Angela Lucaccioni - Academia.edu (original) (raw)

Angela Lucaccioni

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Papers by Angela Lucaccioni

Research paper thumbnail of La starvation per l’adenina e la mancanza dell’attività proofreading della polimerasi delta causano altissime frequenze di mutazione nel genoma di S.cerevisiae

Research paper thumbnail of Colony density influences invasive and filamentous growth inSaccharomyces cerevisiae

Folia Microbiologica, 2007

Research paper thumbnail of La starvation per l’adenina e la mancanza dell’attività proofreading della polimerasi delta causano altissime frequenze di mutazione nel genoma di S.cerevisiae

Research paper thumbnail of Adaptive Mutagenesis in the Yeast Saccharomyces Cerevisiae

Ecological genetics, 2006

The nature of mutation in microorganisms has been debated for a long time. Two theories have been... more The nature of mutation in microorganisms has been debated for a long time. Two theories have been at odds: random spontaneous mutagenesis vs. adaptive mutagenesis. "random mutagenesis" means that mutations occur in proliferating cells before they encountered the selective agent. "adaptive mutagenesis" means that advantageous mutations form in the environment where they have been selected, in non-replicating or poorly replicating cells even though other, non-selected, mutations occur at the same time. In the last 20 years it has been definitely shown that random as well as adaptive mutagenesis occur in bacteria and yeast. microorganisms in nature do not divide or divide poorly because of adverse environmental conditions; therefore adaptive mutations could provide cells with a selective advantage and allow evolution of populations. Here we will focus on some fundamental aspects of adaptive mutagenesis in the yeast Saccharomyces cerevisiae. We begin with a historica...

Research paper thumbnail of Colony density influences invasive and filamentous growth inSaccharomyces cerevisiae

Folia Microbiologica, 2007

Research paper thumbnail of High rate of starvation-associated mutagenesis in Ung− yeast caused by the overproduction of human activation-induced deaminase

Research paper thumbnail of The exceptionally high rate of spontaneous mutations in the polymerase delta proofreading exonuclease-deficient Saccharomyces cerevisiae strain starved for adenine

BMC genetics, Jan 23, 2004

Mutagenesis induced in the yeast Saccharomyces cerevisiae by starvation for nutrilites is a well-... more Mutagenesis induced in the yeast Saccharomyces cerevisiae by starvation for nutrilites is a well-documented phenomenon of an unknown mechanism. We have previously shown that the polymerase delta proofreading activity controls spontaneous mutagenesis in cells starved for histidine. To obtain further information, we compared the effect of adenine starvation on mutagenesis in wild-type cells and, in cells lacking the proofreading activity of polymerase delta (phenotype Exo-, mutation pol3-01). Ade+ revertants accumulated at a very high rate on adenine-free plates so that their frequency on day 16 after plating was 1.5 x 10(-4) for wild-type and 1.0 x 10(-2) for the Exo- strain. In the Exo- strain, all revertants arising under adenine starvation are suppressors of the original mutation, most possessed additional nutritional requirements, and 50% of them were temperature sensitive. Adenine starvation is highly mutagenic in yeast. The deficiency in the polymerase delta proofreading activi...

Research paper thumbnail of La starvation per l’adenina e la mancanza dell’attività proofreading della polimerasi delta causano altissime frequenze di mutazione nel genoma di S.cerevisiae

Research paper thumbnail of Colony density influences invasive and filamentous growth inSaccharomyces cerevisiae

Folia Microbiologica, 2007

Research paper thumbnail of La starvation per l’adenina e la mancanza dell’attività proofreading della polimerasi delta causano altissime frequenze di mutazione nel genoma di S.cerevisiae

Research paper thumbnail of Adaptive Mutagenesis in the Yeast Saccharomyces Cerevisiae

Ecological genetics, 2006

The nature of mutation in microorganisms has been debated for a long time. Two theories have been... more The nature of mutation in microorganisms has been debated for a long time. Two theories have been at odds: random spontaneous mutagenesis vs. adaptive mutagenesis. "random mutagenesis" means that mutations occur in proliferating cells before they encountered the selective agent. "adaptive mutagenesis" means that advantageous mutations form in the environment where they have been selected, in non-replicating or poorly replicating cells even though other, non-selected, mutations occur at the same time. In the last 20 years it has been definitely shown that random as well as adaptive mutagenesis occur in bacteria and yeast. microorganisms in nature do not divide or divide poorly because of adverse environmental conditions; therefore adaptive mutations could provide cells with a selective advantage and allow evolution of populations. Here we will focus on some fundamental aspects of adaptive mutagenesis in the yeast Saccharomyces cerevisiae. We begin with a historica...

Research paper thumbnail of Colony density influences invasive and filamentous growth inSaccharomyces cerevisiae

Folia Microbiologica, 2007

Research paper thumbnail of High rate of starvation-associated mutagenesis in Ung− yeast caused by the overproduction of human activation-induced deaminase

Research paper thumbnail of The exceptionally high rate of spontaneous mutations in the polymerase delta proofreading exonuclease-deficient Saccharomyces cerevisiae strain starved for adenine

BMC genetics, Jan 23, 2004

Mutagenesis induced in the yeast Saccharomyces cerevisiae by starvation for nutrilites is a well-... more Mutagenesis induced in the yeast Saccharomyces cerevisiae by starvation for nutrilites is a well-documented phenomenon of an unknown mechanism. We have previously shown that the polymerase delta proofreading activity controls spontaneous mutagenesis in cells starved for histidine. To obtain further information, we compared the effect of adenine starvation on mutagenesis in wild-type cells and, in cells lacking the proofreading activity of polymerase delta (phenotype Exo-, mutation pol3-01). Ade+ revertants accumulated at a very high rate on adenine-free plates so that their frequency on day 16 after plating was 1.5 x 10(-4) for wild-type and 1.0 x 10(-2) for the Exo- strain. In the Exo- strain, all revertants arising under adenine starvation are suppressors of the original mutation, most possessed additional nutritional requirements, and 50% of them were temperature sensitive. Adenine starvation is highly mutagenic in yeast. The deficiency in the polymerase delta proofreading activi...

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