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Papers by Abdul Onoruoiza Momoh

Research paper thumbnail of Molecular analysis of the resistant factor of virulent uropathogenic Escherichia coli in volunteered females of Elizade University

Microbes and Infectious Diseases (Print), Mar 11, 2022

Background: The antibiotic resistance, plasmid profile and virulence of Escherichia coli isolated... more Background: The antibiotic resistance, plasmid profile and virulence of Escherichia coli isolated from volunteered females of Elizade University, Ilara-Mokin, Ondo State was studied within a period of twenty months along with a questioner. Methods: A total of 300 samples of urine were collected and processed using standard microbiological culturing techniques. Identification, antibiotic susceptibility (disc diffusion), plasmid analysis, detection of virulence gene and curing of virulence were carried out. Results: The result showed a prevalence rate of 10.5% significant bacteriuria in sexually transmitted disease case. Majority (72.4%) were between 16-22 years (30.25±4.58) and 81% of the students had well educated parents. On a general note, Eschericia coli accounted for 42.5% of the bacteria isolated followed by Staphylococcus aureus (32.5%). All the uropathogens isolated were not susceptible to the commonly prescribed antibiotics; cefuroxime (100%) but mostly susceptible to quinolone (84%) and cephalosporin (81%). Of the twenty-five (25) E. coli isolated from these urine samples, fifteen (15) possess the ability to produce beta-lactamase enzyme. Eleven of these fifteen E. coli isolates (11/15%) were positive for type 1 pili fimbriae adhesin virulence factor and one showed the presence of shiga toxin 1. Ten of these E. coli had plasmid resistance gene. The curing of these resistant plasmid using high temperature incubation (at 42 0 C) showed that six were cured of their resistance. Conclusion: These results showed that there has been no significant change in the prevalence of bacteriuria in the recent years. Guideline to prevent indiscriminate use of antibiotics is needed to prevent the resistance observed in this study.

Research paper thumbnail of The comparative antimicrobial effects of castor, garlic, beniseed and bitter cola extracts on microorganisms isolated from hospitals’ wards

Microbes and Infectious Diseases (Print), May 22, 2023

Background Medically, hospital acquired infection or nosocomial, have been defined by various aut... more Background Medically, hospital acquired infection or nosocomial, have been defined by various authors in different ways as dim fit probably based on their studies. However, in broad terms, it is an infection whose development is aided or favored by the hospital environment. This may be due to the fact The comparative antimicrobial effects of castor, garlic, beniseed and bitter cola extracts on microorganisms isolated from hospitals' wards

Research paper thumbnail of In vivo Evaluation of Microorganisms Isolated from Peels of Selected Carbohydrate Rich Tubers

British Microbiology Research Journal, 2016

Aims: To detect the effect of microbes isolated from peels of Carbohydrate rich tubers on Albino ... more Aims: To detect the effect of microbes isolated from peels of Carbohydrate rich tubers on Albino rats. Study Design: The study was a comparative and descriptive study. Cassava and cocoyam peels were bought from Obanla market, Federal University of Technology, Akure. Those samples were put into a sterile polythene bag and transported to microbiology laboratory for microbiological analysis.

Research paper thumbnail of Haematological and Pathological Effects of Bacteria from Vegetable Wastes in Ilara-Mokin, Ondo State

Microbiology Research Journal International, 2018

Aims: Different selling points or markets in Ilara-Mokin town were visited after the market sessi... more Aims: Different selling points or markets in Ilara-Mokin town were visited after the market session and a day after to collect vegetable wastes. This research aimed to isolate bacteria and fungi from selected waste vegetables, to infect laboratory animals with the bacterial isolates and to study the effect of the isolated bacteria on the laboratory animals. Methodology: Three major vegetable wastes were commonly found in the markets and they are Amaranthus cruentus (Arowojeja), Senecio biafrae (Worowo) and Spinacia oleracea (Amunututu). Sufficient quantity of these decaying wastes were collected and sterile crucible was used to grind the waste samples. On each sample, serial dilution was done using 2g. Nutrient Agar and potato dextrose agar were used for isolation of bacteria and fungi respectively. Standard Microbiological methods were used for the identification of the microorganisms.

Research paper thumbnail of The Antibacterial and Phytochemical Evaluation of Extracts of Grape (Vitis Vinifera) Seeds

Agriculture and Natural Resources, 2015

The skins and seeds of grapes are known to be rich sources of phenolic compounds, both flavonoids... more The skins and seeds of grapes are known to be rich sources of phenolic compounds, both flavonoids and non-flavonoids. These compounds, when present in plants have been confirmed to have antibacterial property. The antibacterial and phytochemical evaluation of grape seeds was done in vitro on selected bacteria ( Bacillus cereus, Escherichia coli, Proteus vulgaris, Staphylococcus aureus, Shigella dysenteriae and Salmonella typhi ). The seeds were collected from a local farmer in Ijare, Ondo State, dried and extracted using 98% ethanol and methanol respectively after blending the seeds to powder. The qualitative phytochemical analysis of the seeds was done using standard methods. The results showed that the methanol extract was more effective than the ethanol extract and had the highest diameter of zone of inhibition of 16.90mm on Staphylococcus aureus. The methanol extract had an average minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 25mg/ml on...

Research paper thumbnail of In vivo Evaluation of Microorganisms Isolated from Peels of Selected Carbohydrate Rich Tubers

Aims: To detect the effect of microbes isolated from peels of Carbohydrate rich tubers on Albino ... more Aims: To detect the effect of microbes isolated from peels of Carbohydrate rich tubers on Albino rats. Study Design: The study was a comparative and descriptive study. Cassava and cocoyam peels were bought from Obanla market, Federal University of Technology, Akure. Those samples were put into a sterile polythene bag and transported to microbiology laboratory for microbiological analysis. Methodology: Two gram of the samples was grounded in a mortar with pestle. The serial dilution method was aseptically used to reduce the microbial load present in the collected samples. Nutrient agar and potato dextrose agar were used to isolate the bacteria and fungi respectively from the samples under aseptic condition. Biochemical test and sugar fermentation were carried out on the bacterial isolates after pure culture was obtained. Results: The isolated bacteria were Streptococcus faecalis, Corynebacterium fascians, Micrococcus luteus, Alcaligenes faecalis and Aeromonas hydrophila. Fungal isola...

Research paper thumbnail of Molecular analysis of the resistant factor of virulent uropathogenic Escherichia coli in volunteered females of Elizade University

Microbes and Infectious Diseases (Print), Mar 11, 2022

Background: The antibiotic resistance, plasmid profile and virulence of Escherichia coli isolated... more Background: The antibiotic resistance, plasmid profile and virulence of Escherichia coli isolated from volunteered females of Elizade University, Ilara-Mokin, Ondo State was studied within a period of twenty months along with a questioner. Methods: A total of 300 samples of urine were collected and processed using standard microbiological culturing techniques. Identification, antibiotic susceptibility (disc diffusion), plasmid analysis, detection of virulence gene and curing of virulence were carried out. Results: The result showed a prevalence rate of 10.5% significant bacteriuria in sexually transmitted disease case. Majority (72.4%) were between 16-22 years (30.25±4.58) and 81% of the students had well educated parents. On a general note, Eschericia coli accounted for 42.5% of the bacteria isolated followed by Staphylococcus aureus (32.5%). All the uropathogens isolated were not susceptible to the commonly prescribed antibiotics; cefuroxime (100%) but mostly susceptible to quinolone (84%) and cephalosporin (81%). Of the twenty-five (25) E. coli isolated from these urine samples, fifteen (15) possess the ability to produce beta-lactamase enzyme. Eleven of these fifteen E. coli isolates (11/15%) were positive for type 1 pili fimbriae adhesin virulence factor and one showed the presence of shiga toxin 1. Ten of these E. coli had plasmid resistance gene. The curing of these resistant plasmid using high temperature incubation (at 42 0 C) showed that six were cured of their resistance. Conclusion: These results showed that there has been no significant change in the prevalence of bacteriuria in the recent years. Guideline to prevent indiscriminate use of antibiotics is needed to prevent the resistance observed in this study.

Research paper thumbnail of The comparative antimicrobial effects of castor, garlic, beniseed and bitter cola extracts on microorganisms isolated from hospitals’ wards

Microbes and Infectious Diseases (Print), May 22, 2023

Background Medically, hospital acquired infection or nosocomial, have been defined by various aut... more Background Medically, hospital acquired infection or nosocomial, have been defined by various authors in different ways as dim fit probably based on their studies. However, in broad terms, it is an infection whose development is aided or favored by the hospital environment. This may be due to the fact The comparative antimicrobial effects of castor, garlic, beniseed and bitter cola extracts on microorganisms isolated from hospitals' wards

Research paper thumbnail of In vivo Evaluation of Microorganisms Isolated from Peels of Selected Carbohydrate Rich Tubers

British Microbiology Research Journal, 2016

Aims: To detect the effect of microbes isolated from peels of Carbohydrate rich tubers on Albino ... more Aims: To detect the effect of microbes isolated from peels of Carbohydrate rich tubers on Albino rats. Study Design: The study was a comparative and descriptive study. Cassava and cocoyam peels were bought from Obanla market, Federal University of Technology, Akure. Those samples were put into a sterile polythene bag and transported to microbiology laboratory for microbiological analysis.

Research paper thumbnail of Haematological and Pathological Effects of Bacteria from Vegetable Wastes in Ilara-Mokin, Ondo State

Microbiology Research Journal International, 2018

Aims: Different selling points or markets in Ilara-Mokin town were visited after the market sessi... more Aims: Different selling points or markets in Ilara-Mokin town were visited after the market session and a day after to collect vegetable wastes. This research aimed to isolate bacteria and fungi from selected waste vegetables, to infect laboratory animals with the bacterial isolates and to study the effect of the isolated bacteria on the laboratory animals. Methodology: Three major vegetable wastes were commonly found in the markets and they are Amaranthus cruentus (Arowojeja), Senecio biafrae (Worowo) and Spinacia oleracea (Amunututu). Sufficient quantity of these decaying wastes were collected and sterile crucible was used to grind the waste samples. On each sample, serial dilution was done using 2g. Nutrient Agar and potato dextrose agar were used for isolation of bacteria and fungi respectively. Standard Microbiological methods were used for the identification of the microorganisms.

Research paper thumbnail of The Antibacterial and Phytochemical Evaluation of Extracts of Grape (Vitis Vinifera) Seeds

Agriculture and Natural Resources, 2015

The skins and seeds of grapes are known to be rich sources of phenolic compounds, both flavonoids... more The skins and seeds of grapes are known to be rich sources of phenolic compounds, both flavonoids and non-flavonoids. These compounds, when present in plants have been confirmed to have antibacterial property. The antibacterial and phytochemical evaluation of grape seeds was done in vitro on selected bacteria ( Bacillus cereus, Escherichia coli, Proteus vulgaris, Staphylococcus aureus, Shigella dysenteriae and Salmonella typhi ). The seeds were collected from a local farmer in Ijare, Ondo State, dried and extracted using 98% ethanol and methanol respectively after blending the seeds to powder. The qualitative phytochemical analysis of the seeds was done using standard methods. The results showed that the methanol extract was more effective than the ethanol extract and had the highest diameter of zone of inhibition of 16.90mm on Staphylococcus aureus. The methanol extract had an average minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 25mg/ml on...

Research paper thumbnail of In vivo Evaluation of Microorganisms Isolated from Peels of Selected Carbohydrate Rich Tubers

Aims: To detect the effect of microbes isolated from peels of Carbohydrate rich tubers on Albino ... more Aims: To detect the effect of microbes isolated from peels of Carbohydrate rich tubers on Albino rats. Study Design: The study was a comparative and descriptive study. Cassava and cocoyam peels were bought from Obanla market, Federal University of Technology, Akure. Those samples were put into a sterile polythene bag and transported to microbiology laboratory for microbiological analysis. Methodology: Two gram of the samples was grounded in a mortar with pestle. The serial dilution method was aseptically used to reduce the microbial load present in the collected samples. Nutrient agar and potato dextrose agar were used to isolate the bacteria and fungi respectively from the samples under aseptic condition. Biochemical test and sugar fermentation were carried out on the bacterial isolates after pure culture was obtained. Results: The isolated bacteria were Streptococcus faecalis, Corynebacterium fascians, Micrococcus luteus, Alcaligenes faecalis and Aeromonas hydrophila. Fungal isola...