivars (4.2%) with different electrophoretic paths. Two modes of glutenin score (Glu-1 score) determination were applied, based on which differences in bread-making quality among individual cultivars can be determined more precisely.
Genetika, 2007
Androgenous and regeneration abilities of 14 randomly selected F1 hybrids of wheat (Triticum aest... more Androgenous and regeneration abilities of 14 randomly selected F1 hybrids of wheat (Triticum aestivum L.) were analyzed. Anthers were grown in vitro on a modified Potato-2 inductive medium. The hybrid NS111-95/Ana had the highest average values for androgenous capacity (33%) and callus yield (119%), while the hybrid NS 92-250/Tiha had the lowest values for these traits (9 and 21%, respectively). Seven genotypes (50%) had a frequency of green plants relative to the number of isolated anthers of over 10%, with the highest frequency of 21.3% (NS111-95/Sremica). This hybrid produced 12.8 doubled haploid (DH) lines per spike used for isolation. In the other genotypes, the number of produced DH lines per spike ranged from 1 (30?Sc.Smoc.88-89/Hays-2) to 11.2 (NS111-95/Ana). As half of the randomly selected genotypes exhibited high green plant regeneration ability and a high production of DH lines per spike, it can be concluded that in vitro anther culture can be successfully used in breedi...
… o biotehnologiji, 15, …, 2010
... The standpoint varieties were Evropa 90 and Prima, which proved the most stable and had above... more ... The standpoint varieties were Evropa 90 and Prima, which proved the most stable and had above-average mean values. The cultivar Milica exhibited good stability and had high average values as well. ... 21000 Novi Sad. Contact: Zorka VESOVIC. Tel: +381-21-525 859. ...
Euphytica, 2010
The objective of this study was to determine genotype 9 environment interactions (GEI) obtained i... more The objective of this study was to determine genotype 9 environment interactions (GEI) obtained in wheat production in southeast Europe for protein content, sedimentation value, and loaf volume. Twenty wheat genotypes divided in two groups with different combinations of high molecular weight glutenin subunits (HMW-GS) were analyzed in 15 environments (E). In a combined analysis of variance, effects of genotype, environment, and their interaction (GEI) were highly significant for almost all the analyzed traits. All the three traits were influenced more by location than by year, with protein content and sedimentation value being more responsive to changes in environmental conditions than loaf volume. The genotypes with high protein content had the regression coefficient value (b) close to 1, indicating that they did not react to extreme environmental conditions as was the case with genotypes with high sedimentation and loaf volume values. There were no significant differences in average values for the analyzed traits and adaptability parameters between the two groups of genotypes. It means that, concerning wheat quality improvement, genotypes with different HMW-GSs on the Glu-1D locus could be recommended for growing in southeast Europe. It is necessary to take into account the differences in adaptability of protein content to either positive or negative environmental changes that were observed between the groups.
Cereal Research Communications, 2010
Pesticides having different structures and biological activities are widely used for agricultural... more Pesticides having different structures and biological activities are widely used for agricultural and non-agricultural purposes. Due to their widespread use, pesticides need to be determined in various environmental matrices. A wide range of analytical techniques have been developed in order to identify the organic contaminants often present at trace levels in environmental samples. The aim of this work was to develop a reliable, rapid, robust and cost-effective method for the determination of five sulfonylurea herbicides (nicosulfuron, oxasulfuron, tribenuron methyl, triasulfuron and tritosulfuron) in spiked river water samples. The samples were diluted with 0.5% acetic acid (1:1) and purified by solid-phase extraction (SPE) on Oasis MAX cartridges. After the cartridges were sequentially washed with water, methanol, ethyl acetate, and acetonitrile, they were eluted with 0.1% formic acid in acetonitrile. Elutes were evaporated to dryness and reconstituted in acetonitrile. The purifi...
Archives of Biological Sciences, 2009
Tolerance of wheat genotypes to the sulfonylurea herbicide metsulfuron-methyl was studied using i... more Tolerance of wheat genotypes to the sulfonylurea herbicide metsulfuron-methyl was studied using in vitro culture. Six randomly selected wheat genotypes were used for isolation. Mature embryos were inoculated onto a modified MS medium to which three concentrations of metsulfuron-methyl were added: I-30 g l-1, II-60 g l-1, and III-90 g l-1. The control group of embryos was cultivated on a herbicide-free medium. After one month of cultivation, callus fresh weight was measured. There were significant differences among the genotypes regarding their tolerance to metsulfuron-methyl. The Pobeda cultivar expressed the highest and the Lr-12 genotype had the lowest level of herbicide tolerance.
Czech Journal of Genetics and Plant Breeding
One of the main wheat breeder’s goals is determining specific genomic regions which control impor... more One of the main wheat breeder’s goals is determining specific genomic regions which control important agronomical traits. Association analysis is a new strategy with high resolution in plant molecular breeding that could be used to improve the efficiency of marker assisted selection (MAS) for finding important QTLs (quantitative trait loci) or genes. A set of 96 diverse wheat genotypes was phenotypically measured during three growing seasons (2006/07, 2007/08, 2008/09). Microsatellite markers located near important QTLs were carefully chosen in accordance with existing literature data to validate marker trait associations (MTA). Genomic DNA was extracted using the CTAB method and PCR products were separated by capillary electrophoresis. The population structure was assigned based on molecular data in Structure v. 2.0 software, while association analysis was done by the Tassel program using the Q matrix. Nine significant associations were stable in all years investigated and eight MT...
Genetika, 2011
More effective breeding and development of new wheat genotypes depend on an intricate analysis of... more More effective breeding and development of new wheat genotypes depend on an intricate analysis of the complex relationships among many different traits. The objective of this paper was to determine the interrelationship, direct and indirect effects, and stability of different yield components in wheat. Forty divergent genotypes were analyzed in a three- year study (2005-2007). Highly significant correlations were found between grain yield per plant and all the other traits analyzed except spike length, with the only negative correlation being that with plant height. Path analysis revealed highly significant direct effects of grain number per spike, grain mass per spike and 1000 grain weight on grain yield per plant. Analysis of stability parameters showed that the stability of grain yield per plant depended for the most part on the stability of grain number per spike, grain mass per spike and harvest index. Cluster analysis identified genotypes with a high performance for grain yiel...
Ratarstvo i povrtarstvo, 2013
Gluten content, and more importantly its composition expressed through glutenin subunits, has gre... more Gluten content, and more importantly its composition expressed through glutenin subunits, has great influence on the rheological and bread-making properties of wheat flour. Total of 168 winter wheat cultivars developed at the Institute of Field and Vegetable Crops, Novi Sad, Serbia in the period 1987-2008 were analysed for high molecular weight glutenin subunits (HMW-GS) composition using SDS-PAGE. Presence of twelve different alleles and nineteen different GS combinations was determined. The highest frequency was found for GS N at the locus Glu-A1 (46%), 7+9 (77%) at the locus Glu-B1 and 5+10 (72.7%) at the locus Glu-D1. The most frequent combination was 2*, 7+9, 5+10. Presence of several rare GS with positive effect (13+16 and 15+16) was determined, as well as high uniformity of the genetic material, considering small number of cultivars (4.2%) with different electrophoretic paths. Two modes of glutenin score (Glu-1 score) determination were applied, based on which differences in bread-making quality among individual cultivars can be determined more precisely.
Genetika, 2007
Androgenous and regeneration abilities of 14 randomly selected F1 hybrids of wheat (Triticum aest... more Androgenous and regeneration abilities of 14 randomly selected F1 hybrids of wheat (Triticum aestivum L.) were analyzed. Anthers were grown in vitro on a modified Potato-2 inductive medium. The hybrid NS111-95/Ana had the highest average values for androgenous capacity (33%) and callus yield (119%), while the hybrid NS 92-250/Tiha had the lowest values for these traits (9 and 21%, respectively). Seven genotypes (50%) had a frequency of green plants relative to the number of isolated anthers of over 10%, with the highest frequency of 21.3% (NS111-95/Sremica). This hybrid produced 12.8 doubled haploid (DH) lines per spike used for isolation. In the other genotypes, the number of produced DH lines per spike ranged from 1 (30?Sc.Smoc.88-89/Hays-2) to 11.2 (NS111-95/Ana). As half of the randomly selected genotypes exhibited high green plant regeneration ability and a high production of DH lines per spike, it can be concluded that in vitro anther culture can be successfully used in breedi...
… o biotehnologiji, 15, …, 2010
... The standpoint varieties were Evropa 90 and Prima, which proved the most stable and had above... more ... The standpoint varieties were Evropa 90 and Prima, which proved the most stable and had above-average mean values. The cultivar Milica exhibited good stability and had high average values as well. ... 21000 Novi Sad. Contact: Zorka VESOVIC. Tel: +381-21-525 859. ...
Euphytica, 2010
The objective of this study was to determine genotype 9 environment interactions (GEI) obtained i... more The objective of this study was to determine genotype 9 environment interactions (GEI) obtained in wheat production in southeast Europe for protein content, sedimentation value, and loaf volume. Twenty wheat genotypes divided in two groups with different combinations of high molecular weight glutenin subunits (HMW-GS) were analyzed in 15 environments (E). In a combined analysis of variance, effects of genotype, environment, and their interaction (GEI) were highly significant for almost all the analyzed traits. All the three traits were influenced more by location than by year, with protein content and sedimentation value being more responsive to changes in environmental conditions than loaf volume. The genotypes with high protein content had the regression coefficient value (b) close to 1, indicating that they did not react to extreme environmental conditions as was the case with genotypes with high sedimentation and loaf volume values. There were no significant differences in average values for the analyzed traits and adaptability parameters between the two groups of genotypes. It means that, concerning wheat quality improvement, genotypes with different HMW-GSs on the Glu-1D locus could be recommended for growing in southeast Europe. It is necessary to take into account the differences in adaptability of protein content to either positive or negative environmental changes that were observed between the groups.
Cereal Research Communications, 2010