Abdul Rauf Khaskheli - Academia.edu (original) (raw)
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Papers by Abdul Rauf Khaskheli
Vibrational Spectroscopy, 2011
Poultry feed lipid n − 3 n − 6 fatty acids n − 6:n − 3 ratio FTIR spectroscopy Partial least squa... more Poultry feed lipid n − 3 n − 6 fatty acids n − 6:n − 3 ratio FTIR spectroscopy Partial least squares a b s t r a c t A rapid, simple and reproducible single bounce attenuated total reflectance (SB-ATR) Fourier transform infrared (FTIR) spectroscopic method was developed for determination of the n − 6, n − 3 and ratio of n − 6:n − 3 polyunsaturated fatty acids (PUFAs) in poultry feed lipids using partial least squares (PLS) regression. Data for n − 6, n − 3 and ratio of n − 6:n − 3 was acquired by gas chromatography (GC) and used as a standard values for FTIR calibration. The best regression results were achieved using first derivatives of the 1475-650 cm −1 spectral region for n − 6, n − 3 and ratio of n − 6:n − 3 with high regression coefficients (R 2 ) of 0.999, 0.994 and 0.998, respectively and low RMSEP values of 1, 0.06 and 0.83, respectively. The results of the present study revealed that FTIR could be used for rapid and accurate determination of n − 6, n − 3 and ratio of n − 6:n − 3 PUFAs present in poultry feed lipids.
Spectrochimica Acta Part A-molecular and Biomolecular Spectroscopy, 2007
A very fast, economical and simpler direct spectrophotometric method was investigated for paracet... more A very fast, economical and simpler direct spectrophotometric method was investigated for paracetamol (PC) determination in aqueous medium without using any chemical reagents. The method is based on the photo-absorption of the analyte at 243 nm after dissolution in water. The change in structure of PC after addition of water was studied by comparing the corresponding FTIR spectra. Optimization studies were conducted by using a 5 microg ml(-1) standard solution of the analyte. Various parameters studied include, time for stability and measurement of spectra, effect of HCl, NaOH, CH(3)COOH and NH(3) for change in absorbance and shift in spectra, interference by some analgesic drugs and some polar solvents and temperature effect. After optimization, Beer's law was obeyed in the range of 0.3-20 microg ml(-1) PC solution with a correlation coefficient of 0.9999 and detection limit of 0.1 microg ml(-1). The newly developed method was successfully applied for PC determination in some locally available tablets and urine samples. The proposed method is very useful for quick analysis of various types of solid and liquid samples containing PC.
Vibrational Spectroscopy, 2011
Poultry feed lipid n − 3 n − 6 fatty acids n − 6:n − 3 ratio FTIR spectroscopy Partial least squa... more Poultry feed lipid n − 3 n − 6 fatty acids n − 6:n − 3 ratio FTIR spectroscopy Partial least squares a b s t r a c t A rapid, simple and reproducible single bounce attenuated total reflectance (SB-ATR) Fourier transform infrared (FTIR) spectroscopic method was developed for determination of the n − 6, n − 3 and ratio of n − 6:n − 3 polyunsaturated fatty acids (PUFAs) in poultry feed lipids using partial least squares (PLS) regression. Data for n − 6, n − 3 and ratio of n − 6:n − 3 was acquired by gas chromatography (GC) and used as a standard values for FTIR calibration. The best regression results were achieved using first derivatives of the 1475-650 cm −1 spectral region for n − 6, n − 3 and ratio of n − 6:n − 3 with high regression coefficients (R 2 ) of 0.999, 0.994 and 0.998, respectively and low RMSEP values of 1, 0.06 and 0.83, respectively. The results of the present study revealed that FTIR could be used for rapid and accurate determination of n − 6, n − 3 and ratio of n − 6:n − 3 PUFAs present in poultry feed lipids.
Spectrochimica Acta Part A-molecular and Biomolecular Spectroscopy, 2007
A very fast, economical and simpler direct spectrophotometric method was investigated for paracet... more A very fast, economical and simpler direct spectrophotometric method was investigated for paracetamol (PC) determination in aqueous medium without using any chemical reagents. The method is based on the photo-absorption of the analyte at 243 nm after dissolution in water. The change in structure of PC after addition of water was studied by comparing the corresponding FTIR spectra. Optimization studies were conducted by using a 5 microg ml(-1) standard solution of the analyte. Various parameters studied include, time for stability and measurement of spectra, effect of HCl, NaOH, CH(3)COOH and NH(3) for change in absorbance and shift in spectra, interference by some analgesic drugs and some polar solvents and temperature effect. After optimization, Beer's law was obeyed in the range of 0.3-20 microg ml(-1) PC solution with a correlation coefficient of 0.9999 and detection limit of 0.1 microg ml(-1). The newly developed method was successfully applied for PC determination in some locally available tablets and urine samples. The proposed method is very useful for quick analysis of various types of solid and liquid samples containing PC.