Abedin Vakili - Academia.edu (original) (raw)

Papers by Abedin Vakili

This study aimed to investigate whether a combination of two potent antioxidants, alpha-lipoic ac... more This study aimed to investigate whether a combination of two potent antioxidants, alpha-lipoic acid (ALA) and mitoquinone (Mito Q), could improve myocardial function and the underlying mechanisms in an experimental model of myocardial infarction in aged rats. To develop a myocardial infarction model in aged rats the left anterior descending artery (LADA) was transiently occluded for 30 minutes and then re-perfused for 24 hours. Mito Q (10 mg/kg, IP) and ALA (100 mg/kg, gavage) was given daily for 2 weeks before occlusion of LADA. Subsequently, 24 hours after ischemia, left ventricular function was measured, and inflammatory factors (IL-6, IL-1β, TNF-α), tissue apoptosis, expression of Bax, Bcl-2, cytochrome C (Cyt-c), and caspase-3 were evaluated using ELISA, TUNEL, real-time PCR methods, respectively. The findings of this study indicated that the administration of the combination of ALA and Mito Q significantly improved cardiac function. This improvement was linked to a reduction i...

Iranian Journal of Medical Sciences, 2005

Background: Pentoxifylline (PTX) is used in human for intermittent claudication and cerebral vasc... more Background: Pentoxifylline (PTX) is used in human for intermittent claudication and cerebral vascular disorders including cerebrovascular dementia. It also inhibits the synthesis of tumor necrosis factor-α (TNF-α), which is believed to be neurotoxic in animal models of cerebral ischemia. The objective of this study was to examine the role of PTX on ischemia/reperfusion injures in rat model of transient focal cerebral ischemia induced by middle cerebral artery occlusion (MCAO). Methods: Male Sprague Dawley rats (n=31) were assigned to sham, saline or PTX (30 or 60 mg/kg)-treated groups. Ischemia was induced by MCAO, followed by 24-hrs reperfusion. Intraperitoneal saline or PTX was administered at 30 min before ischemia. Neurological deficit score test (NDS) was performed after 24-hrs, and the animals was sacrificed for evaluation of cortical and striatal infarct volumes using triphenyltetrazolium chloride staining. Results: The sham group did not have neural dysfunction or cerebral ...

Neurochemical Research, 2021

The present study was conducted to investigate the effects of different doses of recombinant huma... more The present study was conducted to investigate the effects of different doses of recombinant human Chemerin (rhChemerin) on brain damage, spatial memory, blood-brain barrier (BBB) disruption and cellular and molecular mechanisms in a mouse stroke model. The mouse stroke model was developed by blocking the middle cerebral artery for 1 h and performing reperfusion for 23 h. Immediately, one and three hours after the stroke, 200, 400 and 800 ng/mouse of intranasal rhChemerin was administered. Neuronal and BBB damage, spatial memory and neurological performance were examined 24 h after the stroke. Western blotting and immunofluorescence were utilized to determine the effects of rhChemerin on the expressions of nuclear factor kappa B (NF-κB), pro-inflammatory cytokines such as TNF-α and IL-1β, anti-inflammatory cytokines such as IL-10 and TGF-β and vascular endothelial growth factor (VEGF). Administering 400 and 800 ng/mouse of rhChemerin in the mice immediately and one hour after ischemia minimized the infarct size, BBB opening, spatial memory and neurological impairment (P < 0.001). Furthermore, 800 ng/mouse of rhChemerin significantly diminished terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)-positive (apoptotic) cells, suppressed the expressions of NF-kB, TNF-α and IL-1β and upregulated IL-10 and VEGF in the cortex and hippocampus of the mice. The present findings showed that rhChemerin administered immediately and one hour after stroke alleviates neuronal and BBB injures and improves spatial memory. These effects of rhChemerin may be mediated by inhibiting inflammatory pathways and apoptotic machinery.

Tehran University Medical Journal (TUMJ), 2008

Page 1. نﺎﻴﻗدﺎﺻ ﺪﻴﻌﺳ نارﺎﻜﻤﻫ و ﺪﻜﺸﻧاد ﻪﻠﺠﻣ هرود ،ناﺮﻬﺗ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﻲﻜﺷﺰﭘ ه 66 ، هرﺎﻤﺷ 8، ... more Page 1. نﺎﻴﻗدﺎﺻ ﺪﻴﻌﺳ نارﺎﻜﻤﻫ و ﺪﻜﺸﻧاد ﻪﻠﺠﻣ هرود ،ناﺮﻬﺗ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﻲﻜﺷﺰﭘ ه 66 ، هرﺎﻤﺷ 8، نﺎﺑآ 1387 يد تاﺮﺛا ﻲﺳرﺮﺑ ﻧ تﻻﻼﺘﺧا و تﺎﻌﻳﺎﺿ ﺮﺑ ﺪﻳﺎﺴﻛﻮﻔﻟﻮﺳ ﻞﻴﺘﻣ ﻮ ﻲﻌﺿﻮﻣ يﺰﻐﻣ ﻲﻤﻜﺴﻳا رد ﻚﻳژﻮﻟور ﻲﺘﻗﻮﻣ ﻲﻳاﺮﺤﺻ شﻮﻣ رد ﻲﻠﻴﻛو ﻦﻳﺪﺑﺎﻋ * هوﺮﮔ يژﻮﻟﻮﻳﺰﻴﻓ ، نﺎﻨﻤﺳ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ، ﻲﻜﺷﺰﭘ هﺪﻜﺸﻧاد ، ﺰﻛﺮﻣ ﺶﺨﺑ و تﺎﻘﻴﻘﺤﺗ يژﻮﻟﻮﻳﺰﻴﻓ ...

Journal of Medicinal Plants, 2012

Background: Ferula persica has been used in traditional medicine for treatment of high blood pres... more Background: Ferula persica has been used in traditional medicine for treatment of high blood pressure. In this study acute and chronic effect of aqueous F. persica extract on BP of hypertensive rats and its possible mechanism of action have been investigated. Methods: Eighty two male Wistar rats were divided into 12 experimental groups. Hypertension was induced by Goldblatt method in the anesthetized rats. Aqueous extract of F. persica (15 or 30 or 60 mg/kg, iv) or it’s vehicle were administered in treatments or control groups to evaluate their effects on BP and heart rate. To assess the mechanism of F. persica action on BP, L-NAME (5 mg/kg), Atropine (1 mg/kg) or Indomethacin (5 mg/kg) were injected intraperitoneally followed by intravenous administration of F. persica (30 mg/kg) in the different groups of hypertensive rats. Chronic effect of F. persica (30 mg/kg) on BP was evaluated by the aqueous extract administration in drinking water for a month. Results: Intravenous administr...

Journal of Medicinal Plants, 2015

Journal of stroke and cerebrovascular diseases, Nov 1, 2018

Our recent research showed that resistin has a neuroprotective effect against stroke-induced inju... more Our recent research showed that resistin has a neuroprotective effect against stroke-induced injury through suppressing apoptosis and oxidative stress. However, the molecular mechanism of neuroprotection of resistin is unclear. This work was designed to examine the effect of mouse recombinant resistin on mRNA expression of Tumor necrosis factor-α (TNF-α), Interleukin-1β (IL-1β), Interleukin-10 (IL-10), Transforming growth factor-β1 (TGF- β1), and Heat shock protein-70 (HSP-70) in mouse model of stroke. Transient focal cerebral ischemia was induced by the middle cerebral artery occlusion (MCAO) in mice. TNF-α, IL-1β, IL-10, TGF-β1, and HSP-70 mRNA were detected at sham (0 hour), 3 hours, 6 hours, 12 hours, and 24 hours after MCAO using real-time QRT-PCR method. Moreover, animals were treated with resistin at the dose of 400 ng/mouse at the commencement of MCAO, and mRNA expression of the cytokines and HSP-70 was measured 24 hours after MCAO. Tumor necrosis factor-α and IL-1β mRNA expression markedly increased at 12-hour time point and then returned to the basal level at 24 hours after MCAO; but HSP-70 mRNA expression increased at 24-hour time point. Furthermore, resistin (400 ng/mouse) significantly increased TGF-β1 and IL-10 and decreased HSP-70 gene expression at 24 hours after MCAO. Our findings revealed that a molecular mechanism of attenuating ischemic damage by resistin administration probably is increased mRNA expression of anti-inflammatory cytokines. However, applying resistin in the clinical settings for the treatment of stroke deserves further researches in the future.

Basic and clinical neuroscience, Nov 30, 2019

Based on our previous findings, the treatment of stem cells alone or in combination with thyroid ... more Based on our previous findings, the treatment of stem cells alone or in combination with thyroid hormone (T 3) and mild exercise could effectively reduce the risk of stroke damage in young mice. However, it is unclear whether this treatment is effective in aged or middle-aged mice. Therefore, this study designed to assess whether combination of Bone Marrow Stromal Cells (BMSCs) with T 3 and mild treadmill exercise can decrease stroke complications in middle-aged mice. Methods: Under laser Doppler flowmetry monitoring, transient focal cerebral ischemia was produced by right Middle Cerebral Artery Occlusion (MCAO) for 45 min followed by 7 days of reperfusion in middle-aged mice. BMSCs (1×10 5) were injected into the right cerebral ventricle 24 h after MCAO, followed by daily injection of triiodothyronine (T 3) (20 µg/100 g/d SC) and 6 days of running on a treadmill. Infarct size, neurological function, apoptotic cells and expression levels of Glial Fibrillary Acidic Protein (GFAP) were evaluated 1 week after stroke. Results: Post-ischemic treatment with BMSCs or with T 3 and or mild treadmill exercise alone or in combination did not significantly change neurological function, infarct size, and apoptotic cells 7 days after ischemia in middle-aged mice (P>0.05). However, the expression of GFAP significantly reduced after treatment with BMSCs and or T 3 (P<0.01). Conclusion: Our findings indicate that post-stroke treatment BMSCs with exercise and thyroid hormone cannot reverse neuronal damage 7 days after ischemia in middle-aged mice. These findings further support that age is an important variable in stroke treatment Article info:

Clinical and Experimental Pharmacology and Physiology, Dec 17, 2019

The renin-angiotensin system (RAS) has a deleterious and apelin/APJ system has protective effect ... more The renin-angiotensin system (RAS) has a deleterious and apelin/APJ system has protective effect on the ischemic heart. The collaboration between these systems in the pathophysiology of myocardial infarction is not clear. We determined the effect of chronic pretreatment with apelin, losartan and their combination on ischemia-reperfusion (IR) injury in the isolated perfused rat heart and on the expression of apelin-13 receptor (APJ) and angiotensin type 1 receptor (AT1R) in the myocardium. During five days before the induction of IR, saline (vehicle), apelin-13 (Apl), F13A (apelin antagonist), losartan (Los, AT1R antagonist) and the combination of Apl and Los were administered intraperitoneally in rats. Ischemia was induced by left anterior descending (LAD) artery occlusion for 30min followed by reperfusion for 55min in the Langendorff isolated heart perfusion system. Pretreatment with Apl, Los and the combination of Apl+Los significantly reduced infarct size by about 30, 33 and 48 percent respectively; and significantly improved the left ventricular function indices such as left ventricular developed pressure (LVDP), left ventricular end diastolic pressure (LVEDP) and rate pressure product (RPP). IR increased AT1R protein level but it did not change APJ significantly. AT1R expression was Accepted Article This article is protected by copyright. All rights reserved reduced in groups treated with Apl, Los and Apl+Los. Findings showed that Chronic pretreatment with apelin along with AT1R antagonist had more protective effects against IR injury. Combination therapy may diminish the risk of IR-induced heart damage, by reducing AT1R expression, in the heart of patients with coronary artery disease that are at the risk of MI and reperfusion injury.

Neurochemistry International, Mar 1, 2018

New evidence suggests that resistin may have a therapeutic potential effect in management of neur... more New evidence suggests that resistin may have a therapeutic potential effect in management of neurodegenerative disease; but its role in the pathophysiology of stroke-induced injuries is not understood. However, further investigations are required to elucidate the effect of resistin and explore its possible molecular mechanisms on the ischemic reperfusion injury. Transient focal cerebral ischemia was induced by the middle cerebral artery occlusion (MCAO) in mice. Animal treated with resistin at doses of 25, 50, 100, 200, and 400 ng/mouse, on the MCAO commencement. Neurological function, infarct size, brain edema and Blood-brain barrier (BBB) disruption were measured. Additionally, content of malondialdehyde (MDA), TUNEL-positive cells and apoptosis-related proteins were assessed by immunohistochemistry and western blot techniques. Resistin mRNA was detected at 3 h, 6 h, 12 h and 24 h after MCAO using real-time QRT-PCR method. Central administration of resistin only at doses of 200 and 400 ng/mouse considerably reduced the infarct size and promoted neurological function (p < 0.001). In addition, resistin (400 ng/mouse) significantly decreased brain edema (p < 0.001), evans blue (EB) leakage (p < 0.05), MDA content (p < 0.005), apoptotic cells and apoptosis-related proteins (p < 0.001). Resistin mRNA expression markedly increased at 12-h time point and then returned to basal level at 24 h after MCAO. Our findings revealed that treatment with resistin could attenuate ischemic damage in a dose-dependent approach via suppressing apoptosis and oxidative stress. Application of resistin in clinical settings to treat stroke and brain ischemia warrants further research.

Journal of stroke and cerebrovascular diseases, Dec 1, 2015

Hydrogen sulfide (H2S) plays multiple roles in the function of the central nervous system in phys... more Hydrogen sulfide (H2S) plays multiple roles in the function of the central nervous system in physiological and pathological conditions, such as cerebral ischemia. Recent studies have reported controversial results about the role of H2S in cerebral ischemia. The aim of this study was to evaluate the effects of amino-oxyacetic acid (AOAA), an inhibitor of H2S synthesis, on ischemic injury in an experimental model of stroke. Using laser Doppler monitoring, cerebral ischemia was induced by transient middle cerebral artery occlusion (MCAO) for 1 hour in rats. AOAA (.025, .05, .1, and .5 mmol/kg intraperitoneally [i.p.]) was injected at the beginning of MCAO. Infarct volume, cerebral edema, and activity of antioxidant enzymes were measured using the standard methods 24 hours after ischemia. The administration of AOAA at doses .025, .05, and .1 mmol/kg significantly reduced the infarct volume (P &amp;amp;amp;amp;amp;amp;amp;lt; .001). Furthermore, .025 and .05 mmol/kg of AOAA significantly reduced brain edema and improved the neurological outcome (P &amp;amp;amp;amp;amp;amp;amp;lt; .001). The administration of AOAA did not significantly change the malondialdehyde content, activities of superoxide dismutase, or glutathione peroxidase antioxidant enzymes in the brain tissue (P &amp;amp;amp;amp;amp;amp;amp;gt; .05). The results showed that AOAA administered at a low dose has protective effects; however, at higher doses it did not exert any protective effect against cerebral ischemia and even worsened the ischemic injury. This finding suggests that H2S might be both beneficial and harmful in cerebral ischemic injury depending on its concentration in transient model of focal cerebral ischemia.

Molecular Biology Reports, Jul 8, 2019

Probiotics are referred to species of living microscopic organisms may help conserve the normal b... more Probiotics are referred to species of living microscopic organisms may help conserve the normal balance of the digestive system and/or manage diseases. A number of autoimmune, psychiatric, cardiovascular and cerebrovascular disorders may be associated with the imbalance of gut microbiota. This study examines the effect of 21 days consumption of multistrain probiotics on hippocampus injury, spatial and learning memory and some potential molecular mechanisms in a mouse model with cerebral hypoperfusion. Cerebral hypoperfusion was established in the mouse model by bilateral common carotid artery occlusion (BCCAO) for 20 min and 24 h reperfusion. Mixtures of several probiotic bacteria at concentrations of 10 7 , 10 8 and 10 9 CFU/day were orally administrated for 3 weeks before the BCCAO. Spatial and learning memory, histological damage and apoptosis were assessed in the CA1, CA3 and dentate gyrus (DG) of the hippocampus 24 h after ischemia. The malondialdehyde (MDA) content and brain-derived neurotrophic factor (BDNF) level were measured by ELISA technique. Prophylactic of probiotic considerably reduced the number of apoptotic cells and neuronal death in the CA1, CA3 and DG of the hippocampus at all three concentrations (P < 0.001). In addition, probiotics reduced spatial memory impairment and neurological dysfunction only at the 10 9-CFU/day (P < 0.01). Nonetheless, probiotics did not change the levels of BDNF and MDA in the hippocampus (P > 0.05). According to the findings, the daily prophylactic ingestion of probiotics reduced hippocampus damage and prevented the spatial learning and memory deficit by suppressing apoptosis in the mouse model with cerebral hypoperfusion. Probiotic supplementation may be suggested as a useful preventive dietary strategy for groups susceptible to cerebrovascular diseases.

Jundishapur Journal of Natural Pharmaceutical Products, Dec 12, 2019

Background: Some studies have shown therapeutic properties of Prangos ferulacea, but its effect o... more Background: Some studies have shown therapeutic properties of Prangos ferulacea, but its effect on blood pressure (BP) is unidentified. Objectives: So acute and chronic feeding effects of P. ferulacea on BP and its mechanism were evaluated in male rats. Methods: Hydroalcoholic extract of P. ferulacea (12.5, 25, and 50 mg/kg) was injected intravenously and mean arterial BP and heart rate were measured. The mechanism of effect of the extract on BP was evaluated by intraperitoneal administration of L-NAME, atropine or indomethacin (4, 1, and 5 mg/kg, respectively) and then intravenous injection of extract (50 mg/kg). In addition, the extract (500 mg/kg/day) was given orally in L-NAME (40 mg/kg/day)-induced hypertensive rats during 4 weeks. Then anxiety behaviors and BP were assessed. Results: Intravenous P. ferulacea reduced BP significantly (P < 0.01). There was a significant difference between the effect of 12.5 and 50 mg/kg of extract (P < 0.001). The effect of hypotension of the extract was eliminated by atropine and decreased by L-NAME. Chronic administration of L-NAME increased BP from 112 mmHg in the control group to 149 in hypertensive rats (P < 0.01), while the oral extract in these rats reduced BP to 119 mmHg (P < 0.01). The P. ferulacea had no effect on heart rate and anxiety behaviors in normal and hypertensive rats. Conclusions: Intravenous P. ferulacea reduces BP, which may be via the muscarinic receptor. Oral P. ferulacea prevents BP augmentation induced by L-NAME. The P. ferulacea seems to be useful for prophylaxis of hypertension.

Tehran University Medical …, 2011

Background: Numerous studies have shown the protective effects of saffron against oxidative damag... more Background: Numerous studies have shown the protective effects of saffron against oxidative damage in a global model of cerebral ischemia, but its effects on brain edema and oxidative ischemic injury in focal ischemic stroke are not completely understood. Therefore, this study was designed to investigate the effects of saffron on brain edema, infarct volume, antioxidant enzyme activity (glutathione peroxidase and superoxide dismutase) and concentration of malondialdehyde (MDA) in ischemic brain tissue in an experimental model of stroke. Methods: Focal brain ischemia was established with the temporary occlusion of the middle cerebral artery for one hour in rats. Saffron (100 mg/kg) was given intraperitoneally at the onset of ischemia. 24 hours later, brain edema and infarct volume were evaluated and glutathione peroxidase and superoxide dismutase activities and MDA concentration were measured in the ischemic brain tissue using a specific kit. Results: The results showed that saffron reduced infarct volume by 77% (P<0.001) and brain edema by 60% (P<0.001) compared with the control group in 24 hours following ischemia. Moreover, saffron significantly reduced the content of MDA (P<0.001) and increased the activity of superoxide dismutase (P<0.001) and glutathione peroxidase (P<0.001) in the cortex of the ischemic brain tissue. Conclusion: Saffron has protective effects against oxidative ischemic damage and brain edema in a transient model of focal cerebral ischemia in rats. This protective effect is probably induced by increasing the capacity of antioxidant enzymes and decreasing the production of free radicals.

Journal of Medical Sciences(Faisalabad), 2008

Journal of Gorgan University of Medical Sciences, 2015

Background and Objective: Several studies have shown that inhalation of Lavandula angustifolia L.... more Background and Objective: Several studies have shown that inhalation of Lavandula angustifolia L. (Lavender) reduces hypertension, while systemic effects and mechanism of action of lavender oil on blood pressure is not clear. This study was carried out to evaluate the effect of intravenous and ntraperitoneal injection of Lavandula angustifolia L. oil on normal blood pressure in male rats. Methods: In this experimental study, 70 male Wistar rats were randomly allocated into 10 groups (n=7). Following anesthetizing the animals with sodium thiopental, femoral artery and vein were cannulated respectively for recording blood pressure and injection of Lavandula angustifolia L. oil. Lavender oil or its vehicle (Propylene glycol) was injected by intravenous (25, 50 and 100 mg/kg/bw) or intraperitoneal injection (500mg/kg/bw). For the evaluation of the mechanism of Lavender oil, L-NAME (4mg/kg/bw), atropine (1mg/kg/bw), indomethacin (5 mg/kg/bw) or saline was injected intraperitoneally befor...

Cell journal, Jun 1, 2022

Despite extensive medical advances, stroke is still one of the major problems in health care syst... more Despite extensive medical advances, stroke is still one of the major problems in health care system. Researchers are seeking novel therapeutic strategies for the treatment of stroke, such as cell-therapy. In this regard, mesenchymal stem cells are the most used cells in stroke cell-therapy researches. Mesenchymal stem cells are multipotent cells capable of self-renewal and differentiation, which can be derived from various tissues, such as bone marrow, skeletal muscle, adipose tissue, umbilical cord, and synovium. In this review article, after description of mesenchymal stem cells, the studies related to use of these cells in stroke as well as the challenges ahead in the field of stroke cell therapy, were mentioned. According to existing studies, although it seems that use of mesenchymal stem cells transplantation has a bright prospect in the treatment of stroke, there are still some issues, such as apoptosis of grafted cell, neural differentiation of stem cell, likelihood of malign...

Middle East Journal of Rehabilitation and Health Studies, 2022

Background: We have already found that post-ischemic intervention with recombinant human chemerin... more Background: We have already found that post-ischemic intervention with recombinant human chemerin (rh-chemerin) can protect neurons against cerebral ischemic. Objectives: In this study, we tested whether pretreatment with rh-chemerin could reduce brain damage and spatial memory impairment in a model of stroke in mice. Methods: A stroke model was produced by middle cerebral artery occlusion (MCAO) for 60 minutes and 24 hours reperfusion in mice. In the present research, 23 mice were randomly divided into 3 groups, including the sham-operated group (surgery + no MCAO; n = 7), control group (MCAO + saline; n = 8), treatment group (MCAO + rh-chemerin; n = 8). Rh-chemerin (800 ng/mouse) was given intranasally for 7 consecutive days before MCAO. Infarct size and spatial learning and memory were assessed by the 2,3,5-triphenyltetrazolium chloride (TTC) staining method and radial arm water maze (RAWM) device, respectively, at 24 hours after ischemia. Results: The blockade of MCA caused seve...