AbDul NaSir - Academia.edu (original) (raw)

Papers by AbDul NaSir

Environmental Science & Technology, 2019

Total number of pages of SI are 17 (S1-S19) S-3 Experimental Method Chemical Reagents Orange II (... more Total number of pages of SI are 17 (S1-S19) S-3 Experimental Method Chemical Reagents Orange II (OR) used in this study was purchased from Sandal dyes industries. Wastewater was acquired from three different textile industries (named as Nimra textile industry effluent (NIE1), Bismilla textile industry effluent (BIE2), and Chawla textile industry effluent (CIE3)) for the evaluation of their physico-chemical properties before and after adsorption and degradation of dyes. Poly vinyl alcohol (PVA), sodium alginate (Na-alginate), sodium hydroxide (NaOH), hydrochloric acid (HCl) were purchased from Sigma Aldrich and used without any further purification. Collection and Grinding of Eleocharis Native and clean pieces of Eleocharis were collected from the Pagel Industries in relatively dried and substantial form. Dried biomass was ground to achieve robust and uniform particle size (250 µm) of Eleocharis, advance Laboratory Mill 120 (16800 rpm@ 105558 rad min-1 , Perten Instruments, Australia) was used for grinding. Culture and Extraction of Manganese Peroxidase Enzyme (MnPE) MnPE was extracted from the culture of Ganoderma lucidum IBL-05 (hereafter refer as lucidum) fungal strain, briefly 10 mL of lucidum containing 1 × 10 5 spores/mL provided with moistened (66 % w/w of Kirk's basal nutrient medium) wheat bran (5 g) was fermented at pH of 4.5 and 30 °C for 6 days. Later on, fermented mixture was diluted with 100 mL water, shaken and centrifuged. Clear supernatant was taken and saturated with slow addition of ammonium sulphate followed by overnight precipitation at 4 °C. Precipitates were collected by centrifugation whereas saturation step with supernatant was repeated thrice. The obtained pallets were dissolved in sodium malonate buffer (pH 4.5, 50 mM), dialyzed, purified through ion exchanged chromatography, concentrated and stored at-20 °C for further use.

BMC Infectious Diseases, 2012

Background: Few data are available in Afghanistan to shape national military force health practic... more Background: Few data are available in Afghanistan to shape national military force health practices, particularly with regard to sexually-transmitted infections (STIs). We measured prevalence and correlates of HIV, syphilis, herpes simplex 2 virus (HSV-2), and hepatitis C virus (HCV) among Afghan National Army (ANA) recruits. Methods: A cross-sectional sample of male ANA recruits aged 18-35 years were randomly selected at the Kabul Military Training Center between February 2010 and January 2011. Participants completed an interviewer-administered questionnaire and serum-based rapid testing for syphilis and hepatitis C virus antibody on-site; HIV and HSV-2 screening, and confirmatory testing were performed off-site. Prevalence of each infection was calculated and logistic regression analysis performed to identify correlates. Results: Of 5313 recruits approached, 4750 consented to participation. Participants had a mean age of 21.8 years (SD±3.8), 65.5% had lived outside Afghanistan, and 44.3% had no formal education. Few reported prior marijuana (16.3%), alcohol (5.3%), or opiate (3.4%) use. Of sexually active recruits (58.7%, N = 2786), 21.3% reported paying women for sex and 21.3% reported sex with males. Prevalence of HIV (0.063%, 95% CI: 0.013-0.19), syphilis (0.65%, 95% CI: 0.44-0.93), and HCV (0.82%, 95% CI: 0.58-1.12) were quite low. Prevalence of HSV-2 was 3.03% (95% CI: 2.56-3.57), which was independently associated with age (Adjusted Odds Ratio (AOR) = 1.04, 95% CI: 1.00-1.09) and having a television (socioeconomic marker) (AOR = 1.46, 95% CI: 1.03-2.05). Conclusion: Though prevalence of HIV, HCV, syphilis, and HSV-2 was low, sexual risk behaviors and intoxicant use were present among a substantial minority, indicating need for prevention programming. Formative work is needed to determine a culturally appropriate approach for prevention programming to reduce STI risk among Afghan National Army troops.

A total of 500 serum samples collected from cattle and buffaloes from various livestock farms in ... more A total of 500 serum samples collected from cattle and buffaloes from various livestock farms in Punjab were analysed for brucella antigens. Out of these 500 sera, Rose Bengal Plate Test (RBPT) recorded 79(15.8%) positive. These RBPT positive samples were subjected to routine serum agglutination (SAT) and modified SAT (EDTA added) tests. The SAT showed 18(22.7%) positive, 42(53.1%) doubtful and

Environmental Science & Technology, 2019

Total number of pages of SI are 17 (S1-S19) S-3 Experimental Method Chemical Reagents Orange II (... more Total number of pages of SI are 17 (S1-S19) S-3 Experimental Method Chemical Reagents Orange II (OR) used in this study was purchased from Sandal dyes industries. Wastewater was acquired from three different textile industries (named as Nimra textile industry effluent (NIE1), Bismilla textile industry effluent (BIE2), and Chawla textile industry effluent (CIE3)) for the evaluation of their physico-chemical properties before and after adsorption and degradation of dyes. Poly vinyl alcohol (PVA), sodium alginate (Na-alginate), sodium hydroxide (NaOH), hydrochloric acid (HCl) were purchased from Sigma Aldrich and used without any further purification. Collection and Grinding of Eleocharis Native and clean pieces of Eleocharis were collected from the Pagel Industries in relatively dried and substantial form. Dried biomass was ground to achieve robust and uniform particle size (250 µm) of Eleocharis, advance Laboratory Mill 120 (16800 rpm@ 105558 rad min-1 , Perten Instruments, Australia) was used for grinding. Culture and Extraction of Manganese Peroxidase Enzyme (MnPE) MnPE was extracted from the culture of Ganoderma lucidum IBL-05 (hereafter refer as lucidum) fungal strain, briefly 10 mL of lucidum containing 1 × 10 5 spores/mL provided with moistened (66 % w/w of Kirk's basal nutrient medium) wheat bran (5 g) was fermented at pH of 4.5 and 30 °C for 6 days. Later on, fermented mixture was diluted with 100 mL water, shaken and centrifuged. Clear supernatant was taken and saturated with slow addition of ammonium sulphate followed by overnight precipitation at 4 °C. Precipitates were collected by centrifugation whereas saturation step with supernatant was repeated thrice. The obtained pallets were dissolved in sodium malonate buffer (pH 4.5, 50 mM), dialyzed, purified through ion exchanged chromatography, concentrated and stored at-20 °C for further use.

BMC Infectious Diseases, 2012

Background: Few data are available in Afghanistan to shape national military force health practic... more Background: Few data are available in Afghanistan to shape national military force health practices, particularly with regard to sexually-transmitted infections (STIs). We measured prevalence and correlates of HIV, syphilis, herpes simplex 2 virus (HSV-2), and hepatitis C virus (HCV) among Afghan National Army (ANA) recruits. Methods: A cross-sectional sample of male ANA recruits aged 18-35 years were randomly selected at the Kabul Military Training Center between February 2010 and January 2011. Participants completed an interviewer-administered questionnaire and serum-based rapid testing for syphilis and hepatitis C virus antibody on-site; HIV and HSV-2 screening, and confirmatory testing were performed off-site. Prevalence of each infection was calculated and logistic regression analysis performed to identify correlates. Results: Of 5313 recruits approached, 4750 consented to participation. Participants had a mean age of 21.8 years (SD±3.8), 65.5% had lived outside Afghanistan, and 44.3% had no formal education. Few reported prior marijuana (16.3%), alcohol (5.3%), or opiate (3.4%) use. Of sexually active recruits (58.7%, N = 2786), 21.3% reported paying women for sex and 21.3% reported sex with males. Prevalence of HIV (0.063%, 95% CI: 0.013-0.19), syphilis (0.65%, 95% CI: 0.44-0.93), and HCV (0.82%, 95% CI: 0.58-1.12) were quite low. Prevalence of HSV-2 was 3.03% (95% CI: 2.56-3.57), which was independently associated with age (Adjusted Odds Ratio (AOR) = 1.04, 95% CI: 1.00-1.09) and having a television (socioeconomic marker) (AOR = 1.46, 95% CI: 1.03-2.05). Conclusion: Though prevalence of HIV, HCV, syphilis, and HSV-2 was low, sexual risk behaviors and intoxicant use were present among a substantial minority, indicating need for prevention programming. Formative work is needed to determine a culturally appropriate approach for prevention programming to reduce STI risk among Afghan National Army troops.

A total of 500 serum samples collected from cattle and buffaloes from various livestock farms in ... more A total of 500 serum samples collected from cattle and buffaloes from various livestock farms in Punjab were analysed for brucella antigens. Out of these 500 sera, Rose Bengal Plate Test (RBPT) recorded 79(15.8%) positive. These RBPT positive samples were subjected to routine serum agglutination (SAT) and modified SAT (EDTA added) tests. The SAT showed 18(22.7%) positive, 42(53.1%) doubtful and