Andre Renard - Academia.edu (original) (raw)

Papers by Andre Renard

The EMBO Journal

Prolactin (PRL) and growth hormone (GH) genes derive from a common ancestor and still share some ... more Prolactin (PRL) and growth hormone (GH) genes derive from a common ancestor and still share some sequence homologies. Their expression in the pituitary gland is regulated in opposite directions by most of the many hormones acting on them. This provides an interesting system to study sequences involved in gene expression. Using a human PRL cDNA clone as a probe, we screened a human genomic DNA library in X phage and isolated a single recombinant comprising the whole hPRL gene. It was characterized by restriction endonuclease mapping and cDNA hybridization, by DNA heteroduplex analysis and by nucleotide sequencing. The hPRL gene is present as a single copy per haploid genome, is -10 kb long and contains four introns, three of which interrupt the coding sequence at the same locations as in the known GH and PRL genes. The origin of transcription was determined by Si mapping on prolactinoma mRNAs. The search for direct and inverted repeats, as well as dyad symmetries was carried out in the 900-bp sequenced in the 5'-flanking region. Sequence homologies between hPRL, hGH and rPRL were derived from computer drawn matrices for these upstream regions.

Annales de recherches vétérinaires. Annals of veterinary research

Bovine viral diarrhea virus (BVDV) genomic RNA was identified as a 12.5-kb single-stranded RNA mo... more Bovine viral diarrhea virus (BVDV) genomic RNA was identified as a 12.5-kb single-stranded RNA molecule in both infected bovine embryonic kidney cells (BEK-1) and partially purified virions. BVD virion RNA was partially purified and used as a template for cDNA synthesis. BVDV-specific cDNA sequences were molecularly cloned and shown to hybridize to infected cell RNA but not to uninfected cell RNA or DNA. A single RNA species of 12.5 kb, representing the viral RNA genome, was detected in infected cells. A preliminary map of the BVDV specific cDNA clones was constructed and five major, nonoverlapping families were observed, accounting for approximately one-half of the viral genome.

Archives of virology. Supplementum

European journal of biochemistry / FEBS, Jan 15, 1983

Cell nuclei prepared from rat liver were alkylated in vitro with ethylnitrosourea; the nuclear DN... more Cell nuclei prepared from rat liver were alkylated in vitro with ethylnitrosourea; the nuclear DNA was found to lose O6-ethylguanine and 7-ethylguanine during a subsequent incubation at 37 degrees C. The rate of O6-ethylguanine loss is comparable to that observed in vivo, indicating that no cytoplasmic component is needed for the repair; no free O6-ethylguanine was found in the incubation medium of the ethylated nuclei. The rate of 7-ethylguanine loss is higher than the spontaneous depurination in vitro and an amount of free 7-ethylguanine equivalent to that lost by the nuclear DNA was found in the incubation medium; these results suggest that this DNA lesion is excised by a DNA glycosylase. The proteins of the chromatin prepared from the isolated nuclei induced the disappearance of O6-ethylguanine from an added ethylated DNA. No free O6-ethylguanine was released indicating that the repair is not catalyzed by a DNA glycosylase; no oligonucleotides enriched in O6-ethylguanine were re...

European Journal of Biochemistry, 1983

Chromatin proteins from rat liver contain a repair activity that removes 06-ethylguanine from eth... more Chromatin proteins from rat liver contain a repair activity that removes 06-ethylguanine from ethylnitrosoureatreated DNA. This activity does not depend on divalent cations and works in the presence of EDTA, but does depend on the presence of free thiol groups. Thus, it is destroyed by N-ethylmaleimide and is protected by dithiothreitol. The repair activity on single-stranded DNA is only 20 % of what it is on double-stranded DNA; its half-life at 35 "C is 55 min, but DNA, ethylated or not, affords some protection.

$Research paper thumbnail of Repair of O6-ethylguanine in DNA by a chromatin fraction from rat liver: transfer of the ethyl group to an acceptor protein$

Proceedings of the National Academy of Sciences, 1981

Journal of Theoretical Biology, 1983

Gene, 1992

We describe a modification of the pAR3040 vector which results in its efficient stabilization dur... more We describe a modification of the pAR3040 vector which results in its efficient stabilization during cell division. The parB locus of the plasmid Rl was introduced into the plasmid, pAR3040, to construct the pARHS vectors. These vectors are stable for at least 60 cell generations, even in the absence of selection by an antibiotic present in the culture media, both with or without IPTG induction.

Gene, 1990

To allow subsequent genetically mediated fusion of foreign antigens to cholera toxin B subunit (C... more To allow subsequent genetically mediated fusion of foreign antigens to cholera toxin B subunit (CTB), two mutated CTB encoding genes (ctxB) were constructed and overexpressed in Escherichia coli. The signal peptide coding sequence was deleted and restriction sites were created at both ends of the modified sequence. Both synthesized CTBs contain additional amino acid(s) at the N terminus (one and three). They were purified as insoluble products and refolded into the natural pentameric CTB structure by a denaturation-renaturation cycle. After renaturation, both recombinant proteins recovered CTB antigenicity and the ability to bind to GM1 gangliosides, as shown by in vitro analysis. Preliminary data indicated that both properties were unaltered by fusion of a foreign peptide to the mutated CTBs.

Biochimie, 1982

Disappearance of 7-ethylguanine and O6-ethylguanine lesions from nuclear DNA is observed on incub... more Disappearance of 7-ethylguanine and O6-ethylguanine lesions from nuclear DNA is observed on incubation of isolated rat liver nuclei previously treated with ethylnitrosourea. Free 7-ethylguanine but no free O6-ethylguanine is released in the medium. Incubation of methylated or ethylated DNA with chromatin proteins leads to the disappearance of O6-methylguanine or O6-ethylguanine from DNA; the methyl group or the ethyl group is transferred to proteins where it is accepted by cystein residues. Repair of O6-methylguanine and repair of O6-ethylguanine lesions in DNA consume a common reactant which is likely the acceptor protein. The repair does not seem however to be a simple bimolecular reaction between the O6-alkylguanine and the cystein of the acceptor protein.