Abdelkhalek Oussama - Academia.edu (original) (raw)
Papers by Abdelkhalek Oussama
Background: The use of herbal medicines (medicinal plants or phytotherapy) has recently gained po... more Background: The use of herbal medicines (medicinal plants or phytotherapy) has recently gained popularity in Europe and the United States. Nevertheless the exact mechanism of the preventive effects of these products is still far to be clearly established, being its knowledge necessary to successfully apply these therapies to avoid stone formation. Methods: The effect of oral lemon juice administration on calcium oxalate urolithiasis was studied in male Wistar rats. Rats were rendered nephrolithic by providing drinking water containing 0.75% ethylene glycol [v/v] (EG) and 2% ammonium chloride [w/v] (AC) for 10 days. In addition to EG/ AC treatment, three groups of rats were also gavage-administered solutions containing 100%, 75% or 50% lemon juice [v/v] (6 µl solution/g body weight). Positive control rats were treated with EG/ AC but not lemon juice. Negative control rats were provided with normal drinking water, and were administered normal water by gavage. Each group contained 6 rats. After 10 days, serum samples were collected for analysis, the left kidney was removed and assessed for calcium levels using flame spectroscopy, and the right kidney was sectioned for histopathological analysis using light microscopy. Results: Analysis showed that the rats treated with EG/AC alone had higher amounts of calcium in the kidneys compared to negative control rats. This EG/AC-induced increase in kidney calcium levels was inhibited by the administration of lemon juice. Histology showed that rats treated with EG/AC alone had large deposits of calcium oxalate crystals in all parts of the kidney, and that such deposits were not present in rats also treated with either 100% or 75% lemon juice. Conclusion: These data suggest that lemon juice has a protective activity against urolithiasis.
<b>Copyright information:</b>Taken from "Lemon juice has protective activity in ... more <b>Copyright information:</b>Taken from "Lemon juice has protective activity in a rat urolithiasis model"http://www.biomedcentral.com/1471-2490/7/18BMC Urology 2007;7():18-18.Published online 5 Oct 2007PMCID:PMC2194764. Sections were viewed using a BX41 optical microscope and polarized light. a: Tissue from negative control rats, b: Tissue from rats treated with ethylene glycol (EG), ammonium chloride (AC) and 100% lemon juice, c: Tissue from rats treated with EG, AC and 75% lemon juice, d: Tissue from rats treated with EG, AC and 50% lemon juice, e: Tissue from rats treated with EG and AC only (positive control). Crystalline formations in the renal papilla are indicated by arrows. Magnification ×100.
<b>Copyright information:</b>Taken from "Lemon juice has protective activity in ... more <b>Copyright information:</b>Taken from "Lemon juice has protective activity in a rat urolithiasis model"http://www.biomedcentral.com/1471-2490/7/18BMC Urology 2007;7():18-18.Published online 5 Oct 2007PMCID:PMC2194764. Sections were viewed using a BX41 optical microscope and polarized light. a: Tissue from negative control rats, b: Tissue from rats treated with ethylene glycol (EG), ammonium chloride (AC) and 100% lemon juice, c: Tissue from rats treated with EG, AC and 75% lemon juice, d: Tissue from rats treated with EG, AC and 50% lemon juice, e: Tissue from rats treated with EG and AC only (positive control). Crystalline formations in the renal parenchyma are indicated by arrows. Magnification ×100.
<b>Copyright information:</b>Taken from "Lemon juice has protective activity in ... more <b>Copyright information:</b>Taken from "Lemon juice has protective activity in a rat urolithiasis model"http://www.biomedcentral.com/1471-2490/7/18BMC Urology 2007;7():18-18.Published online 5 Oct 2007PMCID:PMC2194764. Values represent mean ± SD (μg/g) for six animals in each group. Values are significantly different from the negative control group: *p < 0.05, **p < 0.01, ***p < 0.001. Values are significantly different from the positive control group: * p < 0.05, **p < 0.01, ***p < 0.001.
The Egyptian Journal of Chemistry, 2021
This study aims to create a model of oleaster oil simply and reliably to detect adulteration, whi... more This study aims to create a model of oleaster oil simply and reliably to detect adulteration, which presents a large danger that attacks the food sector and human health. For this reason, a study to detect the falsification of oleaster oil was carried out by Fourier-Transform Infrared Spectroscopy FTIR and chemometric method. The experimental samples are shared into two sets, 32 Training set, 8 Test set (4 calibration samples opposite one for validation), and a falsification interval of 1.5-40%. The treatment of infrared spectral results has been done by chemometrics techniques utilizing Partial Least Squares regression or Projection to Latent Structures (PLSR) and Principal Component Regression (PCR). The results show that the perfect falsification model of oleaster oil by olive-oil and soybean oil is illustrated in the spectral region 3050-2700 cm, with R of 0.999 from PLSR and PCR to soybean-oil, concerning olive-oil shows also the better results for the PLSR technical with R of ...
Hirri A., De Luca M., Ioele G., Balouki A., Bassbasi M., Kzaiber F., Oussama A., Ragno G. (2015):... more Hirri A., De Luca M., Ioele G., Balouki A., Bassbasi M., Kzaiber F., Oussama A., Ragno G. (2015): Chemometric classification of citrus juices of Moroccan cultivars by infrared spectroscopy. Czech J. Food Sci., 33: 137–142. Fourier transform – infrared (FTIR) spectroscopy in connection with chemometric methodologies was used as a fast and direct analytical approach to classify citrus cultivars by the measurements on their juice. Modern multivariate analysis responds to the current needs for economic, simple, and fast methods able to classify new unknown samples with great accuracy. A set of 135 samples of citrus juice, representative of three cultivars (Hamlin, Muska, and Valencia), all picked in the same geographical area of Morocco, were analysed. Chemometric discrimination of the juice samples was achieved by principal component analysis (PCA) performed on the FTIR spectral data from the juice samples, showing an explained variance of 97.84% by considering only 2 PCs. A fully corr...
Journal of Sensory Studies, 2021
International Journal of Innovation and Applied Studies, 2013
The present work concerns the study of the nutritional quality of the raw milk, on samples result... more The present work concerns the study of the nutritional quality of the raw milk, on samples resulting from centers of collection of the milk (cooperatives) and from farms, from the region of Tadla (Kssiba and Fkih ben Saleh) and the region of Kelaa. The investigations concerned two periods of year 2011 (Period I: February-March-April and Period II: September-October-November). The analysis results show that for the three nutritional parameters, protein content (TP), fat (MG) and defatted dry matter (ESD), there are variations between samples. Assessing the nutritional quality of milk is required to highlight the variability in the nutritional quality of milk production, evaluating the quality of milk produced in those regions and determine the factors influential to improve production and profitability. This quality is demonstrated through the determination of three main parameters of milk constituents, namely fat (MG) Protein content (TP) and nonfat dry (ESD). Milk quality plays an ...
Ensure the quality and authenticity of the products has been a concern of several country. The go... more Ensure the quality and authenticity of the products has been a concern of several country. The goal of this study was to attempt classification of moroccan olives according to their botanical and geographical origin based on the endocarp analysis by Attenuated Total Reflectance-Fourier Transform InfraRed (ATR-FTIR) spectroscopy and find the markers of their authenticity. This study was focused on the olive samples of two varietie (Picholine Marocaine and Arbequina), picked from three farms belong to the three zones: named OuledMbarek, ouledyussuf and Sidijaber, in the Moroccan region of Beni Mellal. The classification models were developed by principal component analysis (PCA) and support vector machines (SVM). On the basis of a PCA, six distinct clusters were recognized. The SVM procedure was then elaborated. The model resulted able to separate the six classes and classify new samples into the appropriate defined classes with a percentage prediction of 95%. This Result show the cap...
Fourier transform–infrared (FTIR) spectroscopy in connection with chemometric methodologies was u... more Fourier transform–infrared (FTIR) spectroscopy in connection with chemometric methodologies was used to develop a fast and direct analytical approach classification model for determining the geographical origins of Navel citrus by the measurements on their juice. Modern multivariate analysis responds to the current needs for economic, simple, and fast methods able to classify new unknown samples with great accuracy. A set of 46 samples of citrus juice, representative of three geographical origins of Morrocco (Beni Mellal, Agadir, and Berkan) were analysed. Chemometric discrimination of the juice samples was achieved by principal component analysis (PCA) performed on the FTIR spectral data from the juice samples, showing an explained variance of 99% by considering only 2 PCs. A fully correct classification of the three geographical origins was then obtained by using Partial Least Square-Discriminant Analysis (PLS-DA) modeling procedure.
Olive oil is a natural product that is used in various fields, such as medicine, cosmetics and nu... more Olive oil is a natural product that is used in various fields, such as medicine, cosmetics and nutrition. It is a food product that is characterized by the multitude of its properties and its economic value. For this reason, it has been adulterated by other vegetable oils which are cheaper and whose properties are similar to those of olive oil and for more the profit. In literature study, there are a lot of works about this oil for determinate and evaluate this authenticity by different ways and techniques. FRIR coupled with Chemometrics has many special advantages because it is having an important characteristic about your manipulation and results. The aim for this review is to evaluate the capacity and the reliability for each method used in the examiner quality of this oil, and to determinate its advantages and disadvantages.
Korean Journal for Food Science of Animal Resources, 2011
Annales d'urologie, 2000
Calculi from 45 Moroccan children aged between 2 and 15 years underwent morphological and infrare... more Calculi from 45 Moroccan children aged between 2 and 15 years underwent morphological and infrared spectrometric analysis. The stones were three times more frequent in males than females (M/F = 3.09). Whewellite was the main component in 51.1% of cases and in 44.4% of stone nuclei, wheddellite in 8.9% of stones and nuclei, carbapatite in 6.7% of stones and 8.9% of nuclei, struvite in 15.6% of stones and 11.1% of nuclei. Ammonium hydrogen urate and uric acid were predominant respectively in 8.9% and 6.7% of stones and in 15.6% and 11.1% of nuclei. In addition to whewellite, struvite and ammonium hydrogen urate were the main components of bladder stones from both sexes. With respect to their calculi, whewellite was present in 84.4% of cases and wheddellite in 26.7%. Purines were present in 46% of calculi, especially as ammonium urate (28.9%) and uric acid (15.6%). Calcium phosphates as the main components were infrequent. In contrast, they were frequently identified in urinary calculi...
Annales d'urologie, 2000
Urolithiasis is a frequent disorder that is characterized by its recurrence following treatment a... more Urolithiasis is a frequent disorder that is characterized by its recurrence following treatment and which can affect between 3-20% of the population, with an incidence that differs from country to country. The aim of the present study was to determine the composition of the calculi, and the remain characteristics of this pathology in Morocco. A series of 80 calculi was therefore analyzed by Fourier transform infrared spectroscopy. The findings showed that calcium monohydrate oxalate was the main lithiasic component, indicating that hyperoxaluria plays a major role in the formation of the calculi. The component identified were as follows: calcium oxalate (58.75%), calcium phosphate and magnesium phosphate (17.5%), uric acid (15%), and urate (8.75%). In 91.25% of cases, the calculi were of mixed composition. Regular patient follow-up is advocated and subjects should be informed of the risk factor involved, as urolithiasic recurrence was observed in 10% of the cases in this series.
Food Chemistry, 2011
Fourier transform infrared (FTIR) spectra were employed for differentiation and classification of... more Fourier transform infrared (FTIR) spectra were employed for differentiation and classification of olive oils from several producing regions of Morocco. A preliminary treatment of the FTIR data was done by a derivative elaboration based on the Savitzky–Golay algorithm to reduce the noise and extract a largest number of analytical information from the spectra. A multivariate statistical procedure based on cluster
Biopolymers and Cell, 1998
Институт молекулярной биологии и генетики НАН Украины 252143, Киев, ул. Академика Заболотного, 15... more Институт молекулярной биологии и генетики НАН Украины 252143, Киев, ул. Академика Заболотного, 150 Проведено сравнение физико-химических свойств тирозил-тРНК синтетаз (КФ 6.1.1.1) из Т. thermophilus и Е. coli Определены величины констант Михаэлиса для тРНК Туг из мезофила и термофила, L-тирозина и АТР в реащии аминоацилирования. тирозил-тРНК синтетазой из Т. thermophilus при 37, 65 и 75 °С, оптимальные концентрации КС1 в реакциях перекрестного аминоацилирования. Осуществлен сравнительньиї анализ аминокислотного состава термофильной и мезофильной тирозил-тРНК синтетаз. Получены температурные зависимости скоростей реакций пирофосфатного обмена и аминоацилирования тРНК, температурные зависимости термостабильности обоих ферментов.
IOSR Journal of Environmental Science, Toxicology and Food Technology, 2016
Recently, sesame oil is highly appreciated by consumers due to its sensory qualities, antioxidant... more Recently, sesame oil is highly appreciated by consumers due to its sensory qualities, antioxidants activity and nutritional benefits, despite its high price. Also, the detection and quantification of adulteration both are problem with increasing importance in the consumer's health and food industry. The goal of this study was to use Fourier transform mid-infrared (FT-MIR) spectroscopy coupled with chemometrics for the quantification of the adulteration of sesame oil. Adulteration with sunflower oil, soybean oil or colza oil is one of the most difficult to detect due to the similar composition of them and sesame oil. Adulterations of sesame oil with different percentages of sunflower oil, soybean oil and colza oil were measured using ATR-FTIR spectroscopy. Spectral and reference data were firstly analyzed by principal component analysis. Partial least square regression (PLSR) was used to establish calibration models. Excellent correlations between ATR-FTIR analysis and studied edible oils concentration was obtained for sunflower oil (R 2 v = 0.997), soybean oil (R 2 v = 0.993) and colza oil (R 2 v = 0.994). PLS models demonstrated the feasibility of quantifiing successfully adulterations of sesame oil in the 0-35 % weight ratio range of edible oils with a rapid and inexpensive tool without the need for sample preparation.
The Egyptian Journal of Chemistry, 2021
The main objective of this study is the use of Fourier transform mid-infrared (FTIR) spectroscopy... more The main objective of this study is the use of Fourier transform mid-infrared (FTIR) spectroscopy for discrimination of samples of Argan kernels from three Moroccan regions. The content of Argan oil extracted from the kernel depends on the plant genotype and on the environmental conditions. The origin of argan kernel samples could be distinguished combining the IR spectra measurement and chemometric tools for discriminant analysis. A multivariate analysis procedure based on the combined use of Hierarchical Cluster Analysis (HCA) and Partial Least Squares-Discriminant Analysis (PLS-DA) was tested for this purpose. HCA showed three distinct clusters related to the Moroccan regions and suggested the usefulness of IR spectroscopy to distinguish among kernel origins. Afterwards, PLS-DA was used for the discrimination and classification of the origin of the various argan kernels and good classification models and validation results were obtained. For 9 new unknown samples we attribuate 10...
FT-IR coupled to chemometrics techniques was used to investigate the quality control of engine lu... more FT-IR coupled to chemometrics techniques was used to investigate the quality control of engine lubricating oils SAE 30 of gear and machines in industries. The results showed that Partial Least Squares Discriminate Analysis (PLS2-DA) gave good classification results with 100% correct class prediction. The proposed procedure is fast, non-destructive, simple and easy to operate. This developed method is highly recommended for quick monitoring of the oil’s quality, and it is another application of FTIR-chemometrics method for quality control of lubricating oils.
Background: The use of herbal medicines (medicinal plants or phytotherapy) has recently gained po... more Background: The use of herbal medicines (medicinal plants or phytotherapy) has recently gained popularity in Europe and the United States. Nevertheless the exact mechanism of the preventive effects of these products is still far to be clearly established, being its knowledge necessary to successfully apply these therapies to avoid stone formation. Methods: The effect of oral lemon juice administration on calcium oxalate urolithiasis was studied in male Wistar rats. Rats were rendered nephrolithic by providing drinking water containing 0.75% ethylene glycol [v/v] (EG) and 2% ammonium chloride [w/v] (AC) for 10 days. In addition to EG/ AC treatment, three groups of rats were also gavage-administered solutions containing 100%, 75% or 50% lemon juice [v/v] (6 µl solution/g body weight). Positive control rats were treated with EG/ AC but not lemon juice. Negative control rats were provided with normal drinking water, and were administered normal water by gavage. Each group contained 6 rats. After 10 days, serum samples were collected for analysis, the left kidney was removed and assessed for calcium levels using flame spectroscopy, and the right kidney was sectioned for histopathological analysis using light microscopy. Results: Analysis showed that the rats treated with EG/AC alone had higher amounts of calcium in the kidneys compared to negative control rats. This EG/AC-induced increase in kidney calcium levels was inhibited by the administration of lemon juice. Histology showed that rats treated with EG/AC alone had large deposits of calcium oxalate crystals in all parts of the kidney, and that such deposits were not present in rats also treated with either 100% or 75% lemon juice. Conclusion: These data suggest that lemon juice has a protective activity against urolithiasis.
<b>Copyright information:</b>Taken from "Lemon juice has protective activity in ... more <b>Copyright information:</b>Taken from "Lemon juice has protective activity in a rat urolithiasis model"http://www.biomedcentral.com/1471-2490/7/18BMC Urology 2007;7():18-18.Published online 5 Oct 2007PMCID:PMC2194764. Sections were viewed using a BX41 optical microscope and polarized light. a: Tissue from negative control rats, b: Tissue from rats treated with ethylene glycol (EG), ammonium chloride (AC) and 100% lemon juice, c: Tissue from rats treated with EG, AC and 75% lemon juice, d: Tissue from rats treated with EG, AC and 50% lemon juice, e: Tissue from rats treated with EG and AC only (positive control). Crystalline formations in the renal papilla are indicated by arrows. Magnification ×100.
<b>Copyright information:</b>Taken from "Lemon juice has protective activity in ... more <b>Copyright information:</b>Taken from "Lemon juice has protective activity in a rat urolithiasis model"http://www.biomedcentral.com/1471-2490/7/18BMC Urology 2007;7():18-18.Published online 5 Oct 2007PMCID:PMC2194764. Sections were viewed using a BX41 optical microscope and polarized light. a: Tissue from negative control rats, b: Tissue from rats treated with ethylene glycol (EG), ammonium chloride (AC) and 100% lemon juice, c: Tissue from rats treated with EG, AC and 75% lemon juice, d: Tissue from rats treated with EG, AC and 50% lemon juice, e: Tissue from rats treated with EG and AC only (positive control). Crystalline formations in the renal parenchyma are indicated by arrows. Magnification ×100.
<b>Copyright information:</b>Taken from "Lemon juice has protective activity in ... more <b>Copyright information:</b>Taken from "Lemon juice has protective activity in a rat urolithiasis model"http://www.biomedcentral.com/1471-2490/7/18BMC Urology 2007;7():18-18.Published online 5 Oct 2007PMCID:PMC2194764. Values represent mean ± SD (μg/g) for six animals in each group. Values are significantly different from the negative control group: *p < 0.05, **p < 0.01, ***p < 0.001. Values are significantly different from the positive control group: * p < 0.05, **p < 0.01, ***p < 0.001.
The Egyptian Journal of Chemistry, 2021
This study aims to create a model of oleaster oil simply and reliably to detect adulteration, whi... more This study aims to create a model of oleaster oil simply and reliably to detect adulteration, which presents a large danger that attacks the food sector and human health. For this reason, a study to detect the falsification of oleaster oil was carried out by Fourier-Transform Infrared Spectroscopy FTIR and chemometric method. The experimental samples are shared into two sets, 32 Training set, 8 Test set (4 calibration samples opposite one for validation), and a falsification interval of 1.5-40%. The treatment of infrared spectral results has been done by chemometrics techniques utilizing Partial Least Squares regression or Projection to Latent Structures (PLSR) and Principal Component Regression (PCR). The results show that the perfect falsification model of oleaster oil by olive-oil and soybean oil is illustrated in the spectral region 3050-2700 cm, with R of 0.999 from PLSR and PCR to soybean-oil, concerning olive-oil shows also the better results for the PLSR technical with R of ...
Hirri A., De Luca M., Ioele G., Balouki A., Bassbasi M., Kzaiber F., Oussama A., Ragno G. (2015):... more Hirri A., De Luca M., Ioele G., Balouki A., Bassbasi M., Kzaiber F., Oussama A., Ragno G. (2015): Chemometric classification of citrus juices of Moroccan cultivars by infrared spectroscopy. Czech J. Food Sci., 33: 137–142. Fourier transform – infrared (FTIR) spectroscopy in connection with chemometric methodologies was used as a fast and direct analytical approach to classify citrus cultivars by the measurements on their juice. Modern multivariate analysis responds to the current needs for economic, simple, and fast methods able to classify new unknown samples with great accuracy. A set of 135 samples of citrus juice, representative of three cultivars (Hamlin, Muska, and Valencia), all picked in the same geographical area of Morocco, were analysed. Chemometric discrimination of the juice samples was achieved by principal component analysis (PCA) performed on the FTIR spectral data from the juice samples, showing an explained variance of 97.84% by considering only 2 PCs. A fully corr...
Journal of Sensory Studies, 2021
International Journal of Innovation and Applied Studies, 2013
The present work concerns the study of the nutritional quality of the raw milk, on samples result... more The present work concerns the study of the nutritional quality of the raw milk, on samples resulting from centers of collection of the milk (cooperatives) and from farms, from the region of Tadla (Kssiba and Fkih ben Saleh) and the region of Kelaa. The investigations concerned two periods of year 2011 (Period I: February-March-April and Period II: September-October-November). The analysis results show that for the three nutritional parameters, protein content (TP), fat (MG) and defatted dry matter (ESD), there are variations between samples. Assessing the nutritional quality of milk is required to highlight the variability in the nutritional quality of milk production, evaluating the quality of milk produced in those regions and determine the factors influential to improve production and profitability. This quality is demonstrated through the determination of three main parameters of milk constituents, namely fat (MG) Protein content (TP) and nonfat dry (ESD). Milk quality plays an ...
Ensure the quality and authenticity of the products has been a concern of several country. The go... more Ensure the quality and authenticity of the products has been a concern of several country. The goal of this study was to attempt classification of moroccan olives according to their botanical and geographical origin based on the endocarp analysis by Attenuated Total Reflectance-Fourier Transform InfraRed (ATR-FTIR) spectroscopy and find the markers of their authenticity. This study was focused on the olive samples of two varietie (Picholine Marocaine and Arbequina), picked from three farms belong to the three zones: named OuledMbarek, ouledyussuf and Sidijaber, in the Moroccan region of Beni Mellal. The classification models were developed by principal component analysis (PCA) and support vector machines (SVM). On the basis of a PCA, six distinct clusters were recognized. The SVM procedure was then elaborated. The model resulted able to separate the six classes and classify new samples into the appropriate defined classes with a percentage prediction of 95%. This Result show the cap...
Fourier transform–infrared (FTIR) spectroscopy in connection with chemometric methodologies was u... more Fourier transform–infrared (FTIR) spectroscopy in connection with chemometric methodologies was used to develop a fast and direct analytical approach classification model for determining the geographical origins of Navel citrus by the measurements on their juice. Modern multivariate analysis responds to the current needs for economic, simple, and fast methods able to classify new unknown samples with great accuracy. A set of 46 samples of citrus juice, representative of three geographical origins of Morrocco (Beni Mellal, Agadir, and Berkan) were analysed. Chemometric discrimination of the juice samples was achieved by principal component analysis (PCA) performed on the FTIR spectral data from the juice samples, showing an explained variance of 99% by considering only 2 PCs. A fully correct classification of the three geographical origins was then obtained by using Partial Least Square-Discriminant Analysis (PLS-DA) modeling procedure.
Olive oil is a natural product that is used in various fields, such as medicine, cosmetics and nu... more Olive oil is a natural product that is used in various fields, such as medicine, cosmetics and nutrition. It is a food product that is characterized by the multitude of its properties and its economic value. For this reason, it has been adulterated by other vegetable oils which are cheaper and whose properties are similar to those of olive oil and for more the profit. In literature study, there are a lot of works about this oil for determinate and evaluate this authenticity by different ways and techniques. FRIR coupled with Chemometrics has many special advantages because it is having an important characteristic about your manipulation and results. The aim for this review is to evaluate the capacity and the reliability for each method used in the examiner quality of this oil, and to determinate its advantages and disadvantages.
Korean Journal for Food Science of Animal Resources, 2011
Annales d'urologie, 2000
Calculi from 45 Moroccan children aged between 2 and 15 years underwent morphological and infrare... more Calculi from 45 Moroccan children aged between 2 and 15 years underwent morphological and infrared spectrometric analysis. The stones were three times more frequent in males than females (M/F = 3.09). Whewellite was the main component in 51.1% of cases and in 44.4% of stone nuclei, wheddellite in 8.9% of stones and nuclei, carbapatite in 6.7% of stones and 8.9% of nuclei, struvite in 15.6% of stones and 11.1% of nuclei. Ammonium hydrogen urate and uric acid were predominant respectively in 8.9% and 6.7% of stones and in 15.6% and 11.1% of nuclei. In addition to whewellite, struvite and ammonium hydrogen urate were the main components of bladder stones from both sexes. With respect to their calculi, whewellite was present in 84.4% of cases and wheddellite in 26.7%. Purines were present in 46% of calculi, especially as ammonium urate (28.9%) and uric acid (15.6%). Calcium phosphates as the main components were infrequent. In contrast, they were frequently identified in urinary calculi...
Annales d'urologie, 2000
Urolithiasis is a frequent disorder that is characterized by its recurrence following treatment a... more Urolithiasis is a frequent disorder that is characterized by its recurrence following treatment and which can affect between 3-20% of the population, with an incidence that differs from country to country. The aim of the present study was to determine the composition of the calculi, and the remain characteristics of this pathology in Morocco. A series of 80 calculi was therefore analyzed by Fourier transform infrared spectroscopy. The findings showed that calcium monohydrate oxalate was the main lithiasic component, indicating that hyperoxaluria plays a major role in the formation of the calculi. The component identified were as follows: calcium oxalate (58.75%), calcium phosphate and magnesium phosphate (17.5%), uric acid (15%), and urate (8.75%). In 91.25% of cases, the calculi were of mixed composition. Regular patient follow-up is advocated and subjects should be informed of the risk factor involved, as urolithiasic recurrence was observed in 10% of the cases in this series.
Food Chemistry, 2011
Fourier transform infrared (FTIR) spectra were employed for differentiation and classification of... more Fourier transform infrared (FTIR) spectra were employed for differentiation and classification of olive oils from several producing regions of Morocco. A preliminary treatment of the FTIR data was done by a derivative elaboration based on the Savitzky–Golay algorithm to reduce the noise and extract a largest number of analytical information from the spectra. A multivariate statistical procedure based on cluster
Biopolymers and Cell, 1998
Институт молекулярной биологии и генетики НАН Украины 252143, Киев, ул. Академика Заболотного, 15... more Институт молекулярной биологии и генетики НАН Украины 252143, Киев, ул. Академика Заболотного, 150 Проведено сравнение физико-химических свойств тирозил-тРНК синтетаз (КФ 6.1.1.1) из Т. thermophilus и Е. coli Определены величины констант Михаэлиса для тРНК Туг из мезофила и термофила, L-тирозина и АТР в реащии аминоацилирования. тирозил-тРНК синтетазой из Т. thermophilus при 37, 65 и 75 °С, оптимальные концентрации КС1 в реакциях перекрестного аминоацилирования. Осуществлен сравнительньиї анализ аминокислотного состава термофильной и мезофильной тирозил-тРНК синтетаз. Получены температурные зависимости скоростей реакций пирофосфатного обмена и аминоацилирования тРНК, температурные зависимости термостабильности обоих ферментов.
IOSR Journal of Environmental Science, Toxicology and Food Technology, 2016
Recently, sesame oil is highly appreciated by consumers due to its sensory qualities, antioxidant... more Recently, sesame oil is highly appreciated by consumers due to its sensory qualities, antioxidants activity and nutritional benefits, despite its high price. Also, the detection and quantification of adulteration both are problem with increasing importance in the consumer's health and food industry. The goal of this study was to use Fourier transform mid-infrared (FT-MIR) spectroscopy coupled with chemometrics for the quantification of the adulteration of sesame oil. Adulteration with sunflower oil, soybean oil or colza oil is one of the most difficult to detect due to the similar composition of them and sesame oil. Adulterations of sesame oil with different percentages of sunflower oil, soybean oil and colza oil were measured using ATR-FTIR spectroscopy. Spectral and reference data were firstly analyzed by principal component analysis. Partial least square regression (PLSR) was used to establish calibration models. Excellent correlations between ATR-FTIR analysis and studied edible oils concentration was obtained for sunflower oil (R 2 v = 0.997), soybean oil (R 2 v = 0.993) and colza oil (R 2 v = 0.994). PLS models demonstrated the feasibility of quantifiing successfully adulterations of sesame oil in the 0-35 % weight ratio range of edible oils with a rapid and inexpensive tool without the need for sample preparation.
The Egyptian Journal of Chemistry, 2021
The main objective of this study is the use of Fourier transform mid-infrared (FTIR) spectroscopy... more The main objective of this study is the use of Fourier transform mid-infrared (FTIR) spectroscopy for discrimination of samples of Argan kernels from three Moroccan regions. The content of Argan oil extracted from the kernel depends on the plant genotype and on the environmental conditions. The origin of argan kernel samples could be distinguished combining the IR spectra measurement and chemometric tools for discriminant analysis. A multivariate analysis procedure based on the combined use of Hierarchical Cluster Analysis (HCA) and Partial Least Squares-Discriminant Analysis (PLS-DA) was tested for this purpose. HCA showed three distinct clusters related to the Moroccan regions and suggested the usefulness of IR spectroscopy to distinguish among kernel origins. Afterwards, PLS-DA was used for the discrimination and classification of the origin of the various argan kernels and good classification models and validation results were obtained. For 9 new unknown samples we attribuate 10...
FT-IR coupled to chemometrics techniques was used to investigate the quality control of engine lu... more FT-IR coupled to chemometrics techniques was used to investigate the quality control of engine lubricating oils SAE 30 of gear and machines in industries. The results showed that Partial Least Squares Discriminate Analysis (PLS2-DA) gave good classification results with 100% correct class prediction. The proposed procedure is fast, non-destructive, simple and easy to operate. This developed method is highly recommended for quick monitoring of the oil’s quality, and it is another application of FTIR-chemometrics method for quality control of lubricating oils.