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Papers by Abdelsabour Khaled

In order to better understand the relationship genotype-phenotype, marker-trait associations were... more In order to better understand the relationship genotype-phenotype, marker-trait associations were studied in eight wheat genotypes using a set of 95 DNA-based SRAP molecular markers. SRAP analysis showed that the average of the percentage of polymorphism (P%) was 62.59%, as well as the average of polymorphic information content (PIC) was 0.23. Moreover, the means of marker index (MI) was 1.49. The ME1F-EM5R and ME9F-EM3R primers combination showed higher levels of polymorphism of 88.89% and 90.91%, respectively. Single-marker analysis (SMA) indicated that ME-7F-EM-6R1250bp was probably as a candidate marker which linked to 1000-grain weight. In addition, ME-7F-EM-5R900bp and ME-4F-EM-6R490bp SRAP markers were identified for weight of grains/spike and No. of grains/spike, respectively. The cluster analysis based on SRAP and means of morphological data revealed similarity coefficient values ranged from 57.40% to 80.40% and from 90.61% to 98.81%, respectively. Similarity matrices gener...

Morphological traits and molecular markers analyses are very important tools for estimation of ge... more Morphological traits and molecular markers analyses are very important tools for estimation of genetic variability among genotypes. In this study, we combined the phenotypic characterization with the inter-simple sequence repeats (ISSR) technique for the assessment of genetic diversity among 26 bread wheat genotypes. Out of 87 amplified bands 43 were polymorphic. The %P ranged from 25 to 76.92 with an average of 46.97%. The average number of polymorphic bands was 4.30 per primer. UBC-849 and UBC-833 primers which belonged to (GT) and (GA) repeats primer groups produced 62.50 and 76.92% level of polymorphism, respectively. It is clear that the highest polymorphism level was obtained in the case of di-nucleotide repeat primers. In this work the polymorphism information content (PIC) values varied from 0.04 (UBC-819) to 0.19 (UBC-834) with an average of 0.13. The Marker index (MI) values were from 0.12 (UBC-819) to 1.50 (UBC-833) with an average of 0.62. Two markers (UBC-811930bp and U...

... With the advent of electron microscopy in particular the early stages of maize embryogenesis ... more ... With the advent of electron microscopy in particular the early stages of maize embryogenesis were revisited by DIBOLL (1968) and VAN LAMMEREN (1986b), who provided additional insight in the spatial and tem-poral aspects of meristem formation. ...

Assiut Journal of Agricultural Sciences, Dec 1, 2007

The objectives of this work were to analyze the genetic systems controlling variation in some qua... more The objectives of this work were to analyze the genetic systems controlling variation in some quantitative characters (plant height, number of branches/plant, green (immature) pod length, green pod width, number of dry pods/plant, weight of dry seeds/plant and Protein content) in six faba bean lines (Assiut 174, Golden, Assiut 99, Assiut 12 (Romy), Assiut 195 and Assiut 16) and their F 1 's which produced by diallel crossing system. Also, to determine the association between these characters and randomly amplified polymorphic DNA (RAPD) as based similarity estimates. The results indicated that there were significant differences among genotypes in all the studied characters. Heterosis was present for all characters and both additive and nonadditive gene effect were important in the control of these traits. Dominance played the major role in controlling the variation in most the studied traits whereas over-dominance effects were found for number of dry pods/plant, weight of dry seeds/plant and protein content (%). The range of Euclidean distance grouped the six lines into two main clusters, the first cluster contained one line and the other contained the other five lines. Five random primers were used for RAPD experiments that generated 77 fragments with 59 % polymorphism. The range of Nei and li similarity coefficients calculated for the RAPD experiments was 0.55-0.83. The dendrogram based on RAPD marker created two main groups, the first group contained two lines (Assiut 16 and Assiut 195) and the other subdivided into three sub-clusters. The Euclidean distance based on morphological traits was not significantly correlated with the genetic distance based on RAPD markers.

Journal of Environmental Studies

Our study indicates that jojoba is suitable plant for cultivation of the Egyptian marginal soils,... more Our study indicates that jojoba is suitable plant for cultivation of the Egyptian marginal soils, in the desert area, where the seeds were germinated and grown in sandy soil of marginal fertility. To study the effect of NaCl and mannitol on seeds germination, jojoba seeds were placed on cotton layer flooded with solution containing different concentrations of them. Salinity stimulated seed germination, especially, when the seeds were subjected to relatively low concentration of NaCl (0.5-3 gm/l). Increase the concentration of NaCl over 3 gm/l resulted in inhibition of seed germination as well as radicle growth. Application of 2 or 3 gm/l mannitol in germination medium improved the seed germination, vice versa was detected under progressive increase of mannitol in germination medium. Mannitol as same as NaCl delayed seed germination of jojoba plant. On the other hand, jojoba seeds can be germinated in low frequency under high concentration of mannitol, up to 100 gm/l, when seeds were placed on three cotton layers just wetted by distilled water containing mannitol. Temperature may be the most critical factor during jojoba seed germination, therefore summer was the best season for seed germination; also, 30 o C was the best temperature degree for seed germination and emergence of radical in the shortest time.

Journal of Sohag Agriscience (JSAS), 2018

A.G.A. Khaled*, G.A.R. El-Sherbeny, T. M. El-Sheikh and Aml A. Katana Department of Genetics, Fac... more A.G.A. Khaled*, G.A.R. El-Sherbeny, T. M. El-Sheikh and Aml A. Katana Department of Genetics, Faculty of Agriculture, Sohag University, Sohag 82786, Egypt. Department of poultry production, Faculty of Agriculture, Sohag University, Sohag 82786, Egypt. Abstract The study was planned to compare six pure rabbit breeds (Gabali, Chinchilla, V-line – Bouscat, New Zealand White and California) using random amplified polymorphic (RAPD) molecular markers and phenotypic characteristics. A total of 115 amplified bands were scored out of them 98 were polymorphic. The percentage of polymorphism (%P) ranged from 42.86% to 100.00%. The cluster analysis revealed similarity coefficient values ranged from 0.31 (Gabali and California) to 0.66 (Bouscat and New Zealand). A cluster analysis realized using percentage of similarity method for phenotypic data, revealed similarity coefficient values ranged from 77.74 (California and Gabali) to 96.37 (Bouscat and V Line). Results of single marker analysis sho...

Journal of Sohag Agriscience (JSAS), 2017

Genetic parameters, heritabilities and genetic variability were assessed in five tomato genotypes... more Genetic parameters, heritabilities and genetic variability were assessed in five tomato genotypes and their F1 crosses using phenotypic data and RAPD markers under normal and drought stress conditions. The results showed that mean squares of the genotype by environment (GxE) interaction were found to be highly significant for all studied traits, suggesting a differential response of studied genotypes to drought stress. The magnitudes of ơA were higher in magnitude than those of ơ 2 D for most cases indicating that the additive gene action played a major role in the inheritance of these traits. Moreover, the interaction of ơAxE was less than ơDxE for most studied traits, suggesting that the additive gene effect was more stable over the environments than non-additive effect. These results were verified by the estimates of the broadand narrow-sense heritability obtained for different traits. The RAPDs analysis showed that 31 out of 57 bands were polymorphic. The percentage of polymorph...

Journal of Sohag Agriscience (JSAS)

Mean performance and genetic variability were assessed in four soybean genotypes and their F1 cro... more Mean performance and genetic variability were assessed in four soybean genotypes and their F1 crosses using phenotypic data and SRAP markers under drought stress conditions. The results showed that mean squares of G x E interaction were found to be highly significant for most studied traits. Also, the mean performances of four parents and their 6 crosses were variable from normal irrigation to drought stress condition. It could be observed that the parent genotype P2 were relatively stress tolerant parent with the DSI value of 0.5. Using SRAP markers, a total of 49 bands were amplified, of which 24 bands (48.98%) were found polymorphic. Furthermore, the polymorphic band numbers ranged from 2 to 6 bands. The percentage of polymorphism (%P) ranged from 40% (ME2-EM10 primers) to 62% (ME7-EM6 primers) with an average of 48.85%. The SRAP marker ME5-EM1590bp was regarded probably as candidate marker which linked to plant height trait. Interestingly, three different markers (ME1-EM6950bp, EM4-ME61000bp and EM7-ME6970bp) were regarded as candidate markers linked to number of branches per plant. The results showed highly significant and significant regressions (0.3249**, P= 0.013) and (45.04*, P= 0.053) on number of branches per plant and plant height traits, respectively. The UPGMA cluster analysis based on the SRAP markers and the means of morphological traits separated the soybean parental genotypes into two significantly different clusters. Finally, the correlation between the two markers is not significant (r = 0.565, P=0.932).

Journal of Sohag Agriscience (JSAS)

Ten okra genotypes (Abelmoschus esculentus L.) were crossed using half diallel mating design to p... more Ten okra genotypes (Abelmoschus esculentus L.) were crossed using half diallel mating design to produce 45 F1 hybrids. The combining ability and the nature of gene action were determined for economic traits under combined data of tow sowing dates. Genotypic mean squares of were highly significant for all studied traits. Moreover, mean squares due to genotype×environment interaction (G×E) were highly significant for these traits except pod weight, suggesting a differential response of the genotypes from environment to another. The results indicated that the majority of crosses were significantly earlier, taller and higher yielding than their mid parents. Furthermore, there are some crosses showed desirable heterotic values over their better parent for the majority of traits. The results indicated that the magnitudes of the nonadditive genetic variance (σ 2 D) were higher than those of additive ones (σ 2 A) for the majority of studied traits indicating the importance role of non-additive gene action in the inheritance of these traits. However, the magnitudes of σ 2 D×E interaction were more than σ 2 A×E for all studied traits. The largest value of broad sense heritability (98.80%) was recorded for pod weight, while the lowest value (36.22%) was observed for pod length. The estimates of narrow sense heritability ranged from 18.21% to 45.69% for numbers of days to flowering and plant height, respectively. These findings confirmed the predominance of non-additive genetic variance over additive one in the inheritance of these traits. Therefore, the promising crosses which showed desirable specific combining ability (SCA) effects and gave also high estimates of useful heterosis could be utilized for okra hybrids.

SVU-International Journal of Environmental Researches, 2021

A salinity stress problem is defined as a condition where the salts in solution within the crop r... more A salinity stress problem is defined as a condition where the salts in solution within the crop root zone accumulate in high concentrations, which decrease crop yield. Bread wheat (Triticum aestivum. L) is a moderate to salinity tolerance crop Salinity susceptibility index (SSI) become widely used by researchers to identify sensitively and resistant wheat genotypes and increase wheat yield is to improve the salinity tolerance of genotypes. Heterosis represents the percentage of augmenting or decrease in the mean value of the F1 hybrids over their mid-parents’ value. The heterosis represents the percentage of increase or decrease in the mean value of F1 hybrid over a better parent. Knowledge of broad-sense heritability (h2 b.s), narrow-sense heritability (h2 n.s), general combining ability (GCA), and specific combining ability (SCA) is useful in the choice of parental genotypes. Combining ability studies assist in the identification of parents with greater GCA values and parental com...

Generation mean analysis for effect of salinity soil and water was carried out to study days to 5... more Generation mean analysis for effect of salinity soil and water was carried out to study days to 50% Tipping, spike length and No. of spikelets/spike. The results indicated that the mean squares of all genotypes were highly significant for three studied traits, reflecting a great wide genetic variability among them. The mean sum of squares due to the general combining ability (GCA) and specific combining ability (SCA) were high significant for days to 50% Tipping and No. of spikelets/spike, but specific combining ability (SCA) was significant for spike length under salinity stress. Heterosis of all crosses over mid parents (H M.P %) and better parent (H B.P %) for days to 50% Tipping, spike length and No. of spikelets/spike were estimated on this study. The most types of general combining ability effect (gi) and specific combining ability effects (Sij) are involved in the inheritance for three traits. Also, data showed that magnitudes of the non-additive genetic variance (σ2D), which...

Egyptian Journal of Genetics and Cytology, 2016

Phenotypic traits and molecular markers analyses are very important tools for the estimation of g... more Phenotypic traits and molecular markers analyses are very important tools for the estimation of genetic variability among genotypes. In 36 bread wheat genotypes, genetic variability and marker-trait associations were studied for 8 agro-nomic traits using a set of 101 DNA-based molecular markers (61 ISSR and 40 SRAP polymorphic markers). The results of ISSR and SRAP analyses showed similar range of the percentage of polymorphism (%P) (20 to 100%), as well as the average of polymorphic information content (PIC) which was about 0.15 and 0.16, respec-tively. Results showed that di-nucleotide repeat primers represented the highest levels of polymorphism, i.e. UBC-845 and UBC-834 belonged to (CT) and (AG) re-peats produced %P of 92.31 and 100%, respectively. While, UBC-876 belonged tetra-nucleotide repeat (GATA) produced lower level of polymorphism (20%). Re-sults of marker index (MI) showed that SRAP were more efficient than the ISSRs markers, where the SRAP technique ex-hibited higher a...

SVU-International Journal of Agricultural Sciences, 2021

Sequence Related Amplified Polymorphism (SRAP) molecular technique was used for assessing the gen... more Sequence Related Amplified Polymorphism (SRAP) molecular technique was used for assessing the genetic diversity of eight Egyptian bread wheat (Triticum aestivum L.) genotypes. Five SRAP primers combinations were able to expose the polymorphism between the studied genotypes. The percentage of polymorphism (P %) ranged from 40 to 79.90 %. Furthermore, the Polymorphic Information Content (PIC) values for SRAP primers combination varied from 0.11 to 0.31 with an average of 0.19. The Me5F-Em2R and Me1F-Em2R primer combination cleared higher levels of polymorphism of 66.67 and 76.90%, respectively. The results of Single Marker Analysis (SMA) showed that the SRAP marker Me1F-Em2R 1320bp could be considered probably as candidate marker linked to spike length in the tested genotypes. Moreover, Me1F-Em2R 900bp and Me1F-Em2R 575bp could be considered as markers linked to plant height and tillers number /plant traits, respectively. Results of Single Marker Analysis (SMA) showed significant marker-trait associations for spike length (P= 0.04), plant height (P= 0.02) and number of tillers/plant (P= 0.03). Me1F-Em6R 850bp marker could be also considered as candidate markers probably linked to plant height, with a specific fragment of 850bp for the tolerant genotype (P1). UPGMA cluster analysis based on SRAP markers separated the studied wheat genotypes into three significantly different clusters. The dendrogram based on morphological traits divided the studied wheat genotypes into two different clusters. The Mantel test revealed that there was a positive but non-significant correlation between the genetic similarities based on phenotypic data and SRAP marker (r = 0.36, P> 0.05).

Assiut Journal of Agricultural Sciences, 2014

Genetic diversity is one of the key factors for the improvement of many crop plants including sor... more Genetic diversity is one of the key factors for the improvement of many crop plants including sorghum. Fifteen sorghum genotypes were planted in 2012 and 2013 seasons to compare among them for agronomic traits and molecular level. The combined analysis for agronomic traits and yield components showed significant differences among genotypes for all studied traits. The highest mean values of 1000-kernel weight (36.08g) and number of seeds/panicle traits (1315.9) were obtained from Giza-15 genotype. The lowest mean values of 1000kernel weight (17.07g) and number of seeds/panicle traits (646.9) were obtained from local-162 and El-Kharga genotypes, respectively. Values of correlation analysis indicated that 1000-kernel weight was positive and highly significant correlation with grain weight (0.749). The path analysis showed that 1000-kernel weight had high and positive direct effect on grain weight (0.7102), also number of seeds/panicle had positive direct effect on grain weight (0.1443) but it had negative direct effect through plant height (-0.1876). Genetic diversity of sorghum genotypes detected using of Random Amplified Polymorphism DNA (RAPD) markers. 76 DNA bands were obtained from 12 primers which could detected a percentage of polymorphism ranged from 40 to 100% with an average of 73.36%. The average of polymorphic bands was 4.67 per primer. The dendrogram based on RAPD marker gave three main groups; the first group contains three genotypes, but the second group subdivided into two sub-clusters, which contain three genotypes each. The third group contains six genotypes. The similarity percent based on agronomical traits was not significantly correlated (r = 0.07961) with the genetic distance based on RAPD markers.

Journal of Plant Production, 2015

Genetic stability and diversity are two of the key factors for the improvement of many crop plant... more Genetic stability and diversity are two of the key factors for the improvement of many crop plants. A major challenge for plant breeders is selection of high yielding genotype with wide adaptation. Therefore, thirty six wheat genotypes were evaluated under two locations (Sohag and Aswan, Egypt) on favorable and late sowing date during winter seasons of 2012/2013 and 2013/2014 to estimate its performance and stability parameters. The wide range of weather conditions resulted in a broad variation of mean yields, ranging from 6.59 t/ha in favorable sowing date to 4.99 t/ha in late sowing date as heat stress. The combined analysis of variance showed that the flag leaf area, days to heading, spike length, 1000-kernel weight and grain yield were significantly influenced by years, locations, sowing dates and genotypes. Mean environmental grain yield ranged from 2.70 t/ha to 9.27 t/ha. The results showed that sowing at the favorable date increased all studied traits. The 36 genotypes showed diversity for the slopes of the joint regression. Genotypes No. 5, 6, 14, 19, 20, 22, 24 and 32 exhibited stability for grain yield and useful in the breeding program in developing new wheat genotypes with tolerance to heat stress conditions. Positive correlation was found between bi and x for days to heading, spike length, number of kernels/spike, 1000-kernel weight and grain yield (0.89**, 0.50**, 0.07, 0.13 and 0.51**), respectively. This might be due to adaptation of these genotypes to wide differences in climatic conditions which prevailed at the two studied locations. The best genotypes in terms of both favorable and heat stress indicating that selecting for improved yield potential may increase yield in wide range of environments.

Egyptian Journal of Genetics and Cytology, 2013

The EMBO Journal, 2017

Gynogenesis is an asexual mode of reproduction common to animals and plants, in which stimuli fro... more Gynogenesis is an asexual mode of reproduction common to animals and plants, in which stimuli from the sperm cell trigger the development of the unfertilized egg cell into a haploid embryo. Fine mapping restricted a major maize QTL (quantitative trait locus) responsible for the aptitude of inducer lines to trigger gynogenesis to a zone containing a single gene NOT LIKE DAD (NLD) coding for a patatin-like phospholipase A. In all surveyed inducer lines, NLD carries a 4-bp insertion leading to a predicted truncated protein. This frameshift mutation is responsible for haploid induction because complementation with wild-type NLD abolishes the haploid induction capacity. Activity of the NLD promoter is restricted to mature pollen and pollen tube. The translational NLD::citrine fusion protein likely localizes to the sperm cell plasma membrane. In Arabidopsis roots, the truncated protein is no longer localized to the plasma membrane, contrary to the wild-type NLD protein. In conclusion, an intact pollen-specific phospholipase is required for successful sexual reproduction and its targeted disruption may allow establishing powerful haploid breeding tools in numerous crops.

Http Www Theses Fr, 2005

LYON-ENS Sciences (693872304) / SudocSudocFranceF

In order to better understand the relationship genotype-phenotype, marker-trait associations were... more In order to better understand the relationship genotype-phenotype, marker-trait associations were studied in eight wheat genotypes using a set of 95 DNA-based SRAP molecular markers. SRAP analysis showed that the average of the percentage of polymorphism (P%) was 62.59%, as well as the average of polymorphic information content (PIC) was 0.23. Moreover, the means of marker index (MI) was 1.49. The ME1F-EM5R and ME9F-EM3R primers combination showed higher levels of polymorphism of 88.89% and 90.91%, respectively. Single-marker analysis (SMA) indicated that ME-7F-EM-6R1250bp was probably as a candidate marker which linked to 1000-grain weight. In addition, ME-7F-EM-5R900bp and ME-4F-EM-6R490bp SRAP markers were identified for weight of grains/spike and No. of grains/spike, respectively. The cluster analysis based on SRAP and means of morphological data revealed similarity coefficient values ranged from 57.40% to 80.40% and from 90.61% to 98.81%, respectively. Similarity matrices gener...

Morphological traits and molecular markers analyses are very important tools for estimation of ge... more Morphological traits and molecular markers analyses are very important tools for estimation of genetic variability among genotypes. In this study, we combined the phenotypic characterization with the inter-simple sequence repeats (ISSR) technique for the assessment of genetic diversity among 26 bread wheat genotypes. Out of 87 amplified bands 43 were polymorphic. The %P ranged from 25 to 76.92 with an average of 46.97%. The average number of polymorphic bands was 4.30 per primer. UBC-849 and UBC-833 primers which belonged to (GT) and (GA) repeats primer groups produced 62.50 and 76.92% level of polymorphism, respectively. It is clear that the highest polymorphism level was obtained in the case of di-nucleotide repeat primers. In this work the polymorphism information content (PIC) values varied from 0.04 (UBC-819) to 0.19 (UBC-834) with an average of 0.13. The Marker index (MI) values were from 0.12 (UBC-819) to 1.50 (UBC-833) with an average of 0.62. Two markers (UBC-811930bp and U...

... With the advent of electron microscopy in particular the early stages of maize embryogenesis ... more ... With the advent of electron microscopy in particular the early stages of maize embryogenesis were revisited by DIBOLL (1968) and VAN LAMMEREN (1986b), who provided additional insight in the spatial and tem-poral aspects of meristem formation. ...

Assiut Journal of Agricultural Sciences, Dec 1, 2007

The objectives of this work were to analyze the genetic systems controlling variation in some qua... more The objectives of this work were to analyze the genetic systems controlling variation in some quantitative characters (plant height, number of branches/plant, green (immature) pod length, green pod width, number of dry pods/plant, weight of dry seeds/plant and Protein content) in six faba bean lines (Assiut 174, Golden, Assiut 99, Assiut 12 (Romy), Assiut 195 and Assiut 16) and their F 1 's which produced by diallel crossing system. Also, to determine the association between these characters and randomly amplified polymorphic DNA (RAPD) as based similarity estimates. The results indicated that there were significant differences among genotypes in all the studied characters. Heterosis was present for all characters and both additive and nonadditive gene effect were important in the control of these traits. Dominance played the major role in controlling the variation in most the studied traits whereas over-dominance effects were found for number of dry pods/plant, weight of dry seeds/plant and protein content (%). The range of Euclidean distance grouped the six lines into two main clusters, the first cluster contained one line and the other contained the other five lines. Five random primers were used for RAPD experiments that generated 77 fragments with 59 % polymorphism. The range of Nei and li similarity coefficients calculated for the RAPD experiments was 0.55-0.83. The dendrogram based on RAPD marker created two main groups, the first group contained two lines (Assiut 16 and Assiut 195) and the other subdivided into three sub-clusters. The Euclidean distance based on morphological traits was not significantly correlated with the genetic distance based on RAPD markers.

Journal of Environmental Studies

Our study indicates that jojoba is suitable plant for cultivation of the Egyptian marginal soils,... more Our study indicates that jojoba is suitable plant for cultivation of the Egyptian marginal soils, in the desert area, where the seeds were germinated and grown in sandy soil of marginal fertility. To study the effect of NaCl and mannitol on seeds germination, jojoba seeds were placed on cotton layer flooded with solution containing different concentrations of them. Salinity stimulated seed germination, especially, when the seeds were subjected to relatively low concentration of NaCl (0.5-3 gm/l). Increase the concentration of NaCl over 3 gm/l resulted in inhibition of seed germination as well as radicle growth. Application of 2 or 3 gm/l mannitol in germination medium improved the seed germination, vice versa was detected under progressive increase of mannitol in germination medium. Mannitol as same as NaCl delayed seed germination of jojoba plant. On the other hand, jojoba seeds can be germinated in low frequency under high concentration of mannitol, up to 100 gm/l, when seeds were placed on three cotton layers just wetted by distilled water containing mannitol. Temperature may be the most critical factor during jojoba seed germination, therefore summer was the best season for seed germination; also, 30 o C was the best temperature degree for seed germination and emergence of radical in the shortest time.

Journal of Sohag Agriscience (JSAS), 2018

A.G.A. Khaled*, G.A.R. El-Sherbeny, T. M. El-Sheikh and Aml A. Katana Department of Genetics, Fac... more A.G.A. Khaled*, G.A.R. El-Sherbeny, T. M. El-Sheikh and Aml A. Katana Department of Genetics, Faculty of Agriculture, Sohag University, Sohag 82786, Egypt. Department of poultry production, Faculty of Agriculture, Sohag University, Sohag 82786, Egypt. Abstract The study was planned to compare six pure rabbit breeds (Gabali, Chinchilla, V-line – Bouscat, New Zealand White and California) using random amplified polymorphic (RAPD) molecular markers and phenotypic characteristics. A total of 115 amplified bands were scored out of them 98 were polymorphic. The percentage of polymorphism (%P) ranged from 42.86% to 100.00%. The cluster analysis revealed similarity coefficient values ranged from 0.31 (Gabali and California) to 0.66 (Bouscat and New Zealand). A cluster analysis realized using percentage of similarity method for phenotypic data, revealed similarity coefficient values ranged from 77.74 (California and Gabali) to 96.37 (Bouscat and V Line). Results of single marker analysis sho...

Journal of Sohag Agriscience (JSAS), 2017

Genetic parameters, heritabilities and genetic variability were assessed in five tomato genotypes... more Genetic parameters, heritabilities and genetic variability were assessed in five tomato genotypes and their F1 crosses using phenotypic data and RAPD markers under normal and drought stress conditions. The results showed that mean squares of the genotype by environment (GxE) interaction were found to be highly significant for all studied traits, suggesting a differential response of studied genotypes to drought stress. The magnitudes of ơA were higher in magnitude than those of ơ 2 D for most cases indicating that the additive gene action played a major role in the inheritance of these traits. Moreover, the interaction of ơAxE was less than ơDxE for most studied traits, suggesting that the additive gene effect was more stable over the environments than non-additive effect. These results were verified by the estimates of the broadand narrow-sense heritability obtained for different traits. The RAPDs analysis showed that 31 out of 57 bands were polymorphic. The percentage of polymorph...

Journal of Sohag Agriscience (JSAS)

Mean performance and genetic variability were assessed in four soybean genotypes and their F1 cro... more Mean performance and genetic variability were assessed in four soybean genotypes and their F1 crosses using phenotypic data and SRAP markers under drought stress conditions. The results showed that mean squares of G x E interaction were found to be highly significant for most studied traits. Also, the mean performances of four parents and their 6 crosses were variable from normal irrigation to drought stress condition. It could be observed that the parent genotype P2 were relatively stress tolerant parent with the DSI value of 0.5. Using SRAP markers, a total of 49 bands were amplified, of which 24 bands (48.98%) were found polymorphic. Furthermore, the polymorphic band numbers ranged from 2 to 6 bands. The percentage of polymorphism (%P) ranged from 40% (ME2-EM10 primers) to 62% (ME7-EM6 primers) with an average of 48.85%. The SRAP marker ME5-EM1590bp was regarded probably as candidate marker which linked to plant height trait. Interestingly, three different markers (ME1-EM6950bp, EM4-ME61000bp and EM7-ME6970bp) were regarded as candidate markers linked to number of branches per plant. The results showed highly significant and significant regressions (0.3249**, P= 0.013) and (45.04*, P= 0.053) on number of branches per plant and plant height traits, respectively. The UPGMA cluster analysis based on the SRAP markers and the means of morphological traits separated the soybean parental genotypes into two significantly different clusters. Finally, the correlation between the two markers is not significant (r = 0.565, P=0.932).

Journal of Sohag Agriscience (JSAS)

Ten okra genotypes (Abelmoschus esculentus L.) were crossed using half diallel mating design to p... more Ten okra genotypes (Abelmoschus esculentus L.) were crossed using half diallel mating design to produce 45 F1 hybrids. The combining ability and the nature of gene action were determined for economic traits under combined data of tow sowing dates. Genotypic mean squares of were highly significant for all studied traits. Moreover, mean squares due to genotype×environment interaction (G×E) were highly significant for these traits except pod weight, suggesting a differential response of the genotypes from environment to another. The results indicated that the majority of crosses were significantly earlier, taller and higher yielding than their mid parents. Furthermore, there are some crosses showed desirable heterotic values over their better parent for the majority of traits. The results indicated that the magnitudes of the nonadditive genetic variance (σ 2 D) were higher than those of additive ones (σ 2 A) for the majority of studied traits indicating the importance role of non-additive gene action in the inheritance of these traits. However, the magnitudes of σ 2 D×E interaction were more than σ 2 A×E for all studied traits. The largest value of broad sense heritability (98.80%) was recorded for pod weight, while the lowest value (36.22%) was observed for pod length. The estimates of narrow sense heritability ranged from 18.21% to 45.69% for numbers of days to flowering and plant height, respectively. These findings confirmed the predominance of non-additive genetic variance over additive one in the inheritance of these traits. Therefore, the promising crosses which showed desirable specific combining ability (SCA) effects and gave also high estimates of useful heterosis could be utilized for okra hybrids.

SVU-International Journal of Environmental Researches, 2021

A salinity stress problem is defined as a condition where the salts in solution within the crop r... more A salinity stress problem is defined as a condition where the salts in solution within the crop root zone accumulate in high concentrations, which decrease crop yield. Bread wheat (Triticum aestivum. L) is a moderate to salinity tolerance crop Salinity susceptibility index (SSI) become widely used by researchers to identify sensitively and resistant wheat genotypes and increase wheat yield is to improve the salinity tolerance of genotypes. Heterosis represents the percentage of augmenting or decrease in the mean value of the F1 hybrids over their mid-parents’ value. The heterosis represents the percentage of increase or decrease in the mean value of F1 hybrid over a better parent. Knowledge of broad-sense heritability (h2 b.s), narrow-sense heritability (h2 n.s), general combining ability (GCA), and specific combining ability (SCA) is useful in the choice of parental genotypes. Combining ability studies assist in the identification of parents with greater GCA values and parental com...

Generation mean analysis for effect of salinity soil and water was carried out to study days to 5... more Generation mean analysis for effect of salinity soil and water was carried out to study days to 50% Tipping, spike length and No. of spikelets/spike. The results indicated that the mean squares of all genotypes were highly significant for three studied traits, reflecting a great wide genetic variability among them. The mean sum of squares due to the general combining ability (GCA) and specific combining ability (SCA) were high significant for days to 50% Tipping and No. of spikelets/spike, but specific combining ability (SCA) was significant for spike length under salinity stress. Heterosis of all crosses over mid parents (H M.P %) and better parent (H B.P %) for days to 50% Tipping, spike length and No. of spikelets/spike were estimated on this study. The most types of general combining ability effect (gi) and specific combining ability effects (Sij) are involved in the inheritance for three traits. Also, data showed that magnitudes of the non-additive genetic variance (σ2D), which...

Egyptian Journal of Genetics and Cytology, 2016

Phenotypic traits and molecular markers analyses are very important tools for the estimation of g... more Phenotypic traits and molecular markers analyses are very important tools for the estimation of genetic variability among genotypes. In 36 bread wheat genotypes, genetic variability and marker-trait associations were studied for 8 agro-nomic traits using a set of 101 DNA-based molecular markers (61 ISSR and 40 SRAP polymorphic markers). The results of ISSR and SRAP analyses showed similar range of the percentage of polymorphism (%P) (20 to 100%), as well as the average of polymorphic information content (PIC) which was about 0.15 and 0.16, respec-tively. Results showed that di-nucleotide repeat primers represented the highest levels of polymorphism, i.e. UBC-845 and UBC-834 belonged to (CT) and (AG) re-peats produced %P of 92.31 and 100%, respectively. While, UBC-876 belonged tetra-nucleotide repeat (GATA) produced lower level of polymorphism (20%). Re-sults of marker index (MI) showed that SRAP were more efficient than the ISSRs markers, where the SRAP technique ex-hibited higher a...

SVU-International Journal of Agricultural Sciences, 2021

Sequence Related Amplified Polymorphism (SRAP) molecular technique was used for assessing the gen... more Sequence Related Amplified Polymorphism (SRAP) molecular technique was used for assessing the genetic diversity of eight Egyptian bread wheat (Triticum aestivum L.) genotypes. Five SRAP primers combinations were able to expose the polymorphism between the studied genotypes. The percentage of polymorphism (P %) ranged from 40 to 79.90 %. Furthermore, the Polymorphic Information Content (PIC) values for SRAP primers combination varied from 0.11 to 0.31 with an average of 0.19. The Me5F-Em2R and Me1F-Em2R primer combination cleared higher levels of polymorphism of 66.67 and 76.90%, respectively. The results of Single Marker Analysis (SMA) showed that the SRAP marker Me1F-Em2R 1320bp could be considered probably as candidate marker linked to spike length in the tested genotypes. Moreover, Me1F-Em2R 900bp and Me1F-Em2R 575bp could be considered as markers linked to plant height and tillers number /plant traits, respectively. Results of Single Marker Analysis (SMA) showed significant marker-trait associations for spike length (P= 0.04), plant height (P= 0.02) and number of tillers/plant (P= 0.03). Me1F-Em6R 850bp marker could be also considered as candidate markers probably linked to plant height, with a specific fragment of 850bp for the tolerant genotype (P1). UPGMA cluster analysis based on SRAP markers separated the studied wheat genotypes into three significantly different clusters. The dendrogram based on morphological traits divided the studied wheat genotypes into two different clusters. The Mantel test revealed that there was a positive but non-significant correlation between the genetic similarities based on phenotypic data and SRAP marker (r = 0.36, P> 0.05).

Assiut Journal of Agricultural Sciences, 2014

Genetic diversity is one of the key factors for the improvement of many crop plants including sor... more Genetic diversity is one of the key factors for the improvement of many crop plants including sorghum. Fifteen sorghum genotypes were planted in 2012 and 2013 seasons to compare among them for agronomic traits and molecular level. The combined analysis for agronomic traits and yield components showed significant differences among genotypes for all studied traits. The highest mean values of 1000-kernel weight (36.08g) and number of seeds/panicle traits (1315.9) were obtained from Giza-15 genotype. The lowest mean values of 1000kernel weight (17.07g) and number of seeds/panicle traits (646.9) were obtained from local-162 and El-Kharga genotypes, respectively. Values of correlation analysis indicated that 1000-kernel weight was positive and highly significant correlation with grain weight (0.749). The path analysis showed that 1000-kernel weight had high and positive direct effect on grain weight (0.7102), also number of seeds/panicle had positive direct effect on grain weight (0.1443) but it had negative direct effect through plant height (-0.1876). Genetic diversity of sorghum genotypes detected using of Random Amplified Polymorphism DNA (RAPD) markers. 76 DNA bands were obtained from 12 primers which could detected a percentage of polymorphism ranged from 40 to 100% with an average of 73.36%. The average of polymorphic bands was 4.67 per primer. The dendrogram based on RAPD marker gave three main groups; the first group contains three genotypes, but the second group subdivided into two sub-clusters, which contain three genotypes each. The third group contains six genotypes. The similarity percent based on agronomical traits was not significantly correlated (r = 0.07961) with the genetic distance based on RAPD markers.

Journal of Plant Production, 2015

Genetic stability and diversity are two of the key factors for the improvement of many crop plant... more Genetic stability and diversity are two of the key factors for the improvement of many crop plants. A major challenge for plant breeders is selection of high yielding genotype with wide adaptation. Therefore, thirty six wheat genotypes were evaluated under two locations (Sohag and Aswan, Egypt) on favorable and late sowing date during winter seasons of 2012/2013 and 2013/2014 to estimate its performance and stability parameters. The wide range of weather conditions resulted in a broad variation of mean yields, ranging from 6.59 t/ha in favorable sowing date to 4.99 t/ha in late sowing date as heat stress. The combined analysis of variance showed that the flag leaf area, days to heading, spike length, 1000-kernel weight and grain yield were significantly influenced by years, locations, sowing dates and genotypes. Mean environmental grain yield ranged from 2.70 t/ha to 9.27 t/ha. The results showed that sowing at the favorable date increased all studied traits. The 36 genotypes showed diversity for the slopes of the joint regression. Genotypes No. 5, 6, 14, 19, 20, 22, 24 and 32 exhibited stability for grain yield and useful in the breeding program in developing new wheat genotypes with tolerance to heat stress conditions. Positive correlation was found between bi and x for days to heading, spike length, number of kernels/spike, 1000-kernel weight and grain yield (0.89**, 0.50**, 0.07, 0.13 and 0.51**), respectively. This might be due to adaptation of these genotypes to wide differences in climatic conditions which prevailed at the two studied locations. The best genotypes in terms of both favorable and heat stress indicating that selecting for improved yield potential may increase yield in wide range of environments.

Egyptian Journal of Genetics and Cytology, 2013

The EMBO Journal, 2017

Gynogenesis is an asexual mode of reproduction common to animals and plants, in which stimuli fro... more Gynogenesis is an asexual mode of reproduction common to animals and plants, in which stimuli from the sperm cell trigger the development of the unfertilized egg cell into a haploid embryo. Fine mapping restricted a major maize QTL (quantitative trait locus) responsible for the aptitude of inducer lines to trigger gynogenesis to a zone containing a single gene NOT LIKE DAD (NLD) coding for a patatin-like phospholipase A. In all surveyed inducer lines, NLD carries a 4-bp insertion leading to a predicted truncated protein. This frameshift mutation is responsible for haploid induction because complementation with wild-type NLD abolishes the haploid induction capacity. Activity of the NLD promoter is restricted to mature pollen and pollen tube. The translational NLD::citrine fusion protein likely localizes to the sperm cell plasma membrane. In Arabidopsis roots, the truncated protein is no longer localized to the plasma membrane, contrary to the wild-type NLD protein. In conclusion, an intact pollen-specific phospholipase is required for successful sexual reproduction and its targeted disruption may allow establishing powerful haploid breeding tools in numerous crops.

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