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Papers by ANA CAROLINA HURTADO GONZALES
Arthrogryposis multiplex congenita is a rare non- progressive syndrome consists of a heterogeneou... more Arthrogryposis multiplex congenita is a rare non- progressive syndrome consists of a heterogeneous group of congenital malformations of unknown etiology, multifactorial, mainly characterized by multiple joint contractures. Objective: to evaluate the importance of physiotherapy performance in patients with arthrogryposis multiplex congenita, as well as treatment methods and physical-motor and psychosocial benefits. Method: this study consists of a systematic review of articles in which search was conducted in databases like PubMed, LILACS, SciELO. The keywords used in the search were Arthrogryposis multiplex congenita and Physiotherapy, without language restriction. Results and final considerations: in total seven articles met all inclusion criteria. Only four reported a physical therapy evaluation. A varied number of intervention techniques and results were described. This systematic review demonstrated the importance of physiotherapy performance in patients with arthrogryposis mult...
Biomarker Insights, 2007
Background Pesticides are of concern in Bolivia because of increasing use. Frequent intoxications... more Background Pesticides are of concern in Bolivia because of increasing use. Frequent intoxications have been demonstrated due to use of very toxic pesticides, insufficient control of distribution and sale and little knowledge among farmers of protective measures and hygienic procedures. Method Questionnaires were applied and blood tests taken from 81 volunteers from La Paz County, of whom 48 were pesticide exposed farmers and 33 non-exposed controls. Sixty males and 21 females participated with a mean age of 37.3 years (range 17–76). Data of exposure and possible genetic damage were collected and evaluated by well known statistical methods, controlling for relevant confounders. To measure genetic damage chromosomal aberrations and the comet assay analysis were performed. Results Pesticide exposed farmers had a higher degree of genetic damage compared to the control group. The number of chromosomal aberrations increased with the intensity of pesticide exposure. Females had a lower num...
Jornal Brasileiro de Psiquiatria, 2012
OBJETIVO: Analisar o perfil de humor e fatores associados em participantes de um programa de reab... more OBJETIVO: Analisar o perfil de humor e fatores associados em participantes de um programa de reabilitação cardiopulmonar e metabólica. MÉTODO: Cento e quarenta e quatro pacientes responderam a um questionário de caracterização e à Escala de Humor de Brunel (BRUMS). O questionário de caracterização foi constituído de perguntas sobre dados pessoais e clínicos, enquanto o BRUMS avaliou o humor por meio de seis fatores: tensão, depressão, raiva, vigor, fadiga e confusão. Os dados foram tratados com estatística descritiva e inferencial (α = 0,05). RESULTADOS: De maneira geral, os pacientes com doenças cardiovasculares e metabólicas apresentaram boa saúde mental, com níveis elevados de vigor, associados à moderada tensão, depressão, raiva, fadiga e confusão. Os participantes idosos apresentaram menor tensão, raiva, fadiga e confusão do que os adultos, enquanto os participantes com dislipidemia tinham maior tensão. Aqueles com insuficiência cardíaca eram mais tensos e fadigados. Quem fuma ...
Revista Cientifica Ciencia Medica, 2014
Las infecciones nosocomiales constituyen un factor que aumenta la morbimortalidad sobre todo en e... more Las infecciones nosocomiales constituyen un factor que aumenta la morbimortalidad sobre todo en el paciente inmunosuprimido como los infantiles quemados. Es por ello que este trabajo tiene como objetivo identificar Pseudomonas aeruginosa y Staphylococcus aureus en el pabellon infantil de quemados del Hospital Viedma. El estudio es de tipo descriptivo transversal, el universo fue el pabellon infantil de quemados y la muestra los materiales y equipos que esten en frecuente contacto con los ninos quemados: Batas de visita, grifo del bano, manguera del equipo de suero, agarrador de cama, sabanas, manija de la puerta, sillon, paredes, agua de hidroterapia, tina de hidroterapia, frazadas esteriles, estantes, tina de bano, balanza, mascara de respirador, uniforme del personal de salud, manos de las enfermeras y medio ambiente. Se realizo un hisopado, se sembro en Agar Sangre y Agar Mckoney para identificar Staphylococcus aureus y Pseudomonas aeruginosa adicionado el metodo de la coagulasa en las colonias de Staphylococcus aureus; posteriormente se realizo el perfil antimicrobiano. Los resultados evidenciaron la identificacion de Staphylococcus aureus, no asi de Pseudomonas aeruginosa, en frazadas, sabanas, frazadas esteriles y manos del personal de salud. El antibiograma dio como resultado que son resistentes a la Meticilina y la colonia identificada en las frazadas esteriles tambien es resistente a Vancomicina. Se concluye que Staphylococcus aureus coloniza varios elementos del pabellon de quemados, por otro lado hay que tener en cuenta su perfil de resistencia para tratar infecciones por esta causa dentro el pabellon de quemados del Hospital Viedma
Rbm Rev Bras Med, May 1, 2013
Em pacientes com Aids a terapia antirretroviral de alta potencia (TARV) costuma causar disturbios... more Em pacientes com Aids a terapia antirretroviral de alta potencia (TARV) costuma causar disturbios nos sistemas imunologico, cardiorrespiratorio e muscular, o que pode ser minimizado pelo treinamento fisico e suplementacao com hormonio anabolizante. Objetivo: Examinar na literatura as evidencias sobre a influencia do treinamento fisico e a suplementacao com hormonio anabolizante sobre as funcoes imunologica, cardiorrespiratoria e muscular, na populacao infectada pelo virus HIV. Metodo: Revisao sistematica nas bases de dados SciELO, PUBMED, LILACS, MEDLINE, COCHRANE DATABASE, utilizando os seguintes descritores: HIV/Aids and antiretroviral treatment, exercise training, physical activity and HIV, physical activity and testosterone, testosterone and HIV, anabolic steroids, immune system, cardiorespiratory system, muscular system e seus respectivos na lingua portuguesa. Foram incluidas publicacoes desde 2000, que fossem meta-analise, ensaio clinico controlado, estudo epidemiologico do tipo coorte, estudo epidemiologico do tipo caso-controle ou revisao sistematica que avaliassem a influencia do exercicio fisico e dos hormonios anabolicos sobre as funcoes imunologica, cardiorrespiratoria e muscular. Resultados e conclusoes: Foram encontrados 14 artigos, demonstrando a melhora das funcoes cardiorrespiratoria, imunologica ou muscular, decorrente do exercicio fisico isolado, sendo que o uso exclusivo de anabolizante ou associado ao exercicio contribuiu apenas para ganho adicional de massa magra, conforme relatado em dois artigos
Cartilla pedagógica de actualidad internacional taller: un mundo más allá de nuestras fronteras. ... more Cartilla pedagógica de actualidad internacional taller: un mundo más allá de nuestras fronteras. Realizado durante el primer semestre de 2007 en la localidad de Suba / Ana Cecilia Burgos González...[et. al].
Brazilian Journal of Biomechanics Revista Brasileira De Biomecânica, Jun 25, 2013
A distrofia miotonica de Steinert (DMS) e a forma mais frequente de distrofia muscular em adultos... more A distrofia miotonica de Steinert (DMS) e a forma mais frequente de distrofia muscular em adultos, sendo o acometimento da musculatura respiratoria sua principal causa de mortalidade. Na DMS o musculo diafragma encontra-se enfraquecido em decorrencia da evolucao da doenca e os musculos esternocleidomastoideo (ECM) e trapezio superior sao ativados como forma compensatoria da respiracao. A eletromiografia (EMG) e uma ferramenta muito utilizada no diagnostico DMS, porem sua incidencia tem se mostrado variada devido ao grande numero de musculos estudados e a interpretacao dos achados diferenciados. Para permitir uma comparacao entre os estudos torna-se relevante a normalizacao dos dados. Objetivo principal: Analisar em pacientes portadores de DMS diferentes procedimentos de normalizacao da amplitude EMG dos musculos ECM e trapezio superior durante a ativacao destes na fase inspiratoria. Materiais e Metodos: Foram avaliados quatro pacientes do sexo feminino, com idade entre 18 e 44 anos atendidas em instituicao de Portadores de Distrofias Musculares Progressivas. Os dados EMG foram coletados em quatro posicionamentos diferentes para cada musculo estudado quantificados em RMS (raiz quadrada da media) e expressos em microvolts. Resultados: As CIVR (Contracao Voluntaria de Referencia) de elevacao de membros superiores ao ângulo de 60° com carga de 500gr nas maos, e elevacao do pescoco em posicao de deitada, foram aquelas que apresentaram menor variabilidade. Conclusao: a atividade respiratoria de pacientes com DMS pode ser normalizada no entanto, devem ser realizados novos estudos com maior numero de sujeitos a fim de verificar a confirmacao dos achados eletromiograficos encontrados.
Revista Brasileira de Atividade Física & Saúde, 2014
The infant juvenile obesity is currently one of the most serious public health problems of the 21... more The infant juvenile obesity is currently one of the most serious public health problems of the 21st century, being directly related to physical inactivity as a decisive factor in the worldwide epidemic. The programs of conventional workouts have become not so attractive for this particular population. Because of that the access and permanence in those structured physical activity programs for obese adolescents are difficult. This systematic review has the general goal to verify what benefits the systematic practice of hip hop dance, can promote in obese children and adolescents. The search for relevant articles was conducted in the electronic databases Medline (Pubmed), Cochrane, LILACS, SciELO from January through June 2014, being structured as [(hip hop /) and (obesity /) and (children/ adolescent), controlled and randomized trials were included regarding the systematic practice of hip hop dance as exercise and its benefits for obese children and adolescents, subjects aged 2-19, considered obese and/ or overweight. A total of 21 articles were identified in the survey. Based on the found eligible articles for the full review, only one article met all the inclusion criteria. This preliminary study is intended to broaden the context in health for adolescents and children suffering from overweight and/or obesity, recommending an intervention through dance hip hop, it would be an important modality association for possible monitoring and reducing the risk of associated comorbidities. In this field of research, there is a lack of studies of high methodological quality, which may be able to demonstrate the benefits of this type of intervention.
The American journal of otology, 2000
This study aimed to analyze the localization and distribution of vessels and of these angiogenic ... more This study aimed to analyze the localization and distribution of vessels and of these angiogenic growth factors: basic fibroblast growth factor (FGF-2), transforming growth factor-alpha (TGF-alpha), transforming growth factor-beta1 (TGF-beta1), and vascular endothelial growth factor (VEGF) in middle ear cholesteatoma in comparison with normal middle ear mucosa and auditory meatal skin. Angiogenesis is particularly important in many normal and pathologic processes, including wound healing and inflammation. Because proliferating tissues require an enhanced blood supply, angiogenesis appears to be a prerequisite for the expansion of cholesteatoma. The expression of FGF-2, TGF-alpha, TGF-beta1, and VEGF was studied by immunohistochemistry. The amount of vessels (collagen type IV staining) was determined by an automatic imaging analyzing system. The results showed an altered expression and distribution of VEGF, FGF-2, TGF-alpha, and TGF-beta1 in cholesteatoma in relation to middle ear mu...
Brazilian Journal of Physical Therapy, 2014
Background: Patients with heart failure (HF) usually develop exercise intolerance. In this contex... more Background: Patients with heart failure (HF) usually develop exercise intolerance. In this context, noninvasive ventilation (NIV) can help to increase physical performance. Objective: To undertake a systematic review and meta-analysis of randomized controlled trials that evaluated the effects of NIV on exercise tolerance in patients with HF. Method: Search Strategy: Articles were searched in the following databases: Physiotherapy Evidence Database (PEDro), Scientific Electronic Library Online (SciELO), and MEDLINE. Selection Criteria: This review included only randomized controlled trials involving patients with HF undergoing NIV, with or without other therapies, that used exercise tolerance as an outcome, verified by the distance travelled in the six-minute walk test (6MWT), VO 2 peak in the cardiopulmonary test, time spent in testing, and dyspnea. Data Collection and Analysis: The methodological quality of the studies was rated according to the PEDro scale. Data were pooled in fixed-effect meta-analysis whenever possible. Results: Four studies were selected. A meta-analysis including 18 participants showed that the use of NIV prior to the 6MWT promoted increased distance, [mean difference 65.29 m (95% CI 38.80 to 91.78)]. Conclusions: The use of NIV prior to the 6MWT in patients with HF may promote increased distance. However, the limited number of studies may have compromised a more definitive conclusion on the subject.
Revista Brasileira de Medicina do Esporte, 2012
O presente estudo teve por objetivo traduzir e validar para a língua portuguesa o Questionário Nu... more O presente estudo teve por objetivo traduzir e validar para a língua portuguesa o Questionário Nutricional Simplificado de Apetite (QNSA), verificando sua clareza e validade para participantes de programa de Reabilitação Cardiopulmonar e Metabólica (RCPM). Cento e quarenta e seis indivíduos foram entrevistados, sendo 79 homens, com média de idade de 63 anos (± 10) e 67 mulheres, com média de 66 anos (± 11). A análise descritiva foi apresentada em média, desvio padrão e frequência. A consistência interna da escala foi avaliada por meio do coeficiente alfa de Cronbach e a análise fatorial pelo método de extração de análise dos componentes principais utilizando os critérios de Kaiser (autovalores > 1). Todas as questões do instrumento apresentaram resultados positivos em relação à clareza. Os itens do questionário apresentaram carga fatorial superior a 0,40, com variação de 0,40 a 0,81. Com exceção da questão relacionada com a quantidade de refeições diárias, todas as demais apresen...
Revista Brasileira de Medicina do Esporte, 2014
INTRODUÇÃO: Os programas de reabilitação cardiopulmonar e metabólica (RCPM) vêm adotando protocol... more INTRODUÇÃO: Os programas de reabilitação cardiopulmonar e metabólica (RCPM) vêm adotando protocolos de treinamento físico que valorizam o volume e a alta intensidade, sendo possível o aparecimento da síndrome do excesso de treinamento (SET). Neste contexto, o uso sistemático de instrumentos para investigação do estado de humor pode auxiliar na detecção precoce da SET.OBJETIVO: Validar a escala de Humor de Brunel (BRUMS) em participantes de RCPM.MÉTODOS: Fizeram parte do estudo 247 indivíduos, com média de idade de 63,3±10,5 anos, participantes há pelo menos três meses de programa de RCPM. A análise descritiva foi apresentada em média, desvio padrão e distribuição de frequência. Para avaliar a validade de construto foi utilizada a análise fatorial confirmatória. Para verificar a adequação dos dados foi utilizado o índice de Kaiser-Meyer-Olkin (KMO). Para interpretação da matriz, foi aplicado o método de extração dos componentes principais, por meio do método varimax. A consistência f...
G3 (Bethesda, Md.), Jan 22, 2014
Genetic differentiation in natural populations is driven by geographic distance and by ecological... more Genetic differentiation in natural populations is driven by geographic distance and by ecological or physical features within and between natural habitats that reduce migration. The primary population structure in wild barley differentiates populations east and west of the Zagros Mountains. Genetic differentiation between eastern and western populations is uneven across the genome and is greatest on linkage groups 2H and 5H. Genetic markers in these two regions demonstrate the largest difference in frequency between the primary populations and have the highest informativeness for assignment to each population. Previous cytological and genetic studies suggest there are chromosomal structural rearrangements (inversions or translocations) in these genomic regions. Environmental association analyses identified an association with both temperature and precipitation variables on 2H and with precipitation variables on 5H.
The Plant Journal, 2013
Advanced resources for genome-assisted research in barley (Hordeum vulgare) including a whole-gen... more Advanced resources for genome-assisted research in barley (Hordeum vulgare) including a whole-genome shotgun assembly and an integrated physical map have recently become available. These have made possible studies that aim to assess genetic diversity or to isolate single genes by whole-genome resequencing and in silico variant detection. However such an approach remains expensive given the 5 Gb size of the barley genome. Targeted sequencing of the mRNA-coding exome reduces barley genomic complexity more than 50-fold, thus dramatically reducing this heavy sequencing and analysis load. We have developed and employed an in-solution hybridization-based sequence capture platform to selectively enrich for a 61.6 megabase coding sequence target that includes predicted genes from the genome assembly of the cultivar Morex as well as publicly available full-length cDNAs and de novo assembled RNA-Seq consensus sequence contigs. The platform provides a highly specific capture with substantial and reproducible enrichment of targeted exons, both for cultivated barley and related species. We show that this exome capture platform provides a clear path towards a broader and deeper understanding of the natural variation residing in the mRNA-coding part of the barley genome and will thus constitute a valuable resource for applications such as mapping-by-sequencing and genetic diversity analyzes.
Journal of Heredity, 2012
The common morning glory (Ipomoea purpurea) is an annual vine native to Central and Southern Mexi... more The common morning glory (Ipomoea purpurea) is an annual vine native to Central and Southern Mexico. The genetics of flower color polymorphisms and interactions with the biotic environment have been extensively studied in I. purpurea and in its sister species I. nil. In this study, we examine nucleotide sequence polymorphism in 11 loci, 9 of which are known to participate in a pathway that produces floral pigments. A sample of 30 I. purpurea accessions from the native range of Central and Southern Mexico comprise the data, along with one accession from each of the two sister species I. alba and I. nil. We observe moderate levels of nucleotide sequence polymorphism of ~1%. The ratio of recombination to mutation parameter estimates (ρ/θ) of ~2.5 appears consistent with a mixed-mating system. Ipomoea resequencing data from these genic regions are noteworthy in providing a good fit to the standard neutral model of molecular evolution. The derived silent site frequency spectrum is very close to that predicted by coalescent simulations of a drift-mutation process, and Tajima's D values are not significantly different from expectations under neutrality.
Journal of Heredity, 2013
The levels of diversity and extent of linkage disequilibrium in cultivated species are largely de... more The levels of diversity and extent of linkage disequilibrium in cultivated species are largely determined by diversity in their wild progenitors. We report a comparison of nucleotide sequence diversity in wild and cultivated barley (Hordeum vulgare ssp. spontaneum and ssp. vulgare) at 7 nuclear loci totaling 9296bp, using sequence from Hordeum bulbosum to infer the ancestral state of mutations. The sample includes 36 accessions of cultivated barley, including 23 landraces (cultivated forms not subject to modern breeding) and 13 cultivated lines and genetic stocks compared to either 25 or 45 accessions of wild barley for the same loci. Estimates of nucleotide sequence diversity indicate that landraces retain >80% of the diversity in wild barley. The primary population structure in wild barley, which divides the species into eastern and western populations, is reflected in significant differentiation at all loci in wild accessions and at 3 of 7 loci in landraces. “Oriental” landrac...
Journal of Heredity, 2013
Ipomoea purpurea (common morning glory) is an annual vine native to Mexico that is well known for... more Ipomoea purpurea (common morning glory) is an annual vine native to Mexico that is well known for its large, showy flowers. Humans have spread morning glories worldwide, owing to the horticultural appeal of morning glory flowers. Ipomoea purpurea is an opportunistic colonizer of disturbed habitats including roadside and agricultural settings, and it is now regarded as a noxious weed in the Southeastern US. Naturalized populations in the Southeastern United States are highly polymorphic for a number of flower color morphs, unlike native Mexican populations that are typically monomorphic for the purple color morph. Although I. purpurea was introduced into the United States from Mexico, little is known about the specific geographic origins of US populations relative to the Mexican source. We use resequencing data from 11 loci and 30 I. purpurea accessions collected from the native range of the species in Central and Southern Mexico and 8 accessions from the Southeastern United States to infer likely geographic origins in Mexico. Based on genetic assignment analysis, haplotype composition, and the degree of shared polymorphism, I. purpurea samples from the Southeastern United States are genetically most similar to samples from the Valley of Mexico and Veracruz State. This supports earlier speculation that I. purpurea in the Southeastern United States was likely to have been introduced by European colonists from sources in Central Mexico.
Journal of Cellular and Molecular Medicine, 2005
With advancing age, there is a progressive reduction in cognitive function, learning and fine mot... more With advancing age, there is a progressive reduction in cognitive function, learning and fine motor control. Instead of decreased neuronal numbers, normal brain ageing is associated with a number of subtler functional, metabolic and morphological changes [1, 2]. In the cerebellum, the cellular loss with age is minimal, in both humans [3] and in rat models [4, 5]. Instead, ageing is associated with a reduction in Purkinje cell (PC) volume [3, 5], a decrease in the number of synapses in the molecular layer (ML) (predominantly between the parallel fibers and PC dendrites) [6] and a significant shortening of the length of parallel fibers [7], resulting in a retracted connectivity. Neurogenesis is also reduced with age [8]. But can be restored in the ageing brain through various methods [9], including the administration of growth factors, such as FGF-2 [10]. These data indicate that there is no intrinsic age-dependent limitation of neurogenesis and point to the possibility that during ageing the availability of growth factors is decreased.
Arthrogryposis multiplex congenita is a rare non- progressive syndrome consists of a heterogeneou... more Arthrogryposis multiplex congenita is a rare non- progressive syndrome consists of a heterogeneous group of congenital malformations of unknown etiology, multifactorial, mainly characterized by multiple joint contractures. Objective: to evaluate the importance of physiotherapy performance in patients with arthrogryposis multiplex congenita, as well as treatment methods and physical-motor and psychosocial benefits. Method: this study consists of a systematic review of articles in which search was conducted in databases like PubMed, LILACS, SciELO. The keywords used in the search were Arthrogryposis multiplex congenita and Physiotherapy, without language restriction. Results and final considerations: in total seven articles met all inclusion criteria. Only four reported a physical therapy evaluation. A varied number of intervention techniques and results were described. This systematic review demonstrated the importance of physiotherapy performance in patients with arthrogryposis mult...
Biomarker Insights, 2007
Background Pesticides are of concern in Bolivia because of increasing use. Frequent intoxications... more Background Pesticides are of concern in Bolivia because of increasing use. Frequent intoxications have been demonstrated due to use of very toxic pesticides, insufficient control of distribution and sale and little knowledge among farmers of protective measures and hygienic procedures. Method Questionnaires were applied and blood tests taken from 81 volunteers from La Paz County, of whom 48 were pesticide exposed farmers and 33 non-exposed controls. Sixty males and 21 females participated with a mean age of 37.3 years (range 17–76). Data of exposure and possible genetic damage were collected and evaluated by well known statistical methods, controlling for relevant confounders. To measure genetic damage chromosomal aberrations and the comet assay analysis were performed. Results Pesticide exposed farmers had a higher degree of genetic damage compared to the control group. The number of chromosomal aberrations increased with the intensity of pesticide exposure. Females had a lower num...
Jornal Brasileiro de Psiquiatria, 2012
OBJETIVO: Analisar o perfil de humor e fatores associados em participantes de um programa de reab... more OBJETIVO: Analisar o perfil de humor e fatores associados em participantes de um programa de reabilitação cardiopulmonar e metabólica. MÉTODO: Cento e quarenta e quatro pacientes responderam a um questionário de caracterização e à Escala de Humor de Brunel (BRUMS). O questionário de caracterização foi constituído de perguntas sobre dados pessoais e clínicos, enquanto o BRUMS avaliou o humor por meio de seis fatores: tensão, depressão, raiva, vigor, fadiga e confusão. Os dados foram tratados com estatística descritiva e inferencial (α = 0,05). RESULTADOS: De maneira geral, os pacientes com doenças cardiovasculares e metabólicas apresentaram boa saúde mental, com níveis elevados de vigor, associados à moderada tensão, depressão, raiva, fadiga e confusão. Os participantes idosos apresentaram menor tensão, raiva, fadiga e confusão do que os adultos, enquanto os participantes com dislipidemia tinham maior tensão. Aqueles com insuficiência cardíaca eram mais tensos e fadigados. Quem fuma ...
Revista Cientifica Ciencia Medica, 2014
Las infecciones nosocomiales constituyen un factor que aumenta la morbimortalidad sobre todo en e... more Las infecciones nosocomiales constituyen un factor que aumenta la morbimortalidad sobre todo en el paciente inmunosuprimido como los infantiles quemados. Es por ello que este trabajo tiene como objetivo identificar Pseudomonas aeruginosa y Staphylococcus aureus en el pabellon infantil de quemados del Hospital Viedma. El estudio es de tipo descriptivo transversal, el universo fue el pabellon infantil de quemados y la muestra los materiales y equipos que esten en frecuente contacto con los ninos quemados: Batas de visita, grifo del bano, manguera del equipo de suero, agarrador de cama, sabanas, manija de la puerta, sillon, paredes, agua de hidroterapia, tina de hidroterapia, frazadas esteriles, estantes, tina de bano, balanza, mascara de respirador, uniforme del personal de salud, manos de las enfermeras y medio ambiente. Se realizo un hisopado, se sembro en Agar Sangre y Agar Mckoney para identificar Staphylococcus aureus y Pseudomonas aeruginosa adicionado el metodo de la coagulasa en las colonias de Staphylococcus aureus; posteriormente se realizo el perfil antimicrobiano. Los resultados evidenciaron la identificacion de Staphylococcus aureus, no asi de Pseudomonas aeruginosa, en frazadas, sabanas, frazadas esteriles y manos del personal de salud. El antibiograma dio como resultado que son resistentes a la Meticilina y la colonia identificada en las frazadas esteriles tambien es resistente a Vancomicina. Se concluye que Staphylococcus aureus coloniza varios elementos del pabellon de quemados, por otro lado hay que tener en cuenta su perfil de resistencia para tratar infecciones por esta causa dentro el pabellon de quemados del Hospital Viedma
Rbm Rev Bras Med, May 1, 2013
Em pacientes com Aids a terapia antirretroviral de alta potencia (TARV) costuma causar disturbios... more Em pacientes com Aids a terapia antirretroviral de alta potencia (TARV) costuma causar disturbios nos sistemas imunologico, cardiorrespiratorio e muscular, o que pode ser minimizado pelo treinamento fisico e suplementacao com hormonio anabolizante. Objetivo: Examinar na literatura as evidencias sobre a influencia do treinamento fisico e a suplementacao com hormonio anabolizante sobre as funcoes imunologica, cardiorrespiratoria e muscular, na populacao infectada pelo virus HIV. Metodo: Revisao sistematica nas bases de dados SciELO, PUBMED, LILACS, MEDLINE, COCHRANE DATABASE, utilizando os seguintes descritores: HIV/Aids and antiretroviral treatment, exercise training, physical activity and HIV, physical activity and testosterone, testosterone and HIV, anabolic steroids, immune system, cardiorespiratory system, muscular system e seus respectivos na lingua portuguesa. Foram incluidas publicacoes desde 2000, que fossem meta-analise, ensaio clinico controlado, estudo epidemiologico do tipo coorte, estudo epidemiologico do tipo caso-controle ou revisao sistematica que avaliassem a influencia do exercicio fisico e dos hormonios anabolicos sobre as funcoes imunologica, cardiorrespiratoria e muscular. Resultados e conclusoes: Foram encontrados 14 artigos, demonstrando a melhora das funcoes cardiorrespiratoria, imunologica ou muscular, decorrente do exercicio fisico isolado, sendo que o uso exclusivo de anabolizante ou associado ao exercicio contribuiu apenas para ganho adicional de massa magra, conforme relatado em dois artigos
Cartilla pedagógica de actualidad internacional taller: un mundo más allá de nuestras fronteras. ... more Cartilla pedagógica de actualidad internacional taller: un mundo más allá de nuestras fronteras. Realizado durante el primer semestre de 2007 en la localidad de Suba / Ana Cecilia Burgos González...[et. al].
Brazilian Journal of Biomechanics Revista Brasileira De Biomecânica, Jun 25, 2013
A distrofia miotonica de Steinert (DMS) e a forma mais frequente de distrofia muscular em adultos... more A distrofia miotonica de Steinert (DMS) e a forma mais frequente de distrofia muscular em adultos, sendo o acometimento da musculatura respiratoria sua principal causa de mortalidade. Na DMS o musculo diafragma encontra-se enfraquecido em decorrencia da evolucao da doenca e os musculos esternocleidomastoideo (ECM) e trapezio superior sao ativados como forma compensatoria da respiracao. A eletromiografia (EMG) e uma ferramenta muito utilizada no diagnostico DMS, porem sua incidencia tem se mostrado variada devido ao grande numero de musculos estudados e a interpretacao dos achados diferenciados. Para permitir uma comparacao entre os estudos torna-se relevante a normalizacao dos dados. Objetivo principal: Analisar em pacientes portadores de DMS diferentes procedimentos de normalizacao da amplitude EMG dos musculos ECM e trapezio superior durante a ativacao destes na fase inspiratoria. Materiais e Metodos: Foram avaliados quatro pacientes do sexo feminino, com idade entre 18 e 44 anos atendidas em instituicao de Portadores de Distrofias Musculares Progressivas. Os dados EMG foram coletados em quatro posicionamentos diferentes para cada musculo estudado quantificados em RMS (raiz quadrada da media) e expressos em microvolts. Resultados: As CIVR (Contracao Voluntaria de Referencia) de elevacao de membros superiores ao ângulo de 60° com carga de 500gr nas maos, e elevacao do pescoco em posicao de deitada, foram aquelas que apresentaram menor variabilidade. Conclusao: a atividade respiratoria de pacientes com DMS pode ser normalizada no entanto, devem ser realizados novos estudos com maior numero de sujeitos a fim de verificar a confirmacao dos achados eletromiograficos encontrados.
Revista Brasileira de Atividade Física & Saúde, 2014
The infant juvenile obesity is currently one of the most serious public health problems of the 21... more The infant juvenile obesity is currently one of the most serious public health problems of the 21st century, being directly related to physical inactivity as a decisive factor in the worldwide epidemic. The programs of conventional workouts have become not so attractive for this particular population. Because of that the access and permanence in those structured physical activity programs for obese adolescents are difficult. This systematic review has the general goal to verify what benefits the systematic practice of hip hop dance, can promote in obese children and adolescents. The search for relevant articles was conducted in the electronic databases Medline (Pubmed), Cochrane, LILACS, SciELO from January through June 2014, being structured as [(hip hop /) and (obesity /) and (children/ adolescent), controlled and randomized trials were included regarding the systematic practice of hip hop dance as exercise and its benefits for obese children and adolescents, subjects aged 2-19, considered obese and/ or overweight. A total of 21 articles were identified in the survey. Based on the found eligible articles for the full review, only one article met all the inclusion criteria. This preliminary study is intended to broaden the context in health for adolescents and children suffering from overweight and/or obesity, recommending an intervention through dance hip hop, it would be an important modality association for possible monitoring and reducing the risk of associated comorbidities. In this field of research, there is a lack of studies of high methodological quality, which may be able to demonstrate the benefits of this type of intervention.
The American journal of otology, 2000
This study aimed to analyze the localization and distribution of vessels and of these angiogenic ... more This study aimed to analyze the localization and distribution of vessels and of these angiogenic growth factors: basic fibroblast growth factor (FGF-2), transforming growth factor-alpha (TGF-alpha), transforming growth factor-beta1 (TGF-beta1), and vascular endothelial growth factor (VEGF) in middle ear cholesteatoma in comparison with normal middle ear mucosa and auditory meatal skin. Angiogenesis is particularly important in many normal and pathologic processes, including wound healing and inflammation. Because proliferating tissues require an enhanced blood supply, angiogenesis appears to be a prerequisite for the expansion of cholesteatoma. The expression of FGF-2, TGF-alpha, TGF-beta1, and VEGF was studied by immunohistochemistry. The amount of vessels (collagen type IV staining) was determined by an automatic imaging analyzing system. The results showed an altered expression and distribution of VEGF, FGF-2, TGF-alpha, and TGF-beta1 in cholesteatoma in relation to middle ear mu...
Brazilian Journal of Physical Therapy, 2014
Background: Patients with heart failure (HF) usually develop exercise intolerance. In this contex... more Background: Patients with heart failure (HF) usually develop exercise intolerance. In this context, noninvasive ventilation (NIV) can help to increase physical performance. Objective: To undertake a systematic review and meta-analysis of randomized controlled trials that evaluated the effects of NIV on exercise tolerance in patients with HF. Method: Search Strategy: Articles were searched in the following databases: Physiotherapy Evidence Database (PEDro), Scientific Electronic Library Online (SciELO), and MEDLINE. Selection Criteria: This review included only randomized controlled trials involving patients with HF undergoing NIV, with or without other therapies, that used exercise tolerance as an outcome, verified by the distance travelled in the six-minute walk test (6MWT), VO 2 peak in the cardiopulmonary test, time spent in testing, and dyspnea. Data Collection and Analysis: The methodological quality of the studies was rated according to the PEDro scale. Data were pooled in fixed-effect meta-analysis whenever possible. Results: Four studies were selected. A meta-analysis including 18 participants showed that the use of NIV prior to the 6MWT promoted increased distance, [mean difference 65.29 m (95% CI 38.80 to 91.78)]. Conclusions: The use of NIV prior to the 6MWT in patients with HF may promote increased distance. However, the limited number of studies may have compromised a more definitive conclusion on the subject.
Revista Brasileira de Medicina do Esporte, 2012
O presente estudo teve por objetivo traduzir e validar para a língua portuguesa o Questionário Nu... more O presente estudo teve por objetivo traduzir e validar para a língua portuguesa o Questionário Nutricional Simplificado de Apetite (QNSA), verificando sua clareza e validade para participantes de programa de Reabilitação Cardiopulmonar e Metabólica (RCPM). Cento e quarenta e seis indivíduos foram entrevistados, sendo 79 homens, com média de idade de 63 anos (± 10) e 67 mulheres, com média de 66 anos (± 11). A análise descritiva foi apresentada em média, desvio padrão e frequência. A consistência interna da escala foi avaliada por meio do coeficiente alfa de Cronbach e a análise fatorial pelo método de extração de análise dos componentes principais utilizando os critérios de Kaiser (autovalores > 1). Todas as questões do instrumento apresentaram resultados positivos em relação à clareza. Os itens do questionário apresentaram carga fatorial superior a 0,40, com variação de 0,40 a 0,81. Com exceção da questão relacionada com a quantidade de refeições diárias, todas as demais apresen...
Revista Brasileira de Medicina do Esporte, 2014
INTRODUÇÃO: Os programas de reabilitação cardiopulmonar e metabólica (RCPM) vêm adotando protocol... more INTRODUÇÃO: Os programas de reabilitação cardiopulmonar e metabólica (RCPM) vêm adotando protocolos de treinamento físico que valorizam o volume e a alta intensidade, sendo possível o aparecimento da síndrome do excesso de treinamento (SET). Neste contexto, o uso sistemático de instrumentos para investigação do estado de humor pode auxiliar na detecção precoce da SET.OBJETIVO: Validar a escala de Humor de Brunel (BRUMS) em participantes de RCPM.MÉTODOS: Fizeram parte do estudo 247 indivíduos, com média de idade de 63,3±10,5 anos, participantes há pelo menos três meses de programa de RCPM. A análise descritiva foi apresentada em média, desvio padrão e distribuição de frequência. Para avaliar a validade de construto foi utilizada a análise fatorial confirmatória. Para verificar a adequação dos dados foi utilizado o índice de Kaiser-Meyer-Olkin (KMO). Para interpretação da matriz, foi aplicado o método de extração dos componentes principais, por meio do método varimax. A consistência f...
G3 (Bethesda, Md.), Jan 22, 2014
Genetic differentiation in natural populations is driven by geographic distance and by ecological... more Genetic differentiation in natural populations is driven by geographic distance and by ecological or physical features within and between natural habitats that reduce migration. The primary population structure in wild barley differentiates populations east and west of the Zagros Mountains. Genetic differentiation between eastern and western populations is uneven across the genome and is greatest on linkage groups 2H and 5H. Genetic markers in these two regions demonstrate the largest difference in frequency between the primary populations and have the highest informativeness for assignment to each population. Previous cytological and genetic studies suggest there are chromosomal structural rearrangements (inversions or translocations) in these genomic regions. Environmental association analyses identified an association with both temperature and precipitation variables on 2H and with precipitation variables on 5H.
The Plant Journal, 2013
Advanced resources for genome-assisted research in barley (Hordeum vulgare) including a whole-gen... more Advanced resources for genome-assisted research in barley (Hordeum vulgare) including a whole-genome shotgun assembly and an integrated physical map have recently become available. These have made possible studies that aim to assess genetic diversity or to isolate single genes by whole-genome resequencing and in silico variant detection. However such an approach remains expensive given the 5 Gb size of the barley genome. Targeted sequencing of the mRNA-coding exome reduces barley genomic complexity more than 50-fold, thus dramatically reducing this heavy sequencing and analysis load. We have developed and employed an in-solution hybridization-based sequence capture platform to selectively enrich for a 61.6 megabase coding sequence target that includes predicted genes from the genome assembly of the cultivar Morex as well as publicly available full-length cDNAs and de novo assembled RNA-Seq consensus sequence contigs. The platform provides a highly specific capture with substantial and reproducible enrichment of targeted exons, both for cultivated barley and related species. We show that this exome capture platform provides a clear path towards a broader and deeper understanding of the natural variation residing in the mRNA-coding part of the barley genome and will thus constitute a valuable resource for applications such as mapping-by-sequencing and genetic diversity analyzes.
Journal of Heredity, 2012
The common morning glory (Ipomoea purpurea) is an annual vine native to Central and Southern Mexi... more The common morning glory (Ipomoea purpurea) is an annual vine native to Central and Southern Mexico. The genetics of flower color polymorphisms and interactions with the biotic environment have been extensively studied in I. purpurea and in its sister species I. nil. In this study, we examine nucleotide sequence polymorphism in 11 loci, 9 of which are known to participate in a pathway that produces floral pigments. A sample of 30 I. purpurea accessions from the native range of Central and Southern Mexico comprise the data, along with one accession from each of the two sister species I. alba and I. nil. We observe moderate levels of nucleotide sequence polymorphism of ~1%. The ratio of recombination to mutation parameter estimates (ρ/θ) of ~2.5 appears consistent with a mixed-mating system. Ipomoea resequencing data from these genic regions are noteworthy in providing a good fit to the standard neutral model of molecular evolution. The derived silent site frequency spectrum is very close to that predicted by coalescent simulations of a drift-mutation process, and Tajima's D values are not significantly different from expectations under neutrality.
Journal of Heredity, 2013
The levels of diversity and extent of linkage disequilibrium in cultivated species are largely de... more The levels of diversity and extent of linkage disequilibrium in cultivated species are largely determined by diversity in their wild progenitors. We report a comparison of nucleotide sequence diversity in wild and cultivated barley (Hordeum vulgare ssp. spontaneum and ssp. vulgare) at 7 nuclear loci totaling 9296bp, using sequence from Hordeum bulbosum to infer the ancestral state of mutations. The sample includes 36 accessions of cultivated barley, including 23 landraces (cultivated forms not subject to modern breeding) and 13 cultivated lines and genetic stocks compared to either 25 or 45 accessions of wild barley for the same loci. Estimates of nucleotide sequence diversity indicate that landraces retain >80% of the diversity in wild barley. The primary population structure in wild barley, which divides the species into eastern and western populations, is reflected in significant differentiation at all loci in wild accessions and at 3 of 7 loci in landraces. “Oriental” landrac...
Journal of Heredity, 2013
Ipomoea purpurea (common morning glory) is an annual vine native to Mexico that is well known for... more Ipomoea purpurea (common morning glory) is an annual vine native to Mexico that is well known for its large, showy flowers. Humans have spread morning glories worldwide, owing to the horticultural appeal of morning glory flowers. Ipomoea purpurea is an opportunistic colonizer of disturbed habitats including roadside and agricultural settings, and it is now regarded as a noxious weed in the Southeastern US. Naturalized populations in the Southeastern United States are highly polymorphic for a number of flower color morphs, unlike native Mexican populations that are typically monomorphic for the purple color morph. Although I. purpurea was introduced into the United States from Mexico, little is known about the specific geographic origins of US populations relative to the Mexican source. We use resequencing data from 11 loci and 30 I. purpurea accessions collected from the native range of the species in Central and Southern Mexico and 8 accessions from the Southeastern United States to infer likely geographic origins in Mexico. Based on genetic assignment analysis, haplotype composition, and the degree of shared polymorphism, I. purpurea samples from the Southeastern United States are genetically most similar to samples from the Valley of Mexico and Veracruz State. This supports earlier speculation that I. purpurea in the Southeastern United States was likely to have been introduced by European colonists from sources in Central Mexico.
Journal of Cellular and Molecular Medicine, 2005
With advancing age, there is a progressive reduction in cognitive function, learning and fine mot... more With advancing age, there is a progressive reduction in cognitive function, learning and fine motor control. Instead of decreased neuronal numbers, normal brain ageing is associated with a number of subtler functional, metabolic and morphological changes [1, 2]. In the cerebellum, the cellular loss with age is minimal, in both humans [3] and in rat models [4, 5]. Instead, ageing is associated with a reduction in Purkinje cell (PC) volume [3, 5], a decrease in the number of synapses in the molecular layer (ML) (predominantly between the parallel fibers and PC dendrites) [6] and a significant shortening of the length of parallel fibers [7], resulting in a retracted connectivity. Neurogenesis is also reduced with age [8]. But can be restored in the ageing brain through various methods [9], including the administration of growth factors, such as FGF-2 [10]. These data indicate that there is no intrinsic age-dependent limitation of neurogenesis and point to the possibility that during ageing the availability of growth factors is decreased.