Abedin Vakili - Academia.edu (original) (raw)

Papers by Abedin Vakili

This study aimed to investigate whether a combination of two potent antioxidants, alpha-lipoic ac... more This study aimed to investigate whether a combination of two potent antioxidants, alpha-lipoic acid (ALA) and mitoquinone (Mito Q), could improve myocardial function and the underlying mechanisms in an experimental model of myocardial infarction in aged rats. To develop a myocardial infarction model in aged rats the left anterior descending artery (LADA) was transiently occluded for 30 minutes and then re-perfused for 24 hours. Mito Q (10 mg/kg, IP) and ALA (100 mg/kg, gavage) was given daily for 2 weeks before occlusion of LADA. Subsequently, 24 hours after ischemia, left ventricular function was measured, and inflammatory factors (IL-6, IL-1β, TNF-α), tissue apoptosis, expression of Bax, Bcl-2, cytochrome C (Cyt-c), and caspase-3 were evaluated using ELISA, TUNEL, real-time PCR methods, respectively. The findings of this study indicated that the administration of the combination of ALA and Mito Q significantly improved cardiac function. This improvement was linked to a reduction i...

Iranian Journal of Medical Sciences, 2005

Background: Pentoxifylline (PTX) is used in human for intermittent claudication and cerebral vasc... more Background: Pentoxifylline (PTX) is used in human for intermittent claudication and cerebral vascular disorders including cerebrovascular dementia. It also inhibits the synthesis of tumor necrosis factor-α (TNF-α), which is believed to be neurotoxic in animal models of cerebral ischemia. The objective of this study was to examine the role of PTX on ischemia/reperfusion injures in rat model of transient focal cerebral ischemia induced by middle cerebral artery occlusion (MCAO). Methods: Male Sprague Dawley rats (n=31) were assigned to sham, saline or PTX (30 or 60 mg/kg)-treated groups. Ischemia was induced by MCAO, followed by 24-hrs reperfusion. Intraperitoneal saline or PTX was administered at 30 min before ischemia. Neurological deficit score test (NDS) was performed after 24-hrs, and the animals was sacrificed for evaluation of cortical and striatal infarct volumes using triphenyltetrazolium chloride staining. Results: The sham group did not have neural dysfunction or cerebral ...

Neurochemical Research, 2021

The present study was conducted to investigate the effects of different doses of recombinant huma... more The present study was conducted to investigate the effects of different doses of recombinant human Chemerin (rhChemerin) on brain damage, spatial memory, blood-brain barrier (BBB) disruption and cellular and molecular mechanisms in a mouse stroke model. The mouse stroke model was developed by blocking the middle cerebral artery for 1 h and performing reperfusion for 23 h. Immediately, one and three hours after the stroke, 200, 400 and 800 ng/mouse of intranasal rhChemerin was administered. Neuronal and BBB damage, spatial memory and neurological performance were examined 24 h after the stroke. Western blotting and immunofluorescence were utilized to determine the effects of rhChemerin on the expressions of nuclear factor kappa B (NF-κB), pro-inflammatory cytokines such as TNF-α and IL-1β, anti-inflammatory cytokines such as IL-10 and TGF-β and vascular endothelial growth factor (VEGF). Administering 400 and 800 ng/mouse of rhChemerin in the mice immediately and one hour after ischemia minimized the infarct size, BBB opening, spatial memory and neurological impairment (P < 0.001). Furthermore, 800 ng/mouse of rhChemerin significantly diminished terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)-positive (apoptotic) cells, suppressed the expressions of NF-kB, TNF-α and IL-1β and upregulated IL-10 and VEGF in the cortex and hippocampus of the mice. The present findings showed that rhChemerin administered immediately and one hour after stroke alleviates neuronal and BBB injures and improves spatial memory. These effects of rhChemerin may be mediated by inhibiting inflammatory pathways and apoptotic machinery.

Tehran University Medical Journal (TUMJ), 2008

Page 1. نﺎﻴﻗدﺎﺻ ﺪﻴﻌﺳ نارﺎﻜﻤﻫ و ﺪﻜﺸﻧاد ﻪﻠﺠﻣ هرود ،ناﺮﻬﺗ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﻲﻜﺷﺰﭘ ه 66 ، هرﺎﻤﺷ 8، ... more Page 1. نﺎﻴﻗدﺎﺻ ﺪﻴﻌﺳ نارﺎﻜﻤﻫ و ﺪﻜﺸﻧاد ﻪﻠﺠﻣ هرود ،ناﺮﻬﺗ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﻲﻜﺷﺰﭘ ه 66 ، هرﺎﻤﺷ 8، نﺎﺑآ 1387 يد تاﺮﺛا ﻲﺳرﺮﺑ ﻧ تﻻﻼﺘﺧا و تﺎﻌﻳﺎﺿ ﺮﺑ ﺪﻳﺎﺴﻛﻮﻔﻟﻮﺳ ﻞﻴﺘﻣ ﻮ ﻲﻌﺿﻮﻣ يﺰﻐﻣ ﻲﻤﻜﺴﻳا رد ﻚﻳژﻮﻟور ﻲﺘﻗﻮﻣ ﻲﻳاﺮﺤﺻ شﻮﻣ رد ﻲﻠﻴﻛو ﻦﻳﺪﺑﺎﻋ * هوﺮﮔ يژﻮﻟﻮﻳﺰﻴﻓ ، نﺎﻨﻤﺳ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ، ﻲﻜﺷﺰﭘ هﺪﻜﺸﻧاد ، ﺰﻛﺮﻣ ﺶﺨﺑ و تﺎﻘﻴﻘﺤﺗ يژﻮﻟﻮﻳﺰﻴﻓ ...

Journal of Medicinal Plants, 2012

Background: Ferula persica has been used in traditional medicine for treatment of high blood pres... more Background: Ferula persica has been used in traditional medicine for treatment of high blood pressure. In this study acute and chronic effect of aqueous F. persica extract on BP of hypertensive rats and its possible mechanism of action have been investigated. Methods: Eighty two male Wistar rats were divided into 12 experimental groups. Hypertension was induced by Goldblatt method in the anesthetized rats. Aqueous extract of F. persica (15 or 30 or 60 mg/kg, iv) or it’s vehicle were administered in treatments or control groups to evaluate their effects on BP and heart rate. To assess the mechanism of F. persica action on BP, L-NAME (5 mg/kg), Atropine (1 mg/kg) or Indomethacin (5 mg/kg) were injected intraperitoneally followed by intravenous administration of F. persica (30 mg/kg) in the different groups of hypertensive rats. Chronic effect of F. persica (30 mg/kg) on BP was evaluated by the aqueous extract administration in drinking water for a month. Results: Intravenous administr...

Journal of Medicinal Plants, 2015

Journal of stroke and cerebrovascular diseases, Nov 1, 2018

Our recent research showed that resistin has a neuroprotective effect against stroke-induced inju... more Our recent research showed that resistin has a neuroprotective effect against stroke-induced injury through suppressing apoptosis and oxidative stress. However, the molecular mechanism of neuroprotection of resistin is unclear. This work was designed to examine the effect of mouse recombinant resistin on mRNA expression of Tumor necrosis factor-α (TNF-α), Interleukin-1β (IL-1β), Interleukin-10 (IL-10), Transforming growth factor-β1 (TGF- β1), and Heat shock protein-70 (HSP-70) in mouse model of stroke. Transient focal cerebral ischemia was induced by the middle cerebral artery occlusion (MCAO) in mice. TNF-α, IL-1β, IL-10, TGF-β1, and HSP-70 mRNA were detected at sham (0 hour), 3 hours, 6 hours, 12 hours, and 24 hours after MCAO using real-time QRT-PCR method. Moreover, animals were treated with resistin at the dose of 400 ng/mouse at the commencement of MCAO, and mRNA expression of the cytokines and HSP-70 was measured 24 hours after MCAO. Tumor necrosis factor-α and IL-1β mRNA expression markedly increased at 12-hour time point and then returned to the basal level at 24 hours after MCAO; but HSP-70 mRNA expression increased at 24-hour time point. Furthermore, resistin (400 ng/mouse) significantly increased TGF-β1 and IL-10 and decreased HSP-70 gene expression at 24 hours after MCAO. Our findings revealed that a molecular mechanism of attenuating ischemic damage by resistin administration probably is increased mRNA expression of anti-inflammatory cytokines. However, applying resistin in the clinical settings for the treatment of stroke deserves further researches in the future.

Basic and clinical neuroscience, Nov 30, 2019

Clinical and Experimental Pharmacology and Physiology, Dec 17, 2019

Neurochemistry International, Mar 1, 2018

Journal of stroke and cerebrovascular diseases, Dec 1, 2015

Hydrogen sulfide (H2S) plays multiple roles in the function of the central nervous system in phys... more Hydrogen sulfide (H2S) plays multiple roles in the function of the central nervous system in physiological and pathological conditions, such as cerebral ischemia. Recent studies have reported controversial results about the role of H2S in cerebral ischemia. The aim of this study was to evaluate the effects of amino-oxyacetic acid (AOAA), an inhibitor of H2S synthesis, on ischemic injury in an experimental model of stroke. Using laser Doppler monitoring, cerebral ischemia was induced by transient middle cerebral artery occlusion (MCAO) for 1 hour in rats. AOAA (.025, .05, .1, and .5 mmol/kg intraperitoneally [i.p.]) was injected at the beginning of MCAO. Infarct volume, cerebral edema, and activity of antioxidant enzymes were measured using the standard methods 24 hours after ischemia. The administration of AOAA at doses .025, .05, and .1 mmol/kg significantly reduced the infarct volume (P &amp;amp;amp;amp;amp;amp;amp;lt; .001). Furthermore, .025 and .05 mmol/kg of AOAA significantly reduced brain edema and improved the neurological outcome (P &amp;amp;amp;amp;amp;amp;amp;lt; .001). The administration of AOAA did not significantly change the malondialdehyde content, activities of superoxide dismutase, or glutathione peroxidase antioxidant enzymes in the brain tissue (P &amp;amp;amp;amp;amp;amp;amp;gt; .05). The results showed that AOAA administered at a low dose has protective effects; however, at higher doses it did not exert any protective effect against cerebral ischemia and even worsened the ischemic injury. This finding suggests that H2S might be both beneficial and harmful in cerebral ischemic injury depending on its concentration in transient model of focal cerebral ischemia.

Molecular Biology Reports, Jul 8, 2019

Jundishapur Journal of Natural Pharmaceutical Products, Dec 12, 2019

Tehran University Medical …, 2011

Journal of Medical Sciences(Faisalabad), 2008

Journal of Gorgan University of Medical Sciences, 2015

Background and Objective: Several studies have shown that inhalation of Lavandula angustifolia L.... more Background and Objective: Several studies have shown that inhalation of Lavandula angustifolia L. (Lavender) reduces hypertension, while systemic effects and mechanism of action of lavender oil on blood pressure is not clear. This study was carried out to evaluate the effect of intravenous and ntraperitoneal injection of Lavandula angustifolia L. oil on normal blood pressure in male rats. Methods: In this experimental study, 70 male Wistar rats were randomly allocated into 10 groups (n=7). Following anesthetizing the animals with sodium thiopental, femoral artery and vein were cannulated respectively for recording blood pressure and injection of Lavandula angustifolia L. oil. Lavender oil or its vehicle (Propylene glycol) was injected by intravenous (25, 50 and 100 mg/kg/bw) or intraperitoneal injection (500mg/kg/bw). For the evaluation of the mechanism of Lavender oil, L-NAME (4mg/kg/bw), atropine (1mg/kg/bw), indomethacin (5 mg/kg/bw) or saline was injected intraperitoneally befor...

Cell journal, Jun 1, 2022

Despite extensive medical advances, stroke is still one of the major problems in health care syst... more Despite extensive medical advances, stroke is still one of the major problems in health care system. Researchers are seeking novel therapeutic strategies for the treatment of stroke, such as cell-therapy. In this regard, mesenchymal stem cells are the most used cells in stroke cell-therapy researches. Mesenchymal stem cells are multipotent cells capable of self-renewal and differentiation, which can be derived from various tissues, such as bone marrow, skeletal muscle, adipose tissue, umbilical cord, and synovium. In this review article, after description of mesenchymal stem cells, the studies related to use of these cells in stroke as well as the challenges ahead in the field of stroke cell therapy, were mentioned. According to existing studies, although it seems that use of mesenchymal stem cells transplantation has a bright prospect in the treatment of stroke, there are still some issues, such as apoptosis of grafted cell, neural differentiation of stem cell, likelihood of malign...

Middle East Journal of Rehabilitation and Health Studies, 2022

Background: We have already found that post-ischemic intervention with recombinant human chemerin... more Background: We have already found that post-ischemic intervention with recombinant human chemerin (rh-chemerin) can protect neurons against cerebral ischemic. Objectives: In this study, we tested whether pretreatment with rh-chemerin could reduce brain damage and spatial memory impairment in a model of stroke in mice. Methods: A stroke model was produced by middle cerebral artery occlusion (MCAO) for 60 minutes and 24 hours reperfusion in mice. In the present research, 23 mice were randomly divided into 3 groups, including the sham-operated group (surgery + no MCAO; n = 7), control group (MCAO + saline; n = 8), treatment group (MCAO + rh-chemerin; n = 8). Rh-chemerin (800 ng/mouse) was given intranasally for 7 consecutive days before MCAO. Infarct size and spatial learning and memory were assessed by the 2,3,5-triphenyltetrazolium chloride (TTC) staining method and radial arm water maze (RAWM) device, respectively, at 24 hours after ischemia. Results: The blockade of MCA caused seve...